slc12a5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a5
Entrez Gene ID	779759
Full Name	solute carrier family 12 member 5
Synonyms	kcc2
General protein information	Preferred Names solute carrier family 12 member 5 Names solute carrier family 12 member 5 solute carrier family 12 (potassium/chloride transporter), member 5 solute carrier family 12, (potassium-chloride transporter) member 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012952549.3	XP_012808003.1	solute carrier family 12 member 5 isoform X1
XM_012952550.1	XP_012808004.1	solute carrier family 12 member 5 isoform X2
XM_012952549.2	XP_012808003.1	solute carrier family 12 member 5 isoform X1
XM_012952550.2	XP_012808004.1	solute carrier family 12 member 5 isoform X2
XM_031894159.1	XP_031750019.1	solute carrier family 12 member 5 isoform X3
NM_001078838.2	NP_001072306.2	solute carrier family 12 member 5

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC12A5	XP_001236722.3
	SLC12A5	XP_001104798.1
Bos taurus (cattle)	SLC12A5	NP_001193309.1
Pan troglodytes (chimpanzee)	SLC12A5	XP_003317037.1
Rattus norvegicus (Norway rat)	Slc12a5	NP_599190.1
Danio rerio (zebrafish)	slc12a5b	XP_003199160.2
Danio rerio (zebrafish)	LOC572215	XP_701000.5
Oryza sativa (rice)	Os08g0323700	NP_001061543.1
Homo sapiens (human)	SLC12A5	NP_065759.1
Canis lupus familiaris (dog)	SLC12A5	XP_005635409.1
Mus musculus (house mouse)	Slc12a5	NP_065066.2
Arabidopsis thaliana (thale cress)	CCC1	NP_174333.2
Xenopus tropicalis (tropical clawed frog)	slc12a5	NP_001072306.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc12a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa50175	XM_031894159.1 Latest version!	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa29158	XM_012952549.3 Latest version!	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29159	XM_012952550.2 Latest version!	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa24407	NM_001078838.2 Latest version!	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa29158	XM_012952549.2	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29159	XM_012952550.1	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a5 ( XM_031894159.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750019.1 Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa50175
Clone ID Related Accession (Same CDS sequence)	XM_031894159.1
Accession Version	XM_031894159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACTG CTATCTCAAT GAGCGCCATC GCTACCAATG GAGTTGTGCC AGCTGGTGGA 
TCATATTACA TGATCTCTCG ATCGCTTGGG CCGGAGTTTG GAGGAGCTGT GGGACTTTGC 
TTTTATCTTG GAACCACATT TGCAGGTGCC ATGTACATCC TGGGAACCAT AGAGATCCTG 
CTGACTTATC TCTTCCCTGG CATGGCTATC TTTATCGCTC AGGAAGCTAG GGAGGAACCT 
GCAGCATTGC TCAACAATAT GCGTGTTTAT GGCACATGTG TGCTCACCTG CATGGCCATT 
GTAGTTTTTG TAGGAGTTAA ATATGTGAAT AAGTTTGCCC TGGTTTTCCT GGGCTGTGTC 
ATCTTGTCCA TCATCGCAAT ATACGCCGGG GTAATCAAGT CCGCTTTCGA CCCTCCAGAT 
TTTCCTATCT GCCTTCTTGG TAATCGTACA CTCTCCAGGA AAGGGTTTGA TGTCTGTGCC 
AAGTTTATTG ACAGTGGCAA CGAGACAGTC ACCACCAAGC TGTGGGCTCT CTTCTGCTCC 
TCACCATTCC TGAATGCCAC ATGTGATACA TACTTCTCTC AGAACAACGT GACTGAGGTT 
CAGGGCATCC CTGGTGTAGC CAGTGGTCTT ATTCGAGATA ACCTTTGGGC TGGTTATATG 
CAGAAAGGTA TCATAGTGGA GAAACAAGGA CTACGATCTG CTCCTAATTC AGATGCCCTC 
AATCTGGATC AGCCTTATGT TTTCAGTGAT ATGACCACCT ACTTTACTCT TCTGGTGGGG 
ATCTACTTCC CCTCAGTTAC AGGCATTATG GCTGGTTCCA ACAGGTCTGG AGATCTTCGG 
GATGCCCAGA AATCCATCCC CATTGGCACT ATTATGGCCA TAGCCACCAC ATCATTTGTG 
TATATCTCTT CAGTTATTTT ATTTGGAGCA TGCATCGAGG GGGTTGTCTT GCGTGACAAA 
TTTGGAGAGG CGGTGAATGG AAATTTAGTG GTAGGCACTC TAGCATGGCC ATCCCCTTGG 
GTCATTGTGA TTGGATCTTT TTTCTCTACA TGTGGGGCAG GTTTGCAGAG TTTAACAGGG 
GCCCCACGTC TTCTACAAGC AATTGCTCGA GATGGAATTG TTCCGTTTCT CCAGGTCTTT 
GGTCATGGGA AAGCTAATGG AGAGCCAACA TGGGCCCTTC TCCTCACCGC CTGTATCTGT 
GAGATTGGTA TCCTCATTGC ATCCCTGGAT GAAGTGGCTC CAATTCTTTC AATGTTTTTC 
TTAATGTGTT ACATGTTTGT TAACTTGGCC TGTGCAGTGC AGACCTTGTT AAGGACTCCC 
AACTGGAGGC CTCGTTTCAA ATATTATCAC TGGACTCTTT CATTTCTTGG GATGAGTCTT 
TGTCTGTCTC TTATGTTTAT CTGCTCCTGG TACTATGCTC TTGTTGCCAT GTCGATCGCT 
GGACTTATTT ATAAATACAT TGAGTACAGA GGGGCAGAGA AAGAGTGGGG TGATGGGATC 
CGCGGTCTGT CCCTCAGTGC TGCTCGATAT GCACTACTGC GGTTAGAAGA AGGGCCACCA 
CACACGAAGA ACTGGAGGCC TCAGTTGCTG GTTCTAGTAC GTGTGGACCA GGATCAAAAT 
GTTGTTCACC CGCAGCTCCT TTCATTAACC TCTCAACTGA AGGCAGGAAA GGGTCTGACG 
ATTGTTGGCT CAGTGGTAGA GGGAACATAC TTAGAAAACC ACCCTCAGTG TCAGCGTGCT 
GAAGAGTCTA TTCGCCGACT CATGGAGGCA GAGAAAGTTA AAGGTTTCTG CAAGGTGGTT 
ATTTCATCAA ATACCCGGGA TGGGATTTCC CATCTGATTC AGTCTAGTGG ACTTGGAGGC 
TTGCAGCACA ATACCCTGCT AGTGGGTTGG CCACGAAACT GGAGGCAAAA AGAAGACCAC 
CAAACCTGGA GAAATTTCAT TGAACTTGTA AGAGAAACAA CTGCTGCTCA CCTGGCTCTA 
ATTGTTAGCA AGAATGTAGC TATGTTCCCT GGAAACCTAG AGAGATTTTC TGAAGGCAAC 
ATTGATGTTT GGTGGGTGGT CCATGATGGA GGAATGTTGA TGTTACTGCC ATTCCTCCTG 
CGTCATCACA AGGTTTGGAA GAGATGTACA ATGCGCATAT TTACTGTTGC ACAGATGGAC 
GACAACAGCA TACAGATGAA GAAAGACTTG ACCACTTTCC TTTATCATTT AAGAATTAAT 
GCTGAGGTAG AGGTGGTTGA AATGCATGAC AGCGACATCT CGGCCTATAC TTATGAGAAG 
ACGCTAGTTA TGGAGCAGAG GTCCCAAATC CTAAAGCAGA TGCATCTCAC CAAAACTGAA 
CGTGAACGTG AGATTCAAAG TATTACAGAT GAATCCCGTG GATCCATCAG ACGTAAGAAT 
CCTTCCAACT CCAGGCTGCA CTTGAGCGTT CCAGAAGAGC AGGTGGGAGA CTGTGAGGAG 
AAGTCCGAAG AAGAGGTACA GCTTATTCAT GACAAAAATA CAACAGGCTT TTCAGAGAGT 
GCCCAGTCTC CAGCAGAAGA ACCAGACACA ACTCAAGAGA AAGTGCACCT TACCTGGACT 
AAAGACAAAT GCACAGCAGA AAAAAACAGT AGTAAATGTC CCAGCACTCC AGAGGCCATT 
AAAGATTTCT TCAACATGAA GCCTGAATGG GAAAACTTAA ACCAGTCAAA TGTACGAAGA 
ATGCACACAG CTGTGAAACT CAATGAAGTA ATTGTGAAAA AATCCCATGA TGCAAAACTG 
GTTCTACTAA ATATGCCTGG ACCACCCCGC AACAGGAAAG GGGACGAAAA TTATATGGAG 
TTTTTAGAAG TCCTCACAGA GCACCTGGAT CGGGTTATGT TGGTTCGTGG TGGAGGACGC 
GAGGTTATTA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750019.1
CDS	8..2911
Translation	MLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPEWENLNQSNVRRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_031894159.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894159.1

ORF Insert Sequence:

ATGCTGACTG CTATCTCAAT GAGCGCCATC GCTACCAATG GAGTTGTGCC AGCTGGTGGA 
TCATATTACA TGATCTCTCG ATCGCTTGGG CCGGAGTTTG GAGGAGCTGT GGGACTTTGC 
TTTTATCTTG GAACCACATT TGCAGGTGCC ATGTACATCC TGGGAACCAT AGAGATCCTG 
CTGACTTATC TCTTCCCTGG CATGGCTATC TTTATCGCTC AGGAAGCTAG GGAGGAACCT 
GCAGCATTGC TCAACAATAT GCGTGTTTAT GGCACATGTG TGCTCACCTG CATGGCCATT 
GTAGTTTTTG TAGGAGTTAA ATATGTGAAT AAGTTTGCCC TGGTTTTCCT GGGCTGTGTC 
ATCTTGTCCA TCATCGCAAT ATACGCCGGG GTAATCAAGT CCGCTTTCGA CCCTCCAGAT 
TTTCCTATCT GCCTTCTTGG TAATCGTACA CTCTCCAGGA AAGGGTTTGA TGTCTGTGCC 
AAGTTTATTG ACAGTGGCAA CGAGACAGTC ACCACCAAGC TGTGGGCTCT CTTCTGCTCC 
TCACCATTCC TGAATGCCAC ATGTGATACA TACTTCTCTC AGAACAACGT GACTGAGGTT 
CAGGGCATCC CTGGTGTAGC CAGTGGTCTT ATTCGAGATA ACCTTTGGGC TGGTTATATG 
CAGAAAGGTA TCATAGTGGA GAAACAAGGA CTACGATCTG CTCCTAATTC AGATGCCCTC 
AATCTGGATC AGCCTTATGT TTTCAGTGAT ATGACCACCT ACTTTACTCT TCTGGTGGGG 
ATCTACTTCC CCTCAGTTAC AGGCATTATG GCTGGTTCCA ACAGGTCTGG AGATCTTCGG 
GATGCCCAGA AATCCATCCC CATTGGCACT ATTATGGCCA TAGCCACCAC ATCATTTGTG 
TATATCTCTT CAGTTATTTT ATTTGGAGCA TGCATCGAGG GGGTTGTCTT GCGTGACAAA 
TTTGGAGAGG CGGTGAATGG AAATTTAGTG GTAGGCACTC TAGCATGGCC ATCCCCTTGG 
GTCATTGTGA TTGGATCTTT TTTCTCTACA TGTGGGGCAG GTTTGCAGAG TTTAACAGGG 
GCCCCACGTC TTCTACAAGC AATTGCTCGA GATGGAATTG TTCCGTTTCT CCAGGTCTTT 
GGTCATGGGA AAGCTAATGG AGAGCCAACA TGGGCCCTTC TCCTCACCGC CTGTATCTGT 
GAGATTGGTA TCCTCATTGC ATCCCTGGAT GAAGTGGCTC CAATTCTTTC AATGTTTTTC 
TTAATGTGTT ACATGTTTGT TAACTTGGCC TGTGCAGTGC AGACCTTGTT AAGGACTCCC 
AACTGGAGGC CTCGTTTCAA ATATTATCAC TGGACTCTTT CATTTCTTGG GATGAGTCTT 
TGTCTGTCTC TTATGTTTAT CTGCTCCTGG TACTATGCTC TTGTTGCCAT GTCGATCGCT 
GGACTTATTT ATAAATACAT TGAGTACAGA GGGGCAGAGA AAGAGTGGGG TGATGGGATC 
CGCGGTCTGT CCCTCAGTGC TGCTCGATAT GCACTACTGC GGTTAGAAGA AGGGCCACCA 
CACACGAAGA ACTGGAGGCC TCAGTTGCTG GTTCTAGTAC GTGTGGACCA GGATCAAAAT 
GTTGTTCACC CGCAGCTCCT TTCATTAACC TCTCAACTGA AGGCAGGAAA GGGTCTGACG 
ATTGTTGGCT CAGTGGTAGA GGGAACATAC TTAGAAAACC ACCCTCAGTG TCAGCGTGCT 
GAAGAGTCTA TTCGCCGACT CATGGAGGCA GAGAAAGTTA AAGGTTTCTG CAAGGTGGTT 
ATTTCATCAA ATACCCGGGA TGGGATTTCC CATCTGATTC AGTCTAGTGG ACTTGGAGGC 
TTGCAGCACA ATACCCTGCT AGTGGGTTGG CCACGAAACT GGAGGCAAAA AGAAGACCAC 
CAAACCTGGA GAAATTTCAT TGAACTTGTA AGAGAAACAA CTGCTGCTCA CCTGGCTCTA 
ATTGTTAGCA AGAATGTAGC TATGTTCCCT GGAAACCTAG AGAGATTTTC TGAAGGCAAC 
ATTGATGTTT GGTGGGTGGT CCATGATGGA GGAATGTTGA TGTTACTGCC ATTCCTCCTG 
CGTCATCACA AGGTTTGGAA GAGATGTACA ATGCGCATAT TTACTGTTGC ACAGATGGAC 
GACAACAGCA TACAGATGAA GAAAGACTTG ACCACTTTCC TTTATCATTT AAGAATTAAT 
GCTGAGGTAG AGGTGGTTGA AATGCATGAC AGCGACATCT CGGCCTATAC TTATGAGAAG 
ACGCTAGTTA TGGAGCAGAG GTCCCAAATC CTAAAGCAGA TGCATCTCAC CAAAACTGAA 
CGTGAACGTG AGATTCAAAG TATTACAGAT GAATCCCGTG GATCCATCAG ACGTAAGAAT 
CCTTCCAACT CCAGGCTGCA CTTGAGCGTT CCAGAAGAGC AGGTGGGAGA CTGTGAGGAG 
AAGTCCGAAG AAGAGGTACA GCTTATTCAT GACAAAAATA CAACAGGCTT TTCAGAGAGT 
GCCCAGTCTC CAGCAGAAGA ACCAGACACA ACTCAAGAGA AAGTGCACCT TACCTGGACT 
AAAGACAAAT GCACAGCAGA AAAAAACAGT AGTAAATGTC CCAGCACTCC AGAGGCCATT 
AAAGATTTCT TCAACATGAA GCCTGAATGG GAAAACTTAA ACCAGTCAAA TGTACGAAGA 
ATGCACACAG CTGTGAAACT CAATGAAGTA ATTGTGAAAA AATCCCATGA TGCAAAACTG 
GTTCTACTAA ATATGCCTGG ACCACCCCGC AACAGGAAAG GGGACGAAAA TTATATGGAG 
TTTTTAGAAG TCCTCACAGA GCACCTGGAT CGGGTTATGT TGGTTCGTGG TGGAGGACGC 
GAGGTTATTA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a5 ( XM_012952549.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808003.1 Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29158
Clone ID Related Accession (Same CDS sequence)	XM_012952549.3 , XM_012952549.2
Accession Version	XM_012952549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCTGA ATGGGAAAAC TTAAACCAGT CAAATGTACG AAGAATGCAC 
ACAGCTGTGA AACTCAATGA AGTAATTGTG AAAAAATCCC ATGATGCAAA ACTGGTTCTA 
CTAAATATGC CTGGACCACC CCGCAACAGG AAAGGGGACG AAAATTATAT GGAGTTTTTA 
GAAGTCCTCA CAGAGCACCT GGATCGGGTT ATGTTGGTTC GTGGTGGAGG ACGCGAGGTT 
ATTACCATCT ACTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808003.1
CDS	218..3535
Translation	MLNNLTECEEGEGGPSQGDGNPKESSPFINSSEMDTGKNMALFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPEWENLNQSNVRRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_012952549.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952549.3

ORF Insert Sequence:

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCTGA ATGGGAAAAC TTAAACCAGT CAAATGTACG AAGAATGCAC 
ACAGCTGTGA AACTCAATGA AGTAATTGTG AAAAAATCCC ATGATGCAAA ACTGGTTCTA 
CTAAATATGC CTGGACCACC CCGCAACAGG AAAGGGGACG AAAATTATAT GGAGTTTTTA 
GAAGTCCTCA CAGAGCACCT GGATCGGGTT ATGTTGGTTC GTGGTGGAGG ACGCGAGGTT 
ATTACCATCT ACTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a5 ( XM_012952550.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808004.1 Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X2, mRNA.

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CloneID	OXa29159
Clone ID Related Accession (Same CDS sequence)	XM_012952550.1 , XM_012952550.2
Accession Version	XM_012952550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCAAA CCAGTCAAAT GTACGAAGAA TGCACACAGC TGTGAAACTC 
AATGAAGTAA TTGTGAAAAA ATCCCATGAT GCAAAACTGG TTCTACTAAA TATGCCTGGA 
CCACCCCGCA ACAGGAAAGG GGACGAAAAT TATATGGAGT TTTTAGAAGT CCTCACAGAG 
CACCTGGATC GGGTTATGTT GGTTCGTGGT GGAGGACGCG AGGTTATTAC CATCTACTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808004.1
CDS	219..3521
Translation	MLNNLTECEEGEGGPSQGDGNPKESSPFINSSEMDTGKNMALFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPNQSNVRRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_012952550.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952550.2

ORF Insert Sequence:

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCAAA CCAGTCAAAT GTACGAAGAA TGCACACAGC TGTGAAACTC 
AATGAAGTAA TTGTGAAAAA ATCCCATGAT GCAAAACTGG TTCTACTAAA TATGCCTGGA 
CCACCCCGCA ACAGGAAAGG GGACGAAAAT TATATGGAGT TTTTAGAAGT CCTCACAGAG 
CACCTGGATC GGGTTATGTT GGTTCGTGGT GGAGGACGCG AGGTTATTAC CATCTACTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a5 ( NM_001078838.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072306.2 Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), mRNA.

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CloneID	OXa24407
Clone ID Related Accession (Same CDS sequence)	NM_001078838.2
Accession Version	NM_001078838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC136157.1. On Apr 20, 2007 this sequence version replaced NM_001078838.1. ##Evidence-Data-START## Transcript exon combination :: BC136157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028299, SAMD00028300 [ECO:0000348] ##Evidence-Data-END##

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCGTA TGTATACTGT GTCACTTTCG GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072306.2
CDS	44..3151
Translation	MLNNLTECEEGEGGPSQGDGNPKESSPFINSSEMDTGKNMALFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPYVYCVTFGL

Target ORF information:

RefSeq Version	NM_001078838.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 5 (slc12a5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078838.2

ORF Insert Sequence:

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCGTA TGTATACTGT GTCACTTTCG GTCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a5 ( XM_012952549.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808003.1 Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X1, mRNA.

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CloneID	OXa29158
Clone ID Related Accession (Same CDS sequence)	XM_012952549.3 , XM_012952549.2
Accession Version	XM_012952549.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCTGA ATGGGAAAAC TTAAACCAGT CAAATGTACG AAGAATGCAC 
ACAGCTGTGA AACTCAATGA AGTAATTGTG AAAAAATCCC ATGATGCAAA ACTGGTTCTA 
CTAAATATGC CTGGACCACC CCGCAACAGG AAAGGGGACG AAAATTATAT GGAGTTTTTA 
GAAGTCCTCA CAGAGCACCT GGATCGGGTT ATGTTGGTTC GTGGTGGAGG ACGCGAGGTT 
ATTACCATCT ACTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808003.1
CDS	213..3530
Translation	MLNNLTECEEGEGGPSQGDGNPKESSPFINSSEMDTGKNMALFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPEWENLNQSNVRRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_012952549.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952549.2

ORF Insert Sequence:

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCTGA ATGGGAAAAC TTAAACCAGT CAAATGTACG AAGAATGCAC 
ACAGCTGTGA AACTCAATGA AGTAATTGTG AAAAAATCCC ATGATGCAAA ACTGGTTCTA 
CTAAATATGC CTGGACCACC CCGCAACAGG AAAGGGGACG AAAATTATAT GGAGTTTTTA 
GAAGTCCTCA CAGAGCACCT GGATCGGGTT ATGTTGGTTC GTGGTGGAGG ACGCGAGGTT 
ATTACCATCT ACTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a5 ( XM_012952550.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808004.1 Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X2, mRNA.

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CloneID	OXa29159
Clone ID Related Accession (Same CDS sequence)	XM_012952550.1 , XM_012952550.2
Accession Version	XM_012952550.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCAAA CCAGTCAAAT GTACGAAGAA TGCACACAGC TGTGAAACTC 
AATGAAGTAA TTGTGAAAAA ATCCCATGAT GCAAAACTGG TTCTACTAAA TATGCCTGGA 
CCACCCCGCA ACAGGAAAGG GGACGAAAAT TATATGGAGT TTTTAGAAGT CCTCACAGAG 
CACCTGGATC GGGTTATGTT GGTTCGTGGT GGAGGACGCG AGGTTATTAC CATCTACTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808004.1
CDS	212..3514
Translation	MLNNLTECEEGEGGPSQGDGNPKESSPFINSSEMDTGKNMALFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKGFDVCAKFIDSGNETVTTKLWALFCSSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDALNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGNIDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESRGSIRRKNPSNSRLHLSVPEEQVGDCEEKSEEEVQLIHDKNTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDFFNMKPNQSNVRRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_012952550.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 5 (slc12a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952550.1

ORF Insert Sequence:

ATGCTCAATA ACCTGACAGA GTGCGAGGAG GGAGAAGGGG GCCCCAGTCA AGGAGATGGA 
AATCCCAAAG AGAGCTCTCC ATTCATTAAC AGCTCAGAGA TGGACACAGG AAAGAACATG 
GCTCTATTTG AGGAGGAAAT GGACACCAGT CCCATGGTAT CCTCACTCCT CAGCGGCTTG 
GCCAATTACA CAAACCTGAC ACAAGGTGTG AAGGAACACG AGGAAGCAGA GAACAATGAG 
GGCGGAAAGA AGAAGCCAGT CCAGGCACCC CGGATGGGCA CCTTCATGGG GGTCTACCTA 
CCCTGCTTAC AAAACATTTT TGGAGTGATC TTATTCCTAC GTCTCACCTG GGTGGTCGGC 
GTTGGCGGCA TCATGGAATC TTTCATTATG GTTGTGATGT GCTGCTCTTG TACCATGCTG 
ACTGCTATCT CAATGAGCGC CATCGCTACC AATGGAGTTG TGCCAGCTGG TGGATCATAT 
TACATGATCT CTCGATCGCT TGGGCCGGAG TTTGGAGGAG CTGTGGGACT TTGCTTTTAT 
CTTGGAACCA CATTTGCAGG TGCCATGTAC ATCCTGGGAA CCATAGAGAT CCTGCTGACT 
TATCTCTTCC CTGGCATGGC TATCTTTATC GCTCAGGAAG CTAGGGAGGA ACCTGCAGCA 
TTGCTCAACA ATATGCGTGT TTATGGCACA TGTGTGCTCA CCTGCATGGC CATTGTAGTT 
TTTGTAGGAG TTAAATATGT GAATAAGTTT GCCCTGGTTT TCCTGGGCTG TGTCATCTTG 
TCCATCATCG CAATATACGC CGGGGTAATC AAGTCCGCTT TCGACCCTCC AGATTTTCCT 
ATCTGCCTTC TTGGTAATCG TACACTCTCC AGGAAAGGGT TTGATGTCTG TGCCAAGTTT 
ATTGACAGTG GCAACGAGAC AGTCACCACC AAGCTGTGGG CTCTCTTCTG CTCCTCACCA 
TTCCTGAATG CCACATGTGA TACATACTTC TCTCAGAACA ACGTGACTGA GGTTCAGGGC 
ATCCCTGGTG TAGCCAGTGG TCTTATTCGA GATAACCTTT GGGCTGGTTA TATGCAGAAA 
GGTATCATAG TGGAGAAACA AGGACTACGA TCTGCTCCTA ATTCAGATGC CCTCAATCTG 
GATCAGCCTT ATGTTTTCAG TGATATGACC ACCTACTTTA CTCTTCTGGT GGGGATCTAC 
TTCCCCTCAG TTACAGGCAT TATGGCTGGT TCCAACAGGT CTGGAGATCT TCGGGATGCC 
CAGAAATCCA TCCCCATTGG CACTATTATG GCCATAGCCA CCACATCATT TGTGTATATC 
TCTTCAGTTA TTTTATTTGG AGCATGCATC GAGGGGGTTG TCTTGCGTGA CAAATTTGGA 
GAGGCGGTGA ATGGAAATTT AGTGGTAGGC ACTCTAGCAT GGCCATCCCC TTGGGTCATT 
GTGATTGGAT CTTTTTTCTC TACATGTGGG GCAGGTTTGC AGAGTTTAAC AGGGGCCCCA 
CGTCTTCTAC AAGCAATTGC TCGAGATGGA ATTGTTCCGT TTCTCCAGGT CTTTGGTCAT 
GGGAAAGCTA ATGGAGAGCC AACATGGGCC CTTCTCCTCA CCGCCTGTAT CTGTGAGATT 
GGTATCCTCA TTGCATCCCT GGATGAAGTG GCTCCAATTC TTTCAATGTT TTTCTTAATG 
TGTTACATGT TTGTTAACTT GGCCTGTGCA GTGCAGACCT TGTTAAGGAC TCCCAACTGG 
AGGCCTCGTT TCAAATATTA TCACTGGACT CTTTCATTTC TTGGGATGAG TCTTTGTCTG 
TCTCTTATGT TTATCTGCTC CTGGTACTAT GCTCTTGTTG CCATGTCGAT CGCTGGACTT 
ATTTATAAAT ACATTGAGTA CAGAGGGGCA GAGAAAGAGT GGGGTGATGG GATCCGCGGT 
CTGTCCCTCA GTGCTGCTCG ATATGCACTA CTGCGGTTAG AAGAAGGGCC ACCACACACG 
AAGAACTGGA GGCCTCAGTT GCTGGTTCTA GTACGTGTGG ACCAGGATCA AAATGTTGTT 
CACCCGCAGC TCCTTTCATT AACCTCTCAA CTGAAGGCAG GAAAGGGTCT GACGATTGTT 
GGCTCAGTGG TAGAGGGAAC ATACTTAGAA AACCACCCTC AGTGTCAGCG TGCTGAAGAG 
TCTATTCGCC GACTCATGGA GGCAGAGAAA GTTAAAGGTT TCTGCAAGGT GGTTATTTCA 
TCAAATACCC GGGATGGGAT TTCCCATCTG ATTCAGTCTA GTGGACTTGG AGGCTTGCAG 
CACAATACCC TGCTAGTGGG TTGGCCACGA AACTGGAGGC AAAAAGAAGA CCACCAAACC 
TGGAGAAATT TCATTGAACT TGTAAGAGAA ACAACTGCTG CTCACCTGGC TCTAATTGTT 
AGCAAGAATG TAGCTATGTT CCCTGGAAAC CTAGAGAGAT TTTCTGAAGG CAACATTGAT 
GTTTGGTGGG TGGTCCATGA TGGAGGAATG TTGATGTTAC TGCCATTCCT CCTGCGTCAT 
CACAAGGTTT GGAAGAGATG TACAATGCGC ATATTTACTG TTGCACAGAT GGACGACAAC 
AGCATACAGA TGAAGAAAGA CTTGACCACT TTCCTTTATC ATTTAAGAAT TAATGCTGAG 
GTAGAGGTGG TTGAAATGCA TGACAGCGAC ATCTCGGCCT ATACTTATGA GAAGACGCTA 
GTTATGGAGC AGAGGTCCCA AATCCTAAAG CAGATGCATC TCACCAAAAC TGAACGTGAA 
CGTGAGATTC AAAGTATTAC AGATGAATCC CGTGGATCCA TCAGACGTAA GAATCCTTCC 
AACTCCAGGC TGCACTTGAG CGTTCCAGAA GAGCAGGTGG GAGACTGTGA GGAGAAGTCC 
GAAGAAGAGG TACAGCTTAT TCATGACAAA AATACAACAG GCTTTTCAGA GAGTGCCCAG 
TCTCCAGCAG AAGAACCAGA CACAACTCAA GAGAAAGTGC ACCTTACCTG GACTAAAGAC 
AAATGCACAG CAGAAAAAAA CAGTAGTAAA TGTCCCAGCA CTCCAGAGGC CATTAAAGAT 
TTCTTCAACA TGAAGCCAAA CCAGTCAAAT GTACGAAGAA TGCACACAGC TGTGAAACTC 
AATGAAGTAA TTGTGAAAAA ATCCCATGAT GCAAAACTGG TTCTACTAAA TATGCCTGGA 
CCACCCCGCA ACAGGAAAGG GGACGAAAAT TATATGGAGT TTTTAGAAGT CCTCACAGAG 
CACCTGGATC GGGTTATGTT GGTTCGTGGT GGAGGACGCG AGGTTATTAC CATCTACTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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slc12a5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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