rad50 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad50
Entrez Gene ID	779556
Full Name	RAD50 homolog, double strand break repair protein
General protein information	Preferred Names RAD50 homolog, double strand break repair protein Names DNA repair protein RAD50
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018092194.1	XP_017947683.1	DNA repair protein RAD50
XM_012959771.2	XP_012815225.1	DNA repair protein RAD50
XM_012959771.3	XP_012815225.1	DNA repair protein RAD50
XM_018092195.1	XP_017947684.1	DNA repair protein RAD50
XM_018092195.2	XP_017947684.1	DNA repair protein RAD50

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0497500	NP_001046893.1
Xenopus tropicalis (tropical clawed frog)	rad50	XP_004912891.1
	RAD50	XP_001099384.2
Gallus gallus (chicken)	RAD50	XP_004945072.1
Arabidopsis thaliana (thale cress)	RAD50	NP_565733.1
Homo sapiens (human)	RAD50	NP_005723.2
Rattus norvegicus (Norway rat)	Rad50	NP_071582.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003676	XP_313457.4
Pan troglodytes (chimpanzee)	RAD50	XP_001163412.1
Canis lupus familiaris (dog)	RAD50	XP_531901.2
Neurospora crassa	NCU00901	XP_963818.1
Bos taurus (cattle)	RAD50	NP_001193797.1
Mus musculus (house mouse)	Rad50	NP_033038.2
Danio rerio (zebrafish)	rad50	XP_005167995.1
Drosophila melanogaster (fruit fly)	rad50	NP_001246461.1
Magnaporthe oryzae (rice blast fungus)	MGG_11387	XP_003713570.1

Gene Ontology

The following rad50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54468	XM_018092195.2 Latest version!	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54468	XM_012959771.3 Latest version!	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa07586	XM_012959771.2 Latest version!	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa07586	XM_018092195.1 Latest version!	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa07586	XM_018092194.1 Latest version!	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad50 ( XM_012959771.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815225.1 Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54468
Clone ID Related Accession (Same CDS sequence)	XM_012959771.3 , XM_018092195.2
Accession Version	XM_012959771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815225.1
CDS	172..4110
Translation	MSKIEKMSIQGVRSFGIEDKNKQVIQFFTPLTLLVGPNGAGKTTIIECLKYITTGDFPPGSKGKTFVHDPKVAHETDVRAQIRLQLKDVNGELVAVQRSMICTQKGKSTEFKTLEGVITRMKHGEKVSLSTKCAEMDKEMISALGVSSAVLNNVIFCHQEDSNWPLSEGRQLKVKFDEIFSATRYIKALETLRKVRLNQAQNVREYQVEIKYLKQNKEKAREIQDNLQSKEKQLAVSKENVKSIESQLEPLKDRLADIQRNLTKVMRLDNEIKALESRKRTMEKDNQDLEEKMEKVFQGTDEELSDMYQNHQRTVREKERKLNDHQRDMDRACKESQRLNREKGELLVQQGRLQLEADQHQQYIKTRDSLIKSLAAQLELDGFERTPFNQRQTSNFQMLVKERQEKDEAHANQILREFSEREAMKQRQIDEIRDKKTGLERTIELKSSTQSKKHTDLKNVKYELQQLEGSSDRLQELDEELQKTERELENVEKSCNLEALRGEVAQLQSQKSDLDRNVRKLDQEMEQLNTHTMTRTQMDMLKKDKADKDEQIRKIKSRHNDELSSLIGYFPNKKQLEDWLYSKRKDVNQTREKLARFTKELVAAEQNKNHLSNELRRKEEQSTSFEEKVFDVCGSQDFDSDLSRLQDDIEKTSKQRAMLAGATAVYTQFITTLTEENQPCCPVCQRIFPSEAELQDVINDMQSKLRLVPDKLKAAEGELKRKEKRKDEMMELKPMRQMLADLKEKEVPEIRNKLVAINREIQRLKNDVEEQETLIATFVSEEESAKACLQDISLMERYQMELRDVERKIAQYATKLQGVDLNRTVQQVNQEKQEKQHSLDNVSGKIELLRKRIQDQQEQVQQLKSTVNELTAEKLHISSNLQRRQQLEEQNVELTTELQCLSREIKEAKEQVFPLESTLQKFQQEKQELLQRKELSYREAQEKVNDIKEKVKKINLFSKDIEKYIQDGKEDFKEQKECELQELIARLNECEKQKEKINREMVNIRQDIDTQKIQERCLQDNLTLRKRIEELKRVEEERQQLLKEMGQMKVMQMKNEHQELENKSETLKTNHSLALGRQKGFEDEILRFKKELREPQYKDAEEKYRDKMIVMRTTELAIKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADEGVSAADKRRTYNYRVVMIKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLVVITHDEDFVELLGRSEYVEHFYRIKKNIDQCSEIMRCSVNSLTSYVH

Target ORF information:

RefSeq Version	XM_012959771.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959771.3

ORF Insert Sequence:

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_018092195.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947684.1 Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54468
Clone ID Related Accession (Same CDS sequence)	XM_012959771.3 , XM_018092195.2
Accession Version	XM_018092195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947684.1
CDS	153..4091
Translation	MSKIEKMSIQGVRSFGIEDKNKQVIQFFTPLTLLVGPNGAGKTTIIECLKYITTGDFPPGSKGKTFVHDPKVAHETDVRAQIRLQLKDVNGELVAVQRSMICTQKGKSTEFKTLEGVITRMKHGEKVSLSTKCAEMDKEMISALGVSSAVLNNVIFCHQEDSNWPLSEGRQLKVKFDEIFSATRYIKALETLRKVRLNQAQNVREYQVEIKYLKQNKEKAREIQDNLQSKEKQLAVSKENVKSIESQLEPLKDRLADIQRNLTKVMRLDNEIKALESRKRTMEKDNQDLEEKMEKVFQGTDEELSDMYQNHQRTVREKERKLNDHQRDMDRACKESQRLNREKGELLVQQGRLQLEADQHQQYIKTRDSLIKSLAAQLELDGFERTPFNQRQTSNFQMLVKERQEKDEAHANQILREFSEREAMKQRQIDEIRDKKTGLERTIELKSSTQSKKHTDLKNVKYELQQLEGSSDRLQELDEELQKTERELENVEKSCNLEALRGEVAQLQSQKSDLDRNVRKLDQEMEQLNTHTMTRTQMDMLKKDKADKDEQIRKIKSRHNDELSSLIGYFPNKKQLEDWLYSKRKDVNQTREKLARFTKELVAAEQNKNHLSNELRRKEEQSTSFEEKVFDVCGSQDFDSDLSRLQDDIEKTSKQRAMLAGATAVYTQFITTLTEENQPCCPVCQRIFPSEAELQDVINDMQSKLRLVPDKLKAAEGELKRKEKRKDEMMELKPMRQMLADLKEKEVPEIRNKLVAINREIQRLKNDVEEQETLIATFVSEEESAKACLQDISLMERYQMELRDVERKIAQYATKLQGVDLNRTVQQVNQEKQEKQHSLDNVSGKIELLRKRIQDQQEQVQQLKSTVNELTAEKLHISSNLQRRQQLEEQNVELTTELQCLSREIKEAKEQVFPLESTLQKFQQEKQELLQRKELSYREAQEKVNDIKEKVKKINLFSKDIEKYIQDGKEDFKEQKECELQELIARLNECEKQKEKINREMVNIRQDIDTQKIQERCLQDNLTLRKRIEELKRVEEERQQLLKEMGQMKVMQMKNEHQELENKSETLKTNHSLALGRQKGFEDEILRFKKELREPQYKDAEEKYRDKMIVMRTTELAIKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADEGVSAADKRRTYNYRVVMIKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLVVITHDEDFVELLGRSEYVEHFYRIKKNIDQCSEIMRCSVNSLTSYVH

Target ORF information:

RefSeq Version	XM_018092195.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092195.2

ORF Insert Sequence:

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGCGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTTAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC CCTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCTTT GGCAGAAACA 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACACGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_018092194.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947683.1 Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

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CloneID	OXa07586
Clone ID Related Accession (Same CDS sequence)	XM_018092194.1 , XM_012959771.2 , XM_018092195.1
Accession Version	XM_018092194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947683.1
CDS	35..3973
Translation	MSKIEKMSIQGVRSFGIEDKNKQVIQFFTPLTLLVGPNGAGKTTIIECLKYITTGDFPPGSKGKTFVHDPKVAHETDVRAQIRLQLKDVNGELVAVQRSMICTQKGKSTEFKTLEGVITRMKHGEKVSLSTKCAEMDKEMISALGVSSAVLNNVIFCHQEDSNWPLSEGRQLKVKFDEIFSATRYIKALETLRKVRLNQAQNVREYQVEIKYLKQNKEKAREIQDNLQSKEKQLAVSKENVKSIESQLEPLKDRLADIQRNLTKVMRLDNEIKALESRKRTMEKDNQDLEEKMEKVFQGTDEELSDMYQNHQRTVREKERKLNDHQRDMDRACKESQRLNREKGELLVQQGRLQLEADQHQQYIKTRDSLIKSLAAQLELDGFERTPFNQRQTSNFQMLVKERQEKDEAHANQILREFSEREAMKQRQIDEIRDKKTGLERTIELKSSTQSKKHTDLKNVKYELQQLEGSSDRLQELDEELQKTERELENVEKSCNLEALRGEVAQLQSQKSDLDRNVRKLDQEMEQLNTHTMTRTQMDMLKKDKADKDEQIRKIKSRHNDELSSLIGYFPNKKQLEDWLYSKRKDVNQTREKLARFTKELVAAEQNKNHLSNELRRKEEQSTSFEEKVFDVCGSQDFDSDLSRLQDDIEKTSKQRAMLAGATAVYTQFITTLTEENQPCCPVCQRIFPSEAELQDVINDMQSKLRLVPDKLKAAEGELKRKEKRKDEMMELKPMRQMLADLKEKEVPEIRNKLVAINREIQRLKNDVEEQETLIATFVSEEESAKACLQDISLMERYQMELRDVERKIAQYATKLQGVDLNRTVQQVNQEKQEKQHSLDNVSGKIELLRKRIQDQQEQVQQLKSTVNELTAEKLHISSNLQRRQQLEEQNVELTTELQCLSREIKEAKEQVFPLESTLQKFQQEKQELLQRKELSYREAQEKVNDIKEKVKKINLFSKDIEKYIQDGKEDFKEQKECELQELIARLNECEKQKEKINREMVNIRQDIDTQKIQERCLQDNLTLRKRIEELKRVEEERQQLLKEMGQMKVMQMKNEHQELENKSETLKTNHSLALGRQKGFEDEILRFKKELREPQYKDAEEKYRDKMIVMRTTELAIKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADEGVSAADKRRTYNYRVVMIKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLVVITHDEDFVELLGRSEYVEHFYRIKKNIDQCSEIMRCSVNSLTSYVH

Target ORF information:

RefSeq Version	XM_018092194.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092194.1

ORF Insert Sequence:

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_012959771.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815225.1 Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X3, mRNA.

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CloneID	OXa07586
Clone ID Related Accession (Same CDS sequence)	XM_018092194.1 , XM_012959771.2 , XM_018092195.1
Accession Version	XM_012959771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815225.1
CDS	228..4166
Translation	MSKIEKMSIQGVRSFGIEDKNKQVIQFFTPLTLLVGPNGAGKTTIIECLKYITTGDFPPGSKGKTFVHDPKVAHETDVRAQIRLQLKDVNGELVAVQRSMICTQKGKSTEFKTLEGVITRMKHGEKVSLSTKCAEMDKEMISALGVSSAVLNNVIFCHQEDSNWPLSEGRQLKVKFDEIFSATRYIKALETLRKVRLNQAQNVREYQVEIKYLKQNKEKAREIQDNLQSKEKQLAVSKENVKSIESQLEPLKDRLADIQRNLTKVMRLDNEIKALESRKRTMEKDNQDLEEKMEKVFQGTDEELSDMYQNHQRTVREKERKLNDHQRDMDRACKESQRLNREKGELLVQQGRLQLEADQHQQYIKTRDSLIKSLAAQLELDGFERTPFNQRQTSNFQMLVKERQEKDEAHANQILREFSEREAMKQRQIDEIRDKKTGLERTIELKSSTQSKKHTDLKNVKYELQQLEGSSDRLQELDEELQKTERELENVEKSCNLEALRGEVAQLQSQKSDLDRNVRKLDQEMEQLNTHTMTRTQMDMLKKDKADKDEQIRKIKSRHNDELSSLIGYFPNKKQLEDWLYSKRKDVNQTREKLARFTKELVAAEQNKNHLSNELRRKEEQSTSFEEKVFDVCGSQDFDSDLSRLQDDIEKTSKQRAMLAGATAVYTQFITTLTEENQPCCPVCQRIFPSEAELQDVINDMQSKLRLVPDKLKAAEGELKRKEKRKDEMMELKPMRQMLADLKEKEVPEIRNKLVAINREIQRLKNDVEEQETLIATFVSEEESAKACLQDISLMERYQMELRDVERKIAQYATKLQGVDLNRTVQQVNQEKQEKQHSLDNVSGKIELLRKRIQDQQEQVQQLKSTVNELTAEKLHISSNLQRRQQLEEQNVELTTELQCLSREIKEAKEQVFPLESTLQKFQQEKQELLQRKELSYREAQEKVNDIKEKVKKINLFSKDIEKYIQDGKEDFKEQKECELQELIARLNECEKQKEKINREMVNIRQDIDTQKIQERCLQDNLTLRKRIEELKRVEEERQQLLKEMGQMKVMQMKNEHQELENKSETLKTNHSLALGRQKGFEDEILRFKKELREPQYKDAEEKYRDKMIVMRTTELAIKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADEGVSAADKRRTYNYRVVMIKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLVVITHDEDFVELLGRSEYVEHFYRIKKNIDQCSEIMRCSVNSLTSYVH

Target ORF information:

RefSeq Version	XM_012959771.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959771.2

ORF Insert Sequence:

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_018092195.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947684.1 Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07586
Clone ID Related Accession (Same CDS sequence)	XM_018092194.1 , XM_012959771.2 , XM_018092195.1
Accession Version	XM_018092195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947684.1
CDS	209..4147
Translation	MSKIEKMSIQGVRSFGIEDKNKQVIQFFTPLTLLVGPNGAGKTTIIECLKYITTGDFPPGSKGKTFVHDPKVAHETDVRAQIRLQLKDVNGELVAVQRSMICTQKGKSTEFKTLEGVITRMKHGEKVSLSTKCAEMDKEMISALGVSSAVLNNVIFCHQEDSNWPLSEGRQLKVKFDEIFSATRYIKALETLRKVRLNQAQNVREYQVEIKYLKQNKEKAREIQDNLQSKEKQLAVSKENVKSIESQLEPLKDRLADIQRNLTKVMRLDNEIKALESRKRTMEKDNQDLEEKMEKVFQGTDEELSDMYQNHQRTVREKERKLNDHQRDMDRACKESQRLNREKGELLVQQGRLQLEADQHQQYIKTRDSLIKSLAAQLELDGFERTPFNQRQTSNFQMLVKERQEKDEAHANQILREFSEREAMKQRQIDEIRDKKTGLERTIELKSSTQSKKHTDLKNVKYELQQLEGSSDRLQELDEELQKTERELENVEKSCNLEALRGEVAQLQSQKSDLDRNVRKLDQEMEQLNTHTMTRTQMDMLKKDKADKDEQIRKIKSRHNDELSSLIGYFPNKKQLEDWLYSKRKDVNQTREKLARFTKELVAAEQNKNHLSNELRRKEEQSTSFEEKVFDVCGSQDFDSDLSRLQDDIEKTSKQRAMLAGATAVYTQFITTLTEENQPCCPVCQRIFPSEAELQDVINDMQSKLRLVPDKLKAAEGELKRKEKRKDEMMELKPMRQMLADLKEKEVPEIRNKLVAINREIQRLKNDVEEQETLIATFVSEEESAKACLQDISLMERYQMELRDVERKIAQYATKLQGVDLNRTVQQVNQEKQEKQHSLDNVSGKIELLRKRIQDQQEQVQQLKSTVNELTAEKLHISSNLQRRQQLEEQNVELTTELQCLSREIKEAKEQVFPLESTLQKFQQEKQELLQRKELSYREAQEKVNDIKEKVKKINLFSKDIEKYIQDGKEDFKEQKECELQELIARLNECEKQKEKINREMVNIRQDIDTQKIQERCLQDNLTLRKRIEELKRVEEERQQLLKEMGQMKVMQMKNEHQELENKSETLKTNHSLALGRQKGFEDEILRFKKELREPQYKDAEEKYRDKMIVMRTTELAIKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADEGVSAADKRRTYNYRVVMIKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDRENIESLAHALVEIIKSRSRQRNFQLVVITHDEDFVELLGRSEYVEHFYRIKKNIDQCSEIMRCSVNSLTSYVH

Target ORF information:

RefSeq Version	XM_018092195.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis RAD50 homolog, double strand break repair protein (rad50), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092195.1

ORF Insert Sequence:

ATGTCTAAAA TAGAGAAGAT GAGCATCCAA GGGGTGAGGA GCTTTGGAAT AGAAGATAAA 
AACAAACAAG TTATTCAGTT CTTCACACCT CTAACACTTT TAGTAGGACC CAATGGAGCA 
GGAAAAACAA CTATAATTGA GTGTCTCAAG TATATCACAA CAGGGGATTT CCCACCAGGA 
TCCAAAGGAA AGACATTTGT ACATGATCCT AAGGTCGCTC ATGAAACAGA TGTTCGTGCT 
CAGATTCGTC TACAACTCAA AGATGTTAAT GGAGAACTTG TGGCTGTCCA GCGTTCCATG 
ATCTGCACCC AGAAAGGAAA ATCAACAGAA TTTAAAACAC TTGAAGGGGT TATTACCAGA 
ATGAAGCATG GAGAGAAAGT CAGCCTAAGT ACAAAATGTG CAGAAATGGA TAAGGAAATG 
ATCAGTGCAT TAGGAGTCTC TTCAGCAGTC CTGAATAATG TAATATTCTG CCACCAGGAA 
GACTCCAACT GGCCTTTGAG CGAGGGAAGG CAGTTAAAAG TAAAGTTTGA TGAAATATTT 
TCAGCAACAA GGTATATTAA GGCTTTGGAA ACCTTAAGAA AAGTTCGCTT GAATCAAGCT 
CAGAATGTGA GGGAATATCA GGTAGAAATC AAGTATTTGA AACAGAACAA GGAAAAGGCA 
AGGGAAATTC AAGATAATCT TCAGAGCAAA GAGAAACAGC TTGCTGTTTC CAAAGAAAAT 
GTAAAATCTA TCGAAAGTCA ATTGGAGCCA CTTAAGGATC GCCTGGCAGA CATACAGAGA 
AATCTTACTA AAGTGATGAG ACTTGATAAT GAGATAAAAG CTTTAGAGAG CCGTAAGCGA 
ACCATGGAGA AGGATAACCA AGATTTAGAG GAGAAAATGG AGAAGGTCTT CCAAGGTACA 
GATGAAGAAC TCAGTGATAT GTATCAAAAT CATCAGCGCA CTGTAAGGGA AAAAGAAAGA 
AAGCTGAATG ATCACCAGCG TGACATGGAC AGAGCATGCA AAGAGTCCCA AAGGCTTAAC 
AGGGAAAAAG GAGAACTGCT AGTTCAACAA GGACGTCTGC AGCTGGAGGC AGATCAACAC 
CAGCAATACA TCAAGACAAG GGACTCTCTA ATCAAGTCCC TGGCAGCACA GTTAGAACTT 
GATGGTTTTG AGCGAACTCC TTTTAACCAG AGACAAACAA GCAACTTTCA GATGTTGGTG 
AAAGAAAGGC AGGAAAAGGA TGAGGCCCAT GCAAACCAGA TCTTGAGAGA ATTTTCAGAA 
AGAGAAGCAA TGAAGCAGAG GCAGATCGAT GAAATAAGAG ACAAGAAAAC TGGCCTTGAA 
AGAACTATTG AGCTGAAATC CTCCACACAG AGCAAAAAAC ACACTGACCT GAAGAACGTC 
AAATATGAAC TTCAGCAGTT GGAAGGTTCA TCCGACAGGC TACAAGAACT GGACGAAGAG 
CTTCAGAAGA CGGAGCGTGA ACTGGAGAAT GTGGAAAAGA GCTGCAACTT GGAAGCATTG 
AGAGGGGAAG TTGCCCAACT ACAGAGCCAA AAGTCTGATT TAGACAGAAA TGTACGAAAG 
CTGGATCAGG AGATGGAGCA GCTAAATACA CACACAATGA CAAGAACCCA AATGGACATG 
CTAAAGAAGG ACAAAGCAGA CAAAGATGAA CAAATAAGAA AAATTAAATC TAGACACAAT 
GATGAGTTGA GTTCTTTAAT TGGGTATTTT CCAAATAAGA AGCAACTTGA AGACTGGCTG 
TATTCAAAAA GAAAAGATGT TAATCAGACT CGTGAAAAGC TGGCCCGGTT TACTAAGGAG 
TTAGTAGCAG CTGAGCAGAA CAAAAATCAC CTGAGCAATG AGCTTCGCCG AAAAGAGGAA 
CAGTCCACCT CATTTGAAGA GAAGGTTTTT GATGTGTGTG GCAGCCAGGA CTTTGACAGT 
GATCTGTCAC GCCTCCAGGA CGATATAGAA AAAACTTCTA AACAGAGGGC CATGCTTGCT 
GGAGCAACAG CAGTTTACAC ACAGTTCATT ACCACGCTGA CTGAGGAGAA CCAGCCCTGC 
TGCCCTGTCT GTCAGAGAAT ATTTCCCTCT GAAGCAGAGT TGCAGGATGT GATTAATGAT 
ATGCAATCCA AGCTGCGCCT TGTTCCTGAC AAACTTAAGG CAGCCGAAGG GGAACTGAAA 
AGGAAAGAAA AAAGGAAAGA TGAAATGATG GAACTGAAAC CAATGAGGCA AATGCTGGCA 
GACCTGAAAG AAAAAGAAGT CCCAGAGATA CGAAACAAGC TAGTGGCAAT AAACAGAGAA 
ATACAACGAC TGAAAAATGA TGTAGAAGAG CAGGAAACTC TTATTGCCAC GTTTGTATCT 
GAAGAGGAAA GCGCCAAAGC CTGTTTGCAG GATATCTCCC TCATGGAACG ATATCAGATG 
GAACTTAGAG ATGTAGAGCG GAAAATAGCA CAGTATGCCA CCAAACTACA GGGGGTGGAC 
CTTAACCGCA CTGTCCAGCA GGTTAACCAA GAAAAACAGG AGAAGCAACA CAGCCTGGAT 
AATGTTTCTG GAAAAATTGA GCTTTTAAGA AAACGCATAC AAGATCAGCA AGAACAGGTC 
CAACAGCTGA AAAGTACAGT AAATGAGCTA ACGGCAGAAA AGCTCCACAT CTCAAGTAAC 
CTTCAGCGGC GGCAGCAGTT GGAAGAGCAA AATGTAGAGC TGACAACTGA GCTTCAATGC 
TTATCCAGAG AGATTAAGGA AGCAAAGGAA CAGGTTTTCC CACTGGAATC CACTTTGCAG 
AAATTTCAAC AAGAAAAGCA GGAGCTTCTC CAGAGGAAGG AATTAAGCTA CCGAGAGGCA 
CAGGAAAAAG TTAATGACAT TAAGGAAAAG GTGAAAAAAA TTAATTTATT TTCAAAAGAT 
ATTGAAAAGT ATATTCAAGA CGGAAAGGAA GACTTTAAGG AGCAAAAAGA ATGTGAGCTA 
CAAGAATTAA TTGCGCGACT GAATGAGTGT GAAAAACAGA AGGAGAAAAT AAACAGAGAG 
ATGGTCAATA TCAGACAGGA TATCGACACA CAGAAAATAC AGGAAAGATG TCTTCAAGAT 
AACTTAACTT TGAGAAAAAG GATAGAGGAG CTTAAGCGGG TGGAGGAAGA AAGACAGCAG 
CTTCTAAAAG AAATGGGGCA AATGAAAGTC ATGCAAATGA AAAATGAACA CCAAGAGCTG 
GAGAATAAAA GTGAAACTCT GAAAACCAAC CATAGCCTGG CACTTGGACG ACAGAAAGGA 
TTTGAGGACG AGATCTTGCG ATTTAAGAAG GAATTGAGGG AGCCGCAGTA CAAAGATGCT 
GAAGAAAAAT ACAGAGACAA AATGATTGTA ATGAGAACAA CTGAGCTTGC CATCAAAGAT 
CTGGATATCT ATTATAAAAC ACTGGACCAA GCTATAATGA AGTTCCATAG CATGAAAATG 
GAGGAAATCA ACAAAATTAT CCGTGACCTG TGGCGCAGTA CATACCGAGG CCAAGATATT 
GAGTATATTG AAATCCGATC AGATGCAGAT GAAGGCGTCT CTGCAGCAGA TAAAAGGCGA 
ACATACAATT ATCGTGTAGT GATGATTAAA GGAGACACCG CATTGGATAT GCGAGGACGT 
TGTAGTGCCG GACAAAAGGT CTTGGCTTCC CTTATCATTC GCCTGGCATT GGCAGAAACC 
TTCTGCTTGA ATTGCGGGAT TCTCGCTCTT GACGAACCAA CTACCAACTT GGATCGAGAA 
AATATTGAGT CTCTTGCACA TGCGCTAGTT GAGATCATTA AGAGTCGCTC CAGACAGCGG 
AACTTCCAGC TTGTTGTGAT CACACATGAT GAGGATTTTG TGGAGCTTCT GGGCCGTTCA 
GAATACGTGG AACATTTTTA CAGGATTAAA AAGAACATAG ATCAGTGCTC TGAAATCATG 
AGGTGCAGTG TTAACTCTCT GACATCCTAT GTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rad50 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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