gpr158 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following gpr158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpr158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OXa12049 XM_002933144.5
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Xenopus tropicalis G protein-coupled receptor 158 (gpr158), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OXa12049 XM_002933144.4 Xenopus tropicalis G protein-coupled receptor 158 (gpr158), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa12049
    Clone ID Related Accession (Same CDS sequence) XM_002933144.5 , XM_002933144.4
    Accession Version XM_002933144.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable G-protein coupled receptor 158
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933144.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTA GCGTCTCCCT CTCGGCTTGG CTGCTTTGTG TGGGGCTGGT CGCTGCCTCT 
    CGCCGAGACA GCCTGGAAGG GCGCACAGCC GCTCACCCAC AGCAGCACTT CCCAGGCAGG
    TATCCCATCT ACAGCAGCCC TAGCAACCCG CAGCATTCCG CTTTATGGAT TCAAACTACA
    GAGAGTGCCC CCCTGGCACA GACCCTGGCA CAGGATTTAC CCAAACATGT GGCTTCCTTT
    CTGTACAAGG GGGCTGCTGG GGAGCTGAAG GCAGCCTTGT GTTCCCAGAG CTATGAGCTG
    AGCAGCCTGC CAGGGGCATC TCCTGGGCAC CCCACCTTGC TCAGGGCTGT GGATATTCTT
    CTTCATGGCA CCAACTTTTT AAACATGATG TTACAAAGCA ATAAGTCCAG AGAGCACAAC
    TGGGAGAGAG ACGTGGAGTG GTATTACGCC TTGATAAAAA GTATCCTAGA AGGGGAACCC
    GCCATATTCA GGTCGGCCGT CGCCTTCAAC GTGGAGCCCT CGTCCCACGT CCCTCAGGTG
    TTCCTCCGAG CCACCCGAGA GGAAGGCAAG ATACATCTTC AAGATCTGTC AGCGTCGGCC
    CATGGCCCGG GCAATGCCAC GTGGGAACAC GACTGGCTTC ACACCTTCAA ACACAGGTCG
    AGGCCCCACC TGCACAAGAG GCTTCTGCAC GGTGCTAAAA CTTTCGACGA CGGTTGGAAG
    AAAGGGAATA GCTACGTTAC GGACAGGAGT CACGTTAAAT GGTCCTCGCC CTATTTGGAG
    TGCGAAAATG GGAAATACCG CCCTCACTGG CTACTCAGCC TCTCGGCTGC GTTTTATGGT
    CTAAAGCCGA ACCTTGTCCG AGAATTCAGG GGCGTTCTAA AAGTGGATGT AAACCTTCAA
    AACATCGACA TTGACCAGTG CTCAAACGAA GGCTGGTTTT CTGGAACGCA CAGATGCCAG
    CACAACAGCT CAAAGTGTAT ACCAACGAAA GGTCTTGGAT TTGTTCTCGG AGCCTACAAA
    TGCACTTGCA AGGCTGGTTT CTATTATCCC CATGGCATGT TTTCAGAGAA CGGCTTTCAA
    AGGCCGAACA TGGTTAATCA TTTTCCAAGA GGAGAAATAT CTGAGGAAGA ATATAAGTGT
    CTTCCCTGCA GGGAAGGATG TTCTAACTGC ACAGATGATG CTCCCTGCTA TGCACAAGAG
    GACAAGTATT TACGGATTGC TATCATCTCC TTCCAAGCTT TCTGTATGCT CTTGGACTTT
    ATAAGCATGC TAGTAGTTTA CAGCTTTCGC AGAGCAAAGA GTGTTAGAGC ATCAGGACTG
    GTTCTGTTGG AAGTCATTCT TTTTGGGTCA CTTCTCCTAT ACTTCCCAGT CGTCATATTA
    TATTTTGAAC CCAGCACTTT TCGATGCATA CTTCTCAGAT GGGTTCGACT TTTGGGCTAT
    GCAACCGTCT ATGGAACAGT GACACTCAAG CTATACAGGG TGCTGAAGGT GTTTCTGTCT
    CGGACTGCCC AGCGCATTCC CTACATGACA AGCGGCAGAG TTATCCGGAT GCTGTCTGTC
    ATTCTTTTAT TAGTTCTGTG GTTTCTGGTA GCCTGGACTT CAGCAGTGTG CCAGAACATG
    GATAGAAACA TTCCATTAAT TGTTCAAGGA GAAACATCAG ATAACCTACA ATTTAAAATG
    TGTCTTATAG ACCGCTGGGA TTATATGATG GCTGTTGCTG AATTTCTTTT CCTCCTGTGG
    GGGGTGTACC TCTGTTATGC AGTTCGGACA GTTCCATCTG CTTTTCATGA GCCCCGTTAC
    ATGGCTTTTG CAGTCCACAA TGAGCTTATT TTCTCAGCAC TATTCCATAC CATAAGGTTT
    GTTCTTGCAT CAAAACTTCA ACCAGACTGG ATGCTGATGC TGTTTTTTGT ACACACTCAC
    CTGACTGTGA CCGTAACAGT CGGACTGCTG CTTATACCTA AGTTTTCACG GTCACACAAC
    AACCCCAGAG ATGACATAGC AGCCGAGGCA TATGAAGATG AATTAGACTT GGGCAGATCT
    GGCTCATATC TCAATAGCAG TATCACTTCT GCATGGAGTG AACATAGCTT GGATCCTGAA
    GATATAAGGG AAGAACTTAA AAAACTATAC ACCCAACTGG AGATCTATAA GAGGAAGAAA
    ATGATTGCAA ATAACCCTCA TCTTCAGAAA AAGAGGTGTT CCAAGAAAGG ACTGGGGAGA
    TCAATTATGA GGCGAATTAC AGAGATACCA GACTCCGTTA GCAGACAGTG TTCAAAAGAG
    GAAAAAGACA CTACAGATCA TGGCAACCCC AAAAACAATG TTGGCAGCAG TAAAAAACAT
    CCTCAAGAAT CTGTAACGCA TAATGCGAAA TCAAAGGAGG AACCCTCAAA ACATAAACTG
    TATTCTATGA AAAAGTCCCA TAGTAGCTAT GACCATCTGA GGTCTGAAAG TGATACTCCC
    AACGGAATTT CCACCGAGAA AGTAGATGTA TGTAAAAATT CAATATATAG CCCTGTAAAT
    GGGAAGAAGC CTAATAAAAA TGGTTCAGAG CTTGAAGCAG TATCTGCTGA CAATGTTCCT
    TTGGTTTGCA AATCTGCCAG TGCTCACAAT CTGAGCGTGG ATAAGAAGCC TTTGCATCCC
    AGCTTGTCTG TCTTGCAAAA ATCCTTAAGT GCAGTCGCCA ACTCTAAGGA TAACAGCTTG
    GGACTAAGGG ACAAAACACA AAGTGTAGAA GACACTGATA AATGCCATAG AACAGACTCT
    GCGTACCATG AGCATCAACC CTCCGTAGCT ACCTTTGACA AAGAGGAACG TCAGCAGCAT
    ACATCAAGTC CTATCGAGGA GATTCAGAAA CTCCACAAAT CTGGAATAAT GAAACAACAA
    GCAATGAGCC CGCATTTACC AGAATCTGAA AAATTTATCA CTGCTATGGG ATACAAAGAT
    AAGTTTGACA TTGAAGAAGT GTGCCCATGG GAGATGTATG ATCTTCCCCC CTCTGTGCCT
    TCTGAGAATA AAGTTCAGAA ACACGTTTCA ATTGCACCAC TGGAATCAGA AAAGAATCAC
    ACATCTCGCT CAAAAAGTAA ATCCCACAAC CGTTCCAAAA GCGGTGAGCA TGGACATCAG
    CAATCGAAGC AAAAGGGGCA CACAAAAACA GAGCCAAATA TTAGGGGAAC GCAAGAACAG
    CTCTCAAAGG AGGAACAGTT CAAGCAACTT CCTACTAATG ATGCTAATAC AATGGATCAA
    CAGGCAGCGA CAAAACAAAC AACGAAATCA CATGAAAAAG AGGGCAAAAA AAATATTGCA
    CTGGTTGAAG GTGTTTTAAC CTCACCAGAA CCCCACCATA ATTCTAATAA TAACTTGCCA
    CAACCTTTAG TTTTGAGGGC TGAAGTTTGT CCCTGGGACT TTGAAAACCC CGATGTTTTA
    AGTGTAGAAA AAAGCAAGAC TTCGCCTACC ACTACTGTTC TAAGCCCAAC ATCACCATCA
    AGAAACATGG CAACCTCTCC TTTGAAAAAG AAGGTCCTTG GGATGCCAAT AAAAGTTACA
    TCTGCAAATA AAGACGCAGG AACTAAGACA AAAGATTTGG ATCCTCTGGG ATCAATGCTT
    AAGATCGATA AATCGAAAAG TGCAGAAGTG TGTGCTTCTG CAGGGGACAT GACTCTGGGA
    GTGGAAACAA ACAAAGTGAA GAGTTCTTCA AACGAGCATG GTATAGACAG ATGCAGGTTA
    GAAGAAATAT GCCCTTGGGA GAGCCTCTCT CCTTCACAGT CGGAGCAAGA CACACTAGCA
    TCAGATGCTA AACACAACAC AACAATGCAA GGTTCAAATA TGGTGGAAAT CTGTCCATGG
    GACTTTGAAG ATGACAGCAA AGGGAAAGAA GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933190.2
    CDS223..4098
    Translation

    Target ORF information:

    RefSeq Version XM_002933144.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis G protein-coupled receptor 158 (gpr158), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933144.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTA GCGTCTCCCT CTCGGCTTGG CTGCTTTGTG TGGGGCTGGT CGCTGCCTCT 
    CGCCGAGACA GCCTGGAAGG GCGCACAGCC GCTCACCCAC AGCAGCACTT CCCAGGCAGG
    TATCCCATCT ACAGCAGCCC TAGCAACCCG CAGCATTCCG CTTTATGGAT TCAAACTACA
    GAGAGTGCCC CCCTGGCACA GACCCTGGCA CAGGATTTAC CCAAACATGT GGCTTCCTTT
    CTGTACAAGG GGGCTGCTGG GGAGCTGAAG GCAGCCTTGT GTTCCCAGAG CTATGAGCTG
    AGCAGCCTGC CAGGGGCATC TCCTGGGCAC CCCACCTTGC TCAGGGCTGT GGATATTCTT
    CTTCATGGCA CCAACTTTTT AAACATGATG TTACAAAGCA ATAAGTCCAG AGAGCACAAC
    TGGGAGAGAG ACGTGGAGTG GTATTACGCC TTGATAAAAA GTATCCTAGA AGGGGAACCC
    GCCATATTCA GGTCGGCCGT CGCCTTCAAC GTGGAGCCCT CGTCCCACGT CCCTCAGGTG
    TTCCTCCGAG CCACCCGAGA GGAAGGCAAG ATACATCTTC AAGATCTGTC AGCGTCGGCC
    CATGGCCCGG GCAATGCCAC GTGGGAACAC GACTGGCTTC ACACCTTCAA ACACAGGTCG
    AGGCCCCACC TGCACAAGAG GCTTCTGCAC GGTGCTAAAA CTTTCGACGA CGGTTGGAAG
    AAAGGGAATA GCTACGTTAC GGACAGGAGT CACGTTAAAT GGTCCTCGCC CTATTTGGAG
    TGCGAAAATG GGAAATACCG CCCTCACTGG CTACTCAGCC TCTCGGCTGC GTTTTATGGT
    CTAAAGCCGA ACCTTGTCCG AGAATTCAGG GGCGTTCTAA AAGTGGATGT AAACCTTCAA
    AACATCGACA TTGACCAGTG CTCAAACGAA GGCTGGTTTT CTGGAACGCA CAGATGCCAG
    CACAACAGCT CAAAGTGTAT ACCAACGAAA GGTCTTGGAT TTGTTCTCGG AGCCTACAAA
    TGCACTTGCA AGGCTGGTTT CTATTATCCC CATGGCATGT TTTCAGAGAA CGGCTTTCAA
    AGGCCGAACA TGGTTAATCA TTTTCCAAGA GGAGAAATAT CTGAGGAAGA ATATAAGTGT
    CTTCCCTGCA GGGAAGGATG TTCTAACTGC ACAGATGATG CTCCCTGCTA TGCACAAGAG
    GACAAGTATT TACGGATTGC TATCATCTCC TTCCAAGCTT TCTGTATGCT CTTGGACTTT
    ATAAGCATGC TAGTAGTTTA CAGCTTTCGC AGAGCAAAGA GTGTTAGAGC ATCAGGACTG
    GTTCTGTTGG AAGTCATTCT TTTTGGGTCA CTTCTCCTAT ACTTCCCAGT CGTCATATTA
    TATTTTGAAC CCAGCACTTT TCGATGCATA CTTCTCAGAT GGGTTCGACT TTTGGGCTAT
    GCAACCGTCT ATGGAACAGT GACACTCAAG CTATACAGGG TGCTGAAGGT GTTTCTGTCT
    CGGACTGCCC AGCGCATTCC CTACATGACA AGCGGCAGAG TTATCCGGAT GCTGTCTGTC
    ATTCTTTTAT TAGTTCTGTG GTTTCTGGTA GCCTGGACTT CAGCAGTGTG CCAGAACATG
    GATAGAAACA TTCCATTAAT TGTTCAAGGA GAAACATCAG ATAACCTACA ATTTAAAATG
    TGTCTTATAG ACCGCTGGGA TTATATGATG GCTGTTGCTG AATTTCTTTT CCTCCTGTGG
    GGGGTGTACC TCTGTTATGC AGTTCGGACA GTTCCATCTG CTTTTCATGA GCCCCGTTAC
    ATGGCTTTTG CAGTCCACAA TGAGCTTATT TTCTCAGCAC TATTCCATAC CATAAGGTTT
    GTTCTTGCAT CAAAACTTCA ACCAGACTGG ATGCTGATGC TGTTTTTTGT ACACACTCAC
    CTGACTGTGA CCGTAACAGT CGGACTGCTG CTTATACCTA AGTTTTCACG GTCACACAAC
    AACCCCAGAG ATGACATAGC AGCCGAGGCA TATGAAGATG AATTAGACTT GGGCAGATCT
    GGCTCATATC TCAATAGCAG TATCACTTCT GCATGGAGTG AACATAGCTT GGATCCTGAA
    GATATAAGGG AAGAACTTAA AAAACTATAC ACCCAACTGG AGATCTATAA GAGGAAGAAA
    ATGATTGCAA ATAACCCTCA TCTTCAGAAA AAGAGGTGTT CCAAGAAAGG ACTGGGGAGA
    TCAATTATGA GGCGAATTAC AGAGATACCA GACTCCGTTA GCAGACAGTG TTCAAAAGAG
    GAAAAAGACA CTACAGATCA TGGCAACCCC AAAAACAATG TTGGCAGCAG TAAAAAACAT
    CCTCAAGAAT CTGTAACGCA TAATGCGAAA TCAAAGGAGG AACCCTCAAA ACATAAACTG
    TATTCTATGA AAAAGTCCCA TAGTAGCTAT GACCATCTGA GGTCTGAAAG TGATACTCCC
    AACGGAATTT CCACCGAGAA AGTAGATGTA TGTAAAAATT CAATATATAG CCCTGTAAAT
    GGGAAGAAGC CTAATAAAAA TGGTTCAGAG CTTGAAGCAG TATCTGCTGA CAATGTTCCT
    TTGGTTTGCA AATCTGCCAG TGCTCACAAT CTGAGCGTGG ATAAGAAGCC TTTGCATCCC
    AGCTTGTCTG TCTTGCAAAA ATCCTTAAGT GCAGTCGCCA ACTCTAAGGA TAACAGCTTG
    GGACTAAGGG ACAAAACACA AAGTGTAGAA GACACTGATA AATGCCATAG AACAGACTCT
    GCGTACCATG AGCATCAACC CTCCGTAGCT ACCTTTGACA AAGAGGAACG TCAGCAGCAT
    ACATCAAGTC CTATCGAGGA GATTCAGAAA CTCCACAAAT CTGGAATAAT GAAACAACAA
    GCAATGAGCC CGCATTTACC AGAATCTGAA AAATTTATCA CTGCTATGGG ATACAAAGAT
    AAGTTTGACA TTGAAGAAGT GTGCCCATGG GAGATGTATG ATCTTCCCCC CTCTGTGCCT
    TCTGAGAATA AAGTTCAGAA ACACGTTTCA ATTGCACCAC TGGAATCAGA AAAGAATCAC
    ACATCTCGCT CAAAAAGTAA ATCCCACAAC CGTTCCAAAA GCGGTGAGCA TGGACATCAG
    CAATCGAAGC AAAAGGGGCA CACAAAAACA GAGCCAAATA TTAGGGGAAC GCAAGAACAG
    CTCTCAAAGG AGGAACAGTT CAAGCAACTT CCTACTAATG ATGCTAATAC AATGGATCAA
    CAGGCAGCGA CAAAACAAAC AACGAAATCA CATGAAAAAG AGGGCAAAAA AAATATTGCA
    CTGGTTGAAG GTGTTTTAAC CTCACCAGAA CCCCACCATA ATTCTAATAA TAACTTGCCA
    CAACCTTTAG TTTTGAGGGC TGAAGTTTGT CCCTGGGACT TTGAAAACCC CGATGTTTTA
    AGTGTAGAAA AAAGCAAGAC TTCGCCTACC ACTACTGTTC TAAGCCCAAC ATCACCATCA
    AGAAACATGG CAACCTCTCC TTTGAAAAAG AAGGTCCTTG GGATGCCAAT AAAAGTTACA
    TCTGCAAATA AAGACGCAGG AACTAAGACA AAAGATTTGG ATCCTCTGGG ATCAATGCTT
    AAGATCGATA AATCGAAAAG TGCAGAAGTG TGTGCTTCTG CAGGGGACAT GACTCTGGGA
    GTGGAAACAA ACAAAGTGAA GAGTTCTTCA AACGAGCATG GTATAGACAG ATGCAGGTTA
    GAAGAAATAT GCCCTTGGGA GAGCCTCTCT CCTTCACAGT CGGAGCAAGA CACACTAGCA
    TCAGATGCTA AACACAACAC AACAATGCAA GGTTCAAATA TGGTGGAAAT CTGTCCATGG
    GACTTTGAAG ATGACAGCAA AGGGAAAGAA GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12049
    Clone ID Related Accession (Same CDS sequence) XM_002933144.5 , XM_002933144.4
    Accession Version XM_002933144.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable G-protein coupled receptor 158
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTA GCGTCTCCCT CTCGGCTTGG CTGCTTTGTG TGGGGCTGGT CGCTGCCTCT 
    CGCCGAGACA GCCTGGAAGG GCGCACAGCC GCTCACCCAC AGCAGCACTT CCCAGGCAGG
    TATCCCATCT ACAGCAGCCC TAGCAACCCG CAGCATTCCG CTTTATGGAT TCAAACTACA
    GAGAGTGCCC CCCTGGCACA GACCCTGGCA CAGGATTTAC CCAAACATGT GGCTTCCTTT
    CTGTACAAGG GGGCTGCTGG GGAGCTGAAG GCAGCCTTGT GTTCCCAGAG CTATGAGCTG
    AGCAGCCTGC CAGGGGCATC TCCTGGGCAC CCCACCTTGC TCAGGGCTGT GGATATTCTT
    CTTCATGGCA CCAACTTTTT AAACATGATG TTACAAAGCA ATAAGTCCAG AGAGCACAAC
    TGGGAGAGAG ACGTGGAGTG GTATTACGCC TTGATAAAAA GTATCCTAGA AGGGGAACCC
    GCCATATTCA GGTCGGCCGT CGCCTTCAAC GTGGAGCCCT CGTCCCACGT CCCTCAGGTG
    TTCCTCCGAG CCACCCGAGA GGAAGGCAAG ATACATCTTC AAGATCTGTC AGCGTCGGCC
    CATGGCCCGG GCAATGCCAC GTGGGAACAC GACTGGCTTC ACACCTTCAA ACACAGGTCG
    AGGCCCCACC TGCACAAGAG GCTTCTGCAC GGTGCTAAAA CTTTCGACGA CGGTTGGAAG
    AAAGGGAATA GCTACGTTAC GGACAGGAGT CACGTTAAAT GGTCCTCGCC CTATTTGGAG
    TGCGAAAATG GGAAATACCG CCCTCACTGG CTACTCAGCC TCTCGGCTGC GTTTTATGGT
    CTAAAGCCGA ACCTTGTCCG AGAATTCAGG GGCGTTCTAA AAGTGGATGT AAACCTTCAA
    AACATCGACA TTGACCAGTG CTCAAACGAA GGCTGGTTTT CTGGAACGCA CAGATGCCAG
    CACAACAGCT CAAAGTGTAT ACCAACGAAA GGTCTTGGAT TTGTTCTCGG AGCCTACAAA
    TGCACTTGCA AGGCTGGTTT CTATTATCCC CATGGCATGT TTTCAGAGAA CGGCTTTCAA
    AGGCCGAACA TGGTTAATCA TTTTCCAAGA GGAGAAATAT CTGAGGAAGA ATATAAGTGT
    CTTCCCTGCA GGGAAGGATG TTCTAACTGC ACAGATGATG CTCCCTGCTA TGCACAAGAG
    GACAAGTATT TACGGATTGC TATCATCTCC TTCCAAGCTT TCTGTATGCT CTTGGACTTT
    ATAAGCATGC TAGTAGTTTA CAGCTTTCGC AGAGCAAAGA GTGTTAGAGC ATCAGGACTG
    GTTCTGTTGG AAGTCATTCT TTTTGGGTCA CTTCTCCTAT ACTTCCCAGT CGTCATATTA
    TATTTTGAAC CCAGCACTTT TCGATGCATA CTTCTCAGAT GGGTTCGACT TTTGGGCTAT
    GCAACCGTCT ATGGAACAGT GACACTCAAG CTATACAGGG TGCTGAAGGT GTTTCTGTCT
    CGGACTGCCC AGCGCATTCC CTACATGACA AGCGGCAGAG TTATCCGGAT GCTGTCTGTC
    ATTCTTTTAT TAGTTCTGTG GTTTCTGGTA GCCTGGACTT CAGCAGTGTG CCAGAACATG
    GATAGAAACA TTCCATTAAT TGTTCAAGGA GAAACATCAG ATAACCTACA ATTTAAAATG
    TGTCTTATAG ACCGCTGGGA TTATATGATG GCTGTTGCTG AATTTCTTTT CCTCCTGTGG
    GGGGTGTACC TCTGTTATGC AGTTCGGACA GTTCCATCTG CTTTTCATGA GCCCCGTTAC
    ATGGCTTTTG CAGTCCACAA TGAGCTTATT TTCTCAGCAC TATTCCATAC CATAAGGTTT
    GTTCTTGCAT CAAAACTTCA ACCAGACTGG ATGCTGATGC TGTTTTTTGT ACACACTCAC
    CTGACTGTGA CCGTAACAGT CGGACTGCTG CTTATACCTA AGTTTTCACG GTCACACAAC
    AACCCCAGAG ATGACATAGC AGCCGAGGCA TATGAAGATG AATTAGACTT GGGCAGATCT
    GGCTCATATC TCAATAGCAG TATCACTTCT GCATGGAGTG AACATAGCTT GGATCCTGAA
    GATATAAGGG AAGAACTTAA AAAACTATAC ACCCAACTGG AGATCTATAA GAGGAAGAAA
    ATGATTGCAA ATAACCCTCA TCTTCAGAAA AAGAGGTGTT CCAAGAAAGG ACTGGGGAGA
    TCAATTATGA GGCGAATTAC AGAGATACCA GACTCCGTTA GCAGACAGTG TTCAAAAGAG
    GAAAAAGACA CTACAGATCA TGGCAACCCC AAAAACAATG TTGGCAGCAG TAAAAAACAT
    CCTCAAGAAT CTGTAACGCA TAATGCGAAA TCAAAGGAGG AACCCTCAAA ACATAAACTG
    TATTCTATGA AAAAGTCCCA TAGTAGCTAT GACCATCTGA GGTCTGAAAG TGATACTCCC
    AACGGAATTT CCACCGAGAA AGTAGATGTA TGTAAAAATT CAATATATAG CCCTGTAAAT
    GGGAAGAAGC CTAATAAAAA TGGTTCAGAG CTTGAAGCAG TATCTGCTGA CAATGTTCCT
    TTGGTTTGCA AATCTGCCAG TGCTCACAAT CTGAGCGTGG ATAAGAAGCC TTTGCATCCC
    AGCTTGTCTG TCTTGCAAAA ATCCTTAAGT GCAGTCGCCA ACTCTAAGGA TAACAGCTTG
    GGACTAAGGG ACAAAACACA AAGTGTAGAA GACACTGATA AATGCCATAG AACAGACTCT
    GCGTACCATG AGCATCAACC CTCCGTAGCT ACCTTTGACA AAGAGGAACG TCAGCAGCAT
    ACATCAAGTC CTATCGAGGA GATTCAGAAA CTCCACAAAT CTGGAATAAT GAAACAACAA
    GCAATGAGCC CGCATTTACC AGAATCTGAA AAATTTATCA CTGCTATGGG ATACAAAGAT
    AAGTTTGACA TTGAAGAAGT GTGCCCATGG GAGATGTATG ATCTTCCCCC CTCTGTGCCT
    TCTGAGAATA AAGTTCAGAA ACACGTTTCA ATTGCACCAC TGGAATCAGA AAAGAATCAC
    ACATCTCGCT CAAAAAGTAA ATCCCACAAC CGTTCCAAAA GCGGTGAGCA TGGACATCAG
    CAATCGAAGC AAAAGGGGCA CACAAAAACA GAGCCAAATA TTAGGGGAAC GCAAGAACAG
    CTCTCAAAGG AGGAACAGTT CAAGCAACTT CCTACTAATG ATGCTAATAC AATGGATCAA
    CAGGCAGCGA CAAAACAAAC AACGAAATCA CATGAAAAAG AGGGCAAAAA AAATATTGCA
    CTGGTTGAAG GTGTTTTAAC CTCACCAGAA CCCCACCATA ATTCTAATAA TAACTTGCCA
    CAACCTTTAG TTTTGAGGGC TGAAGTTTGT CCCTGGGACT TTGAAAACCC CGATGTTTTA
    AGTGTAGAAA AAAGCAAGAC TTCGCCTACC ACTACTGTTC TAAGCCCAAC ATCACCATCA
    AGAAACATGG CAACCTCTCC TTTGAAAAAG AAGGTCCTTG GGATGCCAAT AAAAGTTACA
    TCTGCAAATA AAGACGCAGG AACTAAGACA AAAGATTTGG ATCCTCTGGG ATCAATGCTT
    AAGATCGATA AATCGAAAAG TGCAGAAGTG TGTGCTTCTG CAGGGGACAT GACTCTGGGA
    GTGGAAACAA ACAAAGTGAA GAGTTCTTCA AACGAGCATG GTATAGACAG ATGCAGGTTA
    GAAGAAATAT GCCCTTGGGA GAGCCTCTCT CCTTCACAGT CGGAGCAAGA CACACTAGCA
    TCAGATGCTA AACACAACAC AACAATGCAA GGTTCAAATA TGGTGGAAAT CTGTCCATGG
    GACTTTGAAG ATGACAGCAA AGGGAAAGAA GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933190.2
    CDS357..4232
    Translation

    Target ORF information:

    RefSeq Version XM_002933144.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis G protein-coupled receptor 158 (gpr158), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933144.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTGTA GCGTCTCCCT CTCGGCTTGG CTGCTTTGTG TGGGGCTGGT CGCTGCCTCT 
    CGCCGAGACA GCCTGGAAGG GCGCACAGCC GCTCACCCAC AGCAGCACTT CCCAGGCAGG
    TATCCCATCT ACAGCAGCCC TAGCAACCCG CAGCATTCCG CTTTATGGAT TCAAACTACA
    GAGAGTGCCC CCCTGGCACA GACCCTGGCA CAGGATTTAC CCAAACATGT GGCTTCCTTT
    CTGTACAAGG GGGCTGCTGG GGAGCTGAAG GCAGCCTTGT GTTCCCAGAG CTATGAGCTG
    AGCAGCCTGC CAGGGGCATC TCCTGGGCAC CCCACCTTGC TCAGGGCTGT GGATATTCTT
    CTTCATGGCA CCAACTTTTT AAACATGATG TTACAAAGCA ATAAGTCCAG AGAGCACAAC
    TGGGAGAGAG ACGTGGAGTG GTATTACGCC TTGATAAAAA GTATCCTAGA AGGGGAACCC
    GCCATATTCA GGTCGGCCGT CGCCTTCAAC GTGGAGCCCT CGTCCCACGT CCCTCAGGTG
    TTCCTCCGAG CCACCCGAGA GGAAGGCAAG ATACATCTTC AAGATCTGTC AGCGTCGGCC
    CATGGCCCGG GCAATGCCAC GTGGGAACAC GACTGGCTTC ACACCTTCAA ACACAGGTCG
    AGGCCCCACC TGCACAAGAG GCTTCTGCAC GGTGCTAAAA CTTTCGACGA CGGTTGGAAG
    AAAGGGAATA GCTACGTTAC GGACAGGAGT CACGTTAAAT GGTCCTCGCC CTATTTGGAG
    TGCGAAAATG GGAAATACCG CCCTCACTGG CTACTCAGCC TCTCGGCTGC GTTTTATGGT
    CTAAAGCCGA ACCTTGTCCG AGAATTCAGG GGCGTTCTAA AAGTGGATGT AAACCTTCAA
    AACATCGACA TTGACCAGTG CTCAAACGAA GGCTGGTTTT CTGGAACGCA CAGATGCCAG
    CACAACAGCT CAAAGTGTAT ACCAACGAAA GGTCTTGGAT TTGTTCTCGG AGCCTACAAA
    TGCACTTGCA AGGCTGGTTT CTATTATCCC CATGGCATGT TTTCAGAGAA CGGCTTTCAA
    AGGCCGAACA TGGTTAATCA TTTTCCAAGA GGAGAAATAT CTGAGGAAGA ATATAAGTGT
    CTTCCCTGCA GGGAAGGATG TTCTAACTGC ACAGATGATG CTCCCTGCTA TGCACAAGAG
    GACAAGTATT TACGGATTGC TATCATCTCC TTCCAAGCTT TCTGTATGCT CTTGGACTTT
    ATAAGCATGC TAGTAGTTTA CAGCTTTCGC AGAGCAAAGA GTGTTAGAGC ATCAGGACTG
    GTTCTGTTGG AAGTCATTCT TTTTGGGTCA CTTCTCCTAT ACTTCCCAGT CGTCATATTA
    TATTTTGAAC CCAGCACTTT TCGATGCATA CTTCTCAGAT GGGTTCGACT TTTGGGCTAT
    GCAACCGTCT ATGGAACAGT GACACTCAAG CTATACAGGG TGCTGAAGGT GTTTCTGTCT
    CGGACTGCCC AGCGCATTCC CTACATGACA AGCGGCAGAG TTATCCGGAT GCTGTCTGTC
    ATTCTTTTAT TAGTTCTGTG GTTTCTGGTA GCCTGGACTT CAGCAGTGTG CCAGAACATG
    GATAGAAACA TTCCATTAAT TGTTCAAGGA GAAACATCAG ATAACCTACA ATTTAAAATG
    TGTCTTATAG ACCGCTGGGA TTATATGATG GCTGTTGCTG AATTTCTTTT CCTCCTGTGG
    GGGGTGTACC TCTGTTATGC AGTTCGGACA GTTCCATCTG CTTTTCATGA GCCCCGTTAC
    ATGGCTTTTG CAGTCCACAA TGAGCTTATT TTCTCAGCAC TATTCCATAC CATAAGGTTT
    GTTCTTGCAT CAAAACTTCA ACCAGACTGG ATGCTGATGC TGTTTTTTGT ACACACTCAC
    CTGACTGTGA CCGTAACAGT CGGACTGCTG CTTATACCTA AGTTTTCACG GTCACACAAC
    AACCCCAGAG ATGACATAGC AGCCGAGGCA TATGAAGATG AATTAGACTT GGGCAGATCT
    GGCTCATATC TCAATAGCAG TATCACTTCT GCATGGAGTG AACATAGCTT GGATCCTGAA
    GATATAAGGG AAGAACTTAA AAAACTATAC ACCCAACTGG AGATCTATAA GAGGAAGAAA
    ATGATTGCAA ATAACCCTCA TCTTCAGAAA AAGAGGTGTT CCAAGAAAGG ACTGGGGAGA
    TCAATTATGA GGCGAATTAC AGAGATACCA GACTCCGTTA GCAGACAGTG TTCAAAAGAG
    GAAAAAGACA CTACAGATCA TGGCAACCCC AAAAACAATG TTGGCAGCAG TAAAAAACAT
    CCTCAAGAAT CTGTAACGCA TAATGCGAAA TCAAAGGAGG AACCCTCAAA ACATAAACTG
    TATTCTATGA AAAAGTCCCA TAGTAGCTAT GACCATCTGA GGTCTGAAAG TGATACTCCC
    AACGGAATTT CCACCGAGAA AGTAGATGTA TGTAAAAATT CAATATATAG CCCTGTAAAT
    GGGAAGAAGC CTAATAAAAA TGGTTCAGAG CTTGAAGCAG TATCTGCTGA CAATGTTCCT
    TTGGTTTGCA AATCTGCCAG TGCTCACAAT CTGAGCGTGG ATAAGAAGCC TTTGCATCCC
    AGCTTGTCTG TCTTGCAAAA ATCCTTAAGT GCAGTCGCCA ACTCTAAGGA TAACAGCTTG
    GGACTAAGGG ACAAAACACA AAGTGTAGAA GACACTGATA AATGCCATAG AACAGACTCT
    GCGTACCATG AGCATCAACC CTCCGTAGCT ACCTTTGACA AAGAGGAACG TCAGCAGCAT
    ACATCAAGTC CTATCGAGGA GATTCAGAAA CTCCACAAAT CTGGAATAAT GAAACAACAA
    GCAATGAGCC CGCATTTACC AGAATCTGAA AAATTTATCA CTGCTATGGG ATACAAAGAT
    AAGTTTGACA TTGAAGAAGT GTGCCCATGG GAGATGTATG ATCTTCCCCC CTCTGTGCCT
    TCTGAGAATA AAGTTCAGAA ACACGTTTCA ATTGCACCAC TGGAATCAGA AAAGAATCAC
    ACATCTCGCT CAAAAAGTAA ATCCCACAAC CGTTCCAAAA GCGGTGAGCA TGGACATCAG
    CAATCGAAGC AAAAGGGGCA CACAAAAACA GAGCCAAATA TTAGGGGAAC GCAAGAACAG
    CTCTCAAAGG AGGAACAGTT CAAGCAACTT CCTACTAATG ATGCTAATAC AATGGATCAA
    CAGGCAGCGA CAAAACAAAC AACGAAATCA CATGAAAAAG AGGGCAAAAA AAATATTGCA
    CTGGTTGAAG GTGTTTTAAC CTCACCAGAA CCCCACCATA ATTCTAATAA TAACTTGCCA
    CAACCTTTAG TTTTGAGGGC TGAAGTTTGT CCCTGGGACT TTGAAAACCC CGATGTTTTA
    AGTGTAGAAA AAAGCAAGAC TTCGCCTACC ACTACTGTTC TAAGCCCAAC ATCACCATCA
    AGAAACATGG CAACCTCTCC TTTGAAAAAG AAGGTCCTTG GGATGCCAAT AAAAGTTACA
    TCTGCAAATA AAGACGCAGG AACTAAGACA AAAGATTTGG ATCCTCTGGG ATCAATGCTT
    AAGATCGATA AATCGAAAAG TGCAGAAGTG TGTGCTTCTG CAGGGGACAT GACTCTGGGA
    GTGGAAACAA ACAAAGTGAA GAGTTCTTCA AACGAGCATG GTATAGACAG ATGCAGGTTA
    GAAGAAATAT GCCCTTGGGA GAGCCTCTCT CCTTCACAGT CGGAGCAAGA CACACTAGCA
    TCAGATGCTA AACACAACAC AACAATGCAA GGTTCAAATA TGGTGGAAAT CTGTCCATGG
    GACTTTGAAG ATGACAGCAA AGGGAAAGAA GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.