fam135a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004914503.3	XP_004914560.1	protein FAM135A isoform X1
XM_018094346.1	XP_017949835.1	protein FAM135A isoform X5
XM_004914504.4	XP_004914561.1	protein FAM135A isoform X3
XM_018094346.2	XP_017949835.1	protein FAM135A isoform X6
XM_018094345.1	XP_017949834.1	protein FAM135A isoform X1
XM_004914505.4	XP_004914562.1	protein FAM135A isoform X4
XM_004914506.4	XP_004914563.1	protein FAM135A isoform X5
XM_004914504.3	XP_004914561.1	protein FAM135A isoform X2
XM_031902991.1	XP_031758851.1	protein FAM135A isoform X1
XM_004914503.4	XP_004914560.1	protein FAM135A isoform X2
XM_004914506.3	XP_004914563.1	protein FAM135A isoform X4
XM_031902992.1	XP_031758852.1	protein FAM135A isoform X1
XM_004914505.3	XP_004914562.1	protein FAM135A isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44720	XM_018094346.1 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa59289	XM_004914504.4 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa59290	XM_004914505.4 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa59291	XM_004914506.4 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa59292	XM_018094346.2 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa59287	XM_031902991.1 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa59287	XM_031902992.1 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa59288	XM_004914503.4 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10377	XM_018094345.1 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10377	XM_004914503.3 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10378	XM_004914504.3 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa10379	XM_004914505.3 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa10380	XM_004914506.3 Latest version!	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

fam135a ( XM_018094346.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949835.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa44720
Clone ID Related Accession (Same CDS sequence)	XM_018094346.1
Accession Version	XM_018094346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA 
TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA 
AAACAGCCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT 
TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC 
TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT 
GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC 
GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA 
TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT 
CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT TTCCAGGATC 
AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA 
TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT 
ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA 
AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC 
TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC 
TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT 
CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT 
GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT 
ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT 
TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949835.1
CDS	284..4348
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_018094346.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094346.1

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA 
TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA 
AAACAGCCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT 
TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC 
TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT 
GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC 
GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA 
TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT 
CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT TTCCAGGATC 
AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA 
TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT 
ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA 
AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC 
TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC 
TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT 
CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT 
GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT 
ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT 
TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914504.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914561.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa59289
Clone ID Related Accession (Same CDS sequence)	XM_004914504.4
Accession Version	XM_004914504.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA 
TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT 
GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC 
ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC 
ACAAAACAAC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG 
CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT 
TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA 
GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT 
AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG 
GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT 
GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTATCCAGG 
ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC 
AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG 
GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG 
AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA 
TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG 
AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT 
GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA 
CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC 
AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA 
TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914561.1
CDS	279..4466
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914504.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914504.4

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA 
TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT 
GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC 
ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC 
ACAAAACAAC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG 
CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT 
TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA 
GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT 
AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG 
GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT 
GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTATCCAGG 
ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC 
AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG 
GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG 
AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA 
TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG 
AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT 
GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA 
CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC 
AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA 
TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914505.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914562.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

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CloneID	OXa59290
Clone ID Related Accession (Same CDS sequence)	XM_004914505.4
Accession Version	XM_004914505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC 
AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT 
CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG 
GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC 
AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT 
CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT 
GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC 
AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA 
TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT 
GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA 
CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT 
TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG 
TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG 
GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA 
GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG 
TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA 
GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT 
GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC 
GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT 
TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT 
GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT 
GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC 
TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT 
GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA 
ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT 
GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA 
AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC 
GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA 
TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAAC CGGGTGGTGC AACATCTTCT 
TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG 
GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC 
TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG 
ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC 
ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT 
GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT 
ATCCAAATTT ACAGTCTGTC AGTATCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT 
TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT 
ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT 
AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG 
TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC 
CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT 
CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT 
TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA 
GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA 
GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT 
CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914562.1
CDS	279..4436
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914505.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914505.4

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC 
AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT 
CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG 
GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC 
AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT 
CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT 
GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC 
AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA 
TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT 
GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA 
CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT 
TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG 
TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG 
GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA 
GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG 
TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA 
GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT 
GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC 
GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT 
TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT 
GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT 
GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC 
TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT 
GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA 
ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT 
GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA 
AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC 
GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA 
TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAAC CGGGTGGTGC AACATCTTCT 
TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG 
GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC 
TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG 
ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC 
ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT 
GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT 
ATCCAAATTT ACAGTCTGTC AGTATCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT 
TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT 
ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT 
AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG 
TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC 
CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT 
CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT 
TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA 
GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA 
GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT 
CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914506.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914563.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

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CloneID	OXa59291
Clone ID Related Accession (Same CDS sequence)	XM_004914506.4
Accession Version	XM_004914506.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914506.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT 
ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT 
GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG 
AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT 
GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG 
GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC 
ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG 
TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT 
ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT 
ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA 
GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA 
ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC 
AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC 
ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA 
AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC 
AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC 
ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC 
ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA 
ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT 
GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT 
AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT 
AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT 
ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA 
AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA 
GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT 
GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG 
CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC 
TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG 
ATTGACACTA TCACAAAACA ACCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA 
AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG 
AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT 
AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT 
CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT 
GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT 
GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG 
TCAGTATCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT 
CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT 
GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT 
ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT 
CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT 
GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA 
ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA 
AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT 
CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG 
GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914563.1
CDS	279..4358
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELEKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914506.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914506.4

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT 
ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT 
GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG 
AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT 
GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG 
GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC 
ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG 
TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT 
ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT 
ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA 
GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA 
ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC 
AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC 
ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA 
AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC 
AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC 
ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC 
ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA 
ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT 
GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT 
AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT 
AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT 
ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA 
AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA 
GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT 
GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG 
CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC 
TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG 
ATTGACACTA TCACAAAACA ACCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA 
AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG 
AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT 
AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT 
CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT 
GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT 
GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG 
TCAGTATCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT 
CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT 
GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT 
ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT 
CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT 
GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA 
ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA 
AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT 
CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG 
GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_018094346.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949835.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.

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CloneID	OXa59292
Clone ID Related Accession (Same CDS sequence)	XM_018094346.2
Accession Version	XM_018094346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA 
TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA 
AAACAACCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT 
TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC 
TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT 
GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC 
GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA 
TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT 
CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT ATCCAGGATC 
AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA 
TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT 
ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA 
AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC 
TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC 
TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT 
CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT 
GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT 
ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT 
TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949835.1
CDS	279..4343
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_018094346.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094346.2

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGCTGTTT TCCCTACACA 
TTGAGAGAAT CTCCTTCTGG TACAGCATTT CCTTCCAGGA ACATGATTGA CACTATCACA 
AAACAACCGG GTGGTGCAAC ATCTTCTTCC CTTTCCTGGT ATGAAAGTTC TCCCAAGCCT 
TTAATGCTGG CATTTCTGCA GGCCAAGGAA GAGCTGAAGC AGTTGAGACT TCCTGGTTTC 
TTATACAGTG ATGTTCCTCA GTTGGCCTCC AGTGTTCCAT ATTTTAGCAT GGAGGAAGAT 
GATGACCCTG ATGAAGGAAT ACATCTGATT GTCTGTGTGC ATGGTCTTGA CGGGAATAGC 
GCTGACCTTC GCCTTGTAAG AACTTACATT GAACTTGGAC TGCCTGGTGG GAGGAAGGAA 
TTCCTTATGT CTGAAAGAAA CCAGAATGAT ACCTTTGCTG ATTTTGACTC CATGACTGAT 
CGGCTTTTAG ATGAAATAGT TCAATATATC CAAATTTACA GTCTGTCAGT ATCCAGGATC 
AGTTTTATAG GCCATTCCTT AGGCAATTTA ATTATCCGTT CAGTTCTTTC AAGACCCAGA 
TTTAAATGTT ACCTCAGCAA GCTGCATACC TTCCTTTCTT TATCTGGGCC ACATCTGGGT 
ACTCTCTACA ATAGCAGTGC TTTGGTTAAT ACAGGCTTAT GGTTTATGCA GAAGTGGAAA 
AAGTCAGGAT CTCTGTTGCA GTTAACGTGC AGAGACAACT CAGATCCCCG ACAAACATTC 
TTGTACAAAC TCAGCAAGAA ACCAGGCCTG GAGTATTTTA GGAATGTGGT TCTAGTGAGC 
TCCTTGCAGG ATCGGTATGT GCCCTATCAC TCCGCTCGCA TTGAAATGTG CAAAACTGCT 
CTGAAGGATA AACAGTCAGG GCCAGTTTAT GCTGAGATGA TCCAAAACAT CCTTTCACCT 
GTTCTACAGA ATAAGGACTG CAATTTAGTG CGTTATGATG TCATTCATGC TTTACCCAAT 
ACAGCCAATT CTCTTATTGG ACGAGCAGCC CACATTGCGG TTTTGGACTC TGAAGTATTT 
TTGGAAAAGT TCTTTCTTGT TGCTGGTCTC CGATATTTCC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_031902991.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758851.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59287
Clone ID Related Accession (Same CDS sequence)	XM_031902991.1 , XM_031902992.1
Accession Version	XM_031902991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA 
AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT 
AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG 
TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG 
GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC 
TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG 
AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT 
TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG 
CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA 
AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT 
CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG 
CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT 
AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA 
AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT 
GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT 
GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA 
GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA 
CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG 
AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT 
TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG 
GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC 
TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT 
TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC 
AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT 
GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA 
CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA 
TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC 
CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT 
GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC 
GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA 
GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT 
GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG 
TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA 
ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT 
TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT 
GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT 
CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT 
CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA 
GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT 
GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA 
AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA 
ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT 
TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC 
AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC 
AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG 
TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC 
AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG 
TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG 
TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG 
GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT 
ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT 
CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758851.1
CDS	280..4869
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGKNEDETKSKTKVNKLMKSMKHENPKKIVKQNSKESVVLVSYKCLKAATAESMSKPFEGRPSHCHKDGRNHMQGFLPSDTKSSGLQTLQKGHTSLSSNAGTNKPLLATFDSTQRNQSSTWNTGNLTPFSASAILGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_031902991.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902991.1

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA 
AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT 
AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG 
TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG 
GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC 
TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG 
AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT 
TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG 
CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA 
AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT 
CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG 
CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT 
AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA 
AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT 
GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT 
GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA 
GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA 
CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG 
AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT 
TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG 
GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC 
TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT 
TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC 
AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT 
GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA 
CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA 
TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC 
CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT 
GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC 
GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA 
GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT 
GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG 
TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA 
ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT 
TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT 
GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT 
CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT 
CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA 
GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT 
GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA 
AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA 
ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT 
TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC 
AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC 
AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG 
TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC 
AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG 
TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG 
TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG 
GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT 
ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT 
CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_031902992.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758852.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

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CloneID	OXa59287
Clone ID Related Accession (Same CDS sequence)	XM_031902991.1 , XM_031902992.1
Accession Version	XM_031902992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA 
AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT 
AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG 
TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG 
GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC 
TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG 
AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT 
TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG 
CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA 
AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT 
CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG 
CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT 
AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA 
AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT 
GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT 
GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA 
GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA 
CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG 
AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT 
TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG 
GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC 
TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT 
TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC 
AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT 
GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA 
CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA 
TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC 
CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT 
GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC 
GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA 
GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT 
GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG 
TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA 
ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT 
TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT 
GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT 
CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT 
CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA 
GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT 
GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA 
AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA 
ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT 
TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC 
AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC 
AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG 
TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC 
AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG 
TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG 
TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG 
GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT 
ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT 
CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758852.1
CDS	277..4866
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGKNEDETKSKTKVNKLMKSMKHENPKKIVKQNSKESVVLVSYKCLKAATAESMSKPFEGRPSHCHKDGRNHMQGFLPSDTKSSGLQTLQKGHTSLSSNAGTNKPLLATFDSTQRNQSSTWNTGNLTPFSASAILGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_031902992.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902992.1

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTAAAAAT GAAGATGAAA CTAAATCAAA AACTAAAGTA 
AATAAACTCA TGAAATCAAT GAAACATGAG AATCCAAAAA AGATAGTAAA ACAAAATTCT 
AAGGAGTCAG TGGTATTAGT GAGCTACAAG TGTTTAAAGG CTGCTACAGC AGAGTCTATG 
TCTAAACCCT TTGAAGGCAG GCCTTCACAT TGTCATAAGG ATGGACGTAA CCACATGCAG 
GGCTTTCTGC CAAGTGACAC TAAGTCCTCT GGGTTACAAA CATTACAAAA AGGGCATACC 
TCTCTGTCAT CTAATGCAGG CACAAATAAA CCACTGTTGG CAACATTTGA CAGTACCCAG 
AGAAACCAAA GCAGTACTTG GAATACTGGT AATCTAACAC CTTTCTCCGC TTCCGCCATT 
TTAGGTACAC TCAGCATGCA ACAAGCAGGT ACTGGGCTTA TGCCACTGGA TAGCGCTCTG 
CAGACTGTTG TTCCTGAAGA AAGCTTCCAT GGTAATCAAA CCATGATCCC TTCAGGAGTA 
AGATCAATAG TGGTTAAGCC ATGTAACAAG AACCCATTTG AGGGTGAGAA GGTGACTGTT 
CTTTTGGGTT CCAAGCCAGA AATGTGTAAT GATCGTATAA CTAAAGATGG ATTGCAGGAG 
CAAGGGAATG CTCCCTTGCA TGGTGGAATG GCATCATCAC TTAATATGGA CACCACATTT 
AATAAAAATG TTGCACTGGA TACAAATTTC ACACTCACTG GAAATCCCGA GAGCTTTTTA 
AAGGTAAAAA GCAATCTCCA AACCACTATG TCAAAAGAGG TCCACATTGT TGTAAGCAGT 
GACACCATTA AATTACCAGA TATTAATCTT ACATGTGCTT CTTCTCGGTT TTCTGATTCT 
GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT ACACATCCAA ATCCAGAACC GAATGTGGAA 
GATCAAATGG CACCAGGTGC AGTTGTGACA GATAAAGTAT TGCCACATTT GTTGCTAAAA 
CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA ACCCACTGCA CTGAGAGTAC CAGTGCACTG 
AGTGAAATTC AGTCATCTTT GACATCAATC AACTCACTGC CTTCAGATGA TGATGAGCTT 
TCGCCAGACG AGGCTTCAAA AATATCTGTC ACGGAAAGTA AATTAAAAGA CAGTAAAACG 
GTTTTAGATT TAGGTACAGT TGACTTGCCA AAGACTGACA GCAAGAAATC TAGCATTCTC 
TTGCAACCTC AGTGTGTTGT TTTCTCTGAC AATGAAACTG TGTCAGTACA TTCTTCCCTT 
TCAAGTATCA GAGATCTTTT TCAGTTTGCC ATTTCAGATG AGGAAACTCC AAATGAAGTC 
AAAAGTATTC CTGTCCCAAT GTCAAACAGC ATAACCACTT TAAGAGAGCT TCAAACCCAT 
GACAAGCATC TCGGTGCCAC CCAAGGCACA ATGAATACAG AAGTTATTGT TTTTGGAGAA 
CGTTCTATAA TTTCAGGTTC TGTTGCCTGT GATAACAGGC CACAACAAGG CATACTAGAA 
TATGGTGAAA CTGGACTAAA TCAGACTAGT AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC 
CAAACTGGAA CTGCAAACCT TTCCTCTAAT AGCAGCTCTG ATATGGTGAA GCAAGGCCTT 
GTTGAAAATT ACTTTGGATC TCAAAGTAGT ACTGACATTT CTGACATTAG CCCAGCCGAC 
GATGTAAAAT ATGCTGCATC TGCAAAAGAA AATGTAAAGC ATTTGCTAGA AGGCCCGCCA 
GTTTCAGAAG AAATTGATGA GGAGGAACAA GATGAAGATA TGATTGAGAA TGGCTACTAT 
GAGGAAACAG ATGGCCCATT ATATAGGTCT GATGATACAG TATGTGAAGT TGAATTTGAG 
TCTGCCAATG AAAAATGTTT AAGATCAGAG CAAATAAGCT CTGAGTATTT ACGAGATTCA 
ATGAACATGC CCGGTGTTTG TACTCCTAGC TGTTTTCCCT ACACATTGAG AGAATCTCCT 
TCTGGTACAG CATTTCCTTC CAGGAACATG ATTGACACTA TCACAAAACA ACCGGGTGGT 
GCAACATCTT CTTCCCTTTC CTGGTATGAA AGTTCTCCCA AGCCTTTAAT GCTGGCATTT 
CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG AGACTTCCTG GTTTCTTATA CAGTGATGTT 
CCTCAGTTGG CCTCCAGTGT TCCATATTTT AGCATGGAGG AAGATGATGA CCCTGATGAA 
GGAATACATC TGATTGTCTG TGTGCATGGT CTTGACGGGA ATAGCGCTGA CCTTCGCCTT 
GTAAGAACTT ACATTGAACT TGGACTGCCT GGTGGGAGGA AGGAATTCCT TATGTCTGAA 
AGAAACCAGA ATGATACCTT TGCTGATTTT GACTCCATGA CTGATCGGCT TTTAGATGAA 
ATAGTTCAAT ATATCCAAAT TTACAGTCTG TCAGTATCCA GGATCAGTTT TATAGGCCAT 
TCCTTAGGCA ATTTAATTAT CCGTTCAGTT CTTTCAAGAC CCAGATTTAA ATGTTACCTC 
AGCAAGCTGC ATACCTTCCT TTCTTTATCT GGGCCACATC TGGGTACTCT CTACAATAGC 
AGTGCTTTGG TTAATACAGG CTTATGGTTT ATGCAGAAGT GGAAAAAGTC AGGATCTCTG 
TTGCAGTTAA CGTGCAGAGA CAACTCAGAT CCCCGACAAA CATTCTTGTA CAAACTCAGC 
AAGAAACCAG GCCTGGAGTA TTTTAGGAAT GTGGTTCTAG TGAGCTCCTT GCAGGATCGG 
TATGTGCCCT ATCACTCCGC TCGCATTGAA ATGTGCAAAA CTGCTCTGAA GGATAAACAG 
TCAGGGCCAG TTTATGCTGA GATGATCCAA AACATCCTTT CACCTGTTCT ACAGAATAAG 
GACTGCAATT TAGTGCGTTA TGATGTCATT CATGCTTTAC CCAATACAGC CAATTCTCTT 
ATTGGACGAG CAGCCCACAT TGCGGTTTTG GACTCTGAAG TATTTTTGGA AAAGTTCTTT 
CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914503.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914560.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

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CloneID	OXa59288
Clone ID Related Accession (Same CDS sequence)	XM_004914503.4
Accession Version	XM_004914503.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914503.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAACCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTATC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914560.1
CDS	280..4791
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELEKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGKNEDETKSKTKVNKLMKSMKHENPKKIVKQNSKESVVLVSYKCLKAATAESMSKPFEGRPSHCHKDGRNHMQGFLPSDTKSSGLQTLQKGHTSLSSNAGTNKPLLATFDSTQRNQSSTWNTGNLTPFSASAILGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914503.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914503.4

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAACCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTATC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914503.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914560.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10377
Clone ID Related Accession (Same CDS sequence)	XM_004914503.3 , XM_018094345.1
Accession Version	XM_004914503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914560.1
CDS	285..4796
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELEKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGKNEDETKSKTKVNKLMKSMKHENPKKIVKQNSKESVVLVSYKCLKAATAESMSKPFEGRPSHCHKDGRNHMQGFLPSDTKSSGLQTLQKGHTSLSSNAGTNKPLLATFDSTQRNQSSTWNTGNLTPFSASAILGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914503.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914503.3

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_018094345.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949834.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10377
Clone ID Related Accession (Same CDS sequence)	XM_004914503.3 , XM_018094345.1
Accession Version	XM_018094345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949834.1
CDS	282..4793
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELEKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGKNEDETKSKTKVNKLMKSMKHENPKKIVKQNSKESVVLVSYKCLKAATAESMSKPFEGRPSHCHKDGRNHMQGFLPSDTKSSGLQTLQKGHTSLSSNAGTNKPLLATFDSTQRNQSSTWNTGNLTPFSASAILGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_018094345.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094345.1

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTGATA AACAGCTTGC CAAGTCCCAA 
AAGGGTAAAA ATGAAGATGA AACTAAATCA AAAACTAAAG TAAATAAACT CATGAAATCA 
ATGAAACATG AGAATCCAAA AAAGATAGTA AAACAAAATT CTAAGGAGTC AGTGGTATTA 
GTGAGCTACA AGTGTTTAAA GGCTGCTACA GCAGAGTCTA TGTCTAAACC CTTTGAAGGC 
AGGCCTTCAC ATTGTCATAA GGATGGACGT AACCACATGC AGGGCTTTCT GCCAAGTGAC 
ACTAAGTCCT CTGGGTTACA AACATTACAA AAAGGGCATA CCTCTCTGTC ATCTAATGCA 
GGCACAAATA AACCACTGTT GGCAACATTT GACAGTACCC AGAGAAACCA AAGCAGTACT 
TGGAATACTG GTAATCTAAC ACCTTTCTCC GCTTCCGCCA TTTTAGGTAC ACTCAGCATG 
CAACAAGCAG GTACTGGGCT TATGCCACTG GATAGCGCTC TGCAGACTGT TGTTCCTGAA 
GAAAGCTTCC ATGGTAATCA AACCATGATC CCTTCAGGAG TAAGATCAAT AGTGGTTAAG 
CCATGTAACA AGAACCCATT TGAGGGTGAG AAGGTGACTG TTCTTTTGGG TTCCAAGCCA 
GAAATGTGTA ATGATCGTAT AACTAAAGAT GGATTGCAGG AGCAAGGGAA TGCTCCCTTG 
CATGGTGGAA TGGCATCATC ACTTAATATG GACACCACAT TTAATAAAAA TGTTGCACTG 
GATACAAATT TCACACTCAC TGGAAATCCC GAGAGCTTTT TAAAGGTAAA AAGCAATCTC 
CAAACCACTA TGTCAAAAGA GGTCCACATT GTTGTAAGCA GTGACACCAT TAAATTACCA 
GATATTAATC TTACATGTGC TTCTTCTCGG TTTTCTGATT CTGGTGTAGA AAGTGAGCCC 
AGCTCTTTTT CTACACATCC AAATCCAGAA CCGAATGTGG AAGATCAAAT GGCACCAGGT 
GCAGTTGTGA CAGATAAAGT ATTGCCACAT TTGTTGCTAA AACCAGAGTC AAATTGTAAG 
ACTGCAGTAG AAACCCACTG CACTGAGAGT ACCAGTGCAC TGAGTGAAAT TCAGTCATCT 
TTGACATCAA TCAACTCACT GCCTTCAGAT GATGATGAGC TTTCGCCAGA CGAGGCTTCA 
AAAATATCTG TCACGGAAAG TAAATTAAAA GACAGTAAAA CGGTTTTAGA TTTAGGTACA 
GTTGACTTGC CAAAGACTGA CAGCAAGAAA TCTAGCATTC TCTTGCAACC TCAGTGTGTT 
GTTTTCTCTG ACAATGAAAC TGTGTCAGTA CATTCTTCCC TTTCAAGTAT CAGAGATCTT 
TTTCAGTTTG CCATTTCAGA TGAGGAAACT CCAAATGAAG TCAAAAGTAT TCCTGTCCCA 
ATGTCAAACA GCATAACCAC TTTAAGAGAG CTTCAAACCC ATGACAAGCA TCTCGGTGCC 
ACCCAAGGCA CAATGAATAC AGAAGTTATT GTTTTTGGAG AACGTTCTAT AATTTCAGGT 
TCTGTTGCCT GTGATAACAG GCCACAACAA GGCATACTAG AATATGGTGA AACTGGACTA 
AATCAGACTA GTAAAGGCTC ATCTGCAGTA ACAGGTGAAA ACCAAACTGG AACTGCAAAC 
CTTTCCTCTA ATAGCAGCTC TGATATGGTG AAGCAAGGCC TTGTTGAAAA TTACTTTGGA 
TCTCAAAGTA GTACTGACAT TTCTGACATT AGCCCAGCCG ACGATGTAAA ATATGCTGCA 
TCTGCAAAAG AAAATGTAAA GCATTTGCTA GAAGGCCCGC CAGTTTCAGA AGAAATTGAT 
GAGGAGGAAC AAGATGAAGA TATGATTGAG AATGGCTACT ATGAGGAAAC AGATGGCCCA 
TTATATAGGT CTGATGATAC AGTATGTGAA GTTGAATTTG AGTCTGCCAA TGAAAAATGT 
TTAAGATCAG AGCAAATAAG CTCTGAGTAT TTACGAGATT CAATGAACAT GCCCGGTGTT 
TGTACTCCTA GCTGTTTTCC CTACACATTG AGAGAATCTC CTTCTGGTAC AGCATTTCCT 
TCCAGGAACA TGATTGACAC TATCACAAAA CAGCCGGGTG GTGCAACATC TTCTTCCCTT 
TCCTGGTATG AAAGTTCTCC CAAGCCTTTA ATGCTGGCAT TTCTGCAGGC CAAGGAAGAG 
CTGAAGCAGT TGAGACTTCC TGGTTTCTTA TACAGTGATG TTCCTCAGTT GGCCTCCAGT 
GTTCCATATT TTAGCATGGA GGAAGATGAT GACCCTGATG AAGGAATACA TCTGATTGTC 
TGTGTGCATG GTCTTGACGG GAATAGCGCT GACCTTCGCC TTGTAAGAAC TTACATTGAA 
CTTGGACTGC CTGGTGGGAG GAAGGAATTC CTTATGTCTG AAAGAAACCA GAATGATACC 
TTTGCTGATT TTGACTCCAT GACTGATCGG CTTTTAGATG AAATAGTTCA ATATATCCAA 
ATTTACAGTC TGTCAGTTTC CAGGATCAGT TTTATAGGCC ATTCCTTAGG CAATTTAATT 
ATCCGTTCAG TTCTTTCAAG ACCCAGATTT AAATGTTACC TCAGCAAGCT GCATACCTTC 
CTTTCTTTAT CTGGGCCACA TCTGGGTACT CTCTACAATA GCAGTGCTTT GGTTAATACA 
GGCTTATGGT TTATGCAGAA GTGGAAAAAG TCAGGATCTC TGTTGCAGTT AACGTGCAGA 
GACAACTCAG ATCCCCGACA AACATTCTTG TACAAACTCA GCAAGAAACC AGGCCTGGAG 
TATTTTAGGA ATGTGGTTCT AGTGAGCTCC TTGCAGGATC GGTATGTGCC CTATCACTCC 
GCTCGCATTG AAATGTGCAA AACTGCTCTG AAGGATAAAC AGTCAGGGCC AGTTTATGCT 
GAGATGATCC AAAACATCCT TTCACCTGTT CTACAGAATA AGGACTGCAA TTTAGTGCGT 
TATGATGTCA TTCATGCTTT ACCCAATACA GCCAATTCTC TTATTGGACG AGCAGCCCAC 
ATTGCGGTTT TGGACTCTGA AGTATTTTTG GAAAAGTTCT TTCTTGTTGC TGGTCTCCGA 
TATTTCCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914504.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914561.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

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CloneID	OXa10378
Clone ID Related Accession (Same CDS sequence)	XM_004914504.3
Accession Version	XM_004914504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA 
TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT 
GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC 
ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC 
ACAAAACAGC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG 
CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT 
TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA 
GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT 
AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG 
GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT 
GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTTTCCAGG 
ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC 
AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG 
GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG 
AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA 
TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG 
AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT 
GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA 
CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC 
AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA 
TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914561.1
CDS	284..4471
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGDKQLAKSQKGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914504.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914504.3

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTGATAAA 
CAGCTTGCCA AGTCCCAAAA GGGTACACTC AGCATGCAAC AAGCAGGTAC TGGGCTTATG 
CCACTGGATA GCGCTCTGCA GACTGTTGTT CCTGAAGAAA GCTTCCATGG TAATCAAACC 
ATGATCCCTT CAGGAGTAAG ATCAATAGTG GTTAAGCCAT GTAACAAGAA CCCATTTGAG 
GGTGAGAAGG TGACTGTTCT TTTGGGTTCC AAGCCAGAAA TGTGTAATGA TCGTATAACT 
AAAGATGGAT TGCAGGAGCA AGGGAATGCT CCCTTGCATG GTGGAATGGC ATCATCACTT 
AATATGGACA CCACATTTAA TAAAAATGTT GCACTGGATA CAAATTTCAC ACTCACTGGA 
AATCCCGAGA GCTTTTTAAA GGTAAAAAGC AATCTCCAAA CCACTATGTC AAAAGAGGTC 
CACATTGTTG TAAGCAGTGA CACCATTAAA TTACCAGATA TTAATCTTAC ATGTGCTTCT 
TCTCGGTTTT CTGATTCTGG TGTAGAAAGT GAGCCCAGCT CTTTTTCTAC ACATCCAAAT 
CCAGAACCGA ATGTGGAAGA TCAAATGGCA CCAGGTGCAG TTGTGACAGA TAAAGTATTG 
CCACATTTGT TGCTAAAACC AGAGTCAAAT TGTAAGACTG CAGTAGAAAC CCACTGCACT 
GAGAGTACCA GTGCACTGAG TGAAATTCAG TCATCTTTGA CATCAATCAA CTCACTGCCT 
TCAGATGATG ATGAGCTTTC GCCAGACGAG GCTTCAAAAA TATCTGTCAC GGAAAGTAAA 
TTAAAAGACA GTAAAACGGT TTTAGATTTA GGTACAGTTG ACTTGCCAAA GACTGACAGC 
AAGAAATCTA GCATTCTCTT GCAACCTCAG TGTGTTGTTT TCTCTGACAA TGAAACTGTG 
TCAGTACATT CTTCCCTTTC AAGTATCAGA GATCTTTTTC AGTTTGCCAT TTCAGATGAG 
GAAACTCCAA ATGAAGTCAA AAGTATTCCT GTCCCAATGT CAAACAGCAT AACCACTTTA 
AGAGAGCTTC AAACCCATGA CAAGCATCTC GGTGCCACCC AAGGCACAAT GAATACAGAA 
GTTATTGTTT TTGGAGAACG TTCTATAATT TCAGGTTCTG TTGCCTGTGA TAACAGGCCA 
CAACAAGGCA TACTAGAATA TGGTGAAACT GGACTAAATC AGACTAGTAA AGGCTCATCT 
GCAGTAACAG GTGAAAACCA AACTGGAACT GCAAACCTTT CCTCTAATAG CAGCTCTGAT 
ATGGTGAAGC AAGGCCTTGT TGAAAATTAC TTTGGATCTC AAAGTAGTAC TGACATTTCT 
GACATTAGCC CAGCCGACGA TGTAAAATAT GCTGCATCTG CAAAAGAAAA TGTAAAGCAT 
TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA ATTGATGAGG AGGAACAAGA TGAAGATATG 
ATTGAGAATG GCTACTATGA GGAAACAGAT GGCCCATTAT ATAGGTCTGA TGATACAGTA 
TGTGAAGTTG AATTTGAGTC TGCCAATGAA AAATGTTTAA GATCAGAGCA AATAAGCTCT 
GAGTATTTAC GAGATTCAAT GAACATGCCC GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC 
ACATTGAGAG AATCTCCTTC TGGTACAGCA TTTCCTTCCA GGAACATGAT TGACACTATC 
ACAAAACAGC CGGGTGGTGC AACATCTTCT TCCCTTTCCT GGTATGAAAG TTCTCCCAAG 
CCTTTAATGC TGGCATTTCT GCAGGCCAAG GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT 
TTCTTATACA GTGATGTTCC TCAGTTGGCC TCCAGTGTTC CATATTTTAG CATGGAGGAA 
GATGATGACC CTGATGAAGG AATACATCTG ATTGTCTGTG TGCATGGTCT TGACGGGAAT 
AGCGCTGACC TTCGCCTTGT AAGAACTTAC ATTGAACTTG GACTGCCTGG TGGGAGGAAG 
GAATTCCTTA TGTCTGAAAG AAACCAGAAT GATACCTTTG CTGATTTTGA CTCCATGACT 
GATCGGCTTT TAGATGAAAT AGTTCAATAT ATCCAAATTT ACAGTCTGTC AGTTTCCAGG 
ATCAGTTTTA TAGGCCATTC CTTAGGCAAT TTAATTATCC GTTCAGTTCT TTCAAGACCC 
AGATTTAAAT GTTACCTCAG CAAGCTGCAT ACCTTCCTTT CTTTATCTGG GCCACATCTG 
GGTACTCTCT ACAATAGCAG TGCTTTGGTT AATACAGGCT TATGGTTTAT GCAGAAGTGG 
AAAAAGTCAG GATCTCTGTT GCAGTTAACG TGCAGAGACA ACTCAGATCC CCGACAAACA 
TTCTTGTACA AACTCAGCAA GAAACCAGGC CTGGAGTATT TTAGGAATGT GGTTCTAGTG 
AGCTCCTTGC AGGATCGGTA TGTGCCCTAT CACTCCGCTC GCATTGAAAT GTGCAAAACT 
GCTCTGAAGG ATAAACAGTC AGGGCCAGTT TATGCTGAGA TGATCCAAAA CATCCTTTCA 
CCTGTTCTAC AGAATAAGGA CTGCAATTTA GTGCGTTATG ATGTCATTCA TGCTTTACCC 
AATACAGCCA ATTCTCTTAT TGGACGAGCA GCCCACATTG CGGTTTTGGA CTCTGAAGTA 
TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914505.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914562.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

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CloneID	OXa10379
Clone ID Related Accession (Same CDS sequence)	XM_004914505.3
Accession Version	XM_004914505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC 
AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT 
CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG 
GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC 
AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT 
CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT 
GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC 
AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA 
TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT 
GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA 
CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT 
TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG 
TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG 
GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA 
GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG 
TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA 
GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT 
GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC 
GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT 
TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT 
GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT 
GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC 
TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT 
GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA 
ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT 
GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA 
AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC 
GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA 
TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAGC CGGGTGGTGC AACATCTTCT 
TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG 
GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC 
TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG 
ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC 
ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT 
GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT 
ATCCAAATTT ACAGTCTGTC AGTTTCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT 
TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT 
ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT 
AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG 
TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC 
CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT 
CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT 
TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA 
GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA 
GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT 
CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914562.1
CDS	284..4441
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELAKSRMQVLYDRLFRRKHSSGQRDSTREKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914505.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914505.3

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG CCAAGTCCCG CATGCAGGTT 
CTTTATGACC GTCTCTTCAG AAGAAAACAT TCATCGGGCC AGCGAGATTC TACTCGGGAA 
AAAATTGATG TTGACGCTCG CCTCACAGAA TTATGTGAAG AAGTTAAGAA AATAGAAAAT 
CCTGATGAAC TGGCAGAACT GATAAACATG AACCTTGCAC AGCTTTGCTC TCTTCTTATG 
GCTTTGTGGG GACAGTTTCT TGAAGTTGTT GTGTTACAAG ATGAAATCAC AACCTTTATT 
GCACAACAGC ACCATATATT ACGGGTGCGT AGGTTTGCTG AGGCTTTCTT TTGTCTTGAG 
CACCCTCGAC AAGCAGCCCT AGCATATCAG GAACTGCATG CCCAAAGTCA TCTTCAGATG 
TCCACAGCTA TAAAAAACTC TTCATACTTT AGTTCTCTCC CTCCACTTCC CATTGAGTGC 
AGTGAACTGG ATGGAGACTT TACCTCTCTG CCTATAATAT TTGAAGATAG GTATTTAGAT 
TATATCATGG AAGATTTAGA TGGACCATGG TTGGGAGCTC AGAACTTACA AAGGTTGGAT 
TCCAGCAAAC AGGAGAAGCA TGACAATGAC GAGGAATCTG TGCGTGGGCC AGTGAGCCCA 
GTGCTTAAAG TCAGGACTGA AGGTGCCTCA ACTCCTGCTT GGTGTACTGA AGGTACACTC 
AGCATGCAAC AAGCAGGTAC TGGGCTTATG CCACTGGATA GCGCTCTGCA GACTGTTGTT 
CCTGAAGAAA GCTTCCATGG TAATCAAACC ATGATCCCTT CAGGAGTAAG ATCAATAGTG 
GTTAAGCCAT GTAACAAGAA CCCATTTGAG GGTGAGAAGG TGACTGTTCT TTTGGGTTCC 
AAGCCAGAAA TGTGTAATGA TCGTATAACT AAAGATGGAT TGCAGGAGCA AGGGAATGCT 
CCCTTGCATG GTGGAATGGC ATCATCACTT AATATGGACA CCACATTTAA TAAAAATGTT 
GCACTGGATA CAAATTTCAC ACTCACTGGA AATCCCGAGA GCTTTTTAAA GGTAAAAAGC 
AATCTCCAAA CCACTATGTC AAAAGAGGTC CACATTGTTG TAAGCAGTGA CACCATTAAA 
TTACCAGATA TTAATCTTAC ATGTGCTTCT TCTCGGTTTT CTGATTCTGG TGTAGAAAGT 
GAGCCCAGCT CTTTTTCTAC ACATCCAAAT CCAGAACCGA ATGTGGAAGA TCAAATGGCA 
CCAGGTGCAG TTGTGACAGA TAAAGTATTG CCACATTTGT TGCTAAAACC AGAGTCAAAT 
TGTAAGACTG CAGTAGAAAC CCACTGCACT GAGAGTACCA GTGCACTGAG TGAAATTCAG 
TCATCTTTGA CATCAATCAA CTCACTGCCT TCAGATGATG ATGAGCTTTC GCCAGACGAG 
GCTTCAAAAA TATCTGTCAC GGAAAGTAAA TTAAAAGACA GTAAAACGGT TTTAGATTTA 
GGTACAGTTG ACTTGCCAAA GACTGACAGC AAGAAATCTA GCATTCTCTT GCAACCTCAG 
TGTGTTGTTT TCTCTGACAA TGAAACTGTG TCAGTACATT CTTCCCTTTC AAGTATCAGA 
GATCTTTTTC AGTTTGCCAT TTCAGATGAG GAAACTCCAA ATGAAGTCAA AAGTATTCCT 
GTCCCAATGT CAAACAGCAT AACCACTTTA AGAGAGCTTC AAACCCATGA CAAGCATCTC 
GGTGCCACCC AAGGCACAAT GAATACAGAA GTTATTGTTT TTGGAGAACG TTCTATAATT 
TCAGGTTCTG TTGCCTGTGA TAACAGGCCA CAACAAGGCA TACTAGAATA TGGTGAAACT 
GGACTAAATC AGACTAGTAA AGGCTCATCT GCAGTAACAG GTGAAAACCA AACTGGAACT 
GCAAACCTTT CCTCTAATAG CAGCTCTGAT ATGGTGAAGC AAGGCCTTGT TGAAAATTAC 
TTTGGATCTC AAAGTAGTAC TGACATTTCT GACATTAGCC CAGCCGACGA TGTAAAATAT 
GCTGCATCTG CAAAAGAAAA TGTAAAGCAT TTGCTAGAAG GCCCGCCAGT TTCAGAAGAA 
ATTGATGAGG AGGAACAAGA TGAAGATATG ATTGAGAATG GCTACTATGA GGAAACAGAT 
GGCCCATTAT ATAGGTCTGA TGATACAGTA TGTGAAGTTG AATTTGAGTC TGCCAATGAA 
AAATGTTTAA GATCAGAGCA AATAAGCTCT GAGTATTTAC GAGATTCAAT GAACATGCCC 
GGTGTTTGTA CTCCTAGCTG TTTTCCCTAC ACATTGAGAG AATCTCCTTC TGGTACAGCA 
TTTCCTTCCA GGAACATGAT TGACACTATC ACAAAACAGC CGGGTGGTGC AACATCTTCT 
TCCCTTTCCT GGTATGAAAG TTCTCCCAAG CCTTTAATGC TGGCATTTCT GCAGGCCAAG 
GAAGAGCTGA AGCAGTTGAG ACTTCCTGGT TTCTTATACA GTGATGTTCC TCAGTTGGCC 
TCCAGTGTTC CATATTTTAG CATGGAGGAA GATGATGACC CTGATGAAGG AATACATCTG 
ATTGTCTGTG TGCATGGTCT TGACGGGAAT AGCGCTGACC TTCGCCTTGT AAGAACTTAC 
ATTGAACTTG GACTGCCTGG TGGGAGGAAG GAATTCCTTA TGTCTGAAAG AAACCAGAAT 
GATACCTTTG CTGATTTTGA CTCCATGACT GATCGGCTTT TAGATGAAAT AGTTCAATAT 
ATCCAAATTT ACAGTCTGTC AGTTTCCAGG ATCAGTTTTA TAGGCCATTC CTTAGGCAAT 
TTAATTATCC GTTCAGTTCT TTCAAGACCC AGATTTAAAT GTTACCTCAG CAAGCTGCAT 
ACCTTCCTTT CTTTATCTGG GCCACATCTG GGTACTCTCT ACAATAGCAG TGCTTTGGTT 
AATACAGGCT TATGGTTTAT GCAGAAGTGG AAAAAGTCAG GATCTCTGTT GCAGTTAACG 
TGCAGAGACA ACTCAGATCC CCGACAAACA TTCTTGTACA AACTCAGCAA GAAACCAGGC 
CTGGAGTATT TTAGGAATGT GGTTCTAGTG AGCTCCTTGC AGGATCGGTA TGTGCCCTAT 
CACTCCGCTC GCATTGAAAT GTGCAAAACT GCTCTGAAGG ATAAACAGTC AGGGCCAGTT 
TATGCTGAGA TGATCCAAAA CATCCTTTCA CCTGTTCTAC AGAATAAGGA CTGCAATTTA 
GTGCGTTATG ATGTCATTCA TGCTTTACCC AATACAGCCA ATTCTCTTAT TGGACGAGCA 
GCCCACATTG CGGTTTTGGA CTCTGAAGTA TTTTTGGAAA AGTTCTTTCT TGTTGCTGGT 
CTCCGATATT TCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_004914506.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914563.1 Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

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CloneID	OXa10380
Clone ID Related Accession (Same CDS sequence)	XM_004914506.3
Accession Version	XM_004914506.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT 
ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT 
GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG 
AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT 
GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG 
GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC 
ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG 
TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT 
ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT 
ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA 
GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA 
ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC 
AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC 
ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA 
AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC 
AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC 
ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC 
ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA 
ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT 
GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT 
AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT 
AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT 
ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA 
AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA 
GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT 
GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG 
CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC 
TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG 
ATTGACACTA TCACAAAACA GCCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA 
AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG 
AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT 
AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT 
CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT 
GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT 
GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG 
TCAGTTTCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT 
CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT 
GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT 
ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT 
CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT 
GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA 
ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA 
AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT 
CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG 
GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914563.1
CDS	284..4363
Translation	MSEVQATVEFSVELQKFYNVDLFQRGFYQIRTSMKIPPRIPHKIEASLLRITGDLAFPASVQDTVVFSKIFQILYKNEEITVNDVMIFKIKMLLDERKIEETLNELNFQMFIDLYFTDTDYSPNDPNALQLISSRSLKLHFNLHRGLHDHINVMFDYFHLSVISVVIHGSLVALHQPLISFHRVIKNTWLNRNAPAQSKESSIPTLESVVFGSSYTKQLSADGNSFVVQDCFLQHAYSFHSTLCACLLFAFKGLDSYFITVSKELPPSLKLELEKIDVDARLTELCEEVKKIENPDELAELINMNLAQLCSLLMALWGQFLEVVVLQDEITTFIAQQHHILRVRRFAEAFFCLEHPRQAALAYQELHAQSHLQMSTAIKNSSYFSSLPPLPIECSELDGDFTSLPIIFEDRYLDYIMEDLDGPWLGAQNLQRLDSSKQEKHDNDEESVRGPVSPVLKVRTEGASTPAWCTEGTLSMQQAGTGLMPLDSALQTVVPEESFHGNQTMIPSGVRSIVVKPCNKNPFEGEKVTVLLGSKPEMCNDRITKDGLQEQGNAPLHGGMASSLNMDTTFNKNVALDTNFTLTGNPESFLKVKSNLQTTMSKEVHIVVSSDTIKLPDINLTCASSRFSDSGVESEPSSFSTHPNPEPNVEDQMAPGAVVTDKVLPHLLLKPESNCKTAVETHCTESTSALSEIQSSLTSINSLPSDDDELSPDEASKISVTESKLKDSKTVLDLGTVDLPKTDSKKSSILLQPQCVVFSDNETVSVHSSLSSIRDLFQFAISDEETPNEVKSIPVPMSNSITTLRELQTHDKHLGATQGTMNTEVIVFGERSIISGSVACDNRPQQGILEYGETGLNQTSKGSSAVTGENQTGTANLSSNSSSDMVKQGLVENYFGSQSSTDISDISPADDVKYAASAKENVKHLLEGPPVSEEIDEEEQDEDMIENGYYEETDGPLYRSDDTVCEVEFESANEKCLRSEQISSEYLRDSMNMPGVCTPSCFPYTLRESPSGTAFPSRNMIDTITKQPGGATSSSLSWYESSPKPLMLAFLQAKEELKQLRLPGFLYSDVPQLASSVPYFSMEEDDDPDEGIHLIVCVHGLDGNSADLRLVRTYIELGLPGGRKEFLMSERNQNDTFADFDSMTDRLLDEIVQYIQIYSLSVSRISFIGHSLGNLIIRSVLSRPRFKCYLSKLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDNSDPRQTFLYKLSKKPGLEYFRNVVLVSSLQDRYVPYHSARIEMCKTALKDKQSGPVYAEMIQNILSPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEVFLEKFFLVAGLRYFQ

Target ORF information:

RefSeq Version	XM_004914506.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914506.3

ORF Insert Sequence:

ATGAGTGAAG TTCAAGCCAC AGTGGAATTT TCTGTGGAGT TGCAAAAATT CTACAATGTG 
GATTTATTTC AAAGAGGTTT TTATCAAATC CGAACATCTA TGAAGATTCC ACCAAGAATT 
CCACACAAAA TTGAAGCAAG CCTTTTACGT ATCACAGGTG ATTTGGCCTT TCCAGCCTCA 
GTCCAAGATA CTGTAGTTTT CAGTAAAATA TTTCAAATCC TTTACAAGAA TGAGGAGATT 
ACGGTTAATG ATGTAATGAT TTTCAAAATT AAAATGCTTC TGGATGAAAG GAAGATTGAA 
GAAACTCTTA ATGAACTGAA TTTCCAAATG TTCATTGACT TATACTTCAC CGACACAGAT 
TATTCACCAA ATGATCCAAA TGCCTTGCAA CTTATTAGCA GCCGGAGCTT AAAGCTGCAC 
TTTAACCTCC ATAGAGGCCT TCATGACCAC ATTAATGTAA TGTTTGATTA CTTCCACCTC 
TCTGTGATAT CTGTTGTTAT TCATGGCTCC TTGGTTGCAT TACATCAACC ATTGATAAGC 
TTTCACCGGG TGATAAAAAA TACATGGTTA AACAGAAATG CACCAGCCCA GAGCAAGGAG 
TCCTCTATTC CAACACTGGA AAGTGTAGTG TTTGGTAGTA GCTACACAAA ACAGTTATCT 
GCGGATGGCA ACAGCTTTGT AGTTCAAGAC TGCTTCTTAC AACATGCGTA CAGTTTCCAT 
TCTACACTGT GTGCCTGCTT GCTGTTTGCT TTCAAAGGAC TGGACAGCTA TTTCATTACA 
GTTTCAAAGG AGCTACCGCC TTCTCTCAAG CTGGAACTTG AAAAAATTGA TGTTGACGCT 
CGCCTCACAG AATTATGTGA AGAAGTTAAG AAAATAGAAA ATCCTGATGA ACTGGCAGAA 
CTGATAAACA TGAACCTTGC ACAGCTTTGC TCTCTTCTTA TGGCTTTGTG GGGACAGTTT 
CTTGAAGTTG TTGTGTTACA AGATGAAATC ACAACCTTTA TTGCACAACA GCACCATATA 
TTACGGGTGC GTAGGTTTGC TGAGGCTTTC TTTTGTCTTG AGCACCCTCG ACAAGCAGCC 
CTAGCATATC AGGAACTGCA TGCCCAAAGT CATCTTCAGA TGTCCACAGC TATAAAAAAC 
TCTTCATACT TTAGTTCTCT CCCTCCACTT CCCATTGAGT GCAGTGAACT GGATGGAGAC 
TTTACCTCTC TGCCTATAAT ATTTGAAGAT AGGTATTTAG ATTATATCAT GGAAGATTTA 
GATGGACCAT GGTTGGGAGC TCAGAACTTA CAAAGGTTGG ATTCCAGCAA ACAGGAGAAG 
CATGACAATG ACGAGGAATC TGTGCGTGGG CCAGTGAGCC CAGTGCTTAA AGTCAGGACT 
GAAGGTGCCT CAACTCCTGC TTGGTGTACT GAAGGTACAC TCAGCATGCA ACAAGCAGGT 
ACTGGGCTTA TGCCACTGGA TAGCGCTCTG CAGACTGTTG TTCCTGAAGA AAGCTTCCAT 
GGTAATCAAA CCATGATCCC TTCAGGAGTA AGATCAATAG TGGTTAAGCC ATGTAACAAG 
AACCCATTTG AGGGTGAGAA GGTGACTGTT CTTTTGGGTT CCAAGCCAGA AATGTGTAAT 
GATCGTATAA CTAAAGATGG ATTGCAGGAG CAAGGGAATG CTCCCTTGCA TGGTGGAATG 
GCATCATCAC TTAATATGGA CACCACATTT AATAAAAATG TTGCACTGGA TACAAATTTC 
ACACTCACTG GAAATCCCGA GAGCTTTTTA AAGGTAAAAA GCAATCTCCA AACCACTATG 
TCAAAAGAGG TCCACATTGT TGTAAGCAGT GACACCATTA AATTACCAGA TATTAATCTT 
ACATGTGCTT CTTCTCGGTT TTCTGATTCT GGTGTAGAAA GTGAGCCCAG CTCTTTTTCT 
ACACATCCAA ATCCAGAACC GAATGTGGAA GATCAAATGG CACCAGGTGC AGTTGTGACA 
GATAAAGTAT TGCCACATTT GTTGCTAAAA CCAGAGTCAA ATTGTAAGAC TGCAGTAGAA 
ACCCACTGCA CTGAGAGTAC CAGTGCACTG AGTGAAATTC AGTCATCTTT GACATCAATC 
AACTCACTGC CTTCAGATGA TGATGAGCTT TCGCCAGACG AGGCTTCAAA AATATCTGTC 
ACGGAAAGTA AATTAAAAGA CAGTAAAACG GTTTTAGATT TAGGTACAGT TGACTTGCCA 
AAGACTGACA GCAAGAAATC TAGCATTCTC TTGCAACCTC AGTGTGTTGT TTTCTCTGAC 
AATGAAACTG TGTCAGTACA TTCTTCCCTT TCAAGTATCA GAGATCTTTT TCAGTTTGCC 
ATTTCAGATG AGGAAACTCC AAATGAAGTC AAAAGTATTC CTGTCCCAAT GTCAAACAGC 
ATAACCACTT TAAGAGAGCT TCAAACCCAT GACAAGCATC TCGGTGCCAC CCAAGGCACA 
ATGAATACAG AAGTTATTGT TTTTGGAGAA CGTTCTATAA TTTCAGGTTC TGTTGCCTGT 
GATAACAGGC CACAACAAGG CATACTAGAA TATGGTGAAA CTGGACTAAA TCAGACTAGT 
AAAGGCTCAT CTGCAGTAAC AGGTGAAAAC CAAACTGGAA CTGCAAACCT TTCCTCTAAT 
AGCAGCTCTG ATATGGTGAA GCAAGGCCTT GTTGAAAATT ACTTTGGATC TCAAAGTAGT 
ACTGACATTT CTGACATTAG CCCAGCCGAC GATGTAAAAT ATGCTGCATC TGCAAAAGAA 
AATGTAAAGC ATTTGCTAGA AGGCCCGCCA GTTTCAGAAG AAATTGATGA GGAGGAACAA 
GATGAAGATA TGATTGAGAA TGGCTACTAT GAGGAAACAG ATGGCCCATT ATATAGGTCT 
GATGATACAG TATGTGAAGT TGAATTTGAG TCTGCCAATG AAAAATGTTT AAGATCAGAG 
CAAATAAGCT CTGAGTATTT ACGAGATTCA ATGAACATGC CCGGTGTTTG TACTCCTAGC 
TGTTTTCCCT ACACATTGAG AGAATCTCCT TCTGGTACAG CATTTCCTTC CAGGAACATG 
ATTGACACTA TCACAAAACA GCCGGGTGGT GCAACATCTT CTTCCCTTTC CTGGTATGAA 
AGTTCTCCCA AGCCTTTAAT GCTGGCATTT CTGCAGGCCA AGGAAGAGCT GAAGCAGTTG 
AGACTTCCTG GTTTCTTATA CAGTGATGTT CCTCAGTTGG CCTCCAGTGT TCCATATTTT 
AGCATGGAGG AAGATGATGA CCCTGATGAA GGAATACATC TGATTGTCTG TGTGCATGGT 
CTTGACGGGA ATAGCGCTGA CCTTCGCCTT GTAAGAACTT ACATTGAACT TGGACTGCCT 
GGTGGGAGGA AGGAATTCCT TATGTCTGAA AGAAACCAGA ATGATACCTT TGCTGATTTT 
GACTCCATGA CTGATCGGCT TTTAGATGAA ATAGTTCAAT ATATCCAAAT TTACAGTCTG 
TCAGTTTCCA GGATCAGTTT TATAGGCCAT TCCTTAGGCA ATTTAATTAT CCGTTCAGTT 
CTTTCAAGAC CCAGATTTAA ATGTTACCTC AGCAAGCTGC ATACCTTCCT TTCTTTATCT 
GGGCCACATC TGGGTACTCT CTACAATAGC AGTGCTTTGG TTAATACAGG CTTATGGTTT 
ATGCAGAAGT GGAAAAAGTC AGGATCTCTG TTGCAGTTAA CGTGCAGAGA CAACTCAGAT 
CCCCGACAAA CATTCTTGTA CAAACTCAGC AAGAAACCAG GCCTGGAGTA TTTTAGGAAT 
GTGGTTCTAG TGAGCTCCTT GCAGGATCGG TATGTGCCCT ATCACTCCGC TCGCATTGAA 
ATGTGCAAAA CTGCTCTGAA GGATAAACAG TCAGGGCCAG TTTATGCTGA GATGATCCAA 
AACATCCTTT CACCTGTTCT ACAGAATAAG GACTGCAATT TAGTGCGTTA TGATGTCATT 
CATGCTTTAC CCAATACAGC CAATTCTCTT ATTGGACGAG CAGCCCACAT TGCGGTTTTG 
GACTCTGAAG TATTTTTGGA AAAGTTCTTT CTTGTTGCTG GTCTCCGATA TTTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	fam135a
Entrez Gene ID	779489
Full Name	family with sequence similarity 135 member A
General protein information	Preferred Names family with sequence similarity 135 member A Names protein FAM135A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	FAM135A	NP_001156001.1
	LOC714862	XP_002803850.1
Gallus gallus (chicken)	FAM135A	XP_419888.2
Danio rerio (zebrafish)	LOC566435	NP_001139066.1
Caenorhabditis elegans (roundworm)	C09D4.4	NP_491607.2
Pan troglodytes (chimpanzee)	FAM135A	XP_001138286.1
Bos taurus (cattle)	FAM135A	XP_002690050.1
Xenopus tropicalis (tropical clawed frog)	fam135a	XP_002939445.1
Canis lupus familiaris (dog)	FAM135A	XP_005627583.1
Mus musculus (house mouse)	Fam135a	NP_080880.4
Rattus norvegicus (Norway rat)	Fam135a	NP_001128069.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

fam135a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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