kmt2e gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012960003.2	XP_012815457.1	histone-lysine N-methyltransferase 2E isoform X1
XM_004913017.4	XP_004913074.2	inactive histone-lysine N-methyltransferase 2E isoform X1
XM_018092320.2	XP_017947809.2	inactive histone-lysine N-methyltransferase 2E isoform X2
XM_004913019.4	XP_004913076.2	inactive histone-lysine N-methyltransferase 2E isoform X4
XM_018092321.2	XP_017947810.2	inactive histone-lysine N-methyltransferase 2E isoform X5
XM_018092320.1	XP_017947809.1	histone-lysine N-methyltransferase 2E isoform X2
XM_012960004.2	XP_012815458.1	histone-lysine N-methyltransferase 2E isoform X3
XM_004913017.3	XP_004913074.1	histone-lysine N-methyltransferase 2E isoform X1
XM_018092319.1	XP_017947808.1	histone-lysine N-methyltransferase 2E isoform X1
XM_004913019.3	XP_004913076.1	histone-lysine N-methyltransferase 2E isoform X4
XM_018092321.1	XP_017947810.1	histone-lysine N-methyltransferase 2E isoform X5
XM_012960004.3	XP_012815458.2	inactive histone-lysine N-methyltransferase 2E isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43287	XM_018092320.1 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa43288	XM_018092321.1 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa54848	XM_004913017.4 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa54849	XM_018092320.2 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa54850	XM_012960004.3 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa54851	XM_004913019.4 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa54852	XM_018092321.2 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa07888	XM_004913017.3 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07888	XM_018092319.1 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07888	XM_012960003.2 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa07890	XM_004913019.3 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa28975	XM_012960004.2 Latest version!	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

kmt2e ( XM_018092320.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947809.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa43287
Clone ID Related Accession (Same CDS sequence)	XM_018092320.1
Accession Version	XM_018092320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGGTGACAC CAGTGCAACA GAAAGTTGTG ATGAAGTTCC CATGGAACTG 
TACACTGCTT TCCAGCACAC CCCAACAACT CTTACTCTCA CTGCCACTAG AGTAAATAAG 
GTTAATGACA AAAAGAGGAA GAAAAGTGGT GAGAAGGAGC AGAATAATTC TAAAGTTAAG 
AAGGCTTTTC GTGAAGGATC AAGAAAATCA TCACGTGTTA AGGGCTCTGC TCCAGAAATT 
GATCCCTTAG ACAATTATGC TTTTGGATGG GAGAATAAAA TCAAGACTTG GATGGACCAC 
TATGAAGAAG CAAGCAACAA CCAATACAGT GAAGGGGTTC AAAAAGAGGC ACAAAGGATT 
GCTTTGAGAT TCAGTAACGA AAATGATAAT AAAGAACAAA TAAAGACCAA CTTTAACACC 
AGCAATCTAA TTTTCAAGCC ACCGGTAGAG AGCTATCTAC AAAAGAACAA AAAAATCTTA 
AAGGCTGCCA AAGACTTGCT TCCTGAAACA CTAATTATAG AATACAGGGG AAAATTTATG 
CTCCGGGAGC AGTTTGAAGC AAATGGGTAT TTCTTTAAAA GGCCATACCC TTTTATTTTG 
TTTTATTCTA AATTTCATGG ATTAGAAATG TGTGTTGATG CAAGGACATT TGGAAATGAG 
GCTCGATTTA TACGACGTTC CTGCACACCT AATGCTGAGG TGCGACATGT TATTGAAGAA 
GGAACTATTC ACCTATATAT ATACTCCATT TCAAGCATTC CAAAAGGGGC AGAAATCACT 
ATTGCTTTTG ATTTTGATTA TGCAAATTGT AAATATAAGG TGGATTGTGC ATGCTTCAAA 
GACAATCCAG ACTGCCCAGT TCTCCGATGC AGTACAGAGC CTACAGAAAA TAACAGTGGC 
TATGAAACTA GAAGAAAAAA GGGGAAAAAG GAAAAAGAAC TTTTAAAAGA AAGAGAATCT 
CAAAACCAGA ACATTGTAAT AGATTCAGAT GGAGCAATTA ACAAATTTAA AACATCAGAT 
AACAAGCAAA GAAAGCTCTC CCCTCTTAGA TTGTCAATAT CAAACAACCA GGAGCCAGAT 
ATTAATGATG ATATAGAAGA AAATACTCCT CTTAGCAATG ACGTAGAAAT GGAAACAGAG 
GAGCAGATTG CAGAAAGAAA AAGGAAGATG ACTAGAGAAG AAAGAAAAAT GGAAGCTATT 
CTGCAAGCTT TTGCAAGACT GGAAAAAAGA GAAAAAAGAA GAGAACAAGC TTTAGAAAGA 
ATTGGCACTT CCAAACCAGA CGTGAAAACA GAGAGCAAGG ACTTACAGAT TATCAGTGAA 
TTGGAGCTCC CTCCGGAACC AGTTAAGGAA GAACCTGCCA GCAAACCTAC TCCTGCCAAA 
ATAAATCGCA CAAAGCAGCG AAAGAGTTTT ACAAGAAGTA GGACTCATAT TGGACAACAG 
CGTCGTCGGC ACCGGACTAT AAGTACCTGC TCAGATATCC AGCCATCTTC CCCAGATGTT 
GAGGTTGTAC CACAGCAGAT TGAAACAGAA AATAATGCTG TACCTGAAGA ACCTCCAAAT 
GAAAGCAGTC CCCATAATAC AGAAGAAGTT CCAGAGACAG AATCTTGTGT TCAAAGCAAA 
TGTTCTATAA AGTACCCAAA AACAAAAAAG CACTTGGTAA ATGAATGGTT AAATGAAAAG 
CACGAGAAAG CTGGCAAACC TGGAGAGCCC ATATTGGAAA GACCTTTAAG AATAACCACA 
GATCCTGAAG TCCTTGCTAC TCAGCTGAAT TCCTTACCAG GTTTAATGTA TAGTCCTCAT 
GTGTACACCA CTCCTAAGCA TTATATTAGG TTTACATCAC CATTCCTTTC TGAGAAAAAG 
AAGAAGAAAA ATGAAGAGAA TGTTTCCAGT TGTTGCAAAA AGCGTTGGTT GAAGAAAGCT 
CTGGAAGAAG AAAATTCTTC GGGGGTTAAT CCATTTGGTT CTCCTGCTCA GGACCGATCT 
CAAAGTCCGG TTTCATTACA TGAAAATAAA TGCCATCTAA TAATGAATGG CAGCTCTCCC 
CTGGCAGATT TTACAACTCC ACTGAAGAAA CGAAGGTTGT TTAATTTTTT GGATTGCAAT 
TTTTCCGAAA GTTCAACACC AGTTGCATCT CCTTATGCTA CCCCTACACA TACTGATATT 
ACTTCCATGG ATACATCCTT ATTTACTACT CCACCCAGGG TAAAAGTAGA AGAGGAAATC 
TGTCGCAACG GTTGCAAGCA AACCTACTCA CCAGTTACTC CAGTAACACC CTGTCTGCGT 
GGAAACAACA TGCATTTTGA GAGTATTTCT TCACCTGATA GTTCACCAGA AGTGAACCGA 
AGATCTTTTT GTCAGGAAGG AAATGACCGG GCATCTACAG CATTGAATTC TTTCAGAAAT 
ACCAGTTTGT CTGACATGGG GCTTCAAGAA ATAAAGACTT TTGGCTACTG CAGCCCTAGG 
AATAGGACTG ATGTTTCTGG TCAGTGTCCT GGTGAAAATG ATGCTGCATC AGAGGTCCAT 
TTTGGAGCAG ACATAAATGA ACATAGCTCT CTGCAAAAGG TCATGGAGCC TCCACAGGAT 
AGGACTGATT TAAATAGCCT ACAGGAAACT GCTCTTAGTG GACAAGGCAC CATGTATTCA 
ACTTGGATTA AAAGTCCAAA TAGAACAGGG GTGAACTTCT TGTCCATGAA TTCTAATTTA 
AGAGATTTAA CCCCTTCTCA CCAGCTTGAA GTTGGTGGTG GATTCAGAAT GAATGAATCC 
AAATGCTTGA TCCCAGAAGA CTCACGGGGC GTTTTCATAG ATGGGCCTAT CTTCTGCTCT 
GCAGAGGAGG GAACTGTTTC TGGATTTGGA AGATCTCTTG GTGGTGATGG GATATTAGAC 
GGAAATGGTA CACCACTTAA TCCTCCGCAA AAGAAAAAGG TGTCATTGCT GGAATATAGA 
AAAAGACAGA AAGAAGCAAG AAAAAGTGGC TCTAAACAGG AAAATTTATC TCTGGTCACT 
TTATCTCCTC ATCCGGATGG TATTGTCAAC TGCACCCCTT CTGAAAGTTG TAGTGCTGTT 
GCAGAGTGTA TTGATCAAAA TGACGCTAGC ACTTGTTTAA CTCAGCCATC TGAAGTCCAG 
TCTGTCCCTT CTGAAGAAAC CGAACAAACT TCTTCAGTAA CTGACACCTC ATCCACTGAA 
AAAAATGATC CTGAAGTGCA GTGGACTGCA TCAACATGTG TTGAGCAAGT CAGAGAGAGA 
AGCTATCAAA GAGCTTTGTT ACTGAGTGAC CACAGAAAGG ACAAAGATGA TGCTGACATA 
GAAGGTGGGG ACCCTGCTAA TGCCAAGGAA TGCCAGTCAC CAGACATATC CAGTAAGAAT 
TGTAGAAGTC CTAAACAGAG TAAGCCCTGT TCTCTGGTGC CTAATTTATC TCATGGAAAG 
ACGCCCTCAA AGCTAGATCC TGCCCAAGAA ACCATGGTGA ATCCATCAAC AACTGCTGAT 
GTTAATTTTT GTAACACCGA TTCTCAGCAG AAACAGCTCC TTTCTAAGTC TCTCATTCCC 
CATGCTCAGA AGCTACCTTC TACACCTTTA AAGTTGCATT GTCCGTCATC TCCTCTTCTT 
GAACACCCTC CAAAATCTTC AACACCAGTA CAACATGGTT ATCTTTCACC AAAGCCTCAT 
TCACAAAAAT TAGGTTCTCC ATATAGGCCA CATCATCCCC AGCAGCATCA GGTGGGAACA 
CCACAAAGAG AGTCTCTTCC AAACTTTTAC CCCGTAGTGC AAAGCCTTCA GCCTGCTAAT 
CCACAGCCCT CTAGTGGCCA GTTGTTTTCC CAGACCCAGC CAGGACAGTG CTCCAATGTT 
TATACTCCCT TTAATCAACA GACCGCCAGC AGTCCTGCTC CACCTCCCCC ACCCCCACCT 
CCAACAGCAA CTTACTATCA AAACCAGCAG CCCTCTATAA ACTTCCAGAA CTTTAGTCAG 
ATAAAGGGAG CTGTGTCCCA ACAAACTGTG TTTACACCTG GTCCAAATCA AGGTCATCCA 
ACTGCAGCAG GACAACCAGC AATTCAAGGA CACCATGTAA ACTCTGGACA TTATTTGCCT 
TCTCAAAGCC CTAATGTACA TCTCCAAGCT ACAGGGGTTC CACCACCCCC ACCGCCACCT 
CTTCCAAGCT GCCATCAGCA GCACTCTGTT CAGCATGTTA CAAGCCCAGG TCATACGGTT 
CCAGTAGCCA GTAGCTCACA TATTCATTCT CAGACACCAG GGCATCATTT ACCTCCACCA 
CCCCCTCCAC CACCTGGCCC AAGTCCTCAT CATTCGCATC TTCAGCATGT ATCAGCAGGA 
CACCAAGGCC TGCAAGTGCA GCACCAGCAT GTTTTAAATA CAGTTCCTCC ACCTCCCCCA 
CCACCACCTC CACCCCCTCC ACAAGCAGCA AATGTTTTAG GTGGTGCTGG AAATCACACG 
GCATCAGCAC AAGTTATTCA TATGCAAACT CATCAAGGAC CTGTTTTCTC TTCAGGAGCT 
CATCCTAGTG TAGCACCATA TTCAACACAA GCTTCCCATC ATAACCAAGT TGGACCTGTA 
CCACAGCACC AGTCTTCTGC CACAGGACCG CATCTCCCAC TTCCTGGCCA GAACTCTCAT 
ATTCAGACAC AAGGACCAAA CAGTATTGCA ACACCTGCAG GTTTTTGTCC GCATCCTGGC 
TCTGTAAGCC TTGCTCATGG AGTTCAAGGA CCTCAGCAGG CATCAACAGT GCCTGGCCAG 
GTTCCTATTC ATAGGCCACA GGTACCTCCG ACCTTCCAGA ATAATTACCA TGGCTCAGGA 
TGGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947809.1
CDS	721..6069
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_018092320.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092320.1

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGGTGACAC CAGTGCAACA GAAAGTTGTG ATGAAGTTCC CATGGAACTG 
TACACTGCTT TCCAGCACAC CCCAACAACT CTTACTCTCA CTGCCACTAG AGTAAATAAG 
GTTAATGACA AAAAGAGGAA GAAAAGTGGT GAGAAGGAGC AGAATAATTC TAAAGTTAAG 
AAGGCTTTTC GTGAAGGATC AAGAAAATCA TCACGTGTTA AGGGCTCTGC TCCAGAAATT 
GATCCCTTAG ACAATTATGC TTTTGGATGG GAGAATAAAA TCAAGACTTG GATGGACCAC 
TATGAAGAAG CAAGCAACAA CCAATACAGT GAAGGGGTTC AAAAAGAGGC ACAAAGGATT 
GCTTTGAGAT TCAGTAACGA AAATGATAAT AAAGAACAAA TAAAGACCAA CTTTAACACC 
AGCAATCTAA TTTTCAAGCC ACCGGTAGAG AGCTATCTAC AAAAGAACAA AAAAATCTTA 
AAGGCTGCCA AAGACTTGCT TCCTGAAACA CTAATTATAG AATACAGGGG AAAATTTATG 
CTCCGGGAGC AGTTTGAAGC AAATGGGTAT TTCTTTAAAA GGCCATACCC TTTTATTTTG 
TTTTATTCTA AATTTCATGG ATTAGAAATG TGTGTTGATG CAAGGACATT TGGAAATGAG 
GCTCGATTTA TACGACGTTC CTGCACACCT AATGCTGAGG TGCGACATGT TATTGAAGAA 
GGAACTATTC ACCTATATAT ATACTCCATT TCAAGCATTC CAAAAGGGGC AGAAATCACT 
ATTGCTTTTG ATTTTGATTA TGCAAATTGT AAATATAAGG TGGATTGTGC ATGCTTCAAA 
GACAATCCAG ACTGCCCAGT TCTCCGATGC AGTACAGAGC CTACAGAAAA TAACAGTGGC 
TATGAAACTA GAAGAAAAAA GGGGAAAAAG GAAAAAGAAC TTTTAAAAGA AAGAGAATCT 
CAAAACCAGA ACATTGTAAT AGATTCAGAT GGAGCAATTA ACAAATTTAA AACATCAGAT 
AACAAGCAAA GAAAGCTCTC CCCTCTTAGA TTGTCAATAT CAAACAACCA GGAGCCAGAT 
ATTAATGATG ATATAGAAGA AAATACTCCT CTTAGCAATG ACGTAGAAAT GGAAACAGAG 
GAGCAGATTG CAGAAAGAAA AAGGAAGATG ACTAGAGAAG AAAGAAAAAT GGAAGCTATT 
CTGCAAGCTT TTGCAAGACT GGAAAAAAGA GAAAAAAGAA GAGAACAAGC TTTAGAAAGA 
ATTGGCACTT CCAAACCAGA CGTGAAAACA GAGAGCAAGG ACTTACAGAT TATCAGTGAA 
TTGGAGCTCC CTCCGGAACC AGTTAAGGAA GAACCTGCCA GCAAACCTAC TCCTGCCAAA 
ATAAATCGCA CAAAGCAGCG AAAGAGTTTT ACAAGAAGTA GGACTCATAT TGGACAACAG 
CGTCGTCGGC ACCGGACTAT AAGTACCTGC TCAGATATCC AGCCATCTTC CCCAGATGTT 
GAGGTTGTAC CACAGCAGAT TGAAACAGAA AATAATGCTG TACCTGAAGA ACCTCCAAAT 
GAAAGCAGTC CCCATAATAC AGAAGAAGTT CCAGAGACAG AATCTTGTGT TCAAAGCAAA 
TGTTCTATAA AGTACCCAAA AACAAAAAAG CACTTGGTAA ATGAATGGTT AAATGAAAAG 
CACGAGAAAG CTGGCAAACC TGGAGAGCCC ATATTGGAAA GACCTTTAAG AATAACCACA 
GATCCTGAAG TCCTTGCTAC TCAGCTGAAT TCCTTACCAG GTTTAATGTA TAGTCCTCAT 
GTGTACACCA CTCCTAAGCA TTATATTAGG TTTACATCAC CATTCCTTTC TGAGAAAAAG 
AAGAAGAAAA ATGAAGAGAA TGTTTCCAGT TGTTGCAAAA AGCGTTGGTT GAAGAAAGCT 
CTGGAAGAAG AAAATTCTTC GGGGGTTAAT CCATTTGGTT CTCCTGCTCA GGACCGATCT 
CAAAGTCCGG TTTCATTACA TGAAAATAAA TGCCATCTAA TAATGAATGG CAGCTCTCCC 
CTGGCAGATT TTACAACTCC ACTGAAGAAA CGAAGGTTGT TTAATTTTTT GGATTGCAAT 
TTTTCCGAAA GTTCAACACC AGTTGCATCT CCTTATGCTA CCCCTACACA TACTGATATT 
ACTTCCATGG ATACATCCTT ATTTACTACT CCACCCAGGG TAAAAGTAGA AGAGGAAATC 
TGTCGCAACG GTTGCAAGCA AACCTACTCA CCAGTTACTC CAGTAACACC CTGTCTGCGT 
GGAAACAACA TGCATTTTGA GAGTATTTCT TCACCTGATA GTTCACCAGA AGTGAACCGA 
AGATCTTTTT GTCAGGAAGG AAATGACCGG GCATCTACAG CATTGAATTC TTTCAGAAAT 
ACCAGTTTGT CTGACATGGG GCTTCAAGAA ATAAAGACTT TTGGCTACTG CAGCCCTAGG 
AATAGGACTG ATGTTTCTGG TCAGTGTCCT GGTGAAAATG ATGCTGCATC AGAGGTCCAT 
TTTGGAGCAG ACATAAATGA ACATAGCTCT CTGCAAAAGG TCATGGAGCC TCCACAGGAT 
AGGACTGATT TAAATAGCCT ACAGGAAACT GCTCTTAGTG GACAAGGCAC CATGTATTCA 
ACTTGGATTA AAAGTCCAAA TAGAACAGGG GTGAACTTCT TGTCCATGAA TTCTAATTTA 
AGAGATTTAA CCCCTTCTCA CCAGCTTGAA GTTGGTGGTG GATTCAGAAT GAATGAATCC 
AAATGCTTGA TCCCAGAAGA CTCACGGGGC GTTTTCATAG ATGGGCCTAT CTTCTGCTCT 
GCAGAGGAGG GAACTGTTTC TGGATTTGGA AGATCTCTTG GTGGTGATGG GATATTAGAC 
GGAAATGGTA CACCACTTAA TCCTCCGCAA AAGAAAAAGG TGTCATTGCT GGAATATAGA 
AAAAGACAGA AAGAAGCAAG AAAAAGTGGC TCTAAACAGG AAAATTTATC TCTGGTCACT 
TTATCTCCTC ATCCGGATGG TATTGTCAAC TGCACCCCTT CTGAAAGTTG TAGTGCTGTT 
GCAGAGTGTA TTGATCAAAA TGACGCTAGC ACTTGTTTAA CTCAGCCATC TGAAGTCCAG 
TCTGTCCCTT CTGAAGAAAC CGAACAAACT TCTTCAGTAA CTGACACCTC ATCCACTGAA 
AAAAATGATC CTGAAGTGCA GTGGACTGCA TCAACATGTG TTGAGCAAGT CAGAGAGAGA 
AGCTATCAAA GAGCTTTGTT ACTGAGTGAC CACAGAAAGG ACAAAGATGA TGCTGACATA 
GAAGGTGGGG ACCCTGCTAA TGCCAAGGAA TGCCAGTCAC CAGACATATC CAGTAAGAAT 
TGTAGAAGTC CTAAACAGAG TAAGCCCTGT TCTCTGGTGC CTAATTTATC TCATGGAAAG 
ACGCCCTCAA AGCTAGATCC TGCCCAAGAA ACCATGGTGA ATCCATCAAC AACTGCTGAT 
GTTAATTTTT GTAACACCGA TTCTCAGCAG AAACAGCTCC TTTCTAAGTC TCTCATTCCC 
CATGCTCAGA AGCTACCTTC TACACCTTTA AAGTTGCATT GTCCGTCATC TCCTCTTCTT 
GAACACCCTC CAAAATCTTC AACACCAGTA CAACATGGTT ATCTTTCACC AAAGCCTCAT 
TCACAAAAAT TAGGTTCTCC ATATAGGCCA CATCATCCCC AGCAGCATCA GGTGGGAACA 
CCACAAAGAG AGTCTCTTCC AAACTTTTAC CCCGTAGTGC AAAGCCTTCA GCCTGCTAAT 
CCACAGCCCT CTAGTGGCCA GTTGTTTTCC CAGACCCAGC CAGGACAGTG CTCCAATGTT 
TATACTCCCT TTAATCAACA GACCGCCAGC AGTCCTGCTC CACCTCCCCC ACCCCCACCT 
CCAACAGCAA CTTACTATCA AAACCAGCAG CCCTCTATAA ACTTCCAGAA CTTTAGTCAG 
ATAAAGGGAG CTGTGTCCCA ACAAACTGTG TTTACACCTG GTCCAAATCA AGGTCATCCA 
ACTGCAGCAG GACAACCAGC AATTCAAGGA CACCATGTAA ACTCTGGACA TTATTTGCCT 
TCTCAAAGCC CTAATGTACA TCTCCAAGCT ACAGGGGTTC CACCACCCCC ACCGCCACCT 
CTTCCAAGCT GCCATCAGCA GCACTCTGTT CAGCATGTTA CAAGCCCAGG TCATACGGTT 
CCAGTAGCCA GTAGCTCACA TATTCATTCT CAGACACCAG GGCATCATTT ACCTCCACCA 
CCCCCTCCAC CACCTGGCCC AAGTCCTCAT CATTCGCATC TTCAGCATGT ATCAGCAGGA 
CACCAAGGCC TGCAAGTGCA GCACCAGCAT GTTTTAAATA CAGTTCCTCC ACCTCCCCCA 
CCACCACCTC CACCCCCTCC ACAAGCAGCA AATGTTTTAG GTGGTGCTGG AAATCACACG 
GCATCAGCAC AAGTTATTCA TATGCAAACT CATCAAGGAC CTGTTTTCTC TTCAGGAGCT 
CATCCTAGTG TAGCACCATA TTCAACACAA GCTTCCCATC ATAACCAAGT TGGACCTGTA 
CCACAGCACC AGTCTTCTGC CACAGGACCG CATCTCCCAC TTCCTGGCCA GAACTCTCAT 
ATTCAGACAC AAGGACCAAA CAGTATTGCA ACACCTGCAG GTTTTTGTCC GCATCCTGGC 
TCTGTAAGCC TTGCTCATGG AGTTCAAGGA CCTCAGCAGG CATCAACAGT GCCTGGCCAG 
GTTCCTATTC ATAGGCCACA GGTACCTCCG ACCTTCCAGA ATAATTACCA TGGCTCAGGA 
TGGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_018092321.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947810.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

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CloneID	OXa43288
Clone ID Related Accession (Same CDS sequence)	XM_018092321.1
Accession Version	XM_018092321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGGTGACACC 
AGTGCAACAG AAAGTTGTGA TGAAGTTCCC ATGGAACTGT ACACTGCTTT CCAGCACACC 
CCAACAACTC TTACTCTCAC TGCCACTAGA GTAAATAAGG TTAATGACAA AAAGAGGAAG 
AAAAGTGGTG AGAAGGAGCA GAATAATTCT AAAGTTAAGA AGGCTTTTCG TGAAGGATCA 
AGAAAATCAT CACGTGTTAA GGGCTCTGCT CCAGAAATTG ATCCCTTAGA CAATTATGCT 
TTTGGATGGG AGAATAAAAT CAAGACTTGG ATGGACCACT ATGAAGAAGC AAGCAACAAC 
CAATACAGTG AAGGGGTTCA AAAAGAGGCA CAAAGGATTG CTTTGAGATT CAGTAACGAA 
AATGATAATA AAGAACAAAT AAAGACCAAC TTTAACACCA GCAATCTAAT TTTCAAGCCA 
CCGGTAGAGA GCTATCTACA AAAGAACAAA AAAATCTTAA AGGCTGCCAA AGACTTGCTT 
CCTGAAACAC TAATTATAGA ATACAGGGGA AAATTTATGC TCCGGGAGCA GTTTGAAGCA 
AATGGGTATT TCTTTAAAAG GCCATACCCT TTTATTTTGT TTTATTCTAA ATTTCATGGA 
TTAGAAATGT GTGTTGATGC AAGGACATTT GGAAATGAGG CTCGATTTAT ACGACGTTCC 
TGCACACCTA ATGCTGAGGT GCGACATGTT ATTGAAGAAG GAACTATTCA CCTATATATA 
TACTCCATTT CAAGCATTCC AAAAGGGGCA GAAATCACTA TTGCTTTTGA TTTTGATTAT 
GCAAATTGTA AATATAAGGT GGATTGTGCA TGCTTCAAAG ACAATCCAGA CTGCCCAGTT 
CTCCGATGCA GTACAGAGCC TACAGAAAAT AACAGTGGCT ATGAAACTAG AAGAAAAAAG 
GGGAAAAAGG AAAAAGAACT TTTAAAAGAA AGAGAATCTC AAAACCAGAA CATTGTAATA 
GATTCAGATG GAGCAATTAA CAAATTTAAA ACATCAGATA ACAAGCAAAG AAAGCTCTCC 
CCTCTTAGAT TGTCAATATC AAACAACCAG GAGCCAGATA TTAATGATGA TATAGAAGAA 
AATACTCCTC TTAGCAATGA CGTAGAAATG GAAACAGAGG AGCAGATTGC AGAAAGAAAA 
AGGAAGATGA CTAGAGAAGA AAGAAAAATG GAAGCTATTC TGCAAGCTTT TGCAAGACTG 
GAAAAAAGAG AAAAAAGAAG AGAACAAGCT TTAGAAAGAA TTGGCACTTC CAAACCAGAC 
GTGAAAACAG AGAGCAAGGA CTTACAGATT ATCAGTGAAT TGGAGCTCCC TCCGGAACCA 
GTTAAGGAAG AACCTGCCAG CAAACCTACT CCTGCCAAAA TAAATCGCAC AAAGCAGCGA 
AAGAGTTTTA CAAGAAGTAG GACTCATATT GGACAACAGC GTCGTCGGCA CCGGACTATA 
AGTACCTGCT CAGATATCCA GCCATCTTCC CCAGATGTTG AGGTTGTACC ACAGCAGATT 
GAAACAGAAA ATAATGCTGT ACCTGAAGAA CCTCCAAATG AAAGCAGTCC CCATAATACA 
GAAGAAGTTC CAGAGACAGA ATCTTGTGTT CAAAGCAAAT GTTCTATAAA GTACCCAAAA 
ACAAAAAAGC ACTTGGTAAA TGAATGGTTA AATGAAAAGC ACGAGAAAGC TGGCAAACCT 
GGAGAGCCCA TATTGGAAAG ACCTTTAAGA ATAACCACAG ATCCTGAAGT CCTTGCTACT 
CAGCTGAATT CCTTACCAGG TTTAATGTAT AGTCCTCATG TGTACACCAC TCCTAAGCAT 
TATATTAGGT TTACATCACC ATTCCTTTCT GAGAAAAAGA AGAAGAAAAA TGAAGAGAAT 
GTTTCCAGTT GTTGCAAAAA GCGTTGGTTG AAGAAAGCTC TGGAAGAAGA AAATTCTTCG 
GGGGTTAATC CATTTGGTTC TCCTGCTCAG GACCGATCTC AAAGTCCGGT TTCATTACAT 
GAAAATAAAT GCCATCTAAT AATGAATGGC AGCTCTCCCC TGGCAGATTT TACAACTCCA 
CTGAAGAAAC GAAGGTTGTT TAATTTTTTG GATTGCAATT TTTCCGAAAG TTCAACACCA 
GTTGCATCTC CTTATGCTAC CCCTACACAT ACTGATATTA CTTCCATGGA TACATCCTTA 
TTTACTACTC CACCCAGGGT AAAAGTAGAA GAGGAAATCT GTCGCAACGG TTGCAAGCAA 
ACCTACTCAC CAGTTACTCC AGTAACACCC TGTCTGCGTG GAAACAACAT GCATTTTGAG 
AGTATTTCTT CACCTGATAG TTCACCAGAA GTGAACCGAA GATCTTTTTG TCAGGAAGGA 
AATGACCGGG CATCTACAGC ATTGAATTCT TTCAGAAATA CCAGTTTGTC TGACATGGGG 
CTTCAAGAAA TAAAGACTTT TGGCTACTGC AGCCCTAGGA ATAGGACTGA TGTTTCTGGT 
CAGTGTCCTG GTGAAAATGA TGCTGCATCA GAGGTCCATT TTGGAGCAGA CATAAATGAA 
CATAGCTCTC TGCAAAAGGT CATGGAGCCT CCACAGGATA GGACTGATTT AAATAGCCTA 
CAGGAAACTG CTCTTAGTGG ACAAGGCACC ATGTATTCAA CTTGGATTAA AAGTCCAAAT 
AGAACAGGGG TGAACTTCTT GTCCATGAAT TCTAATTTAA GAGATTTAAC CCCTTCTCAC 
CAGCTTGAAG TTGGTGGTGG ATTCAGAATG AATGAATCCA AATGCTTGAT CCCAGAAGAC 
TCACGGGGCG TTTTCATAGA TGGGCCTATC TTCTGCTCTG CAGAGGAGGG AACTGTTTCT 
GGATTTGGAA GATCTCTTGG TGGTGATGGG ATATTAGACG GAAATGGTAC ACCACTTAAT 
CCTCCGCAAA AGAAAAAGGT GTCATTGCTG GAATATAGAA AAAGACAGAA AGAAGCAAGA 
AAAAGTGGCT CTAAACAGGA AAATTTATCT CTGGTCACTT TATCTCCTCA TCCGGATGGT 
ATTGTCAACT GCACCCCTTC TGAAAGTTGT AGTGCTGTTG CAGAGTGTAT TGATCAAAAT 
GACGCTAGCA CTTGTTTAAC TCAGCCATCT GAAGTCCAGT CTGTCCCTTC TGAAGAAACC 
GAACAAACTT CTTCAGTAAC TGACACCTCA TCCACTGAAA AAAATGATCC TGAAGTGCAG 
TGGACTGCAT CAACATGTGT TGAGCAAGTC AGAGAGAGAA GCTATCAAAG AGCTTTGTTA 
CTGAGTGACC ACAGAAAGGA CAAAGATGAT GCTGACATAG AAGGTGGGGA CCCTGCTAAT 
GCCAAGGAAT GCCAGTCACC AGACATATCC AGTAAGAATT GTAGAAGTCC TAAACAGAGT 
AAGCCCTGTT CTCTGGTGCC TAATTTATCT CATGGAAAGA CGCCCTCAAA GCTAGATCCT 
GCCCAAGAAA CCATGGTGAA TCCATCAACA ACTGCTGATG TTAATTTTTG TAACACCGAT 
TCTCAGCAGA AACAGCTCCT TTCTAAGTCT CTCATTCCCC ATGCTCAGAA GCTACCTTCT 
ACACCTTTAA AGTTGCATTG TCCGTCATCT CCTCTTCTTG AACACCCTCC AAAATCTTCA 
ACACCAGTAC AACATGGTTA TCTTTCACCA AAGCCTCATT CACAAAAATT AGGTTCTCCA 
TATAGGCCAC ATCATCCCCA GCAGCATCAG GTGGGAACAC CACAAAGAGA GTCTCTTCCA 
AACTTTTACC CCGTAGTGCA AAGCCTTCAG CCTGCTAATC CACAGCCCTC TAGTGGCCAG 
TTGTTTTCCC AGACCCAGCC AGGACAGTGC TCCAATGTTT ATACTCCCTT TAATCAACAG 
ACCGCCAGCA GTCCTGCTCC ACCTCCCCCA CCCCCACCTC CAACAGCAAC TTACTATCAA 
AACCAGCAGC CCTCTATAAA CTTCCAGAAC TTTAGTCAGA TAAAGGGAGC TGTGTCCCAA 
CAAACTGTGT TTACACCTGG TCCAAATCAA GGTCATCCAA CTGCAGCAGG ACAACCAGCA 
ATTCAAGGAC ACCATGTAAA CTCTGGACAT TATTTGCCTT CTCAAAGCCC TAATGTACAT 
CTCCAAGCTA CAGGGGTTCC ACCACCCCCA CCGCCACCTC TTCCAAGCTG CCATCAGCAG 
CACTCTGTTC AGCATGTTAC AAGCCCAGGT CATACGGTTC CAGTAGCCAG TAGCTCACAT 
ATTCATTCTC AGACACCAGG GCATCATTTA CCTCCACCAC CCCCTCCACC ACCTGGCCCA 
AGTCCTCATC ATTCGCATCT TCAGCATGTA TCAGCAGGAC ACCAAGGCCT GCAAGTGCAG 
CACCAGCATG TTTTAAATAC AGTTCCTCCA CCTCCCCCAC CACCACCTCC ACCCCCTCCA 
CAAGCAGCAA ATGTTTTAGG TGGTGCTGGA AATCACACGG CATCAGCACA AGTTATTCAT 
ATGCAAACTC ATCAAGGACC TGTTTTCTCT TCAGGAGCTC ATCCTAGTGT AGCACCATAT 
TCAACACAAG CTTCCCATCA TAACCAAGTT GGACCTGTAC CACAGCACCA GTCTTCTGCC 
ACAGGACCGC ATCTCCCACT TCCTGGCCAG AACTCTCATA TTCAGACACA AGGACCAAAC 
AGTATTGCAA CACCTGCAGG TTTTTGTCCG CATCCTGGCT CTGTAAGCCT TGCTCATGGA 
GTTCAAGGAC CTCAGCAGGC ATCAACAGTG CCTGGCCAGG TTCCTATTCA TAGGCCACAG 
GTACCTCCGA CCTTCCAGAA TAATTACCAT GGCTCAGGAT GGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947810.1
CDS	722..5989
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCRALDKERAVLLQQKKREHLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_018092321.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092321.1

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGGTGACACC 
AGTGCAACAG AAAGTTGTGA TGAAGTTCCC ATGGAACTGT ACACTGCTTT CCAGCACACC 
CCAACAACTC TTACTCTCAC TGCCACTAGA GTAAATAAGG TTAATGACAA AAAGAGGAAG 
AAAAGTGGTG AGAAGGAGCA GAATAATTCT AAAGTTAAGA AGGCTTTTCG TGAAGGATCA 
AGAAAATCAT CACGTGTTAA GGGCTCTGCT CCAGAAATTG ATCCCTTAGA CAATTATGCT 
TTTGGATGGG AGAATAAAAT CAAGACTTGG ATGGACCACT ATGAAGAAGC AAGCAACAAC 
CAATACAGTG AAGGGGTTCA AAAAGAGGCA CAAAGGATTG CTTTGAGATT CAGTAACGAA 
AATGATAATA AAGAACAAAT AAAGACCAAC TTTAACACCA GCAATCTAAT TTTCAAGCCA 
CCGGTAGAGA GCTATCTACA AAAGAACAAA AAAATCTTAA AGGCTGCCAA AGACTTGCTT 
CCTGAAACAC TAATTATAGA ATACAGGGGA AAATTTATGC TCCGGGAGCA GTTTGAAGCA 
AATGGGTATT TCTTTAAAAG GCCATACCCT TTTATTTTGT TTTATTCTAA ATTTCATGGA 
TTAGAAATGT GTGTTGATGC AAGGACATTT GGAAATGAGG CTCGATTTAT ACGACGTTCC 
TGCACACCTA ATGCTGAGGT GCGACATGTT ATTGAAGAAG GAACTATTCA CCTATATATA 
TACTCCATTT CAAGCATTCC AAAAGGGGCA GAAATCACTA TTGCTTTTGA TTTTGATTAT 
GCAAATTGTA AATATAAGGT GGATTGTGCA TGCTTCAAAG ACAATCCAGA CTGCCCAGTT 
CTCCGATGCA GTACAGAGCC TACAGAAAAT AACAGTGGCT ATGAAACTAG AAGAAAAAAG 
GGGAAAAAGG AAAAAGAACT TTTAAAAGAA AGAGAATCTC AAAACCAGAA CATTGTAATA 
GATTCAGATG GAGCAATTAA CAAATTTAAA ACATCAGATA ACAAGCAAAG AAAGCTCTCC 
CCTCTTAGAT TGTCAATATC AAACAACCAG GAGCCAGATA TTAATGATGA TATAGAAGAA 
AATACTCCTC TTAGCAATGA CGTAGAAATG GAAACAGAGG AGCAGATTGC AGAAAGAAAA 
AGGAAGATGA CTAGAGAAGA AAGAAAAATG GAAGCTATTC TGCAAGCTTT TGCAAGACTG 
GAAAAAAGAG AAAAAAGAAG AGAACAAGCT TTAGAAAGAA TTGGCACTTC CAAACCAGAC 
GTGAAAACAG AGAGCAAGGA CTTACAGATT ATCAGTGAAT TGGAGCTCCC TCCGGAACCA 
GTTAAGGAAG AACCTGCCAG CAAACCTACT CCTGCCAAAA TAAATCGCAC AAAGCAGCGA 
AAGAGTTTTA CAAGAAGTAG GACTCATATT GGACAACAGC GTCGTCGGCA CCGGACTATA 
AGTACCTGCT CAGATATCCA GCCATCTTCC CCAGATGTTG AGGTTGTACC ACAGCAGATT 
GAAACAGAAA ATAATGCTGT ACCTGAAGAA CCTCCAAATG AAAGCAGTCC CCATAATACA 
GAAGAAGTTC CAGAGACAGA ATCTTGTGTT CAAAGCAAAT GTTCTATAAA GTACCCAAAA 
ACAAAAAAGC ACTTGGTAAA TGAATGGTTA AATGAAAAGC ACGAGAAAGC TGGCAAACCT 
GGAGAGCCCA TATTGGAAAG ACCTTTAAGA ATAACCACAG ATCCTGAAGT CCTTGCTACT 
CAGCTGAATT CCTTACCAGG TTTAATGTAT AGTCCTCATG TGTACACCAC TCCTAAGCAT 
TATATTAGGT TTACATCACC ATTCCTTTCT GAGAAAAAGA AGAAGAAAAA TGAAGAGAAT 
GTTTCCAGTT GTTGCAAAAA GCGTTGGTTG AAGAAAGCTC TGGAAGAAGA AAATTCTTCG 
GGGGTTAATC CATTTGGTTC TCCTGCTCAG GACCGATCTC AAAGTCCGGT TTCATTACAT 
GAAAATAAAT GCCATCTAAT AATGAATGGC AGCTCTCCCC TGGCAGATTT TACAACTCCA 
CTGAAGAAAC GAAGGTTGTT TAATTTTTTG GATTGCAATT TTTCCGAAAG TTCAACACCA 
GTTGCATCTC CTTATGCTAC CCCTACACAT ACTGATATTA CTTCCATGGA TACATCCTTA 
TTTACTACTC CACCCAGGGT AAAAGTAGAA GAGGAAATCT GTCGCAACGG TTGCAAGCAA 
ACCTACTCAC CAGTTACTCC AGTAACACCC TGTCTGCGTG GAAACAACAT GCATTTTGAG 
AGTATTTCTT CACCTGATAG TTCACCAGAA GTGAACCGAA GATCTTTTTG TCAGGAAGGA 
AATGACCGGG CATCTACAGC ATTGAATTCT TTCAGAAATA CCAGTTTGTC TGACATGGGG 
CTTCAAGAAA TAAAGACTTT TGGCTACTGC AGCCCTAGGA ATAGGACTGA TGTTTCTGGT 
CAGTGTCCTG GTGAAAATGA TGCTGCATCA GAGGTCCATT TTGGAGCAGA CATAAATGAA 
CATAGCTCTC TGCAAAAGGT CATGGAGCCT CCACAGGATA GGACTGATTT AAATAGCCTA 
CAGGAAACTG CTCTTAGTGG ACAAGGCACC ATGTATTCAA CTTGGATTAA AAGTCCAAAT 
AGAACAGGGG TGAACTTCTT GTCCATGAAT TCTAATTTAA GAGATTTAAC CCCTTCTCAC 
CAGCTTGAAG TTGGTGGTGG ATTCAGAATG AATGAATCCA AATGCTTGAT CCCAGAAGAC 
TCACGGGGCG TTTTCATAGA TGGGCCTATC TTCTGCTCTG CAGAGGAGGG AACTGTTTCT 
GGATTTGGAA GATCTCTTGG TGGTGATGGG ATATTAGACG GAAATGGTAC ACCACTTAAT 
CCTCCGCAAA AGAAAAAGGT GTCATTGCTG GAATATAGAA AAAGACAGAA AGAAGCAAGA 
AAAAGTGGCT CTAAACAGGA AAATTTATCT CTGGTCACTT TATCTCCTCA TCCGGATGGT 
ATTGTCAACT GCACCCCTTC TGAAAGTTGT AGTGCTGTTG CAGAGTGTAT TGATCAAAAT 
GACGCTAGCA CTTGTTTAAC TCAGCCATCT GAAGTCCAGT CTGTCCCTTC TGAAGAAACC 
GAACAAACTT CTTCAGTAAC TGACACCTCA TCCACTGAAA AAAATGATCC TGAAGTGCAG 
TGGACTGCAT CAACATGTGT TGAGCAAGTC AGAGAGAGAA GCTATCAAAG AGCTTTGTTA 
CTGAGTGACC ACAGAAAGGA CAAAGATGAT GCTGACATAG AAGGTGGGGA CCCTGCTAAT 
GCCAAGGAAT GCCAGTCACC AGACATATCC AGTAAGAATT GTAGAAGTCC TAAACAGAGT 
AAGCCCTGTT CTCTGGTGCC TAATTTATCT CATGGAAAGA CGCCCTCAAA GCTAGATCCT 
GCCCAAGAAA CCATGGTGAA TCCATCAACA ACTGCTGATG TTAATTTTTG TAACACCGAT 
TCTCAGCAGA AACAGCTCCT TTCTAAGTCT CTCATTCCCC ATGCTCAGAA GCTACCTTCT 
ACACCTTTAA AGTTGCATTG TCCGTCATCT CCTCTTCTTG AACACCCTCC AAAATCTTCA 
ACACCAGTAC AACATGGTTA TCTTTCACCA AAGCCTCATT CACAAAAATT AGGTTCTCCA 
TATAGGCCAC ATCATCCCCA GCAGCATCAG GTGGGAACAC CACAAAGAGA GTCTCTTCCA 
AACTTTTACC CCGTAGTGCA AAGCCTTCAG CCTGCTAATC CACAGCCCTC TAGTGGCCAG 
TTGTTTTCCC AGACCCAGCC AGGACAGTGC TCCAATGTTT ATACTCCCTT TAATCAACAG 
ACCGCCAGCA GTCCTGCTCC ACCTCCCCCA CCCCCACCTC CAACAGCAAC TTACTATCAA 
AACCAGCAGC CCTCTATAAA CTTCCAGAAC TTTAGTCAGA TAAAGGGAGC TGTGTCCCAA 
CAAACTGTGT TTACACCTGG TCCAAATCAA GGTCATCCAA CTGCAGCAGG ACAACCAGCA 
ATTCAAGGAC ACCATGTAAA CTCTGGACAT TATTTGCCTT CTCAAAGCCC TAATGTACAT 
CTCCAAGCTA CAGGGGTTCC ACCACCCCCA CCGCCACCTC TTCCAAGCTG CCATCAGCAG 
CACTCTGTTC AGCATGTTAC AAGCCCAGGT CATACGGTTC CAGTAGCCAG TAGCTCACAT 
ATTCATTCTC AGACACCAGG GCATCATTTA CCTCCACCAC CCCCTCCACC ACCTGGCCCA 
AGTCCTCATC ATTCGCATCT TCAGCATGTA TCAGCAGGAC ACCAAGGCCT GCAAGTGCAG 
CACCAGCATG TTTTAAATAC AGTTCCTCCA CCTCCCCCAC CACCACCTCC ACCCCCTCCA 
CAAGCAGCAA ATGTTTTAGG TGGTGCTGGA AATCACACGG CATCAGCACA AGTTATTCAT 
ATGCAAACTC ATCAAGGACC TGTTTTCTCT TCAGGAGCTC ATCCTAGTGT AGCACCATAT 
TCAACACAAG CTTCCCATCA TAACCAAGTT GGACCTGTAC CACAGCACCA GTCTTCTGCC 
ACAGGACCGC ATCTCCCACT TCCTGGCCAG AACTCTCATA TTCAGACACA AGGACCAAAC 
AGTATTGCAA CACCTGCAGG TTTTTGTCCG CATCCTGGCT CTGTAAGCCT TGCTCATGGA 
GTTCAAGGAC CTCAGCAGGC ATCAACAGTG CCTGGCCAGG TTCCTATTCA TAGGCCACAG 
GTACCTCCGA CCTTCCAGAA TAATTACCAT GGCTCAGGAT GGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_004913017.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913074.2 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54848
Clone ID Related Accession (Same CDS sequence)	XM_004913017.4
Accession Version	XM_004913017.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913017.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGATGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCCCCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913074.2
CDS	2078..7480
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSGTTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_004913017.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913017.4

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGATGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCCCCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_018092320.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947809.2 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	OXa54849
Clone ID Related Accession (Same CDS sequence)	XM_018092320.2
Accession Version	XM_018092320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive histone-lysine N-methyltransferase 2E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGGTGACAC CAGTGCAACA GAAAGTTGTG ATGAAGTTCC CATGGAACTG 
TACACTGCTT TCCAGCACAC CCCAACAACT CTTACTCTCA CTGCCACTAG AGTAAATAAG 
GTTAATGACA AAAAGAGGAA GAAAAGTGGT GAGAAGGAGC AGAATAATTC TAAAGTTAAG 
AAGGCTTTTC GTGAAGGATC AAGAAAATCA TCACGTGTTA AGGGCTCTGC TCCAGAAATT 
GATCCCTTAG ACAATTATGC TTTTGGATGG GAGAATAAAA TCAAGACTTG GATGGACCAC 
TATGAAGAAG CAAGCAACAA CCAATACAGT GAAGGGGTTC AAAAAGAGGC ACAAAGGATT 
GCTTTGAGAT TCAGTAACGA AAATGATAAT AAAGAACAAA TAAAGACCAA CTTTAACACC 
AGCAATCTAA TTTTCAAGCC ACCGGTAGAG AGCTATCTAC AAAAGAACAA AAAAATCTTA 
AAGGCTGCCA AAGACTTGCT TCCTGAAACA CTAATTATAG AATACAGGGG AAAATTTATG 
CTCCGGGAGC AGTTTGAAGC AAATGGGTAT TTCTTTAAAA GGCCATACCC TTTTATTTTG 
TTTTATTCTA AATTTCATGG ATTAGAAATG TGTGTTGATG CAAGGACATT TGGAAATGAG 
GCTCGATTTA TACGACGTTC CTGCACACCT AATGCTGAGG TGCGACATGT TATTGAAGAA 
GGAACTATTC ACCTATATAT ATACTCCATT TCAAGCATTC CAAAAGGGGC AGAAATCACT 
ATTGCTTTTG ATTTTGATTA TGCAAATTGT AAATATAAGG TGGATTGTGC ATGCTTCAAA 
GACAATCCAG ACTGCCCAGT TCTCCGATGC AGTACAGAGC CTACAGAAAA TAACAGTGGC 
TATGAAACTA GAAGAAAAAA GGGGAAAAAG GAAAAAGAAC TTTTAAAAGA AAGAGAATCT 
CAAAACCAGA ACATTGTAAT AGATTCAGAT GGAGCAATTA ACAAATTTAA AACATCAGAT 
AACAAGCAAA GAAAGCTCTC CCCTCTTAGA TTGTCAATAT CAAACAACCA GGAGCCAGAT 
ATTAATGATG ATATAGAAGA AAATACTCCT CTTAGCAATG ACGTAGAAAT GGAAACAGAG 
GAGCAGATTG CAGAAAGAAA AAGGAAGATG ACTAGAGAAG AAAGAAAAAT GGAAGCTATT 
CTGCAAGCTT TTGCAAGACT GGAAAAAAGA GAAAAAAGAA GAGAACAAGC TTTAGAAAGA 
ATTGGCACTT CCAAACCAGA TGTGAAAACA GAGAGCAAGG ACTTACAGAT TATCAGTGAA 
TTGGAGCTCC CTCCGGAACC AGTTAAGGAA GAACCTGCCA GCAAACCTAC TCCTGCCAAA 
ATAAATCGCA CAAAGCAGCG AAAGAGTTTT ACAAGAAGTA GGACTCATAT TGGACAACAG 
CGTCGTCGGC ACCGGACTAT AAGTACCTGC TCAGATATCC AGCCATCTTC CCCAGATGTT 
GAGGTTGTAC CACAGCAGAT TGAAACAGAA AATAATGCTG TACCTGAAGA ACCTCCAAAT 
GAAAGCAGTC CCCATAATAC AGAAGAAGTT CCAGAGACAG AATCTTGTGT TCAAAGCAAA 
TGTTCTATAA AGTACCCAAA AACAAAAAAG CACTTGGTAA ATGAATGGTT AAATGAAAAG 
CACGAGAAAG CTGGCAAACC TGGAGAGCCC ATATTGGAAA GACCTTTAAG AATAACCACA 
GATCCTGAAG TCCTTGCTAC TCAGCTGAAT TCCTTACCAG GTTTAATGTA TAGTCCTCAT 
GTGTACACCA CTCCTAAGCA TTATATTAGG TTTACATCAC CATTCCTTTC TGAGAAAAAG 
AAGAAGAAAA ATGAAGAGAA TGTTTCCAGT TGTTGCAAAA AGCGTTGGTT GAAGAAAGCT 
CTGGAAGAAG AAAATTCTTC GGGGGTTAAT CCATTTGGTT CTCCTGCTCA GGACCGATCT 
CAAAGTCCGG TTTCATTACA TGAAAATAAA TGCCATCTAA TAATGAATGG CAGCTCTCCC 
CTGGCAGATT TTACAACTCC ACTGAAGAAA CGAAGGTTGT TTAATTTTTT GGATTGCAAT 
TTTTCCGAAA GTTCAACACC AGTTGCATCT CCTTATGCTA CCCCTACACA TACTGATATT 
ACTTCCATGG ATACATCCTT ATTTACTACT CCACCCAGGG TAAAAGTAGA AGAGGAAATC 
TGTCGCAACG GTTGCAAGCA AACCTACTCA CCAGTTACTC CAGTAACACC CTGTCTGCGT 
GGAAACAACA TGCATTTTGA GAGTATTTCT TCACCTGATA GTTCACCAGA AGTGAACCGA 
AGATCTTTTT GTCAGGAAGG AAATGACCGG GCATCTACAG CATTGAATTC TTTCAGAAAT 
ACCAGTTTGT CTGACATGGG GCTTCAAGAA ATAAAGACTT TTGGCTACTG CAGCCCTAGG 
AATAGGACTG ATGTTTCTGG TCAGTGTCCT GGTGAAAATG ATGCTGCATC AGAGGTCCAT 
TTTGGAGCAG ACATAAATGA ACATAGCTCT CTGCAAAAGG TCATGGAGCC TCCACAGGAT 
AGGACTGATT TAAATAGCCT ACAGGAAACT GCTCTTAGTG GACAAGGCAC CATGTATTCA 
ACTTGGATTA AAAGTCCAAA TAGAACAGGG GTGAACTTCT TGTCCATGAA TTCTAATTTA 
AGAGATTTAA CCCCTTCTCA CCAGCTTGAA GTTGGTGGTG GATTCAGAAT GAATGAATCC 
AAATGCTTGA TCCCAGAAGA CTCACGGGGC GTTTTCATAG ATGGGCCTAT CTTCTGCTCT 
GCAGAGGAGG GAACTGTTTC TGGATTTGGA AGATCTCTTG GTGGTGATGG GATATTAGAC 
GGAAATGGTA CACCACTTAA TCCTCCGCAA AAGAAAAAGG TGTCATTGCT GGAATATAGA 
AAAAGACAGA AAGAAGCAAG AAAAAGTGGC TCTAAACAGG AAAATTTATC TCTGGTCACT 
TTATCTCCTC ATCCGGATGG TATTGTCAAC TGCACCCCTT CTGAAAGTTG TAGTGCTGTT 
GCAGAGTGTA TTGATCAAAA TGACGCTAGC ACTTGTTTAA CTCAGCCATC TGAAGTCCAG 
TCTGTCCCTT CTGAAGAAAC CGAACAAACT TCTTCAGTAA CTGACACCTC ATCCACTGAA 
AAAAATGATC CTGAAGTGCA GTGGACTGCA TCAACATGTG TTGAGCAAGT CAGAGAGAGA 
AGCTATCAAA GAGCTTTGTT ACTGAGTGAC CACAGAAAGG ACAAAGATGA TGCTGACATA 
GAAGGTGGGG ACCCTGCTAA TGCCAAGGAA TGCCAGTCAC CAGACATATC CAGTAAGAAT 
TGTAGAAGTC CTAAACAGAG TAAGCCCTGT TCTCTGGTGC CTAATTTATC TCATGGAAAG 
ACGCCCTCAA AGCTAGATCC TGCCCAAGAA ACCATGGTGA ATCCATCAAC AACTGCTGAT 
GTTAATTTTT GTAACACCGA TTCTCAGCAG AAACAGCTCC TTTCTAAGTC TCTCATTCCC 
CATGCTCAGA AGCTACCTTC TACACCTTTA AAGTTGCATT GTCCGTCATC TCCTCTTCTT 
GAACACCCTC CAAAATCTTC AACACCAGTA CAACATGGTT ATCTTTCACC AAAGCCTCAT 
TCACAAAAAT TAGGTTCTCC ATATAGGCCA CATCATCCCC AGCAGCATCA GGTGGGAACA 
CCACAAAGAG AGTCTCTTCC AAACTTTTAC CCCGTAGTGC AAAGCCTTCA GCCTGCTAAT 
CCACAGCCCT CTAGTGGCCA GTTGTTTTCC CAGACCCAGC CAGGACAGTG CTCCAATGTT 
TATACTCCCT TTAATCAACA GACCGCCAGC AGTCCTGCTC CACCTCCCCC ACCCCCACCT 
CCAACAGCAA CTTACTATCA AAACCAGCAG CCCTCTATAA ACTTCCAGAA CTTTAGTCAG 
ATAAAGGGAG CTGTGTCCCA ACAAACTGTG TTTACACCTG GTCCAAATCA AGGTCATCCA 
ACTGCAGCAG GACAACCAGC AATTCAAGGA CACCATGTAA ACTCTGGACA TTATTTGCCT 
TCTCAAAGCC CTAATGTACA TCTCCAAGCT ACAGGGGTTC CACCACCCCC CCCGCCACCT 
CTTCCAAGCT GCCATCAGCA GCACTCTGTT CAGCATGTTA CAAGCCCAGG TCATACGGTT 
CCAGTAGCCA GTAGCTCACA TATTCATTCT CAGACACCAG GGCATCATTT ACCTCCACCA 
CCCCCTCCAC CACCTGGCCC AAGTCCTCAT CATTCGCATC TTCAGCATGT ATCAGCAGGA 
CACCAAGGCC TGCAAGTGCA GCACCAGCAT GTTTTAAATA CAGTTCCTCC ACCTCCCCCA 
CCACCACCTC CACCCCCTCC ACAAGCAGCA AATGTTTTAG GTGGTGCTGG AAATCACACG 
GCATCAGCAC AAGTTATTCA TATGCAAACT CATCAAGGAC CTGTTTTCTC TTCAGGAGCT 
CATCCTAGTG TAGCACCATA TTCAACACAA GCTTCCCATC ATAACCAAGT TGGACCTGTA 
CCACAGCACC AGTCTTCTGC CACAGGACCG CATCTCCCAC TTCCTGGCCA GAACTCTCAT 
ATTCAGACAC AAGGACCAAA CAGTATTGCA ACACCTGCAG GTTTTTGTCC GCATCCTGGC 
TCTGTAAGCC TTGCTCATGG AGTTCAAGGA CCTCAGCAGG CATCAACAGT GCCTGGCCAG 
GTTCCTATTC ATAGGCCACA GGTACCTCCG ACCTTCCAGA ATAATTACCA TGGCTCAGGA 
TGGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947809.2
CDS	2078..7426
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSGTTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_018092320.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092320.2

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGGTGACAC CAGTGCAACA GAAAGTTGTG ATGAAGTTCC CATGGAACTG 
TACACTGCTT TCCAGCACAC CCCAACAACT CTTACTCTCA CTGCCACTAG AGTAAATAAG 
GTTAATGACA AAAAGAGGAA GAAAAGTGGT GAGAAGGAGC AGAATAATTC TAAAGTTAAG 
AAGGCTTTTC GTGAAGGATC AAGAAAATCA TCACGTGTTA AGGGCTCTGC TCCAGAAATT 
GATCCCTTAG ACAATTATGC TTTTGGATGG GAGAATAAAA TCAAGACTTG GATGGACCAC 
TATGAAGAAG CAAGCAACAA CCAATACAGT GAAGGGGTTC AAAAAGAGGC ACAAAGGATT 
GCTTTGAGAT TCAGTAACGA AAATGATAAT AAAGAACAAA TAAAGACCAA CTTTAACACC 
AGCAATCTAA TTTTCAAGCC ACCGGTAGAG AGCTATCTAC AAAAGAACAA AAAAATCTTA 
AAGGCTGCCA AAGACTTGCT TCCTGAAACA CTAATTATAG AATACAGGGG AAAATTTATG 
CTCCGGGAGC AGTTTGAAGC AAATGGGTAT TTCTTTAAAA GGCCATACCC TTTTATTTTG 
TTTTATTCTA AATTTCATGG ATTAGAAATG TGTGTTGATG CAAGGACATT TGGAAATGAG 
GCTCGATTTA TACGACGTTC CTGCACACCT AATGCTGAGG TGCGACATGT TATTGAAGAA 
GGAACTATTC ACCTATATAT ATACTCCATT TCAAGCATTC CAAAAGGGGC AGAAATCACT 
ATTGCTTTTG ATTTTGATTA TGCAAATTGT AAATATAAGG TGGATTGTGC ATGCTTCAAA 
GACAATCCAG ACTGCCCAGT TCTCCGATGC AGTACAGAGC CTACAGAAAA TAACAGTGGC 
TATGAAACTA GAAGAAAAAA GGGGAAAAAG GAAAAAGAAC TTTTAAAAGA AAGAGAATCT 
CAAAACCAGA ACATTGTAAT AGATTCAGAT GGAGCAATTA ACAAATTTAA AACATCAGAT 
AACAAGCAAA GAAAGCTCTC CCCTCTTAGA TTGTCAATAT CAAACAACCA GGAGCCAGAT 
ATTAATGATG ATATAGAAGA AAATACTCCT CTTAGCAATG ACGTAGAAAT GGAAACAGAG 
GAGCAGATTG CAGAAAGAAA AAGGAAGATG ACTAGAGAAG AAAGAAAAAT GGAAGCTATT 
CTGCAAGCTT TTGCAAGACT GGAAAAAAGA GAAAAAAGAA GAGAACAAGC TTTAGAAAGA 
ATTGGCACTT CCAAACCAGA TGTGAAAACA GAGAGCAAGG ACTTACAGAT TATCAGTGAA 
TTGGAGCTCC CTCCGGAACC AGTTAAGGAA GAACCTGCCA GCAAACCTAC TCCTGCCAAA 
ATAAATCGCA CAAAGCAGCG AAAGAGTTTT ACAAGAAGTA GGACTCATAT TGGACAACAG 
CGTCGTCGGC ACCGGACTAT AAGTACCTGC TCAGATATCC AGCCATCTTC CCCAGATGTT 
GAGGTTGTAC CACAGCAGAT TGAAACAGAA AATAATGCTG TACCTGAAGA ACCTCCAAAT 
GAAAGCAGTC CCCATAATAC AGAAGAAGTT CCAGAGACAG AATCTTGTGT TCAAAGCAAA 
TGTTCTATAA AGTACCCAAA AACAAAAAAG CACTTGGTAA ATGAATGGTT AAATGAAAAG 
CACGAGAAAG CTGGCAAACC TGGAGAGCCC ATATTGGAAA GACCTTTAAG AATAACCACA 
GATCCTGAAG TCCTTGCTAC TCAGCTGAAT TCCTTACCAG GTTTAATGTA TAGTCCTCAT 
GTGTACACCA CTCCTAAGCA TTATATTAGG TTTACATCAC CATTCCTTTC TGAGAAAAAG 
AAGAAGAAAA ATGAAGAGAA TGTTTCCAGT TGTTGCAAAA AGCGTTGGTT GAAGAAAGCT 
CTGGAAGAAG AAAATTCTTC GGGGGTTAAT CCATTTGGTT CTCCTGCTCA GGACCGATCT 
CAAAGTCCGG TTTCATTACA TGAAAATAAA TGCCATCTAA TAATGAATGG CAGCTCTCCC 
CTGGCAGATT TTACAACTCC ACTGAAGAAA CGAAGGTTGT TTAATTTTTT GGATTGCAAT 
TTTTCCGAAA GTTCAACACC AGTTGCATCT CCTTATGCTA CCCCTACACA TACTGATATT 
ACTTCCATGG ATACATCCTT ATTTACTACT CCACCCAGGG TAAAAGTAGA AGAGGAAATC 
TGTCGCAACG GTTGCAAGCA AACCTACTCA CCAGTTACTC CAGTAACACC CTGTCTGCGT 
GGAAACAACA TGCATTTTGA GAGTATTTCT TCACCTGATA GTTCACCAGA AGTGAACCGA 
AGATCTTTTT GTCAGGAAGG AAATGACCGG GCATCTACAG CATTGAATTC TTTCAGAAAT 
ACCAGTTTGT CTGACATGGG GCTTCAAGAA ATAAAGACTT TTGGCTACTG CAGCCCTAGG 
AATAGGACTG ATGTTTCTGG TCAGTGTCCT GGTGAAAATG ATGCTGCATC AGAGGTCCAT 
TTTGGAGCAG ACATAAATGA ACATAGCTCT CTGCAAAAGG TCATGGAGCC TCCACAGGAT 
AGGACTGATT TAAATAGCCT ACAGGAAACT GCTCTTAGTG GACAAGGCAC CATGTATTCA 
ACTTGGATTA AAAGTCCAAA TAGAACAGGG GTGAACTTCT TGTCCATGAA TTCTAATTTA 
AGAGATTTAA CCCCTTCTCA CCAGCTTGAA GTTGGTGGTG GATTCAGAAT GAATGAATCC 
AAATGCTTGA TCCCAGAAGA CTCACGGGGC GTTTTCATAG ATGGGCCTAT CTTCTGCTCT 
GCAGAGGAGG GAACTGTTTC TGGATTTGGA AGATCTCTTG GTGGTGATGG GATATTAGAC 
GGAAATGGTA CACCACTTAA TCCTCCGCAA AAGAAAAAGG TGTCATTGCT GGAATATAGA 
AAAAGACAGA AAGAAGCAAG AAAAAGTGGC TCTAAACAGG AAAATTTATC TCTGGTCACT 
TTATCTCCTC ATCCGGATGG TATTGTCAAC TGCACCCCTT CTGAAAGTTG TAGTGCTGTT 
GCAGAGTGTA TTGATCAAAA TGACGCTAGC ACTTGTTTAA CTCAGCCATC TGAAGTCCAG 
TCTGTCCCTT CTGAAGAAAC CGAACAAACT TCTTCAGTAA CTGACACCTC ATCCACTGAA 
AAAAATGATC CTGAAGTGCA GTGGACTGCA TCAACATGTG TTGAGCAAGT CAGAGAGAGA 
AGCTATCAAA GAGCTTTGTT ACTGAGTGAC CACAGAAAGG ACAAAGATGA TGCTGACATA 
GAAGGTGGGG ACCCTGCTAA TGCCAAGGAA TGCCAGTCAC CAGACATATC CAGTAAGAAT 
TGTAGAAGTC CTAAACAGAG TAAGCCCTGT TCTCTGGTGC CTAATTTATC TCATGGAAAG 
ACGCCCTCAA AGCTAGATCC TGCCCAAGAA ACCATGGTGA ATCCATCAAC AACTGCTGAT 
GTTAATTTTT GTAACACCGA TTCTCAGCAG AAACAGCTCC TTTCTAAGTC TCTCATTCCC 
CATGCTCAGA AGCTACCTTC TACACCTTTA AAGTTGCATT GTCCGTCATC TCCTCTTCTT 
GAACACCCTC CAAAATCTTC AACACCAGTA CAACATGGTT ATCTTTCACC AAAGCCTCAT 
TCACAAAAAT TAGGTTCTCC ATATAGGCCA CATCATCCCC AGCAGCATCA GGTGGGAACA 
CCACAAAGAG AGTCTCTTCC AAACTTTTAC CCCGTAGTGC AAAGCCTTCA GCCTGCTAAT 
CCACAGCCCT CTAGTGGCCA GTTGTTTTCC CAGACCCAGC CAGGACAGTG CTCCAATGTT 
TATACTCCCT TTAATCAACA GACCGCCAGC AGTCCTGCTC CACCTCCCCC ACCCCCACCT 
CCAACAGCAA CTTACTATCA AAACCAGCAG CCCTCTATAA ACTTCCAGAA CTTTAGTCAG 
ATAAAGGGAG CTGTGTCCCA ACAAACTGTG TTTACACCTG GTCCAAATCA AGGTCATCCA 
ACTGCAGCAG GACAACCAGC AATTCAAGGA CACCATGTAA ACTCTGGACA TTATTTGCCT 
TCTCAAAGCC CTAATGTACA TCTCCAAGCT ACAGGGGTTC CACCACCCCC CCCGCCACCT 
CTTCCAAGCT GCCATCAGCA GCACTCTGTT CAGCATGTTA CAAGCCCAGG TCATACGGTT 
CCAGTAGCCA GTAGCTCACA TATTCATTCT CAGACACCAG GGCATCATTT ACCTCCACCA 
CCCCCTCCAC CACCTGGCCC AAGTCCTCAT CATTCGCATC TTCAGCATGT ATCAGCAGGA 
CACCAAGGCC TGCAAGTGCA GCACCAGCAT GTTTTAAATA CAGTTCCTCC ACCTCCCCCA 
CCACCACCTC CACCCCCTCC ACAAGCAGCA AATGTTTTAG GTGGTGCTGG AAATCACACG 
GCATCAGCAC AAGTTATTCA TATGCAAACT CATCAAGGAC CTGTTTTCTC TTCAGGAGCT 
CATCCTAGTG TAGCACCATA TTCAACACAA GCTTCCCATC ATAACCAAGT TGGACCTGTA 
CCACAGCACC AGTCTTCTGC CACAGGACCG CATCTCCCAC TTCCTGGCCA GAACTCTCAT 
ATTCAGACAC AAGGACCAAA CAGTATTGCA ACACCTGCAG GTTTTTGTCC GCATCCTGGC 
TCTGTAAGCC TTGCTCATGG AGTTCAAGGA CCTCAGCAGG CATCAACAGT GCCTGGCCAG 
GTTCCTATTC ATAGGCCACA GGTACCTCCG ACCTTCCAGA ATAATTACCA TGGCTCAGGA 
TGGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_012960004.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815458.2 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

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CloneID	OXa54850
Clone ID Related Accession (Same CDS sequence)	XM_012960004.3
Accession Version	XM_012960004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGATCTTCAA 
ACCTGCTACA ATGGCAGAGG CAACAACGGA GGCCTTTTAT CTGATGGTGA CACCAGTGCA 
ACAGAAAGTT GTGATGAAGT TCCCATGGAA CTGTACACTG CTTTCCAGCA CACCCCAACA 
ACTCTTACTC TCACTGCCAC TAGAGTAAAT AAGGTTAATG ACAAAAAGAG GAAGAAAAGT 
GGTGAGAAGG AGCAGAATAA TTCTAAAGTT AAGAAGGCTT TTCGTGAAGG ATCAAGAAAA 
TCATCACGTG TTAAGGGCTC TGCTCCAGAA ATTGATCCCT TAGACAATTA TGCTTTTGGA 
TGGGAGAATA AAATCAAGAC TTGGATGGAC CACTATGAAG AAGCAAGCAA CAACCAATAC 
AGTGAAGGGG TTCAAAAAGA GGCACAAAGG ATTGCTTTGA GATTCAGTAA CGAAAATGAT 
AATAAAGAAC AAATAAAGAC CAACTTTAAC ACCAGCAATC TAATTTTCAA GCCACCGGTA 
GAGAGCTATC TACAAAAGAA CAAAAAAATC TTAAAGGCTG CCAAAGACTT GCTTCCTGAA 
ACACTAATTA TAGAATACAG GGGAAAATTT ATGCTCCGGG AGCAGTTTGA AGCAAATGGG 
TATTTCTTTA AAAGGCCATA CCCTTTTATT TTGTTTTATT CTAAATTTCA TGGATTAGAA 
ATGTGTGTTG ATGCAAGGAC ATTTGGAAAT GAGGCTCGAT TTATACGACG TTCCTGCACA 
CCTAATGCTG AGGTGCGACA TGTTATTGAA GAAGGAACTA TTCACCTATA TATATACTCC 
ATTTCAAGCA TTCCAAAAGG GGCAGAAATC ACTATTGCTT TTGATTTTGA TTATGCAAAT 
TGTAAATATA AGGTGGATTG TGCATGCTTC AAAGACAATC CAGACTGCCC AGTTCTCCGA 
TGCAGTACAG AGCCTACAGA AAATAACAGT GGCTATGAAA CTAGAAGAAA AAAGGGGAAA 
AAGGAAAAAG AACTTTTAAA AGAAAGAGAA TCTCAAAACC AGAACATTGT AATAGATTCA 
GATGGAGCAA TTAACAAATT TAAAACATCA GATAACAAGC AAAGAAAGCT CTCCCCTCTT 
AGATTGTCAA TATCAAACAA CCAGGAGCCA GATATTAATG ATGATATAGA AGAAAATACT 
CCTCTTAGCA ATGACGTAGA AATGGAAACA GAGGAGCAGA TTGCAGAAAG AAAAAGGAAG 
ATGACTAGAG AAGAAAGAAA AATGGAAGCT ATTCTGCAAG CTTTTGCAAG ACTGGAAAAA 
AGAGAAAAAA GAAGAGAACA AGCTTTAGAA AGAATTGGCA CTTCCAAACC AGATGTGAAA 
ACAGAGAGCA AGGACTTACA GATTATCAGT GAATTGGAGC TCCCTCCGGA ACCAGTTAAG 
GAAGAACCTG CCAGCAAACC TACTCCTGCC AAAATAAATC GCACAAAGCA GCGAAAGAGT 
TTTACAAGAA GTAGGACTCA TATTGGACAA CAGCGTCGTC GGCACCGGAC TATAAGTACC 
TGCTCAGATA TCCAGCCATC TTCCCCAGAT GTTGAGGTTG TACCACAGCA GATTGAAACA 
GAAAATAATG CTGTACCTGA AGAACCTCCA AATGAAAGCA GTCCCCATAA TACAGAAGAA 
GTTCCAGAGA CAGAATCTTG TGTTCAAAGC AAATGTTCTA TAAAGTACCC AAAAACAAAA 
AAGCACTTGG TAAATGAATG GTTAAATGAA AAGCACGAGA AAGCTGGCAA ACCTGGAGAG 
CCCATATTGG AAAGACCTTT AAGAATAACC ACAGATCCTG AAGTCCTTGC TACTCAGCTG 
AATTCCTTAC CAGGTTTAAT GTATAGTCCT CATGTGTACA CCACTCCTAA GCATTATATT 
AGGTTTACAT CACCATTCCT TTCTGAGAAA AAGAAGAAGA AAAATGAAGA GAATGTTTCC 
AGTTGTTGCA AAAAGCGTTG GTTGAAGAAA GCTCTGGAAG AAGAAAATTC TTCGGGGGTT 
AATCCATTTG GTTCTCCTGC TCAGGACCGA TCTCAAAGTC CGGTTTCATT ACATGAAAAT 
AAATGCCATC TAATAATGAA TGGCAGCTCT CCCCTGGCAG ATTTTACAAC TCCACTGAAG 
AAACGAAGGT TGTTTAATTT TTTGGATTGC AATTTTTCCG AAAGTTCAAC ACCAGTTGCA 
TCTCCTTATG CTACCCCTAC ACATACTGAT ATTACTTCCA TGGATACATC CTTATTTACT 
ACTCCACCCA GGGTAAAAGT AGAAGAGGAA ATCTGTCGCA ACGGTTGCAA GCAAACCTAC 
TCACCAGTTA CTCCAGTAAC ACCCTGTCTG CGTGGAAACA ACATGCATTT TGAGAGTATT 
TCTTCACCTG ATAGTTCACC AGAAGTGAAC CGAAGATCTT TTTGTCAGGA AGGAAATGAC 
CGGGCATCTA CAGCATTGAA TTCTTTCAGA AATACCAGTT TGTCTGACAT GGGGCTTCAA 
GAAATAAAGA CTTTTGGCTA CTGCAGCCCT AGGAATAGGA CTGATGTTTC TGGTCAGTGT 
CCTGGTGAAA ATGATGCTGC ATCAGAGGTC CATTTTGGAG CAGACATAAA TGAACATAGC 
TCTCTGCAAA AGGTCATGGA GCCTCCACAG GATAGGACTG ATTTAAATAG CCTACAGGAA 
ACTGCTCTTA GTGGACAAGG CACCATGTAT TCAACTTGGA TTAAAAGTCC AAATAGAACA 
GGGGTGAACT TCTTGTCCAT GAATTCTAAT TTAAGAGATT TAACCCCTTC TCACCAGCTT 
GAAGTTGGTG GTGGATTCAG AATGAATGAA TCCAAATGCT TGATCCCAGA AGACTCACGG 
GGCGTTTTCA TAGATGGGCC TATCTTCTGC TCTGCAGAGG AGGGAACTGT TTCTGGATTT 
GGAAGATCTC TTGGTGGTGA TGGGATATTA GACGGAAATG GTACACCACT TAATCCTCCG 
CAAAAGAAAA AGGTGTCATT GCTGGAATAT AGAAAAAGAC AGAAAGAAGC AAGAAAAAGT 
GGCTCTAAAC AGGAAAATTT ATCTCTGGTC ACTTTATCTC CTCATCCGGA TGGTATTGTC 
AACTGCACCC CTTCTGAAAG TTGTAGTGCT GTTGCAGAGT GTATTGATCA AAATGACGCT 
AGCACTTGTT TAACTCAGCC ATCTGAAGTC CAGTCTGTCC CTTCTGAAGA AACCGAACAA 
ACTTCTTCAG TAACTGACAC CTCATCCACT GAAAAAAATG ATCCTGAAGT GCAGTGGACT 
GCATCAACAT GTGTTGAGCA AGTCAGAGAG AGAAGCTATC AAAGAGCTTT GTTACTGAGT 
GACCACAGAA AGGACAAAGA TGATGCTGAC ATAGAAGGTG GGGACCCTGC TAATGCCAAG 
GAATGCCAGT CACCAGACAT ATCCAGTAAG AATTGTAGAA GTCCTAAACA GAGTAAGCCC 
TGTTCTCTGG TGCCTAATTT ATCTCATGGA AAGACGCCCT CAAAGCTAGA TCCTGCCCAA 
GAAACCATGG TGAATCCATC AACAACTGCT GATGTTAATT TTTGTAACAC CGATTCTCAG 
CAGAAACAGC TCCTTTCTAA GTCTCTCATT CCCCATGCTC AGAAGCTACC TTCTACACCT 
TTAAAGTTGC ATTGTCCGTC ATCTCCTCTT CTTGAACACC CTCCAAAATC TTCAACACCA 
GTACAACATG GTTATCTTTC ACCAAAGCCT CATTCACAAA AATTAGGTTC TCCATATAGG 
CCACATCATC CCCAGCAGCA TCAGGTGGGA ACACCACAAA GAGAGTCTCT TCCAAACTTT 
TACCCCGTAG TGCAAAGCCT TCAGCCTGCT AATCCACAGC CCTCTAGTGG CCAGTTGTTT 
TCCCAGACCC AGCCAGGACA GTGCTCCAAT GTTTATACTC CCTTTAATCA ACAGACCGCC 
AGCAGTCCTG CTCCACCTCC CCCACCCCCA CCTCCAACAG CAACTTACTA TCAAAACCAG 
CAGCCCTCTA TAAACTTCCA GAACTTTAGT CAGATAAAGG GAGCTGTGTC CCAACAAACT 
GTGTTTACAC CTGGTCCAAA TCAAGGTCAT CCAACTGCAG CAGGACAACC AGCAATTCAA 
GGACACCATG TAAACTCTGG ACATTATTTG CCTTCTCAAA GCCCTAATGT ACATCTCCAA 
GCTACAGGGG TTCCACCACC CCCCCCGCCA CCTCTTCCAA GCTGCCATCA GCAGCACTCT 
GTTCAGCATG TTACAAGCCC AGGTCATACG GTTCCAGTAG CCAGTAGCTC ACATATTCAT 
TCTCAGACAC CAGGGCATCA TTTACCTCCA CCACCCCCTC CACCACCTGG CCCAAGTCCT 
CATCATTCGC ATCTTCAGCA TGTATCAGCA GGACACCAAG GCCTGCAAGT GCAGCACCAG 
CATGTTTTAA ATACAGTTCC TCCACCTCCC CCACCACCAC CTCCACCCCC TCCACAAGCA 
GCAAATGTTT TAGGTGGTGC TGGAAATCAC ACGGCATCAG CACAAGTTAT TCATATGCAA 
ACTCATCAAG GACCTGTTTT CTCTTCAGGA GCTCATCCTA GTGTAGCACC ATATTCAACA 
CAAGCTTCCC ATCATAACCA AGTTGGACCT GTACCACAGC ACCAGTCTTC TGCCACAGGA 
CCGCATCTCC CACTTCCTGG CCAGAACTCT CATATTCAGA CACAAGGACC AAACAGTATT 
GCAACACCTG CAGGTTTTTG TCCGCATCCT GGCTCTGTAA GCCTTGCTCA TGGAGTTCAA 
GGACCTCAGC AGGCATCAAC AGTGCCTGGC CAGGTTCCTA TTCATAGGCC ACAGGTACCT 
CCGACCTTCC AGAATAATTA CCATGGCTCA GGATGGCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815458.2
CDS	2078..7399
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSGTTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_012960004.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960004.3

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGATCTTCAA 
ACCTGCTACA ATGGCAGAGG CAACAACGGA GGCCTTTTAT CTGATGGTGA CACCAGTGCA 
ACAGAAAGTT GTGATGAAGT TCCCATGGAA CTGTACACTG CTTTCCAGCA CACCCCAACA 
ACTCTTACTC TCACTGCCAC TAGAGTAAAT AAGGTTAATG ACAAAAAGAG GAAGAAAAGT 
GGTGAGAAGG AGCAGAATAA TTCTAAAGTT AAGAAGGCTT TTCGTGAAGG ATCAAGAAAA 
TCATCACGTG TTAAGGGCTC TGCTCCAGAA ATTGATCCCT TAGACAATTA TGCTTTTGGA 
TGGGAGAATA AAATCAAGAC TTGGATGGAC CACTATGAAG AAGCAAGCAA CAACCAATAC 
AGTGAAGGGG TTCAAAAAGA GGCACAAAGG ATTGCTTTGA GATTCAGTAA CGAAAATGAT 
AATAAAGAAC AAATAAAGAC CAACTTTAAC ACCAGCAATC TAATTTTCAA GCCACCGGTA 
GAGAGCTATC TACAAAAGAA CAAAAAAATC TTAAAGGCTG CCAAAGACTT GCTTCCTGAA 
ACACTAATTA TAGAATACAG GGGAAAATTT ATGCTCCGGG AGCAGTTTGA AGCAAATGGG 
TATTTCTTTA AAAGGCCATA CCCTTTTATT TTGTTTTATT CTAAATTTCA TGGATTAGAA 
ATGTGTGTTG ATGCAAGGAC ATTTGGAAAT GAGGCTCGAT TTATACGACG TTCCTGCACA 
CCTAATGCTG AGGTGCGACA TGTTATTGAA GAAGGAACTA TTCACCTATA TATATACTCC 
ATTTCAAGCA TTCCAAAAGG GGCAGAAATC ACTATTGCTT TTGATTTTGA TTATGCAAAT 
TGTAAATATA AGGTGGATTG TGCATGCTTC AAAGACAATC CAGACTGCCC AGTTCTCCGA 
TGCAGTACAG AGCCTACAGA AAATAACAGT GGCTATGAAA CTAGAAGAAA AAAGGGGAAA 
AAGGAAAAAG AACTTTTAAA AGAAAGAGAA TCTCAAAACC AGAACATTGT AATAGATTCA 
GATGGAGCAA TTAACAAATT TAAAACATCA GATAACAAGC AAAGAAAGCT CTCCCCTCTT 
AGATTGTCAA TATCAAACAA CCAGGAGCCA GATATTAATG ATGATATAGA AGAAAATACT 
CCTCTTAGCA ATGACGTAGA AATGGAAACA GAGGAGCAGA TTGCAGAAAG AAAAAGGAAG 
ATGACTAGAG AAGAAAGAAA AATGGAAGCT ATTCTGCAAG CTTTTGCAAG ACTGGAAAAA 
AGAGAAAAAA GAAGAGAACA AGCTTTAGAA AGAATTGGCA CTTCCAAACC AGATGTGAAA 
ACAGAGAGCA AGGACTTACA GATTATCAGT GAATTGGAGC TCCCTCCGGA ACCAGTTAAG 
GAAGAACCTG CCAGCAAACC TACTCCTGCC AAAATAAATC GCACAAAGCA GCGAAAGAGT 
TTTACAAGAA GTAGGACTCA TATTGGACAA CAGCGTCGTC GGCACCGGAC TATAAGTACC 
TGCTCAGATA TCCAGCCATC TTCCCCAGAT GTTGAGGTTG TACCACAGCA GATTGAAACA 
GAAAATAATG CTGTACCTGA AGAACCTCCA AATGAAAGCA GTCCCCATAA TACAGAAGAA 
GTTCCAGAGA CAGAATCTTG TGTTCAAAGC AAATGTTCTA TAAAGTACCC AAAAACAAAA 
AAGCACTTGG TAAATGAATG GTTAAATGAA AAGCACGAGA AAGCTGGCAA ACCTGGAGAG 
CCCATATTGG AAAGACCTTT AAGAATAACC ACAGATCCTG AAGTCCTTGC TACTCAGCTG 
AATTCCTTAC CAGGTTTAAT GTATAGTCCT CATGTGTACA CCACTCCTAA GCATTATATT 
AGGTTTACAT CACCATTCCT TTCTGAGAAA AAGAAGAAGA AAAATGAAGA GAATGTTTCC 
AGTTGTTGCA AAAAGCGTTG GTTGAAGAAA GCTCTGGAAG AAGAAAATTC TTCGGGGGTT 
AATCCATTTG GTTCTCCTGC TCAGGACCGA TCTCAAAGTC CGGTTTCATT ACATGAAAAT 
AAATGCCATC TAATAATGAA TGGCAGCTCT CCCCTGGCAG ATTTTACAAC TCCACTGAAG 
AAACGAAGGT TGTTTAATTT TTTGGATTGC AATTTTTCCG AAAGTTCAAC ACCAGTTGCA 
TCTCCTTATG CTACCCCTAC ACATACTGAT ATTACTTCCA TGGATACATC CTTATTTACT 
ACTCCACCCA GGGTAAAAGT AGAAGAGGAA ATCTGTCGCA ACGGTTGCAA GCAAACCTAC 
TCACCAGTTA CTCCAGTAAC ACCCTGTCTG CGTGGAAACA ACATGCATTT TGAGAGTATT 
TCTTCACCTG ATAGTTCACC AGAAGTGAAC CGAAGATCTT TTTGTCAGGA AGGAAATGAC 
CGGGCATCTA CAGCATTGAA TTCTTTCAGA AATACCAGTT TGTCTGACAT GGGGCTTCAA 
GAAATAAAGA CTTTTGGCTA CTGCAGCCCT AGGAATAGGA CTGATGTTTC TGGTCAGTGT 
CCTGGTGAAA ATGATGCTGC ATCAGAGGTC CATTTTGGAG CAGACATAAA TGAACATAGC 
TCTCTGCAAA AGGTCATGGA GCCTCCACAG GATAGGACTG ATTTAAATAG CCTACAGGAA 
ACTGCTCTTA GTGGACAAGG CACCATGTAT TCAACTTGGA TTAAAAGTCC AAATAGAACA 
GGGGTGAACT TCTTGTCCAT GAATTCTAAT TTAAGAGATT TAACCCCTTC TCACCAGCTT 
GAAGTTGGTG GTGGATTCAG AATGAATGAA TCCAAATGCT TGATCCCAGA AGACTCACGG 
GGCGTTTTCA TAGATGGGCC TATCTTCTGC TCTGCAGAGG AGGGAACTGT TTCTGGATTT 
GGAAGATCTC TTGGTGGTGA TGGGATATTA GACGGAAATG GTACACCACT TAATCCTCCG 
CAAAAGAAAA AGGTGTCATT GCTGGAATAT AGAAAAAGAC AGAAAGAAGC AAGAAAAAGT 
GGCTCTAAAC AGGAAAATTT ATCTCTGGTC ACTTTATCTC CTCATCCGGA TGGTATTGTC 
AACTGCACCC CTTCTGAAAG TTGTAGTGCT GTTGCAGAGT GTATTGATCA AAATGACGCT 
AGCACTTGTT TAACTCAGCC ATCTGAAGTC CAGTCTGTCC CTTCTGAAGA AACCGAACAA 
ACTTCTTCAG TAACTGACAC CTCATCCACT GAAAAAAATG ATCCTGAAGT GCAGTGGACT 
GCATCAACAT GTGTTGAGCA AGTCAGAGAG AGAAGCTATC AAAGAGCTTT GTTACTGAGT 
GACCACAGAA AGGACAAAGA TGATGCTGAC ATAGAAGGTG GGGACCCTGC TAATGCCAAG 
GAATGCCAGT CACCAGACAT ATCCAGTAAG AATTGTAGAA GTCCTAAACA GAGTAAGCCC 
TGTTCTCTGG TGCCTAATTT ATCTCATGGA AAGACGCCCT CAAAGCTAGA TCCTGCCCAA 
GAAACCATGG TGAATCCATC AACAACTGCT GATGTTAATT TTTGTAACAC CGATTCTCAG 
CAGAAACAGC TCCTTTCTAA GTCTCTCATT CCCCATGCTC AGAAGCTACC TTCTACACCT 
TTAAAGTTGC ATTGTCCGTC ATCTCCTCTT CTTGAACACC CTCCAAAATC TTCAACACCA 
GTACAACATG GTTATCTTTC ACCAAAGCCT CATTCACAAA AATTAGGTTC TCCATATAGG 
CCACATCATC CCCAGCAGCA TCAGGTGGGA ACACCACAAA GAGAGTCTCT TCCAAACTTT 
TACCCCGTAG TGCAAAGCCT TCAGCCTGCT AATCCACAGC CCTCTAGTGG CCAGTTGTTT 
TCCCAGACCC AGCCAGGACA GTGCTCCAAT GTTTATACTC CCTTTAATCA ACAGACCGCC 
AGCAGTCCTG CTCCACCTCC CCCACCCCCA CCTCCAACAG CAACTTACTA TCAAAACCAG 
CAGCCCTCTA TAAACTTCCA GAACTTTAGT CAGATAAAGG GAGCTGTGTC CCAACAAACT 
GTGTTTACAC CTGGTCCAAA TCAAGGTCAT CCAACTGCAG CAGGACAACC AGCAATTCAA 
GGACACCATG TAAACTCTGG ACATTATTTG CCTTCTCAAA GCCCTAATGT ACATCTCCAA 
GCTACAGGGG TTCCACCACC CCCCCCGCCA CCTCTTCCAA GCTGCCATCA GCAGCACTCT 
GTTCAGCATG TTACAAGCCC AGGTCATACG GTTCCAGTAG CCAGTAGCTC ACATATTCAT 
TCTCAGACAC CAGGGCATCA TTTACCTCCA CCACCCCCTC CACCACCTGG CCCAAGTCCT 
CATCATTCGC ATCTTCAGCA TGTATCAGCA GGACACCAAG GCCTGCAAGT GCAGCACCAG 
CATGTTTTAA ATACAGTTCC TCCACCTCCC CCACCACCAC CTCCACCCCC TCCACAAGCA 
GCAAATGTTT TAGGTGGTGC TGGAAATCAC ACGGCATCAG CACAAGTTAT TCATATGCAA 
ACTCATCAAG GACCTGTTTT CTCTTCAGGA GCTCATCCTA GTGTAGCACC ATATTCAACA 
CAAGCTTCCC ATCATAACCA AGTTGGACCT GTACCACAGC ACCAGTCTTC TGCCACAGGA 
CCGCATCTCC CACTTCCTGG CCAGAACTCT CATATTCAGA CACAAGGACC AAACAGTATT 
GCAACACCTG CAGGTTTTTG TCCGCATCCT GGCTCTGTAA GCCTTGCTCA TGGAGTTCAA 
GGACCTCAGC AGGCATCAAC AGTGCCTGGC CAGGTTCCTA TTCATAGGCC ACAGGTACCT 
CCGACCTTCC AGAATAATTA CCATGGCTCA GGATGGCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_004913019.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913076.2 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

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CloneID	OXa54851
Clone ID Related Accession (Same CDS sequence)	XM_004913019.4
Accession Version	XM_004913019.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGACTAG AGAAGAAAGA 
AAAATGGAAG CTATTCTGCA AGCTTTTGCA AGACTGGAAA AAAGAGAAAA AAGAAGAGAA 
CAAGCTTTAG AAAGAATTGG CACTTCCAAA CCAGATGTGA AAACAGAGAG CAAGGACTTA 
CAGATTATCA GTGAATTGGA GCTCCCTCCG GAACCAGTTA AGGAAGAACC TGCCAGCAAA 
CCTACTCCTG CCAAAATAAA TCGCACAAAG CAGCGAAAGA GTTTTACAAG AAGTAGGACT 
CATATTGGAC AACAGCGTCG TCGGCACCGG ACTATAAGTA CCTGCTCAGA TATCCAGCCA 
TCTTCCCCAG ATGTTGAGGT TGTACCACAG CAGATTGAAA CAGAAAATAA TGCTGTACCT 
GAAGAACCTC CAAATGAAAG CAGTCCCCAT AATACAGAAG AAGTTCCAGA GACAGAATCT 
TGTGTTCAAA GCAAATGTTC TATAAAGTAC CCAAAAACAA AAAAGCACTT GGTAAATGAA 
TGGTTAAATG AAAAGCACGA GAAAGCTGGC AAACCTGGAG AGCCCATATT GGAAAGACCT 
TTAAGAATAA CCACAGATCC TGAAGTCCTT GCTACTCAGC TGAATTCCTT ACCAGGTTTA 
ATGTATAGTC CTCATGTGTA CACCACTCCT AAGCATTATA TTAGGTTTAC ATCACCATTC 
CTTTCTGAGA AAAAGAAGAA GAAAAATGAA GAGAATGTTT CCAGTTGTTG CAAAAAGCGT 
TGGTTGAAGA AAGCTCTGGA AGAAGAAAAT TCTTCGGGGG TTAATCCATT TGGTTCTCCT 
GCTCAGGACC GATCTCAAAG TCCGGTTTCA TTACATGAAA ATAAATGCCA TCTAATAATG 
AATGGCAGCT CTCCCCTGGC AGATTTTACA ACTCCACTGA AGAAACGAAG GTTGTTTAAT 
TTTTTGGATT GCAATTTTTC CGAAAGTTCA ACACCAGTTG CATCTCCTTA TGCTACCCCT 
ACACATACTG ATATTACTTC CATGGATACA TCCTTATTTA CTACTCCACC CAGGGTAAAA 
GTAGAAGAGG AAATCTGTCG CAACGGTTGC AAGCAAACCT ACTCACCAGT TACTCCAGTA 
ACACCCTGTC TGCGTGGAAA CAACATGCAT TTTGAGAGTA TTTCTTCACC TGATAGTTCA 
CCAGAAGTGA ACCGAAGATC TTTTTGTCAG GAAGGAAATG ACCGGGCATC TACAGCATTG 
AATTCTTTCA GAAATACCAG TTTGTCTGAC ATGGGGCTTC AAGAAATAAA GACTTTTGGC 
TACTGCAGCC CTAGGAATAG GACTGATGTT TCTGGTCAGT GTCCTGGTGA AAATGATGCT 
GCATCAGAGG TCCATTTTGG AGCAGACATA AATGAACATA GCTCTCTGCA AAAGGTCATG 
GAGCCTCCAC AGGATAGGAC TGATTTAAAT AGCCTACAGG AAACTGCTCT TAGTGGACAA 
GGCACCATGT ATTCAACTTG GATTAAAAGT CCAAATAGAA CAGGGGTGAA CTTCTTGTCC 
ATGAATTCTA ATTTAAGAGA TTTAACCCCT TCTCACCAGC TTGAAGTTGG TGGTGGATTC 
AGAATGAATG AATCCAAATG CTTGATCCCA GAAGACTCAC GGGGCGTTTT CATAGATGGG 
CCTATCTTCT GCTCTGCAGA GGAGGGAACT GTTTCTGGAT TTGGAAGATC TCTTGGTGGT 
GATGGGATAT TAGACGGAAA TGGTACACCA CTTAATCCTC CGCAAAAGAA AAAGGTGTCA 
TTGCTGGAAT ATAGAAAAAG ACAGAAAGAA GCAAGAAAAA GTGGCTCTAA ACAGGAAAAT 
TTATCTCTGG TCACTTTATC TCCTCATCCG GATGGTATTG TCAACTGCAC CCCTTCTGAA 
AGTTGTAGTG CTGTTGCAGA GTGTATTGAT CAAAATGACG CTAGCACTTG TTTAACTCAG 
CCATCTGAAG TCCAGTCTGT CCCTTCTGAA GAAACCGAAC AAACTTCTTC AGTAACTGAC 
ACCTCATCCA CTGAAAAAAA TGATCCTGAA GTGCAGTGGA CTGCATCAAC ATGTGTTGAG 
CAAGTCAGAG AGAGAAGCTA TCAAAGAGCT TTGTTACTGA GTGACCACAG AAAGGACAAA 
GATGATGCTG ACATAGAAGG TGGGGACCCT GCTAATGCCA AGGAATGCCA GTCACCAGAC 
ATATCCAGTA AGAATTGTAG AAGTCCTAAA CAGAGTAAGC CCTGTTCTCT GGTGCCTAAT 
TTATCTCATG GAAAGACGCC CTCAAAGCTA GATCCTGCCC AAGAAACCAT GGTGAATCCA 
TCAACAACTG CTGATGTTAA TTTTTGTAAC ACCGATTCTC AGCAGAAACA GCTCCTTTCT 
AAGTCTCTCA TTCCCCATGC TCAGAAGCTA CCTTCTACAC CTTTAAAGTT GCATTGTCCG 
TCATCTCCTC TTCTTGAACA CCCTCCAAAA TCTTCAACAC CAGTACAACA TGGTTATCTT 
TCACCAAAGC CTCATTCACA AAAATTAGGT TCTCCATATA GGCCACATCA TCCCCAGCAG 
CATCAGGTGG GAACACCACA AAGAGAGTCT CTTCCAAACT TTTACCCCGT AGTGCAAAGC 
CTTCAGCCTG CTAATCCACA GCCCTCTAGT GGCCAGTTGT TTTCCCAGAC CCAGCCAGGA 
CAGTGCTCCA ATGTTTATAC TCCCTTTAAT CAACAGACCG CCAGCAGTCC TGCTCCACCT 
CCCCCACCCC CACCTCCAAC AGCAACTTAC TATCAAAACC AGCAGCCCTC TATAAACTTC 
CAGAACTTTA GTCAGATAAA GGGAGCTGTG TCCCAACAAA CTGTGTTTAC ACCTGGTCCA 
AATCAAGGTC ATCCAACTGC AGCAGGACAA CCAGCAATTC AAGGACACCA TGTAAACTCT 
GGACATTATT TGCCTTCTCA AAGCCCTAAT GTACATCTCC AAGCTACAGG GGTTCCACCA 
CCCCCCCCGC CACCTCTTCC AAGCTGCCAT CAGCAGCACT CTGTTCAGCA TGTTACAAGC 
CCAGGTCATA CGGTTCCAGT AGCCAGTAGC TCACATATTC ATTCTCAGAC ACCAGGGCAT 
CATTTACCTC CACCACCCCC TCCACCACCT GGCCCAAGTC CTCATCATTC GCATCTTCAG 
CATGTATCAG CAGGACACCA AGGCCTGCAA GTGCAGCACC AGCATGTTTT AAATACAGTT 
CCTCCACCTC CCCCACCACC ACCTCCACCC CCTCCACAAG CAGCAAATGT TTTAGGTGGT 
GCTGGAAATC ACACGGCATC AGCACAAGTT ATTCATATGC AAACTCATCA AGGACCTGTT 
TTCTCTTCAG GAGCTCATCC TAGTGTAGCA CCATATTCAA CACAAGCTTC CCATCATAAC 
CAAGTTGGAC CTGTACCACA GCACCAGTCT TCTGCCACAG GACCGCATCT CCCACTTCCT 
GGCCAGAACT CTCATATTCA GACACAAGGA CCAAACAGTA TTGCAACACC TGCAGGTTTT 
TGTCCGCATC CTGGCTCTGT AAGCCTTGCT CATGGAGTTC AAGGACCTCA GCAGGCATCA 
ACAGTGCCTG GCCAGGTTCC TATTCATAGG CCACAGGTAC CTCCGACCTT CCAGAATAAT 
TACCATGGCT CAGGATGGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913076.2
CDS	2078..7381
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSGTTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_004913019.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913019.4

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGACTAG AGAAGAAAGA 
AAAATGGAAG CTATTCTGCA AGCTTTTGCA AGACTGGAAA AAAGAGAAAA AAGAAGAGAA 
CAAGCTTTAG AAAGAATTGG CACTTCCAAA CCAGATGTGA AAACAGAGAG CAAGGACTTA 
CAGATTATCA GTGAATTGGA GCTCCCTCCG GAACCAGTTA AGGAAGAACC TGCCAGCAAA 
CCTACTCCTG CCAAAATAAA TCGCACAAAG CAGCGAAAGA GTTTTACAAG AAGTAGGACT 
CATATTGGAC AACAGCGTCG TCGGCACCGG ACTATAAGTA CCTGCTCAGA TATCCAGCCA 
TCTTCCCCAG ATGTTGAGGT TGTACCACAG CAGATTGAAA CAGAAAATAA TGCTGTACCT 
GAAGAACCTC CAAATGAAAG CAGTCCCCAT AATACAGAAG AAGTTCCAGA GACAGAATCT 
TGTGTTCAAA GCAAATGTTC TATAAAGTAC CCAAAAACAA AAAAGCACTT GGTAAATGAA 
TGGTTAAATG AAAAGCACGA GAAAGCTGGC AAACCTGGAG AGCCCATATT GGAAAGACCT 
TTAAGAATAA CCACAGATCC TGAAGTCCTT GCTACTCAGC TGAATTCCTT ACCAGGTTTA 
ATGTATAGTC CTCATGTGTA CACCACTCCT AAGCATTATA TTAGGTTTAC ATCACCATTC 
CTTTCTGAGA AAAAGAAGAA GAAAAATGAA GAGAATGTTT CCAGTTGTTG CAAAAAGCGT 
TGGTTGAAGA AAGCTCTGGA AGAAGAAAAT TCTTCGGGGG TTAATCCATT TGGTTCTCCT 
GCTCAGGACC GATCTCAAAG TCCGGTTTCA TTACATGAAA ATAAATGCCA TCTAATAATG 
AATGGCAGCT CTCCCCTGGC AGATTTTACA ACTCCACTGA AGAAACGAAG GTTGTTTAAT 
TTTTTGGATT GCAATTTTTC CGAAAGTTCA ACACCAGTTG CATCTCCTTA TGCTACCCCT 
ACACATACTG ATATTACTTC CATGGATACA TCCTTATTTA CTACTCCACC CAGGGTAAAA 
GTAGAAGAGG AAATCTGTCG CAACGGTTGC AAGCAAACCT ACTCACCAGT TACTCCAGTA 
ACACCCTGTC TGCGTGGAAA CAACATGCAT TTTGAGAGTA TTTCTTCACC TGATAGTTCA 
CCAGAAGTGA ACCGAAGATC TTTTTGTCAG GAAGGAAATG ACCGGGCATC TACAGCATTG 
AATTCTTTCA GAAATACCAG TTTGTCTGAC ATGGGGCTTC AAGAAATAAA GACTTTTGGC 
TACTGCAGCC CTAGGAATAG GACTGATGTT TCTGGTCAGT GTCCTGGTGA AAATGATGCT 
GCATCAGAGG TCCATTTTGG AGCAGACATA AATGAACATA GCTCTCTGCA AAAGGTCATG 
GAGCCTCCAC AGGATAGGAC TGATTTAAAT AGCCTACAGG AAACTGCTCT TAGTGGACAA 
GGCACCATGT ATTCAACTTG GATTAAAAGT CCAAATAGAA CAGGGGTGAA CTTCTTGTCC 
ATGAATTCTA ATTTAAGAGA TTTAACCCCT TCTCACCAGC TTGAAGTTGG TGGTGGATTC 
AGAATGAATG AATCCAAATG CTTGATCCCA GAAGACTCAC GGGGCGTTTT CATAGATGGG 
CCTATCTTCT GCTCTGCAGA GGAGGGAACT GTTTCTGGAT TTGGAAGATC TCTTGGTGGT 
GATGGGATAT TAGACGGAAA TGGTACACCA CTTAATCCTC CGCAAAAGAA AAAGGTGTCA 
TTGCTGGAAT ATAGAAAAAG ACAGAAAGAA GCAAGAAAAA GTGGCTCTAA ACAGGAAAAT 
TTATCTCTGG TCACTTTATC TCCTCATCCG GATGGTATTG TCAACTGCAC CCCTTCTGAA 
AGTTGTAGTG CTGTTGCAGA GTGTATTGAT CAAAATGACG CTAGCACTTG TTTAACTCAG 
CCATCTGAAG TCCAGTCTGT CCCTTCTGAA GAAACCGAAC AAACTTCTTC AGTAACTGAC 
ACCTCATCCA CTGAAAAAAA TGATCCTGAA GTGCAGTGGA CTGCATCAAC ATGTGTTGAG 
CAAGTCAGAG AGAGAAGCTA TCAAAGAGCT TTGTTACTGA GTGACCACAG AAAGGACAAA 
GATGATGCTG ACATAGAAGG TGGGGACCCT GCTAATGCCA AGGAATGCCA GTCACCAGAC 
ATATCCAGTA AGAATTGTAG AAGTCCTAAA CAGAGTAAGC CCTGTTCTCT GGTGCCTAAT 
TTATCTCATG GAAAGACGCC CTCAAAGCTA GATCCTGCCC AAGAAACCAT GGTGAATCCA 
TCAACAACTG CTGATGTTAA TTTTTGTAAC ACCGATTCTC AGCAGAAACA GCTCCTTTCT 
AAGTCTCTCA TTCCCCATGC TCAGAAGCTA CCTTCTACAC CTTTAAAGTT GCATTGTCCG 
TCATCTCCTC TTCTTGAACA CCCTCCAAAA TCTTCAACAC CAGTACAACA TGGTTATCTT 
TCACCAAAGC CTCATTCACA AAAATTAGGT TCTCCATATA GGCCACATCA TCCCCAGCAG 
CATCAGGTGG GAACACCACA AAGAGAGTCT CTTCCAAACT TTTACCCCGT AGTGCAAAGC 
CTTCAGCCTG CTAATCCACA GCCCTCTAGT GGCCAGTTGT TTTCCCAGAC CCAGCCAGGA 
CAGTGCTCCA ATGTTTATAC TCCCTTTAAT CAACAGACCG CCAGCAGTCC TGCTCCACCT 
CCCCCACCCC CACCTCCAAC AGCAACTTAC TATCAAAACC AGCAGCCCTC TATAAACTTC 
CAGAACTTTA GTCAGATAAA GGGAGCTGTG TCCCAACAAA CTGTGTTTAC ACCTGGTCCA 
AATCAAGGTC ATCCAACTGC AGCAGGACAA CCAGCAATTC AAGGACACCA TGTAAACTCT 
GGACATTATT TGCCTTCTCA AAGCCCTAAT GTACATCTCC AAGCTACAGG GGTTCCACCA 
CCCCCCCCGC CACCTCTTCC AAGCTGCCAT CAGCAGCACT CTGTTCAGCA TGTTACAAGC 
CCAGGTCATA CGGTTCCAGT AGCCAGTAGC TCACATATTC ATTCTCAGAC ACCAGGGCAT 
CATTTACCTC CACCACCCCC TCCACCACCT GGCCCAAGTC CTCATCATTC GCATCTTCAG 
CATGTATCAG CAGGACACCA AGGCCTGCAA GTGCAGCACC AGCATGTTTT AAATACAGTT 
CCTCCACCTC CCCCACCACC ACCTCCACCC CCTCCACAAG CAGCAAATGT TTTAGGTGGT 
GCTGGAAATC ACACGGCATC AGCACAAGTT ATTCATATGC AAACTCATCA AGGACCTGTT 
TTCTCTTCAG GAGCTCATCC TAGTGTAGCA CCATATTCAA CACAAGCTTC CCATCATAAC 
CAAGTTGGAC CTGTACCACA GCACCAGTCT TCTGCCACAG GACCGCATCT CCCACTTCCT 
GGCCAGAACT CTCATATTCA GACACAAGGA CCAAACAGTA TTGCAACACC TGCAGGTTTT 
TGTCCGCATC CTGGCTCTGT AAGCCTTGCT CATGGAGTTC AAGGACCTCA GCAGGCATCA 
ACAGTGCCTG GCCAGGTTCC TATTCATAGG CCACAGGTAC CTCCGACCTT CCAGAATAAT 
TACCATGGCT CAGGATGGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_018092321.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947810.2 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	OXa54852
Clone ID Related Accession (Same CDS sequence)	XM_018092321.2
Accession Version	XM_018092321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive histone-lysine N-methyltransferase 2E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGGTGACACC 
AGTGCAACAG AAAGTTGTGA TGAAGTTCCC ATGGAACTGT ACACTGCTTT CCAGCACACC 
CCAACAACTC TTACTCTCAC TGCCACTAGA GTAAATAAGG TTAATGACAA AAAGAGGAAG 
AAAAGTGGTG AGAAGGAGCA GAATAATTCT AAAGTTAAGA AGGCTTTTCG TGAAGGATCA 
AGAAAATCAT CACGTGTTAA GGGCTCTGCT CCAGAAATTG ATCCCTTAGA CAATTATGCT 
TTTGGATGGG AGAATAAAAT CAAGACTTGG ATGGACCACT ATGAAGAAGC AAGCAACAAC 
CAATACAGTG AAGGGGTTCA AAAAGAGGCA CAAAGGATTG CTTTGAGATT CAGTAACGAA 
AATGATAATA AAGAACAAAT AAAGACCAAC TTTAACACCA GCAATCTAAT TTTCAAGCCA 
CCGGTAGAGA GCTATCTACA AAAGAACAAA AAAATCTTAA AGGCTGCCAA AGACTTGCTT 
CCTGAAACAC TAATTATAGA ATACAGGGGA AAATTTATGC TCCGGGAGCA GTTTGAAGCA 
AATGGGTATT TCTTTAAAAG GCCATACCCT TTTATTTTGT TTTATTCTAA ATTTCATGGA 
TTAGAAATGT GTGTTGATGC AAGGACATTT GGAAATGAGG CTCGATTTAT ACGACGTTCC 
TGCACACCTA ATGCTGAGGT GCGACATGTT ATTGAAGAAG GAACTATTCA CCTATATATA 
TACTCCATTT CAAGCATTCC AAAAGGGGCA GAAATCACTA TTGCTTTTGA TTTTGATTAT 
GCAAATTGTA AATATAAGGT GGATTGTGCA TGCTTCAAAG ACAATCCAGA CTGCCCAGTT 
CTCCGATGCA GTACAGAGCC TACAGAAAAT AACAGTGGCT ATGAAACTAG AAGAAAAAAG 
GGGAAAAAGG AAAAAGAACT TTTAAAAGAA AGAGAATCTC AAAACCAGAA CATTGTAATA 
GATTCAGATG GAGCAATTAA CAAATTTAAA ACATCAGATA ACAAGCAAAG AAAGCTCTCC 
CCTCTTAGAT TGTCAATATC AAACAACCAG GAGCCAGATA TTAATGATGA TATAGAAGAA 
AATACTCCTC TTAGCAATGA CGTAGAAATG GAAACAGAGG AGCAGATTGC AGAAAGAAAA 
AGGAAGATGA CTAGAGAAGA AAGAAAAATG GAAGCTATTC TGCAAGCTTT TGCAAGACTG 
GAAAAAAGAG AAAAAAGAAG AGAACAAGCT TTAGAAAGAA TTGGCACTTC CAAACCAGAT 
GTGAAAACAG AGAGCAAGGA CTTACAGATT ATCAGTGAAT TGGAGCTCCC TCCGGAACCA 
GTTAAGGAAG AACCTGCCAG CAAACCTACT CCTGCCAAAA TAAATCGCAC AAAGCAGCGA 
AAGAGTTTTA CAAGAAGTAG GACTCATATT GGACAACAGC GTCGTCGGCA CCGGACTATA 
AGTACCTGCT CAGATATCCA GCCATCTTCC CCAGATGTTG AGGTTGTACC ACAGCAGATT 
GAAACAGAAA ATAATGCTGT ACCTGAAGAA CCTCCAAATG AAAGCAGTCC CCATAATACA 
GAAGAAGTTC CAGAGACAGA ATCTTGTGTT CAAAGCAAAT GTTCTATAAA GTACCCAAAA 
ACAAAAAAGC ACTTGGTAAA TGAATGGTTA AATGAAAAGC ACGAGAAAGC TGGCAAACCT 
GGAGAGCCCA TATTGGAAAG ACCTTTAAGA ATAACCACAG ATCCTGAAGT CCTTGCTACT 
CAGCTGAATT CCTTACCAGG TTTAATGTAT AGTCCTCATG TGTACACCAC TCCTAAGCAT 
TATATTAGGT TTACATCACC ATTCCTTTCT GAGAAAAAGA AGAAGAAAAA TGAAGAGAAT 
GTTTCCAGTT GTTGCAAAAA GCGTTGGTTG AAGAAAGCTC TGGAAGAAGA AAATTCTTCG 
GGGGTTAATC CATTTGGTTC TCCTGCTCAG GACCGATCTC AAAGTCCGGT TTCATTACAT 
GAAAATAAAT GCCATCTAAT AATGAATGGC AGCTCTCCCC TGGCAGATTT TACAACTCCA 
CTGAAGAAAC GAAGGTTGTT TAATTTTTTG GATTGCAATT TTTCCGAAAG TTCAACACCA 
GTTGCATCTC CTTATGCTAC CCCTACACAT ACTGATATTA CTTCCATGGA TACATCCTTA 
TTTACTACTC CACCCAGGGT AAAAGTAGAA GAGGAAATCT GTCGCAACGG TTGCAAGCAA 
ACCTACTCAC CAGTTACTCC AGTAACACCC TGTCTGCGTG GAAACAACAT GCATTTTGAG 
AGTATTTCTT CACCTGATAG TTCACCAGAA GTGAACCGAA GATCTTTTTG TCAGGAAGGA 
AATGACCGGG CATCTACAGC ATTGAATTCT TTCAGAAATA CCAGTTTGTC TGACATGGGG 
CTTCAAGAAA TAAAGACTTT TGGCTACTGC AGCCCTAGGA ATAGGACTGA TGTTTCTGGT 
CAGTGTCCTG GTGAAAATGA TGCTGCATCA GAGGTCCATT TTGGAGCAGA CATAAATGAA 
CATAGCTCTC TGCAAAAGGT CATGGAGCCT CCACAGGATA GGACTGATTT AAATAGCCTA 
CAGGAAACTG CTCTTAGTGG ACAAGGCACC ATGTATTCAA CTTGGATTAA AAGTCCAAAT 
AGAACAGGGG TGAACTTCTT GTCCATGAAT TCTAATTTAA GAGATTTAAC CCCTTCTCAC 
CAGCTTGAAG TTGGTGGTGG ATTCAGAATG AATGAATCCA AATGCTTGAT CCCAGAAGAC 
TCACGGGGCG TTTTCATAGA TGGGCCTATC TTCTGCTCTG CAGAGGAGGG AACTGTTTCT 
GGATTTGGAA GATCTCTTGG TGGTGATGGG ATATTAGACG GAAATGGTAC ACCACTTAAT 
CCTCCGCAAA AGAAAAAGGT GTCATTGCTG GAATATAGAA AAAGACAGAA AGAAGCAAGA 
AAAAGTGGCT CTAAACAGGA AAATTTATCT CTGGTCACTT TATCTCCTCA TCCGGATGGT 
ATTGTCAACT GCACCCCTTC TGAAAGTTGT AGTGCTGTTG CAGAGTGTAT TGATCAAAAT 
GACGCTAGCA CTTGTTTAAC TCAGCCATCT GAAGTCCAGT CTGTCCCTTC TGAAGAAACC 
GAACAAACTT CTTCAGTAAC TGACACCTCA TCCACTGAAA AAAATGATCC TGAAGTGCAG 
TGGACTGCAT CAACATGTGT TGAGCAAGTC AGAGAGAGAA GCTATCAAAG AGCTTTGTTA 
CTGAGTGACC ACAGAAAGGA CAAAGATGAT GCTGACATAG AAGGTGGGGA CCCTGCTAAT 
GCCAAGGAAT GCCAGTCACC AGACATATCC AGTAAGAATT GTAGAAGTCC TAAACAGAGT 
AAGCCCTGTT CTCTGGTGCC TAATTTATCT CATGGAAAGA CGCCCTCAAA GCTAGATCCT 
GCCCAAGAAA CCATGGTGAA TCCATCAACA ACTGCTGATG TTAATTTTTG TAACACCGAT 
TCTCAGCAGA AACAGCTCCT TTCTAAGTCT CTCATTCCCC ATGCTCAGAA GCTACCTTCT 
ACACCTTTAA AGTTGCATTG TCCGTCATCT CCTCTTCTTG AACACCCTCC AAAATCTTCA 
ACACCAGTAC AACATGGTTA TCTTTCACCA AAGCCTCATT CACAAAAATT AGGTTCTCCA 
TATAGGCCAC ATCATCCCCA GCAGCATCAG GTGGGAACAC CACAAAGAGA GTCTCTTCCA 
AACTTTTACC CCGTAGTGCA AAGCCTTCAG CCTGCTAATC CACAGCCCTC TAGTGGCCAG 
TTGTTTTCCC AGACCCAGCC AGGACAGTGC TCCAATGTTT ATACTCCCTT TAATCAACAG 
ACCGCCAGCA GTCCTGCTCC ACCTCCCCCA CCCCCACCTC CAACAGCAAC TTACTATCAA 
AACCAGCAGC CCTCTATAAA CTTCCAGAAC TTTAGTCAGA TAAAGGGAGC TGTGTCCCAA 
CAAACTGTGT TTACACCTGG TCCAAATCAA GGTCATCCAA CTGCAGCAGG ACAACCAGCA 
ATTCAAGGAC ACCATGTAAA CTCTGGACAT TATTTGCCTT CTCAAAGCCC TAATGTACAT 
CTCCAAGCTA CAGGGGTTCC ACCACCCCCC CCGCCACCTC TTCCAAGCTG CCATCAGCAG 
CACTCTGTTC AGCATGTTAC AAGCCCAGGT CATACGGTTC CAGTAGCCAG TAGCTCACAT 
ATTCATTCTC AGACACCAGG GCATCATTTA CCTCCACCAC CCCCTCCACC ACCTGGCCCA 
AGTCCTCATC ATTCGCATCT TCAGCATGTA TCAGCAGGAC ACCAAGGCCT GCAAGTGCAG 
CACCAGCATG TTTTAAATAC AGTTCCTCCA CCTCCCCCAC CACCACCTCC ACCCCCTCCA 
CAAGCAGCAA ATGTTTTAGG TGGTGCTGGA AATCACACGG CATCAGCACA AGTTATTCAT 
ATGCAAACTC ATCAAGGACC TGTTTTCTCT TCAGGAGCTC ATCCTAGTGT AGCACCATAT 
TCAACACAAG CTTCCCATCA TAACCAAGTT GGACCTGTAC CACAGCACCA GTCTTCTGCC 
ACAGGACCGC ATCTCCCACT TCCTGGCCAG AACTCTCATA TTCAGACACA AGGACCAAAC 
AGTATTGCAA CACCTGCAGG TTTTTGTCCG CATCCTGGCT CTGTAAGCCT TGCTCATGGA 
GTTCAAGGAC CTCAGCAGGC ATCAACAGTG CCTGGCCAGG TTCCTATTCA TAGGCCACAG 
GTACCTCCGA CCTTCCAGAA TAATTACCAT GGCTCAGGAT GGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947810.2
CDS	2078..7345
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSGTTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCRALDKERAVLLQQKKREHLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_018092321.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092321.2

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GGTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGGTGACACC 
AGTGCAACAG AAAGTTGTGA TGAAGTTCCC ATGGAACTGT ACACTGCTTT CCAGCACACC 
CCAACAACTC TTACTCTCAC TGCCACTAGA GTAAATAAGG TTAATGACAA AAAGAGGAAG 
AAAAGTGGTG AGAAGGAGCA GAATAATTCT AAAGTTAAGA AGGCTTTTCG TGAAGGATCA 
AGAAAATCAT CACGTGTTAA GGGCTCTGCT CCAGAAATTG ATCCCTTAGA CAATTATGCT 
TTTGGATGGG AGAATAAAAT CAAGACTTGG ATGGACCACT ATGAAGAAGC AAGCAACAAC 
CAATACAGTG AAGGGGTTCA AAAAGAGGCA CAAAGGATTG CTTTGAGATT CAGTAACGAA 
AATGATAATA AAGAACAAAT AAAGACCAAC TTTAACACCA GCAATCTAAT TTTCAAGCCA 
CCGGTAGAGA GCTATCTACA AAAGAACAAA AAAATCTTAA AGGCTGCCAA AGACTTGCTT 
CCTGAAACAC TAATTATAGA ATACAGGGGA AAATTTATGC TCCGGGAGCA GTTTGAAGCA 
AATGGGTATT TCTTTAAAAG GCCATACCCT TTTATTTTGT TTTATTCTAA ATTTCATGGA 
TTAGAAATGT GTGTTGATGC AAGGACATTT GGAAATGAGG CTCGATTTAT ACGACGTTCC 
TGCACACCTA ATGCTGAGGT GCGACATGTT ATTGAAGAAG GAACTATTCA CCTATATATA 
TACTCCATTT CAAGCATTCC AAAAGGGGCA GAAATCACTA TTGCTTTTGA TTTTGATTAT 
GCAAATTGTA AATATAAGGT GGATTGTGCA TGCTTCAAAG ACAATCCAGA CTGCCCAGTT 
CTCCGATGCA GTACAGAGCC TACAGAAAAT AACAGTGGCT ATGAAACTAG AAGAAAAAAG 
GGGAAAAAGG AAAAAGAACT TTTAAAAGAA AGAGAATCTC AAAACCAGAA CATTGTAATA 
GATTCAGATG GAGCAATTAA CAAATTTAAA ACATCAGATA ACAAGCAAAG AAAGCTCTCC 
CCTCTTAGAT TGTCAATATC AAACAACCAG GAGCCAGATA TTAATGATGA TATAGAAGAA 
AATACTCCTC TTAGCAATGA CGTAGAAATG GAAACAGAGG AGCAGATTGC AGAAAGAAAA 
AGGAAGATGA CTAGAGAAGA AAGAAAAATG GAAGCTATTC TGCAAGCTTT TGCAAGACTG 
GAAAAAAGAG AAAAAAGAAG AGAACAAGCT TTAGAAAGAA TTGGCACTTC CAAACCAGAT 
GTGAAAACAG AGAGCAAGGA CTTACAGATT ATCAGTGAAT TGGAGCTCCC TCCGGAACCA 
GTTAAGGAAG AACCTGCCAG CAAACCTACT CCTGCCAAAA TAAATCGCAC AAAGCAGCGA 
AAGAGTTTTA CAAGAAGTAG GACTCATATT GGACAACAGC GTCGTCGGCA CCGGACTATA 
AGTACCTGCT CAGATATCCA GCCATCTTCC CCAGATGTTG AGGTTGTACC ACAGCAGATT 
GAAACAGAAA ATAATGCTGT ACCTGAAGAA CCTCCAAATG AAAGCAGTCC CCATAATACA 
GAAGAAGTTC CAGAGACAGA ATCTTGTGTT CAAAGCAAAT GTTCTATAAA GTACCCAAAA 
ACAAAAAAGC ACTTGGTAAA TGAATGGTTA AATGAAAAGC ACGAGAAAGC TGGCAAACCT 
GGAGAGCCCA TATTGGAAAG ACCTTTAAGA ATAACCACAG ATCCTGAAGT CCTTGCTACT 
CAGCTGAATT CCTTACCAGG TTTAATGTAT AGTCCTCATG TGTACACCAC TCCTAAGCAT 
TATATTAGGT TTACATCACC ATTCCTTTCT GAGAAAAAGA AGAAGAAAAA TGAAGAGAAT 
GTTTCCAGTT GTTGCAAAAA GCGTTGGTTG AAGAAAGCTC TGGAAGAAGA AAATTCTTCG 
GGGGTTAATC CATTTGGTTC TCCTGCTCAG GACCGATCTC AAAGTCCGGT TTCATTACAT 
GAAAATAAAT GCCATCTAAT AATGAATGGC AGCTCTCCCC TGGCAGATTT TACAACTCCA 
CTGAAGAAAC GAAGGTTGTT TAATTTTTTG GATTGCAATT TTTCCGAAAG TTCAACACCA 
GTTGCATCTC CTTATGCTAC CCCTACACAT ACTGATATTA CTTCCATGGA TACATCCTTA 
TTTACTACTC CACCCAGGGT AAAAGTAGAA GAGGAAATCT GTCGCAACGG TTGCAAGCAA 
ACCTACTCAC CAGTTACTCC AGTAACACCC TGTCTGCGTG GAAACAACAT GCATTTTGAG 
AGTATTTCTT CACCTGATAG TTCACCAGAA GTGAACCGAA GATCTTTTTG TCAGGAAGGA 
AATGACCGGG CATCTACAGC ATTGAATTCT TTCAGAAATA CCAGTTTGTC TGACATGGGG 
CTTCAAGAAA TAAAGACTTT TGGCTACTGC AGCCCTAGGA ATAGGACTGA TGTTTCTGGT 
CAGTGTCCTG GTGAAAATGA TGCTGCATCA GAGGTCCATT TTGGAGCAGA CATAAATGAA 
CATAGCTCTC TGCAAAAGGT CATGGAGCCT CCACAGGATA GGACTGATTT AAATAGCCTA 
CAGGAAACTG CTCTTAGTGG ACAAGGCACC ATGTATTCAA CTTGGATTAA AAGTCCAAAT 
AGAACAGGGG TGAACTTCTT GTCCATGAAT TCTAATTTAA GAGATTTAAC CCCTTCTCAC 
CAGCTTGAAG TTGGTGGTGG ATTCAGAATG AATGAATCCA AATGCTTGAT CCCAGAAGAC 
TCACGGGGCG TTTTCATAGA TGGGCCTATC TTCTGCTCTG CAGAGGAGGG AACTGTTTCT 
GGATTTGGAA GATCTCTTGG TGGTGATGGG ATATTAGACG GAAATGGTAC ACCACTTAAT 
CCTCCGCAAA AGAAAAAGGT GTCATTGCTG GAATATAGAA AAAGACAGAA AGAAGCAAGA 
AAAAGTGGCT CTAAACAGGA AAATTTATCT CTGGTCACTT TATCTCCTCA TCCGGATGGT 
ATTGTCAACT GCACCCCTTC TGAAAGTTGT AGTGCTGTTG CAGAGTGTAT TGATCAAAAT 
GACGCTAGCA CTTGTTTAAC TCAGCCATCT GAAGTCCAGT CTGTCCCTTC TGAAGAAACC 
GAACAAACTT CTTCAGTAAC TGACACCTCA TCCACTGAAA AAAATGATCC TGAAGTGCAG 
TGGACTGCAT CAACATGTGT TGAGCAAGTC AGAGAGAGAA GCTATCAAAG AGCTTTGTTA 
CTGAGTGACC ACAGAAAGGA CAAAGATGAT GCTGACATAG AAGGTGGGGA CCCTGCTAAT 
GCCAAGGAAT GCCAGTCACC AGACATATCC AGTAAGAATT GTAGAAGTCC TAAACAGAGT 
AAGCCCTGTT CTCTGGTGCC TAATTTATCT CATGGAAAGA CGCCCTCAAA GCTAGATCCT 
GCCCAAGAAA CCATGGTGAA TCCATCAACA ACTGCTGATG TTAATTTTTG TAACACCGAT 
TCTCAGCAGA AACAGCTCCT TTCTAAGTCT CTCATTCCCC ATGCTCAGAA GCTACCTTCT 
ACACCTTTAA AGTTGCATTG TCCGTCATCT CCTCTTCTTG AACACCCTCC AAAATCTTCA 
ACACCAGTAC AACATGGTTA TCTTTCACCA AAGCCTCATT CACAAAAATT AGGTTCTCCA 
TATAGGCCAC ATCATCCCCA GCAGCATCAG GTGGGAACAC CACAAAGAGA GTCTCTTCCA 
AACTTTTACC CCGTAGTGCA AAGCCTTCAG CCTGCTAATC CACAGCCCTC TAGTGGCCAG 
TTGTTTTCCC AGACCCAGCC AGGACAGTGC TCCAATGTTT ATACTCCCTT TAATCAACAG 
ACCGCCAGCA GTCCTGCTCC ACCTCCCCCA CCCCCACCTC CAACAGCAAC TTACTATCAA 
AACCAGCAGC CCTCTATAAA CTTCCAGAAC TTTAGTCAGA TAAAGGGAGC TGTGTCCCAA 
CAAACTGTGT TTACACCTGG TCCAAATCAA GGTCATCCAA CTGCAGCAGG ACAACCAGCA 
ATTCAAGGAC ACCATGTAAA CTCTGGACAT TATTTGCCTT CTCAAAGCCC TAATGTACAT 
CTCCAAGCTA CAGGGGTTCC ACCACCCCCC CCGCCACCTC TTCCAAGCTG CCATCAGCAG 
CACTCTGTTC AGCATGTTAC AAGCCCAGGT CATACGGTTC CAGTAGCCAG TAGCTCACAT 
ATTCATTCTC AGACACCAGG GCATCATTTA CCTCCACCAC CCCCTCCACC ACCTGGCCCA 
AGTCCTCATC ATTCGCATCT TCAGCATGTA TCAGCAGGAC ACCAAGGCCT GCAAGTGCAG 
CACCAGCATG TTTTAAATAC AGTTCCTCCA CCTCCCCCAC CACCACCTCC ACCCCCTCCA 
CAAGCAGCAA ATGTTTTAGG TGGTGCTGGA AATCACACGG CATCAGCACA AGTTATTCAT 
ATGCAAACTC ATCAAGGACC TGTTTTCTCT TCAGGAGCTC ATCCTAGTGT AGCACCATAT 
TCAACACAAG CTTCCCATCA TAACCAAGTT GGACCTGTAC CACAGCACCA GTCTTCTGCC 
ACAGGACCGC ATCTCCCACT TCCTGGCCAG AACTCTCATA TTCAGACACA AGGACCAAAC 
AGTATTGCAA CACCTGCAGG TTTTTGTCCG CATCCTGGCT CTGTAAGCCT TGCTCATGGA 
GTTCAAGGAC CTCAGCAGGC ATCAACAGTG CCTGGCCAGG TTCCTATTCA TAGGCCACAG 
GTACCTCCGA CCTTCCAGAA TAATTACCAT GGCTCAGGAT GGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_012960003.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815457.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

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CloneID	OXa07888
Clone ID Related Accession (Same CDS sequence)	XM_012960003.2 , XM_004913017.3 , XM_018092319.1
Accession Version	XM_012960003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815457.1
CDS	364..5766
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_012960003.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960003.2

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_004913017.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913074.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

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CloneID	OXa07888
Clone ID Related Accession (Same CDS sequence)	XM_012960003.2 , XM_004913017.3 , XM_018092319.1
Accession Version	XM_004913017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913017.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913074.1
CDS	720..6122
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_004913017.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913017.3

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_018092319.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947808.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

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CloneID	OXa07888
Clone ID Related Accession (Same CDS sequence)	XM_012960003.2 , XM_004913017.3 , XM_018092319.1
Accession Version	XM_018092319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947808.1
CDS	716..6118
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_018092319.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092319.1

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGGAGCC AGATATTAAT 
GATGATATAG AAGAAAATAC TCCTCTTAGC AATGACGTAG AAATGGAAAC AGAGGAGCAG 
ATTGCAGAAA GAAAAAGGAA GATGACTAGA GAAGAAAGAA AAATGGAAGC TATTCTGCAA 
GCTTTTGCAA GACTGGAAAA AAGAGAAAAA AGAAGAGAAC AAGCTTTAGA AAGAATTGGC 
ACTTCCAAAC CAGACGTGAA AACAGAGAGC AAGGACTTAC AGATTATCAG TGAATTGGAG 
CTCCCTCCGG AACCAGTTAA GGAAGAACCT GCCAGCAAAC CTACTCCTGC CAAAATAAAT 
CGCACAAAGC AGCGAAAGAG TTTTACAAGA AGTAGGACTC ATATTGGACA ACAGCGTCGT 
CGGCACCGGA CTATAAGTAC CTGCTCAGAT ATCCAGCCAT CTTCCCCAGA TGTTGAGGTT 
GTACCACAGC AGATTGAAAC AGAAAATAAT GCTGTACCTG AAGAACCTCC AAATGAAAGC 
AGTCCCCATA ATACAGAAGA AGTTCCAGAG ACAGAATCTT GTGTTCAAAG CAAATGTTCT 
ATAAAGTACC CAAAAACAAA AAAGCACTTG GTAAATGAAT GGTTAAATGA AAAGCACGAG 
AAAGCTGGCA AACCTGGAGA GCCCATATTG GAAAGACCTT TAAGAATAAC CACAGATCCT 
GAAGTCCTTG CTACTCAGCT GAATTCCTTA CCAGGTTTAA TGTATAGTCC TCATGTGTAC 
ACCACTCCTA AGCATTATAT TAGGTTTACA TCACCATTCC TTTCTGAGAA AAAGAAGAAG 
AAAAATGAAG AGAATGTTTC CAGTTGTTGC AAAAAGCGTT GGTTGAAGAA AGCTCTGGAA 
GAAGAAAATT CTTCGGGGGT TAATCCATTT GGTTCTCCTG CTCAGGACCG ATCTCAAAGT 
CCGGTTTCAT TACATGAAAA TAAATGCCAT CTAATAATGA ATGGCAGCTC TCCCCTGGCA 
GATTTTACAA CTCCACTGAA GAAACGAAGG TTGTTTAATT TTTTGGATTG CAATTTTTCC 
GAAAGTTCAA CACCAGTTGC ATCTCCTTAT GCTACCCCTA CACATACTGA TATTACTTCC 
ATGGATACAT CCTTATTTAC TACTCCACCC AGGGTAAAAG TAGAAGAGGA AATCTGTCGC 
AACGGTTGCA AGCAAACCTA CTCACCAGTT ACTCCAGTAA CACCCTGTCT GCGTGGAAAC 
AACATGCATT TTGAGAGTAT TTCTTCACCT GATAGTTCAC CAGAAGTGAA CCGAAGATCT 
TTTTGTCAGG AAGGAAATGA CCGGGCATCT ACAGCATTGA ATTCTTTCAG AAATACCAGT 
TTGTCTGACA TGGGGCTTCA AGAAATAAAG ACTTTTGGCT ACTGCAGCCC TAGGAATAGG 
ACTGATGTTT CTGGTCAGTG TCCTGGTGAA AATGATGCTG CATCAGAGGT CCATTTTGGA 
GCAGACATAA ATGAACATAG CTCTCTGCAA AAGGTCATGG AGCCTCCACA GGATAGGACT 
GATTTAAATA GCCTACAGGA AACTGCTCTT AGTGGACAAG GCACCATGTA TTCAACTTGG 
ATTAAAAGTC CAAATAGAAC AGGGGTGAAC TTCTTGTCCA TGAATTCTAA TTTAAGAGAT 
TTAACCCCTT CTCACCAGCT TGAAGTTGGT GGTGGATTCA GAATGAATGA ATCCAAATGC 
TTGATCCCAG AAGACTCACG GGGCGTTTTC ATAGATGGGC CTATCTTCTG CTCTGCAGAG 
GAGGGAACTG TTTCTGGATT TGGAAGATCT CTTGGTGGTG ATGGGATATT AGACGGAAAT 
GGTACACCAC TTAATCCTCC GCAAAAGAAA AAGGTGTCAT TGCTGGAATA TAGAAAAAGA 
CAGAAAGAAG CAAGAAAAAG TGGCTCTAAA CAGGAAAATT TATCTCTGGT CACTTTATCT 
CCTCATCCGG ATGGTATTGT CAACTGCACC CCTTCTGAAA GTTGTAGTGC TGTTGCAGAG 
TGTATTGATC AAAATGACGC TAGCACTTGT TTAACTCAGC CATCTGAAGT CCAGTCTGTC 
CCTTCTGAAG AAACCGAACA AACTTCTTCA GTAACTGACA CCTCATCCAC TGAAAAAAAT 
GATCCTGAAG TGCAGTGGAC TGCATCAACA TGTGTTGAGC AAGTCAGAGA GAGAAGCTAT 
CAAAGAGCTT TGTTACTGAG TGACCACAGA AAGGACAAAG ATGATGCTGA CATAGAAGGT 
GGGGACCCTG CTAATGCCAA GGAATGCCAG TCACCAGACA TATCCAGTAA GAATTGTAGA 
AGTCCTAAAC AGAGTAAGCC CTGTTCTCTG GTGCCTAATT TATCTCATGG AAAGACGCCC 
TCAAAGCTAG ATCCTGCCCA AGAAACCATG GTGAATCCAT CAACAACTGC TGATGTTAAT 
TTTTGTAACA CCGATTCTCA GCAGAAACAG CTCCTTTCTA AGTCTCTCAT TCCCCATGCT 
CAGAAGCTAC CTTCTACACC TTTAAAGTTG CATTGTCCGT CATCTCCTCT TCTTGAACAC 
CCTCCAAAAT CTTCAACACC AGTACAACAT GGTTATCTTT CACCAAAGCC TCATTCACAA 
AAATTAGGTT CTCCATATAG GCCACATCAT CCCCAGCAGC ATCAGGTGGG AACACCACAA 
AGAGAGTCTC TTCCAAACTT TTACCCCGTA GTGCAAAGCC TTCAGCCTGC TAATCCACAG 
CCCTCTAGTG GCCAGTTGTT TTCCCAGACC CAGCCAGGAC AGTGCTCCAA TGTTTATACT 
CCCTTTAATC AACAGACCGC CAGCAGTCCT GCTCCACCTC CCCCACCCCC ACCTCCAACA 
GCAACTTACT ATCAAAACCA GCAGCCCTCT ATAAACTTCC AGAACTTTAG TCAGATAAAG 
GGAGCTGTGT CCCAACAAAC TGTGTTTACA CCTGGTCCAA ATCAAGGTCA TCCAACTGCA 
GCAGGACAAC CAGCAATTCA AGGACACCAT GTAAACTCTG GACATTATTT GCCTTCTCAA 
AGCCCTAATG TACATCTCCA AGCTACAGGG GTTCCACCAC CCCCACCGCC ACCTCTTCCA 
AGCTGCCATC AGCAGCACTC TGTTCAGCAT GTTACAAGCC CAGGTCATAC GGTTCCAGTA 
GCCAGTAGCT CACATATTCA TTCTCAGACA CCAGGGCATC ATTTACCTCC ACCACCCCCT 
CCACCACCTG GCCCAAGTCC TCATCATTCG CATCTTCAGC ATGTATCAGC AGGACACCAA 
GGCCTGCAAG TGCAGCACCA GCATGTTTTA AATACAGTTC CTCCACCTCC CCCACCACCA 
CCTCCACCCC CTCCACAAGC AGCAAATGTT TTAGGTGGTG CTGGAAATCA CACGGCATCA 
GCACAAGTTA TTCATATGCA AACTCATCAA GGACCTGTTT TCTCTTCAGG AGCTCATCCT 
AGTGTAGCAC CATATTCAAC ACAAGCTTCC CATCATAACC AAGTTGGACC TGTACCACAG 
CACCAGTCTT CTGCCACAGG ACCGCATCTC CCACTTCCTG GCCAGAACTC TCATATTCAG 
ACACAAGGAC CAAACAGTAT TGCAACACCT GCAGGTTTTT GTCCGCATCC TGGCTCTGTA 
AGCCTTGCTC ATGGAGTTCA AGGACCTCAG CAGGCATCAA CAGTGCCTGG CCAGGTTCCT 
ATTCATAGGC CACAGGTACC TCCGACCTTC CAGAATAATT ACCATGGCTC AGGATGGCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_004913019.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913076.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

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CloneID	OXa07890
Clone ID Related Accession (Same CDS sequence)	XM_004913019.3
Accession Version	XM_004913019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGACTAG AGAAGAAAGA 
AAAATGGAAG CTATTCTGCA AGCTTTTGCA AGACTGGAAA AAAGAGAAAA AAGAAGAGAA 
CAAGCTTTAG AAAGAATTGG CACTTCCAAA CCAGACGTGA AAACAGAGAG CAAGGACTTA 
CAGATTATCA GTGAATTGGA GCTCCCTCCG GAACCAGTTA AGGAAGAACC TGCCAGCAAA 
CCTACTCCTG CCAAAATAAA TCGCACAAAG CAGCGAAAGA GTTTTACAAG AAGTAGGACT 
CATATTGGAC AACAGCGTCG TCGGCACCGG ACTATAAGTA CCTGCTCAGA TATCCAGCCA 
TCTTCCCCAG ATGTTGAGGT TGTACCACAG CAGATTGAAA CAGAAAATAA TGCTGTACCT 
GAAGAACCTC CAAATGAAAG CAGTCCCCAT AATACAGAAG AAGTTCCAGA GACAGAATCT 
TGTGTTCAAA GCAAATGTTC TATAAAGTAC CCAAAAACAA AAAAGCACTT GGTAAATGAA 
TGGTTAAATG AAAAGCACGA GAAAGCTGGC AAACCTGGAG AGCCCATATT GGAAAGACCT 
TTAAGAATAA CCACAGATCC TGAAGTCCTT GCTACTCAGC TGAATTCCTT ACCAGGTTTA 
ATGTATAGTC CTCATGTGTA CACCACTCCT AAGCATTATA TTAGGTTTAC ATCACCATTC 
CTTTCTGAGA AAAAGAAGAA GAAAAATGAA GAGAATGTTT CCAGTTGTTG CAAAAAGCGT 
TGGTTGAAGA AAGCTCTGGA AGAAGAAAAT TCTTCGGGGG TTAATCCATT TGGTTCTCCT 
GCTCAGGACC GATCTCAAAG TCCGGTTTCA TTACATGAAA ATAAATGCCA TCTAATAATG 
AATGGCAGCT CTCCCCTGGC AGATTTTACA ACTCCACTGA AGAAACGAAG GTTGTTTAAT 
TTTTTGGATT GCAATTTTTC CGAAAGTTCA ACACCAGTTG CATCTCCTTA TGCTACCCCT 
ACACATACTG ATATTACTTC CATGGATACA TCCTTATTTA CTACTCCACC CAGGGTAAAA 
GTAGAAGAGG AAATCTGTCG CAACGGTTGC AAGCAAACCT ACTCACCAGT TACTCCAGTA 
ACACCCTGTC TGCGTGGAAA CAACATGCAT TTTGAGAGTA TTTCTTCACC TGATAGTTCA 
CCAGAAGTGA ACCGAAGATC TTTTTGTCAG GAAGGAAATG ACCGGGCATC TACAGCATTG 
AATTCTTTCA GAAATACCAG TTTGTCTGAC ATGGGGCTTC AAGAAATAAA GACTTTTGGC 
TACTGCAGCC CTAGGAATAG GACTGATGTT TCTGGTCAGT GTCCTGGTGA AAATGATGCT 
GCATCAGAGG TCCATTTTGG AGCAGACATA AATGAACATA GCTCTCTGCA AAAGGTCATG 
GAGCCTCCAC AGGATAGGAC TGATTTAAAT AGCCTACAGG AAACTGCTCT TAGTGGACAA 
GGCACCATGT ATTCAACTTG GATTAAAAGT CCAAATAGAA CAGGGGTGAA CTTCTTGTCC 
ATGAATTCTA ATTTAAGAGA TTTAACCCCT TCTCACCAGC TTGAAGTTGG TGGTGGATTC 
AGAATGAATG AATCCAAATG CTTGATCCCA GAAGACTCAC GGGGCGTTTT CATAGATGGG 
CCTATCTTCT GCTCTGCAGA GGAGGGAACT GTTTCTGGAT TTGGAAGATC TCTTGGTGGT 
GATGGGATAT TAGACGGAAA TGGTACACCA CTTAATCCTC CGCAAAAGAA AAAGGTGTCA 
TTGCTGGAAT ATAGAAAAAG ACAGAAAGAA GCAAGAAAAA GTGGCTCTAA ACAGGAAAAT 
TTATCTCTGG TCACTTTATC TCCTCATCCG GATGGTATTG TCAACTGCAC CCCTTCTGAA 
AGTTGTAGTG CTGTTGCAGA GTGTATTGAT CAAAATGACG CTAGCACTTG TTTAACTCAG 
CCATCTGAAG TCCAGTCTGT CCCTTCTGAA GAAACCGAAC AAACTTCTTC AGTAACTGAC 
ACCTCATCCA CTGAAAAAAA TGATCCTGAA GTGCAGTGGA CTGCATCAAC ATGTGTTGAG 
CAAGTCAGAG AGAGAAGCTA TCAAAGAGCT TTGTTACTGA GTGACCACAG AAAGGACAAA 
GATGATGCTG ACATAGAAGG TGGGGACCCT GCTAATGCCA AGGAATGCCA GTCACCAGAC 
ATATCCAGTA AGAATTGTAG AAGTCCTAAA CAGAGTAAGC CCTGTTCTCT GGTGCCTAAT 
TTATCTCATG GAAAGACGCC CTCAAAGCTA GATCCTGCCC AAGAAACCAT GGTGAATCCA 
TCAACAACTG CTGATGTTAA TTTTTGTAAC ACCGATTCTC AGCAGAAACA GCTCCTTTCT 
AAGTCTCTCA TTCCCCATGC TCAGAAGCTA CCTTCTACAC CTTTAAAGTT GCATTGTCCG 
TCATCTCCTC TTCTTGAACA CCCTCCAAAA TCTTCAACAC CAGTACAACA TGGTTATCTT 
TCACCAAAGC CTCATTCACA AAAATTAGGT TCTCCATATA GGCCACATCA TCCCCAGCAG 
CATCAGGTGG GAACACCACA AAGAGAGTCT CTTCCAAACT TTTACCCCGT AGTGCAAAGC 
CTTCAGCCTG CTAATCCACA GCCCTCTAGT GGCCAGTTGT TTTCCCAGAC CCAGCCAGGA 
CAGTGCTCCA ATGTTTATAC TCCCTTTAAT CAACAGACCG CCAGCAGTCC TGCTCCACCT 
CCCCCACCCC CACCTCCAAC AGCAACTTAC TATCAAAACC AGCAGCCCTC TATAAACTTC 
CAGAACTTTA GTCAGATAAA GGGAGCTGTG TCCCAACAAA CTGTGTTTAC ACCTGGTCCA 
AATCAAGGTC ATCCAACTGC AGCAGGACAA CCAGCAATTC AAGGACACCA TGTAAACTCT 
GGACATTATT TGCCTTCTCA AAGCCCTAAT GTACATCTCC AAGCTACAGG GGTTCCACCA 
CCCCCACCGC CACCTCTTCC AAGCTGCCAT CAGCAGCACT CTGTTCAGCA TGTTACAAGC 
CCAGGTCATA CGGTTCCAGT AGCCAGTAGC TCACATATTC ATTCTCAGAC ACCAGGGCAT 
CATTTACCTC CACCACCCCC TCCACCACCT GGCCCAAGTC CTCATCATTC GCATCTTCAG 
CATGTATCAG CAGGACACCA AGGCCTGCAA GTGCAGCACC AGCATGTTTT AAATACAGTT 
CCTCCACCTC CCCCACCACC ACCTCCACCC CCTCCACAAG CAGCAAATGT TTTAGGTGGT 
GCTGGAAATC ACACGGCATC AGCACAAGTT ATTCATATGC AAACTCATCA AGGACCTGTT 
TTCTCTTCAG GAGCTCATCC TAGTGTAGCA CCATATTCAA CACAAGCTTC CCATCATAAC 
CAAGTTGGAC CTGTACCACA GCACCAGTCT TCTGCCACAG GACCGCATCT CCCACTTCCT 
GGCCAGAACT CTCATATTCA GACACAAGGA CCAAACAGTA TTGCAACACC TGCAGGTTTT 
TGTCCGCATC CTGGCTCTGT AAGCCTTGCT CATGGAGTTC AAGGACCTCA GCAGGCATCA 
ACAGTGCCTG GCCAGGTTCC TATTCATAGG CCACAGGTAC CTCCGACCTT CCAGAATAAT 
TACCATGGCT CAGGATGGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913076.1
CDS	717..6020
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQNIPDIYLCERCQPRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_004913019.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913019.3

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG TGTCTGGCAA 
CATATTGATT GTATGGGTAT TGACAGGCAA AATATTCCAG ACATCTACTT ATGTGAGCGT 
TGTCAGCCTC GGGCTTTGGA TAAAGAGAGA GCAGTTTTAC TTCAGCAGAA AAAGCGAGAA 
CATTTGTCAG ATGATCTTCA AACCTGCTAC AATGGCAGAG GCAACAACGG AGGCCTTTTA 
TCTGATGGTG ACACCAGTGC AACAGAAAGT TGTGATGAAG TTCCCATGGA ACTGTACACT 
GCTTTCCAGC ACACCCCAAC AACTCTTACT CTCACTGCCA CTAGAGTAAA TAAGGTTAAT 
GACAAAAAGA GGAAGAAAAG TGGTGAGAAG GAGCAGAATA ATTCTAAAGT TAAGAAGGCT 
TTTCGTGAAG GATCAAGAAA ATCATCACGT GTTAAGGGCT CTGCTCCAGA AATTGATCCC 
TTAGACAATT ATGCTTTTGG ATGGGAGAAT AAAATCAAGA CTTGGATGGA CCACTATGAA 
GAAGCAAGCA ACAACCAATA CAGTGAAGGG GTTCAAAAAG AGGCACAAAG GATTGCTTTG 
AGATTCAGTA ACGAAAATGA TAATAAAGAA CAAATAAAGA CCAACTTTAA CACCAGCAAT 
CTAATTTTCA AGCCACCGGT AGAGAGCTAT CTACAAAAGA ACAAAAAAAT CTTAAAGGCT 
GCCAAAGACT TGCTTCCTGA AACACTAATT ATAGAATACA GGGGAAAATT TATGCTCCGG 
GAGCAGTTTG AAGCAAATGG GTATTTCTTT AAAAGGCCAT ACCCTTTTAT TTTGTTTTAT 
TCTAAATTTC ATGGATTAGA AATGTGTGTT GATGCAAGGA CATTTGGAAA TGAGGCTCGA 
TTTATACGAC GTTCCTGCAC ACCTAATGCT GAGGTGCGAC ATGTTATTGA AGAAGGAACT 
ATTCACCTAT ATATATACTC CATTTCAAGC ATTCCAAAAG GGGCAGAAAT CACTATTGCT 
TTTGATTTTG ATTATGCAAA TTGTAAATAT AAGGTGGATT GTGCATGCTT CAAAGACAAT 
CCAGACTGCC CAGTTCTCCG ATGCAGTACA GAGCCTACAG AAAATAACAG TGGCTATGAA 
ACTAGAAGAA AAAAGGGGAA AAAGGAAAAA GAACTTTTAA AAGAAAGAGA ATCTCAAAAC 
CAGAACATTG TAATAGATTC AGATGGAGCA ATTAACAAAT TTAAAACATC AGATAACAAG 
CAAAGAAAGC TCTCCCCTCT TAGATTGTCA ATATCAAACA ACCAGACTAG AGAAGAAAGA 
AAAATGGAAG CTATTCTGCA AGCTTTTGCA AGACTGGAAA AAAGAGAAAA AAGAAGAGAA 
CAAGCTTTAG AAAGAATTGG CACTTCCAAA CCAGACGTGA AAACAGAGAG CAAGGACTTA 
CAGATTATCA GTGAATTGGA GCTCCCTCCG GAACCAGTTA AGGAAGAACC TGCCAGCAAA 
CCTACTCCTG CCAAAATAAA TCGCACAAAG CAGCGAAAGA GTTTTACAAG AAGTAGGACT 
CATATTGGAC AACAGCGTCG TCGGCACCGG ACTATAAGTA CCTGCTCAGA TATCCAGCCA 
TCTTCCCCAG ATGTTGAGGT TGTACCACAG CAGATTGAAA CAGAAAATAA TGCTGTACCT 
GAAGAACCTC CAAATGAAAG CAGTCCCCAT AATACAGAAG AAGTTCCAGA GACAGAATCT 
TGTGTTCAAA GCAAATGTTC TATAAAGTAC CCAAAAACAA AAAAGCACTT GGTAAATGAA 
TGGTTAAATG AAAAGCACGA GAAAGCTGGC AAACCTGGAG AGCCCATATT GGAAAGACCT 
TTAAGAATAA CCACAGATCC TGAAGTCCTT GCTACTCAGC TGAATTCCTT ACCAGGTTTA 
ATGTATAGTC CTCATGTGTA CACCACTCCT AAGCATTATA TTAGGTTTAC ATCACCATTC 
CTTTCTGAGA AAAAGAAGAA GAAAAATGAA GAGAATGTTT CCAGTTGTTG CAAAAAGCGT 
TGGTTGAAGA AAGCTCTGGA AGAAGAAAAT TCTTCGGGGG TTAATCCATT TGGTTCTCCT 
GCTCAGGACC GATCTCAAAG TCCGGTTTCA TTACATGAAA ATAAATGCCA TCTAATAATG 
AATGGCAGCT CTCCCCTGGC AGATTTTACA ACTCCACTGA AGAAACGAAG GTTGTTTAAT 
TTTTTGGATT GCAATTTTTC CGAAAGTTCA ACACCAGTTG CATCTCCTTA TGCTACCCCT 
ACACATACTG ATATTACTTC CATGGATACA TCCTTATTTA CTACTCCACC CAGGGTAAAA 
GTAGAAGAGG AAATCTGTCG CAACGGTTGC AAGCAAACCT ACTCACCAGT TACTCCAGTA 
ACACCCTGTC TGCGTGGAAA CAACATGCAT TTTGAGAGTA TTTCTTCACC TGATAGTTCA 
CCAGAAGTGA ACCGAAGATC TTTTTGTCAG GAAGGAAATG ACCGGGCATC TACAGCATTG 
AATTCTTTCA GAAATACCAG TTTGTCTGAC ATGGGGCTTC AAGAAATAAA GACTTTTGGC 
TACTGCAGCC CTAGGAATAG GACTGATGTT TCTGGTCAGT GTCCTGGTGA AAATGATGCT 
GCATCAGAGG TCCATTTTGG AGCAGACATA AATGAACATA GCTCTCTGCA AAAGGTCATG 
GAGCCTCCAC AGGATAGGAC TGATTTAAAT AGCCTACAGG AAACTGCTCT TAGTGGACAA 
GGCACCATGT ATTCAACTTG GATTAAAAGT CCAAATAGAA CAGGGGTGAA CTTCTTGTCC 
ATGAATTCTA ATTTAAGAGA TTTAACCCCT TCTCACCAGC TTGAAGTTGG TGGTGGATTC 
AGAATGAATG AATCCAAATG CTTGATCCCA GAAGACTCAC GGGGCGTTTT CATAGATGGG 
CCTATCTTCT GCTCTGCAGA GGAGGGAACT GTTTCTGGAT TTGGAAGATC TCTTGGTGGT 
GATGGGATAT TAGACGGAAA TGGTACACCA CTTAATCCTC CGCAAAAGAA AAAGGTGTCA 
TTGCTGGAAT ATAGAAAAAG ACAGAAAGAA GCAAGAAAAA GTGGCTCTAA ACAGGAAAAT 
TTATCTCTGG TCACTTTATC TCCTCATCCG GATGGTATTG TCAACTGCAC CCCTTCTGAA 
AGTTGTAGTG CTGTTGCAGA GTGTATTGAT CAAAATGACG CTAGCACTTG TTTAACTCAG 
CCATCTGAAG TCCAGTCTGT CCCTTCTGAA GAAACCGAAC AAACTTCTTC AGTAACTGAC 
ACCTCATCCA CTGAAAAAAA TGATCCTGAA GTGCAGTGGA CTGCATCAAC ATGTGTTGAG 
CAAGTCAGAG AGAGAAGCTA TCAAAGAGCT TTGTTACTGA GTGACCACAG AAAGGACAAA 
GATGATGCTG ACATAGAAGG TGGGGACCCT GCTAATGCCA AGGAATGCCA GTCACCAGAC 
ATATCCAGTA AGAATTGTAG AAGTCCTAAA CAGAGTAAGC CCTGTTCTCT GGTGCCTAAT 
TTATCTCATG GAAAGACGCC CTCAAAGCTA GATCCTGCCC AAGAAACCAT GGTGAATCCA 
TCAACAACTG CTGATGTTAA TTTTTGTAAC ACCGATTCTC AGCAGAAACA GCTCCTTTCT 
AAGTCTCTCA TTCCCCATGC TCAGAAGCTA CCTTCTACAC CTTTAAAGTT GCATTGTCCG 
TCATCTCCTC TTCTTGAACA CCCTCCAAAA TCTTCAACAC CAGTACAACA TGGTTATCTT 
TCACCAAAGC CTCATTCACA AAAATTAGGT TCTCCATATA GGCCACATCA TCCCCAGCAG 
CATCAGGTGG GAACACCACA AAGAGAGTCT CTTCCAAACT TTTACCCCGT AGTGCAAAGC 
CTTCAGCCTG CTAATCCACA GCCCTCTAGT GGCCAGTTGT TTTCCCAGAC CCAGCCAGGA 
CAGTGCTCCA ATGTTTATAC TCCCTTTAAT CAACAGACCG CCAGCAGTCC TGCTCCACCT 
CCCCCACCCC CACCTCCAAC AGCAACTTAC TATCAAAACC AGCAGCCCTC TATAAACTTC 
CAGAACTTTA GTCAGATAAA GGGAGCTGTG TCCCAACAAA CTGTGTTTAC ACCTGGTCCA 
AATCAAGGTC ATCCAACTGC AGCAGGACAA CCAGCAATTC AAGGACACCA TGTAAACTCT 
GGACATTATT TGCCTTCTCA AAGCCCTAAT GTACATCTCC AAGCTACAGG GGTTCCACCA 
CCCCCACCGC CACCTCTTCC AAGCTGCCAT CAGCAGCACT CTGTTCAGCA TGTTACAAGC 
CCAGGTCATA CGGTTCCAGT AGCCAGTAGC TCACATATTC ATTCTCAGAC ACCAGGGCAT 
CATTTACCTC CACCACCCCC TCCACCACCT GGCCCAAGTC CTCATCATTC GCATCTTCAG 
CATGTATCAG CAGGACACCA AGGCCTGCAA GTGCAGCACC AGCATGTTTT AAATACAGTT 
CCTCCACCTC CCCCACCACC ACCTCCACCC CCTCCACAAG CAGCAAATGT TTTAGGTGGT 
GCTGGAAATC ACACGGCATC AGCACAAGTT ATTCATATGC AAACTCATCA AGGACCTGTT 
TTCTCTTCAG GAGCTCATCC TAGTGTAGCA CCATATTCAA CACAAGCTTC CCATCATAAC 
CAAGTTGGAC CTGTACCACA GCACCAGTCT TCTGCCACAG GACCGCATCT CCCACTTCCT 
GGCCAGAACT CTCATATTCA GACACAAGGA CCAAACAGTA TTGCAACACC TGCAGGTTTT 
TGTCCGCATC CTGGCTCTGT AAGCCTTGCT CATGGAGTTC AAGGACCTCA GCAGGCATCA 
ACAGTGCCTG GCCAGGTTCC TATTCATAGG CCACAGGTAC CTCCGACCTT CCAGAATAAT 
TACCATGGCT CAGGATGGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kmt2e ( XM_012960004.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815458.1 Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

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CloneID	OXa28975
Clone ID Related Accession (Same CDS sequence)	XM_012960004.2
Accession Version	XM_012960004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	histone-lysine N-methyltransferase 2E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGATCTTCAA 
ACCTGCTACA ATGGCAGAGG CAACAACGGA GGCCTTTTAT CTGATGGTGA CACCAGTGCA 
ACAGAAAGTT GTGATGAAGT TCCCATGGAA CTGTACACTG CTTTCCAGCA CACCCCAACA 
ACTCTTACTC TCACTGCCAC TAGAGTAAAT AAGGTTAATG ACAAAAAGAG GAAGAAAAGT 
GGTGAGAAGG AGCAGAATAA TTCTAAAGTT AAGAAGGCTT TTCGTGAAGG ATCAAGAAAA 
TCATCACGTG TTAAGGGCTC TGCTCCAGAA ATTGATCCCT TAGACAATTA TGCTTTTGGA 
TGGGAGAATA AAATCAAGAC TTGGATGGAC CACTATGAAG AAGCAAGCAA CAACCAATAC 
AGTGAAGGGG TTCAAAAAGA GGCACAAAGG ATTGCTTTGA GATTCAGTAA CGAAAATGAT 
AATAAAGAAC AAATAAAGAC CAACTTTAAC ACCAGCAATC TAATTTTCAA GCCACCGGTA 
GAGAGCTATC TACAAAAGAA CAAAAAAATC TTAAAGGCTG CCAAAGACTT GCTTCCTGAA 
ACACTAATTA TAGAATACAG GGGAAAATTT ATGCTCCGGG AGCAGTTTGA AGCAAATGGG 
TATTTCTTTA AAAGGCCATA CCCTTTTATT TTGTTTTATT CTAAATTTCA TGGATTAGAA 
ATGTGTGTTG ATGCAAGGAC ATTTGGAAAT GAGGCTCGAT TTATACGACG TTCCTGCACA 
CCTAATGCTG AGGTGCGACA TGTTATTGAA GAAGGAACTA TTCACCTATA TATATACTCC 
ATTTCAAGCA TTCCAAAAGG GGCAGAAATC ACTATTGCTT TTGATTTTGA TTATGCAAAT 
TGTAAATATA AGGTGGATTG TGCATGCTTC AAAGACAATC CAGACTGCCC AGTTCTCCGA 
TGCAGTACAG AGCCTACAGA AAATAACAGT GGCTATGAAA CTAGAAGAAA AAAGGGGAAA 
AAGGAAAAAG AACTTTTAAA AGAAAGAGAA TCTCAAAACC AGAACATTGT AATAGATTCA 
GATGGAGCAA TTAACAAATT TAAAACATCA GATAACAAGC AAAGAAAGCT CTCCCCTCTT 
AGATTGTCAA TATCAAACAA CCAGGAGCCA GATATTAATG ATGATATAGA AGAAAATACT 
CCTCTTAGCA ATGACGTAGA AATGGAAACA GAGGAGCAGA TTGCAGAAAG AAAAAGGAAG 
ATGACTAGAG AAGAAAGAAA AATGGAAGCT ATTCTGCAAG CTTTTGCAAG ACTGGAAAAA 
AGAGAAAAAA GAAGAGAACA AGCTTTAGAA AGAATTGGCA CTTCCAAACC AGACGTGAAA 
ACAGAGAGCA AGGACTTACA GATTATCAGT GAATTGGAGC TCCCTCCGGA ACCAGTTAAG 
GAAGAACCTG CCAGCAAACC TACTCCTGCC AAAATAAATC GCACAAAGCA GCGAAAGAGT 
TTTACAAGAA GTAGGACTCA TATTGGACAA CAGCGTCGTC GGCACCGGAC TATAAGTACC 
TGCTCAGATA TCCAGCCATC TTCCCCAGAT GTTGAGGTTG TACCACAGCA GATTGAAACA 
GAAAATAATG CTGTACCTGA AGAACCTCCA AATGAAAGCA GTCCCCATAA TACAGAAGAA 
GTTCCAGAGA CAGAATCTTG TGTTCAAAGC AAATGTTCTA TAAAGTACCC AAAAACAAAA 
AAGCACTTGG TAAATGAATG GTTAAATGAA AAGCACGAGA AAGCTGGCAA ACCTGGAGAG 
CCCATATTGG AAAGACCTTT AAGAATAACC ACAGATCCTG AAGTCCTTGC TACTCAGCTG 
AATTCCTTAC CAGGTTTAAT GTATAGTCCT CATGTGTACA CCACTCCTAA GCATTATATT 
AGGTTTACAT CACCATTCCT TTCTGAGAAA AAGAAGAAGA AAAATGAAGA GAATGTTTCC 
AGTTGTTGCA AAAAGCGTTG GTTGAAGAAA GCTCTGGAAG AAGAAAATTC TTCGGGGGTT 
AATCCATTTG GTTCTCCTGC TCAGGACCGA TCTCAAAGTC CGGTTTCATT ACATGAAAAT 
AAATGCCATC TAATAATGAA TGGCAGCTCT CCCCTGGCAG ATTTTACAAC TCCACTGAAG 
AAACGAAGGT TGTTTAATTT TTTGGATTGC AATTTTTCCG AAAGTTCAAC ACCAGTTGCA 
TCTCCTTATG CTACCCCTAC ACATACTGAT ATTACTTCCA TGGATACATC CTTATTTACT 
ACTCCACCCA GGGTAAAAGT AGAAGAGGAA ATCTGTCGCA ACGGTTGCAA GCAAACCTAC 
TCACCAGTTA CTCCAGTAAC ACCCTGTCTG CGTGGAAACA ACATGCATTT TGAGAGTATT 
TCTTCACCTG ATAGTTCACC AGAAGTGAAC CGAAGATCTT TTTGTCAGGA AGGAAATGAC 
CGGGCATCTA CAGCATTGAA TTCTTTCAGA AATACCAGTT TGTCTGACAT GGGGCTTCAA 
GAAATAAAGA CTTTTGGCTA CTGCAGCCCT AGGAATAGGA CTGATGTTTC TGGTCAGTGT 
CCTGGTGAAA ATGATGCTGC ATCAGAGGTC CATTTTGGAG CAGACATAAA TGAACATAGC 
TCTCTGCAAA AGGTCATGGA GCCTCCACAG GATAGGACTG ATTTAAATAG CCTACAGGAA 
ACTGCTCTTA GTGGACAAGG CACCATGTAT TCAACTTGGA TTAAAAGTCC AAATAGAACA 
GGGGTGAACT TCTTGTCCAT GAATTCTAAT TTAAGAGATT TAACCCCTTC TCACCAGCTT 
GAAGTTGGTG GTGGATTCAG AATGAATGAA TCCAAATGCT TGATCCCAGA AGACTCACGG 
GGCGTTTTCA TAGATGGGCC TATCTTCTGC TCTGCAGAGG AGGGAACTGT TTCTGGATTT 
GGAAGATCTC TTGGTGGTGA TGGGATATTA GACGGAAATG GTACACCACT TAATCCTCCG 
CAAAAGAAAA AGGTGTCATT GCTGGAATAT AGAAAAAGAC AGAAAGAAGC AAGAAAAAGT 
GGCTCTAAAC AGGAAAATTT ATCTCTGGTC ACTTTATCTC CTCATCCGGA TGGTATTGTC 
AACTGCACCC CTTCTGAAAG TTGTAGTGCT GTTGCAGAGT GTATTGATCA AAATGACGCT 
AGCACTTGTT TAACTCAGCC ATCTGAAGTC CAGTCTGTCC CTTCTGAAGA AACCGAACAA 
ACTTCTTCAG TAACTGACAC CTCATCCACT GAAAAAAATG ATCCTGAAGT GCAGTGGACT 
GCATCAACAT GTGTTGAGCA AGTCAGAGAG AGAAGCTATC AAAGAGCTTT GTTACTGAGT 
GACCACAGAA AGGACAAAGA TGATGCTGAC ATAGAAGGTG GGGACCCTGC TAATGCCAAG 
GAATGCCAGT CACCAGACAT ATCCAGTAAG AATTGTAGAA GTCCTAAACA GAGTAAGCCC 
TGTTCTCTGG TGCCTAATTT ATCTCATGGA AAGACGCCCT CAAAGCTAGA TCCTGCCCAA 
GAAACCATGG TGAATCCATC AACAACTGCT GATGTTAATT TTTGTAACAC CGATTCTCAG 
CAGAAACAGC TCCTTTCTAA GTCTCTCATT CCCCATGCTC AGAAGCTACC TTCTACACCT 
TTAAAGTTGC ATTGTCCGTC ATCTCCTCTT CTTGAACACC CTCCAAAATC TTCAACACCA 
GTACAACATG GTTATCTTTC ACCAAAGCCT CATTCACAAA AATTAGGTTC TCCATATAGG 
CCACATCATC CCCAGCAGCA TCAGGTGGGA ACACCACAAA GAGAGTCTCT TCCAAACTTT 
TACCCCGTAG TGCAAAGCCT TCAGCCTGCT AATCCACAGC CCTCTAGTGG CCAGTTGTTT 
TCCCAGACCC AGCCAGGACA GTGCTCCAAT GTTTATACTC CCTTTAATCA ACAGACCGCC 
AGCAGTCCTG CTCCACCTCC CCCACCCCCA CCTCCAACAG CAACTTACTA TCAAAACCAG 
CAGCCCTCTA TAAACTTCCA GAACTTTAGT CAGATAAAGG GAGCTGTGTC CCAACAAACT 
GTGTTTACAC CTGGTCCAAA TCAAGGTCAT CCAACTGCAG CAGGACAACC AGCAATTCAA 
GGACACCATG TAAACTCTGG ACATTATTTG CCTTCTCAAA GCCCTAATGT ACATCTCCAA 
GCTACAGGGG TTCCACCACC CCCACCGCCA CCTCTTCCAA GCTGCCATCA GCAGCACTCT 
GTTCAGCATG TTACAAGCCC AGGTCATACG GTTCCAGTAG CCAGTAGCTC ACATATTCAT 
TCTCAGACAC CAGGGCATCA TTTACCTCCA CCACCCCCTC CACCACCTGG CCCAAGTCCT 
CATCATTCGC ATCTTCAGCA TGTATCAGCA GGACACCAAG GCCTGCAAGT GCAGCACCAG 
CATGTTTTAA ATACAGTTCC TCCACCTCCC CCACCACCAC CTCCACCCCC TCCACAAGCA 
GCAAATGTTT TAGGTGGTGC TGGAAATCAC ACGGCATCAG CACAAGTTAT TCATATGCAA 
ACTCATCAAG GACCTGTTTT CTCTTCAGGA GCTCATCCTA GTGTAGCACC ATATTCAACA 
CAAGCTTCCC ATCATAACCA AGTTGGACCT GTACCACAGC ACCAGTCTTC TGCCACAGGA 
CCGCATCTCC CACTTCCTGG CCAGAACTCT CATATTCAGA CACAAGGACC AAACAGTATT 
GCAACACCTG CAGGTTTTTG TCCGCATCCT GGCTCTGTAA GCCTTGCTCA TGGAGTTCAA 
GGACCTCAGC AGGCATCAAC AGTGCCTGGC CAGGTTCCTA TTCATAGGCC ACAGGTACCT 
CCGACCTTCC AGAATAATTA CCATGGCTCA GGATGGCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815458.1
CDS	722..6043
Translation	MSIAIPLRVDTAEAPYLEMAAGSEPESVEASPVVIDKSNSYPHQLYTSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPVLSKNEVSIFTAPNFDETSSATTISTSEDGSYGTDVTRCICGFTHDDGYMICCDKCRALDKERAVLLQQKKREHLSDDLQTCYNGRGNNGGLLSDGDTSATESCDEVPMELYTAFQHTPTTLTLTATRVNKVNDKKRKKSGEKEQNNSKVKKAFREGSRKSSRVKGSAPEIDPLDNYAFGWENKIKTWMDHYEEASNNQYSEGVQKEAQRIALRFSNENDNKEQIKTNFNTSNLIFKPPVESYLQKNKKILKAAKDLLPETLIIEYRGKFMLREQFEANGYFFKRPYPFILFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHVIEEGTIHLYIYSISSIPKGAEITIAFDFDYANCKYKVDCACFKDNPDCPVLRCSTEPTENNSGYETRRKKGKKEKELLKERESQNQNIVIDSDGAINKFKTSDNKQRKLSPLRLSISNNQEPDINDDIEENTPLSNDVEMETEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERIGTSKPDVKTESKDLQIISELELPPEPVKEEPASKPTPAKINRTKQRKSFTRSRTHIGQQRRRHRTISTCSDIQPSSPDVEVVPQQIETENNAVPEEPPNESSPHNTEEVPETESCVQSKCSIKYPKTKKHLVNEWLNEKHEKAGKPGEPILERPLRITTDPEVLATQLNSLPGLMYSPHVYTTPKHYIRFTSPFLSEKKKKKNEENVSSCCKKRWLKKALEEENSSGVNPFGSPAQDRSQSPVSLHENKCHLIMNGSSPLADFTTPLKKRRLFNFLDCNFSESSTPVASPYATPTHTDITSMDTSLFTTPPRVKVEEEICRNGCKQTYSPVTPVTPCLRGNNMHFESISSPDSSPEVNRRSFCQEGNDRASTALNSFRNTSLSDMGLQEIKTFGYCSPRNRTDVSGQCPGENDAASEVHFGADINEHSSLQKVMEPPQDRTDLNSLQETALSGQGTMYSTWIKSPNRTGVNFLSMNSNLRDLTPSHQLEVGGGFRMNESKCLIPEDSRGVFIDGPIFCSAEEGTVSGFGRSLGGDGILDGNGTPLNPPQKKKVSLLEYRKRQKEARKSGSKQENLSLVTLSPHPDGIVNCTPSESCSAVAECIDQNDASTCLTQPSEVQSVPSEETEQTSSVTDTSSTEKNDPEVQWTASTCVEQVRERSYQRALLLSDHRKDKDDADIEGGDPANAKECQSPDISSKNCRSPKQSKPCSLVPNLSHGKTPSKLDPAQETMVNPSTTADVNFCNTDSQQKQLLSKSLIPHAQKLPSTPLKLHCPSSPLLEHPPKSSTPVQHGYLSPKPHSQKLGSPYRPHHPQQHQVGTPQRESLPNFYPVVQSLQPANPQPSSGQLFSQTQPGQCSNVYTPFNQQTASSPAPPPPPPPPTATYYQNQQPSINFQNFSQIKGAVSQQTVFTPGPNQGHPTAAGQPAIQGHHVNSGHYLPSQSPNVHLQATGVPPPPPPPLPSCHQQHSVQHVTSPGHTVPVASSSHIHSQTPGHHLPPPPPPPPGPSPHHSHLQHVSAGHQGLQVQHQHVLNTVPPPPPPPPPPPPQAANVLGGAGNHTASAQVIHMQTHQGPVFSSGAHPSVAPYSTQASHHNQVGPVPQHQSSATGPHLPLPGQNSHIQTQGPNSIATPAGFCPHPGSVSLAHGVQGPQQASTVPGQVPIHRPQVPPTFQNNYHGSGWH

Target ORF information:

RefSeq Version	XM_012960004.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific methyltransferase 2E (kmt2e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960004.2

ORF Insert Sequence:

ATGAGTATAG CGATCCCTTT GCGGGTTGAC ACAGCAGAGG CTCCTTATCT AGAAATGGCT 
GCAGGCTCAG AACCAGAATC TGTAGAAGCT AGCCCTGTCG TCATTGATAA GTCAAATAGT 
TATCCACATC AGTTATATAC CAGCAGTTCT CATCATTCGC ACAGTTATAT TGGATTGCCT 
TATGCGGATC ACAACTATGG TGCTCGCCCT CCACCAACAC CTCCAGCATC TCCTCCTCCT 
GTCCTTAGTA AGAATGAAGT AAGCATATTT ACTGCTCCTA ACTTTGATGA GACTTCCAGT 
GCTACAACAA TAAGTACTTC AGAAGATGGG AGCTATGGAA CTGATGTCAC TAGATGTATC 
TGCGGTTTTA CTCATGATGA TGGATATATG ATTTGTTGTG ACAAATGCAG GGCTTTGGAT 
AAAGAGAGAG CAGTTTTACT TCAGCAGAAA AAGCGAGAAC ATTTGTCAGA TGATCTTCAA 
ACCTGCTACA ATGGCAGAGG CAACAACGGA GGCCTTTTAT CTGATGGTGA CACCAGTGCA 
ACAGAAAGTT GTGATGAAGT TCCCATGGAA CTGTACACTG CTTTCCAGCA CACCCCAACA 
ACTCTTACTC TCACTGCCAC TAGAGTAAAT AAGGTTAATG ACAAAAAGAG GAAGAAAAGT 
GGTGAGAAGG AGCAGAATAA TTCTAAAGTT AAGAAGGCTT TTCGTGAAGG ATCAAGAAAA 
TCATCACGTG TTAAGGGCTC TGCTCCAGAA ATTGATCCCT TAGACAATTA TGCTTTTGGA 
TGGGAGAATA AAATCAAGAC TTGGATGGAC CACTATGAAG AAGCAAGCAA CAACCAATAC 
AGTGAAGGGG TTCAAAAAGA GGCACAAAGG ATTGCTTTGA GATTCAGTAA CGAAAATGAT 
AATAAAGAAC AAATAAAGAC CAACTTTAAC ACCAGCAATC TAATTTTCAA GCCACCGGTA 
GAGAGCTATC TACAAAAGAA CAAAAAAATC TTAAAGGCTG CCAAAGACTT GCTTCCTGAA 
ACACTAATTA TAGAATACAG GGGAAAATTT ATGCTCCGGG AGCAGTTTGA AGCAAATGGG 
TATTTCTTTA AAAGGCCATA CCCTTTTATT TTGTTTTATT CTAAATTTCA TGGATTAGAA 
ATGTGTGTTG ATGCAAGGAC ATTTGGAAAT GAGGCTCGAT TTATACGACG TTCCTGCACA 
CCTAATGCTG AGGTGCGACA TGTTATTGAA GAAGGAACTA TTCACCTATA TATATACTCC 
ATTTCAAGCA TTCCAAAAGG GGCAGAAATC ACTATTGCTT TTGATTTTGA TTATGCAAAT 
TGTAAATATA AGGTGGATTG TGCATGCTTC AAAGACAATC CAGACTGCCC AGTTCTCCGA 
TGCAGTACAG AGCCTACAGA AAATAACAGT GGCTATGAAA CTAGAAGAAA AAAGGGGAAA 
AAGGAAAAAG AACTTTTAAA AGAAAGAGAA TCTCAAAACC AGAACATTGT AATAGATTCA 
GATGGAGCAA TTAACAAATT TAAAACATCA GATAACAAGC AAAGAAAGCT CTCCCCTCTT 
AGATTGTCAA TATCAAACAA CCAGGAGCCA GATATTAATG ATGATATAGA AGAAAATACT 
CCTCTTAGCA ATGACGTAGA AATGGAAACA GAGGAGCAGA TTGCAGAAAG AAAAAGGAAG 
ATGACTAGAG AAGAAAGAAA AATGGAAGCT ATTCTGCAAG CTTTTGCAAG ACTGGAAAAA 
AGAGAAAAAA GAAGAGAACA AGCTTTAGAA AGAATTGGCA CTTCCAAACC AGACGTGAAA 
ACAGAGAGCA AGGACTTACA GATTATCAGT GAATTGGAGC TCCCTCCGGA ACCAGTTAAG 
GAAGAACCTG CCAGCAAACC TACTCCTGCC AAAATAAATC GCACAAAGCA GCGAAAGAGT 
TTTACAAGAA GTAGGACTCA TATTGGACAA CAGCGTCGTC GGCACCGGAC TATAAGTACC 
TGCTCAGATA TCCAGCCATC TTCCCCAGAT GTTGAGGTTG TACCACAGCA GATTGAAACA 
GAAAATAATG CTGTACCTGA AGAACCTCCA AATGAAAGCA GTCCCCATAA TACAGAAGAA 
GTTCCAGAGA CAGAATCTTG TGTTCAAAGC AAATGTTCTA TAAAGTACCC AAAAACAAAA 
AAGCACTTGG TAAATGAATG GTTAAATGAA AAGCACGAGA AAGCTGGCAA ACCTGGAGAG 
CCCATATTGG AAAGACCTTT AAGAATAACC ACAGATCCTG AAGTCCTTGC TACTCAGCTG 
AATTCCTTAC CAGGTTTAAT GTATAGTCCT CATGTGTACA CCACTCCTAA GCATTATATT 
AGGTTTACAT CACCATTCCT TTCTGAGAAA AAGAAGAAGA AAAATGAAGA GAATGTTTCC 
AGTTGTTGCA AAAAGCGTTG GTTGAAGAAA GCTCTGGAAG AAGAAAATTC TTCGGGGGTT 
AATCCATTTG GTTCTCCTGC TCAGGACCGA TCTCAAAGTC CGGTTTCATT ACATGAAAAT 
AAATGCCATC TAATAATGAA TGGCAGCTCT CCCCTGGCAG ATTTTACAAC TCCACTGAAG 
AAACGAAGGT TGTTTAATTT TTTGGATTGC AATTTTTCCG AAAGTTCAAC ACCAGTTGCA 
TCTCCTTATG CTACCCCTAC ACATACTGAT ATTACTTCCA TGGATACATC CTTATTTACT 
ACTCCACCCA GGGTAAAAGT AGAAGAGGAA ATCTGTCGCA ACGGTTGCAA GCAAACCTAC 
TCACCAGTTA CTCCAGTAAC ACCCTGTCTG CGTGGAAACA ACATGCATTT TGAGAGTATT 
TCTTCACCTG ATAGTTCACC AGAAGTGAAC CGAAGATCTT TTTGTCAGGA AGGAAATGAC 
CGGGCATCTA CAGCATTGAA TTCTTTCAGA AATACCAGTT TGTCTGACAT GGGGCTTCAA 
GAAATAAAGA CTTTTGGCTA CTGCAGCCCT AGGAATAGGA CTGATGTTTC TGGTCAGTGT 
CCTGGTGAAA ATGATGCTGC ATCAGAGGTC CATTTTGGAG CAGACATAAA TGAACATAGC 
TCTCTGCAAA AGGTCATGGA GCCTCCACAG GATAGGACTG ATTTAAATAG CCTACAGGAA 
ACTGCTCTTA GTGGACAAGG CACCATGTAT TCAACTTGGA TTAAAAGTCC AAATAGAACA 
GGGGTGAACT TCTTGTCCAT GAATTCTAAT TTAAGAGATT TAACCCCTTC TCACCAGCTT 
GAAGTTGGTG GTGGATTCAG AATGAATGAA TCCAAATGCT TGATCCCAGA AGACTCACGG 
GGCGTTTTCA TAGATGGGCC TATCTTCTGC TCTGCAGAGG AGGGAACTGT TTCTGGATTT 
GGAAGATCTC TTGGTGGTGA TGGGATATTA GACGGAAATG GTACACCACT TAATCCTCCG 
CAAAAGAAAA AGGTGTCATT GCTGGAATAT AGAAAAAGAC AGAAAGAAGC AAGAAAAAGT 
GGCTCTAAAC AGGAAAATTT ATCTCTGGTC ACTTTATCTC CTCATCCGGA TGGTATTGTC 
AACTGCACCC CTTCTGAAAG TTGTAGTGCT GTTGCAGAGT GTATTGATCA AAATGACGCT 
AGCACTTGTT TAACTCAGCC ATCTGAAGTC CAGTCTGTCC CTTCTGAAGA AACCGAACAA 
ACTTCTTCAG TAACTGACAC CTCATCCACT GAAAAAAATG ATCCTGAAGT GCAGTGGACT 
GCATCAACAT GTGTTGAGCA AGTCAGAGAG AGAAGCTATC AAAGAGCTTT GTTACTGAGT 
GACCACAGAA AGGACAAAGA TGATGCTGAC ATAGAAGGTG GGGACCCTGC TAATGCCAAG 
GAATGCCAGT CACCAGACAT ATCCAGTAAG AATTGTAGAA GTCCTAAACA GAGTAAGCCC 
TGTTCTCTGG TGCCTAATTT ATCTCATGGA AAGACGCCCT CAAAGCTAGA TCCTGCCCAA 
GAAACCATGG TGAATCCATC AACAACTGCT GATGTTAATT TTTGTAACAC CGATTCTCAG 
CAGAAACAGC TCCTTTCTAA GTCTCTCATT CCCCATGCTC AGAAGCTACC TTCTACACCT 
TTAAAGTTGC ATTGTCCGTC ATCTCCTCTT CTTGAACACC CTCCAAAATC TTCAACACCA 
GTACAACATG GTTATCTTTC ACCAAAGCCT CATTCACAAA AATTAGGTTC TCCATATAGG 
CCACATCATC CCCAGCAGCA TCAGGTGGGA ACACCACAAA GAGAGTCTCT TCCAAACTTT 
TACCCCGTAG TGCAAAGCCT TCAGCCTGCT AATCCACAGC CCTCTAGTGG CCAGTTGTTT 
TCCCAGACCC AGCCAGGACA GTGCTCCAAT GTTTATACTC CCTTTAATCA ACAGACCGCC 
AGCAGTCCTG CTCCACCTCC CCCACCCCCA CCTCCAACAG CAACTTACTA TCAAAACCAG 
CAGCCCTCTA TAAACTTCCA GAACTTTAGT CAGATAAAGG GAGCTGTGTC CCAACAAACT 
GTGTTTACAC CTGGTCCAAA TCAAGGTCAT CCAACTGCAG CAGGACAACC AGCAATTCAA 
GGACACCATG TAAACTCTGG ACATTATTTG CCTTCTCAAA GCCCTAATGT ACATCTCCAA 
GCTACAGGGG TTCCACCACC CCCACCGCCA CCTCTTCCAA GCTGCCATCA GCAGCACTCT 
GTTCAGCATG TTACAAGCCC AGGTCATACG GTTCCAGTAG CCAGTAGCTC ACATATTCAT 
TCTCAGACAC CAGGGCATCA TTTACCTCCA CCACCCCCTC CACCACCTGG CCCAAGTCCT 
CATCATTCGC ATCTTCAGCA TGTATCAGCA GGACACCAAG GCCTGCAAGT GCAGCACCAG 
CATGTTTTAA ATACAGTTCC TCCACCTCCC CCACCACCAC CTCCACCCCC TCCACAAGCA 
GCAAATGTTT TAGGTGGTGC TGGAAATCAC ACGGCATCAG CACAAGTTAT TCATATGCAA 
ACTCATCAAG GACCTGTTTT CTCTTCAGGA GCTCATCCTA GTGTAGCACC ATATTCAACA 
CAAGCTTCCC ATCATAACCA AGTTGGACCT GTACCACAGC ACCAGTCTTC TGCCACAGGA 
CCGCATCTCC CACTTCCTGG CCAGAACTCT CATATTCAGA CACAAGGACC AAACAGTATT 
GCAACACCTG CAGGTTTTTG TCCGCATCCT GGCTCTGTAA GCCTTGCTCA TGGAGTTCAA 
GGACCTCAGC AGGCATCAAC AGTGCCTGGC CAGGTTCCTA TTCATAGGCC ACAGGTACCT 
CCGACCTTCC AGAATAATTA CCATGGCTCA GGATGGCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kmt2e
Entrez Gene ID	779539
Full Name	lysine (K)-specific methyltransferase 2E
Synonyms	mll5
General protein information	Preferred Names lysine (K)-specific methyltransferase 2E Names histone-lysine N-methyltransferase 2E myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog) myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Bos taurus (cattle)	MLL5	NP_001075920.2
Mus musculus (house mouse)	Kmt2e	NP_081260.1
Rattus norvegicus (Norway rat)	Kmt2e	NP_001094321.1
Pan troglodytes (chimpanzee)	MLL5	XP_003318779.1
Gallus gallus (chicken)	MLL5	XP_003640389.1
Danio rerio (zebrafish)	kmt2e	XP_692965.5
Xenopus tropicalis (tropical clawed frog)	kmt2e	XP_004913074.1
Homo sapiens (human)	KMT2E	NP_891847.1
	LOC697233	XP_002808408.1
Canis lupus familiaris (dog)	KMT2E	XP_533102.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kmt2e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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