kif21b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kif21b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif21b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03842 XM_004910720.4
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa03843 XM_004910721.4
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa03844 XM_004910722.4
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa03845 XM_004910723.4
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29025 XM_012970919.3
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29026 XM_012970924.3
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29027 XM_012970929.2
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29028 XM_012970944.3
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29030 XM_012970955.3
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa29029 XM_012970952.2
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29031 XM_012970961.2
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29032 XM_012970966.2
Latest version!
Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OXa29026 XM_012970924.2 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29030 XM_012970955.2 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa03842 XM_004910720.3 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29028 XM_012970944.2 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa03844 XM_004910722.3 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa03843 XM_004910721.3 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29025 XM_012970919.2 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa29027 XM_012970929.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa03845 XM_004910723.3 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03842
    Clone ID Related Accession (Same CDS sequence) XM_004910720.4 , XM_004910720.3
    Accession Version XM_004910720.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG
    GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT
    GCCACTAGCC TTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910777.1
    CDS185..5062
    Translation

    Target ORF information:

    RefSeq Version XM_004910720.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910720.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG
    GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT
    GCCACTAGCC TTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03843
    Clone ID Related Accession (Same CDS sequence) XM_004910721.4 , XM_004910721.3
    Accession Version XM_004910721.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910721.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910778.1
    CDS185..5059
    Translation

    Target ORF information:

    RefSeq Version XM_004910721.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910721.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03844
    Clone ID Related Accession (Same CDS sequence) XM_004910722.3 , XM_004910722.4
    Accession Version XM_004910722.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910722.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910779.1
    CDS185..5023
    Translation

    Target ORF information:

    RefSeq Version XM_004910722.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910722.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03845
    Clone ID Related Accession (Same CDS sequence) XM_004910723.3 , XM_004910723.4
    Accession Version XM_004910723.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA
    TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA
    CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA
    GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC
    CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA
    TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG
    TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA
    AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC
    CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT
    GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC
    GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT
    TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT
    ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC
    AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC
    TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT
    GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC
    GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC
    TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT
    CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910780.1
    CDS185..5020
    Translation

    Target ORF information:

    RefSeq Version XM_004910723.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910723.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA
    TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA
    CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA
    GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC
    CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA
    TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG
    TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA
    AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC
    CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT
    GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC
    GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT
    TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT
    ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC
    AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC
    TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT
    GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC
    GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC
    TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT
    CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29025
    Clone ID Related Accession (Same CDS sequence) XM_012970919.3 , XM_012970919.2
    Accession Version XM_012970919.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG
    GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA
    AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA
    AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC
    ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA
    CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT
    ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG
    ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA
    ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG
    ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG
    TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826373.1
    CDS185..5059
    Translation

    Target ORF information:

    RefSeq Version XM_012970919.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970919.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG
    GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA
    AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA
    AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC
    ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA
    CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT
    ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG
    ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA
    ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG
    ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG
    TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29026
    Clone ID Related Accession (Same CDS sequence) XM_012970924.3 , XM_012970924.2
    Accession Version XM_012970924.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT
    CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT
    GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT
    GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT
    ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT
    TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG
    CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT
    GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT
    CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC
    AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA
    AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG
    GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA
    TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT
    CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA
    CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA
    CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT
    ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT
    CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT
    ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG
    AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA
    CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA
    GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC
    TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC
    CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG
    CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC
    TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG
    AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT
    CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC
    ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT
    CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG
    TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC
    AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT
    AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT
    AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG
    GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC
    TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT
    TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT
    GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA
    CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC
    CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT
    GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC
    AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC
    AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG
    GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA
    ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA
    GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG
    GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG
    GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT
    GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG
    GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG
    CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA
    GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT
    CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA
    CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA
    TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT
    CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC
    TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG
    TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG
    CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG
    GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC
    TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT
    GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT
    GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT
    TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC
    CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT
    ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT
    GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG
    GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT
    GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA
    GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG
    TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT
    ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC
    ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT
    CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826378.1
    CDS185..5041
    Translation

    Target ORF information:

    RefSeq Version XM_012970924.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT
    CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT
    GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT
    GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT
    ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT
    TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG
    CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT
    GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT
    CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC
    AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA
    AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG
    GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA
    TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT
    CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA
    CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA
    CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT
    ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT
    CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT
    ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG
    AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA
    CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA
    GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC
    TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC
    CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG
    CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC
    TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG
    AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT
    CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC
    ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT
    CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG
    TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC
    AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT
    AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT
    AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG
    GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC
    TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT
    TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT
    GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA
    CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC
    CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT
    GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC
    AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC
    AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG
    GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA
    ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA
    GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG
    GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG
    GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT
    GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG
    GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG
    CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA
    GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT
    CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA
    CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA
    TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT
    CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC
    TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG
    TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG
    CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG
    GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC
    TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT
    GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT
    GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT
    TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC
    CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT
    ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT
    GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG
    GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT
    GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA
    GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG
    TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT
    ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC
    ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT
    CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29027
    Clone ID Related Accession (Same CDS sequence) XM_012970929.2 , XM_012970929.1
    Accession Version XM_012970929.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
    TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG
    TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA
    GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA
    ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC
    CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG
    AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG
    GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT
    ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT
    GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA
    GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC
    CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT
    TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA
    GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG
    AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT
    TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT
    GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA
    AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA
    GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA
    GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA
    CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT
    ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT
    GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA
    CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC
    CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG
    GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA
    AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA
    GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA
    GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA
    GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT
    CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA
    TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA
    ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA
    CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA
    AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC
    AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG
    CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA
    CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG
    CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA
    GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG
    AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT
    CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA
    GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC
    AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG
    AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC
    ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT
    CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG
    CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG
    TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT
    GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA
    GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT
    CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC
    CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA
    CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT
    AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC
    TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA
    TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA
    AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT
    CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA
    TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC
    AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC
    CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA
    CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826383.1
    CDS132..4970
    Translation

    Target ORF information:

    RefSeq Version XM_012970929.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970929.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
    TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG
    TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA
    GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA
    ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC
    CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG
    AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG
    GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT
    ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT
    GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA
    GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC
    CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT
    TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA
    GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG
    AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT
    TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT
    GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA
    AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA
    GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA
    GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA
    CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT
    ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT
    GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA
    CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC
    CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG
    GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA
    AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA
    GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA
    GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA
    GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT
    CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA
    TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA
    ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA
    CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA
    AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC
    AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG
    CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA
    CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG
    CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA
    GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG
    AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT
    CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA
    GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC
    AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG
    AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC
    ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT
    CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG
    CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG
    TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT
    GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA
    GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT
    CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC
    CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA
    CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT
    AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC
    TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA
    TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA
    AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT
    CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA
    TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC
    AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC
    CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA
    CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29028
    Clone ID Related Accession (Same CDS sequence) XM_012970944.3 , XM_012970944.2
    Accession Version XM_012970944.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG
    AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826398.1
    CDS186..5021
    Translation

    Target ORF information:

    RefSeq Version XM_012970944.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970944.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG
    AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29030
    Clone ID Related Accession (Same CDS sequence) XM_012970955.3 , XM_012970955.2
    Accession Version XM_012970955.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT
    GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT
    CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT
    CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG
    TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA
    ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA
    AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG
    AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA
    GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT
    CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC
    CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA
    AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA
    GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA
    GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC
    CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA
    GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG
    AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA
    GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG
    ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG
    ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT
    TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC
    CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT
    GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG
    CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT
    TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG
    TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT
    CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC
    CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT
    ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT
    GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA
    CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA
    AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA
    GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT
    TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA
    GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA
    GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC
    CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT
    GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC
    TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA
    ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT
    TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG
    TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT
    CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT
    AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT
    TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT
    CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT
    TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT
    ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC
    CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT
    CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826409.1
    CDS185..4927
    Translation

    Target ORF information:

    RefSeq Version XM_012970955.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970955.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT
    GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT
    CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT
    CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG
    TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA
    ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA
    AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG
    AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA
    GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT
    CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC
    CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA
    AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA
    GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA
    GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC
    CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA
    GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG
    AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA
    GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG
    ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG
    ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT
    TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC
    CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT
    GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG
    CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT
    TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG
    TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT
    CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC
    CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT
    ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT
    GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA
    CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA
    AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA
    GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT
    TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA
    GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA
    GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC
    CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT
    GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC
    TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA
    ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT
    TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG
    TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT
    CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT
    AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT
    TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT
    CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT
    TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT
    ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC
    CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT
    CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03842
    Clone ID Related Accession (Same CDS sequence) XM_004910720.4 , XM_004910720.3
    Accession Version XM_004910720.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG
    GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT
    GCCACTAGCC TTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910777.1
    CDS190..5067
    Translation

    Target ORF information:

    RefSeq Version XM_004910720.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG
    GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT
    GCCACTAGCC TTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03843
    Clone ID Related Accession (Same CDS sequence) XM_004910721.4 , XM_004910721.3
    Accession Version XM_004910721.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910778.1
    CDS190..5064
    Translation

    Target ORF information:

    RefSeq Version XM_004910721.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910721.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03844
    Clone ID Related Accession (Same CDS sequence) XM_004910722.3 , XM_004910722.4
    Accession Version XM_004910722.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910779.1
    CDS190..5028
    Translation

    Target ORF information:

    RefSeq Version XM_004910722.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910722.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03845
    Clone ID Related Accession (Same CDS sequence) XM_004910723.3 , XM_004910723.4
    Accession Version XM_004910723.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA
    TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA
    CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA
    GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC
    CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA
    TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG
    TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA
    AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC
    CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT
    GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC
    GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT
    TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT
    ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC
    AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC
    TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT
    GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC
    GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC
    TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT
    CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910780.1
    CDS190..5025
    Translation

    Target ORF information:

    RefSeq Version XM_004910723.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910723.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA
    TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA
    CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA
    GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC
    CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA
    TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG
    TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA
    AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC
    CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT
    GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC
    GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT
    TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT
    ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC
    AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC
    TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT
    GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC
    GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC
    TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT
    CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29025
    Clone ID Related Accession (Same CDS sequence) XM_012970919.3 , XM_012970919.2
    Accession Version XM_012970919.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG
    GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA
    AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA
    AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC
    ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA
    CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT
    ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG
    ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA
    ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG
    ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG
    TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826373.1
    CDS190..5064
    Translation

    Target ORF information:

    RefSeq Version XM_012970919.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970919.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG
    GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA
    AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA
    AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC
    ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA
    CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT
    ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG
    ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA
    ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG
    ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG
    TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA
    ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA
    GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT
    GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA
    TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA
    TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC
    AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC
    TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT
    TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC
    AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG
    AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT
    GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG
    AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT
    CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA
    TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG
    TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG
    CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT
    GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG
    GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA
    GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT
    CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG
    AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA
    CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT
    GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC
    ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG
    TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT
    TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC
    ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29026
    Clone ID Related Accession (Same CDS sequence) XM_012970924.3 , XM_012970924.2
    Accession Version XM_012970924.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT
    CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT
    GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT
    GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT
    ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT
    TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG
    CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT
    GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT
    CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC
    AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA
    AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG
    GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA
    TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT
    CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA
    CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA
    CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT
    ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT
    CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT
    ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG
    AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA
    CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA
    GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC
    TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC
    CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG
    CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC
    TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG
    AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT
    CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC
    ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT
    CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG
    TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC
    AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT
    AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT
    AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG
    GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC
    TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT
    TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT
    GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA
    CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC
    CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT
    GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC
    AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC
    AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG
    GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA
    ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA
    GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG
    GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG
    GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT
    GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG
    GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG
    CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA
    GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT
    CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA
    CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA
    TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT
    CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC
    TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG
    TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG
    CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG
    GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC
    TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT
    GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT
    GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT
    TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC
    CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT
    ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT
    GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG
    GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT
    GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA
    GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG
    TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT
    ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC
    ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT
    CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826378.1
    CDS190..5046
    Translation

    Target ORF information:

    RefSeq Version XM_012970924.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT
    CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT
    GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT
    GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT
    ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT
    TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG
    CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT
    GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT
    CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC
    AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA
    AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG
    GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA
    TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT
    CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA
    CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA
    CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT
    ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT
    CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT
    ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG
    AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA
    CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA
    GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC
    TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC
    CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG
    CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC
    TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG
    AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT
    CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC
    ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT
    CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG
    TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC
    AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT
    AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT
    AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG
    GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC
    TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT
    TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT
    GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA
    CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC
    CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT
    GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC
    AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC
    AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG
    GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA
    ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA
    GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG
    GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG
    GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT
    GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG
    GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG
    CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA
    GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT
    CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA
    CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA
    TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT
    CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC
    TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG
    TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG
    CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG
    GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC
    TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT
    GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT
    GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT
    TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC
    CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT
    ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT
    GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG
    GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT
    GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA
    GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG
    TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT
    ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC
    ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT
    CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29027
    Clone ID Related Accession (Same CDS sequence) XM_012970929.2 , XM_012970929.1
    Accession Version XM_012970929.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
    TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG
    TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA
    GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA
    ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC
    CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG
    AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG
    GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT
    ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT
    GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA
    GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC
    CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT
    TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA
    GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG
    AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT
    TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT
    GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA
    AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA
    GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA
    GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA
    CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT
    ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT
    GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA
    CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC
    CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG
    GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA
    AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA
    GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA
    GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA
    GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT
    CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA
    TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA
    ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA
    CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA
    AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC
    AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG
    CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA
    CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG
    CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA
    GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG
    AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT
    CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA
    GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC
    AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG
    AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC
    ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT
    CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG
    CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG
    TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT
    GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA
    GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT
    CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC
    CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA
    CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT
    AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC
    TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA
    TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA
    AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT
    CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA
    TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC
    AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC
    CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA
    CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826383.1
    CDS146..4984
    Translation

    Target ORF information:

    RefSeq Version XM_012970929.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970929.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
    TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG
    TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA
    GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA
    ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC
    CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG
    AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG
    GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT
    ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT
    GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA
    GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC
    CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT
    TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA
    GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG
    AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT
    TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT
    GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA
    AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA
    GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA
    GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA
    CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT
    ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT
    GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA
    CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC
    CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG
    GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA
    AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA
    GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA
    GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA
    GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT
    CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA
    TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA
    ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA
    CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA
    AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC
    AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG
    CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA
    CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG
    CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA
    GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG
    AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT
    CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA
    GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC
    AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG
    AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC
    ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT
    CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG
    CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG
    TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT
    GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA
    GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT
    CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC
    CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA
    CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT
    AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC
    TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA
    TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA
    AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT
    CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA
    TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC
    AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC
    CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA
    CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT
    CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG
    GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT
    AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC
    AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA
    GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA
    TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA
    GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA
    ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC
    ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA
    AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC
    CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT
    GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA
    ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA
    GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT
    GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC
    CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT
    GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT
    GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29028
    Clone ID Related Accession (Same CDS sequence) XM_012970944.3 , XM_012970944.2
    Accession Version XM_012970944.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG
    AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826398.1
    CDS190..5025
    Translation

    Target ORF information:

    RefSeq Version XM_012970944.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970944.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA
    ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG
    TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC
    AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC
    ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC
    ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA
    GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA
    CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG
    CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA
    TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG
    AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG
    ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG
    AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29029
    Clone ID Related Accession (Same CDS sequence) XM_012970952.2
    Accession Version XM_012970952.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGAAG
    GACAGTTCTT CTTTGGAGTA TGAGACTTTG ACTGCTAAAT TTCACTTTGT GGACCTGGCA
    GGATCTGAGC GCCTCAAGCG TACAGGAGCA ACTGGAGAGA GAGCAAGAGA AGGGATCTCT
    ATAAATTGTG GCCTTCTGGC ACTTGGGAAT GTAATCAGTG CTCTTGGAGA TCAAAGTAAG
    AAGGTTCTTC ATGTTCCATA CAGAGATTCA AAACTGACTC GACTACTGCA GGATTCATTA
    GGTGGTAACA GTCAAACCAT GATGATTGCT TGCGTTTCTC CATCTGATCG TGATTTCATG
    GAAACCTTAA ACACTCTAAA GTATGCAAAT CGTGCACGAA ACATTAAGAA TCGTGTAACA
    GCCAATCAGG AGCAGAGTGG ACAGCAAGTA CAGGCTCTTC GGGCAGAACT AGCCAGAGTA
    AAGTTGGAAT TACAAGAGTA CAAGACAGGT CATCGAGTTG CTGGTAATGA TAGCTTAAGC
    GATATGTGTC AGGAGCTGTC TATGTTACGT AGTGAGAATG CATCATTGCG GCTTCGTGTT
    ACTGCCCTAC AAGAGGCTAT TAGAGCTATC AATGCTCGTG TCACTCGTAT GGCTGGACAA
    GAAGCTGCAC ACTTGGTGCA GCGAGCTGGT GATAGTAATG AAGAAATACA AACTCTCATA
    CAGAACTATA TCCATGAAAT AGAAGAACTC AGAACCCGTC TGGTAGAAAG TGAAGCAATG
    AATGAATCTC TCAGACGCAG TTTGTCCCGT TCAACTGCAC GCTCATTGGT AACAGGACCT
    GCAACCCCTA CCCCATCATT AGAAAAGGAG GCCAATGAAG TTGTTCGAAG AGCAAAAGAA
    GAAGTACAGA ATTTGAAAAA GAAAGGAAGT TCATTACAGT CTGATAACTA CAAAAAGAGC
    TTGAAACAAC AACTGCAAAT CAGTGAAGAG ACAGATTATA ATGAAGGAGA AGAGGAGGAT
    GACCATGAAG AAAGTGAATG CGATGAAGAG GAAAGTGAGG GAAGTGGGGA GAGTGAGGCA
    GAGTCAGACT CAGAACCCGA AGAGAAAGGG TTGGGCTGTT GGGACGCAGG GAAAAAATCC
    AGTGGGCCCC TGTCCTTGTG GGCCCCTCTG ACCCAAGCCG GATCCCTGCA AGCGCCCCCA
    CCCAGTCAGC CCTCACCCAG TTTGCCATTC TGCATGGAGG AAGGCTCACT TGGAGGAGAC
    CTTGCTGACT TGACTTGTGA GATTGAAATT AAGCAGCGTC TGATTGAGGA GTTAGAGCAA
    AGTCAGCGGA GACTACAAAC ACTCAAACTT CAGTATGAGG AAAAATTGCA GCAACTCCAG
    AGTCGGATTC GTGATACACA GCTGGAAAGG GACCGAGTGC TACGGGAACT TAGCTCCATG
    GAGTGTTACA CAGAGGAAAA GGCTAACAAG ATCCGTGCTG ATTATGAGAA AAAGCTTCGG
    GAAATGAATC GAGACTTGCA GAAGCTGCAG TTGGCTCAGA GAGAGCATAC ACGACTCCTT
    AAAAATCAGT CCCGTTATGA ACGTGAACTT AAGAAATTGC AGATGGAACT AAGTGATATG
    AAGAAGGCCA AATCAGCTCT AATGAAGCAG ATGCGGGAAG AACAGCAGCT CAGAAAACTT
    GCAGAATCTA GAAGGAACAA AGAAATAGCT CAGCTGAAGA AGGAGCACCA TAGACAAGAG
    CTTCACATTA GAGCTTTGGA GTCCCAGAAG AGACAGCAAG AGATAGTGCT GAGACGCAAG
    ACCCAAGAGG TTTCTGCTCT GCGGCGTCTC TCTAGGCGAC AAACTGATCG ATCTGGGGGA
    CGAAGCCACT CTGTTGCAGA CTCTGGAGCA GAGTTGTCCA CAAGCACAGC ATCTTCAGAA
    CCAGATAGTT CTTTACGTTC TGTGTCCAGT ATTGTCCGTC ATTGGAACCG AAAGACAAGT
    GGAGACGTTG GGCCAGTGCC CAATGGGAGT CGTACACCAA GAAGGAAAGG GGTACGTCCA
    GGCCTACCAC TCAGTAAGGC TGCTCACATG AAGTGGCAAA CTCTGGAGAG AAGGGTTCAT
    GAAGTCGTCC TACAGAGACT TACGCTTCTT AACTTAGAAA GCGATATGGA AAGACTGTTG
    CGGAAACGTG AGGAGCTGTC ACTTCTGCAG GAAGCTTTGA TAAGTCGTTC CATAGCTCTC
    CCAGAAGACA GGAAAGCAGA GGAAAAGTCA CAGCAGGATG TTGCCGAAGA ACTGGAAGCT
    TTGACAGCCA ATATTGATTA TGTTAATGAC TGCATTCGAG AATGCCAGGC TACCATTGTT
    CAGATGGAGG AAACAAAGGA GGATCTAGAC TCAACAGACA CCTCAGTTGT GATCAGTTCT
    TGTTCTCTAA CTGAGGCACG ACATCTTCTT GATAATTTCC TGAGAGCCTC AATAGATAAG
    GGCCTGCAAG TAGCTCAGAA AGAAGCCCAG ATACGGATTC TGGAAGCCCG GATTCGGCAA
    ACTGACATGG CTGGCCTGTC ACAGGCACAC ATGCTGCTGG AGAAATCAGA GTGCCATTCT
    GTGCAGCAGG CAGAAAACGG GTATGCTAGC ACAGATGAAG AGGTGTCAGA AATTAGCCAG
    ACTTCTGATG GAAGTGCTTC TTTCTCCTCC ATGAAAGGAT CCTGCAGTCA AGATGACTTT
    AAGTGGAAGG GGGAACCACG GCTTTCATGG CAAGTGAAGG CCGTGTCTGC TGAGTCTTTG
    AGTCCGCTGC TTGAAGCTTC CACTAGAAAC ATCACCAAAT CCTTAGCATC ATTAACTGAC
    ATCCTAGAAG ATGTTGGGGG ATTCTCTCCG AGTGGTTTGC GGGGCAGAGC ATCACACAGC
    CTTACTCTTC CAATACGTGG TAATACATTT CCGAGACAAT CCAGAGGTTT CAACACATCC
    CCTGTGACAC GCAGAAAATC ATATGACAGA GGACAGCCAA GGGCTTCAGA CTCAAACCTA
    ACACCTCCAT CATCTCCCCC TATGCGCCCT CGTAATGATA AAAATGTCTT TTCTCGGCTA
    ACAAGCAGTC AGAGTTCAGG CACTGCACTG GATAAGTCTG ATGAGAGTGA CAGCTCTTTC
    TCAGATGTCT CCAGGGGCAT AATAAGTCAA TTTGGAGGGC TAAAGAGCAT GAGGACAGCT
    CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA CATACAAAGC CAGTTCTTTG TGTTGAAGCT
    ACAGATGAGC TCCTCTTTAC AGGATCTAAA GATCGCACAT GCAAACTCTG GAATCTGGTA
    ACAGGGCAGG AAATTGCATC CCTTCGCAAC CATCCCCATA ACGTTGTATC TCTGAAACAT
    TGCCCAAACT CTGGGCTAGT TCTCACAGCA TCTGCTTCTT GTGTGCGAGT ATGGGATATC
    CGTGACAGTG CTCGCTGTGT GAGGACATTG TTGTCATCAG GCCAGGTAAT GTCAGGAGAT
    ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA AGCAGCACTC AGGCAGAGTT GCAAATTAAT
    CAAATAACTT TAAATCCTGA TGGCACAATC CTCTATGCAG CAGCCGGCAA TGCAGTGAGA
    GCTTGGGACC TAAGCAGGTT TCAACCCATT GGAAAGCTAA CAGGACACAT GGCTCCAGTC
    ATGTGTCTTA CAGTGAGCAA CACACAAAAC GGACGAGATC TTGTTATCAC AGGATCTAAA
    GATCACTATG TCAAGATATT TGAACTCCAT TCTGGATGGG GAGGGGCAGT TCCCCCCACT
    TATAACCTGG AACCTCCTCA TTACGATGGT ATTGAGTGCT TGGCTGTACA GAATGGGATC
    CTTTGCAGTG GCTCTCGAGA TAATGGAATC AAAAAGTGGG ATCTACAGCA ACATGAGCTC
    ACTCAGCAAG TGCCAAATGC ACACAGAGAC TGGATTTGTG CTTTAGGATT TGTACCGGGA
    CGTTCAATGT TGCTTAGTGG ATGTCGTGGT GGCATTCTAA AGGTTTGGAA CATGGAGAAC
    TTTACACCTA TTGGGGAGAT CAAAGGCCAC GAAAGCCCCA TTAATGCGAT GTGTTCTAAT
    AGCCGTCACA TCTTCACTGC ATCTAGTGAC TGCCGAGTCA AGCTATGGGT TTATGTTCCT
    GGTCTTACTC CATGTCTCCC TCGACGTGTT CTGGCAATCA AGGGCCGTGC CACTAGCCTT
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826406.1
    CDS190..4935
    Translation

    Target ORF information:

    RefSeq Version XM_012970952.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGAAG
    GACAGTTCTT CTTTGGAGTA TGAGACTTTG ACTGCTAAAT TTCACTTTGT GGACCTGGCA
    GGATCTGAGC GCCTCAAGCG TACAGGAGCA ACTGGAGAGA GAGCAAGAGA AGGGATCTCT
    ATAAATTGTG GCCTTCTGGC ACTTGGGAAT GTAATCAGTG CTCTTGGAGA TCAAAGTAAG
    AAGGTTCTTC ATGTTCCATA CAGAGATTCA AAACTGACTC GACTACTGCA GGATTCATTA
    GGTGGTAACA GTCAAACCAT GATGATTGCT TGCGTTTCTC CATCTGATCG TGATTTCATG
    GAAACCTTAA ACACTCTAAA GTATGCAAAT CGTGCACGAA ACATTAAGAA TCGTGTAACA
    GCCAATCAGG AGCAGAGTGG ACAGCAAGTA CAGGCTCTTC GGGCAGAACT AGCCAGAGTA
    AAGTTGGAAT TACAAGAGTA CAAGACAGGT CATCGAGTTG CTGGTAATGA TAGCTTAAGC
    GATATGTGTC AGGAGCTGTC TATGTTACGT AGTGAGAATG CATCATTGCG GCTTCGTGTT
    ACTGCCCTAC AAGAGGCTAT TAGAGCTATC AATGCTCGTG TCACTCGTAT GGCTGGACAA
    GAAGCTGCAC ACTTGGTGCA GCGAGCTGGT GATAGTAATG AAGAAATACA AACTCTCATA
    CAGAACTATA TCCATGAAAT AGAAGAACTC AGAACCCGTC TGGTAGAAAG TGAAGCAATG
    AATGAATCTC TCAGACGCAG TTTGTCCCGT TCAACTGCAC GCTCATTGGT AACAGGACCT
    GCAACCCCTA CCCCATCATT AGAAAAGGAG GCCAATGAAG TTGTTCGAAG AGCAAAAGAA
    GAAGTACAGA ATTTGAAAAA GAAAGGAAGT TCATTACAGT CTGATAACTA CAAAAAGAGC
    TTGAAACAAC AACTGCAAAT CAGTGAAGAG ACAGATTATA ATGAAGGAGA AGAGGAGGAT
    GACCATGAAG AAAGTGAATG CGATGAAGAG GAAAGTGAGG GAAGTGGGGA GAGTGAGGCA
    GAGTCAGACT CAGAACCCGA AGAGAAAGGG TTGGGCTGTT GGGACGCAGG GAAAAAATCC
    AGTGGGCCCC TGTCCTTGTG GGCCCCTCTG ACCCAAGCCG GATCCCTGCA AGCGCCCCCA
    CCCAGTCAGC CCTCACCCAG TTTGCCATTC TGCATGGAGG AAGGCTCACT TGGAGGAGAC
    CTTGCTGACT TGACTTGTGA GATTGAAATT AAGCAGCGTC TGATTGAGGA GTTAGAGCAA
    AGTCAGCGGA GACTACAAAC ACTCAAACTT CAGTATGAGG AAAAATTGCA GCAACTCCAG
    AGTCGGATTC GTGATACACA GCTGGAAAGG GACCGAGTGC TACGGGAACT TAGCTCCATG
    GAGTGTTACA CAGAGGAAAA GGCTAACAAG ATCCGTGCTG ATTATGAGAA AAAGCTTCGG
    GAAATGAATC GAGACTTGCA GAAGCTGCAG TTGGCTCAGA GAGAGCATAC ACGACTCCTT
    AAAAATCAGT CCCGTTATGA ACGTGAACTT AAGAAATTGC AGATGGAACT AAGTGATATG
    AAGAAGGCCA AATCAGCTCT AATGAAGCAG ATGCGGGAAG AACAGCAGCT CAGAAAACTT
    GCAGAATCTA GAAGGAACAA AGAAATAGCT CAGCTGAAGA AGGAGCACCA TAGACAAGAG
    CTTCACATTA GAGCTTTGGA GTCCCAGAAG AGACAGCAAG AGATAGTGCT GAGACGCAAG
    ACCCAAGAGG TTTCTGCTCT GCGGCGTCTC TCTAGGCGAC AAACTGATCG ATCTGGGGGA
    CGAAGCCACT CTGTTGCAGA CTCTGGAGCA GAGTTGTCCA CAAGCACAGC ATCTTCAGAA
    CCAGATAGTT CTTTACGTTC TGTGTCCAGT ATTGTCCGTC ATTGGAACCG AAAGACAAGT
    GGAGACGTTG GGCCAGTGCC CAATGGGAGT CGTACACCAA GAAGGAAAGG GGTACGTCCA
    GGCCTACCAC TCAGTAAGGC TGCTCACATG AAGTGGCAAA CTCTGGAGAG AAGGGTTCAT
    GAAGTCGTCC TACAGAGACT TACGCTTCTT AACTTAGAAA GCGATATGGA AAGACTGTTG
    CGGAAACGTG AGGAGCTGTC ACTTCTGCAG GAAGCTTTGA TAAGTCGTTC CATAGCTCTC
    CCAGAAGACA GGAAAGCAGA GGAAAAGTCA CAGCAGGATG TTGCCGAAGA ACTGGAAGCT
    TTGACAGCCA ATATTGATTA TGTTAATGAC TGCATTCGAG AATGCCAGGC TACCATTGTT
    CAGATGGAGG AAACAAAGGA GGATCTAGAC TCAACAGACA CCTCAGTTGT GATCAGTTCT
    TGTTCTCTAA CTGAGGCACG ACATCTTCTT GATAATTTCC TGAGAGCCTC AATAGATAAG
    GGCCTGCAAG TAGCTCAGAA AGAAGCCCAG ATACGGATTC TGGAAGCCCG GATTCGGCAA
    ACTGACATGG CTGGCCTGTC ACAGGCACAC ATGCTGCTGG AGAAATCAGA GTGCCATTCT
    GTGCAGCAGG CAGAAAACGG GTATGCTAGC ACAGATGAAG AGGTGTCAGA AATTAGCCAG
    ACTTCTGATG GAAGTGCTTC TTTCTCCTCC ATGAAAGGAT CCTGCAGTCA AGATGACTTT
    AAGTGGAAGG GGGAACCACG GCTTTCATGG CAAGTGAAGG CCGTGTCTGC TGAGTCTTTG
    AGTCCGCTGC TTGAAGCTTC CACTAGAAAC ATCACCAAAT CCTTAGCATC ATTAACTGAC
    ATCCTAGAAG ATGTTGGGGG ATTCTCTCCG AGTGGTTTGC GGGGCAGAGC ATCACACAGC
    CTTACTCTTC CAATACGTGG TAATACATTT CCGAGACAAT CCAGAGGTTT CAACACATCC
    CCTGTGACAC GCAGAAAATC ATATGACAGA GGACAGCCAA GGGCTTCAGA CTCAAACCTA
    ACACCTCCAT CATCTCCCCC TATGCGCCCT CGTAATGATA AAAATGTCTT TTCTCGGCTA
    ACAAGCAGTC AGAGTTCAGG CACTGCACTG GATAAGTCTG ATGAGAGTGA CAGCTCTTTC
    TCAGATGTCT CCAGGGGCAT AATAAGTCAA TTTGGAGGGC TAAAGAGCAT GAGGACAGCT
    CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA CATACAAAGC CAGTTCTTTG TGTTGAAGCT
    ACAGATGAGC TCCTCTTTAC AGGATCTAAA GATCGCACAT GCAAACTCTG GAATCTGGTA
    ACAGGGCAGG AAATTGCATC CCTTCGCAAC CATCCCCATA ACGTTGTATC TCTGAAACAT
    TGCCCAAACT CTGGGCTAGT TCTCACAGCA TCTGCTTCTT GTGTGCGAGT ATGGGATATC
    CGTGACAGTG CTCGCTGTGT GAGGACATTG TTGTCATCAG GCCAGGTAAT GTCAGGAGAT
    ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA AGCAGCACTC AGGCAGAGTT GCAAATTAAT
    CAAATAACTT TAAATCCTGA TGGCACAATC CTCTATGCAG CAGCCGGCAA TGCAGTGAGA
    GCTTGGGACC TAAGCAGGTT TCAACCCATT GGAAAGCTAA CAGGACACAT GGCTCCAGTC
    ATGTGTCTTA CAGTGAGCAA CACACAAAAC GGACGAGATC TTGTTATCAC AGGATCTAAA
    GATCACTATG TCAAGATATT TGAACTCCAT TCTGGATGGG GAGGGGCAGT TCCCCCCACT
    TATAACCTGG AACCTCCTCA TTACGATGGT ATTGAGTGCT TGGCTGTACA GAATGGGATC
    CTTTGCAGTG GCTCTCGAGA TAATGGAATC AAAAAGTGGG ATCTACAGCA ACATGAGCTC
    ACTCAGCAAG TGCCAAATGC ACACAGAGAC TGGATTTGTG CTTTAGGATT TGTACCGGGA
    CGTTCAATGT TGCTTAGTGG ATGTCGTGGT GGCATTCTAA AGGTTTGGAA CATGGAGAAC
    TTTACACCTA TTGGGGAGAT CAAAGGCCAC GAAAGCCCCA TTAATGCGAT GTGTTCTAAT
    AGCCGTCACA TCTTCACTGC ATCTAGTGAC TGCCGAGTCA AGCTATGGGT TTATGTTCCT
    GGTCTTACTC CATGTCTCCC TCGACGTGTT CTGGCAATCA AGGGCCGTGC CACTAGCCTT
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29030
    Clone ID Related Accession (Same CDS sequence) XM_012970955.3 , XM_012970955.2
    Accession Version XM_012970955.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT
    GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT
    CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT
    CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG
    TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA
    ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA
    AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG
    AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA
    GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT
    CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC
    CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA
    AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA
    GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA
    GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC
    CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA
    GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG
    AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA
    GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG
    ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG
    ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT
    TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC
    CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT
    GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG
    CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT
    TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG
    TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT
    CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC
    CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT
    ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT
    GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA
    CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA
    AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA
    GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT
    TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA
    GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA
    GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC
    CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT
    GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC
    TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA
    ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT
    TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG
    TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT
    CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT
    AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT
    TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT
    CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT
    TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT
    ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC
    CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT
    CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826409.1
    CDS189..4931
    Translation

    Target ORF information:

    RefSeq Version XM_012970955.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT
    GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT
    CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT
    CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG
    TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA
    ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA
    AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG
    AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA
    GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT
    CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC
    CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA
    AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA
    GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA
    GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC
    CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA
    GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG
    AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA
    GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG
    ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG
    ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT
    TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC
    CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT
    GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG
    CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT
    TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG
    TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT
    CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC
    CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT
    ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT
    GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA
    CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA
    AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA
    GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT
    TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA
    GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA
    GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC
    CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT
    GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC
    TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA
    ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT
    TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG
    TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT
    CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT
    AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT
    TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT
    CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT
    TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT
    ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC
    CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT
    CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29031
    Clone ID Related Accession (Same CDS sequence) XM_012970961.2
    Accession Version XM_012970961.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGGCATA
    ATAAGTCAAT TTGGAGGGCT AAAGAGCATG AGGACAGCTC CTTTGCAGTG TGTTGCAATT
    GCTGAGGGAC ATACAAAGCC AGTTCTTTGT GTTGAAGCTA CAGATGAGCT CCTCTTTACA
    GGATCTAAAG ATCGCACATG CAAACTCTGG AATCTGGTAA CAGGGCAGGA AATTGCATCC
    CTTCGCAACC ATCCCCATAA CGTTGTATCT CTGAAACATT GCCCAAACTC TGGGCTAGTT
    CTCACAGCAT CTGCTTCTTG TGTGCGAGTA TGGGATATCC GTGACAGTGC TCGCTGTGTG
    AGGACATTGT TGTCATCAGG CCAGGTAATG TCAGGAGATA TCTGCTCTGG GCTAGGTTTC
    CGCTCAGGAA GCAGCACTCA GGCAGAGTTG CAAATTAATC AAATAACTTT AAATCCTGAT
    GGCACAATCC TCTATGCAGC AGCCGGCAAT GCAGTGAGAG CTTGGGACCT AAGCAGGTTT
    CAACCCATTG GAAAGCTAAC AGGACACATG GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC
    ACACAAAACG GACGAGATCT TGTTATCACA GGATCTAAAG ATCACTATGT CAAGATATTT
    GAACTCCATT CTGGATGGGG AGGGGCAGTT CCCCCCACTT ATAACCTGGA ACCTCCTCAT
    TACGATGGTA TTGAGTGCTT GGCTGTACAG AATGGGATCC TTTGCAGTGG CTCTCGAGAT
    AATGGAATCA AAAAGTGGGA TCTACAGCAA CATGAGCTCA CTCAGCAAGT GCCAAATGCA
    CACAGAGACT GGATTTGTGC TTTAGGATTT GTACCGGGAC GTTCAATGTT GCTTAGTGGA
    TGTCGTGGTG GCATTCTAAA GGTTTGGAAC ATGGAGAACT TTACACCTAT TGGGGAGATC
    AAAGGCCACG AAAGCCCCAT TAATGCGATG TGTTCTAATA GCCGTCACAT CTTCACTGCA
    TCTAGTGACT GCCGAGTCAA GCTATGGGTT TATGTTCCTG GTCTTACTCC ATGTCTCCCT
    CGACGTGTTC TGGCAATCAA GGGCCGTGCC ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826415.1
    CDS190..4914
    Translation

    Target ORF information:

    RefSeq Version XM_012970961.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970961.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGGCATA
    ATAAGTCAAT TTGGAGGGCT AAAGAGCATG AGGACAGCTC CTTTGCAGTG TGTTGCAATT
    GCTGAGGGAC ATACAAAGCC AGTTCTTTGT GTTGAAGCTA CAGATGAGCT CCTCTTTACA
    GGATCTAAAG ATCGCACATG CAAACTCTGG AATCTGGTAA CAGGGCAGGA AATTGCATCC
    CTTCGCAACC ATCCCCATAA CGTTGTATCT CTGAAACATT GCCCAAACTC TGGGCTAGTT
    CTCACAGCAT CTGCTTCTTG TGTGCGAGTA TGGGATATCC GTGACAGTGC TCGCTGTGTG
    AGGACATTGT TGTCATCAGG CCAGGTAATG TCAGGAGATA TCTGCTCTGG GCTAGGTTTC
    CGCTCAGGAA GCAGCACTCA GGCAGAGTTG CAAATTAATC AAATAACTTT AAATCCTGAT
    GGCACAATCC TCTATGCAGC AGCCGGCAAT GCAGTGAGAG CTTGGGACCT AAGCAGGTTT
    CAACCCATTG GAAAGCTAAC AGGACACATG GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC
    ACACAAAACG GACGAGATCT TGTTATCACA GGATCTAAAG ATCACTATGT CAAGATATTT
    GAACTCCATT CTGGATGGGG AGGGGCAGTT CCCCCCACTT ATAACCTGGA ACCTCCTCAT
    TACGATGGTA TTGAGTGCTT GGCTGTACAG AATGGGATCC TTTGCAGTGG CTCTCGAGAT
    AATGGAATCA AAAAGTGGGA TCTACAGCAA CATGAGCTCA CTCAGCAAGT GCCAAATGCA
    CACAGAGACT GGATTTGTGC TTTAGGATTT GTACCGGGAC GTTCAATGTT GCTTAGTGGA
    TGTCGTGGTG GCATTCTAAA GGTTTGGAAC ATGGAGAACT TTACACCTAT TGGGGAGATC
    AAAGGCCACG AAAGCCCCAT TAATGCGATG TGTTCTAATA GCCGTCACAT CTTCACTGCA
    TCTAGTGACT GCCGAGTCAA GCTATGGGTT TATGTTCCTG GTCTTACTCC ATGTCTCCCT
    CGACGTGTTC TGGCAATCAA GGGCCGTGCC ACTAGCCTTC CATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29032
    Clone ID Related Accession (Same CDS sequence) XM_012970966.2
    Accession Version XM_012970966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTTTCA ACCCATTGGA
    AAGCTAACAG GACACATGGC TCCAGTCATG TGTCTTACAG TGAGCAACAC ACAAAACGGA
    CGAGATCTTG TTATCACAGG ATCTAAAGAT CACTATGTCA AGATATTTGA ACTCCATTCT
    GGATGGGGAG GGGCAGTTCC CCCCACTTAT AACCTGGAAC CTCCTCATTA CGATGGTATT
    GAGTGCTTGG CTGTACAGAA TGGGATCCTT TGCAGTGGCT CTCGAGATAA TGGAATCAAA
    AAGTGGGATC TACAGCAACA TGAGCTCACT CAGCAAGTGC CAAATGCACA CAGAGACTGG
    ATTTGTGCTT TAGGATTTGT ACCGGGACGT TCAATGTTGC TTAGTGGATG TCGTGGTGGC
    ATTCTAAAGG TTTGGAACAT GGAGAACTTT ACACCTATTG GGGAGATCAA AGGCCACGAA
    AGCCCCATTA ATGCGATGTG TTCTAATAGC CGTCACATCT TCACTGCATC TAGTGACTGC
    CGAGTCAAGC TATGGGTTTA TGTTCCTGGT CTTACTCCAT GTCTCCCTCG ACGTGTTCTG
    GCAATCAAGG GCCGTGCCAC TAGCCTTCCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826420.1
    CDS190..4902
    Translation

    Target ORF information:

    RefSeq Version XM_012970966.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
    AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG
    CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA
    ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT
    GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA
    TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT
    TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC
    TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT
    GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT
    AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA
    CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC
    CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG
    ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT
    GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA
    GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA
    GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG
    CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT
    CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG
    AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA
    CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT
    GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG
    CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT
    ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA
    CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA
    AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG
    GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA
    AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC
    TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA
    GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG
    GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA
    GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG
    CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA
    CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG
    GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG
    CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA
    CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG
    AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT
    ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA
    CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG
    CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC
    CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG
    CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT
    CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA
    GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC
    CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA
    GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG
    AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG
    GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT
    TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA
    GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG
    GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT
    GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC
    TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC
    CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA
    GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA
    GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT
    CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT
    GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA
    TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA
    GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT
    TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA
    GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC
    TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT
    GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC
    ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT
    TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC
    TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA
    TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA
    GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTTTCA ACCCATTGGA
    AAGCTAACAG GACACATGGC TCCAGTCATG TGTCTTACAG TGAGCAACAC ACAAAACGGA
    CGAGATCTTG TTATCACAGG ATCTAAAGAT CACTATGTCA AGATATTTGA ACTCCATTCT
    GGATGGGGAG GGGCAGTTCC CCCCACTTAT AACCTGGAAC CTCCTCATTA CGATGGTATT
    GAGTGCTTGG CTGTACAGAA TGGGATCCTT TGCAGTGGCT CTCGAGATAA TGGAATCAAA
    AAGTGGGATC TACAGCAACA TGAGCTCACT CAGCAAGTGC CAAATGCACA CAGAGACTGG
    ATTTGTGCTT TAGGATTTGT ACCGGGACGT TCAATGTTGC TTAGTGGATG TCGTGGTGGC
    ATTCTAAAGG TTTGGAACAT GGAGAACTTT ACACCTATTG GGGAGATCAA AGGCCACGAA
    AGCCCCATTA ATGCGATGTG TTCTAATAGC CGTCACATCT TCACTGCATC TAGTGACTGC
    CGAGTCAAGC TATGGGTTTA TGTTCCTGGT CTTACTCCAT GTCTCCCTCG ACGTGTTCTG
    GCAATCAAGG GCCGTGCCAC TAGCCTTCCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.