fam135a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following fam135a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fam135a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa44720 XM_018094346.1
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa59289 XM_004914504.4
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59290 XM_004914505.4
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59291 XM_004914506.4
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59292 XM_018094346.2
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa59287 XM_031902991.1
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59287 XM_031902992.1
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa59288 XM_004914503.4
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa10377 XM_018094345.1
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10377 XM_004914503.3
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa10378 XM_004914504.3
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10379 XM_004914505.3
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10380 XM_004914506.3
Latest version!
Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa44720
    Clone ID Related Accession (Same CDS sequence) XM_018094346.1
    Accession Version XM_018094346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA
    TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA
    AAACAGCCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT
    TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC
    TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT
    GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC
    GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA
    TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT
    CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT TTCCAGGATC
    AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA
    TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT
    ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA
    AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC
    TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC
    TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT
    CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT
    GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT
    ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT
    TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949835.1
    CDS284..4348
    Translation

    Target ORF information:

    RefSeq Version XM_018094346.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA
    TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA
    AAACAGCCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT
    TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC
    TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT
    GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC
    GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA
    TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT
    CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT TTCCAGGATC
    AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA
    TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT
    ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA
    AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC
    TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC
    TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT
    CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT
    GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT
    ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT
    TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59289
    Clone ID Related Accession (Same CDS sequence) XM_004914504.4
    Accession Version XM_004914504.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA
    TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT
    GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC
    ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC
    ACAAAACAAC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG
    CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT
    TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA
    GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT
    AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG
    GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT
    GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTATCCAGG
    ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC
    AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG
    GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG
    AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA
    TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG
    AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT
    GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA
    CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC
    AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA
    TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914561.1
    CDS279..4466
    Translation

    Target ORF information:

    RefSeq Version XM_004914504.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914504.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA
    TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT
    GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC
    ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC
    ACAAAACAAC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG
    CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT
    TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA
    GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT
    AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG
    GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT
    GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTATCCAGG
    ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC
    AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG
    GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG
    AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA
    TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG
    AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT
    GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA
    CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC
    AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA
    TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59290
    Clone ID Related Accession (Same CDS sequence) XM_004914505.4
    Accession Version XM_004914505.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC
    AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT
    CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG
    GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC
    AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT
    CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT
    GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC
    AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA
    TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT
    GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA
    CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT
    TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG
    TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG
    GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA
    GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG
    TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA
    GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT
    GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC
    GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT
    TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT
    GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT
    GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC
    TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT
    GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA
    ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT
    GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA
    AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC
    GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA
    TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAAC CGGGTGGTGC AACATCTTCT
    TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG
    GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC
    TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG
    ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC
    ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT
    GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT
    ATCCAAATTT ACAGTCTGTC AGTATCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT
    TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT
    ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT
    AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG
    TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC
    CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT
    CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT
    TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA
    GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA
    GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT
    CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914562.1
    CDS279..4436
    Translation

    Target ORF information:

    RefSeq Version XM_004914505.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914505.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC
    AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT
    CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG
    GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC
    AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT
    CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT
    GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC
    AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA
    TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT
    GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA
    CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT
    TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG
    TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG
    GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA
    GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG
    TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA
    GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT
    GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC
    GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT
    TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT
    GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT
    GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC
    TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT
    GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA
    ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT
    GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA
    AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC
    GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA
    TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAAC CGGGTGGTGC AACATCTTCT
    TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG
    GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC
    TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG
    ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC
    ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT
    GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT
    ATCCAAATTT ACAGTCTGTC AGTATCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT
    TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT
    ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT
    AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG
    TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC
    CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT
    CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT
    TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA
    GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA
    GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT
    CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59291
    Clone ID Related Accession (Same CDS sequence) XM_004914506.4
    Accession Version XM_004914506.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914506.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT
    ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT
    GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG
    AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT
    GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG
    GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC
    ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG
    TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT
    ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT
    ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA
    GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA
    ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC
    AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC
    ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA
    AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC
    AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC
    ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC
    ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA
    ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT
    GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT
    AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT
    AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT
    ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA
    AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA
    GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT
    GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG
    CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC
    TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG
    ATTGACACTA TCACAAAACA ACCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA
    AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG
    AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT
    AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT
    CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT
    GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT
    GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG
    TCAGTATCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT
    CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT
    GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT
    ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT
    CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT
    GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA
    ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA
    AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT
    CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG
    GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914563.1
    CDS279..4358
    Translation

    Target ORF information:

    RefSeq Version XM_004914506.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914506.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT
    ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT
    GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG
    AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT
    GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG
    GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC
    ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG
    TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT
    ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT
    ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA
    GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA
    ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC
    AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC
    ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA
    AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC
    AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC
    ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC
    ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA
    ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT
    GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT
    AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT
    AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT
    ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA
    AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA
    GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT
    GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG
    CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC
    TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG
    ATTGACACTA TCACAAAACA ACCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA
    AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG
    AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT
    AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT
    CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT
    GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT
    GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG
    TCAGTATCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT
    CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT
    GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT
    ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT
    CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT
    GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA
    ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA
    AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT
    CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG
    GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59292
    Clone ID Related Accession (Same CDS sequence) XM_018094346.2
    Accession Version XM_018094346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA
    TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA
    AAACAACCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT
    TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC
    TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT
    GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC
    GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA
    TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT
    CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT ATCCAGGATC
    AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA
    TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT
    ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA
    AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC
    TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC
    TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT
    CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT
    GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT
    ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT
    TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949835.1
    CDS279..4343
    Translation

    Target ORF information:

    RefSeq Version XM_018094346.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA
    TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA
    AAACAACCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT
    TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC
    TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT
    GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC
    GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA
    TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT
    CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT ATCCAGGATC
    AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA
    TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT
    ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA
    AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC
    TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC
    TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT
    CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT
    GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT
    ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT
    TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59287
    Clone ID Related Accession (Same CDS sequence) XM_031902991.1 , XM_031902992.1
    Accession Version XM_031902991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA
    AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT
    AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG
    TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG
    GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC
    TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG
    AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT
    TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG
    CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA
    AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT
    CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG
    CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT
    AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA
    AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT
    GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT
    GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA
    GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA
    CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG
    AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT
    TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG
    GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC
    TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT
    TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC
    AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT
    GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA
    CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA
    TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC
    CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT
    GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC
    GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA
    GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT
    GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG
    TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA
    ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT
    TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT
    GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT
    CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT
    CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA
    GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT
    GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA
    AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA
    ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT
    TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC
    AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC
    AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG
    TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC
    AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG
    TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG
    TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG
    GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT
    ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT
    CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758851.1
    CDS280..4869
    Translation

    Target ORF information:

    RefSeq Version XM_031902991.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA
    AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT
    AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG
    TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG
    GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC
    TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG
    AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT
    TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG
    CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA
    AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT
    CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG
    CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT
    AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA
    AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT
    GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT
    GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA
    GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA
    CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG
    AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT
    TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG
    GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC
    TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT
    TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC
    AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT
    GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA
    CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA
    TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC
    CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT
    GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC
    GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA
    GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT
    GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG
    TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA
    ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT
    TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT
    GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT
    CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT
    CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA
    GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT
    GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA
    AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA
    ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT
    TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC
    AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC
    AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG
    TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC
    AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG
    TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG
    TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG
    GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT
    ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT
    CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59287
    Clone ID Related Accession (Same CDS sequence) XM_031902991.1 , XM_031902992.1
    Accession Version XM_031902992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA
    AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT
    AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG
    TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG
    GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC
    TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG
    AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT
    TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG
    CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA
    AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT
    CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG
    CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT
    AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA
    AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT
    GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT
    GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA
    GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA
    CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG
    AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT
    TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG
    GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC
    TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT
    TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC
    AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT
    GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA
    CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA
    TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC
    CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT
    GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC
    GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA
    GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT
    GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG
    TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA
    ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT
    TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT
    GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT
    CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT
    CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA
    GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT
    GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA
    AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA
    ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT
    TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC
    AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC
    AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG
    TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC
    AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG
    TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG
    TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG
    GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT
    ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT
    CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758852.1
    CDS277..4866
    Translation

    Target ORF information:

    RefSeq Version XM_031902992.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA
    AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT
    AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG
    TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG
    GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC
    TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG
    AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT
    TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG
    CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA
    AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT
    CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG
    CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT
    AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA
    AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT
    GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT
    GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA
    GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA
    CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG
    AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT
    TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG
    GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC
    TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT
    TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC
    AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT
    GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA
    CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA
    TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC
    CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT
    GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC
    GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA
    GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT
    GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG
    TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA
    ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT
    TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT
    GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT
    CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT
    CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA
    GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT
    GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA
    AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA
    ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT
    TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC
    AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC
    AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG
    TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC
    AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG
    TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG
    TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG
    GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT
    ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT
    CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59288
    Clone ID Related Accession (Same CDS sequence) XM_004914503.4
    Accession Version XM_004914503.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914503.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAACCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTATC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914560.1
    CDS280..4791
    Translation

    Target ORF information:

    RefSeq Version XM_004914503.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914503.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAACCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTATC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10377
    Clone ID Related Accession (Same CDS sequence) XM_004914503.3 , XM_018094345.1
    Accession Version XM_004914503.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914560.1
    CDS285..4796
    Translation

    Target ORF information:

    RefSeq Version XM_004914503.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914503.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10377
    Clone ID Related Accession (Same CDS sequence) XM_004914503.3 , XM_018094345.1
    Accession Version XM_018094345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949834.1
    CDS282..4793
    Translation

    Target ORF information:

    RefSeq Version XM_018094345.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA
    AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA
    ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA
    GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC
    AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC
    ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA
    GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT
    TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG
    CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA
    GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG
    CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA
    GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG
    CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG
    GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC
    CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA
    GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC
    AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT
    GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG
    ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT
    TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA
    AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA
    GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT
    GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT
    TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA
    ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC
    ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT
    TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA
    AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC
    CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA
    TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA
    TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT
    GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA
    TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT
    TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT
    TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT
    TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT
    TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG
    CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT
    GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC
    TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA
    CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC
    TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA
    ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT
    ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC
    CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA
    GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA
    GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG
    TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC
    GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT
    GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT
    TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC
    ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA
    TATTTCCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10378
    Clone ID Related Accession (Same CDS sequence) XM_004914504.3
    Accession Version XM_004914504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA
    TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT
    GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC
    ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC
    ACAAAACAGC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG
    CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT
    TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA
    GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT
    AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG
    GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT
    GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTTTCCAGG
    ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC
    AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG
    GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG
    AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA
    TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG
    AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT
    GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA
    CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC
    AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA
    TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914561.1
    CDS284..4471
    Translation

    Target ORF information:

    RefSeq Version XM_004914504.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA
    CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG
    CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC
    ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG
    GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT
    AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT
    AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA
    AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC
    CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT
    TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT
    CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG
    CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT
    GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT
    TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA
    TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC
    AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG
    TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG
    GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA
    AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA
    GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA
    CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT
    GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT
    ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT
    GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT
    TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG
    ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA
    TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT
    GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC
    ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC
    ACAAAACAGC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG
    CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT
    TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA
    GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT
    AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG
    GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT
    GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTTTCCAGG
    ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC
    AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG
    GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG
    AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA
    TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG
    AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT
    GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA
    CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC
    AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA
    TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10379
    Clone ID Related Accession (Same CDS sequence) XM_004914505.3
    Accession Version XM_004914505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC
    AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT
    CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG
    GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC
    AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT
    CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT
    GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC
    AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA
    TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT
    GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA
    CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT
    TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG
    TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG
    GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA
    GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG
    TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA
    GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT
    GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC
    GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT
    TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT
    GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT
    GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC
    TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT
    GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA
    ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT
    GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA
    AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC
    GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA
    TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAGC CGGGTGGTGC AACATCTTCT
    TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG
    GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC
    TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG
    ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC
    ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT
    GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT
    ATCCAAATTT ACAGTCTGTC AGTTTCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT
    TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT
    ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT
    AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG
    TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC
    CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT
    CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT
    TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA
    GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA
    GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT
    CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914562.1
    CDS284..4441
    Translation

    Target ORF information:

    RefSeq Version XM_004914505.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT
    CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA
    AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT
    CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG
    GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT
    GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG
    CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG
    TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC
    AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT
    TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT
    TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA
    GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC
    AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT
    CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG
    GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC
    AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT
    CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT
    GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC
    AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA
    TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT
    GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA
    CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT
    TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG
    TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG
    GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA
    GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG
    TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA
    GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT
    GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC
    GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT
    TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT
    GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT
    GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC
    TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT
    GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA
    ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT
    GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA
    AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC
    GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA
    TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAGC CGGGTGGTGC AACATCTTCT
    TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG
    GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC
    TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG
    ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC
    ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT
    GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT
    ATCCAAATTT ACAGTCTGTC AGTTTCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT
    TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT
    ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT
    AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG
    TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC
    CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT
    CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT
    TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA
    GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA
    GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT
    CTCCGATATT TCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10380
    Clone ID Related Accession (Same CDS sequence) XM_004914506.3
    Accession Version XM_004914506.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein FAM135A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT
    ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT
    GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG
    AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT
    GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG
    GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC
    ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG
    TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT
    ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT
    ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA
    GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA
    ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC
    AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC
    ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA
    AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC
    AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC
    ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC
    ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA
    ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT
    GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT
    AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT
    AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT
    ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA
    AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA
    GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT
    GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG
    CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC
    TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG
    ATTGACACTA TCACAAAACA GCCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA
    AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG
    AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT
    AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT
    CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT
    GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT
    GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG
    TCAGTTTCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT
    CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT
    GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT
    ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT
    CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT
    GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA
    ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA
    AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT
    CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG
    GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914563.1
    CDS284..4363
    Translation

    Target ORF information:

    RefSeq Version XM_004914506.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914506.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
    GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT
    CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA
    GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT
    ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA
    GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT
    TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC
    TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC
    TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC
    TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG
    TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT
    GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT
    TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA
    GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT
    CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA
    CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT
    CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA
    TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC
    CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC
    TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC
    TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA
    GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG
    CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT
    GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT
    ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT
    GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG
    AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT
    GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG
    GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC
    ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG
    TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT
    ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT
    ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA
    GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA
    ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC
    AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC
    ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA
    AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC
    AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC
    ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC
    ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA
    ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT
    GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT
    AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT
    AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT
    ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA
    AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA
    GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT
    GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG
    CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC
    TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG
    ATTGACACTA TCACAAAACA GCCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA
    AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG
    AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT
    AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT
    CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT
    GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT
    GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG
    TCAGTTTCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT
    CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT
    GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT
    ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT
    CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT
    GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA
    ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA
    AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT
    CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG
    GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.