rad50 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rad50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54468 XM_018092195.2
Latest version!
Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa54468 XM_012959771.3
Latest version!
Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa07586 XM_012959771.2
Latest version!
Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa07586 XM_018092195.1
Latest version!
Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa07586 XM_018092194.1
Latest version!
Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54468
    Clone ID Related Accession (Same CDS sequence) XM_012959771.3 , XM_018092195.2
    Accession Version XM_012959771.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA repair protein RAD50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815225.1
    CDS172..4110
    Translation

    Target ORF information:

    RefSeq Version XM_012959771.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959771.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54468
    Clone ID Related Accession (Same CDS sequence) XM_012959771.3 , XM_018092195.2
    Accession Version XM_018092195.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA repair protein RAD50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947684.1
    CDS153..4091
    Translation

    Target ORF information:

    RefSeq Version XM_018092195.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07586
    Clone ID Related Accession (Same CDS sequence) XM_018092194.1 , XM_012959771.2 , XM_018092195.1
    Accession Version XM_018092194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA repair protein RAD50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947683.1
    CDS35..3973
    Translation

    Target ORF information:

    RefSeq Version XM_018092194.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07586
    Clone ID Related Accession (Same CDS sequence) XM_018092194.1 , XM_012959771.2 , XM_018092195.1
    Accession Version XM_012959771.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA repair protein RAD50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815225.1
    CDS228..4166
    Translation

    Target ORF information:

    RefSeq Version XM_012959771.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959771.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07586
    Clone ID Related Accession (Same CDS sequence) XM_018092194.1 , XM_012959771.2 , XM_018092195.1
    Accession Version XM_018092195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA repair protein RAD50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947684.1
    CDS209..4147
    Translation

    Target ORF information:

    RefSeq Version XM_018092195.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
    AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA
    GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA
    TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT
    CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG
    ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA
    ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG
    ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA
    GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT
    TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT
    CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA
    AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT
    GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA
    AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA
    ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA
    GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA
    AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC
    AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC
    CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT
    GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG
    AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA
    AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA
    AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC
    AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG
    CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG
    AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG
    CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG
    CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT
    GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG
    TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG
    TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA
    CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT
    GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT
    GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC
    TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT
    ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA
    AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA
    GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA
    ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT
    GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG
    GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC
    CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT
    AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC
    CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC
    CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC
    TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG
    AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA
    CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT
    ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA
    CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG
    ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT
    AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG
    CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG
    GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA
    TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT
    GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT
    CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG
    GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT
    GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA
    ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT
    TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC
    TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA
    AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG
    AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA
    GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG
    AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.