arhgef12 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef12
Entrez Gene ID	779951
Full Name	Rho guanine nucleotide exchange factor 12
Synonyms	Larg,pro2792
General protein information	Preferred Names Rho guanine nucleotide exchange factor 12 Names rho guanine nucleotide exchange factor 12 Rho guanine nucleotide exchange factor (GEF) 12
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031905188.1	XP_031761048.1	rho guanine nucleotide exchange factor 12 isoform X5
XM_031905186.1	XP_031761046.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_031905187.1	XP_031761047.1	rho guanine nucleotide exchange factor 12 isoform X4
NM_001079028.1	NP_001072496.1	rho guanine nucleotide exchange factor 12
XM_031905184.1	XP_031761044.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_031905189.1	XP_031761049.1	rho guanine nucleotide exchange factor 12 isoform X6
XM_031905185.1	XP_031761045.1	rho guanine nucleotide exchange factor 12 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGEF12	XP_005619719.1
	ARHGEF12	XP_002799913.1
Gallus gallus (chicken)	ARHGEF12	XP_417890.4
Danio rerio (zebrafish)	arhgef12	XP_005172066.1
Pan troglodytes (chimpanzee)	ARHGEF12	XP_001166830.1
Xenopus tropicalis (tropical clawed frog)	arhgef12	NP_001072496.1
Homo sapiens (human)	ARHGEF12	NP_056128.1
Bos taurus (cattle)	ARHGEF12	NP_001116505.1
Mus musculus (house mouse)	Arhgef12	NP_081420.2
Rattus norvegicus (Norway rat)	Arhgef12	NP_001013264.1
Danio rerio (zebrafish)	LOC562455	XP_003200092.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following arhgef12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa61521	XM_031905184.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61522	XM_031905185.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61523	XM_031905186.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61524	XM_031905187.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61525	XM_031905188.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61526	XM_031905189.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa22766	NM_001079028.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef12 ( XM_031905184.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761044.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61521
Clone ID Related Accession (Same CDS sequence)	XM_031905184.1
Accession Version	XM_031905184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GCAGGTTTAC TGACCTGGGA 
ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT 
GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG 
GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG 
TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG 
CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA 
GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT 
TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG 
GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG 
GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA 
GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG 
AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT 
GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT 
CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC 
CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG 
ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA 
ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC 
AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC 
TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG 
GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC 
TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG 
ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA 
GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC 
TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC 
AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG 
CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA 
GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG 
AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC 
GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC 
AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT 
GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA 
GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG 
GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT 
AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT 
ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC 
TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT 
TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761044.1
CDS	779..5095
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATESSSRKTKSTWDESRQETYGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADSRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905184.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905184.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GCAGGTTTAC TGACCTGGGA 
ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT 
GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG 
GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG 
TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG 
CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA 
GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT 
TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG 
GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG 
GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA 
GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG 
AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT 
GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT 
CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC 
CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG 
ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA 
ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC 
AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC 
TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG 
GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC 
TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG 
ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA 
GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC 
TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC 
AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG 
CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA 
GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG 
AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC 
GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC 
AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT 
GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA 
GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG 
GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT 
AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT 
ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC 
TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT 
TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_031905185.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761045.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa61522
Clone ID Related Accession (Same CDS sequence)	XM_031905185.1
Accession Version	XM_031905185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT 
TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA 
GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT 
GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG 
GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA 
GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC 
CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG 
GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA 
ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA 
GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA 
CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC 
TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC 
TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG 
GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA 
TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG 
AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT 
GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG 
AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT 
AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA 
TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC 
ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT 
CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT 
GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA 
CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGCA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761045.1
CDS	779..5092
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATESSSRKTKSTWDESRQETYGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADSRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905185.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905185.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT 
TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA 
GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT 
GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG 
GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA 
GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC 
CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG 
GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA 
ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA 
GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA 
CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC 
TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC 
TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG 
GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA 
TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG 
AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT 
GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG 
AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT 
AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA 
TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC 
ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT 
CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT 
GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA 
CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGCA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_031905186.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761046.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.

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CloneID	OXa61523
Clone ID Related Accession (Same CDS sequence)	XM_031905186.1
Accession Version	XM_031905186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761046.1
CDS	779..5092
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATESSSRKTKSTWDESRQETYGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905186.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905186.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_031905187.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761047.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.

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CloneID	OXa61524
Clone ID Related Accession (Same CDS sequence)	XM_031905187.1
Accession Version	XM_031905187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT 
TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA 
GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT 
GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG 
GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA 
GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC 
CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG 
GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA 
ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA 
GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA 
CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC 
TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC 
TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG 
GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA 
TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG 
AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT 
GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG 
AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT 
AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA 
TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC 
ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT 
CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT 
GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA 
CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGGT TTACTGACCT GGGAACACCA 
AAACCTTTCA GAAAGTTGGA TAGCCTGGTT GTAGGTGACA CTCAGAGTGA GGATGAGCAC 
CATGAGATTG ACAAGGAACA AGATCCACCC AACTGGCAGC AGCTTGTTAG TAAGGAAATA 
CTGCAGAAAC TTAAACCGCA CGAGATCAAG CAACAGGAAG TCATACACGA GCTGTTTTAC 
ACTGAAAGGG CACATGTACA GAAACTGGGT GTTTTGGACC AAGTGTTTCA TCAGCGCGTG 
ATCCGTGAGA ATCTCTTAAC ACCTGCAGAG ACCCGAAATG TTTTCTCCAA CTTAGAAGAA 
ATCTTACAGC TTCATGTGGC ACTTAATGAG CAAATGAAAG CAGTAAAGAA GCGTTATGAA 
ACATCTGTGG TGGAACAAAT TGGAGATGAT GTTCTGAACT GGTTCAGTGG AGCGGAAGAG 
GAGAAGCTAA AGCAGGCAGT GGCCACATTT TGCAGTAACC AGCCCTTTGC GTTGGAGATG 
ATAAAATCTC GCCAAAAGAA AGATCTAAAA TTCCTAACCT TTGTACAGGA TGCAGAGAGT 
AATCCTCTGT GTAAGCGCCT GCAGTTGAAG GATATTATTC CCACTGAGAT GCAGAGACTA 
ACCAAGTATC CACTGCTGCT GGATAACATT GCCAGATATT CAGATCGAGA AGATGAGAAA 
GAAAAGGTGA ACAGAGCAGC AAACCACTGC CGTAACATCT TGGACTACGT AAATCAGGCA 
GTTAAAGAGG CAGAAAACAA GCAGAGACTT GAAGATTATC AACGCAGGCT GGACCTGTCC 
TATTTGAAGC CAGGAGAGTA TCCCATGATA GACGAACTGA GAAATCTAGA TTTGACCAAG 
AGAAAATTGA TACATGAGGG GCCTCTGACA TGGAAGGTTA ATAGAGACAA ATCAATCGAT 
CTTTACTCTC TGCTGCTAGA GGACATTTTG GTTTTACTTC AGAGACAAGA TGACAAGTTG 
ATTTTGAGGT GTCACAGCAA GATTCTGGCT TCAACTTCAG ACAGCAAACA TATCTTCAGC 
CCTGTTATCA AACTCAACAC CGTGTTAGTA CGGCAGGTGG CAACAGATAA CAAGGCATTC 
TTTGTTATAT CCATGTCAAA AAATGGGGCA CAGATTTACG AGCTCATTGC CAACTCTGTT 
TCCGAGAAAA ATGGGTGGCA AGACCTCATA ACAAAAATGG CTGCAACATC GAAGACTGAA 
TATCCTCAAC CTACTCAGTT ACAACAGACT GCCCCAGAGA TGGTCAGCGA AGAAGAACAT 
GAAGATGTGG AGCAACAGAA AGTGTCACAT GATCAACAAG AAGATAGACT TATCTATAGG 
CACAGTACAG ACAAAGACTC AGACACCGAA AGTTTAGTTC AATCGAAATC CCCCAGCAGC 
CCCAGTCCCT CCTCCTGTCT TTCATCTACA GAGCATCACC TGGCAGTGAA CGCGCTCAGA 
AACTTGGGAC TGCTAAAACA GTTACTGGTG CAGCAGCTGA AGCTCCTTCA GAAAGAGGAA 
GCCGAGAACT TTCAACGATT TTGCAGCCGT AAAACAAAGG AGCAGATGAG GGAGAGTGAA 
AAACTTACTA AGTTTTCAAG CTTGGATAAG CAGAACTGGG GAAGCAGGGA GTCCGATTTG 
AACAAAGAAC TGCATGCTCA GCAAAGGGCC ACAGACAGAC GTTCTGAACA TAGCAACACT 
GGCCCAGACT GTGTTGCTGA GGTATTGGAA GAGGGTGGGG AACAGTATTT TGATGCTCAG 
GAACCACAGA TTGATATGAA TGTAGATGGC TCTAAGAGAG AGAAGAACAT TGAAGAGGCA 
CAGTTCAGAT TATCAGAAAA TTACCTGATT CTGGATGGCT ATGAGAACGT GCAGGAGAGC 
TCCACAGATG ATGATCTCTC AGCCTCACAG TACCCCACCC AGTGCCCTGC TGTTAGTGAT 
CCAGCCGATC CTTTGCATGT GGAAGAGAGA CTGACTTTCA GTGCCCTCGA TCCTACACCA 
TCTTCTGAGG CTTTTAACCA CATGCTTCAG TACATCGCAA ACATTGAATC AGACTTGCAG 
CAGCTTAGGG GGCTTGAAGA GTTTTATAAC ACATATCGAA ACAGAGTGGC TACTTCAGGG 
GTCTTGGAGG AAGATCCAGA TAAGGCAATG CTTCCTACAG ATAAGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761047.1
CDS	779..5089
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATESSSRKTKSTWDESRQETYGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905187.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905187.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG 
GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT 
GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA 
GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT 
GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC 
TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT 
TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA 
GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT 
ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA 
TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC 
AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT 
TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA 
GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT 
GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG 
GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA 
GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC 
CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG 
GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA 
ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA 
GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA 
CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC 
TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC 
TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG 
GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA 
TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG 
AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT 
GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG 
AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT 
AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA 
TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC 
ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT 
CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT 
GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA 
CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGGT TTACTGACCT GGGAACACCA 
AAACCTTTCA GAAAGTTGGA TAGCCTGGTT GTAGGTGACA CTCAGAGTGA GGATGAGCAC 
CATGAGATTG ACAAGGAACA AGATCCACCC AACTGGCAGC AGCTTGTTAG TAAGGAAATA 
CTGCAGAAAC TTAAACCGCA CGAGATCAAG CAACAGGAAG TCATACACGA GCTGTTTTAC 
ACTGAAAGGG CACATGTACA GAAACTGGGT GTTTTGGACC AAGTGTTTCA TCAGCGCGTG 
ATCCGTGAGA ATCTCTTAAC ACCTGCAGAG ACCCGAAATG TTTTCTCCAA CTTAGAAGAA 
ATCTTACAGC TTCATGTGGC ACTTAATGAG CAAATGAAAG CAGTAAAGAA GCGTTATGAA 
ACATCTGTGG TGGAACAAAT TGGAGATGAT GTTCTGAACT GGTTCAGTGG AGCGGAAGAG 
GAGAAGCTAA AGCAGGCAGT GGCCACATTT TGCAGTAACC AGCCCTTTGC GTTGGAGATG 
ATAAAATCTC GCCAAAAGAA AGATCTAAAA TTCCTAACCT TTGTACAGGA TGCAGAGAGT 
AATCCTCTGT GTAAGCGCCT GCAGTTGAAG GATATTATTC CCACTGAGAT GCAGAGACTA 
ACCAAGTATC CACTGCTGCT GGATAACATT GCCAGATATT CAGATCGAGA AGATGAGAAA 
GAAAAGGTGA ACAGAGCAGC AAACCACTGC CGTAACATCT TGGACTACGT AAATCAGGCA 
GTTAAAGAGG CAGAAAACAA GCAGAGACTT GAAGATTATC AACGCAGGCT GGACCTGTCC 
TATTTGAAGC CAGGAGAGTA TCCCATGATA GACGAACTGA GAAATCTAGA TTTGACCAAG 
AGAAAATTGA TACATGAGGG GCCTCTGACA TGGAAGGTTA ATAGAGACAA ATCAATCGAT 
CTTTACTCTC TGCTGCTAGA GGACATTTTG GTTTTACTTC AGAGACAAGA TGACAAGTTG 
ATTTTGAGGT GTCACAGCAA GATTCTGGCT TCAACTTCAG ACAGCAAACA TATCTTCAGC 
CCTGTTATCA AACTCAACAC CGTGTTAGTA CGGCAGGTGG CAACAGATAA CAAGGCATTC 
TTTGTTATAT CCATGTCAAA AAATGGGGCA CAGATTTACG AGCTCATTGC CAACTCTGTT 
TCCGAGAAAA ATGGGTGGCA AGACCTCATA ACAAAAATGG CTGCAACATC GAAGACTGAA 
TATCCTCAAC CTACTCAGTT ACAACAGACT GCCCCAGAGA TGGTCAGCGA AGAAGAACAT 
GAAGATGTGG AGCAACAGAA AGTGTCACAT GATCAACAAG AAGATAGACT TATCTATAGG 
CACAGTACAG ACAAAGACTC AGACACCGAA AGTTTAGTTC AATCGAAATC CCCCAGCAGC 
CCCAGTCCCT CCTCCTGTCT TTCATCTACA GAGCATCACC TGGCAGTGAA CGCGCTCAGA 
AACTTGGGAC TGCTAAAACA GTTACTGGTG CAGCAGCTGA AGCTCCTTCA GAAAGAGGAA 
GCCGAGAACT TTCAACGATT TTGCAGCCGT AAAACAAAGG AGCAGATGAG GGAGAGTGAA 
AAACTTACTA AGTTTTCAAG CTTGGATAAG CAGAACTGGG GAAGCAGGGA GTCCGATTTG 
AACAAAGAAC TGCATGCTCA GCAAAGGGCC ACAGACAGAC GTTCTGAACA TAGCAACACT 
GGCCCAGACT GTGTTGCTGA GGTATTGGAA GAGGGTGGGG AACAGTATTT TGATGCTCAG 
GAACCACAGA TTGATATGAA TGTAGATGGC TCTAAGAGAG AGAAGAACAT TGAAGAGGCA 
CAGTTCAGAT TATCAGAAAA TTACCTGATT CTGGATGGCT ATGAGAACGT GCAGGAGAGC 
TCCACAGATG ATGATCTCTC AGCCTCACAG TACCCCACCC AGTGCCCTGC TGTTAGTGAT 
CCAGCCGATC CTTTGCATGT GGAAGAGAGA CTGACTTTCA GTGCCCTCGA TCCTACACCA 
TCTTCTGAGG CTTTTAACCA CATGCTTCAG TACATCGCAA ACATTGAATC AGACTTGCAG 
CAGCTTAGGG GGCTTGAAGA GTTTTATAAC ACATATCGAA ACAGAGTGGC TACTTCAGGG 
GTCTTGGAGG AAGATCCAGA TAAGGCAATG CTTCCTACAG ATAAGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_031905188.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761048.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa61525
Clone ID Related Accession (Same CDS sequence)	XM_031905188.1
Accession Version	XM_031905188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA 
CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC 
CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG 
GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT 
GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC 
TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT 
CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC 
CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA 
GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA 
AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA 
AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA 
CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT 
ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG 
TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC 
TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT 
CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA 
AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA 
GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA 
TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT 
GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC 
ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA 
TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG 
CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGCAGGTT TACTGACCTG 
GGAACACCAA AACCTTTCAG AAAGTTGGAT AGCCTGGTTG TAGGTGACAC TCAGAGTGAG 
GATGAGCACC ATGAGATTGA CAAGGAACAA GATCCACCCA ACTGGCAGCA GCTTGTTAGT 
AAGGAAATAC TGCAGAAACT TAAACCGCAC GAGATCAAGC AACAGGAAGT CATACACGAG 
CTGTTTTACA CTGAAAGGGC ACATGTACAG AAACTGGGTG TTTTGGACCA AGTGTTTCAT 
CAGCGCGTGA TCCGTGAGAA TCTCTTAACA CCTGCAGAGA CCCGAAATGT TTTCTCCAAC 
TTAGAAGAAA TCTTACAGCT TCATGTGGCA CTTAATGAGC AAATGAAAGC AGTAAAGAAG 
CGTTATGAAA CATCTGTGGT GGAACAAATT GGAGATGATG TTCTGAACTG GTTCAGTGGA 
GCGGAAGAGG AGAAGCTAAA GCAGGCAGTG GCCACATTTT GCAGTAACCA GCCCTTTGCG 
TTGGAGATGA TAAAATCTCG CCAAAAGAAA GATCTAAAAT TCCTAACCTT TGTACAGGAT 
GCAGAGAGTA ATCCTCTGTG TAAGCGCCTG CAGTTGAAGG ATATTATTCC CACTGAGATG 
CAGAGACTAA CCAAGTATCC ACTGCTGCTG GATAACATTG CCAGATATTC AGATCGAGAA 
GATGAGAAAG AAAAGGTGAA CAGAGCAGCA AACCACTGCC GTAACATCTT GGACTACGTA 
AATCAGGCAG TTAAAGAGGC AGAAAACAAG CAGAGACTTG AAGATTATCA ACGCAGGCTG 
GACCTGTCCT ATTTGAAGCC AGGAGAGTAT CCCATGATAG ACGAACTGAG AAATCTAGAT 
TTGACCAAGA GAAAATTGAT ACATGAGGGG CCTCTGACAT GGAAGGTTAA TAGAGACAAA 
TCAATCGATC TTTACTCTCT GCTGCTAGAG GACATTTTGG TTTTACTTCA GAGACAAGAT 
GACAAGTTGA TTTTGAGGTG TCACAGCAAG ATTCTGGCTT CAACTTCAGA CAGCAAACAT 
ATCTTCAGCC CTGTTATCAA ACTCAACACC GTGTTAGTAC GGCAGGTGGC AACAGATAAC 
AAGGCATTCT TTGTTATATC CATGTCAAAA AATGGGGCAC AGATTTACGA GCTCATTGCC 
AACTCTGTTT CCGAGAAAAA TGGGTGGCAA GACCTCATAA CAAAAATGGC TGCAACATCG 
AAGACTGAAT ATCCTCAACC TACTCAGTTA CAACAGACTG CCCCAGAGAT GGTCAGCGAA 
GAAGAACATG AAGATGTGGA GCAACAGAAA GTGTCACATG ATCAACAAGA AGATAGACTT 
ATCTATAGGC ACAGTACAGA CAAAGACTCA GACACCGAAA GTTTAGTTCA ATCGAAATCC 
CCCAGCAGCC CCAGTCCCTC CTCCTGTCTT TCATCTACAG AGCATCACCT GGCAGTGAAC 
GCGCTCAGAA ACTTGGGACT GCTAAAACAG TTACTGGTGC AGCAGCTGAA GCTCCTTCAG 
AAAGAGGAAG CCGAGAACTT TCAACGATTT TGCAGCCGTA AAACAAAGGA GCAGATGAGG 
GAGAGTGAAA AACTTACTAA GTTTTCAAGC TTGGATAAGC AGAACTGGGG AAGCAGGGAG 
TCCGATTTGA ACAAAGAACT GCATGCTCAG CAAAGGGCCA CAGACAGACG TTCTGAACAT 
AGCAACACTG GCCCAGACTG TGTTGCTGAG GTATTGGAAG AGGGTGGGGA ACAGTATTTT 
GATGCTCAGG AACCACAGAT TGATATGAAT GTAGATGGCT CTAAGAGAGA GAAGAACATT 
GAAGAGGCAC AGTTCAGATT ATCAGAAAAT TACCTGATTC TGGATGGCTA TGAGAACGTG 
CAGGAGAGCT CCACAGATGA TGATCTCTCA GCCTCACAGT ACCCCACCCA GTGCCCTGCT 
GTTAGTGATC CAGCCGATCC TTTGCATGTG GAAGAGAGAC TGACTTTCAG TGCCCTCGAT 
CCTACACCAT CTTCTGAGGC TTTTAACCAC ATGCTTCAGT ACATCGCAAA CATTGAATCA 
GACTTGCAGC AGCTTAGGGG GCTTGAAGAG TTTTATAACA CATATCGAAA CAGAGTGGCT 
ACTTCAGGGG TCTTGGAGGA AGATCCAGAT AAGGCAATGC TTCCTACAGA TAAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761048.1
CDS	779..5038
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADSRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905188.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905188.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA 
CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC 
CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG 
GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT 
GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC 
TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT 
CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC 
CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA 
GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA 
AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA 
AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA 
CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT 
ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG 
TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC 
TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT 
CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA 
AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA 
GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA 
TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT 
GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC 
ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA 
TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG 
CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGCAGGTT TACTGACCTG 
GGAACACCAA AACCTTTCAG AAAGTTGGAT AGCCTGGTTG TAGGTGACAC TCAGAGTGAG 
GATGAGCACC ATGAGATTGA CAAGGAACAA GATCCACCCA ACTGGCAGCA GCTTGTTAGT 
AAGGAAATAC TGCAGAAACT TAAACCGCAC GAGATCAAGC AACAGGAAGT CATACACGAG 
CTGTTTTACA CTGAAAGGGC ACATGTACAG AAACTGGGTG TTTTGGACCA AGTGTTTCAT 
CAGCGCGTGA TCCGTGAGAA TCTCTTAACA CCTGCAGAGA CCCGAAATGT TTTCTCCAAC 
TTAGAAGAAA TCTTACAGCT TCATGTGGCA CTTAATGAGC AAATGAAAGC AGTAAAGAAG 
CGTTATGAAA CATCTGTGGT GGAACAAATT GGAGATGATG TTCTGAACTG GTTCAGTGGA 
GCGGAAGAGG AGAAGCTAAA GCAGGCAGTG GCCACATTTT GCAGTAACCA GCCCTTTGCG 
TTGGAGATGA TAAAATCTCG CCAAAAGAAA GATCTAAAAT TCCTAACCTT TGTACAGGAT 
GCAGAGAGTA ATCCTCTGTG TAAGCGCCTG CAGTTGAAGG ATATTATTCC CACTGAGATG 
CAGAGACTAA CCAAGTATCC ACTGCTGCTG GATAACATTG CCAGATATTC AGATCGAGAA 
GATGAGAAAG AAAAGGTGAA CAGAGCAGCA AACCACTGCC GTAACATCTT GGACTACGTA 
AATCAGGCAG TTAAAGAGGC AGAAAACAAG CAGAGACTTG AAGATTATCA ACGCAGGCTG 
GACCTGTCCT ATTTGAAGCC AGGAGAGTAT CCCATGATAG ACGAACTGAG AAATCTAGAT 
TTGACCAAGA GAAAATTGAT ACATGAGGGG CCTCTGACAT GGAAGGTTAA TAGAGACAAA 
TCAATCGATC TTTACTCTCT GCTGCTAGAG GACATTTTGG TTTTACTTCA GAGACAAGAT 
GACAAGTTGA TTTTGAGGTG TCACAGCAAG ATTCTGGCTT CAACTTCAGA CAGCAAACAT 
ATCTTCAGCC CTGTTATCAA ACTCAACACC GTGTTAGTAC GGCAGGTGGC AACAGATAAC 
AAGGCATTCT TTGTTATATC CATGTCAAAA AATGGGGCAC AGATTTACGA GCTCATTGCC 
AACTCTGTTT CCGAGAAAAA TGGGTGGCAA GACCTCATAA CAAAAATGGC TGCAACATCG 
AAGACTGAAT ATCCTCAACC TACTCAGTTA CAACAGACTG CCCCAGAGAT GGTCAGCGAA 
GAAGAACATG AAGATGTGGA GCAACAGAAA GTGTCACATG ATCAACAAGA AGATAGACTT 
ATCTATAGGC ACAGTACAGA CAAAGACTCA GACACCGAAA GTTTAGTTCA ATCGAAATCC 
CCCAGCAGCC CCAGTCCCTC CTCCTGTCTT TCATCTACAG AGCATCACCT GGCAGTGAAC 
GCGCTCAGAA ACTTGGGACT GCTAAAACAG TTACTGGTGC AGCAGCTGAA GCTCCTTCAG 
AAAGAGGAAG CCGAGAACTT TCAACGATTT TGCAGCCGTA AAACAAAGGA GCAGATGAGG 
GAGAGTGAAA AACTTACTAA GTTTTCAAGC TTGGATAAGC AGAACTGGGG AAGCAGGGAG 
TCCGATTTGA ACAAAGAACT GCATGCTCAG CAAAGGGCCA CAGACAGACG TTCTGAACAT 
AGCAACACTG GCCCAGACTG TGTTGCTGAG GTATTGGAAG AGGGTGGGGA ACAGTATTTT 
GATGCTCAGG AACCACAGAT TGATATGAAT GTAGATGGCT CTAAGAGAGA GAAGAACATT 
GAAGAGGCAC AGTTCAGATT ATCAGAAAAT TACCTGATTC TGGATGGCTA TGAGAACGTG 
CAGGAGAGCT CCACAGATGA TGATCTCTCA GCCTCACAGT ACCCCACCCA GTGCCCTGCT 
GTTAGTGATC CAGCCGATCC TTTGCATGTG GAAGAGAGAC TGACTTTCAG TGCCCTCGAT 
CCTACACCAT CTTCTGAGGC TTTTAACCAC ATGCTTCAGT ACATCGCAAA CATTGAATCA 
GACTTGCAGC AGCTTAGGGG GCTTGAAGAG TTTTATAACA CATATCGAAA CAGAGTGGCT 
ACTTCAGGGG TCTTGGAGGA AGATCCAGAT AAGGCAATGC TTCCTACAGA TAAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_031905189.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761049.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa61526
Clone ID Related Accession (Same CDS sequence)	XM_031905189.1
Accession Version	XM_031905189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA 
CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC 
CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG 
GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT 
GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC 
TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT 
CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC 
CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA 
GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA 
AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA 
AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA 
CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT 
ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG 
TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC 
TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT 
CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA 
AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA 
GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA 
TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT 
GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC 
ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA 
TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG 
CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGGTTTAC TGACCTGGGA 
ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT 
GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG 
GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG 
TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG 
CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA 
GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT 
TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG 
GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG 
GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA 
GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG 
AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT 
GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT 
CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC 
CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG 
ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA 
ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC 
AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC 
TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG 
GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC 
TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG 
ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA 
GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC 
TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC 
AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG 
CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA 
GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG 
AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC 
GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC 
AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT 
GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA 
GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG 
GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT 
AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT 
ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC 
TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT 
TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761049.1
CDS	779..5035
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	XM_031905189.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905189.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA 
CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC 
CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG 
GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT 
GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC 
TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT 
CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC 
CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA 
GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA 
AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA 
AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA 
CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT 
ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG 
TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC 
TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT 
CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA 
AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA 
GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA 
TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT 
GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC 
ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA 
TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG 
CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGGTTTAC TGACCTGGGA 
ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT 
GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG 
GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG 
TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG 
CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA 
GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT 
TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG 
GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG 
GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA 
GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG 
AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT 
GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT 
CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC 
CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG 
ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA 
ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC 
AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC 
TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG 
GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC 
TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG 
ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA 
GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC 
TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC 
AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG 
CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA 
GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG 
AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC 
GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC 
AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT 
GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA 
GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG 
GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT 
AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT 
ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC 
TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT 
TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( NM_001079028.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072496.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), mRNA.

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CloneID	OXa22766
Clone ID Related Accession (Same CDS sequence)	NM_001079028.1
Accession Version	NM_001079028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121927.1. ##Evidence-Data-START## Transcript exon combination :: BC121927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCTGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAA GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCGACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072496.1
CDS	109..4362
Translation	MSGTQSAASDRFHSRKPTRHGSILYRESSTDKKQKVERSVSSSHDFDATGLVQRCVVIQRDENGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHIEVVKLIKSGSYVALTVQGRPPGSPTIPLTDSEADVHNLGPIMPPHSPVTQGHGKGITSPILMEEENNIVHTQKLDILKKMLQKEQDQLQQFQEEYNSTPSPRLSKEIQDSKKHIAQLQERLLKARGLSQGSESLQISELDADSSDILGRTDYSSGDGSRPNSDIADSPRSGQREKSFLEESPEKSEIQDTDSQSSMGSPSIRFTHNIIGAEDDDFDAEPEQQMNGQCSCFQSMDLLKSHPAHLAVFIHHVVSQFDPAAMLCYLYTDLYQQTNSKETRRVFLEFNQFFLEKAGNLRVSVPDDVALELEKRRPELIPEELHRQYIQTMQERLSEDVKKNLEDFKQKRSMGLTLAEGELSKLDADRLRDRNTMEKERVCAEQILQKIDEILFSSQTPEDEKSNTIQYVILTYMKHLGVKVKEPRSLEQKRGRIGFLPKIKQSMKKDREGEEKVKRRGFPSILAPQRKPSRQDSNAIGRAMELQRPRHPKHLSTSSSSSPEPADGSRLRTSGGSIEGSDTPFSPSSNLGAAYTFQEVGREVEGNFRCGENMSSADTLDGGTRTSGPIFDFPSPPLDHLHEETGEADRFTDLGTPKPFRKLDSLVVGDTQSEDEHHEIDKEQDPPNWQQLVSKEILQKLKPHEIKQQEVIHELFYTERAHVQKLGVLDQVFHQRVIRENLLTPAETRNVFSNLEEILQLHVALNEQMKAVKKRYETSVVEQIGDDVLNWFSGAEEEKLKQAVATFCSNQPFALEMIKSRQKKDLKFLTFVQDAESNPLCKRLQLKDIIPTEMQRLTKYPLLLDNIARYSDREDEKEKVNRAANHCRNILDYVNQAVKEAENKQRLEDYQRRLDLSYLKPGEYPMIDELRNLDLTKRKLIHEGPLTWKVNRDKSIDLYSLLLEDILVLLQRQDDKLILRCHSKILASTSDSKHIFSPVIKLNTVLVRQVATDNKAFFVISMSKNGAQIYELIANSVSEKNGWQDLITKMAATSKTEYPQPTQLQQTAPEMVSEEEHEDVEQQKVSHDQQEDRLIYRHSTDKDSDTESLVQSKSPSSPSPSSCLSSTEHHLAVNALRNLGLLKQLLVQQLKLLQKEEAENFQRFCSRKTKEQMRESEKLTKFSSLDKQNWGSRESDLNKELHAQQRATDRRSEHSNTGPDCVAEVLEEGGEQYFDAQEPQIDMNVDGSKREKNIEEAQFRLSENYLILDGYENVQESSTDDDLSASQYPTQCPAVSDPADPLHVEERLTFSALDPTPSSEAFNHMLQYIANIESDLQQLRGLEEFYNTYRNRVATSGVLEEDPDKAMLPTDKG

Target ORF information:

RefSeq Version	NM_001079028.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079028.1

ORF Insert Sequence:

ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT 
AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT 
GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC 
AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT 
AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCTGGC 
TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT 
GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC 
CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG 
CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA 
CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC 
AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG 
GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC 
ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT 
AGTGGACAGC GAGAGAAAAG CTTCCTGGAA GAAAGTCCTG AGAAAAGTGA GATTCAGGAC 
ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT 
GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT 
TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT 
GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG 
CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA 
AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG 
AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG 
CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG 
ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA 
ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT 
TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT 
ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA 
ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG 
GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT 
AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC 
ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC 
TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC 
TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA 
GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA 
CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA 
CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG 
CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA 
ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT 
TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC 
GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA 
GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT 
GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA 
GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG 
ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG 
AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA 
CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG 
AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG 
GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG 
TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC 
AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC 
GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG 
TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCGACTT CAGACAGCAA ACATATCTTC 
AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA 
TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT 
GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT 
GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA 
CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT 
AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC 
AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC 
AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG 
GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT 
GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT 
TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC 
ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT 
CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG 
GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG 
AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT 
GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA 
CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG 
CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA 
GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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オンライン注文

arhgef12 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム