flna cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following flna gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flna cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20791 NM_001078918.1
Latest version!
Xenopus tropicalis filamin A (flna), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa29245 NM_001374713.1
Latest version!
Xenopus tropicalis filamin A (flna), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29246 XM_012968611.3
Latest version!
Xenopus tropicalis filamin A (flna), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29247 XM_012968612.3
Latest version!
Xenopus tropicalis filamin A (flna), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa46055 XM_018096310.2
Latest version!
Xenopus tropicalis filamin A (flna), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa46056 XM_018096311.2
Latest version!
Xenopus tropicalis filamin A (flna), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa29245 XM_012968610.2
Latest version!
Xenopus tropicalis filamin A (flna), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OXa29247 XM_012968612.2 Xenopus tropicalis filamin A (flna), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OXa46055 XM_018096310.1 Xenopus tropicalis filamin A (flna), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OXa46056 XM_018096311.1 Xenopus tropicalis filamin A (flna), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OXa29246 XM_012968611.2 Xenopus tropicalis filamin A (flna), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa20791
    Clone ID Related Accession (Same CDS sequence) NM_001078918.1
    Accession Version NM_001078918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-27
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121570.1. ##Evidence-Data-START## Transcript exon combination :: BC121570.1, CX802641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTATCTG CATATGGCCC TGGGTTAGAA GGAGGCACCA CAGGCAGTGC TGCAGAGTTC 
    ATTGTCAACA CCACAAAAGC AGGCCCTGGG GCACTCACAG TCACAATTGA TGGCCCGTCC
    AAAGTCAAAA TGGACTGCCA GGAATGTGCC GAGGGCTATA AGGTCACCTA CACTCCTATG
    GCTCCTGGAA GCTACCTGAT ATCCATCAAG TACGGGGGAC CCTACCATAT TGTGGGAAGC
    CCATTCAAAG CAAAGGTTAC AGGTCCTCGC CTAGTGACCA GCCACAGCCT ACATGAATCC
    TCTTCAGTCT TTGTTGATTC TGTCACCAAA TCTGAGGCCT TCACTCAGCA TGCCGCAGCT
    CCTAGGATAG CATCAGACGC CAGCAAAGTA GTGGCCAAAG GGCTAGGACT CAACAAAGCC
    TTTGTAGGCC AGAAGAACTC TTTCACTGTG GACTGCAGTA AAGCTGGTAA CAACATGCTG
    CTTGTCGGAG TTCATGGACC GAAAACCCCA TGTGAAGAGA TAGTGGTGAA GCACCTTGGG
    AACCGTCTCT ACAACGTTAC GTACCTCCTG AAGGATAAGG GGGATTACAT CCTTGTAGTA
    AAATGGGGCG ATGAGCACAT TCTGGGAAGC CCTTACCATG TGTCTGTGCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072386.1
    CDS470..1123
    Translation

    Target ORF information:

    RefSeq Version NM_001078918.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001078918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTATCTG CATATGGCCC TGGGTTAGAA GGAGGCACCA CAGGCAGTGC TGCAGAGTTC 
    ATTGTCAACA CCACAAAAGC AGGCCCTGGG GCACTCACAG TCACAATTGA TGGCCCGTCC
    AAAGTCAAAA TGGACTGCCA GGAATGTGCC GAGGGCTATA AGGTCACCTA CACTCCTATG
    GCTCCTGGAA GCTACCTGAT ATCCATCAAG TACGGGGGAC CCTACCATAT TGTGGGAAGC
    CCATTCAAAG CAAAGGTTAC AGGTCCTCGC CTAGTGACCA GCCACAGCCT ACATGAATCC
    TCTTCAGTCT TTGTTGATTC TGTCACCAAA TCTGAGGCCT TCACTCAGCA TGCCGCAGCT
    CCTAGGATAG CATCAGACGC CAGCAAAGTA GTGGCCAAAG GGCTAGGACT CAACAAAGCC
    TTTGTAGGCC AGAAGAACTC TTTCACTGTG GACTGCAGTA AAGCTGGTAA CAACATGCTG
    CTTGTCGGAG TTCATGGACC GAAAACCCCA TGTGAAGAGA TAGTGGTGAA GCACCTTGGG
    AACCGTCTCT ACAACGTTAC GTACCTCCTG AAGGATAAGG GGGATTACAT CCTTGTAGTA
    AAATGGGGCG ATGAGCACAT TCTGGGAAGC CCTTACCATG TGTCTGTGCC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29245
    Clone ID Related Accession (Same CDS sequence) XM_012968610.2 , NM_001374713.1
    Accession Version NM_001374713.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-08
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03030610.1. On Oct 9, 2019 this sequence version replaced XM_012968610.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTCTG
    GACGGCGAGC AAATGATGCC TCAGCAGATG GTTCAACAGC AGTTAGCCCC ACCATACGCT
    TACCCGGGCA TGCAACAACC CTGGGCTACT GATCGTCCAG TTATGGGTGT AAATGGACTG
    GATGTGGCAG GACTTAGACC TTTTGATCTG GTTATTCCCT TCACCATTAA AAAAGGAGAG
    ATTACAGGAG AAGTGCGAAT GCCATCAGGG AAGGTGGCCA AGCCTGACAT CACTGATAAC
    AAAGATGGAA CAGTCACGGT GAGATATGCT CCCACTGAAG CTGGACTCCA TGAGATGGAT
    ATAAAGTATG ATGGCATGCA TATTCCAGGA AGCCCCCTAC AGTTTTATGT GGATTATGTA
    AACAGTGGCC ATGTTACTGC CTATGGCCCA GGCTTGATTC ATGGAACAGT GAATAAACCT
    GCTGTCTTCA CTGTGAACAC TAAGGATGCT GGAGAAGGTG GCTTGTCACT AGCTATTGAG
    GGCCCCTCCA AAGCAGAGAT CAGCTGCACA GATAACCAGG ATGGGACCTG CACAGTTTCT
    TATCTCCCTG TGCTCCCTGG AGACTACAAC ATCCTAGTCA AGTATAACAA CAAGCATATT
    CCGGGAAGTC CATTTGTTGC TAAAATAACA GGAGATGATT CTATGCGTAT GTCCCAGCTG
    AAGGTTGGGT CTGCAGCAGA CATCCCCCTG AACATTGTAG AGACAGACCT GAGCCAGCTG
    ACTGCTACAG TGACCTCTCC ATCTGGAAGA GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA
    AACGGCCATG TTGGCATCTC GTTTGTGCCA AAGGAGACAG GCGAACACAT TGTTAGCATA
    AAAAAAAATG GTATCCACAT CCCCAACAGC CCAATCACTG TAATGATCAG CCAATCCGAA
    ATCGGGGATG CCAGCCGAGT GATAGTGTCT GGTCAAGGTC TGACTGAAGG GAGGACATTT
    GAGCCCGCAG AATTCATCAT TGACACCAGG GAAGCAGGTT ATGGTGGACT TAGCCTTTCC
    ATCGAGGGGC CCAGCAAAGT GGATATCAAC ACAGAAGACT TGGAAGATGG GACATGCAAA
    GTTACTTACT GCCCAACTGA ACCTGGAAAT TATATTATCA ACATAAAGTT TGCTGATCAG
    CATGTTCCTG GCAGCCCCTT CTCTGTCAAG GTGACTGGGG AAGGAAGAAT GAAGGAGAGT
    ATCACTCGAA GACGCACCGC TCCATCTGTG GCAAACATTG GTAGCCAGTG TGATCTGAGC
    CTAAAAATAC CCGAGATCAG CATTCATGAC ATGACTGCCC AGGTCACTAG TCCCTCAGGG
    AAGATTCATG ATGCAGAAAT AATGGAAAGC GAGAATAACA CTTACTGCAT CCGATTTGTT
    CCTTCTGAGA TGGGCGTCCA CACAGTCAGT GTTAAGTACA AGGGACAGCA TGTGCCTGGA
    AGCCCCTTTC AGTTCACTGT TGGCCCCTTG GGTGAAGGAG GAGCCCACAA AGTACGAGCT
    GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT GGAACACCAG CGGAGTTCAG TATCTGGACC
    AGAGAGGCAG GAGCTGGTGG TCTATCAATT GCAGTGGAAG GTCCAAGCAA AGCAGAGATT
    GCATTTGAGG ACAGAAAGGA TGGTTCTTGT GGAGTGTCCT ACATTGTGCA GGAGCCTGGT
    GATTATGAGG TGTCTGTGAA GTTTAATGAT GAGCACATAC CAGACAGCCC CTTCGTGGTT
    CCAGTGGGTT CGACATCAGA TGATGCACGG AGACTTACTG TTTCTAGTCT TCAGGAGTCA
    GGGTTAAAGG TCAACCAGCC AGCCTCTTTT GCAGTAAGTC TAAATGGTGC CAAAGGAGTT
    CTAGATGCCA AGGTCCACAG CCCCTCAGGT GCCTTGGAGG AATGCTGTGT GACAGAAATT
    GATGAAGATA AATATGCTGT GCGCTTTATT CCCCGTGAAA ATGGTCTATA TATTGTCGAT
    GTAAAGTTTA ACGGCTCCCA TATACCTGGC AGTCCCTTCA AAATAAGAGT AGGGGAGCCA
    GGCCAAGCTG GGGATCCAGG AATGGTATCT GCATATGGCC CTGGGTTAGA AGGAGGCACC
    ACAGGCAGTG CTGCAGAGTT CATTGTCAAC ACCACAAAAG CAGGCCCTGG GGCACTCACA
    GTCACAATTG ATGGCCCGTC CAAAGTCAAA ATGGACTGCC AGGAATGTGC CGAGGGCTAT
    AAGGTCACCT ACACTCCTAT GGCTCCTGGA AGCTACCTGA TATCCATCAA GTACGGGGGA
    CCCTACCATA TTGTGGGAAG CCCATTCAAA GCAAAGGTTA CAGGTCCTCG CCTAGTGACC
    AGCCACAGCC TACATGAATC CTCTTCAGTC TTTGTTGATT CTGTCACCAA ATCTGAGGCC
    TTCACTCAGC ATGCCGCAGC TCCTAGGATA GCATCAGACG CCAGCAAAGT AGTGGCCAAA
    GGGCTAGGAC TCAACAAAGC CTTTGTAGGC CAGAAGAACT CTTTCACTGT GGACTGCAGT
    AAAGCTGGTA ACAACATGCT GCTTGTCGGA GTTCATGGAC CGAAAACCCC ATGTGAAGAG
    ATAGTGGTGA AGCACCTTGG GAACCGTCTC TACAACGTTA CGTACCTCCT GAAGGATAAG
    GGGGATTACA TCCTTGTAGT AAAATGGGGC GATGAGCACA TTCTGGGAAG CCCTTACCAT
    GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361642.1
    CDS182..8116
    Translation

    Target ORF information:

    RefSeq Version NM_001374713.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374713.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTCTG
    GACGGCGAGC AAATGATGCC TCAGCAGATG GTTCAACAGC AGTTAGCCCC ACCATACGCT
    TACCCGGGCA TGCAACAACC CTGGGCTACT GATCGTCCAG TTATGGGTGT AAATGGACTG
    GATGTGGCAG GACTTAGACC TTTTGATCTG GTTATTCCCT TCACCATTAA AAAAGGAGAG
    ATTACAGGAG AAGTGCGAAT GCCATCAGGG AAGGTGGCCA AGCCTGACAT CACTGATAAC
    AAAGATGGAA CAGTCACGGT GAGATATGCT CCCACTGAAG CTGGACTCCA TGAGATGGAT
    ATAAAGTATG ATGGCATGCA TATTCCAGGA AGCCCCCTAC AGTTTTATGT GGATTATGTA
    AACAGTGGCC ATGTTACTGC CTATGGCCCA GGCTTGATTC ATGGAACAGT GAATAAACCT
    GCTGTCTTCA CTGTGAACAC TAAGGATGCT GGAGAAGGTG GCTTGTCACT AGCTATTGAG
    GGCCCCTCCA AAGCAGAGAT CAGCTGCACA GATAACCAGG ATGGGACCTG CACAGTTTCT
    TATCTCCCTG TGCTCCCTGG AGACTACAAC ATCCTAGTCA AGTATAACAA CAAGCATATT
    CCGGGAAGTC CATTTGTTGC TAAAATAACA GGAGATGATT CTATGCGTAT GTCCCAGCTG
    AAGGTTGGGT CTGCAGCAGA CATCCCCCTG AACATTGTAG AGACAGACCT GAGCCAGCTG
    ACTGCTACAG TGACCTCTCC ATCTGGAAGA GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA
    AACGGCCATG TTGGCATCTC GTTTGTGCCA AAGGAGACAG GCGAACACAT TGTTAGCATA
    AAAAAAAATG GTATCCACAT CCCCAACAGC CCAATCACTG TAATGATCAG CCAATCCGAA
    ATCGGGGATG CCAGCCGAGT GATAGTGTCT GGTCAAGGTC TGACTGAAGG GAGGACATTT
    GAGCCCGCAG AATTCATCAT TGACACCAGG GAAGCAGGTT ATGGTGGACT TAGCCTTTCC
    ATCGAGGGGC CCAGCAAAGT GGATATCAAC ACAGAAGACT TGGAAGATGG GACATGCAAA
    GTTACTTACT GCCCAACTGA ACCTGGAAAT TATATTATCA ACATAAAGTT TGCTGATCAG
    CATGTTCCTG GCAGCCCCTT CTCTGTCAAG GTGACTGGGG AAGGAAGAAT GAAGGAGAGT
    ATCACTCGAA GACGCACCGC TCCATCTGTG GCAAACATTG GTAGCCAGTG TGATCTGAGC
    CTAAAAATAC CCGAGATCAG CATTCATGAC ATGACTGCCC AGGTCACTAG TCCCTCAGGG
    AAGATTCATG ATGCAGAAAT AATGGAAAGC GAGAATAACA CTTACTGCAT CCGATTTGTT
    CCTTCTGAGA TGGGCGTCCA CACAGTCAGT GTTAAGTACA AGGGACAGCA TGTGCCTGGA
    AGCCCCTTTC AGTTCACTGT TGGCCCCTTG GGTGAAGGAG GAGCCCACAA AGTACGAGCT
    GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT GGAACACCAG CGGAGTTCAG TATCTGGACC
    AGAGAGGCAG GAGCTGGTGG TCTATCAATT GCAGTGGAAG GTCCAAGCAA AGCAGAGATT
    GCATTTGAGG ACAGAAAGGA TGGTTCTTGT GGAGTGTCCT ACATTGTGCA GGAGCCTGGT
    GATTATGAGG TGTCTGTGAA GTTTAATGAT GAGCACATAC CAGACAGCCC CTTCGTGGTT
    CCAGTGGGTT CGACATCAGA TGATGCACGG AGACTTACTG TTTCTAGTCT TCAGGAGTCA
    GGGTTAAAGG TCAACCAGCC AGCCTCTTTT GCAGTAAGTC TAAATGGTGC CAAAGGAGTT
    CTAGATGCCA AGGTCCACAG CCCCTCAGGT GCCTTGGAGG AATGCTGTGT GACAGAAATT
    GATGAAGATA AATATGCTGT GCGCTTTATT CCCCGTGAAA ATGGTCTATA TATTGTCGAT
    GTAAAGTTTA ACGGCTCCCA TATACCTGGC AGTCCCTTCA AAATAAGAGT AGGGGAGCCA
    GGCCAAGCTG GGGATCCAGG AATGGTATCT GCATATGGCC CTGGGTTAGA AGGAGGCACC
    ACAGGCAGTG CTGCAGAGTT CATTGTCAAC ACCACAAAAG CAGGCCCTGG GGCACTCACA
    GTCACAATTG ATGGCCCGTC CAAAGTCAAA ATGGACTGCC AGGAATGTGC CGAGGGCTAT
    AAGGTCACCT ACACTCCTAT GGCTCCTGGA AGCTACCTGA TATCCATCAA GTACGGGGGA
    CCCTACCATA TTGTGGGAAG CCCATTCAAA GCAAAGGTTA CAGGTCCTCG CCTAGTGACC
    AGCCACAGCC TACATGAATC CTCTTCAGTC TTTGTTGATT CTGTCACCAA ATCTGAGGCC
    TTCACTCAGC ATGCCGCAGC TCCTAGGATA GCATCAGACG CCAGCAAAGT AGTGGCCAAA
    GGGCTAGGAC TCAACAAAGC CTTTGTAGGC CAGAAGAACT CTTTCACTGT GGACTGCAGT
    AAAGCTGGTA ACAACATGCT GCTTGTCGGA GTTCATGGAC CGAAAACCCC ATGTGAAGAG
    ATAGTGGTGA AGCACCTTGG GAACCGTCTC TACAACGTTA CGTACCTCCT GAAGGATAAG
    GGGGATTACA TCCTTGTAGT AAAATGGGGC GATGAGCACA TTCTGGGAAG CCCTTACCAT
    GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29246
    Clone ID Related Accession (Same CDS sequence) XM_012968611.2 , XM_012968611.3
    Accession Version XM_012968611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824065.1
    CDS176..8086
    Translation

    Target ORF information:

    RefSeq Version XM_012968611.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29247
    Clone ID Related Accession (Same CDS sequence) XM_012968612.2 , XM_012968612.3
    Accession Version XM_012968612.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968612.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACAGAG GTCTTTACGC TTGCAATCCC GCCGCCTGCC GTGCTGTGGG CCGTGGCTTG 
    CAGCCAAAGG GTGTACGCGT GAAGGAGACG GCGGATTTTA AGGTTTATAC TAAAGGCGCA
    GGAAGCGGTG AACTTAAAGT TGTGGTCAAG GGACCAAAGG GTACAGAGGA ACGTGTCAAG
    CAGAAGGACC TAGGAGATGG TGTGTATGCC TTTGAGTACT ATCCAGCTAC ACCTGGGAAC
    TATATTGTTA CCATCACATG GGGCGGACAG AATATCCCCA GAAGTCCATT TGAGGTAAAA
    GTTGGCACTG AATGTGGACA ACAAAGAGTT CGAGCATGGG GCCCTGGTCT TGAAGGAGGG
    GTGGTTGGAA AATCAGCTGA CTTTGTTGTA GAAGCAATCG GTGATGATGT TGGGACTCTC
    GGGTTTTCTG TGGAAGGTCC ATCTCAAGCT AAGATCGAGT GTGACGATAA AGGAGATGGG
    TCCTGTGATG TGCGCTACTG GCCACTGGAA CCTGGAGAGT ATGCGGTGCA TGTTCTGTGC
    AACAATGAGG ACATCAAACT GAGTCCTTTC ATGGCTGATA TCAAACCAGC CCCCAAAGAC
    TTCTACCCAG AGAAGGTCAA GGCCTATGGT CCTGGGCTAG AGAAGACTGG GTTGGCAATA
    AACAAACCAG CAGAGTTCAC TGTTGATGCC AAACTTGGAG GAAAGGCTCC TCTCAAGGTG
    TCTGTGCAGG ATTCTGAAGG CACTCCAGTG GATGTCACAA CCAAAGATAA TGGAAATGGC
    ACATACAGCT GCAGTTATTT GCCAAAGAAA CCTCTGAAAC ACACAGCTGT GGTGTCTTGG
    GGTGGGGTGA ACATTCCCAA CAGTCCTTTC AGGATGACTA TTGGTGCTGG AAGCCACCCT
    AACAAGGTGA AGGTTTATGG ACCTGGTGTT GCTAAGACTG GCCTGAAAGC TCATGAACCA
    ACCTACTTTA CTGTGGATTG CACAGAAGCT GGTCAAGGTG ATGTGAGTAT TGGAATTAAA
    TGTGCCCCAG GAGTTGTGGG CCCGGCTGAG GCGGACATAG ACTTTGACAT TATCCGCAAT
    GATAATGATA CATTCACCGT GAAGTACACT CCTCCTGGAG CTGGGAGCTA CACCATCATG
    GTCCTGTTTG CTGACCAGGC TACTCCAATG AGTCCATTCC GTATCAAGGT TGATCCATCA
    CATGATGCAA GTAAAGTTAA GGCAGAAGGA CCTGGACTAA ATAGAAGTGG TGTAGAAATT
    GGAAAACCAA CTCATTTCAC AGTTAACACC AAGGCTGCTG GAAAAGCCAA CCTAGACGTG
    CAGTTTACAG GTCCAGCCAA GGGAGAGGCT GTGAAGGACT TTGATGTTAT TGACAACCAC
    GATAACACCT ACACCGTAAA ATACACTCCA GTGCAACAGG GTAACATGGG TGTAAATGTG
    ACCTATGGTG GTGACCATAT TCCAAAGAGT CCCTTCACCG TTGGCATTGC CCCAACTTTG
    GACCTCAGTA AAATCAAAGT TTCTGGACTT GGTGACAAGG TGGAGGTCGG GAAGGATCAG
    GAGTTCACAG TAAAATCCAG GGGTGCTGGA GGACAAGGCA AAGTGGCTTG CAAGATCACA
    GGACCATCCA ACAAGTCTGT TCCTTGCAAG GTGGAACCTG GTCTGAGCCC AGATAACAGC
    ACTGTCAAAT TCATCCCTAG GGAGGAAGGC CCATATGAGG TAGACGTGAC GTACGACGGA
    GTGCCTATAC CTGGTAGCCC ATTTCCCGTT GAGGCAGTTC CTTCAACAAA TCCTTCAAAG
    GTCAAAGCTT TTGGACCTGG TCTAAAAGGA GGCAGTGTTG GTTCCTCTGC ACCCTTTACT
    ATTGACACTA AAGGTGCAGG CCAAGGTGGG CTTGGTCTCA CTGTTGAAGG TCCATGTGAA
    GCCAAAATAG AATGTCTAGA CAATGGAGAT GGAACCTGTT CTGTCTCTTA CTTGCCTACA
    GAACGTGGTG ACTATAATAT CAATATCTTG TTTGCTGATA CACATATCCC TGGATCACCT
    TTCAAGGCTA AGGTTGAGCC ATGTTTCGAT CCTTCAAAGG TGACCTGTTC TGGTCCTGGT
    CTGGAACATG CTCAGGTTGG AGAAGCTGGT AAATTTAATG TGGACTGTTC AAATGCTGGC
    AGCGCTGAGC TTACCATTGA AATTATCTCA GACAATGGTA TGCAAGCCGA AGTACACATC
    CAGGACAATG GGGATGGAAC TTACACCATT ACATACATCC CACTGTACCC AGGGGTCTAC
    ACTATCACCA TTAAGTATGG AGGACAGATG GTGCCCAACT TCCCAAGTAA GCTGCAAGTT
    AAACCTGCCA TTGACACCTC TGGGGTTAAA GTCTATGGAC CAGGAGTTGA AGGGAAAGGG
    GTTTTCAGAG AAGCTACTAC AGATTTCAAT GTGGATGCTC GGGCTCTAAC TAAATCTGGA
    GGGCCTCATC TCAAGACCGT GGTCTCCAAT CCTTCTGGCA ACTTTACTGA GGCATTTGTG
    CAAGACAACG GAGATGGCAC ATACAAAGTG GAATATACTC CATATGAAGA GGGAATCCAC
    TCTGTTGATG TAATCTACGA CGGAAGCCCA GCCCCAAAAA GTCCCTTTGT GGTCCCTGTG
    ACAGAAGGGT GTGATCCAAG CCGTGTCAGA GTCCATGGCC CTGGCATTGT GAGCGGTACC
    ACAAACCAAC CAAACAAATT CACTGTGGAG ACAAGAGGAG CTGGTACAGG AGGGCTTGGC
    TTGGCTGTTG AAGGTCCATC AGAAGCAAAG ATGTCCTGTA CCGATAACAA GGATGGCAGC
    TGTTCTGTGG AATACGTACC GTACGAACCT GGCACATACA ACCTTAATGT CACATATGGT
    GGACATCAAG TTCCAGGTAG CCCCTTTAAA GTTCCAGTTA GCGATGTGGT CGATAGCTCA
    AAAGTCAAAT GTACCGGCCC TGGTTTGAGC CCCGGTCTTG TAAGAGCAAA CGTCCCTCAG
    TCATTCAGTG TGGACACAAG CAAAGCTGGA GTTGCTCCTC TGCAAGTCAA AGTTCAAGGT
    CCCAAGGGTA TTGTTGAACC AGTGGAAATT GTGGATAATG GAAATGGCGT CCAGACTGTC
    AACTATGTGC CAACTAGAGA AGGACCTTAT AACATCTCTG TACTTTATGG TGATGATGAG
    ATACCTCACA GCCCATTTAA GGTTAGGGTT CTGCCAACTC ATGATGCAAG TAAGGTGAAG
    GCAAGCGGAC CAGGCTTAAA CACCACTGGA GTTCCTGCCA GCTTGCCTGT GGAGTTCACT
    ATTGATGCCA AGGATGCTGG AGAGGGCCTG CTTGCTGTTC AGATAACTGA TCCAGAGGGC
    AAGCCCAAGA AGGCTACAAT CCGTGATAAT CAGGATGGCA CCTATACGGT GTCCTATGTG
    CCTGACATGA CTGGTCGGTA CACTATCCTG ATAAAGTATG GAGGCGATGA GATCCCTTAT
    TCTCCTTATC GCATCCGTGC GCTTCCCACT GGGGACGCCA GCAAGTGCAC AGTGACAGTG
    TCAATCGGAG GTCACGGGCT AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824066.1
    CDS57..6587
    Translation

    Target ORF information:

    RefSeq Version XM_012968612.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968612.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACAGAG GTCTTTACGC TTGCAATCCC GCCGCCTGCC GTGCTGTGGG CCGTGGCTTG 
    CAGCCAAAGG GTGTACGCGT GAAGGAGACG GCGGATTTTA AGGTTTATAC TAAAGGCGCA
    GGAAGCGGTG AACTTAAAGT TGTGGTCAAG GGACCAAAGG GTACAGAGGA ACGTGTCAAG
    CAGAAGGACC TAGGAGATGG TGTGTATGCC TTTGAGTACT ATCCAGCTAC ACCTGGGAAC
    TATATTGTTA CCATCACATG GGGCGGACAG AATATCCCCA GAAGTCCATT TGAGGTAAAA
    GTTGGCACTG AATGTGGACA ACAAAGAGTT CGAGCATGGG GCCCTGGTCT TGAAGGAGGG
    GTGGTTGGAA AATCAGCTGA CTTTGTTGTA GAAGCAATCG GTGATGATGT TGGGACTCTC
    GGGTTTTCTG TGGAAGGTCC ATCTCAAGCT AAGATCGAGT GTGACGATAA AGGAGATGGG
    TCCTGTGATG TGCGCTACTG GCCACTGGAA CCTGGAGAGT ATGCGGTGCA TGTTCTGTGC
    AACAATGAGG ACATCAAACT GAGTCCTTTC ATGGCTGATA TCAAACCAGC CCCCAAAGAC
    TTCTACCCAG AGAAGGTCAA GGCCTATGGT CCTGGGCTAG AGAAGACTGG GTTGGCAATA
    AACAAACCAG CAGAGTTCAC TGTTGATGCC AAACTTGGAG GAAAGGCTCC TCTCAAGGTG
    TCTGTGCAGG ATTCTGAAGG CACTCCAGTG GATGTCACAA CCAAAGATAA TGGAAATGGC
    ACATACAGCT GCAGTTATTT GCCAAAGAAA CCTCTGAAAC ACACAGCTGT GGTGTCTTGG
    GGTGGGGTGA ACATTCCCAA CAGTCCTTTC AGGATGACTA TTGGTGCTGG AAGCCACCCT
    AACAAGGTGA AGGTTTATGG ACCTGGTGTT GCTAAGACTG GCCTGAAAGC TCATGAACCA
    ACCTACTTTA CTGTGGATTG CACAGAAGCT GGTCAAGGTG ATGTGAGTAT TGGAATTAAA
    TGTGCCCCAG GAGTTGTGGG CCCGGCTGAG GCGGACATAG ACTTTGACAT TATCCGCAAT
    GATAATGATA CATTCACCGT GAAGTACACT CCTCCTGGAG CTGGGAGCTA CACCATCATG
    GTCCTGTTTG CTGACCAGGC TACTCCAATG AGTCCATTCC GTATCAAGGT TGATCCATCA
    CATGATGCAA GTAAAGTTAA GGCAGAAGGA CCTGGACTAA ATAGAAGTGG TGTAGAAATT
    GGAAAACCAA CTCATTTCAC AGTTAACACC AAGGCTGCTG GAAAAGCCAA CCTAGACGTG
    CAGTTTACAG GTCCAGCCAA GGGAGAGGCT GTGAAGGACT TTGATGTTAT TGACAACCAC
    GATAACACCT ACACCGTAAA ATACACTCCA GTGCAACAGG GTAACATGGG TGTAAATGTG
    ACCTATGGTG GTGACCATAT TCCAAAGAGT CCCTTCACCG TTGGCATTGC CCCAACTTTG
    GACCTCAGTA AAATCAAAGT TTCTGGACTT GGTGACAAGG TGGAGGTCGG GAAGGATCAG
    GAGTTCACAG TAAAATCCAG GGGTGCTGGA GGACAAGGCA AAGTGGCTTG CAAGATCACA
    GGACCATCCA ACAAGTCTGT TCCTTGCAAG GTGGAACCTG GTCTGAGCCC AGATAACAGC
    ACTGTCAAAT TCATCCCTAG GGAGGAAGGC CCATATGAGG TAGACGTGAC GTACGACGGA
    GTGCCTATAC CTGGTAGCCC ATTTCCCGTT GAGGCAGTTC CTTCAACAAA TCCTTCAAAG
    GTCAAAGCTT TTGGACCTGG TCTAAAAGGA GGCAGTGTTG GTTCCTCTGC ACCCTTTACT
    ATTGACACTA AAGGTGCAGG CCAAGGTGGG CTTGGTCTCA CTGTTGAAGG TCCATGTGAA
    GCCAAAATAG AATGTCTAGA CAATGGAGAT GGAACCTGTT CTGTCTCTTA CTTGCCTACA
    GAACGTGGTG ACTATAATAT CAATATCTTG TTTGCTGATA CACATATCCC TGGATCACCT
    TTCAAGGCTA AGGTTGAGCC ATGTTTCGAT CCTTCAAAGG TGACCTGTTC TGGTCCTGGT
    CTGGAACATG CTCAGGTTGG AGAAGCTGGT AAATTTAATG TGGACTGTTC AAATGCTGGC
    AGCGCTGAGC TTACCATTGA AATTATCTCA GACAATGGTA TGCAAGCCGA AGTACACATC
    CAGGACAATG GGGATGGAAC TTACACCATT ACATACATCC CACTGTACCC AGGGGTCTAC
    ACTATCACCA TTAAGTATGG AGGACAGATG GTGCCCAACT TCCCAAGTAA GCTGCAAGTT
    AAACCTGCCA TTGACACCTC TGGGGTTAAA GTCTATGGAC CAGGAGTTGA AGGGAAAGGG
    GTTTTCAGAG AAGCTACTAC AGATTTCAAT GTGGATGCTC GGGCTCTAAC TAAATCTGGA
    GGGCCTCATC TCAAGACCGT GGTCTCCAAT CCTTCTGGCA ACTTTACTGA GGCATTTGTG
    CAAGACAACG GAGATGGCAC ATACAAAGTG GAATATACTC CATATGAAGA GGGAATCCAC
    TCTGTTGATG TAATCTACGA CGGAAGCCCA GCCCCAAAAA GTCCCTTTGT GGTCCCTGTG
    ACAGAAGGGT GTGATCCAAG CCGTGTCAGA GTCCATGGCC CTGGCATTGT GAGCGGTACC
    ACAAACCAAC CAAACAAATT CACTGTGGAG ACAAGAGGAG CTGGTACAGG AGGGCTTGGC
    TTGGCTGTTG AAGGTCCATC AGAAGCAAAG ATGTCCTGTA CCGATAACAA GGATGGCAGC
    TGTTCTGTGG AATACGTACC GTACGAACCT GGCACATACA ACCTTAATGT CACATATGGT
    GGACATCAAG TTCCAGGTAG CCCCTTTAAA GTTCCAGTTA GCGATGTGGT CGATAGCTCA
    AAAGTCAAAT GTACCGGCCC TGGTTTGAGC CCCGGTCTTG TAAGAGCAAA CGTCCCTCAG
    TCATTCAGTG TGGACACAAG CAAAGCTGGA GTTGCTCCTC TGCAAGTCAA AGTTCAAGGT
    CCCAAGGGTA TTGTTGAACC AGTGGAAATT GTGGATAATG GAAATGGCGT CCAGACTGTC
    AACTATGTGC CAACTAGAGA AGGACCTTAT AACATCTCTG TACTTTATGG TGATGATGAG
    ATACCTCACA GCCCATTTAA GGTTAGGGTT CTGCCAACTC ATGATGCAAG TAAGGTGAAG
    GCAAGCGGAC CAGGCTTAAA CACCACTGGA GTTCCTGCCA GCTTGCCTGT GGAGTTCACT
    ATTGATGCCA AGGATGCTGG AGAGGGCCTG CTTGCTGTTC AGATAACTGA TCCAGAGGGC
    AAGCCCAAGA AGGCTACAAT CCGTGATAAT CAGGATGGCA CCTATACGGT GTCCTATGTG
    CCTGACATGA CTGGTCGGTA CACTATCCTG ATAAAGTATG GAGGCGATGA GATCCCTTAT
    TCTCCTTATC GCATCCGTGC GCTTCCCACT GGGGACGCCA GCAAGTGCAC AGTGACAGTG
    TCAATCGGAG GTCACGGGCT AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46055
    Clone ID Related Accession (Same CDS sequence) XM_018096310.1 , XM_018096310.2
    Accession Version XM_018096310.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTACT
    GATCGTCCAG TTATGGGTGT AAATGGACTG GATGTGGCAG GACTTAGACC TTTTGATCTG
    GTTATTCCCT TCACCATTAA AAAAGGAGAG ATTACAGGAG AAGTGCGAAT GCCATCAGGG
    AAGGTGGCCA AGCCTGACAT CACTGATAAC AAAGATGGAA CAGTCACGGT GAGATATGCT
    CCCACTGAAG CTGGACTCCA TGAGATGGAT ATAAAGTATG ATGGCATGCA TATTCCAGGA
    AGCCCCCTAC AGTTTTATGT GGATTATGTA AACAGTGGCC ATGTTACTGC CTATGGCCCA
    GGCTTGATTC ATGGAACAGT GAATAAACCT GCTGTCTTCA CTGTGAACAC TAAGGATGCT
    GGAGAAGGTG GCTTGTCACT AGCTATTGAG GGCCCCTCCA AAGCAGAGAT CAGCTGCACA
    GATAACCAGG ATGGGACCTG CACAGTTTCT TATCTCCCTG TGCTCCCTGG AGACTACAAC
    ATCCTAGTCA AGTATAACAA CAAGCATATT CCGGGAAGTC CATTTGTTGC TAAAATAACA
    GGAGATGATT CTATGCGTAT GTCCCAGCTG AAGGTTGGGT CTGCAGCAGA CATCCCCCTG
    AACATTGTAG AGACAGACCT GAGCCAGCTG ACTGCTACAG TGACCTCTCC ATCTGGAAGA
    GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA AACGGCCATG TTGGCATCTC GTTTGTGCCA
    AAGGAGACAG GCGAACACAT TGTTAGCATA AAAAAAAATG GTATCCACAT CCCCAACAGC
    CCAATCACTG TAATGATCAG CCAATCCGAA ATCGGGGATG CCAGCCGAGT GATAGTGTCT
    GGTCAAGGTC TGACTGAAGG GAGGACATTT GAGCCCGCAG AATTCATCAT TGACACCAGG
    GAAGCAGGTT ATGGTGGACT TAGCCTTTCC ATCGAGGGGC CCAGCAAAGT GGATATCAAC
    ACAGAAGACT TGGAAGATGG GACATGCAAA GTTACTTACT GCCCAACTGA ACCTGGAAAT
    TATATTATCA ACATAAAGTT TGCTGATCAG CATGTTCCTG GCAGCCCCTT CTCTGTCAAG
    GTGACTGGGG AAGGAAGAAT GAAGGAGAGT ATCACTCGAA GACGCACCGC TCCATCTGTG
    GCAAACATTG GTAGCCAGTG TGATCTGAGC CTAAAAATAC CCGAGATCAG CATTCATGAC
    ATGACTGCCC AGGTCACTAG TCCCTCAGGG AAGATTCATG ATGCAGAAAT AATGGAAAGC
    GAGAATAACA CTTACTGCAT CCGATTTGTT CCTTCTGAGA TGGGCGTCCA CACAGTCAGT
    GTTAAGTACA AGGGACAGCA TGTGCCTGGA AGCCCCTTTC AGTTCACTGT TGGCCCCTTG
    GGTGAAGGAG GAGCCCACAA AGTACGAGCT GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT
    GGAACACCAG CGGAGTTCAG TATCTGGACC AGAGAGGCAG GAGCTGGTGG TCTATCAATT
    GCAGTGGAAG GTCCAAGCAA AGCAGAGATT GCATTTGAGG ACAGAAAGGA TGGTTCTTGT
    GGAGTGTCCT ACATTGTGCA GGAGCCTGGT GATTATGAGG TGTCTGTGAA GTTTAATGAT
    GAGCACATAC CAGACAGCCC CTTCGTGGTT CCAGTGGGTT CGACATCAGA TGATGCACGG
    AGACTTACTG TTTCTAGTCT TCAGGAGTCA GGGTTAAAGG TCAACCAGCC AGCCTCTTTT
    GCAGTAAGTC TAAATGGTGC CAAAGGAGTT CTAGATGCCA AGGTCCACAG CCCCTCAGGT
    GCCTTGGAGG AATGCTGTGT GACAGAAATT GATGAAGATA AATATGCTGT GCGCTTTATT
    CCCCGTGAAA ATGGTCTATA TATTGTCGAT GTAAAGTTTA ACGGCTCCCA TATACCTGGC
    AGTCCCTTCA AAATAAGAGT AGGGGAGCCA GGCCAAGCTG GGGATCCAGG AATGGTATCT
    GCATATGGCC CTGGGTTAGA AGGAGGCACC ACAGGCAGTG CTGCAGAGTT CATTGTCAAC
    ACCACAAAAG CAGGCCCTGG GGCACTCACA GTCACAATTG ATGGCCCGTC CAAAGTCAAA
    ATGGACTGCC AGGAATGTGC CGAGGGCTAT AAGGTCACCT ACACTCCTAT GGCTCCTGGA
    AGCTACCTGA TATCCATCAA GTACGGGGGA CCCTACCATA TTGTGGGAAG CCCATTCAAA
    GCAAAGGTTA CAGGTCCTCG CCTAGTGACC AGCCACAGCC TACATGAATC CTCTTCAGTC
    TTTGTTGATT CTGTCACCAA ATCTGAGGCC TTCACTCAGC ATGCCGCAGC TCCTAGGATA
    GCATCAGACG CCAGCAAAGT AGTGGCCAAA GGGCTAGGAC TCAACAAAGC CTTTGTAGGC
    CAGAAGAACT CTTTCACTGT GGACTGCAGT AAAGCTGGTA ACAACATGCT GCTTGTCGGA
    GTTCATGGAC CGAAAACCCC ATGTGAAGAG ATAGTGGTGA AGCACCTTGG GAACCGTCTC
    TACAACGTTA CGTACCTCCT GAAGGATAAG GGGGATTACA TCCTTGTAGT AAAATGGGGC
    GATGAGCACA TTCTGGGAAG CCCTTACCAT GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951799.1
    CDS183..8027
    Translation

    Target ORF information:

    RefSeq Version XM_018096310.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTACT
    GATCGTCCAG TTATGGGTGT AAATGGACTG GATGTGGCAG GACTTAGACC TTTTGATCTG
    GTTATTCCCT TCACCATTAA AAAAGGAGAG ATTACAGGAG AAGTGCGAAT GCCATCAGGG
    AAGGTGGCCA AGCCTGACAT CACTGATAAC AAAGATGGAA CAGTCACGGT GAGATATGCT
    CCCACTGAAG CTGGACTCCA TGAGATGGAT ATAAAGTATG ATGGCATGCA TATTCCAGGA
    AGCCCCCTAC AGTTTTATGT GGATTATGTA AACAGTGGCC ATGTTACTGC CTATGGCCCA
    GGCTTGATTC ATGGAACAGT GAATAAACCT GCTGTCTTCA CTGTGAACAC TAAGGATGCT
    GGAGAAGGTG GCTTGTCACT AGCTATTGAG GGCCCCTCCA AAGCAGAGAT CAGCTGCACA
    GATAACCAGG ATGGGACCTG CACAGTTTCT TATCTCCCTG TGCTCCCTGG AGACTACAAC
    ATCCTAGTCA AGTATAACAA CAAGCATATT CCGGGAAGTC CATTTGTTGC TAAAATAACA
    GGAGATGATT CTATGCGTAT GTCCCAGCTG AAGGTTGGGT CTGCAGCAGA CATCCCCCTG
    AACATTGTAG AGACAGACCT GAGCCAGCTG ACTGCTACAG TGACCTCTCC ATCTGGAAGA
    GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA AACGGCCATG TTGGCATCTC GTTTGTGCCA
    AAGGAGACAG GCGAACACAT TGTTAGCATA AAAAAAAATG GTATCCACAT CCCCAACAGC
    CCAATCACTG TAATGATCAG CCAATCCGAA ATCGGGGATG CCAGCCGAGT GATAGTGTCT
    GGTCAAGGTC TGACTGAAGG GAGGACATTT GAGCCCGCAG AATTCATCAT TGACACCAGG
    GAAGCAGGTT ATGGTGGACT TAGCCTTTCC ATCGAGGGGC CCAGCAAAGT GGATATCAAC
    ACAGAAGACT TGGAAGATGG GACATGCAAA GTTACTTACT GCCCAACTGA ACCTGGAAAT
    TATATTATCA ACATAAAGTT TGCTGATCAG CATGTTCCTG GCAGCCCCTT CTCTGTCAAG
    GTGACTGGGG AAGGAAGAAT GAAGGAGAGT ATCACTCGAA GACGCACCGC TCCATCTGTG
    GCAAACATTG GTAGCCAGTG TGATCTGAGC CTAAAAATAC CCGAGATCAG CATTCATGAC
    ATGACTGCCC AGGTCACTAG TCCCTCAGGG AAGATTCATG ATGCAGAAAT AATGGAAAGC
    GAGAATAACA CTTACTGCAT CCGATTTGTT CCTTCTGAGA TGGGCGTCCA CACAGTCAGT
    GTTAAGTACA AGGGACAGCA TGTGCCTGGA AGCCCCTTTC AGTTCACTGT TGGCCCCTTG
    GGTGAAGGAG GAGCCCACAA AGTACGAGCT GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT
    GGAACACCAG CGGAGTTCAG TATCTGGACC AGAGAGGCAG GAGCTGGTGG TCTATCAATT
    GCAGTGGAAG GTCCAAGCAA AGCAGAGATT GCATTTGAGG ACAGAAAGGA TGGTTCTTGT
    GGAGTGTCCT ACATTGTGCA GGAGCCTGGT GATTATGAGG TGTCTGTGAA GTTTAATGAT
    GAGCACATAC CAGACAGCCC CTTCGTGGTT CCAGTGGGTT CGACATCAGA TGATGCACGG
    AGACTTACTG TTTCTAGTCT TCAGGAGTCA GGGTTAAAGG TCAACCAGCC AGCCTCTTTT
    GCAGTAAGTC TAAATGGTGC CAAAGGAGTT CTAGATGCCA AGGTCCACAG CCCCTCAGGT
    GCCTTGGAGG AATGCTGTGT GACAGAAATT GATGAAGATA AATATGCTGT GCGCTTTATT
    CCCCGTGAAA ATGGTCTATA TATTGTCGAT GTAAAGTTTA ACGGCTCCCA TATACCTGGC
    AGTCCCTTCA AAATAAGAGT AGGGGAGCCA GGCCAAGCTG GGGATCCAGG AATGGTATCT
    GCATATGGCC CTGGGTTAGA AGGAGGCACC ACAGGCAGTG CTGCAGAGTT CATTGTCAAC
    ACCACAAAAG CAGGCCCTGG GGCACTCACA GTCACAATTG ATGGCCCGTC CAAAGTCAAA
    ATGGACTGCC AGGAATGTGC CGAGGGCTAT AAGGTCACCT ACACTCCTAT GGCTCCTGGA
    AGCTACCTGA TATCCATCAA GTACGGGGGA CCCTACCATA TTGTGGGAAG CCCATTCAAA
    GCAAAGGTTA CAGGTCCTCG CCTAGTGACC AGCCACAGCC TACATGAATC CTCTTCAGTC
    TTTGTTGATT CTGTCACCAA ATCTGAGGCC TTCACTCAGC ATGCCGCAGC TCCTAGGATA
    GCATCAGACG CCAGCAAAGT AGTGGCCAAA GGGCTAGGAC TCAACAAAGC CTTTGTAGGC
    CAGAAGAACT CTTTCACTGT GGACTGCAGT AAAGCTGGTA ACAACATGCT GCTTGTCGGA
    GTTCATGGAC CGAAAACCCC ATGTGAAGAG ATAGTGGTGA AGCACCTTGG GAACCGTCTC
    TACAACGTTA CGTACCTCCT GAAGGATAAG GGGGATTACA TCCTTGTAGT AAAATGGGGC
    GATGAGCACA TTCTGGGAAG CCCTTACCAT GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46055
    Clone ID Related Accession (Same CDS sequence) XM_018096310.1 , XM_018096310.2
    Accession Version XM_018096310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTACT
    GATCGTCCAG TTATGGGTGT AAATGGACTG GATGTGGCAG GACTTAGACC TTTTGATCTG
    GTTATTCCCT TCACCATTAA AAAAGGAGAG ATTACAGGAG AAGTGCGAAT GCCATCAGGG
    AAGGTGGCCA AGCCTGACAT CACTGATAAC AAAGATGGAA CAGTCACGGT GAGATATGCT
    CCCACTGAAG CTGGACTCCA TGAGATGGAT ATAAAGTATG ATGGCATGCA TATTCCAGGA
    AGCCCCCTAC AGTTTTATGT GGATTATGTA AACAGTGGCC ATGTTACTGC CTATGGCCCA
    GGCTTGATTC ATGGAACAGT GAATAAACCT GCTGTCTTCA CTGTGAACAC TAAGGATGCT
    GGAGAAGGTG GCTTGTCACT AGCTATTGAG GGCCCCTCCA AAGCAGAGAT CAGCTGCACA
    GATAACCAGG ATGGGACCTG CACAGTTTCT TATCTCCCTG TGCTCCCTGG AGACTACAAC
    ATCCTAGTCA AGTATAACAA CAAGCATATT CCGGGAAGTC CATTTGTTGC TAAAATAACA
    GGAGATGATT CTATGCGTAT GTCCCAGCTG AAGGTTGGGT CTGCAGCAGA CATCCCCCTG
    AACATTGTAG AGACAGACCT GAGCCAGCTG ACTGCTACAG TGACCTCTCC ATCTGGAAGA
    GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA AACGGCCATG TTGGCATCTC GTTTGTGCCA
    AAGGAGACAG GCGAACACAT TGTTAGCATA AAAAAAAATG GTATCCACAT CCCCAACAGC
    CCAATCACTG TAATGATCAG CCAATCCGAA ATCGGGGATG CCAGCCGAGT GATAGTGTCT
    GGTCAAGGTC TGACTGAAGG GAGGACATTT GAGCCCGCAG AATTCATCAT TGACACCAGG
    GAAGCAGGTT ATGGTGGACT TAGCCTTTCC ATCGAGGGGC CCAGCAAAGT GGATATCAAC
    ACAGAAGACT TGGAAGATGG GACATGCAAA GTTACTTACT GCCCAACTGA ACCTGGAAAT
    TATATTATCA ACATAAAGTT TGCTGATCAG CATGTTCCTG GCAGCCCCTT CTCTGTCAAG
    GTGACTGGGG AAGGAAGAAT GAAGGAGAGT ATCACTCGAA GACGCACCGC TCCATCTGTG
    GCAAACATTG GTAGCCAGTG TGATCTGAGC CTAAAAATAC CCGAGATCAG CATTCATGAC
    ATGACTGCCC AGGTCACTAG TCCCTCAGGG AAGATTCATG ATGCAGAAAT AATGGAAAGC
    GAGAATAACA CTTACTGCAT CCGATTTGTT CCTTCTGAGA TGGGCGTCCA CACAGTCAGT
    GTTAAGTACA AGGGACAGCA TGTGCCTGGA AGCCCCTTTC AGTTCACTGT TGGCCCCTTG
    GGTGAAGGAG GAGCCCACAA AGTACGAGCT GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT
    GGAACACCAG CGGAGTTCAG TATCTGGACC AGAGAGGCAG GAGCTGGTGG TCTATCAATT
    GCAGTGGAAG GTCCAAGCAA AGCAGAGATT GCATTTGAGG ACAGAAAGGA TGGTTCTTGT
    GGAGTGTCCT ACATTGTGCA GGAGCCTGGT GATTATGAGG TGTCTGTGAA GTTTAATGAT
    GAGCACATAC CAGACAGCCC CTTCGTGGTT CCAGTGGGTT CGACATCAGA TGATGCACGG
    AGACTTACTG TTTCTAGTCT TCAGGAGTCA GGGTTAAAGG TCAACCAGCC AGCCTCTTTT
    GCAGTAAGTC TAAATGGTGC CAAAGGAGTT CTAGATGCCA AGGTCCACAG CCCCTCAGGT
    GCCTTGGAGG AATGCTGTGT GACAGAAATT GATGAAGATA AATATGCTGT GCGCTTTATT
    CCCCGTGAAA ATGGTCTATA TATTGTCGAT GTAAAGTTTA ACGGCTCCCA TATACCTGGC
    AGTCCCTTCA AAATAAGAGT AGGGGAGCCA GGCCAAGCTG GGGATCCAGG AATGGTATCT
    GCATATGGCC CTGGGTTAGA AGGAGGCACC ACAGGCAGTG CTGCAGAGTT CATTGTCAAC
    ACCACAAAAG CAGGCCCTGG GGCACTCACA GTCACAATTG ATGGCCCGTC CAAAGTCAAA
    ATGGACTGCC AGGAATGTGC CGAGGGCTAT AAGGTCACCT ACACTCCTAT GGCTCCTGGA
    AGCTACCTGA TATCCATCAA GTACGGGGGA CCCTACCATA TTGTGGGAAG CCCATTCAAA
    GCAAAGGTTA CAGGTCCTCG CCTAGTGACC AGCCACAGCC TACATGAATC CTCTTCAGTC
    TTTGTTGATT CTGTCACCAA ATCTGAGGCC TTCACTCAGC ATGCCGCAGC TCCTAGGATA
    GCATCAGACG CCAGCAAAGT AGTGGCCAAA GGGCTAGGAC TCAACAAAGC CTTTGTAGGC
    CAGAAGAACT CTTTCACTGT GGACTGCAGT AAAGCTGGTA ACAACATGCT GCTTGTCGGA
    GTTCATGGAC CGAAAACCCC ATGTGAAGAG ATAGTGGTGA AGCACCTTGG GAACCGTCTC
    TACAACGTTA CGTACCTCCT GAAGGATAAG GGGGATTACA TCCTTGTAGT AAAATGGGGC
    GATGAGCACA TTCTGGGAAG CCCTTACCAT GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951799.1
    CDS176..8020
    Translation

    Target ORF information:

    RefSeq Version XM_018096310.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTACT
    GATCGTCCAG TTATGGGTGT AAATGGACTG GATGTGGCAG GACTTAGACC TTTTGATCTG
    GTTATTCCCT TCACCATTAA AAAAGGAGAG ATTACAGGAG AAGTGCGAAT GCCATCAGGG
    AAGGTGGCCA AGCCTGACAT CACTGATAAC AAAGATGGAA CAGTCACGGT GAGATATGCT
    CCCACTGAAG CTGGACTCCA TGAGATGGAT ATAAAGTATG ATGGCATGCA TATTCCAGGA
    AGCCCCCTAC AGTTTTATGT GGATTATGTA AACAGTGGCC ATGTTACTGC CTATGGCCCA
    GGCTTGATTC ATGGAACAGT GAATAAACCT GCTGTCTTCA CTGTGAACAC TAAGGATGCT
    GGAGAAGGTG GCTTGTCACT AGCTATTGAG GGCCCCTCCA AAGCAGAGAT CAGCTGCACA
    GATAACCAGG ATGGGACCTG CACAGTTTCT TATCTCCCTG TGCTCCCTGG AGACTACAAC
    ATCCTAGTCA AGTATAACAA CAAGCATATT CCGGGAAGTC CATTTGTTGC TAAAATAACA
    GGAGATGATT CTATGCGTAT GTCCCAGCTG AAGGTTGGGT CTGCAGCAGA CATCCCCCTG
    AACATTGTAG AGACAGACCT GAGCCAGCTG ACTGCTACAG TGACCTCTCC ATCTGGAAGA
    GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA AACGGCCATG TTGGCATCTC GTTTGTGCCA
    AAGGAGACAG GCGAACACAT TGTTAGCATA AAAAAAAATG GTATCCACAT CCCCAACAGC
    CCAATCACTG TAATGATCAG CCAATCCGAA ATCGGGGATG CCAGCCGAGT GATAGTGTCT
    GGTCAAGGTC TGACTGAAGG GAGGACATTT GAGCCCGCAG AATTCATCAT TGACACCAGG
    GAAGCAGGTT ATGGTGGACT TAGCCTTTCC ATCGAGGGGC CCAGCAAAGT GGATATCAAC
    ACAGAAGACT TGGAAGATGG GACATGCAAA GTTACTTACT GCCCAACTGA ACCTGGAAAT
    TATATTATCA ACATAAAGTT TGCTGATCAG CATGTTCCTG GCAGCCCCTT CTCTGTCAAG
    GTGACTGGGG AAGGAAGAAT GAAGGAGAGT ATCACTCGAA GACGCACCGC TCCATCTGTG
    GCAAACATTG GTAGCCAGTG TGATCTGAGC CTAAAAATAC CCGAGATCAG CATTCATGAC
    ATGACTGCCC AGGTCACTAG TCCCTCAGGG AAGATTCATG ATGCAGAAAT AATGGAAAGC
    GAGAATAACA CTTACTGCAT CCGATTTGTT CCTTCTGAGA TGGGCGTCCA CACAGTCAGT
    GTTAAGTACA AGGGACAGCA TGTGCCTGGA AGCCCCTTTC AGTTCACTGT TGGCCCCTTG
    GGTGAAGGAG GAGCCCACAA AGTACGAGCT GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT
    GGAACACCAG CGGAGTTCAG TATCTGGACC AGAGAGGCAG GAGCTGGTGG TCTATCAATT
    GCAGTGGAAG GTCCAAGCAA AGCAGAGATT GCATTTGAGG ACAGAAAGGA TGGTTCTTGT
    GGAGTGTCCT ACATTGTGCA GGAGCCTGGT GATTATGAGG TGTCTGTGAA GTTTAATGAT
    GAGCACATAC CAGACAGCCC CTTCGTGGTT CCAGTGGGTT CGACATCAGA TGATGCACGG
    AGACTTACTG TTTCTAGTCT TCAGGAGTCA GGGTTAAAGG TCAACCAGCC AGCCTCTTTT
    GCAGTAAGTC TAAATGGTGC CAAAGGAGTT CTAGATGCCA AGGTCCACAG CCCCTCAGGT
    GCCTTGGAGG AATGCTGTGT GACAGAAATT GATGAAGATA AATATGCTGT GCGCTTTATT
    CCCCGTGAAA ATGGTCTATA TATTGTCGAT GTAAAGTTTA ACGGCTCCCA TATACCTGGC
    AGTCCCTTCA AAATAAGAGT AGGGGAGCCA GGCCAAGCTG GGGATCCAGG AATGGTATCT
    GCATATGGCC CTGGGTTAGA AGGAGGCACC ACAGGCAGTG CTGCAGAGTT CATTGTCAAC
    ACCACAAAAG CAGGCCCTGG GGCACTCACA GTCACAATTG ATGGCCCGTC CAAAGTCAAA
    ATGGACTGCC AGGAATGTGC CGAGGGCTAT AAGGTCACCT ACACTCCTAT GGCTCCTGGA
    AGCTACCTGA TATCCATCAA GTACGGGGGA CCCTACCATA TTGTGGGAAG CCCATTCAAA
    GCAAAGGTTA CAGGTCCTCG CCTAGTGACC AGCCACAGCC TACATGAATC CTCTTCAGTC
    TTTGTTGATT CTGTCACCAA ATCTGAGGCC TTCACTCAGC ATGCCGCAGC TCCTAGGATA
    GCATCAGACG CCAGCAAAGT AGTGGCCAAA GGGCTAGGAC TCAACAAAGC CTTTGTAGGC
    CAGAAGAACT CTTTCACTGT GGACTGCAGT AAAGCTGGTA ACAACATGCT GCTTGTCGGA
    GTTCATGGAC CGAAAACCCC ATGTGAAGAG ATAGTGGTGA AGCACCTTGG GAACCGTCTC
    TACAACGTTA CGTACCTCCT GAAGGATAAG GGGGATTACA TCCTTGTAGT AAAATGGGGC
    GATGAGCACA TTCTGGGAAG CCCTTACCAT GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46056
    Clone ID Related Accession (Same CDS sequence) XM_018096311.1 , XM_018096311.2
    Accession Version XM_018096311.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    5881
    5941
    6001
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    6421
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    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTACTGATC GTCCAGTTAT GGGTGTAAAT
    GGACTGGATG TGGCAGGACT TAGACCTTTT GATCTGGTTA TTCCCTTCAC CATTAAAAAA
    GGAGAGATTA CAGGAGAAGT GCGAATGCCA TCAGGGAAGG TGGCCAAGCC TGACATCACT
    GATAACAAAG ATGGAACAGT CACGGTGAGA TATGCTCCCA CTGAAGCTGG ACTCCATGAG
    ATGGATATAA AGTATGATGG CATGCATATT CCAGGAAGCC CCCTACAGTT TTATGTGGAT
    TATGTAAACA GTGGCCATGT TACTGCCTAT GGCCCAGGCT TGATTCATGG AACAGTGAAT
    AAACCTGCTG TCTTCACTGT GAACACTAAG GATGCTGGAG AAGGTGGCTT GTCACTAGCT
    ATTGAGGGCC CCTCCAAAGC AGAGATCAGC TGCACAGATA ACCAGGATGG GACCTGCACA
    GTTTCTTATC TCCCTGTGCT CCCTGGAGAC TACAACATCC TAGTCAAGTA TAACAACAAG
    CATATTCCGG GAAGTCCATT TGTTGCTAAA ATAACAGGAG ATGATTCTAT GCGTATGTCC
    CAGCTGAAGG TTGGGTCTGC AGCAGACATC CCCCTGAACA TTGTAGAGAC AGACCTGAGC
    CAGCTGACTG CTACAGTGAC CTCTCCATCT GGAAGAGAGG AGCCATGTCT GCTCAAGAGG
    CTCAGAAACG GCCATGTTGG CATCTCGTTT GTGCCAAAGG AGACAGGCGA ACACATTGTT
    AGCATAAAAA AAAATGGTAT CCACATCCCC AACAGCCCAA TCACTGTAAT GATCAGCCAA
    TCCGAAATCG GGGATGCCAG CCGAGTGATA GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG
    ACATTTGAGC CCGCAGAATT CATCATTGAC ACCAGGGAAG CAGGTTATGG TGGACTTAGC
    CTTTCCATCG AGGGGCCCAG CAAAGTGGAT ATCAACACAG AAGACTTGGA AGATGGGACA
    TGCAAAGTTA CTTACTGCCC AACTGAACCT GGAAATTATA TTATCAACAT AAAGTTTGCT
    GATCAGCATG TTCCTGGCAG CCCCTTCTCT GTCAAGGTGA CTGGGGAAGG AAGAATGAAG
    GAGAGTATCA CTCGAAGACG CACCGCTCCA TCTGTGGCAA ACATTGGTAG CCAGTGTGAT
    CTGAGCCTAA AAATACCCGA GATCAGCATT CATGACATGA CTGCCCAGGT CACTAGTCCC
    TCAGGGAAGA TTCATGATGC AGAAATAATG GAAAGCGAGA ATAACACTTA CTGCATCCGA
    TTTGTTCCTT CTGAGATGGG CGTCCACACA GTCAGTGTTA AGTACAAGGG ACAGCATGTG
    CCTGGAAGCC CCTTTCAGTT CACTGTTGGC CCCTTGGGTG AAGGAGGAGC CCACAAAGTA
    CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG GAGGTTGGAA CACCAGCGGA GTTCAGTATC
    TGGACCAGAG AGGCAGGAGC TGGTGGTCTA TCAATTGCAG TGGAAGGTCC AAGCAAAGCA
    GAGATTGCAT TTGAGGACAG AAAGGATGGT TCTTGTGGAG TGTCCTACAT TGTGCAGGAG
    CCTGGTGATT ATGAGGTGTC TGTGAAGTTT AATGATGAGC ACATACCAGA CAGCCCCTTC
    GTGGTTCCAG TGGGTTCGAC ATCAGATGAT GCACGGAGAC TTACTGTTTC TAGTCTTCAG
    GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA
    GGAGTTCTAG ATGCCAAGGT CCACAGCCCC TCAGGTGCCT TGGAGGAATG CTGTGTGACA
    GAAATTGATG AAGATAAATA TGCTGTGCGC TTTATTCCCC GTGAAAATGG TCTATATATT
    GTCGATGTAA AGTTTAACGG CTCCCATATA CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG
    GAGCCAGGCC AAGCTGGGGA TCCAGGAATG GTATCTGCAT ATGGCCCTGG GTTAGAAGGA
    GGCACCACAG GCAGTGCTGC AGAGTTCATT GTCAACACCA CAAAAGCAGG CCCTGGGGCA
    CTCACAGTCA CAATTGATGG CCCGTCCAAA GTCAAAATGG ACTGCCAGGA ATGTGCCGAG
    GGCTATAAGG TCACCTACAC TCCTATGGCT CCTGGAAGCT ACCTGATATC CATCAAGTAC
    GGGGGACCCT ACCATATTGT GGGAAGCCCA TTCAAAGCAA AGGTTACAGG TCCTCGCCTA
    GTGACCAGCC ACAGCCTACA TGAATCCTCT TCAGTCTTTG TTGATTCTGT CACCAAATCT
    GAGGCCTTCA CTCAGCATGC CGCAGCTCCT AGGATAGCAT CAGACGCCAG CAAAGTAGTG
    GCCAAAGGGC TAGGACTCAA CAAAGCCTTT GTAGGCCAGA AGAACTCTTT CACTGTGGAC
    TGCAGTAAAG CTGGTAACAA CATGCTGCTT GTCGGAGTTC ATGGACCGAA AACCCCATGT
    GAAGAGATAG TGGTGAAGCA CCTTGGGAAC CGTCTCTACA ACGTTACGTA CCTCCTGAAG
    GATAAGGGGG ATTACATCCT TGTAGTAAAA TGGGGCGATG AGCACATTCT GGGAAGCCCT
    TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951800.1
    CDS183..8003
    Translation

    Target ORF information:

    RefSeq Version XM_018096311.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTACTGATC GTCCAGTTAT GGGTGTAAAT
    GGACTGGATG TGGCAGGACT TAGACCTTTT GATCTGGTTA TTCCCTTCAC CATTAAAAAA
    GGAGAGATTA CAGGAGAAGT GCGAATGCCA TCAGGGAAGG TGGCCAAGCC TGACATCACT
    GATAACAAAG ATGGAACAGT CACGGTGAGA TATGCTCCCA CTGAAGCTGG ACTCCATGAG
    ATGGATATAA AGTATGATGG CATGCATATT CCAGGAAGCC CCCTACAGTT TTATGTGGAT
    TATGTAAACA GTGGCCATGT TACTGCCTAT GGCCCAGGCT TGATTCATGG AACAGTGAAT
    AAACCTGCTG TCTTCACTGT GAACACTAAG GATGCTGGAG AAGGTGGCTT GTCACTAGCT
    ATTGAGGGCC CCTCCAAAGC AGAGATCAGC TGCACAGATA ACCAGGATGG GACCTGCACA
    GTTTCTTATC TCCCTGTGCT CCCTGGAGAC TACAACATCC TAGTCAAGTA TAACAACAAG
    CATATTCCGG GAAGTCCATT TGTTGCTAAA ATAACAGGAG ATGATTCTAT GCGTATGTCC
    CAGCTGAAGG TTGGGTCTGC AGCAGACATC CCCCTGAACA TTGTAGAGAC AGACCTGAGC
    CAGCTGACTG CTACAGTGAC CTCTCCATCT GGAAGAGAGG AGCCATGTCT GCTCAAGAGG
    CTCAGAAACG GCCATGTTGG CATCTCGTTT GTGCCAAAGG AGACAGGCGA ACACATTGTT
    AGCATAAAAA AAAATGGTAT CCACATCCCC AACAGCCCAA TCACTGTAAT GATCAGCCAA
    TCCGAAATCG GGGATGCCAG CCGAGTGATA GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG
    ACATTTGAGC CCGCAGAATT CATCATTGAC ACCAGGGAAG CAGGTTATGG TGGACTTAGC
    CTTTCCATCG AGGGGCCCAG CAAAGTGGAT ATCAACACAG AAGACTTGGA AGATGGGACA
    TGCAAAGTTA CTTACTGCCC AACTGAACCT GGAAATTATA TTATCAACAT AAAGTTTGCT
    GATCAGCATG TTCCTGGCAG CCCCTTCTCT GTCAAGGTGA CTGGGGAAGG AAGAATGAAG
    GAGAGTATCA CTCGAAGACG CACCGCTCCA TCTGTGGCAA ACATTGGTAG CCAGTGTGAT
    CTGAGCCTAA AAATACCCGA GATCAGCATT CATGACATGA CTGCCCAGGT CACTAGTCCC
    TCAGGGAAGA TTCATGATGC AGAAATAATG GAAAGCGAGA ATAACACTTA CTGCATCCGA
    TTTGTTCCTT CTGAGATGGG CGTCCACACA GTCAGTGTTA AGTACAAGGG ACAGCATGTG
    CCTGGAAGCC CCTTTCAGTT CACTGTTGGC CCCTTGGGTG AAGGAGGAGC CCACAAAGTA
    CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG GAGGTTGGAA CACCAGCGGA GTTCAGTATC
    TGGACCAGAG AGGCAGGAGC TGGTGGTCTA TCAATTGCAG TGGAAGGTCC AAGCAAAGCA
    GAGATTGCAT TTGAGGACAG AAAGGATGGT TCTTGTGGAG TGTCCTACAT TGTGCAGGAG
    CCTGGTGATT ATGAGGTGTC TGTGAAGTTT AATGATGAGC ACATACCAGA CAGCCCCTTC
    GTGGTTCCAG TGGGTTCGAC ATCAGATGAT GCACGGAGAC TTACTGTTTC TAGTCTTCAG
    GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA
    GGAGTTCTAG ATGCCAAGGT CCACAGCCCC TCAGGTGCCT TGGAGGAATG CTGTGTGACA
    GAAATTGATG AAGATAAATA TGCTGTGCGC TTTATTCCCC GTGAAAATGG TCTATATATT
    GTCGATGTAA AGTTTAACGG CTCCCATATA CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG
    GAGCCAGGCC AAGCTGGGGA TCCAGGAATG GTATCTGCAT ATGGCCCTGG GTTAGAAGGA
    GGCACCACAG GCAGTGCTGC AGAGTTCATT GTCAACACCA CAAAAGCAGG CCCTGGGGCA
    CTCACAGTCA CAATTGATGG CCCGTCCAAA GTCAAAATGG ACTGCCAGGA ATGTGCCGAG
    GGCTATAAGG TCACCTACAC TCCTATGGCT CCTGGAAGCT ACCTGATATC CATCAAGTAC
    GGGGGACCCT ACCATATTGT GGGAAGCCCA TTCAAAGCAA AGGTTACAGG TCCTCGCCTA
    GTGACCAGCC ACAGCCTACA TGAATCCTCT TCAGTCTTTG TTGATTCTGT CACCAAATCT
    GAGGCCTTCA CTCAGCATGC CGCAGCTCCT AGGATAGCAT CAGACGCCAG CAAAGTAGTG
    GCCAAAGGGC TAGGACTCAA CAAAGCCTTT GTAGGCCAGA AGAACTCTTT CACTGTGGAC
    TGCAGTAAAG CTGGTAACAA CATGCTGCTT GTCGGAGTTC ATGGACCGAA AACCCCATGT
    GAAGAGATAG TGGTGAAGCA CCTTGGGAAC CGTCTCTACA ACGTTACGTA CCTCCTGAAG
    GATAAGGGGG ATTACATCCT TGTAGTAAAA TGGGGCGATG AGCACATTCT GGGAAGCCCT
    TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46056
    Clone ID Related Accession (Same CDS sequence) XM_018096311.1 , XM_018096311.2
    Accession Version XM_018096311.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTACTGATC GTCCAGTTAT GGGTGTAAAT
    GGACTGGATG TGGCAGGACT TAGACCTTTT GATCTGGTTA TTCCCTTCAC CATTAAAAAA
    GGAGAGATTA CAGGAGAAGT GCGAATGCCA TCAGGGAAGG TGGCCAAGCC TGACATCACT
    GATAACAAAG ATGGAACAGT CACGGTGAGA TATGCTCCCA CTGAAGCTGG ACTCCATGAG
    ATGGATATAA AGTATGATGG CATGCATATT CCAGGAAGCC CCCTACAGTT TTATGTGGAT
    TATGTAAACA GTGGCCATGT TACTGCCTAT GGCCCAGGCT TGATTCATGG AACAGTGAAT
    AAACCTGCTG TCTTCACTGT GAACACTAAG GATGCTGGAG AAGGTGGCTT GTCACTAGCT
    ATTGAGGGCC CCTCCAAAGC AGAGATCAGC TGCACAGATA ACCAGGATGG GACCTGCACA
    GTTTCTTATC TCCCTGTGCT CCCTGGAGAC TACAACATCC TAGTCAAGTA TAACAACAAG
    CATATTCCGG GAAGTCCATT TGTTGCTAAA ATAACAGGAG ATGATTCTAT GCGTATGTCC
    CAGCTGAAGG TTGGGTCTGC AGCAGACATC CCCCTGAACA TTGTAGAGAC AGACCTGAGC
    CAGCTGACTG CTACAGTGAC CTCTCCATCT GGAAGAGAGG AGCCATGTCT GCTCAAGAGG
    CTCAGAAACG GCCATGTTGG CATCTCGTTT GTGCCAAAGG AGACAGGCGA ACACATTGTT
    AGCATAAAAA AAAATGGTAT CCACATCCCC AACAGCCCAA TCACTGTAAT GATCAGCCAA
    TCCGAAATCG GGGATGCCAG CCGAGTGATA GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG
    ACATTTGAGC CCGCAGAATT CATCATTGAC ACCAGGGAAG CAGGTTATGG TGGACTTAGC
    CTTTCCATCG AGGGGCCCAG CAAAGTGGAT ATCAACACAG AAGACTTGGA AGATGGGACA
    TGCAAAGTTA CTTACTGCCC AACTGAACCT GGAAATTATA TTATCAACAT AAAGTTTGCT
    GATCAGCATG TTCCTGGCAG CCCCTTCTCT GTCAAGGTGA CTGGGGAAGG AAGAATGAAG
    GAGAGTATCA CTCGAAGACG CACCGCTCCA TCTGTGGCAA ACATTGGTAG CCAGTGTGAT
    CTGAGCCTAA AAATACCCGA GATCAGCATT CATGACATGA CTGCCCAGGT CACTAGTCCC
    TCAGGGAAGA TTCATGATGC AGAAATAATG GAAAGCGAGA ATAACACTTA CTGCATCCGA
    TTTGTTCCTT CTGAGATGGG CGTCCACACA GTCAGTGTTA AGTACAAGGG ACAGCATGTG
    CCTGGAAGCC CCTTTCAGTT CACTGTTGGC CCCTTGGGTG AAGGAGGAGC CCACAAAGTA
    CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG GAGGTTGGAA CACCAGCGGA GTTCAGTATC
    TGGACCAGAG AGGCAGGAGC TGGTGGTCTA TCAATTGCAG TGGAAGGTCC AAGCAAAGCA
    GAGATTGCAT TTGAGGACAG AAAGGATGGT TCTTGTGGAG TGTCCTACAT TGTGCAGGAG
    CCTGGTGATT ATGAGGTGTC TGTGAAGTTT AATGATGAGC ACATACCAGA CAGCCCCTTC
    GTGGTTCCAG TGGGTTCGAC ATCAGATGAT GCACGGAGAC TTACTGTTTC TAGTCTTCAG
    GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA
    GGAGTTCTAG ATGCCAAGGT CCACAGCCCC TCAGGTGCCT TGGAGGAATG CTGTGTGACA
    GAAATTGATG AAGATAAATA TGCTGTGCGC TTTATTCCCC GTGAAAATGG TCTATATATT
    GTCGATGTAA AGTTTAACGG CTCCCATATA CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG
    GAGCCAGGCC AAGCTGGGGA TCCAGGAATG GTATCTGCAT ATGGCCCTGG GTTAGAAGGA
    GGCACCACAG GCAGTGCTGC AGAGTTCATT GTCAACACCA CAAAAGCAGG CCCTGGGGCA
    CTCACAGTCA CAATTGATGG CCCGTCCAAA GTCAAAATGG ACTGCCAGGA ATGTGCCGAG
    GGCTATAAGG TCACCTACAC TCCTATGGCT CCTGGAAGCT ACCTGATATC CATCAAGTAC
    GGGGGACCCT ACCATATTGT GGGAAGCCCA TTCAAAGCAA AGGTTACAGG TCCTCGCCTA
    GTGACCAGCC ACAGCCTACA TGAATCCTCT TCAGTCTTTG TTGATTCTGT CACCAAATCT
    GAGGCCTTCA CTCAGCATGC CGCAGCTCCT AGGATAGCAT CAGACGCCAG CAAAGTAGTG
    GCCAAAGGGC TAGGACTCAA CAAAGCCTTT GTAGGCCAGA AGAACTCTTT CACTGTGGAC
    TGCAGTAAAG CTGGTAACAA CATGCTGCTT GTCGGAGTTC ATGGACCGAA AACCCCATGT
    GAAGAGATAG TGGTGAAGCA CCTTGGGAAC CGTCTCTACA ACGTTACGTA CCTCCTGAAG
    GATAAGGGGG ATTACATCCT TGTAGTAAAA TGGGGCGATG AGCACATTCT GGGAAGCCCT
    TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951800.1
    CDS176..7996
    Translation

    Target ORF information:

    RefSeq Version XM_018096311.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096311.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTACTGATC GTCCAGTTAT GGGTGTAAAT
    GGACTGGATG TGGCAGGACT TAGACCTTTT GATCTGGTTA TTCCCTTCAC CATTAAAAAA
    GGAGAGATTA CAGGAGAAGT GCGAATGCCA TCAGGGAAGG TGGCCAAGCC TGACATCACT
    GATAACAAAG ATGGAACAGT CACGGTGAGA TATGCTCCCA CTGAAGCTGG ACTCCATGAG
    ATGGATATAA AGTATGATGG CATGCATATT CCAGGAAGCC CCCTACAGTT TTATGTGGAT
    TATGTAAACA GTGGCCATGT TACTGCCTAT GGCCCAGGCT TGATTCATGG AACAGTGAAT
    AAACCTGCTG TCTTCACTGT GAACACTAAG GATGCTGGAG AAGGTGGCTT GTCACTAGCT
    ATTGAGGGCC CCTCCAAAGC AGAGATCAGC TGCACAGATA ACCAGGATGG GACCTGCACA
    GTTTCTTATC TCCCTGTGCT CCCTGGAGAC TACAACATCC TAGTCAAGTA TAACAACAAG
    CATATTCCGG GAAGTCCATT TGTTGCTAAA ATAACAGGAG ATGATTCTAT GCGTATGTCC
    CAGCTGAAGG TTGGGTCTGC AGCAGACATC CCCCTGAACA TTGTAGAGAC AGACCTGAGC
    CAGCTGACTG CTACAGTGAC CTCTCCATCT GGAAGAGAGG AGCCATGTCT GCTCAAGAGG
    CTCAGAAACG GCCATGTTGG CATCTCGTTT GTGCCAAAGG AGACAGGCGA ACACATTGTT
    AGCATAAAAA AAAATGGTAT CCACATCCCC AACAGCCCAA TCACTGTAAT GATCAGCCAA
    TCCGAAATCG GGGATGCCAG CCGAGTGATA GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG
    ACATTTGAGC CCGCAGAATT CATCATTGAC ACCAGGGAAG CAGGTTATGG TGGACTTAGC
    CTTTCCATCG AGGGGCCCAG CAAAGTGGAT ATCAACACAG AAGACTTGGA AGATGGGACA
    TGCAAAGTTA CTTACTGCCC AACTGAACCT GGAAATTATA TTATCAACAT AAAGTTTGCT
    GATCAGCATG TTCCTGGCAG CCCCTTCTCT GTCAAGGTGA CTGGGGAAGG AAGAATGAAG
    GAGAGTATCA CTCGAAGACG CACCGCTCCA TCTGTGGCAA ACATTGGTAG CCAGTGTGAT
    CTGAGCCTAA AAATACCCGA GATCAGCATT CATGACATGA CTGCCCAGGT CACTAGTCCC
    TCAGGGAAGA TTCATGATGC AGAAATAATG GAAAGCGAGA ATAACACTTA CTGCATCCGA
    TTTGTTCCTT CTGAGATGGG CGTCCACACA GTCAGTGTTA AGTACAAGGG ACAGCATGTG
    CCTGGAAGCC CCTTTCAGTT CACTGTTGGC CCCTTGGGTG AAGGAGGAGC CCACAAAGTA
    CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG GAGGTTGGAA CACCAGCGGA GTTCAGTATC
    TGGACCAGAG AGGCAGGAGC TGGTGGTCTA TCAATTGCAG TGGAAGGTCC AAGCAAAGCA
    GAGATTGCAT TTGAGGACAG AAAGGATGGT TCTTGTGGAG TGTCCTACAT TGTGCAGGAG
    CCTGGTGATT ATGAGGTGTC TGTGAAGTTT AATGATGAGC ACATACCAGA CAGCCCCTTC
    GTGGTTCCAG TGGGTTCGAC ATCAGATGAT GCACGGAGAC TTACTGTTTC TAGTCTTCAG
    GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA
    GGAGTTCTAG ATGCCAAGGT CCACAGCCCC TCAGGTGCCT TGGAGGAATG CTGTGTGACA
    GAAATTGATG AAGATAAATA TGCTGTGCGC TTTATTCCCC GTGAAAATGG TCTATATATT
    GTCGATGTAA AGTTTAACGG CTCCCATATA CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG
    GAGCCAGGCC AAGCTGGGGA TCCAGGAATG GTATCTGCAT ATGGCCCTGG GTTAGAAGGA
    GGCACCACAG GCAGTGCTGC AGAGTTCATT GTCAACACCA CAAAAGCAGG CCCTGGGGCA
    CTCACAGTCA CAATTGATGG CCCGTCCAAA GTCAAAATGG ACTGCCAGGA ATGTGCCGAG
    GGCTATAAGG TCACCTACAC TCCTATGGCT CCTGGAAGCT ACCTGATATC CATCAAGTAC
    GGGGGACCCT ACCATATTGT GGGAAGCCCA TTCAAAGCAA AGGTTACAGG TCCTCGCCTA
    GTGACCAGCC ACAGCCTACA TGAATCCTCT TCAGTCTTTG TTGATTCTGT CACCAAATCT
    GAGGCCTTCA CTCAGCATGC CGCAGCTCCT AGGATAGCAT CAGACGCCAG CAAAGTAGTG
    GCCAAAGGGC TAGGACTCAA CAAAGCCTTT GTAGGCCAGA AGAACTCTTT CACTGTGGAC
    TGCAGTAAAG CTGGTAACAA CATGCTGCTT GTCGGAGTTC ATGGACCGAA AACCCCATGT
    GAAGAGATAG TGGTGAAGCA CCTTGGGAAC CGTCTCTACA ACGTTACGTA CCTCCTGAAG
    GATAAGGGGG ATTACATCCT TGTAGTAAAA TGGGGCGATG AGCACATTCT GGGAAGCCCT
    TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29245
    Clone ID Related Accession (Same CDS sequence) XM_012968610.2 , NM_001374713.1
    Accession Version XM_012968610.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTCTG
    GACGGCGAGC AAATGATGCC TCAGCAGATG GTTCAACAGC AGTTAGCCCC ACCATACGCT
    TACCCGGGCA TGCAACAACC CTGGGCTACT GATCGTCCAG TTATGGGTGT AAATGGACTG
    GATGTGGCAG GACTTAGACC TTTTGATCTG GTTATTCCCT TCACCATTAA AAAAGGAGAG
    ATTACAGGAG AAGTGCGAAT GCCATCAGGG AAGGTGGCCA AGCCTGACAT CACTGATAAC
    AAAGATGGAA CAGTCACGGT GAGATATGCT CCCACTGAAG CTGGACTCCA TGAGATGGAT
    ATAAAGTATG ATGGCATGCA TATTCCAGGA AGCCCCCTAC AGTTTTATGT GGATTATGTA
    AACAGTGGCC ATGTTACTGC CTATGGCCCA GGCTTGATTC ATGGAACAGT GAATAAACCT
    GCTGTCTTCA CTGTGAACAC TAAGGATGCT GGAGAAGGTG GCTTGTCACT AGCTATTGAG
    GGCCCCTCCA AAGCAGAGAT CAGCTGCACA GATAACCAGG ATGGGACCTG CACAGTTTCT
    TATCTCCCTG TGCTCCCTGG AGACTACAAC ATCCTAGTCA AGTATAACAA CAAGCATATT
    CCGGGAAGTC CATTTGTTGC TAAAATAACA GGAGATGATT CTATGCGTAT GTCCCAGCTG
    AAGGTTGGGT CTGCAGCAGA CATCCCCCTG AACATTGTAG AGACAGACCT GAGCCAGCTG
    ACTGCTACAG TGACCTCTCC ATCTGGAAGA GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA
    AACGGCCATG TTGGCATCTC GTTTGTGCCA AAGGAGACAG GCGAACACAT TGTTAGCATA
    AAAAAAAATG GTATCCACAT CCCCAACAGC CCAATCACTG TAATGATCAG CCAATCCGAA
    ATCGGGGATG CCAGCCGAGT GATAGTGTCT GGTCAAGGTC TGACTGAAGG GAGGACATTT
    GAGCCCGCAG AATTCATCAT TGACACCAGG GAAGCAGGTT ATGGTGGACT TAGCCTTTCC
    ATCGAGGGGC CCAGCAAAGT GGATATCAAC ACAGAAGACT TGGAAGATGG GACATGCAAA
    GTTACTTACT GCCCAACTGA ACCTGGAAAT TATATTATCA ACATAAAGTT TGCTGATCAG
    CATGTTCCTG GCAGCCCCTT CTCTGTCAAG GTGACTGGGG AAGGAAGAAT GAAGGAGAGT
    ATCACTCGAA GACGCACCGC TCCATCTGTG GCAAACATTG GTAGCCAGTG TGATCTGAGC
    CTAAAAATAC CCGAGATCAG CATTCATGAC ATGACTGCCC AGGTCACTAG TCCCTCAGGG
    AAGATTCATG ATGCAGAAAT AATGGAAAGC GAGAATAACA CTTACTGCAT CCGATTTGTT
    CCTTCTGAGA TGGGCGTCCA CACAGTCAGT GTTAAGTACA AGGGACAGCA TGTGCCTGGA
    AGCCCCTTTC AGTTCACTGT TGGCCCCTTG GGTGAAGGAG GAGCCCACAA AGTACGAGCT
    GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT GGAACACCAG CGGAGTTCAG TATCTGGACC
    AGAGAGGCAG GAGCTGGTGG TCTATCAATT GCAGTGGAAG GTCCAAGCAA AGCAGAGATT
    GCATTTGAGG ACAGAAAGGA TGGTTCTTGT GGAGTGTCCT ACATTGTGCA GGAGCCTGGT
    GATTATGAGG TGTCTGTGAA GTTTAATGAT GAGCACATAC CAGACAGCCC CTTCGTGGTT
    CCAGTGGGTT CGACATCAGA TGATGCACGG AGACTTACTG TTTCTAGTCT TCAGGAGTCA
    GGGTTAAAGG TCAACCAGCC AGCCTCTTTT GCAGTAAGTC TAAATGGTGC CAAAGGAGTT
    CTAGATGCCA AGGTCCACAG CCCCTCAGGT GCCTTGGAGG AATGCTGTGT GACAGAAATT
    GATGAAGATA AATATGCTGT GCGCTTTATT CCCCGTGAAA ATGGTCTATA TATTGTCGAT
    GTAAAGTTTA ACGGCTCCCA TATACCTGGC AGTCCCTTCA AAATAAGAGT AGGGGAGCCA
    GGCCAAGCTG GGGATCCAGG AATGGTATCT GCATATGGCC CTGGGTTAGA AGGAGGCACC
    ACAGGCAGTG CTGCAGAGTT CATTGTCAAC ACCACAAAAG CAGGCCCTGG GGCACTCACA
    GTCACAATTG ATGGCCCGTC CAAAGTCAAA ATGGACTGCC AGGAATGTGC CGAGGGCTAT
    AAGGTCACCT ACACTCCTAT GGCTCCTGGA AGCTACCTGA TATCCATCAA GTACGGGGGA
    CCCTACCATA TTGTGGGAAG CCCATTCAAA GCAAAGGTTA CAGGTCCTCG CCTAGTGACC
    AGCCACAGCC TACATGAATC CTCTTCAGTC TTTGTTGATT CTGTCACCAA ATCTGAGGCC
    TTCACTCAGC ATGCCGCAGC TCCTAGGATA GCATCAGACG CCAGCAAAGT AGTGGCCAAA
    GGGCTAGGAC TCAACAAAGC CTTTGTAGGC CAGAAGAACT CTTTCACTGT GGACTGCAGT
    AAAGCTGGTA ACAACATGCT GCTTGTCGGA GTTCATGGAC CGAAAACCCC ATGTGAAGAG
    ATAGTGGTGA AGCACCTTGG GAACCGTCTC TACAACGTTA CGTACCTCCT GAAGGATAAG
    GGGGATTACA TCCTTGTAGT AAAATGGGGC GATGAGCACA TTCTGGGAAG CCCTTACCAT
    GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824064.1
    CDS182..8116
    Translation

    Target ORF information:

    RefSeq Version XM_012968610.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968610.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGTGTCAATC GGAGGTCACG GGCTAGGTGC CGGCATTGGT
    CCAACTATAC AGATTGGCGA AGAAACCGTA ATAACAGTTG ATGCCAAAGC AGCTGGGAAG
    GGTAAAGTGA CCTGCACTGT GTGCACTCCA GATGGCACAG AAGTAGATGT TGATGTGGTA
    GAGAATGAAG ATGGCACCTT TGACATATTC TATACAGCTC CACAACCTGG AAAATATGTT
    ATTTGTGTGC GCTTCGGTGG GGAACACATT CCCAACAGTC CTTTCCAGGT CACGGCTCTG
    GACGGCGAGC AAATGATGCC TCAGCAGATG GTTCAACAGC AGTTAGCCCC ACCATACGCT
    TACCCGGGCA TGCAACAACC CTGGGCTACT GATCGTCCAG TTATGGGTGT AAATGGACTG
    GATGTGGCAG GACTTAGACC TTTTGATCTG GTTATTCCCT TCACCATTAA AAAAGGAGAG
    ATTACAGGAG AAGTGCGAAT GCCATCAGGG AAGGTGGCCA AGCCTGACAT CACTGATAAC
    AAAGATGGAA CAGTCACGGT GAGATATGCT CCCACTGAAG CTGGACTCCA TGAGATGGAT
    ATAAAGTATG ATGGCATGCA TATTCCAGGA AGCCCCCTAC AGTTTTATGT GGATTATGTA
    AACAGTGGCC ATGTTACTGC CTATGGCCCA GGCTTGATTC ATGGAACAGT GAATAAACCT
    GCTGTCTTCA CTGTGAACAC TAAGGATGCT GGAGAAGGTG GCTTGTCACT AGCTATTGAG
    GGCCCCTCCA AAGCAGAGAT CAGCTGCACA GATAACCAGG ATGGGACCTG CACAGTTTCT
    TATCTCCCTG TGCTCCCTGG AGACTACAAC ATCCTAGTCA AGTATAACAA CAAGCATATT
    CCGGGAAGTC CATTTGTTGC TAAAATAACA GGAGATGATT CTATGCGTAT GTCCCAGCTG
    AAGGTTGGGT CTGCAGCAGA CATCCCCCTG AACATTGTAG AGACAGACCT GAGCCAGCTG
    ACTGCTACAG TGACCTCTCC ATCTGGAAGA GAGGAGCCAT GTCTGCTCAA GAGGCTCAGA
    AACGGCCATG TTGGCATCTC GTTTGTGCCA AAGGAGACAG GCGAACACAT TGTTAGCATA
    AAAAAAAATG GTATCCACAT CCCCAACAGC CCAATCACTG TAATGATCAG CCAATCCGAA
    ATCGGGGATG CCAGCCGAGT GATAGTGTCT GGTCAAGGTC TGACTGAAGG GAGGACATTT
    GAGCCCGCAG AATTCATCAT TGACACCAGG GAAGCAGGTT ATGGTGGACT TAGCCTTTCC
    ATCGAGGGGC CCAGCAAAGT GGATATCAAC ACAGAAGACT TGGAAGATGG GACATGCAAA
    GTTACTTACT GCCCAACTGA ACCTGGAAAT TATATTATCA ACATAAAGTT TGCTGATCAG
    CATGTTCCTG GCAGCCCCTT CTCTGTCAAG GTGACTGGGG AAGGAAGAAT GAAGGAGAGT
    ATCACTCGAA GACGCACCGC TCCATCTGTG GCAAACATTG GTAGCCAGTG TGATCTGAGC
    CTAAAAATAC CCGAGATCAG CATTCATGAC ATGACTGCCC AGGTCACTAG TCCCTCAGGG
    AAGATTCATG ATGCAGAAAT AATGGAAAGC GAGAATAACA CTTACTGCAT CCGATTTGTT
    CCTTCTGAGA TGGGCGTCCA CACAGTCAGT GTTAAGTACA AGGGACAGCA TGTGCCTGGA
    AGCCCCTTTC AGTTCACTGT TGGCCCCTTG GGTGAAGGAG GAGCCCACAA AGTACGAGCT
    GGGGGTCCTG GCCTGGAAAG AGCGGAGGTT GGAACACCAG CGGAGTTCAG TATCTGGACC
    AGAGAGGCAG GAGCTGGTGG TCTATCAATT GCAGTGGAAG GTCCAAGCAA AGCAGAGATT
    GCATTTGAGG ACAGAAAGGA TGGTTCTTGT GGAGTGTCCT ACATTGTGCA GGAGCCTGGT
    GATTATGAGG TGTCTGTGAA GTTTAATGAT GAGCACATAC CAGACAGCCC CTTCGTGGTT
    CCAGTGGGTT CGACATCAGA TGATGCACGG AGACTTACTG TTTCTAGTCT TCAGGAGTCA
    GGGTTAAAGG TCAACCAGCC AGCCTCTTTT GCAGTAAGTC TAAATGGTGC CAAAGGAGTT
    CTAGATGCCA AGGTCCACAG CCCCTCAGGT GCCTTGGAGG AATGCTGTGT GACAGAAATT
    GATGAAGATA AATATGCTGT GCGCTTTATT CCCCGTGAAA ATGGTCTATA TATTGTCGAT
    GTAAAGTTTA ACGGCTCCCA TATACCTGGC AGTCCCTTCA AAATAAGAGT AGGGGAGCCA
    GGCCAAGCTG GGGATCCAGG AATGGTATCT GCATATGGCC CTGGGTTAGA AGGAGGCACC
    ACAGGCAGTG CTGCAGAGTT CATTGTCAAC ACCACAAAAG CAGGCCCTGG GGCACTCACA
    GTCACAATTG ATGGCCCGTC CAAAGTCAAA ATGGACTGCC AGGAATGTGC CGAGGGCTAT
    AAGGTCACCT ACACTCCTAT GGCTCCTGGA AGCTACCTGA TATCCATCAA GTACGGGGGA
    CCCTACCATA TTGTGGGAAG CCCATTCAAA GCAAAGGTTA CAGGTCCTCG CCTAGTGACC
    AGCCACAGCC TACATGAATC CTCTTCAGTC TTTGTTGATT CTGTCACCAA ATCTGAGGCC
    TTCACTCAGC ATGCCGCAGC TCCTAGGATA GCATCAGACG CCAGCAAAGT AGTGGCCAAA
    GGGCTAGGAC TCAACAAAGC CTTTGTAGGC CAGAAGAACT CTTTCACTGT GGACTGCAGT
    AAAGCTGGTA ACAACATGCT GCTTGTCGGA GTTCATGGAC CGAAAACCCC ATGTGAAGAG
    ATAGTGGTGA AGCACCTTGG GAACCGTCTC TACAACGTTA CGTACCTCCT GAAGGATAAG
    GGGGATTACA TCCTTGTAGT AAAATGGGGC GATGAGCACA TTCTGGGAAG CCCTTACCAT
    GTGTCTGTGC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29246
    Clone ID Related Accession (Same CDS sequence) XM_012968611.2 , XM_012968611.3
    Accession Version XM_012968611.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968611.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824065.1
    CDS182..8092
    Translation

    Target ORF information:

    RefSeq Version XM_012968611.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968611.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGAGCAGCG CACACAGTAG GCTCAGTACC CAGAGCGGAG GCGCAGGAGG AGCGGTGCCC 
    GGGGAGGCAG CAGAGATGCC GGCCACAGAG AAGGACCTGG CGGAGGACGC TCCGTGGAAG
    AAGATCCAGC AGAACACTTT CACTCGCTGG TGCAACGAGC ACCTGAAATG CGTCAACAAG
    CGCATCGCAA ACCTGCAGGT GGACCTGGGC GACGGGCTGC GACTCATCGC CTTGCTGGAG
    GTGCTGAGCC AGAAGAAGAT GTACCGCAAG TACAACCAGA GACCCACCTT CCGCCAGATG
    CAGCTGGAGA ACGTGTCCGT GGCCCTCGAA TTCCTGGACC GGGAGAACAT CAAGCTCGTA
    TCCATAGACA GCAAAGCAAT TGTAGATGGG AACCTCAAAC TTATCCTGGG TCTTATCTGG
    ACCCTCATTC TGCACTATTC CATCTCCATG CCCATGTGGG ATGAGGAAGA GGATGAGGAG
    GCAAAGAAAC AGACCCCAAA GCAAAGGCTG CTGGGATGGA TTCAAAACAA GCTGCCTCAT
    CTCCCTGTCA CCAACTTCAG CCGGGACTGG CAGAGTGGCC GAGCTCTGGG TGCCCTTGTG
    GACAGCTGCG CTCCTGGTCT TTGCCCAGAT TGGGACTCCT GGGATGCCAC CAAGCCTGTG
    GATAATGCAC GTGAGGCCAT GCAGCAGGCA GATGACTGGC TGGGAATACC ACAGGTGATC
    ACCCCTGAGG AAATAGTCGA CCCCAATGTA GATGAACACT CAGTCATGAC CTACCTGTCA
    CAGTTCCCCA AGGCCAAGCT GAAACCTGGT GCTCCACTAA GGCCAAAACT GAATCCCAAA
    AAGGCAAGAG CTTATGGACC AGGTATTGAA CCAACTGGAA ACATGGTGAA AAAGCGTGCA
    GAATTTACAG TTGAGACCAT CAGCGCTGGA ATGGGGGAGG TGCTGGTATA TGTAGAAGAT
    CCCGCAGGAC ACAGAGAAGA GGCCAAAGTT ACAGCAAACA ATGATAAGAA CCGAACCTTT
    TCTGTCTACT ATATCCCCAA AGTAACTGGG TTGCATAAGG TAACTGTCCT GTTTGCTGGT
    CAGCATATTG CAAAGAGTCC CTTTGAAGTA AATGTGGACA AATCCCATGG TGATGCCAGC
    AAGGTTACCG CCCAAGGTCC TGGTCTGGAA CCCAGTGGAA ACATTGCGAA TAAGACCACT
    TACTTTGAGA TCTTTACTGC AGGTGCTGGG GTAGGAGAAA TAGAAGTGAC CATTGTTGAC
    CCCAACGGGA AGAAAGATAC AGCAGATGTG CAGCTGGAAG ATAAAGGCAG CAGCACGTAC
    CGCTGCACCT ACAAACCGAC CATGGAAGGA GTATATACAA TCTACATAAC CTTTGCTGGT
    GTTCAGATCC CAAAAAGTCC ATACACTGTT ACTATTGGAC AAGCTTGCAA TCCCGCCGCC
    TGCCGTGCTG TGGGCCGTGG CTTGCAGCCA AAGGGTGTAC GCGTGAAGGA GACGGCGGAT
    TTTAAGGTTT ATACTAAAGG CGCAGGAAGC GGTGAACTTA AAGTTGTGGT CAAGGGACCA
    AAGGGTACAG AGGAACGTGT CAAGCAGAAG GACCTAGGAG ATGGTGTGTA TGCCTTTGAG
    TACTATCCAG CTACACCTGG GAACTATATT GTTACCATCA CATGGGGCGG ACAGAATATC
    CCCAGAAGTC CATTTGAGGT AAAAGTTGGC ACTGAATGTG GACAACAAAG AGTTCGAGCA
    TGGGGCCCTG GTCTTGAAGG AGGGGTGGTT GGAAAATCAG CTGACTTTGT TGTAGAAGCA
    ATCGGTGATG ATGTTGGGAC TCTCGGGTTT TCTGTGGAAG GTCCATCTCA AGCTAAGATC
    GAGTGTGACG ATAAAGGAGA TGGGTCCTGT GATGTGCGCT ACTGGCCACT GGAACCTGGA
    GAGTATGCGG TGCATGTTCT GTGCAACAAT GAGGACATCA AACTGAGTCC TTTCATGGCT
    GATATCAAAC CAGCCCCCAA AGACTTCTAC CCAGAGAAGG TCAAGGCCTA TGGTCCTGGG
    CTAGAGAAGA CTGGGTTGGC AATAAACAAA CCAGCAGAGT TCACTGTTGA TGCCAAACTT
    GGAGGAAAGG CTCCTCTCAA GGTGTCTGTG CAGGATTCTG AAGGCACTCC AGTGGATGTC
    ACAACCAAAG ATAATGGAAA TGGCACATAC AGCTGCAGTT ATTTGCCAAA GAAACCTCTG
    AAACACACAG CTGTGGTGTC TTGGGGTGGG GTGAACATTC CCAACAGTCC TTTCAGGATG
    ACTATTGGTG CTGGAAGCCA CCCTAACAAG GTGAAGGTTT ATGGACCTGG TGTTGCTAAG
    ACTGGCCTGA AAGCTCATGA ACCAACCTAC TTTACTGTGG ATTGCACAGA AGCTGGTCAA
    GGTGATGTGA GTATTGGAAT TAAATGTGCC CCAGGAGTTG TGGGCCCGGC TGAGGCGGAC
    ATAGACTTTG ACATTATCCG CAATGATAAT GATACATTCA CCGTGAAGTA CACTCCTCCT
    GGAGCTGGGA GCTACACCAT CATGGTCCTG TTTGCTGACC AGGCTACTCC AATGAGTCCA
    TTCCGTATCA AGGTTGATCC ATCACATGAT GCAAGTAAAG TTAAGGCAGA AGGACCTGGA
    CTAAATAGAA GTGGTGTAGA AATTGGAAAA CCAACTCATT TCACAGTTAA CACCAAGGCT
    GCTGGAAAAG CCAACCTAGA CGTGCAGTTT ACAGGTCCAG CCAAGGGAGA GGCTGTGAAG
    GACTTTGATG TTATTGACAA CCACGATAAC ACCTACACCG TAAAATACAC TCCAGTGCAA
    CAGGGTAACA TGGGTGTAAA TGTGACCTAT GGTGGTGACC ATATTCCAAA GAGTCCCTTC
    ACCGTTGGCA TTGCCCCAAC TTTGGACCTC AGTAAAATCA AAGTTTCTGG ACTTGGTGAC
    AAGGTGGAGG TCGGGAAGGA TCAGGAGTTC ACAGTAAAAT CCAGGGGTGC TGGAGGACAA
    GGCAAAGTGG CTTGCAAGAT CACAGGACCA TCCAACAAGT CTGTTCCTTG CAAGGTGGAA
    CCTGGTCTGA GCCCAGATAA CAGCACTGTC AAATTCATCC CTAGGGAGGA AGGCCCATAT
    GAGGTAGACG TGACGTACGA CGGAGTGCCT ATACCTGGTA GCCCATTTCC CGTTGAGGCA
    GTTCCTTCAA CAAATCCTTC AAAGGTCAAA GCTTTTGGAC CTGGTCTAAA AGGAGGCAGT
    GTTGGTTCCT CTGCACCCTT TACTATTGAC ACTAAAGGTG CAGGCCAAGG TGGGCTTGGT
    CTCACTGTTG AAGGTCCATG TGAAGCCAAA ATAGAATGTC TAGACAATGG AGATGGAACC
    TGTTCTGTCT CTTACTTGCC TACAGAACGT GGTGACTATA ATATCAATAT CTTGTTTGCT
    GATACACATA TCCCTGGATC ACCTTTCAAG GCTAAGGTTG AGCCATGTTT CGATCCTTCA
    AAGGTGACCT GTTCTGGTCC TGGTCTGGAA CATGCTCAGG TTGGAGAAGC TGGTAAATTT
    AATGTGGACT GTTCAAATGC TGGCAGCGCT GAGCTTACCA TTGAAATTAT CTCAGACAAT
    GGTATGCAAG CCGAAGTACA CATCCAGGAC AATGGGGATG GAACTTACAC CATTACATAC
    ATCCCACTGT ACCCAGGGGT CTACACTATC ACCATTAAGT ATGGAGGACA GATGGTGCCC
    AACTTCCCAA GTAAGCTGCA AGTTAAACCT GCCATTGACA CCTCTGGGGT TAAAGTCTAT
    GGACCAGGAG TTGAAGGGAA AGGGGTTTTC AGAGAAGCTA CTACAGATTT CAATGTGGAT
    GCTCGGGCTC TAACTAAATC TGGAGGGCCT CATCTCAAGA CCGTGGTCTC CAATCCTTCT
    GGCAACTTTA CTGAGGCATT TGTGCAAGAC AACGGAGATG GCACATACAA AGTGGAATAT
    ACTCCATATG AAGAGGGAAT CCACTCTGTT GATGTAATCT ACGACGGAAG CCCAGCCCCA
    AAAAGTCCCT TTGTGGTCCC TGTGACAGAA GGGTGTGATC CAAGCCGTGT CAGAGTCCAT
    GGCCCTGGCA TTGTGAGCGG TACCACAAAC CAACCAAACA AATTCACTGT GGAGACAAGA
    GGAGCTGGTA CAGGAGGGCT TGGCTTGGCT GTTGAAGGTC CATCAGAAGC AAAGATGTCC
    TGTACCGATA ACAAGGATGG CAGCTGTTCT GTGGAATACG TACCGTACGA ACCTGGCACA
    TACAACCTTA ATGTCACATA TGGTGGACAT CAAGTTCCAG GTAGCCCCTT TAAAGTTCCA
    GTTAGCGATG TGGTCGATAG CTCAAAAGTC AAATGTACCG GCCCTGGTTT GAGCCCCGGT
    CTTGTAAGAG CAAACGTCCC TCAGTCATTC AGTGTGGACA CAAGCAAAGC TGGAGTTGCT
    CCTCTGCAAG TCAAAGTTCA AGGTCCCAAG GGTATTGTTG AACCAGTGGA AATTGTGGAT
    AATGGAAATG GCGTCCAGAC TGTCAACTAT GTGCCAACTA GAGAAGGACC TTATAACATC
    TCTGTACTTT ATGGTGATGA TGAGATACCT CACAGCCCAT TTAAGGTTAG GGTTCTGCCA
    ACTCATGATG CAAGTAAGGT GAAGGCAAGC GGACCAGGCT TAAACACCAC TGGAGTTCCT
    GCCAGCTTGC CTGTGGAGTT CACTATTGAT GCCAAGGATG CTGGAGAGGG CCTGCTTGCT
    GTTCAGATAA CTGATCCAGA GGGCAAGCCC AAGAAGGCTA CAATCCGTGA TAATCAGGAT
    GGCACCTATA CGGTGTCCTA TGTGCCTGAC ATGACTGGTC GGTACACTAT CCTGATAAAG
    TATGGAGGCG ATGAGATCCC TTATTCTCCT TATCGCATCC GTGCGCTTCC CACTGGGGAC
    GCCAGCAAGT GCACAGTGAC AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29247
    Clone ID Related Accession (Same CDS sequence) XM_012968612.2 , XM_012968612.3
    Accession Version XM_012968612.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product filamin-A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACAGAG GTCTTTACGC TTGCAATCCC GCCGCCTGCC GTGCTGTGGG CCGTGGCTTG 
    CAGCCAAAGG GTGTACGCGT GAAGGAGACG GCGGATTTTA AGGTTTATAC TAAAGGCGCA
    GGAAGCGGTG AACTTAAAGT TGTGGTCAAG GGACCAAAGG GTACAGAGGA ACGTGTCAAG
    CAGAAGGACC TAGGAGATGG TGTGTATGCC TTTGAGTACT ATCCAGCTAC ACCTGGGAAC
    TATATTGTTA CCATCACATG GGGCGGACAG AATATCCCCA GAAGTCCATT TGAGGTAAAA
    GTTGGCACTG AATGTGGACA ACAAAGAGTT CGAGCATGGG GCCCTGGTCT TGAAGGAGGG
    GTGGTTGGAA AATCAGCTGA CTTTGTTGTA GAAGCAATCG GTGATGATGT TGGGACTCTC
    GGGTTTTCTG TGGAAGGTCC ATCTCAAGCT AAGATCGAGT GTGACGATAA AGGAGATGGG
    TCCTGTGATG TGCGCTACTG GCCACTGGAA CCTGGAGAGT ATGCGGTGCA TGTTCTGTGC
    AACAATGAGG ACATCAAACT GAGTCCTTTC ATGGCTGATA TCAAACCAGC CCCCAAAGAC
    TTCTACCCAG AGAAGGTCAA GGCCTATGGT CCTGGGCTAG AGAAGACTGG GTTGGCAATA
    AACAAACCAG CAGAGTTCAC TGTTGATGCC AAACTTGGAG GAAAGGCTCC TCTCAAGGTG
    TCTGTGCAGG ATTCTGAAGG CACTCCAGTG GATGTCACAA CCAAAGATAA TGGAAATGGC
    ACATACAGCT GCAGTTATTT GCCAAAGAAA CCTCTGAAAC ACACAGCTGT GGTGTCTTGG
    GGTGGGGTGA ACATTCCCAA CAGTCCTTTC AGGATGACTA TTGGTGCTGG AAGCCACCCT
    AACAAGGTGA AGGTTTATGG ACCTGGTGTT GCTAAGACTG GCCTGAAAGC TCATGAACCA
    ACCTACTTTA CTGTGGATTG CACAGAAGCT GGTCAAGGTG ATGTGAGTAT TGGAATTAAA
    TGTGCCCCAG GAGTTGTGGG CCCGGCTGAG GCGGACATAG ACTTTGACAT TATCCGCAAT
    GATAATGATA CATTCACCGT GAAGTACACT CCTCCTGGAG CTGGGAGCTA CACCATCATG
    GTCCTGTTTG CTGACCAGGC TACTCCAATG AGTCCATTCC GTATCAAGGT TGATCCATCA
    CATGATGCAA GTAAAGTTAA GGCAGAAGGA CCTGGACTAA ATAGAAGTGG TGTAGAAATT
    GGAAAACCAA CTCATTTCAC AGTTAACACC AAGGCTGCTG GAAAAGCCAA CCTAGACGTG
    CAGTTTACAG GTCCAGCCAA GGGAGAGGCT GTGAAGGACT TTGATGTTAT TGACAACCAC
    GATAACACCT ACACCGTAAA ATACACTCCA GTGCAACAGG GTAACATGGG TGTAAATGTG
    ACCTATGGTG GTGACCATAT TCCAAAGAGT CCCTTCACCG TTGGCATTGC CCCAACTTTG
    GACCTCAGTA AAATCAAAGT TTCTGGACTT GGTGACAAGG TGGAGGTCGG GAAGGATCAG
    GAGTTCACAG TAAAATCCAG GGGTGCTGGA GGACAAGGCA AAGTGGCTTG CAAGATCACA
    GGACCATCCA ACAAGTCTGT TCCTTGCAAG GTGGAACCTG GTCTGAGCCC AGATAACAGC
    ACTGTCAAAT TCATCCCTAG GGAGGAAGGC CCATATGAGG TAGACGTGAC GTACGACGGA
    GTGCCTATAC CTGGTAGCCC ATTTCCCGTT GAGGCAGTTC CTTCAACAAA TCCTTCAAAG
    GTCAAAGCTT TTGGACCTGG TCTAAAAGGA GGCAGTGTTG GTTCCTCTGC ACCCTTTACT
    ATTGACACTA AAGGTGCAGG CCAAGGTGGG CTTGGTCTCA CTGTTGAAGG TCCATGTGAA
    GCCAAAATAG AATGTCTAGA CAATGGAGAT GGAACCTGTT CTGTCTCTTA CTTGCCTACA
    GAACGTGGTG ACTATAATAT CAATATCTTG TTTGCTGATA CACATATCCC TGGATCACCT
    TTCAAGGCTA AGGTTGAGCC ATGTTTCGAT CCTTCAAAGG TGACCTGTTC TGGTCCTGGT
    CTGGAACATG CTCAGGTTGG AGAAGCTGGT AAATTTAATG TGGACTGTTC AAATGCTGGC
    AGCGCTGAGC TTACCATTGA AATTATCTCA GACAATGGTA TGCAAGCCGA AGTACACATC
    CAGGACAATG GGGATGGAAC TTACACCATT ACATACATCC CACTGTACCC AGGGGTCTAC
    ACTATCACCA TTAAGTATGG AGGACAGATG GTGCCCAACT TCCCAAGTAA GCTGCAAGTT
    AAACCTGCCA TTGACACCTC TGGGGTTAAA GTCTATGGAC CAGGAGTTGA AGGGAAAGGG
    GTTTTCAGAG AAGCTACTAC AGATTTCAAT GTGGATGCTC GGGCTCTAAC TAAATCTGGA
    GGGCCTCATC TCAAGACCGT GGTCTCCAAT CCTTCTGGCA ACTTTACTGA GGCATTTGTG
    CAAGACAACG GAGATGGCAC ATACAAAGTG GAATATACTC CATATGAAGA GGGAATCCAC
    TCTGTTGATG TAATCTACGA CGGAAGCCCA GCCCCAAAAA GTCCCTTTGT GGTCCCTGTG
    ACAGAAGGGT GTGATCCAAG CCGTGTCAGA GTCCATGGCC CTGGCATTGT GAGCGGTACC
    ACAAACCAAC CAAACAAATT CACTGTGGAG ACAAGAGGAG CTGGTACAGG AGGGCTTGGC
    TTGGCTGTTG AAGGTCCATC AGAAGCAAAG ATGTCCTGTA CCGATAACAA GGATGGCAGC
    TGTTCTGTGG AATACGTACC GTACGAACCT GGCACATACA ACCTTAATGT CACATATGGT
    GGACATCAAG TTCCAGGTAG CCCCTTTAAA GTTCCAGTTA GCGATGTGGT CGATAGCTCA
    AAAGTCAAAT GTACCGGCCC TGGTTTGAGC CCCGGTCTTG TAAGAGCAAA CGTCCCTCAG
    TCATTCAGTG TGGACACAAG CAAAGCTGGA GTTGCTCCTC TGCAAGTCAA AGTTCAAGGT
    CCCAAGGGTA TTGTTGAACC AGTGGAAATT GTGGATAATG GAAATGGCGT CCAGACTGTC
    AACTATGTGC CAACTAGAGA AGGACCTTAT AACATCTCTG TACTTTATGG TGATGATGAG
    ATACCTCACA GCCCATTTAA GGTTAGGGTT CTGCCAACTC ATGATGCAAG TAAGGTGAAG
    GCAAGCGGAC CAGGCTTAAA CACCACTGGA GTTCCTGCCA GCTTGCCTGT GGAGTTCACT
    ATTGATGCCA AGGATGCTGG AGAGGGCCTG CTTGCTGTTC AGATAACTGA TCCAGAGGGC
    AAGCCCAAGA AGGCTACAAT CCGTGATAAT CAGGATGGCA CCTATACGGT GTCCTATGTG
    CCTGACATGA CTGGTCGGTA CACTATCCTG ATAAAGTATG GAGGCGATGA GATCCCTTAT
    TCTCCTTATC GCATCCGTGC GCTTCCCACT GGGGACGCCA GCAAGTGCAC AGTGACAGTG
    TCAATCGGAG GTCACGGGCT AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824066.1
    CDS50..6580
    Translation

    Target ORF information:

    RefSeq Version XM_012968612.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis filamin A (flna), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968612.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGACAGAG GTCTTTACGC TTGCAATCCC GCCGCCTGCC GTGCTGTGGG CCGTGGCTTG 
    CAGCCAAAGG GTGTACGCGT GAAGGAGACG GCGGATTTTA AGGTTTATAC TAAAGGCGCA
    GGAAGCGGTG AACTTAAAGT TGTGGTCAAG GGACCAAAGG GTACAGAGGA ACGTGTCAAG
    CAGAAGGACC TAGGAGATGG TGTGTATGCC TTTGAGTACT ATCCAGCTAC ACCTGGGAAC
    TATATTGTTA CCATCACATG GGGCGGACAG AATATCCCCA GAAGTCCATT TGAGGTAAAA
    GTTGGCACTG AATGTGGACA ACAAAGAGTT CGAGCATGGG GCCCTGGTCT TGAAGGAGGG
    GTGGTTGGAA AATCAGCTGA CTTTGTTGTA GAAGCAATCG GTGATGATGT TGGGACTCTC
    GGGTTTTCTG TGGAAGGTCC ATCTCAAGCT AAGATCGAGT GTGACGATAA AGGAGATGGG
    TCCTGTGATG TGCGCTACTG GCCACTGGAA CCTGGAGAGT ATGCGGTGCA TGTTCTGTGC
    AACAATGAGG ACATCAAACT GAGTCCTTTC ATGGCTGATA TCAAACCAGC CCCCAAAGAC
    TTCTACCCAG AGAAGGTCAA GGCCTATGGT CCTGGGCTAG AGAAGACTGG GTTGGCAATA
    AACAAACCAG CAGAGTTCAC TGTTGATGCC AAACTTGGAG GAAAGGCTCC TCTCAAGGTG
    TCTGTGCAGG ATTCTGAAGG CACTCCAGTG GATGTCACAA CCAAAGATAA TGGAAATGGC
    ACATACAGCT GCAGTTATTT GCCAAAGAAA CCTCTGAAAC ACACAGCTGT GGTGTCTTGG
    GGTGGGGTGA ACATTCCCAA CAGTCCTTTC AGGATGACTA TTGGTGCTGG AAGCCACCCT
    AACAAGGTGA AGGTTTATGG ACCTGGTGTT GCTAAGACTG GCCTGAAAGC TCATGAACCA
    ACCTACTTTA CTGTGGATTG CACAGAAGCT GGTCAAGGTG ATGTGAGTAT TGGAATTAAA
    TGTGCCCCAG GAGTTGTGGG CCCGGCTGAG GCGGACATAG ACTTTGACAT TATCCGCAAT
    GATAATGATA CATTCACCGT GAAGTACACT CCTCCTGGAG CTGGGAGCTA CACCATCATG
    GTCCTGTTTG CTGACCAGGC TACTCCAATG AGTCCATTCC GTATCAAGGT TGATCCATCA
    CATGATGCAA GTAAAGTTAA GGCAGAAGGA CCTGGACTAA ATAGAAGTGG TGTAGAAATT
    GGAAAACCAA CTCATTTCAC AGTTAACACC AAGGCTGCTG GAAAAGCCAA CCTAGACGTG
    CAGTTTACAG GTCCAGCCAA GGGAGAGGCT GTGAAGGACT TTGATGTTAT TGACAACCAC
    GATAACACCT ACACCGTAAA ATACACTCCA GTGCAACAGG GTAACATGGG TGTAAATGTG
    ACCTATGGTG GTGACCATAT TCCAAAGAGT CCCTTCACCG TTGGCATTGC CCCAACTTTG
    GACCTCAGTA AAATCAAAGT TTCTGGACTT GGTGACAAGG TGGAGGTCGG GAAGGATCAG
    GAGTTCACAG TAAAATCCAG GGGTGCTGGA GGACAAGGCA AAGTGGCTTG CAAGATCACA
    GGACCATCCA ACAAGTCTGT TCCTTGCAAG GTGGAACCTG GTCTGAGCCC AGATAACAGC
    ACTGTCAAAT TCATCCCTAG GGAGGAAGGC CCATATGAGG TAGACGTGAC GTACGACGGA
    GTGCCTATAC CTGGTAGCCC ATTTCCCGTT GAGGCAGTTC CTTCAACAAA TCCTTCAAAG
    GTCAAAGCTT TTGGACCTGG TCTAAAAGGA GGCAGTGTTG GTTCCTCTGC ACCCTTTACT
    ATTGACACTA AAGGTGCAGG CCAAGGTGGG CTTGGTCTCA CTGTTGAAGG TCCATGTGAA
    GCCAAAATAG AATGTCTAGA CAATGGAGAT GGAACCTGTT CTGTCTCTTA CTTGCCTACA
    GAACGTGGTG ACTATAATAT CAATATCTTG TTTGCTGATA CACATATCCC TGGATCACCT
    TTCAAGGCTA AGGTTGAGCC ATGTTTCGAT CCTTCAAAGG TGACCTGTTC TGGTCCTGGT
    CTGGAACATG CTCAGGTTGG AGAAGCTGGT AAATTTAATG TGGACTGTTC AAATGCTGGC
    AGCGCTGAGC TTACCATTGA AATTATCTCA GACAATGGTA TGCAAGCCGA AGTACACATC
    CAGGACAATG GGGATGGAAC TTACACCATT ACATACATCC CACTGTACCC AGGGGTCTAC
    ACTATCACCA TTAAGTATGG AGGACAGATG GTGCCCAACT TCCCAAGTAA GCTGCAAGTT
    AAACCTGCCA TTGACACCTC TGGGGTTAAA GTCTATGGAC CAGGAGTTGA AGGGAAAGGG
    GTTTTCAGAG AAGCTACTAC AGATTTCAAT GTGGATGCTC GGGCTCTAAC TAAATCTGGA
    GGGCCTCATC TCAAGACCGT GGTCTCCAAT CCTTCTGGCA ACTTTACTGA GGCATTTGTG
    CAAGACAACG GAGATGGCAC ATACAAAGTG GAATATACTC CATATGAAGA GGGAATCCAC
    TCTGTTGATG TAATCTACGA CGGAAGCCCA GCCCCAAAAA GTCCCTTTGT GGTCCCTGTG
    ACAGAAGGGT GTGATCCAAG CCGTGTCAGA GTCCATGGCC CTGGCATTGT GAGCGGTACC
    ACAAACCAAC CAAACAAATT CACTGTGGAG ACAAGAGGAG CTGGTACAGG AGGGCTTGGC
    TTGGCTGTTG AAGGTCCATC AGAAGCAAAG ATGTCCTGTA CCGATAACAA GGATGGCAGC
    TGTTCTGTGG AATACGTACC GTACGAACCT GGCACATACA ACCTTAATGT CACATATGGT
    GGACATCAAG TTCCAGGTAG CCCCTTTAAA GTTCCAGTTA GCGATGTGGT CGATAGCTCA
    AAAGTCAAAT GTACCGGCCC TGGTTTGAGC CCCGGTCTTG TAAGAGCAAA CGTCCCTCAG
    TCATTCAGTG TGGACACAAG CAAAGCTGGA GTTGCTCCTC TGCAAGTCAA AGTTCAAGGT
    CCCAAGGGTA TTGTTGAACC AGTGGAAATT GTGGATAATG GAAATGGCGT CCAGACTGTC
    AACTATGTGC CAACTAGAGA AGGACCTTAT AACATCTCTG TACTTTATGG TGATGATGAG
    ATACCTCACA GCCCATTTAA GGTTAGGGTT CTGCCAACTC ATGATGCAAG TAAGGTGAAG
    GCAAGCGGAC CAGGCTTAAA CACCACTGGA GTTCCTGCCA GCTTGCCTGT GGAGTTCACT
    ATTGATGCCA AGGATGCTGG AGAGGGCCTG CTTGCTGTTC AGATAACTGA TCCAGAGGGC
    AAGCCCAAGA AGGCTACAAT CCGTGATAAT CAGGATGGCA CCTATACGGT GTCCTATGTG
    CCTGACATGA CTGGTCGGTA CACTATCCTG ATAAAGTATG GAGGCGATGA GATCCCTTAT
    TCTCCTTATC GCATCCGTGC GCTTCCCACT GGGGACGCCA GCAAGTGCAC AGTGACAGTG
    TCAATCGGAG GTCACGGGCT AGGTGCCGGC ATTGGTCCAA CTATACAGAT TGGCGAAGAA
    ACCGTAATAA CAGTTGATGC CAAAGCAGCT GGGAAGGGTA AAGTGACCTG CACTGTGTGC
    ACTCCAGATG GCACAGAAGT AGATGTTGAT GTGGTAGAGA ATGAAGATGG CACCTTTGAC
    ATATTCTATA CAGCTCCACA ACCTGGAAAA TATGTTATTT GTGTGCGCTT CGGTGGGGAA
    CACATTCCCA ACAGTCCTTT CCAGGTCACG GCTCTGGACG GCGAGCAAAT GATGCCTCAG
    CAGATGGTTC AACAGCAGTT AGCCCCACCA TACGCTTACC CGGGCATGCA ACAACCCTGG
    GCTACTGATC GTCCAGTTAT GGGTGTAAAT GGACTGGATG TGGCAGGACT TAGACCTTTT
    GATCTGGTTA TTCCCTTCAC CATTAAAAAA GGAGAGATTA CAGGAGAAGT GCGAATGCCA
    TCAGGGAAGG TGGCCAAGCC TGACATCACT GATAACAAAG ATGGAACAGT CACGGTGAGA
    TATGCTCCCA CTGAAGCTGG ACTCCATGAG ATGGATATAA AGTATGATGG CATGCATATT
    CCAGGAAGCC CCCTACAGTT TTATGTGGAT TATGTAAACA GTGGCCATGT TACTGCCTAT
    GGCCCAGGCT TGATTCATGG AACAGTGAAT AAACCTGCTG TCTTCACTGT GAACACTAAG
    GATGCTGGAG AAGGTGGCTT GTCACTAGCT ATTGAGGGCC CCTCCAAAGC AGAGATCAGC
    TGCACAGATA ACCAGGATGG GACCTGCACA GTTTCTTATC TCCCTGTGCT CCCTGGAGAC
    TACAACATCC TAGTCAAGTA TAACAACAAG CATATTCCGG GAAGTCCATT TGTTGCTAAA
    ATAACAGGAG ATGATTCTAT GCGTATGTCC CAGCTGAAGG TTGGGTCTGC AGCAGACATC
    CCCCTGAACA TTGTAGAGAC AGACCTGAGC CAGCTGACTG CTACAGTGAC CTCTCCATCT
    GGAAGAGAGG AGCCATGTCT GCTCAAGAGG CTCAGAAACG GCCATGTTGG CATCTCGTTT
    GTGCCAAAGG AGACAGGCGA ACACATTGTT AGCATAAAAA AAAATGGTAT CCACATCCCC
    AACAGCCCAA TCACTGTAAT GATCAGCCAA TCCGAAATCG GGGATGCCAG CCGAGTGATA
    GTGTCTGGTC AAGGTCTGAC TGAAGGGAGG ACATTTGAGC CCGCAGAATT CATCATTGAC
    ACCAGGGAAG CAGGTTATGG TGGACTTAGC CTTTCCATCG AGGGGCCCAG CAAAGTGGAT
    ATCAACACAG AAGACTTGGA AGATGGGACA TGCAAAGTTA CTTACTGCCC AACTGAACCT
    GGAAATTATA TTATCAACAT AAAGTTTGCT GATCAGCATG TTCCTGGCAG CCCCTTCTCT
    GTCAAGGTGA CTGGGGAAGG AAGAATGAAG GAGAGTATCA CTCGAAGACG CACCGCTCCA
    TCTGTGGCAA ACATTGGTAG CCAGTGTGAT CTGAGCCTAA AAATACCCGA GATCAGCATT
    CATGACATGA CTGCCCAGGT CACTAGTCCC TCAGGGAAGA TTCATGATGC AGAAATAATG
    GAAAGCGAGA ATAACACTTA CTGCATCCGA TTTGTTCCTT CTGAGATGGG CGTCCACACA
    GTCAGTGTTA AGTACAAGGG ACAGCATGTG CCTGGAAGCC CCTTTCAGTT CACTGTTGGC
    CCCTTGGGTG AAGGAGGAGC CCACAAAGTA CGAGCTGGGG GTCCTGGCCT GGAAAGAGCG
    GAGGTTGGAA CACCAGCGGA GTTCAGTATC TGGACCAGAG AGGCAGGAGC TGGTGGTCTA
    TCAATTGCAG TGGAAGGTCC AAGCAAAGCA GAGATTGCAT TTGAGGACAG AAAGGATGGT
    TCTTGTGGAG TGTCCTACAT TGTGCAGGAG CCTGGTGATT ATGAGGTGTC TGTGAAGTTT
    AATGATGAGC ACATACCAGA CAGCCCCTTC GTGGTTCCAG TGGGTTCGAC ATCAGATGAT
    GCACGGAGAC TTACTGTTTC TAGTCTTCAG GAGTCAGGGT TAAAGGTCAA CCAGCCAGCC
    TCTTTTGCAG TAAGTCTAAA TGGTGCCAAA GGAGTTCTAG ATGCCAAGGT CCACAGCCCC
    TCAGGTGCCT TGGAGGAATG CTGTGTGACA GAAATTGATG AAGATAAATA TGCTGTGCGC
    TTTATTCCCC GTGAAAATGG TCTATATATT GTCGATGTAA AGTTTAACGG CTCCCATATA
    CCTGGCAGTC CCTTCAAAAT AAGAGTAGGG GAGCCAGGCC AAGCTGGGGA TCCAGGAATG
    GTATCTGCAT ATGGCCCTGG GTTAGAAGGA GGCACCACAG GCAGTGCTGC AGAGTTCATT
    GTCAACACCA CAAAAGCAGG CCCTGGGGCA CTCACAGTCA CAATTGATGG CCCGTCCAAA
    GTCAAAATGG ACTGCCAGGA ATGTGCCGAG GGCTATAAGG TCACCTACAC TCCTATGGCT
    CCTGGAAGCT ACCTGATATC CATCAAGTAC GGGGGACCCT ACCATATTGT GGGAAGCCCA
    TTCAAAGCAA AGGTTACAGG TCCTCGCCTA GTGACCAGCC ACAGCCTACA TGAATCCTCT
    TCAGTCTTTG TTGATTCTGT CACCAAATCT GAGGCCTTCA CTCAGCATGC CGCAGCTCCT
    AGGATAGCAT CAGACGCCAG CAAAGTAGTG GCCAAAGGGC TAGGACTCAA CAAAGCCTTT
    GTAGGCCAGA AGAACTCTTT CACTGTGGAC TGCAGTAAAG CTGGTAACAA CATGCTGCTT
    GTCGGAGTTC ATGGACCGAA AACCCCATGT GAAGAGATAG TGGTGAAGCA CCTTGGGAAC
    CGTCTCTACA ACGTTACGTA CCTCCTGAAG GATAAGGGGG ATTACATCCT TGTAGTAAAA
    TGGGGCGATG AGCACATTCT GGGAAGCCCT TACCATGTGT CTGTGCCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.