arhgef12 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following arhgef12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa61521 XM_031905184.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61522 XM_031905185.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61523 XM_031905186.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61524 XM_031905187.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61525 XM_031905188.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61526 XM_031905189.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa22766 NM_001079028.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa61521
    Clone ID Related Accession (Same CDS sequence) XM_031905184.1
    Accession Version XM_031905184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GCAGGTTTAC TGACCTGGGA
    ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT
    GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG
    GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG
    TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG
    CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA
    GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT
    TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG
    GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG
    GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA
    GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG
    AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT
    GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT
    CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC
    CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG
    ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA
    ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC
    AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC
    TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG
    GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC
    TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG
    ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA
    GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC
    TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC
    AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG
    CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA
    GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG
    AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC
    GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC
    AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT
    GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA
    GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG
    GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT
    AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT
    ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC
    TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT
    TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761044.1
    CDS779..5095
    Translation

    Target ORF information:

    RefSeq Version XM_031905184.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GCAGGTTTAC TGACCTGGGA
    ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT
    GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG
    GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG
    TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG
    CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA
    GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT
    TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG
    GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG
    GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA
    GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG
    AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT
    GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT
    CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC
    CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG
    ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA
    ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC
    AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC
    TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG
    GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC
    TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG
    ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA
    GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC
    TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC
    AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG
    CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA
    GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG
    AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC
    GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC
    AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT
    GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA
    GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG
    GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT
    AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT
    ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC
    TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT
    TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61522
    Clone ID Related Accession (Same CDS sequence) XM_031905185.1
    Accession Version XM_031905185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT
    TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA
    GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT
    GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG
    GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA
    GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC
    CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG
    GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA
    ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA
    GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA
    CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC
    TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC
    TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG
    GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA
    TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG
    AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT
    GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG
    AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT
    AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA
    TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC
    ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT
    CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT
    GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA
    CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGCA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761045.1
    CDS779..5092
    Translation

    Target ORF information:

    RefSeq Version XM_031905185.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT
    TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA
    GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT
    GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG
    GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA
    GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC
    CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG
    GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA
    ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA
    GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA
    CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC
    TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC
    TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG
    GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA
    TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG
    AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT
    GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG
    AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT
    AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA
    TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC
    ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT
    CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT
    GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA
    CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGCA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61523
    Clone ID Related Accession (Same CDS sequence) XM_031905186.1
    Accession Version XM_031905186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761046.1
    CDS779..5092
    Translation

    Target ORF information:

    RefSeq Version XM_031905186.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGCAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAG GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCAACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61524
    Clone ID Related Accession (Same CDS sequence) XM_031905187.1
    Accession Version XM_031905187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT
    TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA
    GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT
    GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG
    GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA
    GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC
    CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG
    GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA
    ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA
    GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA
    CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC
    TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC
    TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG
    GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA
    TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG
    AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT
    GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG
    AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT
    AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA
    TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC
    ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT
    CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT
    GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA
    CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGGT TTACTGACCT GGGAACACCA
    AAACCTTTCA GAAAGTTGGA TAGCCTGGTT GTAGGTGACA CTCAGAGTGA GGATGAGCAC
    CATGAGATTG ACAAGGAACA AGATCCACCC AACTGGCAGC AGCTTGTTAG TAAGGAAATA
    CTGCAGAAAC TTAAACCGCA CGAGATCAAG CAACAGGAAG TCATACACGA GCTGTTTTAC
    ACTGAAAGGG CACATGTACA GAAACTGGGT GTTTTGGACC AAGTGTTTCA TCAGCGCGTG
    ATCCGTGAGA ATCTCTTAAC ACCTGCAGAG ACCCGAAATG TTTTCTCCAA CTTAGAAGAA
    ATCTTACAGC TTCATGTGGC ACTTAATGAG CAAATGAAAG CAGTAAAGAA GCGTTATGAA
    ACATCTGTGG TGGAACAAAT TGGAGATGAT GTTCTGAACT GGTTCAGTGG AGCGGAAGAG
    GAGAAGCTAA AGCAGGCAGT GGCCACATTT TGCAGTAACC AGCCCTTTGC GTTGGAGATG
    ATAAAATCTC GCCAAAAGAA AGATCTAAAA TTCCTAACCT TTGTACAGGA TGCAGAGAGT
    AATCCTCTGT GTAAGCGCCT GCAGTTGAAG GATATTATTC CCACTGAGAT GCAGAGACTA
    ACCAAGTATC CACTGCTGCT GGATAACATT GCCAGATATT CAGATCGAGA AGATGAGAAA
    GAAAAGGTGA ACAGAGCAGC AAACCACTGC CGTAACATCT TGGACTACGT AAATCAGGCA
    GTTAAAGAGG CAGAAAACAA GCAGAGACTT GAAGATTATC AACGCAGGCT GGACCTGTCC
    TATTTGAAGC CAGGAGAGTA TCCCATGATA GACGAACTGA GAAATCTAGA TTTGACCAAG
    AGAAAATTGA TACATGAGGG GCCTCTGACA TGGAAGGTTA ATAGAGACAA ATCAATCGAT
    CTTTACTCTC TGCTGCTAGA GGACATTTTG GTTTTACTTC AGAGACAAGA TGACAAGTTG
    ATTTTGAGGT GTCACAGCAA GATTCTGGCT TCAACTTCAG ACAGCAAACA TATCTTCAGC
    CCTGTTATCA AACTCAACAC CGTGTTAGTA CGGCAGGTGG CAACAGATAA CAAGGCATTC
    TTTGTTATAT CCATGTCAAA AAATGGGGCA CAGATTTACG AGCTCATTGC CAACTCTGTT
    TCCGAGAAAA ATGGGTGGCA AGACCTCATA ACAAAAATGG CTGCAACATC GAAGACTGAA
    TATCCTCAAC CTACTCAGTT ACAACAGACT GCCCCAGAGA TGGTCAGCGA AGAAGAACAT
    GAAGATGTGG AGCAACAGAA AGTGTCACAT GATCAACAAG AAGATAGACT TATCTATAGG
    CACAGTACAG ACAAAGACTC AGACACCGAA AGTTTAGTTC AATCGAAATC CCCCAGCAGC
    CCCAGTCCCT CCTCCTGTCT TTCATCTACA GAGCATCACC TGGCAGTGAA CGCGCTCAGA
    AACTTGGGAC TGCTAAAACA GTTACTGGTG CAGCAGCTGA AGCTCCTTCA GAAAGAGGAA
    GCCGAGAACT TTCAACGATT TTGCAGCCGT AAAACAAAGG AGCAGATGAG GGAGAGTGAA
    AAACTTACTA AGTTTTCAAG CTTGGATAAG CAGAACTGGG GAAGCAGGGA GTCCGATTTG
    AACAAAGAAC TGCATGCTCA GCAAAGGGCC ACAGACAGAC GTTCTGAACA TAGCAACACT
    GGCCCAGACT GTGTTGCTGA GGTATTGGAA GAGGGTGGGG AACAGTATTT TGATGCTCAG
    GAACCACAGA TTGATATGAA TGTAGATGGC TCTAAGAGAG AGAAGAACAT TGAAGAGGCA
    CAGTTCAGAT TATCAGAAAA TTACCTGATT CTGGATGGCT ATGAGAACGT GCAGGAGAGC
    TCCACAGATG ATGATCTCTC AGCCTCACAG TACCCCACCC AGTGCCCTGC TGTTAGTGAT
    CCAGCCGATC CTTTGCATGT GGAAGAGAGA CTGACTTTCA GTGCCCTCGA TCCTACACCA
    TCTTCTGAGG CTTTTAACCA CATGCTTCAG TACATCGCAA ACATTGAATC AGACTTGCAG
    CAGCTTAGGG GGCTTGAAGA GTTTTATAAC ACATATCGAA ACAGAGTGGC TACTTCAGGG
    GTCTTGGAGG AAGATCCAGA TAAGGCAATG CTTCCTACAG ATAAGGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761047.1
    CDS779..5089
    Translation

    Target ORF information:

    RefSeq Version XM_031905187.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGAG AGCTCGTCCA GGAAGACAAA GTCTACTTGG
    GATGAGAGTA GACAAGAGAC TTATGGTTTA GTTCAGCGCT GTGTGGTTAT CCAACGTGAT
    GAAAATGGCT TTGGCCTGAC AGTCAGTGGC GATAACCCTG TCTTTGTTCA GTCTGTCAAA
    GAAGATGGCG CTGCCATGCG AGCTGGAGTT CAGACTGGGG ATCGGATCAT CAAGGTTAAT
    GGCACTTTGG TCACACACTC CAATCATATA GAAGTGGTCA AACTCATAAA GTCGGGCTCC
    TATGTGGCTC TTACAGTACA AGGTCGTCCA CCTGGGTCAC CCACCATCCC TTTAACTGAT
    TCTGAGGCAG ATGTGCACAA TTTAGGTCCC ATAATGCCTC CTCATTCTCC AGTAACCCAA
    GGCCATGGAA AAGGTATTAC AAGCCCTATA CTTATGGAGG AGGAAAATAA CATTGTGCAT
    ACTCAGAAAC TGGACATATT GAAGAAGATG CTCCAGAAAG AACAAGACCA GCTACAACAA
    TTTCAAGAAG AGTATAACTC TACTCCATCA CCAAGGCTTT CTAAGGAGAT CCAAGACAGC
    AAAAAACACA TAGCCCAGTT GCAGGAGCGA TTGTTGAAAG CCAGAGGTCT TTCACAGGGT
    TCTGAATCAC TGCAGATCAG TGAGCTTGAT GCAGACTCTA GCGACATACT GGGACGCACA
    GATTACAGCA GTGGGGATGG ATCACGACCA AACAGTGACA TTGCAGATAG CCCCCGTAGT
    GGACAGCGAG AGAAAAGCTT CCTGGAGGAA AGTCCTGAGA AAAGTGAGAT TCAGGACACG
    GACTCACAGT CAAGTATGGG AAGCCCCTCT ATTCGCTTCA CTCATAATAT TATTGGTGCA
    GAGGATGATG ACTTTGATGC CGAACCAGAG CAGCAGATGA ATGGGCAGTG TAGCTGTTTC
    CAGAGCATGG ACCTGTTAAA ATCACACCCA GCTCACCTAG CAGTATTTAT ACATCATGTG
    GTCTCCCAAT TTGATCCAGC AGCCATGCTG TGCTACCTCT ATACAGACTT GTACCAGCAA
    ACAAATTCTA AAGAGACTCG CAGGGTGTTT CTGGAGTTCA ACCAATTCTT CTTAGAAAAA
    GCCGGGAATC TTAGAGTTTC TGTCCCAGAT GATGTTGCTC TTGAATTAGA GAAAAGGAGA
    CCAGAGCTGA TTCCAGAGGA GCTACATCGA CAATACATCC AGACTATGCA AGAAAGGCTC
    TCTGAGGATG TTAAAAAAAA CTTGGAGGAT TTTAAGCAAA AGCGCAGCAT GGGACTGACC
    TTGGCAGAGG GAGAGCTCTC CAAACTGGAT GCAGATCGAT TGAGAGACCG CAATACAATG
    GAGAAGGAAA GAGTCTGTGC AGAGCAGATT CTTCAGAAGA TTGATGAAAT TCTGTTTTCA
    TCACAGACTC CTGAAGATGA AAAGAGCAAT ACCATACAAT ATGTGATTCT AACCTATATG
    AAGCATCTTG GTGTGAAAGT TAAAGAGCCA CGAAGTCTTG AACAGAAACG TGGTCGAATT
    GGATTCCTCC CAAAAATTAA GCAAAGTATG AAGAAAGATC GGGAGGGTGA GGAAAAGGTG
    AAGCGAAGAG GGTTTCCCAG CATCCTTGCA CCTCAGAGGA AGCCTAGCCG ACAAGATAGT
    AATGCAATTG GCAGGGCTAT GGAACTTCAG CGGCCACGCC ACCCCAAGCA CTTATCCACA
    TCTTCATCCA GTAGCCCTGA ACCAGCAGAT GGAAGTAGAT TGCGCACTAG CGGTGGCTCC
    ATTGAGGGTT CTGACACACC GTTCTCTCCA TCATCTAATT TGGGTGCAGC ATACACCTTT
    CAGGAAGTTG GCAGAGAAGT GGAAGGAAAT TTTAGGTGTG GTGAAAATAT GTCATCAGCT
    GATACCCTTG ATGGTGGTAC TCGCACATCT GGCCCAATAT TTGACTTTCC ATCGCCACCA
    CTTGACCACC TACATGAAGA GACAGGTGAA GCAGACAGGT TTACTGACCT GGGAACACCA
    AAACCTTTCA GAAAGTTGGA TAGCCTGGTT GTAGGTGACA CTCAGAGTGA GGATGAGCAC
    CATGAGATTG ACAAGGAACA AGATCCACCC AACTGGCAGC AGCTTGTTAG TAAGGAAATA
    CTGCAGAAAC TTAAACCGCA CGAGATCAAG CAACAGGAAG TCATACACGA GCTGTTTTAC
    ACTGAAAGGG CACATGTACA GAAACTGGGT GTTTTGGACC AAGTGTTTCA TCAGCGCGTG
    ATCCGTGAGA ATCTCTTAAC ACCTGCAGAG ACCCGAAATG TTTTCTCCAA CTTAGAAGAA
    ATCTTACAGC TTCATGTGGC ACTTAATGAG CAAATGAAAG CAGTAAAGAA GCGTTATGAA
    ACATCTGTGG TGGAACAAAT TGGAGATGAT GTTCTGAACT GGTTCAGTGG AGCGGAAGAG
    GAGAAGCTAA AGCAGGCAGT GGCCACATTT TGCAGTAACC AGCCCTTTGC GTTGGAGATG
    ATAAAATCTC GCCAAAAGAA AGATCTAAAA TTCCTAACCT TTGTACAGGA TGCAGAGAGT
    AATCCTCTGT GTAAGCGCCT GCAGTTGAAG GATATTATTC CCACTGAGAT GCAGAGACTA
    ACCAAGTATC CACTGCTGCT GGATAACATT GCCAGATATT CAGATCGAGA AGATGAGAAA
    GAAAAGGTGA ACAGAGCAGC AAACCACTGC CGTAACATCT TGGACTACGT AAATCAGGCA
    GTTAAAGAGG CAGAAAACAA GCAGAGACTT GAAGATTATC AACGCAGGCT GGACCTGTCC
    TATTTGAAGC CAGGAGAGTA TCCCATGATA GACGAACTGA GAAATCTAGA TTTGACCAAG
    AGAAAATTGA TACATGAGGG GCCTCTGACA TGGAAGGTTA ATAGAGACAA ATCAATCGAT
    CTTTACTCTC TGCTGCTAGA GGACATTTTG GTTTTACTTC AGAGACAAGA TGACAAGTTG
    ATTTTGAGGT GTCACAGCAA GATTCTGGCT TCAACTTCAG ACAGCAAACA TATCTTCAGC
    CCTGTTATCA AACTCAACAC CGTGTTAGTA CGGCAGGTGG CAACAGATAA CAAGGCATTC
    TTTGTTATAT CCATGTCAAA AAATGGGGCA CAGATTTACG AGCTCATTGC CAACTCTGTT
    TCCGAGAAAA ATGGGTGGCA AGACCTCATA ACAAAAATGG CTGCAACATC GAAGACTGAA
    TATCCTCAAC CTACTCAGTT ACAACAGACT GCCCCAGAGA TGGTCAGCGA AGAAGAACAT
    GAAGATGTGG AGCAACAGAA AGTGTCACAT GATCAACAAG AAGATAGACT TATCTATAGG
    CACAGTACAG ACAAAGACTC AGACACCGAA AGTTTAGTTC AATCGAAATC CCCCAGCAGC
    CCCAGTCCCT CCTCCTGTCT TTCATCTACA GAGCATCACC TGGCAGTGAA CGCGCTCAGA
    AACTTGGGAC TGCTAAAACA GTTACTGGTG CAGCAGCTGA AGCTCCTTCA GAAAGAGGAA
    GCCGAGAACT TTCAACGATT TTGCAGCCGT AAAACAAAGG AGCAGATGAG GGAGAGTGAA
    AAACTTACTA AGTTTTCAAG CTTGGATAAG CAGAACTGGG GAAGCAGGGA GTCCGATTTG
    AACAAAGAAC TGCATGCTCA GCAAAGGGCC ACAGACAGAC GTTCTGAACA TAGCAACACT
    GGCCCAGACT GTGTTGCTGA GGTATTGGAA GAGGGTGGGG AACAGTATTT TGATGCTCAG
    GAACCACAGA TTGATATGAA TGTAGATGGC TCTAAGAGAG AGAAGAACAT TGAAGAGGCA
    CAGTTCAGAT TATCAGAAAA TTACCTGATT CTGGATGGCT ATGAGAACGT GCAGGAGAGC
    TCCACAGATG ATGATCTCTC AGCCTCACAG TACCCCACCC AGTGCCCTGC TGTTAGTGAT
    CCAGCCGATC CTTTGCATGT GGAAGAGAGA CTGACTTTCA GTGCCCTCGA TCCTACACCA
    TCTTCTGAGG CTTTTAACCA CATGCTTCAG TACATCGCAA ACATTGAATC AGACTTGCAG
    CAGCTTAGGG GGCTTGAAGA GTTTTATAAC ACATATCGAA ACAGAGTGGC TACTTCAGGG
    GTCTTGGAGG AAGATCCAGA TAAGGCAATG CTTCCTACAG ATAAGGGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61525
    Clone ID Related Accession (Same CDS sequence) XM_031905188.1
    Accession Version XM_031905188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA
    CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC
    CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG
    GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT
    GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC
    TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT
    CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC
    CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA
    GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA
    AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA
    AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA
    CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT
    ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG
    TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC
    TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT
    CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA
    AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA
    GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA
    TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT
    GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC
    ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA
    TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG
    CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGCAGGTT TACTGACCTG
    GGAACACCAA AACCTTTCAG AAAGTTGGAT AGCCTGGTTG TAGGTGACAC TCAGAGTGAG
    GATGAGCACC ATGAGATTGA CAAGGAACAA GATCCACCCA ACTGGCAGCA GCTTGTTAGT
    AAGGAAATAC TGCAGAAACT TAAACCGCAC GAGATCAAGC AACAGGAAGT CATACACGAG
    CTGTTTTACA CTGAAAGGGC ACATGTACAG AAACTGGGTG TTTTGGACCA AGTGTTTCAT
    CAGCGCGTGA TCCGTGAGAA TCTCTTAACA CCTGCAGAGA CCCGAAATGT TTTCTCCAAC
    TTAGAAGAAA TCTTACAGCT TCATGTGGCA CTTAATGAGC AAATGAAAGC AGTAAAGAAG
    CGTTATGAAA CATCTGTGGT GGAACAAATT GGAGATGATG TTCTGAACTG GTTCAGTGGA
    GCGGAAGAGG AGAAGCTAAA GCAGGCAGTG GCCACATTTT GCAGTAACCA GCCCTTTGCG
    TTGGAGATGA TAAAATCTCG CCAAAAGAAA GATCTAAAAT TCCTAACCTT TGTACAGGAT
    GCAGAGAGTA ATCCTCTGTG TAAGCGCCTG CAGTTGAAGG ATATTATTCC CACTGAGATG
    CAGAGACTAA CCAAGTATCC ACTGCTGCTG GATAACATTG CCAGATATTC AGATCGAGAA
    GATGAGAAAG AAAAGGTGAA CAGAGCAGCA AACCACTGCC GTAACATCTT GGACTACGTA
    AATCAGGCAG TTAAAGAGGC AGAAAACAAG CAGAGACTTG AAGATTATCA ACGCAGGCTG
    GACCTGTCCT ATTTGAAGCC AGGAGAGTAT CCCATGATAG ACGAACTGAG AAATCTAGAT
    TTGACCAAGA GAAAATTGAT ACATGAGGGG CCTCTGACAT GGAAGGTTAA TAGAGACAAA
    TCAATCGATC TTTACTCTCT GCTGCTAGAG GACATTTTGG TTTTACTTCA GAGACAAGAT
    GACAAGTTGA TTTTGAGGTG TCACAGCAAG ATTCTGGCTT CAACTTCAGA CAGCAAACAT
    ATCTTCAGCC CTGTTATCAA ACTCAACACC GTGTTAGTAC GGCAGGTGGC AACAGATAAC
    AAGGCATTCT TTGTTATATC CATGTCAAAA AATGGGGCAC AGATTTACGA GCTCATTGCC
    AACTCTGTTT CCGAGAAAAA TGGGTGGCAA GACCTCATAA CAAAAATGGC TGCAACATCG
    AAGACTGAAT ATCCTCAACC TACTCAGTTA CAACAGACTG CCCCAGAGAT GGTCAGCGAA
    GAAGAACATG AAGATGTGGA GCAACAGAAA GTGTCACATG ATCAACAAGA AGATAGACTT
    ATCTATAGGC ACAGTACAGA CAAAGACTCA GACACCGAAA GTTTAGTTCA ATCGAAATCC
    CCCAGCAGCC CCAGTCCCTC CTCCTGTCTT TCATCTACAG AGCATCACCT GGCAGTGAAC
    GCGCTCAGAA ACTTGGGACT GCTAAAACAG TTACTGGTGC AGCAGCTGAA GCTCCTTCAG
    AAAGAGGAAG CCGAGAACTT TCAACGATTT TGCAGCCGTA AAACAAAGGA GCAGATGAGG
    GAGAGTGAAA AACTTACTAA GTTTTCAAGC TTGGATAAGC AGAACTGGGG AAGCAGGGAG
    TCCGATTTGA ACAAAGAACT GCATGCTCAG CAAAGGGCCA CAGACAGACG TTCTGAACAT
    AGCAACACTG GCCCAGACTG TGTTGCTGAG GTATTGGAAG AGGGTGGGGA ACAGTATTTT
    GATGCTCAGG AACCACAGAT TGATATGAAT GTAGATGGCT CTAAGAGAGA GAAGAACATT
    GAAGAGGCAC AGTTCAGATT ATCAGAAAAT TACCTGATTC TGGATGGCTA TGAGAACGTG
    CAGGAGAGCT CCACAGATGA TGATCTCTCA GCCTCACAGT ACCCCACCCA GTGCCCTGCT
    GTTAGTGATC CAGCCGATCC TTTGCATGTG GAAGAGAGAC TGACTTTCAG TGCCCTCGAT
    CCTACACCAT CTTCTGAGGC TTTTAACCAC ATGCTTCAGT ACATCGCAAA CATTGAATCA
    GACTTGCAGC AGCTTAGGGG GCTTGAAGAG TTTTATAACA CATATCGAAA CAGAGTGGCT
    ACTTCAGGGG TCTTGGAGGA AGATCCAGAT AAGGCAATGC TTCCTACAGA TAAGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761048.1
    CDS779..5038
    Translation

    Target ORF information:

    RefSeq Version XM_031905188.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA
    CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC
    CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG
    GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT
    GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC
    TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT
    CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC
    CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA
    GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA
    AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA
    AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA
    CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT
    ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG
    TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC
    TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT
    CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA
    AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA
    GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA
    TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT
    GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC
    ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA
    TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG
    CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGCAGGTT TACTGACCTG
    GGAACACCAA AACCTTTCAG AAAGTTGGAT AGCCTGGTTG TAGGTGACAC TCAGAGTGAG
    GATGAGCACC ATGAGATTGA CAAGGAACAA GATCCACCCA ACTGGCAGCA GCTTGTTAGT
    AAGGAAATAC TGCAGAAACT TAAACCGCAC GAGATCAAGC AACAGGAAGT CATACACGAG
    CTGTTTTACA CTGAAAGGGC ACATGTACAG AAACTGGGTG TTTTGGACCA AGTGTTTCAT
    CAGCGCGTGA TCCGTGAGAA TCTCTTAACA CCTGCAGAGA CCCGAAATGT TTTCTCCAAC
    TTAGAAGAAA TCTTACAGCT TCATGTGGCA CTTAATGAGC AAATGAAAGC AGTAAAGAAG
    CGTTATGAAA CATCTGTGGT GGAACAAATT GGAGATGATG TTCTGAACTG GTTCAGTGGA
    GCGGAAGAGG AGAAGCTAAA GCAGGCAGTG GCCACATTTT GCAGTAACCA GCCCTTTGCG
    TTGGAGATGA TAAAATCTCG CCAAAAGAAA GATCTAAAAT TCCTAACCTT TGTACAGGAT
    GCAGAGAGTA ATCCTCTGTG TAAGCGCCTG CAGTTGAAGG ATATTATTCC CACTGAGATG
    CAGAGACTAA CCAAGTATCC ACTGCTGCTG GATAACATTG CCAGATATTC AGATCGAGAA
    GATGAGAAAG AAAAGGTGAA CAGAGCAGCA AACCACTGCC GTAACATCTT GGACTACGTA
    AATCAGGCAG TTAAAGAGGC AGAAAACAAG CAGAGACTTG AAGATTATCA ACGCAGGCTG
    GACCTGTCCT ATTTGAAGCC AGGAGAGTAT CCCATGATAG ACGAACTGAG AAATCTAGAT
    TTGACCAAGA GAAAATTGAT ACATGAGGGG CCTCTGACAT GGAAGGTTAA TAGAGACAAA
    TCAATCGATC TTTACTCTCT GCTGCTAGAG GACATTTTGG TTTTACTTCA GAGACAAGAT
    GACAAGTTGA TTTTGAGGTG TCACAGCAAG ATTCTGGCTT CAACTTCAGA CAGCAAACAT
    ATCTTCAGCC CTGTTATCAA ACTCAACACC GTGTTAGTAC GGCAGGTGGC AACAGATAAC
    AAGGCATTCT TTGTTATATC CATGTCAAAA AATGGGGCAC AGATTTACGA GCTCATTGCC
    AACTCTGTTT CCGAGAAAAA TGGGTGGCAA GACCTCATAA CAAAAATGGC TGCAACATCG
    AAGACTGAAT ATCCTCAACC TACTCAGTTA CAACAGACTG CCCCAGAGAT GGTCAGCGAA
    GAAGAACATG AAGATGTGGA GCAACAGAAA GTGTCACATG ATCAACAAGA AGATAGACTT
    ATCTATAGGC ACAGTACAGA CAAAGACTCA GACACCGAAA GTTTAGTTCA ATCGAAATCC
    CCCAGCAGCC CCAGTCCCTC CTCCTGTCTT TCATCTACAG AGCATCACCT GGCAGTGAAC
    GCGCTCAGAA ACTTGGGACT GCTAAAACAG TTACTGGTGC AGCAGCTGAA GCTCCTTCAG
    AAAGAGGAAG CCGAGAACTT TCAACGATTT TGCAGCCGTA AAACAAAGGA GCAGATGAGG
    GAGAGTGAAA AACTTACTAA GTTTTCAAGC TTGGATAAGC AGAACTGGGG AAGCAGGGAG
    TCCGATTTGA ACAAAGAACT GCATGCTCAG CAAAGGGCCA CAGACAGACG TTCTGAACAT
    AGCAACACTG GCCCAGACTG TGTTGCTGAG GTATTGGAAG AGGGTGGGGA ACAGTATTTT
    GATGCTCAGG AACCACAGAT TGATATGAAT GTAGATGGCT CTAAGAGAGA GAAGAACATT
    GAAGAGGCAC AGTTCAGATT ATCAGAAAAT TACCTGATTC TGGATGGCTA TGAGAACGTG
    CAGGAGAGCT CCACAGATGA TGATCTCTCA GCCTCACAGT ACCCCACCCA GTGCCCTGCT
    GTTAGTGATC CAGCCGATCC TTTGCATGTG GAAGAGAGAC TGACTTTCAG TGCCCTCGAT
    CCTACACCAT CTTCTGAGGC TTTTAACCAC ATGCTTCAGT ACATCGCAAA CATTGAATCA
    GACTTGCAGC AGCTTAGGGG GCTTGAAGAG TTTTATAACA CATATCGAAA CAGAGTGGCT
    ACTTCAGGGG TCTTGGAGGA AGATCCAGAT AAGGCAATGC TTCCTACAGA TAAGGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61526
    Clone ID Related Accession (Same CDS sequence) XM_031905189.1
    Accession Version XM_031905189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA
    CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC
    CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG
    GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT
    GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC
    TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT
    CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC
    CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA
    GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA
    AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA
    AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA
    CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT
    ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG
    TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC
    TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT
    CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA
    AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA
    GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA
    TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT
    GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC
    ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA
    TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG
    CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGGTTTAC TGACCTGGGA
    ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT
    GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG
    GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG
    TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG
    CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA
    GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT
    TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG
    GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG
    GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA
    GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG
    AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT
    GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT
    CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC
    CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG
    ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA
    ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC
    AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC
    TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG
    GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC
    TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG
    ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA
    GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC
    TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC
    AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG
    CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA
    GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG
    AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC
    GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC
    AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT
    GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA
    GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG
    GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT
    AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT
    ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC
    TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT
    TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761049.1
    CDS779..5035
    Translation

    Target ORF information:

    RefSeq Version XM_031905189.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCGGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    CAGGGTTCTG AATCACTGCA GATCAGTGAG CTTGATGCAG ACTCTAGCGA CATACTGGGA
    CGCACAGATT ACAGCAGTGG GGATGGATCA CGACCAAACA GTGACATTGC AGATAGCCCC
    CGTAGTGGAC AGCGAGAGAA AAGCTTCCTG GAGGAAAGTC CTGAGAAAAG TGAGATTCAG
    GACACGGACT CACAGTCAAG TATGGGAAGC CCCTCTATTC GCTTCACTCA TAATATTATT
    GGTGCAGAGG ATGATGACTT TGATGCCGAA CCAGAGCAGC AGATGAATGG GCAGTGTAGC
    TGTTTCCAGA GCATGGACCT GTTAAAATCA CACCCAGCTC ACCTAGCAGT ATTTATACAT
    CATGTGGTCT CCCAATTTGA TCCAGCAGCC ATGCTGTGCT ACCTCTATAC AGACTTGTAC
    CAGCAAACAA ATTCTAAAGA GACTCGCAGG GTGTTTCTGG AGTTCAACCA ATTCTTCTTA
    GAAAAAGCCG GGAATCTTAG AGTTTCTGTC CCAGATGATG TTGCTCTTGA ATTAGAGAAA
    AGGAGACCAG AGCTGATTCC AGAGGAGCTA CATCGACAAT ACATCCAGAC TATGCAAGAA
    AGGCTCTCTG AGGATGTTAA AAAAAACTTG GAGGATTTTA AGCAAAAGCG CAGCATGGGA
    CTGACCTTGG CAGAGGGAGA GCTCTCCAAA CTGGATGCAG ATCGATTGAG AGACCGCAAT
    ACAATGGAGA AGGAAAGAGT CTGTGCAGAG CAGATTCTTC AGAAGATTGA TGAAATTCTG
    TTTTCATCAC AGACTCCTGA AGATGAAAAG AGCAATACCA TACAATATGT GATTCTAACC
    TATATGAAGC ATCTTGGTGT GAAAGTTAAA GAGCCACGAA GTCTTGAACA GAAACGTGGT
    CGAATTGGAT TCCTCCCAAA AATTAAGCAA AGTATGAAGA AAGATCGGGA GGGTGAGGAA
    AAGGTGAAGC GAAGAGGGTT TCCCAGCATC CTTGCACCTC AGAGGAAGCC TAGCCGACAA
    GATAGTAATG CAATTGGCAG GGCTATGGAA CTTCAGCGGC CACGCCACCC CAAGCACTTA
    TCCACATCTT CATCCAGTAG CCCTGAACCA GCAGATGGAA GTAGATTGCG CACTAGCGGT
    GGCTCCATTG AGGGTTCTGA CACACCGTTC TCTCCATCAT CTAATTTGGG TGCAGCATAC
    ACCTTTCAGG AAGTTGGCAG AGAAGTGGAA GGAAATTTTA GGTGTGGTGA AAATATGTCA
    TCAGCTGATA CCCTTGATGG TGGTACTCGC ACATCTGGCC CAATATTTGA CTTTCCATCG
    CCACCACTTG ACCACCTACA TGAAGAGACA GGTGAAGCAG ACAGGTTTAC TGACCTGGGA
    ACACCAAAAC CTTTCAGAAA GTTGGATAGC CTGGTTGTAG GTGACACTCA GAGTGAGGAT
    GAGCACCATG AGATTGACAA GGAACAAGAT CCACCCAACT GGCAGCAGCT TGTTAGTAAG
    GAAATACTGC AGAAACTTAA ACCGCACGAG ATCAAGCAAC AGGAAGTCAT ACACGAGCTG
    TTTTACACTG AAAGGGCACA TGTACAGAAA CTGGGTGTTT TGGACCAAGT GTTTCATCAG
    CGCGTGATCC GTGAGAATCT CTTAACACCT GCAGAGACCC GAAATGTTTT CTCCAACTTA
    GAAGAAATCT TACAGCTTCA TGTGGCACTT AATGAGCAAA TGAAAGCAGT AAAGAAGCGT
    TATGAAACAT CTGTGGTGGA ACAAATTGGA GATGATGTTC TGAACTGGTT CAGTGGAGCG
    GAAGAGGAGA AGCTAAAGCA GGCAGTGGCC ACATTTTGCA GTAACCAGCC CTTTGCGTTG
    GAGATGATAA AATCTCGCCA AAAGAAAGAT CTAAAATTCC TAACCTTTGT ACAGGATGCA
    GAGAGTAATC CTCTGTGTAA GCGCCTGCAG TTGAAGGATA TTATTCCCAC TGAGATGCAG
    AGACTAACCA AGTATCCACT GCTGCTGGAT AACATTGCCA GATATTCAGA TCGAGAAGAT
    GAGAAAGAAA AGGTGAACAG AGCAGCAAAC CACTGCCGTA ACATCTTGGA CTACGTAAAT
    CAGGCAGTTA AAGAGGCAGA AAACAAGCAG AGACTTGAAG ATTATCAACG CAGGCTGGAC
    CTGTCCTATT TGAAGCCAGG AGAGTATCCC ATGATAGACG AACTGAGAAA TCTAGATTTG
    ACCAAGAGAA AATTGATACA TGAGGGGCCT CTGACATGGA AGGTTAATAG AGACAAATCA
    ATCGATCTTT ACTCTCTGCT GCTAGAGGAC ATTTTGGTTT TACTTCAGAG ACAAGATGAC
    AAGTTGATTT TGAGGTGTCA CAGCAAGATT CTGGCTTCAA CTTCAGACAG CAAACATATC
    TTCAGCCCTG TTATCAAACT CAACACCGTG TTAGTACGGC AGGTGGCAAC AGATAACAAG
    GCATTCTTTG TTATATCCAT GTCAAAAAAT GGGGCACAGA TTTACGAGCT CATTGCCAAC
    TCTGTTTCCG AGAAAAATGG GTGGCAAGAC CTCATAACAA AAATGGCTGC AACATCGAAG
    ACTGAATATC CTCAACCTAC TCAGTTACAA CAGACTGCCC CAGAGATGGT CAGCGAAGAA
    GAACATGAAG ATGTGGAGCA ACAGAAAGTG TCACATGATC AACAAGAAGA TAGACTTATC
    TATAGGCACA GTACAGACAA AGACTCAGAC ACCGAAAGTT TAGTTCAATC GAAATCCCCC
    AGCAGCCCCA GTCCCTCCTC CTGTCTTTCA TCTACAGAGC ATCACCTGGC AGTGAACGCG
    CTCAGAAACT TGGGACTGCT AAAACAGTTA CTGGTGCAGC AGCTGAAGCT CCTTCAGAAA
    GAGGAAGCCG AGAACTTTCA ACGATTTTGC AGCCGTAAAA CAAAGGAGCA GATGAGGGAG
    AGTGAAAAAC TTACTAAGTT TTCAAGCTTG GATAAGCAGA ACTGGGGAAG CAGGGAGTCC
    GATTTGAACA AAGAACTGCA TGCTCAGCAA AGGGCCACAG ACAGACGTTC TGAACATAGC
    AACACTGGCC CAGACTGTGT TGCTGAGGTA TTGGAAGAGG GTGGGGAACA GTATTTTGAT
    GCTCAGGAAC CACAGATTGA TATGAATGTA GATGGCTCTA AGAGAGAGAA GAACATTGAA
    GAGGCACAGT TCAGATTATC AGAAAATTAC CTGATTCTGG ATGGCTATGA GAACGTGCAG
    GAGAGCTCCA CAGATGATGA TCTCTCAGCC TCACAGTACC CCACCCAGTG CCCTGCTGTT
    AGTGATCCAG CCGATCCTTT GCATGTGGAA GAGAGACTGA CTTTCAGTGC CCTCGATCCT
    ACACCATCTT CTGAGGCTTT TAACCACATG CTTCAGTACA TCGCAAACAT TGAATCAGAC
    TTGCAGCAGC TTAGGGGGCT TGAAGAGTTT TATAACACAT ATCGAAACAG AGTGGCTACT
    TCAGGGGTCT TGGAGGAAGA TCCAGATAAG GCAATGCTTC CTACAGATAA GGGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22766
    Clone ID Related Accession (Same CDS sequence) NM_001079028.1
    Accession Version NM_001079028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 12
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121927.1. ##Evidence-Data-START## Transcript exon combination :: BC121927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCTGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAA GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCGACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072496.1
    CDS109..4362
    Translation

    Target ORF information:

    RefSeq Version NM_001079028.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 12 (arhgef12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGGAA CACAGTCTGC AGCTTCCGAC AGGTTTCACT CCAGAAAACC AACCAGGCAC 
    GGAAGCATAC TTTATAGAGA GTCTTCTACA GACAAAAAGC AGAAAGTTGA AAGGAGTGTT
    AGCTCATCCC ATGACTTTGA TGCCACAGGT TTAGTTCAGC GCTGTGTGGT TATCCAACGT
    GATGAAAATG GCTTTGGCCT GACAGTCAGT GGCGATAACC CTGTCTTTGT TCAGTCTGTC
    AAAGAAGATG GCGCTGCCAT GCGAGCTGGA GTTCAGACTG GGGATCGGAT CATCAAGGTT
    AATGGCACTT TGGTCACACA CTCCAATCAT ATAGAAGTGG TCAAACTCAT AAAGTCTGGC
    TCCTATGTGG CTCTTACAGT ACAAGGTCGT CCACCTGGGT CACCCACCAT CCCTTTAACT
    GATTCTGAGG CAGATGTGCA CAATTTAGGT CCCATAATGC CTCCTCATTC TCCAGTAACC
    CAAGGCCATG GAAAAGGTAT TACAAGCCCT ATACTTATGG AGGAGGAAAA TAACATTGTG
    CATACTCAGA AACTGGACAT ATTGAAGAAG ATGCTCCAGA AAGAACAAGA CCAGCTACAA
    CAATTTCAAG AAGAGTATAA CTCTACTCCA TCACCAAGGC TTTCTAAGGA GATCCAAGAC
    AGCAAAAAAC ACATAGCCCA GTTGCAGGAG CGATTGTTGA AAGCCAGAGG TCTTTCACAG
    GGTTCTGAAT CACTGCAGAT CAGTGAGCTT GATGCAGACT CTAGCGACAT ACTGGGACGC
    ACAGATTACA GCAGTGGGGA TGGATCACGA CCAAACAGTG ACATTGCAGA TAGCCCCCGT
    AGTGGACAGC GAGAGAAAAG CTTCCTGGAA GAAAGTCCTG AGAAAAGTGA GATTCAGGAC
    ACGGACTCAC AGTCAAGTAT GGGAAGCCCC TCTATTCGCT TCACTCATAA TATTATTGGT
    GCAGAGGATG ATGACTTTGA TGCCGAACCA GAGCAGCAGA TGAATGGGCA GTGTAGCTGT
    TTCCAGAGCA TGGACCTGTT AAAATCACAC CCAGCTCACC TAGCAGTATT TATACATCAT
    GTGGTCTCCC AATTTGATCC AGCAGCCATG CTGTGCTACC TCTATACAGA CTTGTACCAG
    CAAACAAATT CTAAAGAGAC TCGCAGGGTG TTTCTGGAGT TCAACCAATT CTTCTTAGAA
    AAAGCCGGGA ATCTTAGAGT TTCTGTCCCA GATGATGTTG CTCTTGAATT AGAGAAAAGG
    AGACCAGAGC TGATTCCAGA GGAGCTACAT CGACAATACA TCCAGACTAT GCAAGAAAGG
    CTCTCTGAGG ATGTTAAAAA AAACTTGGAG GATTTTAAGC AAAAGCGCAG CATGGGACTG
    ACCTTGGCAG AGGGAGAGCT CTCCAAACTG GATGCAGATC GATTGAGAGA CCGCAATACA
    ATGGAGAAGG AAAGAGTCTG TGCAGAGCAG ATTCTTCAGA AGATTGATGA AATTCTGTTT
    TCATCACAGA CTCCTGAAGA TGAAAAGAGC AATACCATAC AATATGTGAT TCTAACCTAT
    ATGAAGCATC TTGGTGTGAA AGTTAAAGAG CCACGAAGTC TTGAACAGAA ACGTGGTCGA
    ATTGGATTCC TCCCAAAAAT TAAGCAAAGT ATGAAGAAAG ATCGGGAGGG TGAGGAAAAG
    GTGAAGCGAA GAGGGTTTCC CAGCATCCTT GCACCTCAGA GGAAGCCTAG CCGACAAGAT
    AGTAATGCAA TTGGCAGGGC TATGGAACTT CAGCGGCCAC GCCACCCCAA GCACTTATCC
    ACATCTTCAT CCAGTAGCCC TGAACCAGCA GATGGAAGTA GATTGCGCAC TAGCGGTGGC
    TCCATTGAGG GTTCTGACAC ACCGTTCTCT CCATCATCTA ATTTGGGTGC AGCATACACC
    TTTCAGGAAG TTGGCAGAGA AGTGGAAGGA AATTTTAGGT GTGGTGAAAA TATGTCATCA
    GCTGATACCC TTGATGGTGG TACTCGCACA TCTGGCCCAA TATTTGACTT TCCATCGCCA
    CCACTTGACC ACCTACATGA AGAGACAGGT GAAGCAGACA GGTTTACTGA CCTGGGAACA
    CCAAAACCTT TCAGAAAGTT GGATAGCCTG GTTGTAGGTG ACACTCAGAG TGAGGATGAG
    CACCATGAGA TTGACAAGGA ACAAGATCCA CCCAACTGGC AGCAGCTTGT TAGTAAGGAA
    ATACTGCAGA AACTTAAACC GCACGAGATC AAGCAACAGG AAGTCATACA CGAGCTGTTT
    TACACTGAAA GGGCACATGT ACAGAAACTG GGTGTTTTGG ACCAAGTGTT TCATCAGCGC
    GTGATCCGTG AGAATCTCTT AACACCTGCA GAGACCCGAA ATGTTTTCTC CAACTTAGAA
    GAAATCTTAC AGCTTCATGT GGCACTTAAT GAGCAAATGA AAGCAGTAAA GAAGCGTTAT
    GAAACATCTG TGGTGGAACA AATTGGAGAT GATGTTCTGA ACTGGTTCAG TGGAGCGGAA
    GAGGAGAAGC TAAAGCAGGC AGTGGCCACA TTTTGCAGTA ACCAGCCCTT TGCGTTGGAG
    ATGATAAAAT CTCGCCAAAA GAAAGATCTA AAATTCCTAA CCTTTGTACA GGATGCAGAG
    AGTAATCCTC TGTGTAAGCG CCTGCAGTTG AAGGATATTA TTCCCACTGA GATGCAGAGA
    CTAACCAAGT ATCCACTGCT GCTGGATAAC ATTGCCAGAT ATTCAGATCG AGAAGATGAG
    AAAGAAAAGG TGAACAGAGC AGCAAACCAC TGCCGTAACA TCTTGGACTA CGTAAATCAG
    GCAGTTAAAG AGGCAGAAAA CAAGCAGAGA CTTGAAGATT ATCAACGCAG GCTGGACCTG
    TCCTATTTGA AGCCAGGAGA GTATCCCATG ATAGACGAAC TGAGAAATCT AGATTTGACC
    AAGAGAAAAT TGATACATGA GGGGCCTCTG ACATGGAAGG TTAATAGAGA CAAATCAATC
    GATCTTTACT CTCTGCTGCT AGAGGACATT TTGGTTTTAC TTCAGAGACA AGATGACAAG
    TTGATTTTGA GGTGTCACAG CAAGATTCTG GCTTCGACTT CAGACAGCAA ACATATCTTC
    AGCCCTGTTA TCAAACTCAA CACCGTGTTA GTACGGCAGG TGGCAACAGA TAACAAGGCA
    TTCTTTGTTA TATCCATGTC AAAAAATGGG GCACAGATTT ACGAGCTCAT TGCCAACTCT
    GTTTCCGAGA AAAATGGGTG GCAAGACCTC ATAACAAAAA TGGCTGCAAC ATCGAAGACT
    GAATATCCTC AACCTACTCA GTTACAACAG ACTGCCCCAG AGATGGTCAG CGAAGAAGAA
    CATGAAGATG TGGAGCAACA GAAAGTGTCA CATGATCAAC AAGAAGATAG ACTTATCTAT
    AGGCACAGTA CAGACAAAGA CTCAGACACC GAAAGTTTAG TTCAATCGAA ATCCCCCAGC
    AGCCCCAGTC CCTCCTCCTG TCTTTCATCT ACAGAGCATC ACCTGGCAGT GAACGCGCTC
    AGAAACTTGG GACTGCTAAA ACAGTTACTG GTGCAGCAGC TGAAGCTCCT TCAGAAAGAG
    GAAGCCGAGA ACTTTCAACG ATTTTGCAGC CGTAAAACAA AGGAGCAGAT GAGGGAGAGT
    GAAAAACTTA CTAAGTTTTC AAGCTTGGAT AAGCAGAACT GGGGAAGCAG GGAGTCCGAT
    TTGAACAAAG AACTGCATGC TCAGCAAAGG GCCACAGACA GACGTTCTGA ACATAGCAAC
    ACTGGCCCAG ACTGTGTTGC TGAGGTATTG GAAGAGGGTG GGGAACAGTA TTTTGATGCT
    CAGGAACCAC AGATTGATAT GAATGTAGAT GGCTCTAAGA GAGAGAAGAA CATTGAAGAG
    GCACAGTTCA GATTATCAGA AAATTACCTG ATTCTGGATG GCTATGAGAA CGTGCAGGAG
    AGCTCCACAG ATGATGATCT CTCAGCCTCA CAGTACCCCA CCCAGTGCCC TGCTGTTAGT
    GATCCAGCCG ATCCTTTGCA TGTGGAAGAG AGACTGACTT TCAGTGCCCT CGATCCTACA
    CCATCTTCTG AGGCTTTTAA CCACATGCTT CAGTACATCG CAAACATTGA ATCAGACTTG
    CAGCAGCTTA GGGGGCTTGA AGAGTTTTAT AACACATATC GAAACAGAGT GGCTACTTCA
    GGGGTCTTGG AGGAAGATCC AGATAAGGCA ATGCTTCCTA CAGATAAGGG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.