mki67 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018095816.1	XP_017951305.1	antigen KI-67 isoform X6
XM_031905884.1	XP_031761744.1	proliferation marker protein Ki-67 isoform X1
XM_031905888.1	XP_031761748.1	proliferation marker protein Ki-67 isoform X2
XM_031905893.1	XP_031761753.1	proliferation marker protein Ki-67 isoform X7
XM_031905899.1	XP_031761759.1	proliferation marker protein Ki-67 isoform X12
XM_018095811.1	XP_017951300.1	antigen KI-67 isoform X1
XM_018095818.1	XP_017951307.1	antigen KI-67 isoform X8
XM_031905887.1	XP_031761747.1	proliferation marker protein Ki-67 isoform X1
XM_031905889.1	XP_031761749.1	proliferation marker protein Ki-67 isoform X3
XM_031905890.1	XP_031761750.1	proliferation marker protein Ki-67 isoform X4
XM_031905894.1	XP_031761754.1	proliferation marker protein Ki-67 isoform X8
XM_031905897.1	XP_031761757.1	proliferation marker protein Ki-67 isoform X10
XM_018095810.1	XP_017951299.1	antigen KI-67 isoform X1
XM_018095812.1	XP_017951301.1	antigen KI-67 isoform X2
XM_018095814.1	XP_017951303.1	antigen KI-67 isoform X4
XM_018095815.1	XP_017951304.1	antigen KI-67 isoform X5
XM_018095817.1	XP_017951306.1	antigen KI-67 isoform X7
XM_031905895.1	XP_031761755.1	proliferation marker protein Ki-67 isoform X9
XM_012959628.2	XP_012815082.1	antigen KI-67 isoform X1
XM_018095813.1	XP_017951302.1	antigen KI-67 isoform X3
XM_031905886.1	XP_031761746.1	proliferation marker protein Ki-67 isoform X1
XM_031905891.1	XP_031761751.1	proliferation marker protein Ki-67 isoform X5
XM_031905892.1	XP_031761752.1	proliferation marker protein Ki-67 isoform X6
XM_031905898.1	XP_031761758.1	proliferation marker protein Ki-67 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa28916	XM_012959628.2 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa28916	XM_018095810.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa28916	XM_018095811.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45731	XM_018095812.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45732	XM_018095813.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45733	XM_018095814.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45734	XM_018095815.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45735	XM_018095816.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45736	XM_018095817.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45737	XM_018095818.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62322	XM_031905884.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62322	XM_031905887.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62322	XM_031905886.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62323	XM_031905888.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62324	XM_031905889.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62325	XM_031905891.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62325	XM_031905890.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62326	XM_031905892.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62327	XM_031905893.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62328	XM_031905894.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62329	XM_031905895.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62330	XM_031905897.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62331	XM_031905898.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa62332	XM_031905899.1 Latest version!	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

mki67 ( XM_018095811.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951300.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa28916
Clone ID Related Accession (Same CDS sequence)	XM_018095811.1 , XM_018095810.1 , XM_012959628.2
Accession Version	XM_018095811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951300.1
CDS	135..6848
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095811.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095811.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095810.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951299.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.

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CloneID	OXa28916
Clone ID Related Accession (Same CDS sequence)	XM_018095811.1 , XM_018095810.1 , XM_012959628.2
Accession Version	XM_018095810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951299.1
CDS	123..6836
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095810.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095810.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_012959628.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815082.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.

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CloneID	OXa28916
Clone ID Related Accession (Same CDS sequence)	XM_018095811.1 , XM_018095810.1 , XM_012959628.2
Accession Version	XM_012959628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815082.1
CDS	220..6933
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_012959628.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959628.2

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GTTCCCATAA TTATAGATAA GTCTGTTGAA AAGAATAGTA CACCAGAGCA TGAAAAGAAC 
CTGGAAGAAA GCAATGAAGG AACATCTCTA CAGGTACAGA CCATTGAAGA TGCTTCTTCC 
ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG 
AGTCATTCAC CAGGTTTAGA AAAACCTGTT GTAGATGTTC CAGAAGACCT AAGCACCAGC 
AATGAGGTCT CTAATATTCC TGAAGGATCA AGTCATATCA ATACAGACCA AGAGAACAAT 
GTTGATTTAC CAATGGAAAA AGAGTCTGAA CCGTTAAATC TAAATATTGG AGCGAAAAAA 
GTCACTAAGA AATCTCAGAG AAAGAAAGTC ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA 
AACTTGGTTG TTGAACAAGA TGATGAAAAA TCTGCTCCCC AAAGACGACG AGGAAGACCT 
CAAAGGGGTG GTCTTTCATC TACCACTGAA GTTTTAGACG AAGCATTACC AGAAGAGCCT 
CCAACAAACA GACGAACTGG CAGGGGCCGA AAGATTGCAG AAGTGGCAAA ACCGCAACAA 
GTCAGTACAA GTGTTGAAGA ATCTCAAGAA GTAAGAAACG AGGAACTCTC AGCAACTAAG 
ACTCAGACTA AGTCTACCAG GCATCAAAAA GCTGTATCTG AAGACTCTAC AGTTACAGCA 
ACTAAGAAGC CACGTGGAAG GCAACCTAAA GTGGACAGAG AACAGAAAGC TGCTGAGGAG 
GAAAATACTG TGGAGAAATC ACTTACAGAA CTGCCAGGAG ATTCAGCTTT AGTCAACAAT 
GTAACGCCAT CTGTGAGAAG GGGTAGGCGA GCAAGAGGCA ATGCAAATGC TGTGACTGAA 
ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC TTGACAAACA ATATCGTTTT ACATAAAGAG 
CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA GCCAAAACTC AAAGAGGAAA AAGGAAAAAT 
CTTAAAAATA CAAACGTTGA TTCTGTAAGC AACTCTGCTG AAACAGATGT TGCAACATTA 
GAATTAGAAG AGAGACCTAA CAAAAGATCT ACACGTGGTG TTAGAGGCAC ACAGCAAGTT 
GATGAGCTTA AAGAACCAAA AGGAAAAAAA GCAAAAGTAG AACATGTGCC CAAACCTGCT 
GAACTAGTTC ATATACAGGA GAATCTAGCT AAAGAGAATG CATCTAGGGC GAAATCTGTG 
CAGTGGCACC CTCTTCTGAC TGTTAAGGGG ACACCTGAAA AGAATACTGA AGAGACTGTT 
AAGAATCTTG CAGATGAAGA AGCTCCGGTC AGTGCAGTAA AATCTAGAGG AAGGAGGCGG 
CAAGAAGTAA AAGAATCTGT TCCAGCAAAA CGATCGCGTT CAAGAAGGGA GGAAAATAAT 
GCAGAGGCAT TAACTTCTAG TAGTACAACA GCTGATGCAG TTCCTATTGC TTTGGTTGCC 
AGTGAAAGTA CTGAAACTCC TAAGAATAAG AGAGGACGGA GGAAAGCAAA CACACAATTA 
GAGAAAGTTA CTGATTCAGA TCTTTCAGTG ACAGAGGTTT CCTCTAAGCT AGACCTAGTG 
CCAGAGGTTG TAAACGAAAA GGCCGTAAAC AAACGAAACA CAGGTAAAAG AAACCTCAAG 
TCTTCATCTG ATAGTGATTC TAGTAGTAAA GCTGTTGAAC CAGTGCTGGA GACTATTACC 
CAAAAGCGTG GACGAAGAGG AGCAGCAGCC AAAAGTCTAG ATTCTGAGAC TACTGAAGTT 
AAAACTGTTG AGTCTGCTGA AAATGTAACT TCAAATAAAA GAAGGGGAAG AGCACCAAGT 
CAAAAAACCA ACAATCCTGA AACTAGCAAA ACCACTACTG AACCTGTTAA TAAAAATCAA 
GCAACTGAAG CTGTAGCCAA AAATACAGAT AATGCAGAAA GTCCTCCTAA ATCAAGAAGA 
GGCTTAAAAA GAAAGGCAGC CACCACTGAT GTTCCGGAAG GCAACACATC AGTAAGTGAA 
AATGGACCCC GTGCCAATGT AAGAGAAAGC ACAAGAGGAG CTTCAAGGCA AAAAACTGCA 
GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA TCACCAGCAA AGAAAAAGAG AACAGAGCCA 
TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT 
GAGAAACCAA CAGAAGTTGT AAAGACAAGG CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095812.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951301.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.

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CloneID	OXa45731
Clone ID Related Accession (Same CDS sequence)	XM_018095812.1
Accession Version	XM_018095812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951301.1
CDS	220..6918
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095812.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095812.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCAGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCA ACTACCCCTG CCAAGAGGTC ACCGGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTAA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095813.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951302.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.

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CloneID	OXa45732
Clone ID Related Accession (Same CDS sequence)	XM_018095813.1
Accession Version	XM_018095813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951302.1
CDS	220..6900
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095813.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095813.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCAAAG GCGGCATCCC CAGCCAAGAG GTCGCCGGCC 
AAGGCAGCTA CCCCGGCCAA GAGGTCGCCG GCAAAGGGGG CATCCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095814.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951303.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.

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CloneID	OXa45733
Clone ID Related Accession (Same CDS sequence)	XM_018095814.1
Accession Version	XM_018095814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCAAAGGCG 
GCATCCCCAG CCAAGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCA 
AAGGGGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951303.1
CDS	220..6900
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095814.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095814.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCG 
GCTAAGGCAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCAAAGGCG 
GCATCCCCAG CCAAGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCA 
AAGGGGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095815.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951304.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.

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CloneID	OXa45734
Clone ID Related Accession (Same CDS sequence)	XM_018095815.1
Accession Version	XM_018095815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCGGCT AAGGCAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGCAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGTAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCAAAGGCG 
GCATCCCCAG CCAAGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCA 
AAGGGGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951304.1
CDS	220..6900
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095815.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095815.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCGGCT AAGGCAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGCAGCTAC CCCGGCCAAG AGGTCGCCAG CCAAGGTAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT ACCCCGGCCA AGAGGTCGCC AGCAAAGGCG 
GCATCCCCAG CCAAGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCA 
AAGGGGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCAACT 
ACCCCTGCCA AGAGGTCACC GGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTAAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGTT CCCATAATTA TAGATAAGTC TGTTGAAAAG 
AATAGTACAC CAGAGCATGA AAAGAACCTG GAAGAAAGCA ATGAAGGAAC ATCTCTACAG 
GTACAGACCA TTGAAGATGC TTCTTCCACT AGTGCTGATA AAGCCACTCC GGCCAAAAGA 
AGAGGTCGTC CAGCTAAACG CAGCTTGAGT CATTCACCAG GTTTAGAAAA ACCTGTTGTA 
GATGTTCCAG AAGACCTAAG CACCAGCAAT GAGGTCTCTA ATATTCCTGA AGGATCAAGT 
CATATCAATA CAGACCAAGA GAACAATGTT GATTTACCAA TGGAAAAAGA GTCTGAACCG 
TTAAATCTAA ATATTGGAGC GAAAAAAGTC ACTAAGAAAT CTCAGAGAAA GAAAGTCACA 
GAAGAGTCTG TTCAAATAGC AGTGGAAAAC TTGGTTGTTG AACAAGATGA TGAAAAATCT 
GCTCCCCAAA GACGACGAGG AAGACCTCAA AGGGGTGGTC TTTCATCTAC CACTGAAGTT 
TTAGACGAAG CATTACCAGA AGAGCCTCCA ACAAACAGAC GAACTGGCAG GGGCCGAAAG 
ATTGCAGAAG TGGCAAAACC GCAACAAGTC AGTACAAGTG TTGAAGAATC TCAAGAAGTA 
AGAAACGAGG AACTCTCAGC AACTAAGACT CAGACTAAGT CTACCAGGCA TCAAAAAGCT 
GTATCTGAAG ACTCTACAGT TACAGCAACT AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG 
GACAGAGAAC AGAAAGCTGC TGAGGAGGAA AATACTGTGG AGAAATCACT TACAGAACTG 
CCAGGAGATT CAGCTTTAGT CAACAATGTA ACGCCATCTG TGAGAAGGGG TAGGCGAGCA 
AGAGGCAATG CAAATGCTGT GACTGAAACA GTCGTAACTG AAGCAAATTT GCCTGACTTG 
ACAAACAATA TCGTTTTACA TAAAGAGCAA GAAAGCTCCA GTCAAGCATC TCCAGAAGCC 
AAAACTCAAA GAGGAAAAAG GAAAAATCTT AAAAATACAA ACGTTGATTC TGTAAGCAAC 
TCTGCTGAAA CAGATGTTGC AACATTAGAA TTAGAAGAGA GACCTAACAA AAGATCTACA 
CGTGGTGTTA GAGGCACACA GCAAGTTGAT GAGCTTAAAG AACCAAAAGG AAAAAAAGCA 
AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA CTAGTTCATA TACAGGAGAA TCTAGCTAAA 
GAGAATGCAT CTAGGGCGAA ATCTGTGCAG TGGCACCCTC TTCTGACTGT TAAGGGGACA 
CCTGAAAAGA ATACTGAAGA GACTGTTAAG AATCTTGCAG ATGAAGAAGC TCCGGTCAGT 
GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA GAAGTAAAAG AATCTGTTCC AGCAAAACGA 
TCGCGTTCAA GAAGGGAGGA AAATAATGCA GAGGCATTAA CTTCTAGTAG TACAACAGCT 
GATGCAGTTC CTATTGCTTT GGTTGCCAGT GAAAGTACTG AAACTCCTAA GAATAAGAGA 
GGACGGAGGA AAGCAAACAC ACAATTAGAG AAAGTTACTG ATTCAGATCT TTCAGTGACA 
GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA GAGGTTGTAA ACGAAAAGGC CGTAAACAAA 
CGAAACACAG GTAAAAGAAA CCTCAAGTCT TCATCTGATA GTGATTCTAG TAGTAAAGCT 
GTTGAACCAG TGCTGGAGAC TATTACCCAA AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA 
AGTCTAGATT CTGAGACTAC TGAAGTTAAA ACTGTTGAGT CTGCTGAAAA TGTAACTTCA 
AATAAAAGAA GGGGAAGAGC ACCAAGTCAA AAAACCAACA ATCCTGAAAC TAGCAAAACC 
ACTACTGAAC CTGTTAATAA AAATCAAGCA ACTGAAGCTG TAGCCAAAAA TACAGATAAT 
GCAGAAAGTC CTCCTAAATC AAGAAGAGGC TTAAAAAGAA AGGCAGCCAC CACTGATGTT 
CCGGAAGGCA ACACATCAGT AAGTGAAAAT GGACCCCGTG CCAATGTAAG AGAAAGCACA 
AGAGGAGCTT CAAGGCAAAA AACTGCAGCA AAAGAAAATC CAGTGGTTGT TGAAGAATCA 
CCAGCAAAGA AAAAGAGAAC AGAGCCATCT TCCAGTCCTG CTCAGAAAGG AGGAAAAACA 
AGAGCCACTG CAGCAAAACG GAAAAATGAG AAACCAACAG AAGTTGTAAA GACAAGGCAA 
ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095816.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951305.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.

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CloneID	OXa45735
Clone ID Related Accession (Same CDS sequence)	XM_018095816.1
Accession Version	XM_018095816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCGG CTAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGCAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCCAAG GTAGCTACCC CGGCCAAGAG GTCGCCAGCC 
AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA GCCAAGGTAG CTACCCCGGC CAAGAGGTCG 
CCAGCAAAGG CGGCATCCCC AGCCAAGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CAAAGGGGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC AGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCAGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCAA CTACCCCTGC CAAGAGGTCA CCGGCCAAGG CAGCTACCCC TGCCAAGAGG 
TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG 
AGGTCTCCTG CTTCTACTTA TACAAAAGGA CGTTTCTCAA TTTCACGAAT TGATACACCA 
CCGCAAATAG ATGTCCATGC TCCTAACACT GAGAAAAATG AACTCTCTGC ACAAACACCC 
AGAAAATCAC GGAAGTCAAT ATCATTAAAG AAAACGCCAG GAAGAAGAAG TAGAAAACTT 
GAATCTTTTG AAATTTTGCG TTCCAGGCGA AGAAGTGGGG CAACTGAAGC TAACTTGCTT 
GTTGCAAAAT CATGGGCTGA TGTTGTAAGA AGCGGTGTTC CAAAAAATCA GAAGAAATCT 
GAAAAGCATG CTCGCAAAGC AGTACCTACC AAGAGAAAGC CAAAGCCAAA GACGCCAGCA 
CGAAGAATTA TTGACCGAAT TGGCACAGGA CATGCTGATT CTCCTGCCAC AATCCTTGTT 
GGAAGAGCTC ATACAAGAAC TGTCGACCTA ACTGGATATG TTCCTAAAAT TGTTCAAAAC 
CAAGCAATAA AAATCACCAA TGACCAAAAT GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT 
AATACCCCTG CACATGTAAA ACCAAGGAGA AGTGGTCGCT CAGGAGGCCT TCCGATGGAA 
ACTCCGCAAT CTATAATTGT TGATATGTCA CTGATGAAGA CTCCAGAGGA ATTAGGTGAA 
ATGGTGGTTT CTCCAATTAA TGGTAGCCCT GCTACAACAC AGAAGAAACA GTACAGTCGT 
GACGCAGTAT CTCGTCTTCT AAGTCCTGTG ACGCCAGCAT CAGAAGAACA GATATCCGAA 
GCTTCCAAAG ACACTCAGTT GGTTGTAAAA GAGACAGCCA CATTAAATGA AACCCGTTTT 
ATAAAAAAGG GTGCAAAGGC ACAGATGAAA AAAAGAGGAA CAATGGGTTT GACTGGTGTA 
AAAAGGATTA TGAGAACACC AAAACAAAAA GGAAAGCAGG TTATTGATCC TGTGGCGCTA 
AAGAAAATGT TGAGAACACC AAAACAAGTT GAATCACTTC CTGTAGAGTC CTCTGGCCCT 
AATGATCTAG TTGGACTGAA GGAAATCATG AAAACTCCAA AAGTAAAAGG GCAACCAGTG 
GAGGATATGA CTGGCATAGC ACGCATCATG AAGACCCCAA GACGCAAGCA ACAACCAGTA 
GAAGATATGG CTGGTATTAA ACGTATTATG AAGACTCCTA AACAGAAAGG GACGCCTGTT 
GAGGATATGA CCGGAATCAA GCGTATTATG ACGACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGTATTATG ACAACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGCATTATG AGAACCCCCA AGGAGAAAGG GCAACCTGTG 
GAGGATATGG AAGGTTTAAG TCAGATTATG AGAACACCAA CTGAAAATAC TCACCCAATA 
GAAGAAATAT TTGGGATGAA ACAACTAATA AAGACTCCTC CAAAGAGAGC ACTTGGTGTT 
AGAGAGGGCA CGTCTGGGAC ACCATCACAA TCTGGCAAAA AGGCTACTCT GTTTCAGACT 
AATCAAAAAT CGGCACAAGA AGAAGCATTG CCAAAACTTA CTTTGGAGAA AAATAATGAA 
AGCGAGGTTG CTGTACAAAC TGACAGAGAA ACTGAAAAAT CTAAACCAAC CCCCAAAAGA 
GGTCGACCTG CCAAGGCTAG TGTGAAGCTT GCTTCAGATG GTGGGAAGCC TGTTGATAAT 
GGCACATCTA CACATGAAAC ACACCCAGAA GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA 
CTGGCTAGTG ATGATAAATC CTCCACAGCT GCTGATAAAG CCACCCCAGC CAAACGAAGA 
GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT TCACCAGGTG GTGGAAATTC TGTTGAAAAC 
AACACATTCA GACATGAAAA AAATCCGGAA GAAGCCGCTC CTCTACAGGT ACTCTCCACT 
GAAAATACTT CATCCTTGGG TGATGATAAA GCTACTCCAG CTAAAAGAGG TCGTCCATGC 
AAACGTGGTA TGAGTAAGTC ACCAGCATCT AGTAATAAAG TTCCCATAAT TATAGATAAG 
TCTGTTGAAA AGAATAGTAC ACCAGAGCAT GAAAAGAACC TGGAAGAAAG CAATGAAGGA 
ACATCTCTAC AGGTACAGAC CATTGAAGAT GCTTCTTCCA CTAGTGCTGA TAAAGCCACT 
CCGGCCAAAA GAAGAGGTCG TCCAGCTAAA CGCAGCTTGA GTCATTCACC AGGTTTAGAA 
AAACCTGTTG TAGATGTTCC AGAAGACCTA AGCACCAGCA ATGAGGTCTC TAATATTCCT 
GAAGGATCAA GTCATATCAA TACAGACCAA GAGAACAATG TTGATTTACC AATGGAAAAA 
GAGTCTGAAC CGTTAAATCT AAATATTGGA GCGAAAAAAG TCACTAAGAA ATCTCAGAGA 
AAGAAAGTCA CAGAAGAGTC TGTTCAAATA GCAGTGGAAA ACTTGGTTGT TGAACAAGAT 
GATGAAAAAT CTGCTCCCCA AAGACGACGA GGAAGACCTC AAAGGGGTGG TCTTTCATCT 
ACCACTGAAG TTTTAGACGA AGCATTACCA GAAGAGCCTC CAACAAACAG ACGAACTGGC 
AGGGGCCGAA AGATTGCAGA AGTGGCAAAA CCGCAACAAG TCAGTACAAG TGTTGAAGAA 
TCTCAAGAAG TAAGAAACGA GGAACTCTCA GCAACTAAGA CTCAGACTAA GTCTACCAGG 
CATCAAAAAG CTGTATCTGA AGACTCTACA GTTACAGCAA CTAAGAAGCC ACGTGGAAGG 
CAACCTAAAG TGGACAGAGA ACAGAAAGCT GCTGAGGAGG AAAATACTGT GGAGAAATCA 
CTTACAGAAC TGCCAGGAGA TTCAGCTTTA GTCAACAATG TAACGCCATC TGTGAGAAGG 
GGTAGGCGAG CAAGAGGCAA TGCAAATGCT GTGACTGAAA CAGTCGTAAC TGAAGCAAAT 
TTGCCTGACT TGACAAACAA TATCGTTTTA CATAAAGAGC AAGAAAGCTC CAGTCAAGCA 
TCTCCAGAAG CCAAAACTCA AAGAGGAAAA AGGAAAAATC TTAAAAATAC AAACGTTGAT 
TCTGTAAGCA ACTCTGCTGA AACAGATGTT GCAACATTAG AATTAGAAGA GAGACCTAAC 
AAAAGATCTA CACGTGGTGT TAGAGGCACA CAGCAAGTTG ATGAGCTTAA AGAACCAAAA 
GGAAAAAAAG CAAAAGTAGA ACATGTGCCC AAACCTGCTG AACTAGTTCA TATACAGGAG 
AATCTAGCTA AAGAGAATGC ATCTAGGGCG AAATCTGTGC AGTGGCACCC TCTTCTGACT 
GTTAAGGGGA CACCTGAAAA GAATACTGAA GAGACTGTTA AGAATCTTGC AGATGAAGAA 
GCTCCGGTCA GTGCAGTAAA ATCTAGAGGA AGGAGGCGGC AAGAAGTAAA AGAATCTGTT 
CCAGCAAAAC GATCGCGTTC AAGAAGGGAG GAAAATAATG CAGAGGCATT AACTTCTAGT 
AGTACAACAG CTGATGCAGT TCCTATTGCT TTGGTTGCCA GTGAAAGTAC TGAAACTCCT 
AAGAATAAGA GAGGACGGAG GAAAGCAAAC ACACAATTAG AGAAAGTTAC TGATTCAGAT 
CTTTCAGTGA CAGAGGTTTC CTCTAAGCTA GACCTAGTGC CAGAGGTTGT AAACGAAAAG 
GCCGTAAACA AACGAAACAC AGGTAAAAGA AACCTCAAGT CTTCATCTGA TAGTGATTCT 
AGTAGTAAAG CTGTTGAACC AGTGCTGGAG ACTATTACCC AAAAGCGTGG ACGAAGAGGA 
GCAGCAGCCA AAAGTCTAGA TTCTGAGACT ACTGAAGTTA AAACTGTTGA GTCTGCTGAA 
AATGTAACTT CAAATAAAAG AAGGGGAAGA GCACCAAGTC AAAAAACCAA CAATCCTGAA 
ACTAGCAAAA CCACTACTGA ACCTGTTAAT AAAAATCAAG CAACTGAAGC TGTAGCCAAA 
AATACAGATA ATGCAGAAAG TCCTCCTAAA TCAAGAAGAG GCTTAAAAAG AAAGGCAGCC 
ACCACTGATG TTCCGGAAGG CAACACATCA GTAAGTGAAA ATGGACCCCG TGCCAATGTA 
AGAGAAAGCA CAAGAGGAGC TTCAAGGCAA AAAACTGCAG CAAAAGAAAA TCCAGTGGTT 
GTTGAAGAAT CACCAGCAAA GAAAAAGAGA ACAGAGCCAT CTTCCAGTCC TGCTCAGAAA 
GGAGGAAAAA CAAGAGCCAC TGCAGCAAAA CGGAAAAATG AGAAACCAAC AGAAGTTGTA 
AAGACAAGGC AAACATTGCG CACACGGAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951305.1
CDS	220..6852
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095816.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095816.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCGG CTAAGGCAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC CAAGGCAGCT ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA 
GCTACCCCGG CCAAGAGGTC GCCAGCCAAG GTAGCTACCC CGGCCAAGAG GTCGCCAGCC 
AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA GCCAAGGTAG CTACCCCGGC CAAGAGGTCG 
CCAGCAAAGG CGGCATCCCC AGCCAAGAGG TCGCCGGCCA AGGCAGCTAC CCCGGCCAAG 
AGGTCGCCGG CAAAGGGGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC AGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCAGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCAA CTACCCCTGC CAAGAGGTCA CCGGCCAAGG CAGCTACCCC TGCCAAGAGG 
TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG 
AGGTCTCCTG CTTCTACTTA TACAAAAGGA CGTTTCTCAA TTTCACGAAT TGATACACCA 
CCGCAAATAG ATGTCCATGC TCCTAACACT GAGAAAAATG AACTCTCTGC ACAAACACCC 
AGAAAATCAC GGAAGTCAAT ATCATTAAAG AAAACGCCAG GAAGAAGAAG TAGAAAACTT 
GAATCTTTTG AAATTTTGCG TTCCAGGCGA AGAAGTGGGG CAACTGAAGC TAACTTGCTT 
GTTGCAAAAT CATGGGCTGA TGTTGTAAGA AGCGGTGTTC CAAAAAATCA GAAGAAATCT 
GAAAAGCATG CTCGCAAAGC AGTACCTACC AAGAGAAAGC CAAAGCCAAA GACGCCAGCA 
CGAAGAATTA TTGACCGAAT TGGCACAGGA CATGCTGATT CTCCTGCCAC AATCCTTGTT 
GGAAGAGCTC ATACAAGAAC TGTCGACCTA ACTGGATATG TTCCTAAAAT TGTTCAAAAC 
CAAGCAATAA AAATCACCAA TGACCAAAAT GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT 
AATACCCCTG CACATGTAAA ACCAAGGAGA AGTGGTCGCT CAGGAGGCCT TCCGATGGAA 
ACTCCGCAAT CTATAATTGT TGATATGTCA CTGATGAAGA CTCCAGAGGA ATTAGGTGAA 
ATGGTGGTTT CTCCAATTAA TGGTAGCCCT GCTACAACAC AGAAGAAACA GTACAGTCGT 
GACGCAGTAT CTCGTCTTCT AAGTCCTGTG ACGCCAGCAT CAGAAGAACA GATATCCGAA 
GCTTCCAAAG ACACTCAGTT GGTTGTAAAA GAGACAGCCA CATTAAATGA AACCCGTTTT 
ATAAAAAAGG GTGCAAAGGC ACAGATGAAA AAAAGAGGAA CAATGGGTTT GACTGGTGTA 
AAAAGGATTA TGAGAACACC AAAACAAAAA GGAAAGCAGG TTATTGATCC TGTGGCGCTA 
AAGAAAATGT TGAGAACACC AAAACAAGTT GAATCACTTC CTGTAGAGTC CTCTGGCCCT 
AATGATCTAG TTGGACTGAA GGAAATCATG AAAACTCCAA AAGTAAAAGG GCAACCAGTG 
GAGGATATGA CTGGCATAGC ACGCATCATG AAGACCCCAA GACGCAAGCA ACAACCAGTA 
GAAGATATGG CTGGTATTAA ACGTATTATG AAGACTCCTA AACAGAAAGG GACGCCTGTT 
GAGGATATGA CCGGAATCAA GCGTATTATG ACGACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGTATTATG ACAACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGCATTATG AGAACCCCCA AGGAGAAAGG GCAACCTGTG 
GAGGATATGG AAGGTTTAAG TCAGATTATG AGAACACCAA CTGAAAATAC TCACCCAATA 
GAAGAAATAT TTGGGATGAA ACAACTAATA AAGACTCCTC CAAAGAGAGC ACTTGGTGTT 
AGAGAGGGCA CGTCTGGGAC ACCATCACAA TCTGGCAAAA AGGCTACTCT GTTTCAGACT 
AATCAAAAAT CGGCACAAGA AGAAGCATTG CCAAAACTTA CTTTGGAGAA AAATAATGAA 
AGCGAGGTTG CTGTACAAAC TGACAGAGAA ACTGAAAAAT CTAAACCAAC CCCCAAAAGA 
GGTCGACCTG CCAAGGCTAG TGTGAAGCTT GCTTCAGATG GTGGGAAGCC TGTTGATAAT 
GGCACATCTA CACATGAAAC ACACCCAGAA GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA 
CTGGCTAGTG ATGATAAATC CTCCACAGCT GCTGATAAAG CCACCCCAGC CAAACGAAGA 
GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT TCACCAGGTG GTGGAAATTC TGTTGAAAAC 
AACACATTCA GACATGAAAA AAATCCGGAA GAAGCCGCTC CTCTACAGGT ACTCTCCACT 
GAAAATACTT CATCCTTGGG TGATGATAAA GCTACTCCAG CTAAAAGAGG TCGTCCATGC 
AAACGTGGTA TGAGTAAGTC ACCAGCATCT AGTAATAAAG TTCCCATAAT TATAGATAAG 
TCTGTTGAAA AGAATAGTAC ACCAGAGCAT GAAAAGAACC TGGAAGAAAG CAATGAAGGA 
ACATCTCTAC AGGTACAGAC CATTGAAGAT GCTTCTTCCA CTAGTGCTGA TAAAGCCACT 
CCGGCCAAAA GAAGAGGTCG TCCAGCTAAA CGCAGCTTGA GTCATTCACC AGGTTTAGAA 
AAACCTGTTG TAGATGTTCC AGAAGACCTA AGCACCAGCA ATGAGGTCTC TAATATTCCT 
GAAGGATCAA GTCATATCAA TACAGACCAA GAGAACAATG TTGATTTACC AATGGAAAAA 
GAGTCTGAAC CGTTAAATCT AAATATTGGA GCGAAAAAAG TCACTAAGAA ATCTCAGAGA 
AAGAAAGTCA CAGAAGAGTC TGTTCAAATA GCAGTGGAAA ACTTGGTTGT TGAACAAGAT 
GATGAAAAAT CTGCTCCCCA AAGACGACGA GGAAGACCTC AAAGGGGTGG TCTTTCATCT 
ACCACTGAAG TTTTAGACGA AGCATTACCA GAAGAGCCTC CAACAAACAG ACGAACTGGC 
AGGGGCCGAA AGATTGCAGA AGTGGCAAAA CCGCAACAAG TCAGTACAAG TGTTGAAGAA 
TCTCAAGAAG TAAGAAACGA GGAACTCTCA GCAACTAAGA CTCAGACTAA GTCTACCAGG 
CATCAAAAAG CTGTATCTGA AGACTCTACA GTTACAGCAA CTAAGAAGCC ACGTGGAAGG 
CAACCTAAAG TGGACAGAGA ACAGAAAGCT GCTGAGGAGG AAAATACTGT GGAGAAATCA 
CTTACAGAAC TGCCAGGAGA TTCAGCTTTA GTCAACAATG TAACGCCATC TGTGAGAAGG 
GGTAGGCGAG CAAGAGGCAA TGCAAATGCT GTGACTGAAA CAGTCGTAAC TGAAGCAAAT 
TTGCCTGACT TGACAAACAA TATCGTTTTA CATAAAGAGC AAGAAAGCTC CAGTCAAGCA 
TCTCCAGAAG CCAAAACTCA AAGAGGAAAA AGGAAAAATC TTAAAAATAC AAACGTTGAT 
TCTGTAAGCA ACTCTGCTGA AACAGATGTT GCAACATTAG AATTAGAAGA GAGACCTAAC 
AAAAGATCTA CACGTGGTGT TAGAGGCACA CAGCAAGTTG ATGAGCTTAA AGAACCAAAA 
GGAAAAAAAG CAAAAGTAGA ACATGTGCCC AAACCTGCTG AACTAGTTCA TATACAGGAG 
AATCTAGCTA AAGAGAATGC ATCTAGGGCG AAATCTGTGC AGTGGCACCC TCTTCTGACT 
GTTAAGGGGA CACCTGAAAA GAATACTGAA GAGACTGTTA AGAATCTTGC AGATGAAGAA 
GCTCCGGTCA GTGCAGTAAA ATCTAGAGGA AGGAGGCGGC AAGAAGTAAA AGAATCTGTT 
CCAGCAAAAC GATCGCGTTC AAGAAGGGAG GAAAATAATG CAGAGGCATT AACTTCTAGT 
AGTACAACAG CTGATGCAGT TCCTATTGCT TTGGTTGCCA GTGAAAGTAC TGAAACTCCT 
AAGAATAAGA GAGGACGGAG GAAAGCAAAC ACACAATTAG AGAAAGTTAC TGATTCAGAT 
CTTTCAGTGA CAGAGGTTTC CTCTAAGCTA GACCTAGTGC CAGAGGTTGT AAACGAAAAG 
GCCGTAAACA AACGAAACAC AGGTAAAAGA AACCTCAAGT CTTCATCTGA TAGTGATTCT 
AGTAGTAAAG CTGTTGAACC AGTGCTGGAG ACTATTACCC AAAAGCGTGG ACGAAGAGGA 
GCAGCAGCCA AAAGTCTAGA TTCTGAGACT ACTGAAGTTA AAACTGTTGA GTCTGCTGAA 
AATGTAACTT CAAATAAAAG AAGGGGAAGA GCACCAAGTC AAAAAACCAA CAATCCTGAA 
ACTAGCAAAA CCACTACTGA ACCTGTTAAT AAAAATCAAG CAACTGAAGC TGTAGCCAAA 
AATACAGATA ATGCAGAAAG TCCTCCTAAA TCAAGAAGAG GCTTAAAAAG AAAGGCAGCC 
ACCACTGATG TTCCGGAAGG CAACACATCA GTAAGTGAAA ATGGACCCCG TGCCAATGTA 
AGAGAAAGCA CAAGAGGAGC TTCAAGGCAA AAAACTGCAG CAAAAGAAAA TCCAGTGGTT 
GTTGAAGAAT CACCAGCAAA GAAAAAGAGA ACAGAGCCAT CTTCCAGTCC TGCTCAGAAA 
GGAGGAAAAA CAAGAGCCAC TGCAGCAAAA CGGAAAAATG AGAAACCAAC AGAAGTTGTA 
AAGACAAGGC AAACATTGCG CACACGGAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095817.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951306.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.

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CloneID	OXa45736
Clone ID Related Accession (Same CDS sequence)	XM_018095817.1
Accession Version	XM_018095817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCAG CCAAGGTAGC TACCCCGGCC AAGAGGTCGC CAGCCAAGGT AGCTACCCCG 
GCCAAGAGGT CGCCAGCCAA GGCAGCTACC CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT 
ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA GCTACCCCGG CCAAGAGGTC GCCAGCCAAG 
GTAGCTACCC CGGCCAAGAG GTCGCCAGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCAAAGGCGG CATCCCCAGC CAAGAGGTCG CCGGCCAAGG CAGCTACCCC GGCCAAGAGG 
TCGCCGGCAA AGGGGGCATC CCCTGCCAGG AGGTCGCCGG CCAAGGCAGC TACCCCTGCC 
AGGAGGTCGC CGGCCAAGGC AGCTACCCCT GCCAGGAGGT CGCCGGCCAA GGCAGCTACC 
CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCCAAG GCGGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCAACTA CCCCTGCCAA GAGGTCACCG GCCAAGGCAG CTACCCCTGC CAAGAGGTCG 
CCAGTCAAGG CAGCTACCCC TGCCAAGAGG TCTCCTGCCT CAGCAACCCC TGCCAAGAGG 
TCTCCTGCTT CTACTTATAC AAAAGGACGT TTCTCAATTT CACGAATTGA TACACCACCG 
CAAATAGATG TCCATGCTCC TAACACTGAG AAAAATGAAC TCTCTGCACA AACACCCAGA 
AAATCACGGA AGTCAATATC ATTAAAGAAA ACGCCAGGAA GAAGAAGTAG AAAACTTGAA 
TCTTTTGAAA TTTTGCGTTC CAGGCGAAGA AGTGGGGCAA CTGAAGCTAA CTTGCTTGTT 
GCAAAATCAT GGGCTGATGT TGTAAGAAGC GGTGTTCCAA AAAATCAGAA GAAATCTGAA 
AAGCATGCTC GCAAAGCAGT ACCTACCAAG AGAAAGCCAA AGCCAAAGAC GCCAGCACGA 
AGAATTATTG ACCGAATTGG CACAGGACAT GCTGATTCTC CTGCCACAAT CCTTGTTGGA 
AGAGCTCATA CAAGAACTGT CGACCTAACT GGATATGTTC CTAAAATTGT TCAAAACCAA 
GCAATAAAAA TCACCAATGA CCAAAATGAA AGCTTTACAG GTTTGAGTGA GTTGTTTAAT 
ACCCCTGCAC ATGTAAAACC AAGGAGAAGT GGTCGCTCAG GAGGCCTTCC GATGGAAACT 
CCGCAATCTA TAATTGTTGA TATGTCACTG ATGAAGACTC CAGAGGAATT AGGTGAAATG 
GTGGTTTCTC CAATTAATGG TAGCCCTGCT ACAACACAGA AGAAACAGTA CAGTCGTGAC 
GCAGTATCTC GTCTTCTAAG TCCTGTGACG CCAGCATCAG AAGAACAGAT ATCCGAAGCT 
TCCAAAGACA CTCAGTTGGT TGTAAAAGAG ACAGCCACAT TAAATGAAAC CCGTTTTATA 
AAAAAGGGTG CAAAGGCACA GATGAAAAAA AGAGGAACAA TGGGTTTGAC TGGTGTAAAA 
AGGATTATGA GAACACCAAA ACAAAAAGGA AAGCAGGTTA TTGATCCTGT GGCGCTAAAG 
AAAATGTTGA GAACACCAAA ACAAGTTGAA TCACTTCCTG TAGAGTCCTC TGGCCCTAAT 
GATCTAGTTG GACTGAAGGA AATCATGAAA ACTCCAAAAG TAAAAGGGCA ACCAGTGGAG 
GATATGACTG GCATAGCACG CATCATGAAG ACCCCAAGAC GCAAGCAACA ACCAGTAGAA 
GATATGGCTG GTATTAAACG TATTATGAAG ACTCCTAAAC AGAAAGGGAC GCCTGTTGAG 
GATATGACCG GAATCAAGCG TATTATGACG ACCCCCAAGG AGAAAGGGCA ACCCGTGGAG 
GACATGGTGG GAATCAAACG TATTATGACA ACCCCCAAGG AGAAAGGGCA ACCCGTGGAG 
GACATGGTGG GAATCAAACG CATTATGAGA ACCCCCAAGG AGAAAGGGCA ACCTGTGGAG 
GATATGGAAG GTTTAAGTCA GATTATGAGA ACACCAACTG AAAATACTCA CCCAATAGAA 
GAAATATTTG GGATGAAACA ACTAATAAAG ACTCCTCCAA AGAGAGCACT TGGTGTTAGA 
GAGGGCACGT CTGGGACACC ATCACAATCT GGCAAAAAGG CTACTCTGTT TCAGACTAAT 
CAAAAATCGG CACAAGAAGA AGCATTGCCA AAACTTACTT TGGAGAAAAA TAATGAAAGC 
GAGGTTGCTG TACAAACTGA CAGAGAAACT GAAAAATCTA AACCAACCCC CAAAAGAGGT 
CGACCTGCCA AGGCTAGTGT GAAGCTTGCT TCAGATGGTG GGAAGCCTGT TGATAATGGC 
ACATCTACAC ATGAAACACA CCCAGAAGAA AGCAGCAGAG AGGCTTCTCT CCAAGTACTG 
GCTAGTGATG ATAAATCCTC CACAGCTGCT GATAAAGCCA CCCCAGCCAA ACGAAGAGGT 
CGTCCATCCA AGTCCAGTTT GAGTGGTTCA CCAGGTGGTG GAAATTCTGT TGAAAACAAC 
ACATTCAGAC ATGAAAAAAA TCCGGAAGAA GCCGCTCCTC TACAGGTACT CTCCACTGAA 
AATACTTCAT CCTTGGGTGA TGATAAAGCT ACTCCAGCTA AAAGAGGTCG TCCATGCAAA 
CGTGGTATGA GTAAGTCACC AGCATCTAGT AATAAAGTTC CCATAATTAT AGATAAGTCT 
GTTGAAAAGA ATAGTACACC AGAGCATGAA AAGAACCTGG AAGAAAGCAA TGAAGGAACA 
TCTCTACAGG TACAGACCAT TGAAGATGCT TCTTCCACTA GTGCTGATAA AGCCACTCCG 
GCCAAAAGAA GAGGTCGTCC AGCTAAACGC AGCTTGAGTC ATTCACCAGG TTTAGAAAAA 
CCTGTTGTAG ATGTTCCAGA AGACCTAAGC ACCAGCAATG AGGTCTCTAA TATTCCTGAA 
GGATCAAGTC ATATCAATAC AGACCAAGAG AACAATGTTG ATTTACCAAT GGAAAAAGAG 
TCTGAACCGT TAAATCTAAA TATTGGAGCG AAAAAAGTCA CTAAGAAATC TCAGAGAAAG 
AAAGTCACAG AAGAGTCTGT TCAAATAGCA GTGGAAAACT TGGTTGTTGA ACAAGATGAT 
GAAAAATCTG CTCCCCAAAG ACGACGAGGA AGACCTCAAA GGGGTGGTCT TTCATCTACC 
ACTGAAGTTT TAGACGAAGC ATTACCAGAA GAGCCTCCAA CAAACAGACG AACTGGCAGG 
GGCCGAAAGA TTGCAGAAGT GGCAAAACCG CAACAAGTCA GTACAAGTGT TGAAGAATCT 
CAAGAAGTAA GAAACGAGGA ACTCTCAGCA ACTAAGACTC AGACTAAGTC TACCAGGCAT 
CAAAAAGCTG TATCTGAAGA CTCTACAGTT ACAGCAACTA AGAAGCCACG TGGAAGGCAA 
CCTAAAGTGG ACAGAGAACA GAAAGCTGCT GAGGAGGAAA ATACTGTGGA GAAATCACTT 
ACAGAACTGC CAGGAGATTC AGCTTTAGTC AACAATGTAA CGCCATCTGT GAGAAGGGGT 
AGGCGAGCAA GAGGCAATGC AAATGCTGTG ACTGAAACAG TCGTAACTGA AGCAAATTTG 
CCTGACTTGA CAAACAATAT CGTTTTACAT AAAGAGCAAG AAAGCTCCAG TCAAGCATCT 
CCAGAAGCCA AAACTCAAAG AGGAAAAAGG AAAAATCTTA AAAATACAAA CGTTGATTCT 
GTAAGCAACT CTGCTGAAAC AGATGTTGCA ACATTAGAAT TAGAAGAGAG ACCTAACAAA 
AGATCTACAC GTGGTGTTAG AGGCACACAG CAAGTTGATG AGCTTAAAGA ACCAAAAGGA 
AAAAAAGCAA AAGTAGAACA TGTGCCCAAA CCTGCTGAAC TAGTTCATAT ACAGGAGAAT 
CTAGCTAAAG AGAATGCATC TAGGGCGAAA TCTGTGCAGT GGCACCCTCT TCTGACTGTT 
AAGGGGACAC CTGAAAAGAA TACTGAAGAG ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT 
CCGGTCAGTG CAGTAAAATC TAGAGGAAGG AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA 
GCAAAACGAT CGCGTTCAAG AAGGGAGGAA AATAATGCAG AGGCATTAAC TTCTAGTAGT 
ACAACAGCTG ATGCAGTTCC TATTGCTTTG GTTGCCAGTG AAAGTACTGA AACTCCTAAG 
AATAAGAGAG GACGGAGGAA AGCAAACACA CAATTAGAGA AAGTTACTGA TTCAGATCTT 
TCAGTGACAG AGGTTTCCTC TAAGCTAGAC CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC 
GTAAACAAAC GAAACACAGG TAAAAGAAAC CTCAAGTCTT CATCTGATAG TGATTCTAGT 
AGTAAAGCTG TTGAACCAGT GCTGGAGACT ATTACCCAAA AGCGTGGACG AAGAGGAGCA 
GCAGCCAAAA GTCTAGATTC TGAGACTACT GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT 
GTAACTTCAA ATAAAAGAAG GGGAAGAGCA CCAAGTCAAA AAACCAACAA TCCTGAAACT 
AGCAAAACCA CTACTGAACC TGTTAATAAA AATCAAGCAA CTGAAGCTGT AGCCAAAAAT 
ACAGATAATG CAGAAAGTCC TCCTAAATCA AGAAGAGGCT TAAAAAGAAA GGCAGCCACC 
ACTGATGTTC CGGAAGGCAA CACATCAGTA AGTGAAAATG GACCCCGTGC CAATGTAAGA 
GAAAGCACAA GAGGAGCTTC AAGGCAAAAA ACTGCAGCAA AAGAAAATCC AGTGGTTGTT 
GAAGAATCAC CAGCAAAGAA AAAGAGAACA GAGCCATCTT CCAGTCCTGC TCAGAAAGGA 
GGAAAAACAA GAGCCACTGC AGCAAAACGG AAAAATGAGA AACCAACAGA AGTTGTAAAG 
ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951306.1
CDS	220..6669
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095817.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095817.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGAATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCAGC CAAGGGGGCA 
TCCCCAGCCA AGAAGTCGCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCAG CCAAGGTAGC TACCCCGGCC AAGAGGTCGC CAGCCAAGGT AGCTACCCCG 
GCCAAGAGGT CGCCAGCCAA GGCAGCTACC CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT 
ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA GCTACCCCGG CCAAGAGGTC GCCAGCCAAG 
GTAGCTACCC CGGCCAAGAG GTCGCCAGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCAAAGGCGG CATCCCCAGC CAAGAGGTCG CCGGCCAAGG CAGCTACCCC GGCCAAGAGG 
TCGCCGGCAA AGGGGGCATC CCCTGCCAGG AGGTCGCCGG CCAAGGCAGC TACCCCTGCC 
AGGAGGTCGC CGGCCAAGGC AGCTACCCCT GCCAGGAGGT CGCCGGCCAA GGCAGCTACC 
CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCCAAG GCGGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCAACTA CCCCTGCCAA GAGGTCACCG GCCAAGGCAG CTACCCCTGC CAAGAGGTCG 
CCAGTCAAGG CAGCTACCCC TGCCAAGAGG TCTCCTGCCT CAGCAACCCC TGCCAAGAGG 
TCTCCTGCTT CTACTTATAC AAAAGGACGT TTCTCAATTT CACGAATTGA TACACCACCG 
CAAATAGATG TCCATGCTCC TAACACTGAG AAAAATGAAC TCTCTGCACA AACACCCAGA 
AAATCACGGA AGTCAATATC ATTAAAGAAA ACGCCAGGAA GAAGAAGTAG AAAACTTGAA 
TCTTTTGAAA TTTTGCGTTC CAGGCGAAGA AGTGGGGCAA CTGAAGCTAA CTTGCTTGTT 
GCAAAATCAT GGGCTGATGT TGTAAGAAGC GGTGTTCCAA AAAATCAGAA GAAATCTGAA 
AAGCATGCTC GCAAAGCAGT ACCTACCAAG AGAAAGCCAA AGCCAAAGAC GCCAGCACGA 
AGAATTATTG ACCGAATTGG CACAGGACAT GCTGATTCTC CTGCCACAAT CCTTGTTGGA 
AGAGCTCATA CAAGAACTGT CGACCTAACT GGATATGTTC CTAAAATTGT TCAAAACCAA 
GCAATAAAAA TCACCAATGA CCAAAATGAA AGCTTTACAG GTTTGAGTGA GTTGTTTAAT 
ACCCCTGCAC ATGTAAAACC AAGGAGAAGT GGTCGCTCAG GAGGCCTTCC GATGGAAACT 
CCGCAATCTA TAATTGTTGA TATGTCACTG ATGAAGACTC CAGAGGAATT AGGTGAAATG 
GTGGTTTCTC CAATTAATGG TAGCCCTGCT ACAACACAGA AGAAACAGTA CAGTCGTGAC 
GCAGTATCTC GTCTTCTAAG TCCTGTGACG CCAGCATCAG AAGAACAGAT ATCCGAAGCT 
TCCAAAGACA CTCAGTTGGT TGTAAAAGAG ACAGCCACAT TAAATGAAAC CCGTTTTATA 
AAAAAGGGTG CAAAGGCACA GATGAAAAAA AGAGGAACAA TGGGTTTGAC TGGTGTAAAA 
AGGATTATGA GAACACCAAA ACAAAAAGGA AAGCAGGTTA TTGATCCTGT GGCGCTAAAG 
AAAATGTTGA GAACACCAAA ACAAGTTGAA TCACTTCCTG TAGAGTCCTC TGGCCCTAAT 
GATCTAGTTG GACTGAAGGA AATCATGAAA ACTCCAAAAG TAAAAGGGCA ACCAGTGGAG 
GATATGACTG GCATAGCACG CATCATGAAG ACCCCAAGAC GCAAGCAACA ACCAGTAGAA 
GATATGGCTG GTATTAAACG TATTATGAAG ACTCCTAAAC AGAAAGGGAC GCCTGTTGAG 
GATATGACCG GAATCAAGCG TATTATGACG ACCCCCAAGG AGAAAGGGCA ACCCGTGGAG 
GACATGGTGG GAATCAAACG TATTATGACA ACCCCCAAGG AGAAAGGGCA ACCCGTGGAG 
GACATGGTGG GAATCAAACG CATTATGAGA ACCCCCAAGG AGAAAGGGCA ACCTGTGGAG 
GATATGGAAG GTTTAAGTCA GATTATGAGA ACACCAACTG AAAATACTCA CCCAATAGAA 
GAAATATTTG GGATGAAACA ACTAATAAAG ACTCCTCCAA AGAGAGCACT TGGTGTTAGA 
GAGGGCACGT CTGGGACACC ATCACAATCT GGCAAAAAGG CTACTCTGTT TCAGACTAAT 
CAAAAATCGG CACAAGAAGA AGCATTGCCA AAACTTACTT TGGAGAAAAA TAATGAAAGC 
GAGGTTGCTG TACAAACTGA CAGAGAAACT GAAAAATCTA AACCAACCCC CAAAAGAGGT 
CGACCTGCCA AGGCTAGTGT GAAGCTTGCT TCAGATGGTG GGAAGCCTGT TGATAATGGC 
ACATCTACAC ATGAAACACA CCCAGAAGAA AGCAGCAGAG AGGCTTCTCT CCAAGTACTG 
GCTAGTGATG ATAAATCCTC CACAGCTGCT GATAAAGCCA CCCCAGCCAA ACGAAGAGGT 
CGTCCATCCA AGTCCAGTTT GAGTGGTTCA CCAGGTGGTG GAAATTCTGT TGAAAACAAC 
ACATTCAGAC ATGAAAAAAA TCCGGAAGAA GCCGCTCCTC TACAGGTACT CTCCACTGAA 
AATACTTCAT CCTTGGGTGA TGATAAAGCT ACTCCAGCTA AAAGAGGTCG TCCATGCAAA 
CGTGGTATGA GTAAGTCACC AGCATCTAGT AATAAAGTTC CCATAATTAT AGATAAGTCT 
GTTGAAAAGA ATAGTACACC AGAGCATGAA AAGAACCTGG AAGAAAGCAA TGAAGGAACA 
TCTCTACAGG TACAGACCAT TGAAGATGCT TCTTCCACTA GTGCTGATAA AGCCACTCCG 
GCCAAAAGAA GAGGTCGTCC AGCTAAACGC AGCTTGAGTC ATTCACCAGG TTTAGAAAAA 
CCTGTTGTAG ATGTTCCAGA AGACCTAAGC ACCAGCAATG AGGTCTCTAA TATTCCTGAA 
GGATCAAGTC ATATCAATAC AGACCAAGAG AACAATGTTG ATTTACCAAT GGAAAAAGAG 
TCTGAACCGT TAAATCTAAA TATTGGAGCG AAAAAAGTCA CTAAGAAATC TCAGAGAAAG 
AAAGTCACAG AAGAGTCTGT TCAAATAGCA GTGGAAAACT TGGTTGTTGA ACAAGATGAT 
GAAAAATCTG CTCCCCAAAG ACGACGAGGA AGACCTCAAA GGGGTGGTCT TTCATCTACC 
ACTGAAGTTT TAGACGAAGC ATTACCAGAA GAGCCTCCAA CAAACAGACG AACTGGCAGG 
GGCCGAAAGA TTGCAGAAGT GGCAAAACCG CAACAAGTCA GTACAAGTGT TGAAGAATCT 
CAAGAAGTAA GAAACGAGGA ACTCTCAGCA ACTAAGACTC AGACTAAGTC TACCAGGCAT 
CAAAAAGCTG TATCTGAAGA CTCTACAGTT ACAGCAACTA AGAAGCCACG TGGAAGGCAA 
CCTAAAGTGG ACAGAGAACA GAAAGCTGCT GAGGAGGAAA ATACTGTGGA GAAATCACTT 
ACAGAACTGC CAGGAGATTC AGCTTTAGTC AACAATGTAA CGCCATCTGT GAGAAGGGGT 
AGGCGAGCAA GAGGCAATGC AAATGCTGTG ACTGAAACAG TCGTAACTGA AGCAAATTTG 
CCTGACTTGA CAAACAATAT CGTTTTACAT AAAGAGCAAG AAAGCTCCAG TCAAGCATCT 
CCAGAAGCCA AAACTCAAAG AGGAAAAAGG AAAAATCTTA AAAATACAAA CGTTGATTCT 
GTAAGCAACT CTGCTGAAAC AGATGTTGCA ACATTAGAAT TAGAAGAGAG ACCTAACAAA 
AGATCTACAC GTGGTGTTAG AGGCACACAG CAAGTTGATG AGCTTAAAGA ACCAAAAGGA 
AAAAAAGCAA AAGTAGAACA TGTGCCCAAA CCTGCTGAAC TAGTTCATAT ACAGGAGAAT 
CTAGCTAAAG AGAATGCATC TAGGGCGAAA TCTGTGCAGT GGCACCCTCT TCTGACTGTT 
AAGGGGACAC CTGAAAAGAA TACTGAAGAG ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT 
CCGGTCAGTG CAGTAAAATC TAGAGGAAGG AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA 
GCAAAACGAT CGCGTTCAAG AAGGGAGGAA AATAATGCAG AGGCATTAAC TTCTAGTAGT 
ACAACAGCTG ATGCAGTTCC TATTGCTTTG GTTGCCAGTG AAAGTACTGA AACTCCTAAG 
AATAAGAGAG GACGGAGGAA AGCAAACACA CAATTAGAGA AAGTTACTGA TTCAGATCTT 
TCAGTGACAG AGGTTTCCTC TAAGCTAGAC CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC 
GTAAACAAAC GAAACACAGG TAAAAGAAAC CTCAAGTCTT CATCTGATAG TGATTCTAGT 
AGTAAAGCTG TTGAACCAGT GCTGGAGACT ATTACCCAAA AGCGTGGACG AAGAGGAGCA 
GCAGCCAAAA GTCTAGATTC TGAGACTACT GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT 
GTAACTTCAA ATAAAAGAAG GGGAAGAGCA CCAAGTCAAA AAACCAACAA TCCTGAAACT 
AGCAAAACCA CTACTGAACC TGTTAATAAA AATCAAGCAA CTGAAGCTGT AGCCAAAAAT 
ACAGATAATG CAGAAAGTCC TCCTAAATCA AGAAGAGGCT TAAAAAGAAA GGCAGCCACC 
ACTGATGTTC CGGAAGGCAA CACATCAGTA AGTGAAAATG GACCCCGTGC CAATGTAAGA 
GAAAGCACAA GAGGAGCTTC AAGGCAAAAA ACTGCAGCAA AAGAAAATCC AGTGGTTGTT 
GAAGAATCAC CAGCAAAGAA AAAGAGAACA GAGCCATCTT CCAGTCCTGC TCAGAAAGGA 
GGAAAAACAA GAGCCACTGC AGCAAAACGG AAAAATGAGA AACCAACAGA AGTTGTAAAG 
ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_018095818.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951307.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.

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CloneID	OXa45737
Clone ID Related Accession (Same CDS sequence)	XM_018095818.1
Accession Version	XM_018095818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	antigen KI-67 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAAAA GTAAAGAGTT AAATCTAGAG CATGCAAAAA CACCAGCAAA GCTAAACATG 
GAGAATGGAG ATCATATCAG TACTCCTGTC ACAACTAGAA GGAGCGTTGG AAATGCTAAA 
GAAACGCAAG AGTTGAGCAT TGGAGAATTG CAGTTTGAAG GTCAGGAACT TCATGTTGAA 
TCGGCAACCT TGTCAGAGAA AACTGCCAGT GCCTTAAAAA GTCCATCTAG AGGGCGTCAG 
TCCACTCTGT CAAAACAAGA GCAACAAAGG CTTAGTGGTA AAAGAGTCTC TCCTGCCAAA 
TCTGAGCTGC TTCTTAAAGA TACAGCTAGC TTTCAAGATA ATGGGCAAAA ACAGACCAAT 
GACAAGCTCA CTCCAAATCG GAGACGTAGT ACTTCCAATG AACCTGCTCA AGATGTGCAA 
ATGACACCTG GAAAATCTGG AGGTTCCGTT TCACCTCGGT CCAGAAACAT GTCTCCAAAA 
TCAGCATCTC CCAAAGGCTT GCACAAGAAC GTTAAAGGAT CAGCTAAAGT CTCAAACCCA 
CGACAAAAAC GACGTAGTTC TGAGCTTGAT TTGCCTGAGC CAACTCCAAA AAGAAAAAGG 
GTCTCATTTG GAGGACATCT AAGTCCAGAG CTTTTTGATA AAAGGCTTCC TCCCAACTCT 
CCTCTTAAAA GAGGTGCAAC ACCTGCTAGG CGGAGCTTAT CACTTGTCAC GCCTCTTGCA 
CTTATGCGAA AGAGCTTTGG TGGTTTCAGG CAAACCGCAA TTAAGGAAAC TTTTGAGCCT 
GGCACTGACT TAGCACTCTT CAAGGGATCT CCAGCCAAGA AGTCACCATC CAAGAGGTCG 
CCAGCCAAGG GGGCATCCCC AGCCAAGAAG TCGCCGGCCA AGAGGTCGCC CGCCAAGGGG 
GCATCCCCGG CCAAGAGGTC GCCCGCCAAG GGGGCATCCC CGGCCAAGAG GTCGCCCGCC 
AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC GCCAAGGGGG CATCCCCGGC CAAGAGGTCG 
CCCGCCAAGG GGGCATCCCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCGGCTAA GGCAGCTACC CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT 
ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA GCTACCCCGG CCAAGAGGTC GCCAGCCAAG 
GCAGCTACCC CGGCCAAGAG GTCGCCAGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CAAAGGCGGC ATCCCCAGCC 
AAGAGGTCGC CGGCCAAGGC AGCTACCCCG GCCAAGAGGT CGCCGGCAAA GGGGGCATCC 
CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT ACCCCTGCCA GGAGGTCGCC GGCCAAGGCA 
GCTACCCCTG CCAGGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG GCCAAGGCGG CTACCCCGGC CAAGAGGTCG 
CCGGCCAAGG CGGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCAACTAC CCCTGCCAAG 
AGGTCACCGG CCAAGGCAGC TACCCCTGCC AAGAGGTCGC CAGTCAAGGC AGCTACCCCT 
GCCAAGAGGT CTCCTGCCTC AGCAACCCCT GCCAAGAGGT CTCCTGCTTC TACTTATACA 
AAAGGACGTT TCTCAATTTC ACGAATTGAT ACACCACCGC AAATAGATGT CCATGCTCCT 
AACACTGAGA AAAATGAACT CTCTGCACAA ACACCCAGAA AATCACGGAA GTCAATATCA 
TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA AAACTTGAAT CTTTTGAAAT TTTGCGTTCC 
AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC TTGCTTGTTG CAAAATCATG GGCTGATGTT 
GTAAGAAGCG GTGTTCCAAA AAATCAGAAG AAATCTGAAA AGCATGCTCG CAAAGCAGTA 
CCTACCAAGA GAAAGCCAAA GCCAAAGACG CCAGCACGAA GAATTATTGA CCGAATTGGC 
ACAGGACATG CTGATTCTCC TGCCACAATC CTTGTTGGAA GAGCTCATAC AAGAACTGTC 
GACCTAACTG GATATGTTCC TAAAATTGTT CAAAACCAAG CAATAAAAAT CACCAATGAC 
CAAAATGAAA GCTTTACAGG TTTGAGTGAG TTGTTTAATA CCCCTGCACA TGTAAAACCA 
AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG ATGGAAACTC CGCAATCTAT AATTGTTGAT 
ATGTCACTGA TGAAGACTCC AGAGGAATTA GGTGAAATGG TGGTTTCTCC AATTAATGGT 
AGCCCTGCTA CAACACAGAA GAAACAGTAC AGTCGTGACG CAGTATCTCG TCTTCTAAGT 
CCTGTGACGC CAGCATCAGA AGAACAGATA TCCGAAGCTT CCAAAGACAC TCAGTTGGTT 
GTAAAAGAGA CAGCCACATT AAATGAAACC CGTTTTATAA AAAAGGGTGC AAAGGCACAG 
ATGAAAAAAA GAGGAACAAT GGGTTTGACT GGTGTAAAAA GGATTATGAG AACACCAAAA 
CAAAAAGGAA AGCAGGTTAT TGATCCTGTG GCGCTAAAGA AAATGTTGAG AACACCAAAA 
CAAGTTGAAT CACTTCCTGT AGAGTCCTCT GGCCCTAATG ATCTAGTTGG ACTGAAGGAA 
ATCATGAAAA CTCCAAAAGT AAAAGGGCAA CCAGTGGAGG ATATGACTGG CATAGCACGC 
ATCATGAAGA CCCCAAGACG CAAGCAACAA CCAGTAGAAG ATATGGCTGG TATTAAACGT 
ATTATGAAGA CTCCTAAACA GAAAGGGACG CCTGTTGAGG ATATGACCGG AATCAAGCGT 
ATTATGACGA CCCCCAAGGA GAAAGGGCAA CCCGTGGAGG ACATGGTGGG AATCAAACGT 
ATTATGACAA CCCCCAAGGA GAAAGGGCAA CCCGTGGAGG ACATGGTGGG AATCAAACGC 
ATTATGAGAA CCCCCAAGGA GAAAGGGCAA CCTGTGGAGG ATATGGAAGG TTTAAGTCAG 
ATTATGAGAA CACCAACTGA AAATACTCAC CCAATAGAAG AAATATTTGG GATGAAACAA 
CTAATAAAGA CTCCTCCAAA GAGAGCACTT GGTGTTAGAG AGGGCACGTC TGGGACACCA 
TCACAATCTG GCAAAAAGGC TACTCTGTTT CAGACTAATC AAAAATCGGC ACAAGAAGAA 
GCATTGCCAA AACTTACTTT GGAGAAAAAT AATGAAAGCG AGGTTGCTGT ACAAACTGAC 
AGAGAAACTG AAAAATCTAA ACCAACCCCC AAAAGAGGTC GACCTGCCAA GGCTAGTGTG 
AAGCTTGCTT CAGATGGTGG GAAGCCTGTT GATAATGGCA CATCTACACA TGAAACACAC 
CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC CAAGTACTGG CTAGTGATGA TAAATCCTCC 
ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA CGAAGAGGTC GTCCATCCAA GTCCAGTTTG 
AGTGGTTCAC CAGGTGGTGG AAATTCTGTT GAAAACAACA CATTCAGACA TGAAAAAAAT 
CCGGAAGAAG CCGCTCCTCT ACAGGTACTC TCCACTGAAA ATACTTCATC CTTGGGTGAT 
GATAAAGCTA CTCCAGCTAA AAGAGGTCGT CCATGCAAAC GTGGTATGAG TAAGTCACCA 
GCATCTAGTA ATAAAGTTCC CATAATTATA GATAAGTCTG TTGAAAAGAA TAGTACACCA 
GAGCATGAAA AGAACCTGGA AGAAAGCAAT GAAGGAACAT CTCTACAGGT ACAGACCATT 
GAAGATGCTT CTTCCACTAG TGCTGATAAA GCCACTCCGG CCAAAAGAAG AGGTCGTCCA 
GCTAAACGCA GCTTGAGTCA TTCACCAGGT TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA 
GACCTAAGCA CCAGCAATGA GGTCTCTAAT ATTCCTGAAG GATCAAGTCA TATCAATACA 
GACCAAGAGA ACAATGTTGA TTTACCAATG GAAAAAGAGT CTGAACCGTT AAATCTAAAT 
ATTGGAGCGA AAAAAGTCAC TAAGAAATCT CAGAGAAAGA AAGTCACAGA AGAGTCTGTT 
CAAATAGCAG TGGAAAACTT GGTTGTTGAA CAAGATGATG AAAAATCTGC TCCCCAAAGA 
CGACGAGGAA GACCTCAAAG GGGTGGTCTT TCATCTACCA CTGAAGTTTT AGACGAAGCA 
TTACCAGAAG AGCCTCCAAC AAACAGACGA ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG 
GCAAAACCGC AACAAGTCAG TACAAGTGTT GAAGAATCTC AAGAAGTAAG AAACGAGGAA 
CTCTCAGCAA CTAAGACTCA GACTAAGTCT ACCAGGCATC AAAAAGCTGT ATCTGAAGAC 
TCTACAGTTA CAGCAACTAA GAAGCCACGT GGAAGGCAAC CTAAAGTGGA CAGAGAACAG 
AAAGCTGCTG AGGAGGAAAA TACTGTGGAG AAATCACTTA CAGAACTGCC AGGAGATTCA 
GCTTTAGTCA ACAATGTAAC GCCATCTGTG AGAAGGGGTA GGCGAGCAAG AGGCAATGCA 
AATGCTGTGA CTGAAACAGT CGTAACTGAA GCAAATTTGC CTGACTTGAC AAACAATATC 
GTTTTACATA AAGAGCAAGA AAGCTCCAGT CAAGCATCTC CAGAAGCCAA AACTCAAAGA 
GGAAAAAGGA AAAATCTTAA AAATACAAAC GTTGATTCTG TAAGCAACTC TGCTGAAACA 
GATGTTGCAA CATTAGAATT AGAAGAGAGA CCTAACAAAA GATCTACACG TGGTGTTAGA 
GGCACACAGC AAGTTGATGA GCTTAAAGAA CCAAAAGGAA AAAAAGCAAA AGTAGAACAT 
GTGCCCAAAC CTGCTGAACT AGTTCATATA CAGGAGAATC TAGCTAAAGA GAATGCATCT 
AGGGCGAAAT CTGTGCAGTG GCACCCTCTT CTGACTGTTA AGGGGACACC TGAAAAGAAT 
ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT GAAGAAGCTC CGGTCAGTGC AGTAAAATCT 
AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA TCTGTTCCAG CAAAACGATC GCGTTCAAGA 
AGGGAGGAAA ATAATGCAGA GGCATTAACT TCTAGTAGTA CAACAGCTGA TGCAGTTCCT 
ATTGCTTTGG TTGCCAGTGA AAGTACTGAA ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA 
GCAAACACAC AATTAGAGAA AGTTACTGAT TCAGATCTTT CAGTGACAGA GGTTTCCTCT 
AAGCTAGACC TAGTGCCAGA GGTTGTAAAC GAAAAGGCCG TAAACAAACG AAACACAGGT 
AAAAGAAACC TCAAGTCTTC ATCTGATAGT GATTCTAGTA GTAAAGCTGT TGAACCAGTG 
CTGGAGACTA TTACCCAAAA GCGTGGACGA AGAGGAGCAG CAGCCAAAAG TCTAGATTCT 
GAGACTACTG AAGTTAAAAC TGTTGAGTCT GCTGAAAATG TAACTTCAAA TAAAAGAAGG 
GGAAGAGCAC CAAGTCAAAA AACCAACAAT CCTGAAACTA GCAAAACCAC TACTGAACCT 
GTTAATAAAA ATCAAGCAAC TGAAGCTGTA GCCAAAAATA CAGATAATGC AGAAAGTCCT 
CCTAAATCAA GAAGAGGCTT AAAAAGAAAG GCAGCCACCA CTGATGTTCC GGAAGGCAAC 
ACATCAGTAA GTGAAAATGG ACCCCGTGCC AATGTAAGAG AAAGCACAAG AGGAGCTTCA 
AGGCAAAAAA CTGCAGCAAA AGAAAATCCA GTGGTTGTTG AAGAATCACC AGCAAAGAAA 
AAGAGAACAG AGCCATCTTC CAGTCCTGCT CAGAAAGGAG GAAAAACAAG AGCCACTGCA 
GCAAAACGGA AAAATGAGAA ACCAACAGAA GTTGTAAAGA CAAGGCAAAC ATTGCGCACA 
CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951307.1
CDS	432..6620
Translation	MLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKKSPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKATTPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKVPIIIDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_018095818.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095818.1

ORF Insert Sequence:

ATGCTTAAAA GTAAAGAGTT AAATCTAGAG CATGCAAAAA CACCAGCAAA GCTAAACATG 
GAGAATGGAG ATCATATCAG TACTCCTGTC ACAACTAGAA GGAGCGTTGG AAATGCTAAA 
GAAACGCAAG AGTTGAGCAT TGGAGAATTG CAGTTTGAAG GTCAGGAACT TCATGTTGAA 
TCGGCAACCT TGTCAGAGAA AACTGCCAGT GCCTTAAAAA GTCCATCTAG AGGGCGTCAG 
TCCACTCTGT CAAAACAAGA GCAACAAAGG CTTAGTGGTA AAAGAGTCTC TCCTGCCAAA 
TCTGAGCTGC TTCTTAAAGA TACAGCTAGC TTTCAAGATA ATGGGCAAAA ACAGACCAAT 
GACAAGCTCA CTCCAAATCG GAGACGTAGT ACTTCCAATG AACCTGCTCA AGATGTGCAA 
ATGACACCTG GAAAATCTGG AGGTTCCGTT TCACCTCGGT CCAGAAACAT GTCTCCAAAA 
TCAGCATCTC CCAAAGGCTT GCACAAGAAC GTTAAAGGAT CAGCTAAAGT CTCAAACCCA 
CGACAAAAAC GACGTAGTTC TGAGCTTGAT TTGCCTGAGC CAACTCCAAA AAGAAAAAGG 
GTCTCATTTG GAGGACATCT AAGTCCAGAG CTTTTTGATA AAAGGCTTCC TCCCAACTCT 
CCTCTTAAAA GAGGTGCAAC ACCTGCTAGG CGGAGCTTAT CACTTGTCAC GCCTCTTGCA 
CTTATGCGAA AGAGCTTTGG TGGTTTCAGG CAAACCGCAA TTAAGGAAAC TTTTGAGCCT 
GGCACTGACT TAGCACTCTT CAAGGGATCT CCAGCCAAGA AGTCACCATC CAAGAGGTCG 
CCAGCCAAGG GGGCATCCCC AGCCAAGAAG TCGCCGGCCA AGAGGTCGCC CGCCAAGGGG 
GCATCCCCGG CCAAGAGGTC GCCCGCCAAG GGGGCATCCC CGGCCAAGAG GTCGCCCGCC 
AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC GCCAAGGGGG CATCCCCGGC CAAGAGGTCG 
CCCGCCAAGG GGGCATCCCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCGGCTAA GGCAGCTACC CCGGCCAAGA GGTCGCCAGC CAAGGTAGCT 
ACCCCGGCCA AGAGGTCGCC AGCCAAGGTA GCTACCCCGG CCAAGAGGTC GCCAGCCAAG 
GCAGCTACCC CGGCCAAGAG GTCGCCAGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCCAAGG TAGCTACCCC GGCCAAGAGG 
TCGCCAGCCA AGGTAGCTAC CCCGGCCAAG AGGTCGCCAG CAAAGGCGGC ATCCCCAGCC 
AAGAGGTCGC CGGCCAAGGC AGCTACCCCG GCCAAGAGGT CGCCGGCAAA GGGGGCATCC 
CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT ACCCCTGCCA GGAGGTCGCC GGCCAAGGCA 
GCTACCCCTG CCAGGAGGTC GCCGGCCAAG GCAGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG GCCAAGGCGG CTACCCCGGC CAAGAGGTCG 
CCGGCCAAGG CGGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCAACTAC CCCTGCCAAG 
AGGTCACCGG CCAAGGCAGC TACCCCTGCC AAGAGGTCGC CAGTCAAGGC AGCTACCCCT 
GCCAAGAGGT CTCCTGCCTC AGCAACCCCT GCCAAGAGGT CTCCTGCTTC TACTTATACA 
AAAGGACGTT TCTCAATTTC ACGAATTGAT ACACCACCGC AAATAGATGT CCATGCTCCT 
AACACTGAGA AAAATGAACT CTCTGCACAA ACACCCAGAA AATCACGGAA GTCAATATCA 
TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA AAACTTGAAT CTTTTGAAAT TTTGCGTTCC 
AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC TTGCTTGTTG CAAAATCATG GGCTGATGTT 
GTAAGAAGCG GTGTTCCAAA AAATCAGAAG AAATCTGAAA AGCATGCTCG CAAAGCAGTA 
CCTACCAAGA GAAAGCCAAA GCCAAAGACG CCAGCACGAA GAATTATTGA CCGAATTGGC 
ACAGGACATG CTGATTCTCC TGCCACAATC CTTGTTGGAA GAGCTCATAC AAGAACTGTC 
GACCTAACTG GATATGTTCC TAAAATTGTT CAAAACCAAG CAATAAAAAT CACCAATGAC 
CAAAATGAAA GCTTTACAGG TTTGAGTGAG TTGTTTAATA CCCCTGCACA TGTAAAACCA 
AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG ATGGAAACTC CGCAATCTAT AATTGTTGAT 
ATGTCACTGA TGAAGACTCC AGAGGAATTA GGTGAAATGG TGGTTTCTCC AATTAATGGT 
AGCCCTGCTA CAACACAGAA GAAACAGTAC AGTCGTGACG CAGTATCTCG TCTTCTAAGT 
CCTGTGACGC CAGCATCAGA AGAACAGATA TCCGAAGCTT CCAAAGACAC TCAGTTGGTT 
GTAAAAGAGA CAGCCACATT AAATGAAACC CGTTTTATAA AAAAGGGTGC AAAGGCACAG 
ATGAAAAAAA GAGGAACAAT GGGTTTGACT GGTGTAAAAA GGATTATGAG AACACCAAAA 
CAAAAAGGAA AGCAGGTTAT TGATCCTGTG GCGCTAAAGA AAATGTTGAG AACACCAAAA 
CAAGTTGAAT CACTTCCTGT AGAGTCCTCT GGCCCTAATG ATCTAGTTGG ACTGAAGGAA 
ATCATGAAAA CTCCAAAAGT AAAAGGGCAA CCAGTGGAGG ATATGACTGG CATAGCACGC 
ATCATGAAGA CCCCAAGACG CAAGCAACAA CCAGTAGAAG ATATGGCTGG TATTAAACGT 
ATTATGAAGA CTCCTAAACA GAAAGGGACG CCTGTTGAGG ATATGACCGG AATCAAGCGT 
ATTATGACGA CCCCCAAGGA GAAAGGGCAA CCCGTGGAGG ACATGGTGGG AATCAAACGT 
ATTATGACAA CCCCCAAGGA GAAAGGGCAA CCCGTGGAGG ACATGGTGGG AATCAAACGC 
ATTATGAGAA CCCCCAAGGA GAAAGGGCAA CCTGTGGAGG ATATGGAAGG TTTAAGTCAG 
ATTATGAGAA CACCAACTGA AAATACTCAC CCAATAGAAG AAATATTTGG GATGAAACAA 
CTAATAAAGA CTCCTCCAAA GAGAGCACTT GGTGTTAGAG AGGGCACGTC TGGGACACCA 
TCACAATCTG GCAAAAAGGC TACTCTGTTT CAGACTAATC AAAAATCGGC ACAAGAAGAA 
GCATTGCCAA AACTTACTTT GGAGAAAAAT AATGAAAGCG AGGTTGCTGT ACAAACTGAC 
AGAGAAACTG AAAAATCTAA ACCAACCCCC AAAAGAGGTC GACCTGCCAA GGCTAGTGTG 
AAGCTTGCTT CAGATGGTGG GAAGCCTGTT GATAATGGCA CATCTACACA TGAAACACAC 
CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC CAAGTACTGG CTAGTGATGA TAAATCCTCC 
ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA CGAAGAGGTC GTCCATCCAA GTCCAGTTTG 
AGTGGTTCAC CAGGTGGTGG AAATTCTGTT GAAAACAACA CATTCAGACA TGAAAAAAAT 
CCGGAAGAAG CCGCTCCTCT ACAGGTACTC TCCACTGAAA ATACTTCATC CTTGGGTGAT 
GATAAAGCTA CTCCAGCTAA AAGAGGTCGT CCATGCAAAC GTGGTATGAG TAAGTCACCA 
GCATCTAGTA ATAAAGTTCC CATAATTATA GATAAGTCTG TTGAAAAGAA TAGTACACCA 
GAGCATGAAA AGAACCTGGA AGAAAGCAAT GAAGGAACAT CTCTACAGGT ACAGACCATT 
GAAGATGCTT CTTCCACTAG TGCTGATAAA GCCACTCCGG CCAAAAGAAG AGGTCGTCCA 
GCTAAACGCA GCTTGAGTCA TTCACCAGGT TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA 
GACCTAAGCA CCAGCAATGA GGTCTCTAAT ATTCCTGAAG GATCAAGTCA TATCAATACA 
GACCAAGAGA ACAATGTTGA TTTACCAATG GAAAAAGAGT CTGAACCGTT AAATCTAAAT 
ATTGGAGCGA AAAAAGTCAC TAAGAAATCT CAGAGAAAGA AAGTCACAGA AGAGTCTGTT 
CAAATAGCAG TGGAAAACTT GGTTGTTGAA CAAGATGATG AAAAATCTGC TCCCCAAAGA 
CGACGAGGAA GACCTCAAAG GGGTGGTCTT TCATCTACCA CTGAAGTTTT AGACGAAGCA 
TTACCAGAAG AGCCTCCAAC AAACAGACGA ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG 
GCAAAACCGC AACAAGTCAG TACAAGTGTT GAAGAATCTC AAGAAGTAAG AAACGAGGAA 
CTCTCAGCAA CTAAGACTCA GACTAAGTCT ACCAGGCATC AAAAAGCTGT ATCTGAAGAC 
TCTACAGTTA CAGCAACTAA GAAGCCACGT GGAAGGCAAC CTAAAGTGGA CAGAGAACAG 
AAAGCTGCTG AGGAGGAAAA TACTGTGGAG AAATCACTTA CAGAACTGCC AGGAGATTCA 
GCTTTAGTCA ACAATGTAAC GCCATCTGTG AGAAGGGGTA GGCGAGCAAG AGGCAATGCA 
AATGCTGTGA CTGAAACAGT CGTAACTGAA GCAAATTTGC CTGACTTGAC AAACAATATC 
GTTTTACATA AAGAGCAAGA AAGCTCCAGT CAAGCATCTC CAGAAGCCAA AACTCAAAGA 
GGAAAAAGGA AAAATCTTAA AAATACAAAC GTTGATTCTG TAAGCAACTC TGCTGAAACA 
GATGTTGCAA CATTAGAATT AGAAGAGAGA CCTAACAAAA GATCTACACG TGGTGTTAGA 
GGCACACAGC AAGTTGATGA GCTTAAAGAA CCAAAAGGAA AAAAAGCAAA AGTAGAACAT 
GTGCCCAAAC CTGCTGAACT AGTTCATATA CAGGAGAATC TAGCTAAAGA GAATGCATCT 
AGGGCGAAAT CTGTGCAGTG GCACCCTCTT CTGACTGTTA AGGGGACACC TGAAAAGAAT 
ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT GAAGAAGCTC CGGTCAGTGC AGTAAAATCT 
AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA TCTGTTCCAG CAAAACGATC GCGTTCAAGA 
AGGGAGGAAA ATAATGCAGA GGCATTAACT TCTAGTAGTA CAACAGCTGA TGCAGTTCCT 
ATTGCTTTGG TTGCCAGTGA AAGTACTGAA ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA 
GCAAACACAC AATTAGAGAA AGTTACTGAT TCAGATCTTT CAGTGACAGA GGTTTCCTCT 
AAGCTAGACC TAGTGCCAGA GGTTGTAAAC GAAAAGGCCG TAAACAAACG AAACACAGGT 
AAAAGAAACC TCAAGTCTTC ATCTGATAGT GATTCTAGTA GTAAAGCTGT TGAACCAGTG 
CTGGAGACTA TTACCCAAAA GCGTGGACGA AGAGGAGCAG CAGCCAAAAG TCTAGATTCT 
GAGACTACTG AAGTTAAAAC TGTTGAGTCT GCTGAAAATG TAACTTCAAA TAAAAGAAGG 
GGAAGAGCAC CAAGTCAAAA AACCAACAAT CCTGAAACTA GCAAAACCAC TACTGAACCT 
GTTAATAAAA ATCAAGCAAC TGAAGCTGTA GCCAAAAATA CAGATAATGC AGAAAGTCCT 
CCTAAATCAA GAAGAGGCTT AAAAAGAAAG GCAGCCACCA CTGATGTTCC GGAAGGCAAC 
ACATCAGTAA GTGAAAATGG ACCCCGTGCC AATGTAAGAG AAAGCACAAG AGGAGCTTCA 
AGGCAAAAAA CTGCAGCAAA AGAAAATCCA GTGGTTGTTG AAGAATCACC AGCAAAGAAA 
AAGAGAACAG AGCCATCTTC CAGTCCTGCT CAGAAAGGAG GAAAAACAAG AGCCACTGCA 
GCAAAACGGA AAAATGAGAA ACCAACAGAA GTTGTAAAGA CAAGGCAAAC ATTGCGCACA 
CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905884.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761744.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.

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CloneID	OXa62322
Clone ID Related Accession (Same CDS sequence)	XM_031905884.1 , XM_031905887.1 , XM_031905886.1
Accession Version	XM_031905884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761744.1
CDS	214..6945
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905884.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905884.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905887.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761747.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.

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CloneID	OXa62322
Clone ID Related Accession (Same CDS sequence)	XM_031905884.1 , XM_031905887.1 , XM_031905886.1
Accession Version	XM_031905887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761747.1
CDS	135..6866
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905887.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905887.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905886.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761746.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.

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CloneID	OXa62322
Clone ID Related Accession (Same CDS sequence)	XM_031905884.1 , XM_031905887.1 , XM_031905886.1
Accession Version	XM_031905886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761746.1
CDS	122..6853
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905886.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905886.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCACCAGCC AAGGCAGCTA CCCCTGCCAA GAGGTCGCCA 
GTCAAGGCAG CTACCCCTGC CAAGAGGTCT CCTGCCTCAG CAACCCCTGC CAAGAGGTCT 
CCTGCTTCTA CTTATACAAA AGGACGTTTC TCAATTTCAC GAATTGATAC ACCACCGCAA 
ATAGATGTCC ATGCTCCTAA CACTGAGAAA AATGAACTCT CTGCACAAAC ACCCAGAAAA 
TCACGGAAGT CAATATCATT AAAGAAAACG CCAGGAAGAA GAAGTAGAAA ACTTGAATCT 
TTTGAAATTT TGCGTTCCAG GCGAAGAAGT GGGGCAACTG AAGCTAACTT GCTTGTTGCA 
AAATCATGGG CTGATGTTGT AAGAAGCGGT GTTCCAAAAA ATCAGAAGAA ATCTGAAAAG 
CATGCTCGCA AAGCAGTACC TACCAAGAGA AAGCCAAAGC CAAAGACGCC AGCACGAAGA 
ATTATTGACC GAATTGGCAC AGGACATGCT GATTCTCCTG CCACAATCCT TGTTGGAAGA 
GCTCATACAA GAACTGTCGA CCTAACTGGA TATGTTCCTA AAATTGTTCA AAACCAAGCA 
ATAAAAATCA CCAATGACCA AAATGAAAGC TTTACAGGTT TGAGTGAGTT GTTTAATACC 
CCTGCACATG TGAAACCAAG GAGAAGTGGT CGCTCAGGAG GCCTTCCGAT GGAAACTCCG 
CAATCTATAA TTGTTGATAT GTCACTGATG AAGACTCCAG AGGAATTAGG TGAAATGGTG 
GTTTCTCCAA TTAATGGTAG CCCTGCTACA ACACAGAAGA AACAGTACAG TCGTGACGCA 
GTATCTCGTC TTCTAAGTCC TGTGACGCCA GCATCAGAAG AACAGATATC CGAAGCTTCC 
AAAGACACTC AGTTGGTTGT AAAAGAGACA GCCACATTAA ATGAAACCCG TTTTATAAAA 
AAGGGTGCAA AGGCACAGAT GAAAAAAAGA GGAACAATGG GTTTGACTGG TGTAAAAAGG 
ATTATGAGAA CACCAAAACA AAAAGGAAAG CAGGTTATTG ATCCTGTGGC GCTAAAGAAA 
ATGTTGAGAA CACCAAAACA AGTTGAATCA CTTCCTGTAG AGTCCTCTGG CCCTAATGAT 
CTAGTTGGAC TGAAGGAAAT CATGAAAACT CCAAAAGTAA AAGGGCAACC AGTGGAGGAT 
ATGACTGGCA TAGCACGCAT CATGAAGACC CCAAGACGCA AGCAACAACC AGTAGAAGAT 
ATGGCTGGTA TTAAACGTAT TATGAAGACT CCTAAACAGA AAGGGACGCC TGTTGAGGAT 
ATGACCGGAA TCAAGCGTAT TATGACGACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGTAT TATGACAACC CCCAAGGAGA AAGGGCAACC CGTGGAGGAC 
ATGGTGGGAA TCAAACGCAT TATGAGAACC CCCAAGGAGA AAGGGCAACC TGTGGAGGAT 
ATGGAAGGTT TAAGTCAGAT TATGAGAACA CCAACTGAAA ATACTCACCC AATAGAAGAA 
ATATTTGGGA TGAAACAACT AATAAAGACT CCTCCAAAGA GAGCACTTGG TGTTAGAGAG 
GGCACGTCTG GGACACCATC ACAATCTGGC AAAAAGGCTA CTCTGTTTCA GACTAATCAA 
AAATCGGCAC AAGAAGAAGC ATTGCCAAAA CTTACTTTGG AGAAAAATAA TGAAAGCGAG 
GTTGCTGTAC AAACTGACAG AGAAACTGAA AAATCTAAAC CAACCCCCAA AAGAGGTCGA 
CCTGCCAAGG CTAGTGTGAA GCTTGCTTCA GATGGTGGGA AGCCTGTTGA TAATGGCACA 
TCTACACATG AAACACACCC AGAAGAAAGC AGCAGAGAGG CTTCTCTCCA AGTACTGGCT 
AGTGATGATA AATCCTCCAC AGCTGCTGAT AAAGCCACCC CAGCCAAACG AAGAGGTCGT 
CCATCCAAGT CCAGTTTGAG TGGTTCACCA GGTGGTGGAA ATTCTGTTGA AAACAACACA 
TTCAGACATG AAAAAAATCC GGAAGAAGCC GCTCCTCTAC AGGTACTCTC CACTGAAAAT 
ACTTCATCCT TGGGTGATGA TAAAGCTACT CCAGCTAAAA GAGGTCGTCC ATGCAAACGT 
GGTATGAGTA AGTCACCAGC ATCTAGTAAT AAAGATAAGT CTGTTGAAAA GAATAGTACA 
CCAGAGCATG AAAAGAACCT GGAAGAAAGC AATGAAGGAA CATCTCTACA GGTACAGACC 
ATTGAAGATG CTTCTTCCAC TAGTGCTGAT AAAGCCACTC CGGCCAAAAG AAGAGGTCGT 
CCAGCTAAAC GCAGCTTGAG TCATTCACCA GGTTTAGAAA AACCTGTTGT AGATGTTCCA 
GAAGACCTAA GCACCAGCAA TGAGGTCTCT AATATTCCTG AAGGATCAAG TCATATCAAT 
ACAGACCAAG AGAACAATGT TGATTTACCA ATGGAAAAAG AGTCTGAACC GTTAAATCTA 
AATATTGGAG CGAAAAAAGT CACTAAGAAA TCTCAGAGAA AGAAAGTCAC AGAAGAGTCT 
GTTCAAATAG CAGTGGAAAA CTTGGTTGTT GAACAAGATG ATGAAAAATC TGCTCCCCAA 
AGACGACGAG GAAGACCTCA AAGGGGTGGT CTTTCATCTA CCACTGAAGT TTTAGACGAA 
GCATTACCAG AAGAGCCTCC AACAAACAGA CGAACTGGCA GGGGCCGAAA GATTGCAGAA 
GTGGCAAAAC CGCAACAAGT CAGTACAAGT GTTGAAGAAT CTCAAGAAGT AAGAAACGAG 
GAACTCTCAG CAACTAAGAC TCAGACTAAG TCTACCAGGC ATCAAAAAGC TGTATCTGAA 
GACTCTACAG TTACAGCAAC TAAGAAGCCA CGTGGAAGGC AACCTAAAGT GGACAGAGAA 
CAGAAAGCTG CTGAGGAGGA AAATACTGTG GAGAAATCAC TTACAGAACT GCCAGGAGAT 
TCAGCTTTAG TCAACAATGT AACGCCATCT GTGAGAAGGG GTAGGCGAGC AAGAGGCAAT 
GCAAATGCTG TGACTGAAAC AGTCGTAACT GAAGCAAATT TGCCTGACTT GACAAACAAT 
ATCGTTTTAC ATAAAGAGCA AGAAAGCTCC AGTCAAGCAT CTCCAGAAGC CAAAACTCAA 
AGAGGAAAAA GGAAAAATCT TAAAAATACA AACGTTGATT CTGTAAGCAA CTCTGCTGAA 
ACAGATGTTG CAACATTAGA ATTAGAAGAG AGACCTAACA AAAGATCTAC ACGTGGTGTT 
AGAGGCACAC AGCAAGTTGA TGAGCTTAAA GAACCAAAAG GAAAAAAAGC AAAAGTAGAA 
CATGTGCCCA AACCTGCTGA ACTAGTTCAT ATACAGGAGA ATCTAGCTAA AGAGAATGCA 
TCTAGGGCGA AATCTGTGCA GTGGCACCCT CTTCTGACTG TTAAGGGGAC ACCTGAAAAG 
AATACTGAAG AGACTGTTAA GAATCTTGCA GATGAAGAAG CTCCGGTCAG TGCAGTAAAA 
TCTAGAGGAA GGAGGCGGCA AGAAGTAAAA GAATCTGTTC CAGCAAAACG ATCGCGTTCA 
AGAAGGGAGG AAAATAATGC AGAGGCATTA ACTTCTAGTA GTACAACAGC TGATGCAGTT 
CCTATTGCTT TGGTTGCCAG TGAAAGTACT GAAACTCCTA AGAATAAGAG AGGACGGAGG 
AAAGCAAACA CACAATTAGA GAAAGTTACT GATTCAGATC TTTCAGTGAC AGAGGTTTCC 
TCTAAGCTAG ACCTAGTGCC AGAGGTTGTA AACGAAAAGG CCGTAAACAA ACGAAACACA 
GGTAAAAGAA ACCTCAAGTC TTCATCTGAT AGTGATTCTA GTAGTAAAGC TGTTGAACCA 
GTGCTGGAGA CTATTACCCA AAAGCGTGGA CGAAGAGGAG CAGCAGCCAA AAGTCTAGAT 
TCTGAGACTA CTGAAGTTAA AACTGTTGAG TCTGCTGAAA ATGTAACTTC AAATAAAAGA 
AGGGGAAGAG CACCAAGTCA AAAAACCAAC AATCCTGAAA CTAGCAAAAC CACTACTGAA 
CCTGTTAATA AAAATCAAGC AACTGAAGCT GTAGCCAAAA ATACAGATAA TGCAGAAAGT 
CCTCCTAAAT CAAGAAGAGG CTTAAAAAGA AAGGCAGCCA CCACTGATGT TCCGGAAGGC 
AACACATCAG TAAGTGAAAA TGGACCCCGT GCCAATGTAA GAGAAAGCAC AAGAGGAGCT 
TCAAGGCAAA AAACTGCAGC AAAAGAAAAT CCAGTGGTTG TTGAAGAATC ACCAGCAAAG 
AAAAAGAGAA CAGAGCCATC TTCCAGTCCT GCTCAGAAAG GAGGAAAAAC AAGAGCCACT 
GCAGCAAAAC GGAAAAATGA GAAACCAACA GAAGTTGTAA AGACAAGGCA AACATTGCGC 
ACACGGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905888.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761748.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.

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CloneID	OXa62323
Clone ID Related Accession (Same CDS sequence)	XM_031905888.1
Accession Version	XM_031905888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCGGC CAAGAGGTCG 
CCGGCAAAGG GGGCATCCCC TGCCAAGAGG TCGCCGGCCA AGGCGGCATC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC ACCAGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTGA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761748.1
CDS	214..6912
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905888.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905888.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCGGC CAAGAGGTCG 
CCGGCAAAGG GGGCATCCCC TGCCAAGAGG TCGCCGGCCA AGGCGGCATC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC ACCAGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTGA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905889.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761749.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.

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CloneID	OXa62324
Clone ID Related Accession (Same CDS sequence)	XM_031905889.1
Accession Version	XM_031905889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGTAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCAAA GGCGGCATCC 
CCAGCCAAGA GGTCGCCGGC CAAGGCGGCT ACCCCGGCCA AGAGGTCGCC GGCAAAGGGG 
GCATCCCCTG CCAGGAGGTC GCCCGCCAAG GCAGCTACCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCGGC CAAGAGGTCG 
CCGGCAAAGG GGGCATCCCC TGCCAAGAGG TCGCCGGCCA AGGCGGCATC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC ACCAGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTGA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761749.1
CDS	214..6912
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905889.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905889.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGTAGC TACCCCGGCC 
AAGAGGTCGC CAGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCAAA GGCGGCATCC 
CCAGCCAAGA GGTCGCCGGC CAAGGCGGCT ACCCCGGCCA AGAGGTCGCC GGCAAAGGGG 
GCATCCCCTG CCAGGAGGTC GCCCGCCAAG GCAGCTACCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCAG CTACCCCGGC CAAGAGGTCG 
CCGGCAAAGG GGGCATCCCC TGCCAAGAGG TCGCCGGCCA AGGCGGCATC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAAGAGGTC GCCGGCCAAG 
GCGGCTACCC CGGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGGCG GCTACCCCGG CCAAGAGGTC ACCAGCCAAG 
GCAGCTACCC CTGCCAAGAG GTCGCCAGTC AAGGCAGCTA CCCCTGCCAA GAGGTCTCCT 
GCCTCAGCAA CCCCTGCCAA GAGGTCTCCT GCTTCTACTT ATACAAAAGG ACGTTTCTCA 
ATTTCACGAA TTGATACACC ACCGCAAATA GATGTCCATG CTCCTAACAC TGAGAAAAAT 
GAACTCTCTG CACAAACACC CAGAAAATCA CGGAAGTCAA TATCATTAAA GAAAACGCCA 
GGAAGAAGAA GTAGAAAACT TGAATCTTTT GAAATTTTGC GTTCCAGGCG AAGAAGTGGG 
GCAACTGAAG CTAACTTGCT TGTTGCAAAA TCATGGGCTG ATGTTGTAAG AAGCGGTGTT 
CCAAAAAATC AGAAGAAATC TGAAAAGCAT GCTCGCAAAG CAGTACCTAC CAAGAGAAAG 
CCAAAGCCAA AGACGCCAGC ACGAAGAATT ATTGACCGAA TTGGCACAGG ACATGCTGAT 
TCTCCTGCCA CAATCCTTGT TGGAAGAGCT CATACAAGAA CTGTCGACCT AACTGGATAT 
GTTCCTAAAA TTGTTCAAAA CCAAGCAATA AAAATCACCA ATGACCAAAA TGAAAGCTTT 
ACAGGTTTGA GTGAGTTGTT TAATACCCCT GCACATGTGA AACCAAGGAG AAGTGGTCGC 
TCAGGAGGCC TTCCGATGGA AACTCCGCAA TCTATAATTG TTGATATGTC ACTGATGAAG 
ACTCCAGAGG AATTAGGTGA AATGGTGGTT TCTCCAATTA ATGGTAGCCC TGCTACAACA 
CAGAAGAAAC AGTACAGTCG TGACGCAGTA TCTCGTCTTC TAAGTCCTGT GACGCCAGCA 
TCAGAAGAAC AGATATCCGA AGCTTCCAAA GACACTCAGT TGGTTGTAAA AGAGACAGCC 
ACATTAAATG AAACCCGTTT TATAAAAAAG GGTGCAAAGG CACAGATGAA AAAAAGAGGA 
ACAATGGGTT TGACTGGTGT AAAAAGGATT ATGAGAACAC CAAAACAAAA AGGAAAGCAG 
GTTATTGATC CTGTGGCGCT AAAGAAAATG TTGAGAACAC CAAAACAAGT TGAATCACTT 
CCTGTAGAGT CCTCTGGCCC TAATGATCTA GTTGGACTGA AGGAAATCAT GAAAACTCCA 
AAAGTAAAAG GGCAACCAGT GGAGGATATG ACTGGCATAG CACGCATCAT GAAGACCCCA 
AGACGCAAGC AACAACCAGT AGAAGATATG GCTGGTATTA AACGTATTAT GAAGACTCCT 
AAACAGAAAG GGACGCCTGT TGAGGATATG ACCGGAATCA AGCGTATTAT GACGACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGTATTAT GACAACCCCC 
AAGGAGAAAG GGCAACCCGT GGAGGACATG GTGGGAATCA AACGCATTAT GAGAACCCCC 
AAGGAGAAAG GGCAACCTGT GGAGGATATG GAAGGTTTAA GTCAGATTAT GAGAACACCA 
ACTGAAAATA CTCACCCAAT AGAAGAAATA TTTGGGATGA AACAACTAAT AAAGACTCCT 
CCAAAGAGAG CACTTGGTGT TAGAGAGGGC ACGTCTGGGA CACCATCACA ATCTGGCAAA 
AAGGCTACTC TGTTTCAGAC TAATCAAAAA TCGGCACAAG AAGAAGCATT GCCAAAACTT 
ACTTTGGAGA AAAATAATGA AAGCGAGGTT GCTGTACAAA CTGACAGAGA AACTGAAAAA 
TCTAAACCAA CCCCCAAAAG AGGTCGACCT GCCAAGGCTA GTGTGAAGCT TGCTTCAGAT 
GGTGGGAAGC CTGTTGATAA TGGCACATCT ACACATGAAA CACACCCAGA AGAAAGCAGC 
AGAGAGGCTT CTCTCCAAGT ACTGGCTAGT GATGATAAAT CCTCCACAGC TGCTGATAAA 
GCCACCCCAG CCAAACGAAG AGGTCGTCCA TCCAAGTCCA GTTTGAGTGG TTCACCAGGT 
GGTGGAAATT CTGTTGAAAA CAACACATTC AGACATGAAA AAAATCCGGA AGAAGCCGCT 
CCTCTACAGG TACTCTCCAC TGAAAATACT TCATCCTTGG GTGATGATAA AGCTACTCCA 
GCTAAAAGAG GTCGTCCATG CAAACGTGGT ATGAGTAAGT CACCAGCATC TAGTAATAAA 
GATAAGTCTG TTGAAAAGAA TAGTACACCA GAGCATGAAA AGAACCTGGA AGAAAGCAAT 
GAAGGAACAT CTCTACAGGT ACAGACCATT GAAGATGCTT CTTCCACTAG TGCTGATAAA 
GCCACTCCGG CCAAAAGAAG AGGTCGTCCA GCTAAACGCA GCTTGAGTCA TTCACCAGGT 
TTAGAAAAAC CTGTTGTAGA TGTTCCAGAA GACCTAAGCA CCAGCAATGA GGTCTCTAAT 
ATTCCTGAAG GATCAAGTCA TATCAATACA GACCAAGAGA ACAATGTTGA TTTACCAATG 
GAAAAAGAGT CTGAACCGTT AAATCTAAAT ATTGGAGCGA AAAAAGTCAC TAAGAAATCT 
CAGAGAAAGA AAGTCACAGA AGAGTCTGTT CAAATAGCAG TGGAAAACTT GGTTGTTGAA 
CAAGATGATG AAAAATCTGC TCCCCAAAGA CGACGAGGAA GACCTCAAAG GGGTGGTCTT 
TCATCTACCA CTGAAGTTTT AGACGAAGCA TTACCAGAAG AGCCTCCAAC AAACAGACGA 
ACTGGCAGGG GCCGAAAGAT TGCAGAAGTG GCAAAACCGC AACAAGTCAG TACAAGTGTT 
GAAGAATCTC AAGAAGTAAG AAACGAGGAA CTCTCAGCAA CTAAGACTCA GACTAAGTCT 
ACCAGGCATC AAAAAGCTGT ATCTGAAGAC TCTACAGTTA CAGCAACTAA GAAGCCACGT 
GGAAGGCAAC CTAAAGTGGA CAGAGAACAG AAAGCTGCTG AGGAGGAAAA TACTGTGGAG 
AAATCACTTA CAGAACTGCC AGGAGATTCA GCTTTAGTCA ACAATGTAAC GCCATCTGTG 
AGAAGGGGTA GGCGAGCAAG AGGCAATGCA AATGCTGTGA CTGAAACAGT CGTAACTGAA 
GCAAATTTGC CTGACTTGAC AAACAATATC GTTTTACATA AAGAGCAAGA AAGCTCCAGT 
CAAGCATCTC CAGAAGCCAA AACTCAAAGA GGAAAAAGGA AAAATCTTAA AAATACAAAC 
GTTGATTCTG TAAGCAACTC TGCTGAAACA GATGTTGCAA CATTAGAATT AGAAGAGAGA 
CCTAACAAAA GATCTACACG TGGTGTTAGA GGCACACAGC AAGTTGATGA GCTTAAAGAA 
CCAAAAGGAA AAAAAGCAAA AGTAGAACAT GTGCCCAAAC CTGCTGAACT AGTTCATATA 
CAGGAGAATC TAGCTAAAGA GAATGCATCT AGGGCGAAAT CTGTGCAGTG GCACCCTCTT 
CTGACTGTTA AGGGGACACC TGAAAAGAAT ACTGAAGAGA CTGTTAAGAA TCTTGCAGAT 
GAAGAAGCTC CGGTCAGTGC AGTAAAATCT AGAGGAAGGA GGCGGCAAGA AGTAAAAGAA 
TCTGTTCCAG CAAAACGATC GCGTTCAAGA AGGGAGGAAA ATAATGCAGA GGCATTAACT 
TCTAGTAGTA CAACAGCTGA TGCAGTTCCT ATTGCTTTGG TTGCCAGTGA AAGTACTGAA 
ACTCCTAAGA ATAAGAGAGG ACGGAGGAAA GCAAACACAC AATTAGAGAA AGTTACTGAT 
TCAGATCTTT CAGTGACAGA GGTTTCCTCT AAGCTAGACC TAGTGCCAGA GGTTGTAAAC 
GAAAAGGCCG TAAACAAACG AAACACAGGT AAAAGAAACC TCAAGTCTTC ATCTGATAGT 
GATTCTAGTA GTAAAGCTGT TGAACCAGTG CTGGAGACTA TTACCCAAAA GCGTGGACGA 
AGAGGAGCAG CAGCCAAAAG TCTAGATTCT GAGACTACTG AAGTTAAAAC TGTTGAGTCT 
GCTGAAAATG TAACTTCAAA TAAAAGAAGG GGAAGAGCAC CAAGTCAAAA AACCAACAAT 
CCTGAAACTA GCAAAACCAC TACTGAACCT GTTAATAAAA ATCAAGCAAC TGAAGCTGTA 
GCCAAAAATA CAGATAATGC AGAAAGTCCT CCTAAATCAA GAAGAGGCTT AAAAAGAAAG 
GCAGCCACCA CTGATGTTCC GGAAGGCAAC ACATCAGTAA GTGAAAATGG ACCCCGTGCC 
AATGTAAGAG AAAGCACAAG AGGAGCTTCA AGGCAAAAAA CTGCAGCAAA AGAAAATCCA 
GTGGTTGTTG AAGAATCACC AGCAAAGAAA AAGAGAACAG AGCCATCTTC CAGTCCTGCT 
CAGAAAGGAG GAAAAACAAG AGCCACTGCA GCAAAACGGA AAAATGAGAA ACCAACAGAA 
GTTGTAAAGA CAAGGCAAAC ATTGCGCACA CGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905890.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761750.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.

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CloneID	OXa62325
Clone ID Related Accession (Same CDS sequence)	XM_031905890.1 , XM_031905891.1
Accession Version	XM_031905890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCACCAG CCAAGGCAGC TACCCCTGCC 
AAGAGGTCGC CAGTCAAGGC AGCTACCCCT GCCAAGAGGT CTCCTGCCTC AGCAACCCCT 
GCCAAGAGGT CTCCTGCTTC TACTTATACA AAAGGACGTT TCTCAATTTC ACGAATTGAT 
ACACCACCGC AAATAGATGT CCATGCTCCT AACACTGAGA AAAATGAACT CTCTGCACAA 
ACACCCAGAA AATCACGGAA GTCAATATCA TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA 
AAACTTGAAT CTTTTGAAAT TTTGCGTTCC AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC 
TTGCTTGTTG CAAAATCATG GGCTGATGTT GTAAGAAGCG GTGTTCCAAA AAATCAGAAG 
AAATCTGAAA AGCATGCTCG CAAAGCAGTA CCTACCAAGA GAAAGCCAAA GCCAAAGACG 
CCAGCACGAA GAATTATTGA CCGAATTGGC ACAGGACATG CTGATTCTCC TGCCACAATC 
CTTGTTGGAA GAGCTCATAC AAGAACTGTC GACCTAACTG GATATGTTCC TAAAATTGTT 
CAAAACCAAG CAATAAAAAT CACCAATGAC CAAAATGAAA GCTTTACAGG TTTGAGTGAG 
TTGTTTAATA CCCCTGCACA TGTGAAACCA AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG 
ATGGAAACTC CGCAATCTAT AATTGTTGAT ATGTCACTGA TGAAGACTCC AGAGGAATTA 
GGTGAAATGG TGGTTTCTCC AATTAATGGT AGCCCTGCTA CAACACAGAA GAAACAGTAC 
AGTCGTGACG CAGTATCTCG TCTTCTAAGT CCTGTGACGC CAGCATCAGA AGAACAGATA 
TCCGAAGCTT CCAAAGACAC TCAGTTGGTT GTAAAAGAGA CAGCCACATT AAATGAAACC 
CGTTTTATAA AAAAGGGTGC AAAGGCACAG ATGAAAAAAA GAGGAACAAT GGGTTTGACT 
GGTGTAAAAA GGATTATGAG AACACCAAAA CAAAAAGGAA AGCAGGTTAT TGATCCTGTG 
GCGCTAAAGA AAATGTTGAG AACACCAAAA CAAGTTGAAT CACTTCCTGT AGAGTCCTCT 
GGCCCTAATG ATCTAGTTGG ACTGAAGGAA ATCATGAAAA CTCCAAAAGT AAAAGGGCAA 
CCAGTGGAGG ATATGACTGG CATAGCACGC ATCATGAAGA CCCCAAGACG CAAGCAACAA 
CCAGTAGAAG ATATGGCTGG TATTAAACGT ATTATGAAGA CTCCTAAACA GAAAGGGACG 
CCTGTTGAGG ATATGACCGG AATCAAGCGT ATTATGACGA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGT ATTATGACAA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGC ATTATGAGAA CCCCCAAGGA GAAAGGGCAA 
CCTGTGGAGG ATATGGAAGG TTTAAGTCAG ATTATGAGAA CACCAACTGA AAATACTCAC 
CCAATAGAAG AAATATTTGG GATGAAACAA CTAATAAAGA CTCCTCCAAA GAGAGCACTT 
GGTGTTAGAG AGGGCACGTC TGGGACACCA TCACAATCTG GCAAAAAGGC TACTCTGTTT 
CAGACTAATC AAAAATCGGC ACAAGAAGAA GCATTGCCAA AACTTACTTT GGAGAAAAAT 
AATGAAAGCG AGGTTGCTGT ACAAACTGAC AGAGAAACTG AAAAATCTAA ACCAACCCCC 
AAAAGAGGTC GACCTGCCAA GGCTAGTGTG AAGCTTGCTT CAGATGGTGG GAAGCCTGTT 
GATAATGGCA CATCTACACA TGAAACACAC CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC 
CAAGTACTGG CTAGTGATGA TAAATCCTCC ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA 
CGAAGAGGTC GTCCATCCAA GTCCAGTTTG AGTGGTTCAC CAGGTGGTGG AAATTCTGTT 
GAAAACAACA CATTCAGACA TGAAAAAAAT CCGGAAGAAG CCGCTCCTCT ACAGGTACTC 
TCCACTGAAA ATACTTCATC CTTGGGTGAT GATAAAGCTA CTCCAGCTAA AAGAGGTCGT 
CCATGCAAAC GTGGTATGAG TAAGTCACCA GCATCTAGTA ATAAAGATAA GTCTGTTGAA 
AAGAATAGTA CACCAGAGCA TGAAAAGAAC CTGGAAGAAA GCAATGAAGG AACATCTCTA 
CAGGTACAGA CCATTGAAGA TGCTTCTTCC ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA 
AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG AGTCATTCAC CAGGTTTAGA AAAACCTGTT 
GTAGATGTTC CAGAAGACCT AAGCACCAGC AATGAGGTCT CTAATATTCC TGAAGGATCA 
AGTCATATCA ATACAGACCA AGAGAACAAT GTTGATTTAC CAATGGAAAA AGAGTCTGAA 
CCGTTAAATC TAAATATTGG AGCGAAAAAA GTCACTAAGA AATCTCAGAG AAAGAAAGTC 
ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA AACTTGGTTG TTGAACAAGA TGATGAAAAA 
TCTGCTCCCC AAAGACGACG AGGAAGACCT CAAAGGGGTG GTCTTTCATC TACCACTGAA 
GTTTTAGACG AAGCATTACC AGAAGAGCCT CCAACAAACA GACGAACTGG CAGGGGCCGA 
AAGATTGCAG AAGTGGCAAA ACCGCAACAA GTCAGTACAA GTGTTGAAGA ATCTCAAGAA 
GTAAGAAACG AGGAACTCTC AGCAACTAAG ACTCAGACTA AGTCTACCAG GCATCAAAAA 
GCTGTATCTG AAGACTCTAC AGTTACAGCA ACTAAGAAGC CACGTGGAAG GCAACCTAAA 
GTGGACAGAG AACAGAAAGC TGCTGAGGAG GAAAATACTG TGGAGAAATC ACTTACAGAA 
CTGCCAGGAG ATTCAGCTTT AGTCAACAAT GTAACGCCAT CTGTGAGAAG GGGTAGGCGA 
GCAAGAGGCA ATGCAAATGC TGTGACTGAA ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC 
TTGACAAACA ATATCGTTTT ACATAAAGAG CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA 
GCCAAAACTC AAAGAGGAAA AAGGAAAAAT CTTAAAAATA CAAACGTTGA TTCTGTAAGC 
AACTCTGCTG AAACAGATGT TGCAACATTA GAATTAGAAG AGAGACCTAA CAAAAGATCT 
ACACGTGGTG TTAGAGGCAC ACAGCAAGTT GATGAGCTTA AAGAACCAAA AGGAAAAAAA 
GCAAAAGTAG AACATGTGCC CAAACCTGCT GAACTAGTTC ATATACAGGA GAATCTAGCT 
AAAGAGAATG CATCTAGGGC GAAATCTGTG CAGTGGCACC CTCTTCTGAC TGTTAAGGGG 
ACACCTGAAA AGAATACTGA AGAGACTGTT AAGAATCTTG CAGATGAAGA AGCTCCGGTC 
AGTGCAGTAA AATCTAGAGG AAGGAGGCGG CAAGAAGTAA AAGAATCTGT TCCAGCAAAA 
CGATCGCGTT CAAGAAGGGA GGAAAATAAT GCAGAGGCAT TAACTTCTAG TAGTACAACA 
GCTGATGCAG TTCCTATTGC TTTGGTTGCC AGTGAAAGTA CTGAAACTCC TAAGAATAAG 
AGAGGACGGA GGAAAGCAAA CACACAATTA GAGAAAGTTA CTGATTCAGA TCTTTCAGTG 
ACAGAGGTTT CCTCTAAGCT AGACCTAGTG CCAGAGGTTG TAAACGAAAA GGCCGTAAAC 
AAACGAAACA CAGGTAAAAG AAACCTCAAG TCTTCATCTG ATAGTGATTC TAGTAGTAAA 
GCTGTTGAAC CAGTGCTGGA GACTATTACC CAAAAGCGTG GACGAAGAGG AGCAGCAGCC 
AAAAGTCTAG ATTCTGAGAC TACTGAAGTT AAAACTGTTG AGTCTGCTGA AAATGTAACT 
TCAAATAAAA GAAGGGGAAG AGCACCAAGT CAAAAAACCA ACAATCCTGA AACTAGCAAA 
ACCACTACTG AACCTGTTAA TAAAAATCAA GCAACTGAAG CTGTAGCCAA AAATACAGAT 
AATGCAGAAA GTCCTCCTAA ATCAAGAAGA GGCTTAAAAA GAAAGGCAGC CACCACTGAT 
GTTCCGGAAG GCAACACATC AGTAAGTGAA AATGGACCCC GTGCCAATGT AAGAGAAAGC 
ACAAGAGGAG CTTCAAGGCA AAAAACTGCA GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA 
TCACCAGCAA AGAAAAAGAG AACAGAGCCA TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA 
ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT GAGAAACCAA CAGAAGTTGT AAAGACAAGG 
CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761750.1
CDS	214..6897
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905890.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905890.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCACCAG CCAAGGCAGC TACCCCTGCC 
AAGAGGTCGC CAGTCAAGGC AGCTACCCCT GCCAAGAGGT CTCCTGCCTC AGCAACCCCT 
GCCAAGAGGT CTCCTGCTTC TACTTATACA AAAGGACGTT TCTCAATTTC ACGAATTGAT 
ACACCACCGC AAATAGATGT CCATGCTCCT AACACTGAGA AAAATGAACT CTCTGCACAA 
ACACCCAGAA AATCACGGAA GTCAATATCA TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA 
AAACTTGAAT CTTTTGAAAT TTTGCGTTCC AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC 
TTGCTTGTTG CAAAATCATG GGCTGATGTT GTAAGAAGCG GTGTTCCAAA AAATCAGAAG 
AAATCTGAAA AGCATGCTCG CAAAGCAGTA CCTACCAAGA GAAAGCCAAA GCCAAAGACG 
CCAGCACGAA GAATTATTGA CCGAATTGGC ACAGGACATG CTGATTCTCC TGCCACAATC 
CTTGTTGGAA GAGCTCATAC AAGAACTGTC GACCTAACTG GATATGTTCC TAAAATTGTT 
CAAAACCAAG CAATAAAAAT CACCAATGAC CAAAATGAAA GCTTTACAGG TTTGAGTGAG 
TTGTTTAATA CCCCTGCACA TGTGAAACCA AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG 
ATGGAAACTC CGCAATCTAT AATTGTTGAT ATGTCACTGA TGAAGACTCC AGAGGAATTA 
GGTGAAATGG TGGTTTCTCC AATTAATGGT AGCCCTGCTA CAACACAGAA GAAACAGTAC 
AGTCGTGACG CAGTATCTCG TCTTCTAAGT CCTGTGACGC CAGCATCAGA AGAACAGATA 
TCCGAAGCTT CCAAAGACAC TCAGTTGGTT GTAAAAGAGA CAGCCACATT AAATGAAACC 
CGTTTTATAA AAAAGGGTGC AAAGGCACAG ATGAAAAAAA GAGGAACAAT GGGTTTGACT 
GGTGTAAAAA GGATTATGAG AACACCAAAA CAAAAAGGAA AGCAGGTTAT TGATCCTGTG 
GCGCTAAAGA AAATGTTGAG AACACCAAAA CAAGTTGAAT CACTTCCTGT AGAGTCCTCT 
GGCCCTAATG ATCTAGTTGG ACTGAAGGAA ATCATGAAAA CTCCAAAAGT AAAAGGGCAA 
CCAGTGGAGG ATATGACTGG CATAGCACGC ATCATGAAGA CCCCAAGACG CAAGCAACAA 
CCAGTAGAAG ATATGGCTGG TATTAAACGT ATTATGAAGA CTCCTAAACA GAAAGGGACG 
CCTGTTGAGG ATATGACCGG AATCAAGCGT ATTATGACGA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGT ATTATGACAA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGC ATTATGAGAA CCCCCAAGGA GAAAGGGCAA 
CCTGTGGAGG ATATGGAAGG TTTAAGTCAG ATTATGAGAA CACCAACTGA AAATACTCAC 
CCAATAGAAG AAATATTTGG GATGAAACAA CTAATAAAGA CTCCTCCAAA GAGAGCACTT 
GGTGTTAGAG AGGGCACGTC TGGGACACCA TCACAATCTG GCAAAAAGGC TACTCTGTTT 
CAGACTAATC AAAAATCGGC ACAAGAAGAA GCATTGCCAA AACTTACTTT GGAGAAAAAT 
AATGAAAGCG AGGTTGCTGT ACAAACTGAC AGAGAAACTG AAAAATCTAA ACCAACCCCC 
AAAAGAGGTC GACCTGCCAA GGCTAGTGTG AAGCTTGCTT CAGATGGTGG GAAGCCTGTT 
GATAATGGCA CATCTACACA TGAAACACAC CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC 
CAAGTACTGG CTAGTGATGA TAAATCCTCC ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA 
CGAAGAGGTC GTCCATCCAA GTCCAGTTTG AGTGGTTCAC CAGGTGGTGG AAATTCTGTT 
GAAAACAACA CATTCAGACA TGAAAAAAAT CCGGAAGAAG CCGCTCCTCT ACAGGTACTC 
TCCACTGAAA ATACTTCATC CTTGGGTGAT GATAAAGCTA CTCCAGCTAA AAGAGGTCGT 
CCATGCAAAC GTGGTATGAG TAAGTCACCA GCATCTAGTA ATAAAGATAA GTCTGTTGAA 
AAGAATAGTA CACCAGAGCA TGAAAAGAAC CTGGAAGAAA GCAATGAAGG AACATCTCTA 
CAGGTACAGA CCATTGAAGA TGCTTCTTCC ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA 
AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG AGTCATTCAC CAGGTTTAGA AAAACCTGTT 
GTAGATGTTC CAGAAGACCT AAGCACCAGC AATGAGGTCT CTAATATTCC TGAAGGATCA 
AGTCATATCA ATACAGACCA AGAGAACAAT GTTGATTTAC CAATGGAAAA AGAGTCTGAA 
CCGTTAAATC TAAATATTGG AGCGAAAAAA GTCACTAAGA AATCTCAGAG AAAGAAAGTC 
ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA AACTTGGTTG TTGAACAAGA TGATGAAAAA 
TCTGCTCCCC AAAGACGACG AGGAAGACCT CAAAGGGGTG GTCTTTCATC TACCACTGAA 
GTTTTAGACG AAGCATTACC AGAAGAGCCT CCAACAAACA GACGAACTGG CAGGGGCCGA 
AAGATTGCAG AAGTGGCAAA ACCGCAACAA GTCAGTACAA GTGTTGAAGA ATCTCAAGAA 
GTAAGAAACG AGGAACTCTC AGCAACTAAG ACTCAGACTA AGTCTACCAG GCATCAAAAA 
GCTGTATCTG AAGACTCTAC AGTTACAGCA ACTAAGAAGC CACGTGGAAG GCAACCTAAA 
GTGGACAGAG AACAGAAAGC TGCTGAGGAG GAAAATACTG TGGAGAAATC ACTTACAGAA 
CTGCCAGGAG ATTCAGCTTT AGTCAACAAT GTAACGCCAT CTGTGAGAAG GGGTAGGCGA 
GCAAGAGGCA ATGCAAATGC TGTGACTGAA ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC 
TTGACAAACA ATATCGTTTT ACATAAAGAG CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA 
GCCAAAACTC AAAGAGGAAA AAGGAAAAAT CTTAAAAATA CAAACGTTGA TTCTGTAAGC 
AACTCTGCTG AAACAGATGT TGCAACATTA GAATTAGAAG AGAGACCTAA CAAAAGATCT 
ACACGTGGTG TTAGAGGCAC ACAGCAAGTT GATGAGCTTA AAGAACCAAA AGGAAAAAAA 
GCAAAAGTAG AACATGTGCC CAAACCTGCT GAACTAGTTC ATATACAGGA GAATCTAGCT 
AAAGAGAATG CATCTAGGGC GAAATCTGTG CAGTGGCACC CTCTTCTGAC TGTTAAGGGG 
ACACCTGAAA AGAATACTGA AGAGACTGTT AAGAATCTTG CAGATGAAGA AGCTCCGGTC 
AGTGCAGTAA AATCTAGAGG AAGGAGGCGG CAAGAAGTAA AAGAATCTGT TCCAGCAAAA 
CGATCGCGTT CAAGAAGGGA GGAAAATAAT GCAGAGGCAT TAACTTCTAG TAGTACAACA 
GCTGATGCAG TTCCTATTGC TTTGGTTGCC AGTGAAAGTA CTGAAACTCC TAAGAATAAG 
AGAGGACGGA GGAAAGCAAA CACACAATTA GAGAAAGTTA CTGATTCAGA TCTTTCAGTG 
ACAGAGGTTT CCTCTAAGCT AGACCTAGTG CCAGAGGTTG TAAACGAAAA GGCCGTAAAC 
AAACGAAACA CAGGTAAAAG AAACCTCAAG TCTTCATCTG ATAGTGATTC TAGTAGTAAA 
GCTGTTGAAC CAGTGCTGGA GACTATTACC CAAAAGCGTG GACGAAGAGG AGCAGCAGCC 
AAAAGTCTAG ATTCTGAGAC TACTGAAGTT AAAACTGTTG AGTCTGCTGA AAATGTAACT 
TCAAATAAAA GAAGGGGAAG AGCACCAAGT CAAAAAACCA ACAATCCTGA AACTAGCAAA 
ACCACTACTG AACCTGTTAA TAAAAATCAA GCAACTGAAG CTGTAGCCAA AAATACAGAT 
AATGCAGAAA GTCCTCCTAA ATCAAGAAGA GGCTTAAAAA GAAAGGCAGC CACCACTGAT 
GTTCCGGAAG GCAACACATC AGTAAGTGAA AATGGACCCC GTGCCAATGT AAGAGAAAGC 
ACAAGAGGAG CTTCAAGGCA AAAAACTGCA GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA 
TCACCAGCAA AGAAAAAGAG AACAGAGCCA TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA 
ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT GAGAAACCAA CAGAAGTTGT AAAGACAAGG 
CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905891.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761751.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.

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CloneID	OXa62325
Clone ID Related Accession (Same CDS sequence)	XM_031905890.1 , XM_031905891.1
Accession Version	XM_031905891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCACCAG CCAAGGCAGC TACCCCTGCC 
AAGAGGTCGC CAGTCAAGGC AGCTACCCCT GCCAAGAGGT CTCCTGCCTC AGCAACCCCT 
GCCAAGAGGT CTCCTGCTTC TACTTATACA AAAGGACGTT TCTCAATTTC ACGAATTGAT 
ACACCACCGC AAATAGATGT CCATGCTCCT AACACTGAGA AAAATGAACT CTCTGCACAA 
ACACCCAGAA AATCACGGAA GTCAATATCA TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA 
AAACTTGAAT CTTTTGAAAT TTTGCGTTCC AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC 
TTGCTTGTTG CAAAATCATG GGCTGATGTT GTAAGAAGCG GTGTTCCAAA AAATCAGAAG 
AAATCTGAAA AGCATGCTCG CAAAGCAGTA CCTACCAAGA GAAAGCCAAA GCCAAAGACG 
CCAGCACGAA GAATTATTGA CCGAATTGGC ACAGGACATG CTGATTCTCC TGCCACAATC 
CTTGTTGGAA GAGCTCATAC AAGAACTGTC GACCTAACTG GATATGTTCC TAAAATTGTT 
CAAAACCAAG CAATAAAAAT CACCAATGAC CAAAATGAAA GCTTTACAGG TTTGAGTGAG 
TTGTTTAATA CCCCTGCACA TGTGAAACCA AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG 
ATGGAAACTC CGCAATCTAT AATTGTTGAT ATGTCACTGA TGAAGACTCC AGAGGAATTA 
GGTGAAATGG TGGTTTCTCC AATTAATGGT AGCCCTGCTA CAACACAGAA GAAACAGTAC 
AGTCGTGACG CAGTATCTCG TCTTCTAAGT CCTGTGACGC CAGCATCAGA AGAACAGATA 
TCCGAAGCTT CCAAAGACAC TCAGTTGGTT GTAAAAGAGA CAGCCACATT AAATGAAACC 
CGTTTTATAA AAAAGGGTGC AAAGGCACAG ATGAAAAAAA GAGGAACAAT GGGTTTGACT 
GGTGTAAAAA GGATTATGAG AACACCAAAA CAAAAAGGAA AGCAGGTTAT TGATCCTGTG 
GCGCTAAAGA AAATGTTGAG AACACCAAAA CAAGTTGAAT CACTTCCTGT AGAGTCCTCT 
GGCCCTAATG ATCTAGTTGG ACTGAAGGAA ATCATGAAAA CTCCAAAAGT AAAAGGGCAA 
CCAGTGGAGG ATATGACTGG CATAGCACGC ATCATGAAGA CCCCAAGACG CAAGCAACAA 
CCAGTAGAAG ATATGGCTGG TATTAAACGT ATTATGAAGA CTCCTAAACA GAAAGGGACG 
CCTGTTGAGG ATATGACCGG AATCAAGCGT ATTATGACGA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGT ATTATGACAA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGC ATTATGAGAA CCCCCAAGGA GAAAGGGCAA 
CCTGTGGAGG ATATGGAAGG TTTAAGTCAG ATTATGAGAA CACCAACTGA AAATACTCAC 
CCAATAGAAG AAATATTTGG GATGAAACAA CTAATAAAGA CTCCTCCAAA GAGAGCACTT 
GGTGTTAGAG AGGGCACGTC TGGGACACCA TCACAATCTG GCAAAAAGGC TACTCTGTTT 
CAGACTAATC AAAAATCGGC ACAAGAAGAA GCATTGCCAA AACTTACTTT GGAGAAAAAT 
AATGAAAGCG AGGTTGCTGT ACAAACTGAC AGAGAAACTG AAAAATCTAA ACCAACCCCC 
AAAAGAGGTC GACCTGCCAA GGCTAGTGTG AAGCTTGCTT CAGATGGTGG GAAGCCTGTT 
GATAATGGCA CATCTACACA TGAAACACAC CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC 
CAAGTACTGG CTAGTGATGA TAAATCCTCC ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA 
CGAAGAGGTC GTCCATCCAA GTCCAGTTTG AGTGGTTCAC CAGGTGGTGG AAATTCTGTT 
GAAAACAACA CATTCAGACA TGAAAAAAAT CCGGAAGAAG CCGCTCCTCT ACAGGTACTC 
TCCACTGAAA ATACTTCATC CTTGGGTGAT GATAAAGCTA CTCCAGCTAA AAGAGGTCGT 
CCATGCAAAC GTGGTATGAG TAAGTCACCA GCATCTAGTA ATAAAGATAA GTCTGTTGAA 
AAGAATAGTA CACCAGAGCA TGAAAAGAAC CTGGAAGAAA GCAATGAAGG AACATCTCTA 
CAGGTACAGA CCATTGAAGA TGCTTCTTCC ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA 
AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG AGTCATTCAC CAGGTTTAGA AAAACCTGTT 
GTAGATGTTC CAGAAGACCT AAGCACCAGC AATGAGGTCT CTAATATTCC TGAAGGATCA 
AGTCATATCA ATACAGACCA AGAGAACAAT GTTGATTTAC CAATGGAAAA AGAGTCTGAA 
CCGTTAAATC TAAATATTGG AGCGAAAAAA GTCACTAAGA AATCTCAGAG AAAGAAAGTC 
ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA AACTTGGTTG TTGAACAAGA TGATGAAAAA 
TCTGCTCCCC AAAGACGACG AGGAAGACCT CAAAGGGGTG GTCTTTCATC TACCACTGAA 
GTTTTAGACG AAGCATTACC AGAAGAGCCT CCAACAAACA GACGAACTGG CAGGGGCCGA 
AAGATTGCAG AAGTGGCAAA ACCGCAACAA GTCAGTACAA GTGTTGAAGA ATCTCAAGAA 
GTAAGAAACG AGGAACTCTC AGCAACTAAG ACTCAGACTA AGTCTACCAG GCATCAAAAA 
GCTGTATCTG AAGACTCTAC AGTTACAGCA ACTAAGAAGC CACGTGGAAG GCAACCTAAA 
GTGGACAGAG AACAGAAAGC TGCTGAGGAG GAAAATACTG TGGAGAAATC ACTTACAGAA 
CTGCCAGGAG ATTCAGCTTT AGTCAACAAT GTAACGCCAT CTGTGAGAAG GGGTAGGCGA 
GCAAGAGGCA ATGCAAATGC TGTGACTGAA ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC 
TTGACAAACA ATATCGTTTT ACATAAAGAG CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA 
GCCAAAACTC AAAGAGGAAA AAGGAAAAAT CTTAAAAATA CAAACGTTGA TTCTGTAAGC 
AACTCTGCTG AAACAGATGT TGCAACATTA GAATTAGAAG AGAGACCTAA CAAAAGATCT 
ACACGTGGTG TTAGAGGCAC ACAGCAAGTT GATGAGCTTA AAGAACCAAA AGGAAAAAAA 
GCAAAAGTAG AACATGTGCC CAAACCTGCT GAACTAGTTC ATATACAGGA GAATCTAGCT 
AAAGAGAATG CATCTAGGGC GAAATCTGTG CAGTGGCACC CTCTTCTGAC TGTTAAGGGG 
ACACCTGAAA AGAATACTGA AGAGACTGTT AAGAATCTTG CAGATGAAGA AGCTCCGGTC 
AGTGCAGTAA AATCTAGAGG AAGGAGGCGG CAAGAAGTAA AAGAATCTGT TCCAGCAAAA 
CGATCGCGTT CAAGAAGGGA GGAAAATAAT GCAGAGGCAT TAACTTCTAG TAGTACAACA 
GCTGATGCAG TTCCTATTGC TTTGGTTGCC AGTGAAAGTA CTGAAACTCC TAAGAATAAG 
AGAGGACGGA GGAAAGCAAA CACACAATTA GAGAAAGTTA CTGATTCAGA TCTTTCAGTG 
ACAGAGGTTT CCTCTAAGCT AGACCTAGTG CCAGAGGTTG TAAACGAAAA GGCCGTAAAC 
AAACGAAACA CAGGTAAAAG AAACCTCAAG TCTTCATCTG ATAGTGATTC TAGTAGTAAA 
GCTGTTGAAC CAGTGCTGGA GACTATTACC CAAAAGCGTG GACGAAGAGG AGCAGCAGCC 
AAAAGTCTAG ATTCTGAGAC TACTGAAGTT AAAACTGTTG AGTCTGCTGA AAATGTAACT 
TCAAATAAAA GAAGGGGAAG AGCACCAAGT CAAAAAACCA ACAATCCTGA AACTAGCAAA 
ACCACTACTG AACCTGTTAA TAAAAATCAA GCAACTGAAG CTGTAGCCAA AAATACAGAT 
AATGCAGAAA GTCCTCCTAA ATCAAGAAGA GGCTTAAAAA GAAAGGCAGC CACCACTGAT 
GTTCCGGAAG GCAACACATC AGTAAGTGAA AATGGACCCC GTGCCAATGT AAGAGAAAGC 
ACAAGAGGAG CTTCAAGGCA AAAAACTGCA GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA 
TCACCAGCAA AGAAAAAGAG AACAGAGCCA TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA 
ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT GAGAAACCAA CAGAAGTTGT AAAGACAAGG 
CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761751.1
CDS	214..6897
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905891.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905891.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCACCAG CCAAGGCAGC TACCCCTGCC 
AAGAGGTCGC CAGTCAAGGC AGCTACCCCT GCCAAGAGGT CTCCTGCCTC AGCAACCCCT 
GCCAAGAGGT CTCCTGCTTC TACTTATACA AAAGGACGTT TCTCAATTTC ACGAATTGAT 
ACACCACCGC AAATAGATGT CCATGCTCCT AACACTGAGA AAAATGAACT CTCTGCACAA 
ACACCCAGAA AATCACGGAA GTCAATATCA TTAAAGAAAA CGCCAGGAAG AAGAAGTAGA 
AAACTTGAAT CTTTTGAAAT TTTGCGTTCC AGGCGAAGAA GTGGGGCAAC TGAAGCTAAC 
TTGCTTGTTG CAAAATCATG GGCTGATGTT GTAAGAAGCG GTGTTCCAAA AAATCAGAAG 
AAATCTGAAA AGCATGCTCG CAAAGCAGTA CCTACCAAGA GAAAGCCAAA GCCAAAGACG 
CCAGCACGAA GAATTATTGA CCGAATTGGC ACAGGACATG CTGATTCTCC TGCCACAATC 
CTTGTTGGAA GAGCTCATAC AAGAACTGTC GACCTAACTG GATATGTTCC TAAAATTGTT 
CAAAACCAAG CAATAAAAAT CACCAATGAC CAAAATGAAA GCTTTACAGG TTTGAGTGAG 
TTGTTTAATA CCCCTGCACA TGTGAAACCA AGGAGAAGTG GTCGCTCAGG AGGCCTTCCG 
ATGGAAACTC CGCAATCTAT AATTGTTGAT ATGTCACTGA TGAAGACTCC AGAGGAATTA 
GGTGAAATGG TGGTTTCTCC AATTAATGGT AGCCCTGCTA CAACACAGAA GAAACAGTAC 
AGTCGTGACG CAGTATCTCG TCTTCTAAGT CCTGTGACGC CAGCATCAGA AGAACAGATA 
TCCGAAGCTT CCAAAGACAC TCAGTTGGTT GTAAAAGAGA CAGCCACATT AAATGAAACC 
CGTTTTATAA AAAAGGGTGC AAAGGCACAG ATGAAAAAAA GAGGAACAAT GGGTTTGACT 
GGTGTAAAAA GGATTATGAG AACACCAAAA CAAAAAGGAA AGCAGGTTAT TGATCCTGTG 
GCGCTAAAGA AAATGTTGAG AACACCAAAA CAAGTTGAAT CACTTCCTGT AGAGTCCTCT 
GGCCCTAATG ATCTAGTTGG ACTGAAGGAA ATCATGAAAA CTCCAAAAGT AAAAGGGCAA 
CCAGTGGAGG ATATGACTGG CATAGCACGC ATCATGAAGA CCCCAAGACG CAAGCAACAA 
CCAGTAGAAG ATATGGCTGG TATTAAACGT ATTATGAAGA CTCCTAAACA GAAAGGGACG 
CCTGTTGAGG ATATGACCGG AATCAAGCGT ATTATGACGA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGT ATTATGACAA CCCCCAAGGA GAAAGGGCAA 
CCCGTGGAGG ACATGGTGGG AATCAAACGC ATTATGAGAA CCCCCAAGGA GAAAGGGCAA 
CCTGTGGAGG ATATGGAAGG TTTAAGTCAG ATTATGAGAA CACCAACTGA AAATACTCAC 
CCAATAGAAG AAATATTTGG GATGAAACAA CTAATAAAGA CTCCTCCAAA GAGAGCACTT 
GGTGTTAGAG AGGGCACGTC TGGGACACCA TCACAATCTG GCAAAAAGGC TACTCTGTTT 
CAGACTAATC AAAAATCGGC ACAAGAAGAA GCATTGCCAA AACTTACTTT GGAGAAAAAT 
AATGAAAGCG AGGTTGCTGT ACAAACTGAC AGAGAAACTG AAAAATCTAA ACCAACCCCC 
AAAAGAGGTC GACCTGCCAA GGCTAGTGTG AAGCTTGCTT CAGATGGTGG GAAGCCTGTT 
GATAATGGCA CATCTACACA TGAAACACAC CCAGAAGAAA GCAGCAGAGA GGCTTCTCTC 
CAAGTACTGG CTAGTGATGA TAAATCCTCC ACAGCTGCTG ATAAAGCCAC CCCAGCCAAA 
CGAAGAGGTC GTCCATCCAA GTCCAGTTTG AGTGGTTCAC CAGGTGGTGG AAATTCTGTT 
GAAAACAACA CATTCAGACA TGAAAAAAAT CCGGAAGAAG CCGCTCCTCT ACAGGTACTC 
TCCACTGAAA ATACTTCATC CTTGGGTGAT GATAAAGCTA CTCCAGCTAA AAGAGGTCGT 
CCATGCAAAC GTGGTATGAG TAAGTCACCA GCATCTAGTA ATAAAGATAA GTCTGTTGAA 
AAGAATAGTA CACCAGAGCA TGAAAAGAAC CTGGAAGAAA GCAATGAAGG AACATCTCTA 
CAGGTACAGA CCATTGAAGA TGCTTCTTCC ACTAGTGCTG ATAAAGCCAC TCCGGCCAAA 
AGAAGAGGTC GTCCAGCTAA ACGCAGCTTG AGTCATTCAC CAGGTTTAGA AAAACCTGTT 
GTAGATGTTC CAGAAGACCT AAGCACCAGC AATGAGGTCT CTAATATTCC TGAAGGATCA 
AGTCATATCA ATACAGACCA AGAGAACAAT GTTGATTTAC CAATGGAAAA AGAGTCTGAA 
CCGTTAAATC TAAATATTGG AGCGAAAAAA GTCACTAAGA AATCTCAGAG AAAGAAAGTC 
ACAGAAGAGT CTGTTCAAAT AGCAGTGGAA AACTTGGTTG TTGAACAAGA TGATGAAAAA 
TCTGCTCCCC AAAGACGACG AGGAAGACCT CAAAGGGGTG GTCTTTCATC TACCACTGAA 
GTTTTAGACG AAGCATTACC AGAAGAGCCT CCAACAAACA GACGAACTGG CAGGGGCCGA 
AAGATTGCAG AAGTGGCAAA ACCGCAACAA GTCAGTACAA GTGTTGAAGA ATCTCAAGAA 
GTAAGAAACG AGGAACTCTC AGCAACTAAG ACTCAGACTA AGTCTACCAG GCATCAAAAA 
GCTGTATCTG AAGACTCTAC AGTTACAGCA ACTAAGAAGC CACGTGGAAG GCAACCTAAA 
GTGGACAGAG AACAGAAAGC TGCTGAGGAG GAAAATACTG TGGAGAAATC ACTTACAGAA 
CTGCCAGGAG ATTCAGCTTT AGTCAACAAT GTAACGCCAT CTGTGAGAAG GGGTAGGCGA 
GCAAGAGGCA ATGCAAATGC TGTGACTGAA ACAGTCGTAA CTGAAGCAAA TTTGCCTGAC 
TTGACAAACA ATATCGTTTT ACATAAAGAG CAAGAAAGCT CCAGTCAAGC ATCTCCAGAA 
GCCAAAACTC AAAGAGGAAA AAGGAAAAAT CTTAAAAATA CAAACGTTGA TTCTGTAAGC 
AACTCTGCTG AAACAGATGT TGCAACATTA GAATTAGAAG AGAGACCTAA CAAAAGATCT 
ACACGTGGTG TTAGAGGCAC ACAGCAAGTT GATGAGCTTA AAGAACCAAA AGGAAAAAAA 
GCAAAAGTAG AACATGTGCC CAAACCTGCT GAACTAGTTC ATATACAGGA GAATCTAGCT 
AAAGAGAATG CATCTAGGGC GAAATCTGTG CAGTGGCACC CTCTTCTGAC TGTTAAGGGG 
ACACCTGAAA AGAATACTGA AGAGACTGTT AAGAATCTTG CAGATGAAGA AGCTCCGGTC 
AGTGCAGTAA AATCTAGAGG AAGGAGGCGG CAAGAAGTAA AAGAATCTGT TCCAGCAAAA 
CGATCGCGTT CAAGAAGGGA GGAAAATAAT GCAGAGGCAT TAACTTCTAG TAGTACAACA 
GCTGATGCAG TTCCTATTGC TTTGGTTGCC AGTGAAAGTA CTGAAACTCC TAAGAATAAG 
AGAGGACGGA GGAAAGCAAA CACACAATTA GAGAAAGTTA CTGATTCAGA TCTTTCAGTG 
ACAGAGGTTT CCTCTAAGCT AGACCTAGTG CCAGAGGTTG TAAACGAAAA GGCCGTAAAC 
AAACGAAACA CAGGTAAAAG AAACCTCAAG TCTTCATCTG ATAGTGATTC TAGTAGTAAA 
GCTGTTGAAC CAGTGCTGGA GACTATTACC CAAAAGCGTG GACGAAGAGG AGCAGCAGCC 
AAAAGTCTAG ATTCTGAGAC TACTGAAGTT AAAACTGTTG AGTCTGCTGA AAATGTAACT 
TCAAATAAAA GAAGGGGAAG AGCACCAAGT CAAAAAACCA ACAATCCTGA AACTAGCAAA 
ACCACTACTG AACCTGTTAA TAAAAATCAA GCAACTGAAG CTGTAGCCAA AAATACAGAT 
AATGCAGAAA GTCCTCCTAA ATCAAGAAGA GGCTTAAAAA GAAAGGCAGC CACCACTGAT 
GTTCCGGAAG GCAACACATC AGTAAGTGAA AATGGACCCC GTGCCAATGT AAGAGAAAGC 
ACAAGAGGAG CTTCAAGGCA AAAAACTGCA GCAAAAGAAA ATCCAGTGGT TGTTGAAGAA 
TCACCAGCAA AGAAAAAGAG AACAGAGCCA TCTTCCAGTC CTGCTCAGAA AGGAGGAAAA 
ACAAGAGCCA CTGCAGCAAA ACGGAAAAAT GAGAAACCAA CAGAAGTTGT AAAGACAAGG 
CAAACATTGC GCACACGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905892.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761752.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.

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CloneID	OXa62326
Clone ID Related Accession (Same CDS sequence)	XM_031905892.1
Accession Version	XM_031905892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCACCAGCCA AGGCAGCTAC CCCTGCCAAG AGGTCGCCAG TCAAGGCAGC TACCCCTGCC 
AAGAGGTCTC CTGCCTCAGC AACCCCTGCC AAGAGGTCTC CTGCTTCTAC TTATACAAAA 
GGACGTTTCT CAATTTCACG AATTGATACA CCACCGCAAA TAGATGTCCA TGCTCCTAAC 
ACTGAGAAAA ATGAACTCTC TGCACAAACA CCCAGAAAAT CACGGAAGTC AATATCATTA 
AAGAAAACGC CAGGAAGAAG AAGTAGAAAA CTTGAATCTT TTGAAATTTT GCGTTCCAGG 
CGAAGAAGTG GGGCAACTGA AGCTAACTTG CTTGTTGCAA AATCATGGGC TGATGTTGTA 
AGAAGCGGTG TTCCAAAAAA TCAGAAGAAA TCTGAAAAGC ATGCTCGCAA AGCAGTACCT 
ACCAAGAGAA AGCCAAAGCC AAAGACGCCA GCACGAAGAA TTATTGACCG AATTGGCACA 
GGACATGCTG ATTCTCCTGC CACAATCCTT GTTGGAAGAG CTCATACAAG AACTGTCGAC 
CTAACTGGAT ATGTTCCTAA AATTGTTCAA AACCAAGCAA TAAAAATCAC CAATGACCAA 
AATGAAAGCT TTACAGGTTT GAGTGAGTTG TTTAATACCC CTGCACATGT GAAACCAAGG 
AGAAGTGGTC GCTCAGGAGG CCTTCCGATG GAAACTCCGC AATCTATAAT TGTTGATATG 
TCACTGATGA AGACTCCAGA GGAATTAGGT GAAATGGTGG TTTCTCCAAT TAATGGTAGC 
CCTGCTACAA CACAGAAGAA ACAGTACAGT CGTGACGCAG TATCTCGTCT TCTAAGTCCT 
GTGACGCCAG CATCAGAAGA ACAGATATCC GAAGCTTCCA AAGACACTCA GTTGGTTGTA 
AAAGAGACAG CCACATTAAA TGAAACCCGT TTTATAAAAA AGGGTGCAAA GGCACAGATG 
AAAAAAAGAG GAACAATGGG TTTGACTGGT GTAAAAAGGA TTATGAGAAC ACCAAAACAA 
AAAGGAAAGC AGGTTATTGA TCCTGTGGCG CTAAAGAAAA TGTTGAGAAC ACCAAAACAA 
GTTGAATCAC TTCCTGTAGA GTCCTCTGGC CCTAATGATC TAGTTGGACT GAAGGAAATC 
ATGAAAACTC CAAAAGTAAA AGGGCAACCA GTGGAGGATA TGACTGGCAT AGCACGCATC 
ATGAAGACCC CAAGACGCAA GCAACAACCA GTAGAAGATA TGGCTGGTAT TAAACGTATT 
ATGAAGACTC CTAAACAGAA AGGGACGCCT GTTGAGGATA TGACCGGAAT CAAGCGTATT 
ATGACGACCC CCAAGGAGAA AGGGCAACCC GTGGAGGACA TGGTGGGAAT CAAACGTATT 
ATGACAACCC CCAAGGAGAA AGGGCAACCC GTGGAGGACA TGGTGGGAAT CAAACGCATT 
ATGAGAACCC CCAAGGAGAA AGGGCAACCT GTGGAGGATA TGGAAGGTTT AAGTCAGATT 
ATGAGAACAC CAACTGAAAA TACTCACCCA ATAGAAGAAA TATTTGGGAT GAAACAACTA 
ATAAAGACTC CTCCAAAGAG AGCACTTGGT GTTAGAGAGG GCACGTCTGG GACACCATCA 
CAATCTGGCA AAAAGGCTAC TCTGTTTCAG ACTAATCAAA AATCGGCACA AGAAGAAGCA 
TTGCCAAAAC TTACTTTGGA GAAAAATAAT GAAAGCGAGG TTGCTGTACA AACTGACAGA 
GAAACTGAAA AATCTAAACC AACCCCCAAA AGAGGTCGAC CTGCCAAGGC TAGTGTGAAG 
CTTGCTTCAG ATGGTGGGAA GCCTGTTGAT AATGGCACAT CTACACATGA AACACACCCA 
GAAGAAAGCA GCAGAGAGGC TTCTCTCCAA GTACTGGCTA GTGATGATAA ATCCTCCACA 
GCTGCTGATA AAGCCACCCC AGCCAAACGA AGAGGTCGTC CATCCAAGTC CAGTTTGAGT 
GGTTCACCAG GTGGTGGAAA TTCTGTTGAA AACAACACAT TCAGACATGA AAAAAATCCG 
GAAGAAGCCG CTCCTCTACA GGTACTCTCC ACTGAAAATA CTTCATCCTT GGGTGATGAT 
AAAGCTACTC CAGCTAAAAG AGGTCGTCCA TGCAAACGTG GTATGAGTAA GTCACCAGCA 
TCTAGTAATA AAGATAAGTC TGTTGAAAAG AATAGTACAC CAGAGCATGA AAAGAACCTG 
GAAGAAAGCA ATGAAGGAAC ATCTCTACAG GTACAGACCA TTGAAGATGC TTCTTCCACT 
AGTGCTGATA AAGCCACTCC GGCCAAAAGA AGAGGTCGTC CAGCTAAACG CAGCTTGAGT 
CATTCACCAG GTTTAGAAAA ACCTGTTGTA GATGTTCCAG AAGACCTAAG CACCAGCAAT 
GAGGTCTCTA ATATTCCTGA AGGATCAAGT CATATCAATA CAGACCAAGA GAACAATGTT 
GATTTACCAA TGGAAAAAGA GTCTGAACCG TTAAATCTAA ATATTGGAGC GAAAAAAGTC 
ACTAAGAAAT CTCAGAGAAA GAAAGTCACA GAAGAGTCTG TTCAAATAGC AGTGGAAAAC 
TTGGTTGTTG AACAAGATGA TGAAAAATCT GCTCCCCAAA GACGACGAGG AAGACCTCAA 
AGGGGTGGTC TTTCATCTAC CACTGAAGTT TTAGACGAAG CATTACCAGA AGAGCCTCCA 
ACAAACAGAC GAACTGGCAG GGGCCGAAAG ATTGCAGAAG TGGCAAAACC GCAACAAGTC 
AGTACAAGTG TTGAAGAATC TCAAGAAGTA AGAAACGAGG AACTCTCAGC AACTAAGACT 
CAGACTAAGT CTACCAGGCA TCAAAAAGCT GTATCTGAAG ACTCTACAGT TACAGCAACT 
AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG GACAGAGAAC AGAAAGCTGC TGAGGAGGAA 
AATACTGTGG AGAAATCACT TACAGAACTG CCAGGAGATT CAGCTTTAGT CAACAATGTA 
ACGCCATCTG TGAGAAGGGG TAGGCGAGCA AGAGGCAATG CAAATGCTGT GACTGAAACA 
GTCGTAACTG AAGCAAATTT GCCTGACTTG ACAAACAATA TCGTTTTACA TAAAGAGCAA 
GAAAGCTCCA GTCAAGCATC TCCAGAAGCC AAAACTCAAA GAGGAAAAAG GAAAAATCTT 
AAAAATACAA ACGTTGATTC TGTAAGCAAC TCTGCTGAAA CAGATGTTGC AACATTAGAA 
TTAGAAGAGA GACCTAACAA AAGATCTACA CGTGGTGTTA GAGGCACACA GCAAGTTGAT 
GAGCTTAAAG AACCAAAAGG AAAAAAAGCA AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA 
CTAGTTCATA TACAGGAGAA TCTAGCTAAA GAGAATGCAT CTAGGGCGAA ATCTGTGCAG 
TGGCACCCTC TTCTGACTGT TAAGGGGACA CCTGAAAAGA ATACTGAAGA GACTGTTAAG 
AATCTTGCAG ATGAAGAAGC TCCGGTCAGT GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA 
GAAGTAAAAG AATCTGTTCC AGCAAAACGA TCGCGTTCAA GAAGGGAGGA AAATAATGCA 
GAGGCATTAA CTTCTAGTAG TACAACAGCT GATGCAGTTC CTATTGCTTT GGTTGCCAGT 
GAAAGTACTG AAACTCCTAA GAATAAGAGA GGACGGAGGA AAGCAAACAC ACAATTAGAG 
AAAGTTACTG ATTCAGATCT TTCAGTGACA GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA 
GAGGTTGTAA ACGAAAAGGC CGTAAACAAA CGAAACACAG GTAAAAGAAA CCTCAAGTCT 
TCATCTGATA GTGATTCTAG TAGTAAAGCT GTTGAACCAG TGCTGGAGAC TATTACCCAA 
AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA AGTCTAGATT CTGAGACTAC TGAAGTTAAA 
ACTGTTGAGT CTGCTGAAAA TGTAACTTCA AATAAAAGAA GGGGAAGAGC ACCAAGTCAA 
AAAACCAACA ATCCTGAAAC TAGCAAAACC ACTACTGAAC CTGTTAATAA AAATCAAGCA 
ACTGAAGCTG TAGCCAAAAA TACAGATAAT GCAGAAAGTC CTCCTAAATC AAGAAGAGGC 
TTAAAAAGAA AGGCAGCCAC CACTGATGTT CCGGAAGGCA ACACATCAGT AAGTGAAAAT 
GGACCCCGTG CCAATGTAAG AGAAAGCACA AGAGGAGCTT CAAGGCAAAA AACTGCAGCA 
AAAGAAAATC CAGTGGTTGT TGAAGAATCA CCAGCAAAGA AAAAGAGAAC AGAGCCATCT 
TCCAGTCCTG CTCAGAAAGG AGGAAAAACA AGAGCCACTG CAGCAAAACG GAAAAATGAG 
AAACCAACAG AAGTTGTAAA GACAAGGCAA ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761752.1
CDS	214..6864
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905892.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905892.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCG CCGGCCAAGG CGGCTACCCC GGCCAAGAGG 
TCACCAGCCA AGGCAGCTAC CCCTGCCAAG AGGTCGCCAG TCAAGGCAGC TACCCCTGCC 
AAGAGGTCTC CTGCCTCAGC AACCCCTGCC AAGAGGTCTC CTGCTTCTAC TTATACAAAA 
GGACGTTTCT CAATTTCACG AATTGATACA CCACCGCAAA TAGATGTCCA TGCTCCTAAC 
ACTGAGAAAA ATGAACTCTC TGCACAAACA CCCAGAAAAT CACGGAAGTC AATATCATTA 
AAGAAAACGC CAGGAAGAAG AAGTAGAAAA CTTGAATCTT TTGAAATTTT GCGTTCCAGG 
CGAAGAAGTG GGGCAACTGA AGCTAACTTG CTTGTTGCAA AATCATGGGC TGATGTTGTA 
AGAAGCGGTG TTCCAAAAAA TCAGAAGAAA TCTGAAAAGC ATGCTCGCAA AGCAGTACCT 
ACCAAGAGAA AGCCAAAGCC AAAGACGCCA GCACGAAGAA TTATTGACCG AATTGGCACA 
GGACATGCTG ATTCTCCTGC CACAATCCTT GTTGGAAGAG CTCATACAAG AACTGTCGAC 
CTAACTGGAT ATGTTCCTAA AATTGTTCAA AACCAAGCAA TAAAAATCAC CAATGACCAA 
AATGAAAGCT TTACAGGTTT GAGTGAGTTG TTTAATACCC CTGCACATGT GAAACCAAGG 
AGAAGTGGTC GCTCAGGAGG CCTTCCGATG GAAACTCCGC AATCTATAAT TGTTGATATG 
TCACTGATGA AGACTCCAGA GGAATTAGGT GAAATGGTGG TTTCTCCAAT TAATGGTAGC 
CCTGCTACAA CACAGAAGAA ACAGTACAGT CGTGACGCAG TATCTCGTCT TCTAAGTCCT 
GTGACGCCAG CATCAGAAGA ACAGATATCC GAAGCTTCCA AAGACACTCA GTTGGTTGTA 
AAAGAGACAG CCACATTAAA TGAAACCCGT TTTATAAAAA AGGGTGCAAA GGCACAGATG 
AAAAAAAGAG GAACAATGGG TTTGACTGGT GTAAAAAGGA TTATGAGAAC ACCAAAACAA 
AAAGGAAAGC AGGTTATTGA TCCTGTGGCG CTAAAGAAAA TGTTGAGAAC ACCAAAACAA 
GTTGAATCAC TTCCTGTAGA GTCCTCTGGC CCTAATGATC TAGTTGGACT GAAGGAAATC 
ATGAAAACTC CAAAAGTAAA AGGGCAACCA GTGGAGGATA TGACTGGCAT AGCACGCATC 
ATGAAGACCC CAAGACGCAA GCAACAACCA GTAGAAGATA TGGCTGGTAT TAAACGTATT 
ATGAAGACTC CTAAACAGAA AGGGACGCCT GTTGAGGATA TGACCGGAAT CAAGCGTATT 
ATGACGACCC CCAAGGAGAA AGGGCAACCC GTGGAGGACA TGGTGGGAAT CAAACGTATT 
ATGACAACCC CCAAGGAGAA AGGGCAACCC GTGGAGGACA TGGTGGGAAT CAAACGCATT 
ATGAGAACCC CCAAGGAGAA AGGGCAACCT GTGGAGGATA TGGAAGGTTT AAGTCAGATT 
ATGAGAACAC CAACTGAAAA TACTCACCCA ATAGAAGAAA TATTTGGGAT GAAACAACTA 
ATAAAGACTC CTCCAAAGAG AGCACTTGGT GTTAGAGAGG GCACGTCTGG GACACCATCA 
CAATCTGGCA AAAAGGCTAC TCTGTTTCAG ACTAATCAAA AATCGGCACA AGAAGAAGCA 
TTGCCAAAAC TTACTTTGGA GAAAAATAAT GAAAGCGAGG TTGCTGTACA AACTGACAGA 
GAAACTGAAA AATCTAAACC AACCCCCAAA AGAGGTCGAC CTGCCAAGGC TAGTGTGAAG 
CTTGCTTCAG ATGGTGGGAA GCCTGTTGAT AATGGCACAT CTACACATGA AACACACCCA 
GAAGAAAGCA GCAGAGAGGC TTCTCTCCAA GTACTGGCTA GTGATGATAA ATCCTCCACA 
GCTGCTGATA AAGCCACCCC AGCCAAACGA AGAGGTCGTC CATCCAAGTC CAGTTTGAGT 
GGTTCACCAG GTGGTGGAAA TTCTGTTGAA AACAACACAT TCAGACATGA AAAAAATCCG 
GAAGAAGCCG CTCCTCTACA GGTACTCTCC ACTGAAAATA CTTCATCCTT GGGTGATGAT 
AAAGCTACTC CAGCTAAAAG AGGTCGTCCA TGCAAACGTG GTATGAGTAA GTCACCAGCA 
TCTAGTAATA AAGATAAGTC TGTTGAAAAG AATAGTACAC CAGAGCATGA AAAGAACCTG 
GAAGAAAGCA ATGAAGGAAC ATCTCTACAG GTACAGACCA TTGAAGATGC TTCTTCCACT 
AGTGCTGATA AAGCCACTCC GGCCAAAAGA AGAGGTCGTC CAGCTAAACG CAGCTTGAGT 
CATTCACCAG GTTTAGAAAA ACCTGTTGTA GATGTTCCAG AAGACCTAAG CACCAGCAAT 
GAGGTCTCTA ATATTCCTGA AGGATCAAGT CATATCAATA CAGACCAAGA GAACAATGTT 
GATTTACCAA TGGAAAAAGA GTCTGAACCG TTAAATCTAA ATATTGGAGC GAAAAAAGTC 
ACTAAGAAAT CTCAGAGAAA GAAAGTCACA GAAGAGTCTG TTCAAATAGC AGTGGAAAAC 
TTGGTTGTTG AACAAGATGA TGAAAAATCT GCTCCCCAAA GACGACGAGG AAGACCTCAA 
AGGGGTGGTC TTTCATCTAC CACTGAAGTT TTAGACGAAG CATTACCAGA AGAGCCTCCA 
ACAAACAGAC GAACTGGCAG GGGCCGAAAG ATTGCAGAAG TGGCAAAACC GCAACAAGTC 
AGTACAAGTG TTGAAGAATC TCAAGAAGTA AGAAACGAGG AACTCTCAGC AACTAAGACT 
CAGACTAAGT CTACCAGGCA TCAAAAAGCT GTATCTGAAG ACTCTACAGT TACAGCAACT 
AAGAAGCCAC GTGGAAGGCA ACCTAAAGTG GACAGAGAAC AGAAAGCTGC TGAGGAGGAA 
AATACTGTGG AGAAATCACT TACAGAACTG CCAGGAGATT CAGCTTTAGT CAACAATGTA 
ACGCCATCTG TGAGAAGGGG TAGGCGAGCA AGAGGCAATG CAAATGCTGT GACTGAAACA 
GTCGTAACTG AAGCAAATTT GCCTGACTTG ACAAACAATA TCGTTTTACA TAAAGAGCAA 
GAAAGCTCCA GTCAAGCATC TCCAGAAGCC AAAACTCAAA GAGGAAAAAG GAAAAATCTT 
AAAAATACAA ACGTTGATTC TGTAAGCAAC TCTGCTGAAA CAGATGTTGC AACATTAGAA 
TTAGAAGAGA GACCTAACAA AAGATCTACA CGTGGTGTTA GAGGCACACA GCAAGTTGAT 
GAGCTTAAAG AACCAAAAGG AAAAAAAGCA AAAGTAGAAC ATGTGCCCAA ACCTGCTGAA 
CTAGTTCATA TACAGGAGAA TCTAGCTAAA GAGAATGCAT CTAGGGCGAA ATCTGTGCAG 
TGGCACCCTC TTCTGACTGT TAAGGGGACA CCTGAAAAGA ATACTGAAGA GACTGTTAAG 
AATCTTGCAG ATGAAGAAGC TCCGGTCAGT GCAGTAAAAT CTAGAGGAAG GAGGCGGCAA 
GAAGTAAAAG AATCTGTTCC AGCAAAACGA TCGCGTTCAA GAAGGGAGGA AAATAATGCA 
GAGGCATTAA CTTCTAGTAG TACAACAGCT GATGCAGTTC CTATTGCTTT GGTTGCCAGT 
GAAAGTACTG AAACTCCTAA GAATAAGAGA GGACGGAGGA AAGCAAACAC ACAATTAGAG 
AAAGTTACTG ATTCAGATCT TTCAGTGACA GAGGTTTCCT CTAAGCTAGA CCTAGTGCCA 
GAGGTTGTAA ACGAAAAGGC CGTAAACAAA CGAAACACAG GTAAAAGAAA CCTCAAGTCT 
TCATCTGATA GTGATTCTAG TAGTAAAGCT GTTGAACCAG TGCTGGAGAC TATTACCCAA 
AAGCGTGGAC GAAGAGGAGC AGCAGCCAAA AGTCTAGATT CTGAGACTAC TGAAGTTAAA 
ACTGTTGAGT CTGCTGAAAA TGTAACTTCA AATAAAAGAA GGGGAAGAGC ACCAAGTCAA 
AAAACCAACA ATCCTGAAAC TAGCAAAACC ACTACTGAAC CTGTTAATAA AAATCAAGCA 
ACTGAAGCTG TAGCCAAAAA TACAGATAAT GCAGAAAGTC CTCCTAAATC AAGAAGAGGC 
TTAAAAAGAA AGGCAGCCAC CACTGATGTT CCGGAAGGCA ACACATCAGT AAGTGAAAAT 
GGACCCCGTG CCAATGTAAG AGAAAGCACA AGAGGAGCTT CAAGGCAAAA AACTGCAGCA 
AAAGAAAATC CAGTGGTTGT TGAAGAATCA CCAGCAAAGA AAAAGAGAAC AGAGCCATCT 
TCCAGTCCTG CTCAGAAAGG AGGAAAAACA AGAGCCACTG CAGCAAAACG GAAAAATGAG 
AAACCAACAG AAGTTGTAAA GACAAGGCAA ACATTGCGCA CACGGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905893.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761753.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.

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CloneID	OXa62327
Clone ID Related Accession (Same CDS sequence)	XM_031905893.1
Accession Version	XM_031905893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCA CCAGCCAAGG CAGCTACCCC TGCCAAGAGG 
TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG 
AGGTCTCCTG CTTCTACTTA TACAAAAGGA CGTTTCTCAA TTTCACGAAT TGATACACCA 
CCGCAAATAG ATGTCCATGC TCCTAACACT GAGAAAAATG AACTCTCTGC ACAAACACCC 
AGAAAATCAC GGAAGTCAAT ATCATTAAAG AAAACGCCAG GAAGAAGAAG TAGAAAACTT 
GAATCTTTTG AAATTTTGCG TTCCAGGCGA AGAAGTGGGG CAACTGAAGC TAACTTGCTT 
GTTGCAAAAT CATGGGCTGA TGTTGTAAGA AGCGGTGTTC CAAAAAATCA GAAGAAATCT 
GAAAAGCATG CTCGCAAAGC AGTACCTACC AAGAGAAAGC CAAAGCCAAA GACGCCAGCA 
CGAAGAATTA TTGACCGAAT TGGCACAGGA CATGCTGATT CTCCTGCCAC AATCCTTGTT 
GGAAGAGCTC ATACAAGAAC TGTCGACCTA ACTGGATATG TTCCTAAAAT TGTTCAAAAC 
CAAGCAATAA AAATCACCAA TGACCAAAAT GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT 
AATACCCCTG CACATGTGAA ACCAAGGAGA AGTGGTCGCT CAGGAGGCCT TCCGATGGAA 
ACTCCGCAAT CTATAATTGT TGATATGTCA CTGATGAAGA CTCCAGAGGA ATTAGGTGAA 
ATGGTGGTTT CTCCAATTAA TGGTAGCCCT GCTACAACAC AGAAGAAACA GTACAGTCGT 
GACGCAGTAT CTCGTCTTCT AAGTCCTGTG ACGCCAGCAT CAGAAGAACA GATATCCGAA 
GCTTCCAAAG ACACTCAGTT GGTTGTAAAA GAGACAGCCA CATTAAATGA AACCCGTTTT 
ATAAAAAAGG GTGCAAAGGC ACAGATGAAA AAAAGAGGAA CAATGGGTTT GACTGGTGTA 
AAAAGGATTA TGAGAACACC AAAACAAAAA GGAAAGCAGG TTATTGATCC TGTGGCGCTA 
AAGAAAATGT TGAGAACACC AAAACAAGTT GAATCACTTC CTGTAGAGTC CTCTGGCCCT 
AATGATCTAG TTGGACTGAA GGAAATCATG AAAACTCCAA AAGTAAAAGG GCAACCAGTG 
GAGGATATGA CTGGCATAGC ACGCATCATG AAGACCCCAA GACGCAAGCA ACAACCAGTA 
GAAGATATGG CTGGTATTAA ACGTATTATG AAGACTCCTA AACAGAAAGG GACGCCTGTT 
GAGGATATGA CCGGAATCAA GCGTATTATG ACGACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGTATTATG ACAACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGCATTATG AGAACCCCCA AGGAGAAAGG GCAACCTGTG 
GAGGATATGG AAGGTTTAAG TCAGATTATG AGAACACCAA CTGAAAATAC TCACCCAATA 
GAAGAAATAT TTGGGATGAA ACAACTAATA AAGACTCCTC CAAAGAGAGC ACTTGGTGTT 
AGAGAGGGCA CGTCTGGGAC ACCATCACAA TCTGGCAAAA AGGCTACTCT GTTTCAGACT 
AATCAAAAAT CGGCACAAGA AGAAGCATTG CCAAAACTTA CTTTGGAGAA AAATAATGAA 
AGCGAGGTTG CTGTACAAAC TGACAGAGAA ACTGAAAAAT CTAAACCAAC CCCCAAAAGA 
GGTCGACCTG CCAAGGCTAG TGTGAAGCTT GCTTCAGATG GTGGGAAGCC TGTTGATAAT 
GGCACATCTA CACATGAAAC ACACCCAGAA GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA 
CTGGCTAGTG ATGATAAATC CTCCACAGCT GCTGATAAAG CCACCCCAGC CAAACGAAGA 
GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT TCACCAGGTG GTGGAAATTC TGTTGAAAAC 
AACACATTCA GACATGAAAA AAATCCGGAA GAAGCCGCTC CTCTACAGGT ACTCTCCACT 
GAAAATACTT CATCCTTGGG TGATGATAAA GCTACTCCAG CTAAAAGAGG TCGTCCATGC 
AAACGTGGTA TGAGTAAGTC ACCAGCATCT AGTAATAAAG ATAAGTCTGT TGAAAAGAAT 
AGTACACCAG AGCATGAAAA GAACCTGGAA GAAAGCAATG AAGGAACATC TCTACAGGTA 
CAGACCATTG AAGATGCTTC TTCCACTAGT GCTGATAAAG CCACTCCGGC CAAAAGAAGA 
GGTCGTCCAG CTAAACGCAG CTTGAGTCAT TCACCAGGTT TAGAAAAACC TGTTGTAGAT 
GTTCCAGAAG ACCTAAGCAC CAGCAATGAG GTCTCTAATA TTCCTGAAGG ATCAAGTCAT 
ATCAATACAG ACCAAGAGAA CAATGTTGAT TTACCAATGG AAAAAGAGTC TGAACCGTTA 
AATCTAAATA TTGGAGCGAA AAAAGTCACT AAGAAATCTC AGAGAAAGAA AGTCACAGAA 
GAGTCTGTTC AAATAGCAGT GGAAAACTTG GTTGTTGAAC AAGATGATGA AAAATCTGCT 
CCCCAAAGAC GACGAGGAAG ACCTCAAAGG GGTGGTCTTT CATCTACCAC TGAAGTTTTA 
GACGAAGCAT TACCAGAAGA GCCTCCAACA AACAGACGAA CTGGCAGGGG CCGAAAGATT 
GCAGAAGTGG CAAAACCGCA ACAAGTCAGT ACAAGTGTTG AAGAATCTCA AGAAGTAAGA 
AACGAGGAAC TCTCAGCAAC TAAGACTCAG ACTAAGTCTA CCAGGCATCA AAAAGCTGTA 
TCTGAAGACT CTACAGTTAC AGCAACTAAG AAGCCACGTG GAAGGCAACC TAAAGTGGAC 
AGAGAACAGA AAGCTGCTGA GGAGGAAAAT ACTGTGGAGA AATCACTTAC AGAACTGCCA 
GGAGATTCAG CTTTAGTCAA CAATGTAACG CCATCTGTGA GAAGGGGTAG GCGAGCAAGA 
GGCAATGCAA ATGCTGTGAC TGAAACAGTC GTAACTGAAG CAAATTTGCC TGACTTGACA 
AACAATATCG TTTTACATAA AGAGCAAGAA AGCTCCAGTC AAGCATCTCC AGAAGCCAAA 
ACTCAAAGAG GAAAAAGGAA AAATCTTAAA AATACAAACG TTGATTCTGT AAGCAACTCT 
GCTGAAACAG ATGTTGCAAC ATTAGAATTA GAAGAGAGAC CTAACAAAAG ATCTACACGT 
GGTGTTAGAG GCACACAGCA AGTTGATGAG CTTAAAGAAC CAAAAGGAAA AAAAGCAAAA 
GTAGAACATG TGCCCAAACC TGCTGAACTA GTTCATATAC AGGAGAATCT AGCTAAAGAG 
AATGCATCTA GGGCGAAATC TGTGCAGTGG CACCCTCTTC TGACTGTTAA GGGGACACCT 
GAAAAGAATA CTGAAGAGAC TGTTAAGAAT CTTGCAGATG AAGAAGCTCC GGTCAGTGCA 
GTAAAATCTA GAGGAAGGAG GCGGCAAGAA GTAAAAGAAT CTGTTCCAGC AAAACGATCG 
CGTTCAAGAA GGGAGGAAAA TAATGCAGAG GCATTAACTT CTAGTAGTAC AACAGCTGAT 
GCAGTTCCTA TTGCTTTGGT TGCCAGTGAA AGTACTGAAA CTCCTAAGAA TAAGAGAGGA 
CGGAGGAAAG CAAACACACA ATTAGAGAAA GTTACTGATT CAGATCTTTC AGTGACAGAG 
GTTTCCTCTA AGCTAGACCT AGTGCCAGAG GTTGTAAACG AAAAGGCCGT AAACAAACGA 
AACACAGGTA AAAGAAACCT CAAGTCTTCA TCTGATAGTG ATTCTAGTAG TAAAGCTGTT 
GAACCAGTGC TGGAGACTAT TACCCAAAAG CGTGGACGAA GAGGAGCAGC AGCCAAAAGT 
CTAGATTCTG AGACTACTGA AGTTAAAACT GTTGAGTCTG CTGAAAATGT AACTTCAAAT 
AAAAGAAGGG GAAGAGCACC AAGTCAAAAA ACCAACAATC CTGAAACTAG CAAAACCACT 
ACTGAACCTG TTAATAAAAA TCAAGCAACT GAAGCTGTAG CCAAAAATAC AGATAATGCA 
GAAAGTCCTC CTAAATCAAG AAGAGGCTTA AAAAGAAAGG CAGCCACCAC TGATGTTCCG 
GAAGGCAACA CATCAGTAAG TGAAAATGGA CCCCGTGCCA ATGTAAGAGA AAGCACAAGA 
GGAGCTTCAA GGCAAAAAAC TGCAGCAAAA GAAAATCCAG TGGTTGTTGA AGAATCACCA 
GCAAAGAAAA AGAGAACAGA GCCATCTTCC AGTCCTGCTC AGAAAGGAGG AAAAACAAGA 
GCCACTGCAG CAAAACGGAA AAATGAGAAA CCAACAGAAG TTGTAAAGAC AAGGCAAACA 
TTGCGCACAC GGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761753.1
CDS	214..6831
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905893.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905893.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAGGAGGT CGCCGGCCAA GGCGGCTACC CCTGCCAGGA GGTCGCCGGC CAAGGCGGCT 
ACCCCTGCCA GGAGGTCGCC GGCCAAGGCG GCTACCCCTG CCAGGAGGTC GCCGGCCAAG 
GCGGCTACCC CTGCCAAGAG GTCGCCGGCC AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG 
GCCAAGGCGG CTACCCCGGC CAAGAGGTCA CCAGCCAAGG CAGCTACCCC TGCCAAGAGG 
TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG 
AGGTCTCCTG CTTCTACTTA TACAAAAGGA CGTTTCTCAA TTTCACGAAT TGATACACCA 
CCGCAAATAG ATGTCCATGC TCCTAACACT GAGAAAAATG AACTCTCTGC ACAAACACCC 
AGAAAATCAC GGAAGTCAAT ATCATTAAAG AAAACGCCAG GAAGAAGAAG TAGAAAACTT 
GAATCTTTTG AAATTTTGCG TTCCAGGCGA AGAAGTGGGG CAACTGAAGC TAACTTGCTT 
GTTGCAAAAT CATGGGCTGA TGTTGTAAGA AGCGGTGTTC CAAAAAATCA GAAGAAATCT 
GAAAAGCATG CTCGCAAAGC AGTACCTACC AAGAGAAAGC CAAAGCCAAA GACGCCAGCA 
CGAAGAATTA TTGACCGAAT TGGCACAGGA CATGCTGATT CTCCTGCCAC AATCCTTGTT 
GGAAGAGCTC ATACAAGAAC TGTCGACCTA ACTGGATATG TTCCTAAAAT TGTTCAAAAC 
CAAGCAATAA AAATCACCAA TGACCAAAAT GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT 
AATACCCCTG CACATGTGAA ACCAAGGAGA AGTGGTCGCT CAGGAGGCCT TCCGATGGAA 
ACTCCGCAAT CTATAATTGT TGATATGTCA CTGATGAAGA CTCCAGAGGA ATTAGGTGAA 
ATGGTGGTTT CTCCAATTAA TGGTAGCCCT GCTACAACAC AGAAGAAACA GTACAGTCGT 
GACGCAGTAT CTCGTCTTCT AAGTCCTGTG ACGCCAGCAT CAGAAGAACA GATATCCGAA 
GCTTCCAAAG ACACTCAGTT GGTTGTAAAA GAGACAGCCA CATTAAATGA AACCCGTTTT 
ATAAAAAAGG GTGCAAAGGC ACAGATGAAA AAAAGAGGAA CAATGGGTTT GACTGGTGTA 
AAAAGGATTA TGAGAACACC AAAACAAAAA GGAAAGCAGG TTATTGATCC TGTGGCGCTA 
AAGAAAATGT TGAGAACACC AAAACAAGTT GAATCACTTC CTGTAGAGTC CTCTGGCCCT 
AATGATCTAG TTGGACTGAA GGAAATCATG AAAACTCCAA AAGTAAAAGG GCAACCAGTG 
GAGGATATGA CTGGCATAGC ACGCATCATG AAGACCCCAA GACGCAAGCA ACAACCAGTA 
GAAGATATGG CTGGTATTAA ACGTATTATG AAGACTCCTA AACAGAAAGG GACGCCTGTT 
GAGGATATGA CCGGAATCAA GCGTATTATG ACGACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGTATTATG ACAACCCCCA AGGAGAAAGG GCAACCCGTG 
GAGGACATGG TGGGAATCAA ACGCATTATG AGAACCCCCA AGGAGAAAGG GCAACCTGTG 
GAGGATATGG AAGGTTTAAG TCAGATTATG AGAACACCAA CTGAAAATAC TCACCCAATA 
GAAGAAATAT TTGGGATGAA ACAACTAATA AAGACTCCTC CAAAGAGAGC ACTTGGTGTT 
AGAGAGGGCA CGTCTGGGAC ACCATCACAA TCTGGCAAAA AGGCTACTCT GTTTCAGACT 
AATCAAAAAT CGGCACAAGA AGAAGCATTG CCAAAACTTA CTTTGGAGAA AAATAATGAA 
AGCGAGGTTG CTGTACAAAC TGACAGAGAA ACTGAAAAAT CTAAACCAAC CCCCAAAAGA 
GGTCGACCTG CCAAGGCTAG TGTGAAGCTT GCTTCAGATG GTGGGAAGCC TGTTGATAAT 
GGCACATCTA CACATGAAAC ACACCCAGAA GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA 
CTGGCTAGTG ATGATAAATC CTCCACAGCT GCTGATAAAG CCACCCCAGC CAAACGAAGA 
GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT TCACCAGGTG GTGGAAATTC TGTTGAAAAC 
AACACATTCA GACATGAAAA AAATCCGGAA GAAGCCGCTC CTCTACAGGT ACTCTCCACT 
GAAAATACTT CATCCTTGGG TGATGATAAA GCTACTCCAG CTAAAAGAGG TCGTCCATGC 
AAACGTGGTA TGAGTAAGTC ACCAGCATCT AGTAATAAAG ATAAGTCTGT TGAAAAGAAT 
AGTACACCAG AGCATGAAAA GAACCTGGAA GAAAGCAATG AAGGAACATC TCTACAGGTA 
CAGACCATTG AAGATGCTTC TTCCACTAGT GCTGATAAAG CCACTCCGGC CAAAAGAAGA 
GGTCGTCCAG CTAAACGCAG CTTGAGTCAT TCACCAGGTT TAGAAAAACC TGTTGTAGAT 
GTTCCAGAAG ACCTAAGCAC CAGCAATGAG GTCTCTAATA TTCCTGAAGG ATCAAGTCAT 
ATCAATACAG ACCAAGAGAA CAATGTTGAT TTACCAATGG AAAAAGAGTC TGAACCGTTA 
AATCTAAATA TTGGAGCGAA AAAAGTCACT AAGAAATCTC AGAGAAAGAA AGTCACAGAA 
GAGTCTGTTC AAATAGCAGT GGAAAACTTG GTTGTTGAAC AAGATGATGA AAAATCTGCT 
CCCCAAAGAC GACGAGGAAG ACCTCAAAGG GGTGGTCTTT CATCTACCAC TGAAGTTTTA 
GACGAAGCAT TACCAGAAGA GCCTCCAACA AACAGACGAA CTGGCAGGGG CCGAAAGATT 
GCAGAAGTGG CAAAACCGCA ACAAGTCAGT ACAAGTGTTG AAGAATCTCA AGAAGTAAGA 
AACGAGGAAC TCTCAGCAAC TAAGACTCAG ACTAAGTCTA CCAGGCATCA AAAAGCTGTA 
TCTGAAGACT CTACAGTTAC AGCAACTAAG AAGCCACGTG GAAGGCAACC TAAAGTGGAC 
AGAGAACAGA AAGCTGCTGA GGAGGAAAAT ACTGTGGAGA AATCACTTAC AGAACTGCCA 
GGAGATTCAG CTTTAGTCAA CAATGTAACG CCATCTGTGA GAAGGGGTAG GCGAGCAAGA 
GGCAATGCAA ATGCTGTGAC TGAAACAGTC GTAACTGAAG CAAATTTGCC TGACTTGACA 
AACAATATCG TTTTACATAA AGAGCAAGAA AGCTCCAGTC AAGCATCTCC AGAAGCCAAA 
ACTCAAAGAG GAAAAAGGAA AAATCTTAAA AATACAAACG TTGATTCTGT AAGCAACTCT 
GCTGAAACAG ATGTTGCAAC ATTAGAATTA GAAGAGAGAC CTAACAAAAG ATCTACACGT 
GGTGTTAGAG GCACACAGCA AGTTGATGAG CTTAAAGAAC CAAAAGGAAA AAAAGCAAAA 
GTAGAACATG TGCCCAAACC TGCTGAACTA GTTCATATAC AGGAGAATCT AGCTAAAGAG 
AATGCATCTA GGGCGAAATC TGTGCAGTGG CACCCTCTTC TGACTGTTAA GGGGACACCT 
GAAAAGAATA CTGAAGAGAC TGTTAAGAAT CTTGCAGATG AAGAAGCTCC GGTCAGTGCA 
GTAAAATCTA GAGGAAGGAG GCGGCAAGAA GTAAAAGAAT CTGTTCCAGC AAAACGATCG 
CGTTCAAGAA GGGAGGAAAA TAATGCAGAG GCATTAACTT CTAGTAGTAC AACAGCTGAT 
GCAGTTCCTA TTGCTTTGGT TGCCAGTGAA AGTACTGAAA CTCCTAAGAA TAAGAGAGGA 
CGGAGGAAAG CAAACACACA ATTAGAGAAA GTTACTGATT CAGATCTTTC AGTGACAGAG 
GTTTCCTCTA AGCTAGACCT AGTGCCAGAG GTTGTAAACG AAAAGGCCGT AAACAAACGA 
AACACAGGTA AAAGAAACCT CAAGTCTTCA TCTGATAGTG ATTCTAGTAG TAAAGCTGTT 
GAACCAGTGC TGGAGACTAT TACCCAAAAG CGTGGACGAA GAGGAGCAGC AGCCAAAAGT 
CTAGATTCTG AGACTACTGA AGTTAAAACT GTTGAGTCTG CTGAAAATGT AACTTCAAAT 
AAAAGAAGGG GAAGAGCACC AAGTCAAAAA ACCAACAATC CTGAAACTAG CAAAACCACT 
ACTGAACCTG TTAATAAAAA TCAAGCAACT GAAGCTGTAG CCAAAAATAC AGATAATGCA 
GAAAGTCCTC CTAAATCAAG AAGAGGCTTA AAAAGAAAGG CAGCCACCAC TGATGTTCCG 
GAAGGCAACA CATCAGTAAG TGAAAATGGA CCCCGTGCCA ATGTAAGAGA AAGCACAAGA 
GGAGCTTCAA GGCAAAAAAC TGCAGCAAAA GAAAATCCAG TGGTTGTTGA AGAATCACCA 
GCAAAGAAAA AGAGAACAGA GCCATCTTCC AGTCCTGCTC AGAAAGGAGG AAAAACAAGA 
GCCACTGCAG CAAAACGGAA AAATGAGAAA CCAACAGAAG TTGTAAAGAC AAGGCAAACA 
TTGCGCACAC GGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905894.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761754.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.

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CloneID	OXa62328
Clone ID Related Accession (Same CDS sequence)	XM_031905894.1
Accession Version	XM_031905894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGG GGCATCCCCT GCCAGGAGGT CGCCCGCCAA GGCAGCTACC 
CCTGCCAGGA GGTCGCCCGC CAAGGCAGCT ACCCCTGCCA GGAGGTCGCC GGCCAAGGCA 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAAGAG GTCGCCGGCC 
AAGGCGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCGG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CGGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCGGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CACCAGCCAA GGCAGCTACC CCTGCCAAGA GGTCGCCAGT CAAGGCAGCT 
ACCCCTGCCA AGAGGTCTCC TGCCTCAGCA ACCCCTGCCA AGAGGTCTCC TGCTTCTACT 
TATACAAAAG GACGTTTCTC AATTTCACGA ATTGATACAC CACCGCAAAT AGATGTCCAT 
GCTCCTAACA CTGAGAAAAA TGAACTCTCT GCACAAACAC CCAGAAAATC ACGGAAGTCA 
ATATCATTAA AGAAAACGCC AGGAAGAAGA AGTAGAAAAC TTGAATCTTT TGAAATTTTG 
CGTTCCAGGC GAAGAAGTGG GGCAACTGAA GCTAACTTGC TTGTTGCAAA ATCATGGGCT 
GATGTTGTAA GAAGCGGTGT TCCAAAAAAT CAGAAGAAAT CTGAAAAGCA TGCTCGCAAA 
GCAGTACCTA CCAAGAGAAA GCCAAAGCCA AAGACGCCAG CACGAAGAAT TATTGACCGA 
ATTGGCACAG GACATGCTGA TTCTCCTGCC ACAATCCTTG TTGGAAGAGC TCATACAAGA 
ACTGTCGACC TAACTGGATA TGTTCCTAAA ATTGTTCAAA ACCAAGCAAT AAAAATCACC 
AATGACCAAA ATGAAAGCTT TACAGGTTTG AGTGAGTTGT TTAATACCCC TGCACATGTG 
AAACCAAGGA GAAGTGGTCG CTCAGGAGGC CTTCCGATGG AAACTCCGCA ATCTATAATT 
GTTGATATGT CACTGATGAA GACTCCAGAG GAATTAGGTG AAATGGTGGT TTCTCCAATT 
AATGGTAGCC CTGCTACAAC ACAGAAGAAA CAGTACAGTC GTGACGCAGT ATCTCGTCTT 
CTAAGTCCTG TGACGCCAGC ATCAGAAGAA CAGATATCCG AAGCTTCCAA AGACACTCAG 
TTGGTTGTAA AAGAGACAGC CACATTAAAT GAAACCCGTT TTATAAAAAA GGGTGCAAAG 
GCACAGATGA AAAAAAGAGG AACAATGGGT TTGACTGGTG TAAAAAGGAT TATGAGAACA 
CCAAAACAAA AAGGAAAGCA GGTTATTGAT CCTGTGGCGC TAAAGAAAAT GTTGAGAACA 
CCAAAACAAG TTGAATCACT TCCTGTAGAG TCCTCTGGCC CTAATGATCT AGTTGGACTG 
AAGGAAATCA TGAAAACTCC AAAAGTAAAA GGGCAACCAG TGGAGGATAT GACTGGCATA 
GCACGCATCA TGAAGACCCC AAGACGCAAG CAACAACCAG TAGAAGATAT GGCTGGTATT 
AAACGTATTA TGAAGACTCC TAAACAGAAA GGGACGCCTG TTGAGGATAT GACCGGAATC 
AAGCGTATTA TGACGACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGTATTA TGACAACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGCATTA TGAGAACCCC CAAGGAGAAA GGGCAACCTG TGGAGGATAT GGAAGGTTTA 
AGTCAGATTA TGAGAACACC AACTGAAAAT ACTCACCCAA TAGAAGAAAT ATTTGGGATG 
AAACAACTAA TAAAGACTCC TCCAAAGAGA GCACTTGGTG TTAGAGAGGG CACGTCTGGG 
ACACCATCAC AATCTGGCAA AAAGGCTACT CTGTTTCAGA CTAATCAAAA ATCGGCACAA 
GAAGAAGCAT TGCCAAAACT TACTTTGGAG AAAAATAATG AAAGCGAGGT TGCTGTACAA 
ACTGACAGAG AAACTGAAAA ATCTAAACCA ACCCCCAAAA GAGGTCGACC TGCCAAGGCT 
AGTGTGAAGC TTGCTTCAGA TGGTGGGAAG CCTGTTGATA ATGGCACATC TACACATGAA 
ACACACCCAG AAGAAAGCAG CAGAGAGGCT TCTCTCCAAG TACTGGCTAG TGATGATAAA 
TCCTCCACAG CTGCTGATAA AGCCACCCCA GCCAAACGAA GAGGTCGTCC ATCCAAGTCC 
AGTTTGAGTG GTTCACCAGG TGGTGGAAAT TCTGTTGAAA ACAACACATT CAGACATGAA 
AAAAATCCGG AAGAAGCCGC TCCTCTACAG GTACTCTCCA CTGAAAATAC TTCATCCTTG 
GGTGATGATA AAGCTACTCC AGCTAAAAGA GGTCGTCCAT GCAAACGTGG TATGAGTAAG 
TCACCAGCAT CTAGTAATAA AGATAAGTCT GTTGAAAAGA ATAGTACACC AGAGCATGAA 
AAGAACCTGG AAGAAAGCAA TGAAGGAACA TCTCTACAGG TACAGACCAT TGAAGATGCT 
TCTTCCACTA GTGCTGATAA AGCCACTCCG GCCAAAAGAA GAGGTCGTCC AGCTAAACGC 
AGCTTGAGTC ATTCACCAGG TTTAGAAAAA CCTGTTGTAG ATGTTCCAGA AGACCTAAGC 
ACCAGCAATG AGGTCTCTAA TATTCCTGAA GGATCAAGTC ATATCAATAC AGACCAAGAG 
AACAATGTTG ATTTACCAAT GGAAAAAGAG TCTGAACCGT TAAATCTAAA TATTGGAGCG 
AAAAAAGTCA CTAAGAAATC TCAGAGAAAG AAAGTCACAG AAGAGTCTGT TCAAATAGCA 
GTGGAAAACT TGGTTGTTGA ACAAGATGAT GAAAAATCTG CTCCCCAAAG ACGACGAGGA 
AGACCTCAAA GGGGTGGTCT TTCATCTACC ACTGAAGTTT TAGACGAAGC ATTACCAGAA 
GAGCCTCCAA CAAACAGACG AACTGGCAGG GGCCGAAAGA TTGCAGAAGT GGCAAAACCG 
CAACAAGTCA GTACAAGTGT TGAAGAATCT CAAGAAGTAA GAAACGAGGA ACTCTCAGCA 
ACTAAGACTC AGACTAAGTC TACCAGGCAT CAAAAAGCTG TATCTGAAGA CTCTACAGTT 
ACAGCAACTA AGAAGCCACG TGGAAGGCAA CCTAAAGTGG ACAGAGAACA GAAAGCTGCT 
GAGGAGGAAA ATACTGTGGA GAAATCACTT ACAGAACTGC CAGGAGATTC AGCTTTAGTC 
AACAATGTAA CGCCATCTGT GAGAAGGGGT AGGCGAGCAA GAGGCAATGC AAATGCTGTG 
ACTGAAACAG TCGTAACTGA AGCAAATTTG CCTGACTTGA CAAACAATAT CGTTTTACAT 
AAAGAGCAAG AAAGCTCCAG TCAAGCATCT CCAGAAGCCA AAACTCAAAG AGGAAAAAGG 
AAAAATCTTA AAAATACAAA CGTTGATTCT GTAAGCAACT CTGCTGAAAC AGATGTTGCA 
ACATTAGAAT TAGAAGAGAG ACCTAACAAA AGATCTACAC GTGGTGTTAG AGGCACACAG 
CAAGTTGATG AGCTTAAAGA ACCAAAAGGA AAAAAAGCAA AAGTAGAACA TGTGCCCAAA 
CCTGCTGAAC TAGTTCATAT ACAGGAGAAT CTAGCTAAAG AGAATGCATC TAGGGCGAAA 
TCTGTGCAGT GGCACCCTCT TCTGACTGTT AAGGGGACAC CTGAAAAGAA TACTGAAGAG 
ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT CCGGTCAGTG CAGTAAAATC TAGAGGAAGG 
AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA GCAAAACGAT CGCGTTCAAG AAGGGAGGAA 
AATAATGCAG AGGCATTAAC TTCTAGTAGT ACAACAGCTG ATGCAGTTCC TATTGCTTTG 
GTTGCCAGTG AAAGTACTGA AACTCCTAAG AATAAGAGAG GACGGAGGAA AGCAAACACA 
CAATTAGAGA AAGTTACTGA TTCAGATCTT TCAGTGACAG AGGTTTCCTC TAAGCTAGAC 
CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC GTAAACAAAC GAAACACAGG TAAAAGAAAC 
CTCAAGTCTT CATCTGATAG TGATTCTAGT AGTAAAGCTG TTGAACCAGT GCTGGAGACT 
ATTACCCAAA AGCGTGGACG AAGAGGAGCA GCAGCCAAAA GTCTAGATTC TGAGACTACT 
GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT GTAACTTCAA ATAAAAGAAG GGGAAGAGCA 
CCAAGTCAAA AAACCAACAA TCCTGAAACT AGCAAAACCA CTACTGAACC TGTTAATAAA 
AATCAAGCAA CTGAAGCTGT AGCCAAAAAT ACAGATAATG CAGAAAGTCC TCCTAAATCA 
AGAAGAGGCT TAAAAAGAAA GGCAGCCACC ACTGATGTTC CGGAAGGCAA CACATCAGTA 
AGTGAAAATG GACCCCGTGC CAATGTAAGA GAAAGCACAA GAGGAGCTTC AAGGCAAAAA 
ACTGCAGCAA AAGAAAATCC AGTGGTTGTT GAAGAATCAC CAGCAAAGAA AAAGAGAACA 
GAGCCATCTT CCAGTCCTGC TCAGAAAGGA GGAAAAACAA GAGCCACTGC AGCAAAACGG 
AAAAATGAGA AACCAACAGA AGTTGTAAAG ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761754.1
CDS	214..6813
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905894.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905894.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGG GGCATCCCCT GCCAGGAGGT CGCCCGCCAA GGCAGCTACC 
CCTGCCAGGA GGTCGCCCGC CAAGGCAGCT ACCCCTGCCA GGAGGTCGCC GGCCAAGGCA 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAAGAG GTCGCCGGCC 
AAGGCGGCAT CCCCTGCCAG GAGGTCGCCG GCCAAGGCGG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CGGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCGGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCT 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCGCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CACCAGCCAA GGCAGCTACC CCTGCCAAGA GGTCGCCAGT CAAGGCAGCT 
ACCCCTGCCA AGAGGTCTCC TGCCTCAGCA ACCCCTGCCA AGAGGTCTCC TGCTTCTACT 
TATACAAAAG GACGTTTCTC AATTTCACGA ATTGATACAC CACCGCAAAT AGATGTCCAT 
GCTCCTAACA CTGAGAAAAA TGAACTCTCT GCACAAACAC CCAGAAAATC ACGGAAGTCA 
ATATCATTAA AGAAAACGCC AGGAAGAAGA AGTAGAAAAC TTGAATCTTT TGAAATTTTG 
CGTTCCAGGC GAAGAAGTGG GGCAACTGAA GCTAACTTGC TTGTTGCAAA ATCATGGGCT 
GATGTTGTAA GAAGCGGTGT TCCAAAAAAT CAGAAGAAAT CTGAAAAGCA TGCTCGCAAA 
GCAGTACCTA CCAAGAGAAA GCCAAAGCCA AAGACGCCAG CACGAAGAAT TATTGACCGA 
ATTGGCACAG GACATGCTGA TTCTCCTGCC ACAATCCTTG TTGGAAGAGC TCATACAAGA 
ACTGTCGACC TAACTGGATA TGTTCCTAAA ATTGTTCAAA ACCAAGCAAT AAAAATCACC 
AATGACCAAA ATGAAAGCTT TACAGGTTTG AGTGAGTTGT TTAATACCCC TGCACATGTG 
AAACCAAGGA GAAGTGGTCG CTCAGGAGGC CTTCCGATGG AAACTCCGCA ATCTATAATT 
GTTGATATGT CACTGATGAA GACTCCAGAG GAATTAGGTG AAATGGTGGT TTCTCCAATT 
AATGGTAGCC CTGCTACAAC ACAGAAGAAA CAGTACAGTC GTGACGCAGT ATCTCGTCTT 
CTAAGTCCTG TGACGCCAGC ATCAGAAGAA CAGATATCCG AAGCTTCCAA AGACACTCAG 
TTGGTTGTAA AAGAGACAGC CACATTAAAT GAAACCCGTT TTATAAAAAA GGGTGCAAAG 
GCACAGATGA AAAAAAGAGG AACAATGGGT TTGACTGGTG TAAAAAGGAT TATGAGAACA 
CCAAAACAAA AAGGAAAGCA GGTTATTGAT CCTGTGGCGC TAAAGAAAAT GTTGAGAACA 
CCAAAACAAG TTGAATCACT TCCTGTAGAG TCCTCTGGCC CTAATGATCT AGTTGGACTG 
AAGGAAATCA TGAAAACTCC AAAAGTAAAA GGGCAACCAG TGGAGGATAT GACTGGCATA 
GCACGCATCA TGAAGACCCC AAGACGCAAG CAACAACCAG TAGAAGATAT GGCTGGTATT 
AAACGTATTA TGAAGACTCC TAAACAGAAA GGGACGCCTG TTGAGGATAT GACCGGAATC 
AAGCGTATTA TGACGACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGTATTA TGACAACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGCATTA TGAGAACCCC CAAGGAGAAA GGGCAACCTG TGGAGGATAT GGAAGGTTTA 
AGTCAGATTA TGAGAACACC AACTGAAAAT ACTCACCCAA TAGAAGAAAT ATTTGGGATG 
AAACAACTAA TAAAGACTCC TCCAAAGAGA GCACTTGGTG TTAGAGAGGG CACGTCTGGG 
ACACCATCAC AATCTGGCAA AAAGGCTACT CTGTTTCAGA CTAATCAAAA ATCGGCACAA 
GAAGAAGCAT TGCCAAAACT TACTTTGGAG AAAAATAATG AAAGCGAGGT TGCTGTACAA 
ACTGACAGAG AAACTGAAAA ATCTAAACCA ACCCCCAAAA GAGGTCGACC TGCCAAGGCT 
AGTGTGAAGC TTGCTTCAGA TGGTGGGAAG CCTGTTGATA ATGGCACATC TACACATGAA 
ACACACCCAG AAGAAAGCAG CAGAGAGGCT TCTCTCCAAG TACTGGCTAG TGATGATAAA 
TCCTCCACAG CTGCTGATAA AGCCACCCCA GCCAAACGAA GAGGTCGTCC ATCCAAGTCC 
AGTTTGAGTG GTTCACCAGG TGGTGGAAAT TCTGTTGAAA ACAACACATT CAGACATGAA 
AAAAATCCGG AAGAAGCCGC TCCTCTACAG GTACTCTCCA CTGAAAATAC TTCATCCTTG 
GGTGATGATA AAGCTACTCC AGCTAAAAGA GGTCGTCCAT GCAAACGTGG TATGAGTAAG 
TCACCAGCAT CTAGTAATAA AGATAAGTCT GTTGAAAAGA ATAGTACACC AGAGCATGAA 
AAGAACCTGG AAGAAAGCAA TGAAGGAACA TCTCTACAGG TACAGACCAT TGAAGATGCT 
TCTTCCACTA GTGCTGATAA AGCCACTCCG GCCAAAAGAA GAGGTCGTCC AGCTAAACGC 
AGCTTGAGTC ATTCACCAGG TTTAGAAAAA CCTGTTGTAG ATGTTCCAGA AGACCTAAGC 
ACCAGCAATG AGGTCTCTAA TATTCCTGAA GGATCAAGTC ATATCAATAC AGACCAAGAG 
AACAATGTTG ATTTACCAAT GGAAAAAGAG TCTGAACCGT TAAATCTAAA TATTGGAGCG 
AAAAAAGTCA CTAAGAAATC TCAGAGAAAG AAAGTCACAG AAGAGTCTGT TCAAATAGCA 
GTGGAAAACT TGGTTGTTGA ACAAGATGAT GAAAAATCTG CTCCCCAAAG ACGACGAGGA 
AGACCTCAAA GGGGTGGTCT TTCATCTACC ACTGAAGTTT TAGACGAAGC ATTACCAGAA 
GAGCCTCCAA CAAACAGACG AACTGGCAGG GGCCGAAAGA TTGCAGAAGT GGCAAAACCG 
CAACAAGTCA GTACAAGTGT TGAAGAATCT CAAGAAGTAA GAAACGAGGA ACTCTCAGCA 
ACTAAGACTC AGACTAAGTC TACCAGGCAT CAAAAAGCTG TATCTGAAGA CTCTACAGTT 
ACAGCAACTA AGAAGCCACG TGGAAGGCAA CCTAAAGTGG ACAGAGAACA GAAAGCTGCT 
GAGGAGGAAA ATACTGTGGA GAAATCACTT ACAGAACTGC CAGGAGATTC AGCTTTAGTC 
AACAATGTAA CGCCATCTGT GAGAAGGGGT AGGCGAGCAA GAGGCAATGC AAATGCTGTG 
ACTGAAACAG TCGTAACTGA AGCAAATTTG CCTGACTTGA CAAACAATAT CGTTTTACAT 
AAAGAGCAAG AAAGCTCCAG TCAAGCATCT CCAGAAGCCA AAACTCAAAG AGGAAAAAGG 
AAAAATCTTA AAAATACAAA CGTTGATTCT GTAAGCAACT CTGCTGAAAC AGATGTTGCA 
ACATTAGAAT TAGAAGAGAG ACCTAACAAA AGATCTACAC GTGGTGTTAG AGGCACACAG 
CAAGTTGATG AGCTTAAAGA ACCAAAAGGA AAAAAAGCAA AAGTAGAACA TGTGCCCAAA 
CCTGCTGAAC TAGTTCATAT ACAGGAGAAT CTAGCTAAAG AGAATGCATC TAGGGCGAAA 
TCTGTGCAGT GGCACCCTCT TCTGACTGTT AAGGGGACAC CTGAAAAGAA TACTGAAGAG 
ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT CCGGTCAGTG CAGTAAAATC TAGAGGAAGG 
AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA GCAAAACGAT CGCGTTCAAG AAGGGAGGAA 
AATAATGCAG AGGCATTAAC TTCTAGTAGT ACAACAGCTG ATGCAGTTCC TATTGCTTTG 
GTTGCCAGTG AAAGTACTGA AACTCCTAAG AATAAGAGAG GACGGAGGAA AGCAAACACA 
CAATTAGAGA AAGTTACTGA TTCAGATCTT TCAGTGACAG AGGTTTCCTC TAAGCTAGAC 
CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC GTAAACAAAC GAAACACAGG TAAAAGAAAC 
CTCAAGTCTT CATCTGATAG TGATTCTAGT AGTAAAGCTG TTGAACCAGT GCTGGAGACT 
ATTACCCAAA AGCGTGGACG AAGAGGAGCA GCAGCCAAAA GTCTAGATTC TGAGACTACT 
GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT GTAACTTCAA ATAAAAGAAG GGGAAGAGCA 
CCAAGTCAAA AAACCAACAA TCCTGAAACT AGCAAAACCA CTACTGAACC TGTTAATAAA 
AATCAAGCAA CTGAAGCTGT AGCCAAAAAT ACAGATAATG CAGAAAGTCC TCCTAAATCA 
AGAAGAGGCT TAAAAAGAAA GGCAGCCACC ACTGATGTTC CGGAAGGCAA CACATCAGTA 
AGTGAAAATG GACCCCGTGC CAATGTAAGA GAAAGCACAA GAGGAGCTTC AAGGCAAAAA 
ACTGCAGCAA AAGAAAATCC AGTGGTTGTT GAAGAATCAC CAGCAAAGAA AAAGAGAACA 
GAGCCATCTT CCAGTCCTGC TCAGAAAGGA GGAAAAACAA GAGCCACTGC AGCAAAACGG 
AAAAATGAGA AACCAACAGA AGTTGTAAAG ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905895.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761755.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X11, mRNA.

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CloneID	OXa62329
Clone ID Related Accession (Same CDS sequence)	XM_031905895.1
Accession Version	XM_031905895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCACC AGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTGAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGAT AAGTCTGTTG AAAAGAATAG TACACCAGAG 
CATGAAAAGA ACCTGGAAGA AAGCAATGAA GGAACATCTC TACAGGTACA GACCATTGAA 
GATGCTTCTT CCACTAGTGC TGATAAAGCC ACTCCGGCCA AAAGAAGAGG TCGTCCAGCT 
AAACGCAGCT TGAGTCATTC ACCAGGTTTA GAAAAACCTG TTGTAGATGT TCCAGAAGAC 
CTAAGCACCA GCAATGAGGT CTCTAATATT CCTGAAGGAT CAAGTCATAT CAATACAGAC 
CAAGAGAACA ATGTTGATTT ACCAATGGAA AAAGAGTCTG AACCGTTAAA TCTAAATATT 
GGAGCGAAAA AAGTCACTAA GAAATCTCAG AGAAAGAAAG TCACAGAAGA GTCTGTTCAA 
ATAGCAGTGG AAAACTTGGT TGTTGAACAA GATGATGAAA AATCTGCTCC CCAAAGACGA 
CGAGGAAGAC CTCAAAGGGG TGGTCTTTCA TCTACCACTG AAGTTTTAGA CGAAGCATTA 
CCAGAAGAGC CTCCAACAAA CAGACGAACT GGCAGGGGCC GAAAGATTGC AGAAGTGGCA 
AAACCGCAAC AAGTCAGTAC AAGTGTTGAA GAATCTCAAG AAGTAAGAAA CGAGGAACTC 
TCAGCAACTA AGACTCAGAC TAAGTCTACC AGGCATCAAA AAGCTGTATC TGAAGACTCT 
ACAGTTACAG CAACTAAGAA GCCACGTGGA AGGCAACCTA AAGTGGACAG AGAACAGAAA 
GCTGCTGAGG AGGAAAATAC TGTGGAGAAA TCACTTACAG AACTGCCAGG AGATTCAGCT 
TTAGTCAACA ATGTAACGCC ATCTGTGAGA AGGGGTAGGC GAGCAAGAGG CAATGCAAAT 
GCTGTGACTG AAACAGTCGT AACTGAAGCA AATTTGCCTG ACTTGACAAA CAATATCGTT 
TTACATAAAG AGCAAGAAAG CTCCAGTCAA GCATCTCCAG AAGCCAAAAC TCAAAGAGGA 
AAAAGGAAAA ATCTTAAAAA TACAAACGTT GATTCTGTAA GCAACTCTGC TGAAACAGAT 
GTTGCAACAT TAGAATTAGA AGAGAGACCT AACAAAAGAT CTACACGTGG TGTTAGAGGC 
ACACAGCAAG TTGATGAGCT TAAAGAACCA AAAGGAAAAA AAGCAAAAGT AGAACATGTG 
CCCAAACCTG CTGAACTAGT TCATATACAG GAGAATCTAG CTAAAGAGAA TGCATCTAGG 
GCGAAATCTG TGCAGTGGCA CCCTCTTCTG ACTGTTAAGG GGACACCTGA AAAGAATACT 
GAAGAGACTG TTAAGAATCT TGCAGATGAA GAAGCTCCGG TCAGTGCAGT AAAATCTAGA 
GGAAGGAGGC GGCAAGAAGT AAAAGAATCT GTTCCAGCAA AACGATCGCG TTCAAGAAGG 
GAGGAAAATA ATGCAGAGGC ATTAACTTCT AGTAGTACAA CAGCTGATGC AGTTCCTATT 
GCTTTGGTTG CCAGTGAAAG TACTGAAACT CCTAAGAATA AGAGAGGACG GAGGAAAGCA 
AACACACAAT TAGAGAAAGT TACTGATTCA GATCTTTCAG TGACAGAGGT TTCCTCTAAG 
CTAGACCTAG TGCCAGAGGT TGTAAACGAA AAGGCCGTAA ACAAACGAAA CACAGGTAAA 
AGAAACCTCA AGTCTTCATC TGATAGTGAT TCTAGTAGTA AAGCTGTTGA ACCAGTGCTG 
GAGACTATTA CCCAAAAGCG TGGACGAAGA GGAGCAGCAG CCAAAAGTCT AGATTCTGAG 
ACTACTGAAG TTAAAACTGT TGAGTCTGCT GAAAATGTAA CTTCAAATAA AAGAAGGGGA 
AGAGCACCAA GTCAAAAAAC CAACAATCCT GAAACTAGCA AAACCACTAC TGAACCTGTT 
AATAAAAATC AAGCAACTGA AGCTGTAGCC AAAAATACAG ATAATGCAGA AAGTCCTCCT 
AAATCAAGAA GAGGCTTAAA AAGAAAGGCA GCCACCACTG ATGTTCCGGA AGGCAACACA 
TCAGTAAGTG AAAATGGACC CCGTGCCAAT GTAAGAGAAA GCACAAGAGG AGCTTCAAGG 
CAAAAAACTG CAGCAAAAGA AAATCCAGTG GTTGTTGAAG AATCACCAGC AAAGAAAAAG 
AGAACAGAGC CATCTTCCAG TCCTGCTCAG AAAGGAGGAA AAACAAGAGC CACTGCAGCA 
AAACGGAAAA ATGAGAAACC AACAGAAGTT GTAAAGACAA GGCAAACATT GCGCACACGG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761755.1
CDS	213..6698
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905895.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905895.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCAA AGGGGGCATC CCCTGCCAAG 
AGGTCGCCGG CCAAGGCGGC ATCCCCTGCC AGGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGGCGGCT 
ACCCCGGCCA AGAGGTCACC AGCCAAGGCA GCTACCCCTG CCAAGAGGTC GCCAGTCAAG 
GCAGCTACCC CTGCCAAGAG GTCTCCTGCC TCAGCAACCC CTGCCAAGAG GTCTCCTGCT 
TCTACTTATA CAAAAGGACG TTTCTCAATT TCACGAATTG ATACACCACC GCAAATAGAT 
GTCCATGCTC CTAACACTGA GAAAAATGAA CTCTCTGCAC AAACACCCAG AAAATCACGG 
AAGTCAATAT CATTAAAGAA AACGCCAGGA AGAAGAAGTA GAAAACTTGA ATCTTTTGAA 
ATTTTGCGTT CCAGGCGAAG AAGTGGGGCA ACTGAAGCTA ACTTGCTTGT TGCAAAATCA 
TGGGCTGATG TTGTAAGAAG CGGTGTTCCA AAAAATCAGA AGAAATCTGA AAAGCATGCT 
CGCAAAGCAG TACCTACCAA GAGAAAGCCA AAGCCAAAGA CGCCAGCACG AAGAATTATT 
GACCGAATTG GCACAGGACA TGCTGATTCT CCTGCCACAA TCCTTGTTGG AAGAGCTCAT 
ACAAGAACTG TCGACCTAAC TGGATATGTT CCTAAAATTG TTCAAAACCA AGCAATAAAA 
ATCACCAATG ACCAAAATGA AAGCTTTACA GGTTTGAGTG AGTTGTTTAA TACCCCTGCA 
CATGTGAAAC CAAGGAGAAG TGGTCGCTCA GGAGGCCTTC CGATGGAAAC TCCGCAATCT 
ATAATTGTTG ATATGTCACT GATGAAGACT CCAGAGGAAT TAGGTGAAAT GGTGGTTTCT 
CCAATTAATG GTAGCCCTGC TACAACACAG AAGAAACAGT ACAGTCGTGA CGCAGTATCT 
CGTCTTCTAA GTCCTGTGAC GCCAGCATCA GAAGAACAGA TATCCGAAGC TTCCAAAGAC 
ACTCAGTTGG TTGTAAAAGA GACAGCCACA TTAAATGAAA CCCGTTTTAT AAAAAAGGGT 
GCAAAGGCAC AGATGAAAAA AAGAGGAACA ATGGGTTTGA CTGGTGTAAA AAGGATTATG 
AGAACACCAA AACAAAAAGG AAAGCAGGTT ATTGATCCTG TGGCGCTAAA GAAAATGTTG 
AGAACACCAA AACAAGTTGA ATCACTTCCT GTAGAGTCCT CTGGCCCTAA TGATCTAGTT 
GGACTGAAGG AAATCATGAA AACTCCAAAA GTAAAAGGGC AACCAGTGGA GGATATGACT 
GGCATAGCAC GCATCATGAA GACCCCAAGA CGCAAGCAAC AACCAGTAGA AGATATGGCT 
GGTATTAAAC GTATTATGAA GACTCCTAAA CAGAAAGGGA CGCCTGTTGA GGATATGACC 
GGAATCAAGC GTATTATGAC GACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GTATTATGAC AACCCCCAAG GAGAAAGGGC AACCCGTGGA GGACATGGTG 
GGAATCAAAC GCATTATGAG AACCCCCAAG GAGAAAGGGC AACCTGTGGA GGATATGGAA 
GGTTTAAGTC AGATTATGAG AACACCAACT GAAAATACTC ACCCAATAGA AGAAATATTT 
GGGATGAAAC AACTAATAAA GACTCCTCCA AAGAGAGCAC TTGGTGTTAG AGAGGGCACG 
TCTGGGACAC CATCACAATC TGGCAAAAAG GCTACTCTGT TTCAGACTAA TCAAAAATCG 
GCACAAGAAG AAGCATTGCC AAAACTTACT TTGGAGAAAA ATAATGAAAG CGAGGTTGCT 
GTACAAACTG ACAGAGAAAC TGAAAAATCT AAACCAACCC CCAAAAGAGG TCGACCTGCC 
AAGGCTAGTG TGAAGCTTGC TTCAGATGGT GGGAAGCCTG TTGATAATGG CACATCTACA 
CATGAAACAC ACCCAGAAGA AAGCAGCAGA GAGGCTTCTC TCCAAGTACT GGCTAGTGAT 
GATAAATCCT CCACAGCTGC TGATAAAGCC ACCCCAGCCA AACGAAGAGG TCGTCCATCC 
AAGTCCAGTT TGAGTGGTTC ACCAGGTGGT GGAAATTCTG TTGAAAACAA CACATTCAGA 
CATGAAAAAA ATCCGGAAGA AGCCGCTCCT CTACAGGTAC TCTCCACTGA AAATACTTCA 
TCCTTGGGTG ATGATAAAGC TACTCCAGCT AAAAGAGGTC GTCCATGCAA ACGTGGTATG 
AGTAAGTCAC CAGCATCTAG TAATAAAGAT AAGTCTGTTG AAAAGAATAG TACACCAGAG 
CATGAAAAGA ACCTGGAAGA AAGCAATGAA GGAACATCTC TACAGGTACA GACCATTGAA 
GATGCTTCTT CCACTAGTGC TGATAAAGCC ACTCCGGCCA AAAGAAGAGG TCGTCCAGCT 
AAACGCAGCT TGAGTCATTC ACCAGGTTTA GAAAAACCTG TTGTAGATGT TCCAGAAGAC 
CTAAGCACCA GCAATGAGGT CTCTAATATT CCTGAAGGAT CAAGTCATAT CAATACAGAC 
CAAGAGAACA ATGTTGATTT ACCAATGGAA AAAGAGTCTG AACCGTTAAA TCTAAATATT 
GGAGCGAAAA AAGTCACTAA GAAATCTCAG AGAAAGAAAG TCACAGAAGA GTCTGTTCAA 
ATAGCAGTGG AAAACTTGGT TGTTGAACAA GATGATGAAA AATCTGCTCC CCAAAGACGA 
CGAGGAAGAC CTCAAAGGGG TGGTCTTTCA TCTACCACTG AAGTTTTAGA CGAAGCATTA 
CCAGAAGAGC CTCCAACAAA CAGACGAACT GGCAGGGGCC GAAAGATTGC AGAAGTGGCA 
AAACCGCAAC AAGTCAGTAC AAGTGTTGAA GAATCTCAAG AAGTAAGAAA CGAGGAACTC 
TCAGCAACTA AGACTCAGAC TAAGTCTACC AGGCATCAAA AAGCTGTATC TGAAGACTCT 
ACAGTTACAG CAACTAAGAA GCCACGTGGA AGGCAACCTA AAGTGGACAG AGAACAGAAA 
GCTGCTGAGG AGGAAAATAC TGTGGAGAAA TCACTTACAG AACTGCCAGG AGATTCAGCT 
TTAGTCAACA ATGTAACGCC ATCTGTGAGA AGGGGTAGGC GAGCAAGAGG CAATGCAAAT 
GCTGTGACTG AAACAGTCGT AACTGAAGCA AATTTGCCTG ACTTGACAAA CAATATCGTT 
TTACATAAAG AGCAAGAAAG CTCCAGTCAA GCATCTCCAG AAGCCAAAAC TCAAAGAGGA 
AAAAGGAAAA ATCTTAAAAA TACAAACGTT GATTCTGTAA GCAACTCTGC TGAAACAGAT 
GTTGCAACAT TAGAATTAGA AGAGAGACCT AACAAAAGAT CTACACGTGG TGTTAGAGGC 
ACACAGCAAG TTGATGAGCT TAAAGAACCA AAAGGAAAAA AAGCAAAAGT AGAACATGTG 
CCCAAACCTG CTGAACTAGT TCATATACAG GAGAATCTAG CTAAAGAGAA TGCATCTAGG 
GCGAAATCTG TGCAGTGGCA CCCTCTTCTG ACTGTTAAGG GGACACCTGA AAAGAATACT 
GAAGAGACTG TTAAGAATCT TGCAGATGAA GAAGCTCCGG TCAGTGCAGT AAAATCTAGA 
GGAAGGAGGC GGCAAGAAGT AAAAGAATCT GTTCCAGCAA AACGATCGCG TTCAAGAAGG 
GAGGAAAATA ATGCAGAGGC ATTAACTTCT AGTAGTACAA CAGCTGATGC AGTTCCTATT 
GCTTTGGTTG CCAGTGAAAG TACTGAAACT CCTAAGAATA AGAGAGGACG GAGGAAAGCA 
AACACACAAT TAGAGAAAGT TACTGATTCA GATCTTTCAG TGACAGAGGT TTCCTCTAAG 
CTAGACCTAG TGCCAGAGGT TGTAAACGAA AAGGCCGTAA ACAAACGAAA CACAGGTAAA 
AGAAACCTCA AGTCTTCATC TGATAGTGAT TCTAGTAGTA AAGCTGTTGA ACCAGTGCTG 
GAGACTATTA CCCAAAAGCG TGGACGAAGA GGAGCAGCAG CCAAAAGTCT AGATTCTGAG 
ACTACTGAAG TTAAAACTGT TGAGTCTGCT GAAAATGTAA CTTCAAATAA AAGAAGGGGA 
AGAGCACCAA GTCAAAAAAC CAACAATCCT GAAACTAGCA AAACCACTAC TGAACCTGTT 
AATAAAAATC AAGCAACTGA AGCTGTAGCC AAAAATACAG ATAATGCAGA AAGTCCTCCT 
AAATCAAGAA GAGGCTTAAA AAGAAAGGCA GCCACCACTG ATGTTCCGGA AGGCAACACA 
TCAGTAAGTG AAAATGGACC CCGTGCCAAT GTAAGAGAAA GCACAAGAGG AGCTTCAAGG 
CAAAAAACTG CAGCAAAAGA AAATCCAGTG GTTGTTGAAG AATCACCAGC AAAGAAAAAG 
AGAACAGAGC CATCTTCCAG TCCTGCTCAG AAAGGAGGAA AAACAAGAGC CACTGCAGCA 
AAACGGAAAA ATGAGAAACC AACAGAAGTT GTAAAGACAA GGCAAACATT GCGCACACGG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905897.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761757.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X12, mRNA.

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CloneID	OXa62330
Clone ID Related Accession (Same CDS sequence)	XM_031905897.1
Accession Version	XM_031905897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CACCAGCCAA GGCAGCTACC CCTGCCAAGA GGTCGCCAGT CAAGGCAGCT 
ACCCCTGCCA AGAGGTCTCC TGCCTCAGCA ACCCCTGCCA AGAGGTCTCC TGCTTCTACT 
TATACAAAAG GACGTTTCTC AATTTCACGA ATTGATACAC CACCGCAAAT AGATGTCCAT 
GCTCCTAACA CTGAGAAAAA TGAACTCTCT GCACAAACAC CCAGAAAATC ACGGAAGTCA 
ATATCATTAA AGAAAACGCC AGGAAGAAGA AGTAGAAAAC TTGAATCTTT TGAAATTTTG 
CGTTCCAGGC GAAGAAGTGG GGCAACTGAA GCTAACTTGC TTGTTGCAAA ATCATGGGCT 
GATGTTGTAA GAAGCGGTGT TCCAAAAAAT CAGAAGAAAT CTGAAAAGCA TGCTCGCAAA 
GCAGTACCTA CCAAGAGAAA GCCAAAGCCA AAGACGCCAG CACGAAGAAT TATTGACCGA 
ATTGGCACAG GACATGCTGA TTCTCCTGCC ACAATCCTTG TTGGAAGAGC TCATACAAGA 
ACTGTCGACC TAACTGGATA TGTTCCTAAA ATTGTTCAAA ACCAAGCAAT AAAAATCACC 
AATGACCAAA ATGAAAGCTT TACAGGTTTG AGTGAGTTGT TTAATACCCC TGCACATGTG 
AAACCAAGGA GAAGTGGTCG CTCAGGAGGC CTTCCGATGG AAACTCCGCA ATCTATAATT 
GTTGATATGT CACTGATGAA GACTCCAGAG GAATTAGGTG AAATGGTGGT TTCTCCAATT 
AATGGTAGCC CTGCTACAAC ACAGAAGAAA CAGTACAGTC GTGACGCAGT ATCTCGTCTT 
CTAAGTCCTG TGACGCCAGC ATCAGAAGAA CAGATATCCG AAGCTTCCAA AGACACTCAG 
TTGGTTGTAA AAGAGACAGC CACATTAAAT GAAACCCGTT TTATAAAAAA GGGTGCAAAG 
GCACAGATGA AAAAAAGAGG AACAATGGGT TTGACTGGTG TAAAAAGGAT TATGAGAACA 
CCAAAACAAA AAGGAAAGCA GGTTATTGAT CCTGTGGCGC TAAAGAAAAT GTTGAGAACA 
CCAAAACAAG TTGAATCACT TCCTGTAGAG TCCTCTGGCC CTAATGATCT AGTTGGACTG 
AAGGAAATCA TGAAAACTCC AAAAGTAAAA GGGCAACCAG TGGAGGATAT GACTGGCATA 
GCACGCATCA TGAAGACCCC AAGACGCAAG CAACAACCAG TAGAAGATAT GGCTGGTATT 
AAACGTATTA TGAAGACTCC TAAACAGAAA GGGACGCCTG TTGAGGATAT GACCGGAATC 
AAGCGTATTA TGACGACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGTATTA TGACAACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGCATTA TGAGAACCCC CAAGGAGAAA GGGCAACCTG TGGAGGATAT GGAAGGTTTA 
AGTCAGATTA TGAGAACACC AACTGAAAAT ACTCACCCAA TAGAAGAAAT ATTTGGGATG 
AAACAACTAA TAAAGACTCC TCCAAAGAGA GCACTTGGTG TTAGAGAGGG CACGTCTGGG 
ACACCATCAC AATCTGGCAA AAAGGCTACT CTGTTTCAGA CTAATCAAAA ATCGGCACAA 
GAAGAAGCAT TGCCAAAACT TACTTTGGAG AAAAATAATG AAAGCGAGGT TGCTGTACAA 
ACTGACAGAG AAACTGAAAA ATCTAAACCA ACCCCCAAAA GAGGTCGACC TGCCAAGGCT 
AGTGTGAAGC TTGCTTCAGA TGGTGGGAAG CCTGTTGATA ATGGCACATC TACACATGAA 
ACACACCCAG AAGAAAGCAG CAGAGAGGCT TCTCTCCAAG TACTGGCTAG TGATGATAAA 
TCCTCCACAG CTGCTGATAA AGCCACCCCA GCCAAACGAA GAGGTCGTCC ATCCAAGTCC 
AGTTTGAGTG GTTCACCAGG TGGTGGAAAT TCTGTTGAAA ACAACACATT CAGACATGAA 
AAAAATCCGG AAGAAGCCGC TCCTCTACAG GTACTCTCCA CTGAAAATAC TTCATCCTTG 
GGTGATGATA AAGCTACTCC AGCTAAAAGA GGTCGTCCAT GCAAACGTGG TATGAGTAAG 
TCACCAGCAT CTAGTAATAA AGATAAGTCT GTTGAAAAGA ATAGTACACC AGAGCATGAA 
AAGAACCTGG AAGAAAGCAA TGAAGGAACA TCTCTACAGG TACAGACCAT TGAAGATGCT 
TCTTCCACTA GTGCTGATAA AGCCACTCCG GCCAAAAGAA GAGGTCGTCC AGCTAAACGC 
AGCTTGAGTC ATTCACCAGG TTTAGAAAAA CCTGTTGTAG ATGTTCCAGA AGACCTAAGC 
ACCAGCAATG AGGTCTCTAA TATTCCTGAA GGATCAAGTC ATATCAATAC AGACCAAGAG 
AACAATGTTG ATTTACCAAT GGAAAAAGAG TCTGAACCGT TAAATCTAAA TATTGGAGCG 
AAAAAAGTCA CTAAGAAATC TCAGAGAAAG AAAGTCACAG AAGAGTCTGT TCAAATAGCA 
GTGGAAAACT TGGTTGTTGA ACAAGATGAT GAAAAATCTG CTCCCCAAAG ACGACGAGGA 
AGACCTCAAA GGGGTGGTCT TTCATCTACC ACTGAAGTTT TAGACGAAGC ATTACCAGAA 
GAGCCTCCAA CAAACAGACG AACTGGCAGG GGCCGAAAGA TTGCAGAAGT GGCAAAACCG 
CAACAAGTCA GTACAAGTGT TGAAGAATCT CAAGAAGTAA GAAACGAGGA ACTCTCAGCA 
ACTAAGACTC AGACTAAGTC TACCAGGCAT CAAAAAGCTG TATCTGAAGA CTCTACAGTT 
ACAGCAACTA AGAAGCCACG TGGAAGGCAA CCTAAAGTGG ACAGAGAACA GAAAGCTGCT 
GAGGAGGAAA ATACTGTGGA GAAATCACTT ACAGAACTGC CAGGAGATTC AGCTTTAGTC 
AACAATGTAA CGCCATCTGT GAGAAGGGGT AGGCGAGCAA GAGGCAATGC AAATGCTGTG 
ACTGAAACAG TCGTAACTGA AGCAAATTTG CCTGACTTGA CAAACAATAT CGTTTTACAT 
AAAGAGCAAG AAAGCTCCAG TCAAGCATCT CCAGAAGCCA AAACTCAAAG AGGAAAAAGG 
AAAAATCTTA AAAATACAAA CGTTGATTCT GTAAGCAACT CTGCTGAAAC AGATGTTGCA 
ACATTAGAAT TAGAAGAGAG ACCTAACAAA AGATCTACAC GTGGTGTTAG AGGCACACAG 
CAAGTTGATG AGCTTAAAGA ACCAAAAGGA AAAAAAGCAA AAGTAGAACA TGTGCCCAAA 
CCTGCTGAAC TAGTTCATAT ACAGGAGAAT CTAGCTAAAG AGAATGCATC TAGGGCGAAA 
TCTGTGCAGT GGCACCCTCT TCTGACTGTT AAGGGGACAC CTGAAAAGAA TACTGAAGAG 
ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT CCGGTCAGTG CAGTAAAATC TAGAGGAAGG 
AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA GCAAAACGAT CGCGTTCAAG AAGGGAGGAA 
AATAATGCAG AGGCATTAAC TTCTAGTAGT ACAACAGCTG ATGCAGTTCC TATTGCTTTG 
GTTGCCAGTG AAAGTACTGA AACTCCTAAG AATAAGAGAG GACGGAGGAA AGCAAACACA 
CAATTAGAGA AAGTTACTGA TTCAGATCTT TCAGTGACAG AGGTTTCCTC TAAGCTAGAC 
CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC GTAAACAAAC GAAACACAGG TAAAAGAAAC 
CTCAAGTCTT CATCTGATAG TGATTCTAGT AGTAAAGCTG TTGAACCAGT GCTGGAGACT 
ATTACCCAAA AGCGTGGACG AAGAGGAGCA GCAGCCAAAA GTCTAGATTC TGAGACTACT 
GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT GTAACTTCAA ATAAAAGAAG GGGAAGAGCA 
CCAAGTCAAA AAACCAACAA TCCTGAAACT AGCAAAACCA CTACTGAACC TGTTAATAAA 
AATCAAGCAA CTGAAGCTGT AGCCAAAAAT ACAGATAATG CAGAAAGTCC TCCTAAATCA 
AGAAGAGGCT TAAAAAGAAA GGCAGCCACC ACTGATGTTC CGGAAGGCAA CACATCAGTA 
AGTGAAAATG GACCCCGTGC CAATGTAAGA GAAAGCACAA GAGGAGCTTC AAGGCAAAAA 
ACTGCAGCAA AAGAAAATCC AGTGGTTGTT GAAGAATCAC CAGCAAAGAA AAAGAGAACA 
GAGCCATCTT CCAGTCCTGC TCAGAAAGGA GGAAAAACAA GAGCCACTGC AGCAAAACGG 
AAAAATGAGA AACCAACAGA AGTTGTAAAG ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761757.1
CDS	212..6631
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905897.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905897.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCAAAG GGGGCATCCC CTGCCAGGAG GTCGCCCGCC 
AAGGCAGCTA CCCCTGCCAG GAGGTCGCCC GCCAAGGCAG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CAGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CACCAGCCAA GGCAGCTACC CCTGCCAAGA GGTCGCCAGT CAAGGCAGCT 
ACCCCTGCCA AGAGGTCTCC TGCCTCAGCA ACCCCTGCCA AGAGGTCTCC TGCTTCTACT 
TATACAAAAG GACGTTTCTC AATTTCACGA ATTGATACAC CACCGCAAAT AGATGTCCAT 
GCTCCTAACA CTGAGAAAAA TGAACTCTCT GCACAAACAC CCAGAAAATC ACGGAAGTCA 
ATATCATTAA AGAAAACGCC AGGAAGAAGA AGTAGAAAAC TTGAATCTTT TGAAATTTTG 
CGTTCCAGGC GAAGAAGTGG GGCAACTGAA GCTAACTTGC TTGTTGCAAA ATCATGGGCT 
GATGTTGTAA GAAGCGGTGT TCCAAAAAAT CAGAAGAAAT CTGAAAAGCA TGCTCGCAAA 
GCAGTACCTA CCAAGAGAAA GCCAAAGCCA AAGACGCCAG CACGAAGAAT TATTGACCGA 
ATTGGCACAG GACATGCTGA TTCTCCTGCC ACAATCCTTG TTGGAAGAGC TCATACAAGA 
ACTGTCGACC TAACTGGATA TGTTCCTAAA ATTGTTCAAA ACCAAGCAAT AAAAATCACC 
AATGACCAAA ATGAAAGCTT TACAGGTTTG AGTGAGTTGT TTAATACCCC TGCACATGTG 
AAACCAAGGA GAAGTGGTCG CTCAGGAGGC CTTCCGATGG AAACTCCGCA ATCTATAATT 
GTTGATATGT CACTGATGAA GACTCCAGAG GAATTAGGTG AAATGGTGGT TTCTCCAATT 
AATGGTAGCC CTGCTACAAC ACAGAAGAAA CAGTACAGTC GTGACGCAGT ATCTCGTCTT 
CTAAGTCCTG TGACGCCAGC ATCAGAAGAA CAGATATCCG AAGCTTCCAA AGACACTCAG 
TTGGTTGTAA AAGAGACAGC CACATTAAAT GAAACCCGTT TTATAAAAAA GGGTGCAAAG 
GCACAGATGA AAAAAAGAGG AACAATGGGT TTGACTGGTG TAAAAAGGAT TATGAGAACA 
CCAAAACAAA AAGGAAAGCA GGTTATTGAT CCTGTGGCGC TAAAGAAAAT GTTGAGAACA 
CCAAAACAAG TTGAATCACT TCCTGTAGAG TCCTCTGGCC CTAATGATCT AGTTGGACTG 
AAGGAAATCA TGAAAACTCC AAAAGTAAAA GGGCAACCAG TGGAGGATAT GACTGGCATA 
GCACGCATCA TGAAGACCCC AAGACGCAAG CAACAACCAG TAGAAGATAT GGCTGGTATT 
AAACGTATTA TGAAGACTCC TAAACAGAAA GGGACGCCTG TTGAGGATAT GACCGGAATC 
AAGCGTATTA TGACGACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGTATTA TGACAACCCC CAAGGAGAAA GGGCAACCCG TGGAGGACAT GGTGGGAATC 
AAACGCATTA TGAGAACCCC CAAGGAGAAA GGGCAACCTG TGGAGGATAT GGAAGGTTTA 
AGTCAGATTA TGAGAACACC AACTGAAAAT ACTCACCCAA TAGAAGAAAT ATTTGGGATG 
AAACAACTAA TAAAGACTCC TCCAAAGAGA GCACTTGGTG TTAGAGAGGG CACGTCTGGG 
ACACCATCAC AATCTGGCAA AAAGGCTACT CTGTTTCAGA CTAATCAAAA ATCGGCACAA 
GAAGAAGCAT TGCCAAAACT TACTTTGGAG AAAAATAATG AAAGCGAGGT TGCTGTACAA 
ACTGACAGAG AAACTGAAAA ATCTAAACCA ACCCCCAAAA GAGGTCGACC TGCCAAGGCT 
AGTGTGAAGC TTGCTTCAGA TGGTGGGAAG CCTGTTGATA ATGGCACATC TACACATGAA 
ACACACCCAG AAGAAAGCAG CAGAGAGGCT TCTCTCCAAG TACTGGCTAG TGATGATAAA 
TCCTCCACAG CTGCTGATAA AGCCACCCCA GCCAAACGAA GAGGTCGTCC ATCCAAGTCC 
AGTTTGAGTG GTTCACCAGG TGGTGGAAAT TCTGTTGAAA ACAACACATT CAGACATGAA 
AAAAATCCGG AAGAAGCCGC TCCTCTACAG GTACTCTCCA CTGAAAATAC TTCATCCTTG 
GGTGATGATA AAGCTACTCC AGCTAAAAGA GGTCGTCCAT GCAAACGTGG TATGAGTAAG 
TCACCAGCAT CTAGTAATAA AGATAAGTCT GTTGAAAAGA ATAGTACACC AGAGCATGAA 
AAGAACCTGG AAGAAAGCAA TGAAGGAACA TCTCTACAGG TACAGACCAT TGAAGATGCT 
TCTTCCACTA GTGCTGATAA AGCCACTCCG GCCAAAAGAA GAGGTCGTCC AGCTAAACGC 
AGCTTGAGTC ATTCACCAGG TTTAGAAAAA CCTGTTGTAG ATGTTCCAGA AGACCTAAGC 
ACCAGCAATG AGGTCTCTAA TATTCCTGAA GGATCAAGTC ATATCAATAC AGACCAAGAG 
AACAATGTTG ATTTACCAAT GGAAAAAGAG TCTGAACCGT TAAATCTAAA TATTGGAGCG 
AAAAAAGTCA CTAAGAAATC TCAGAGAAAG AAAGTCACAG AAGAGTCTGT TCAAATAGCA 
GTGGAAAACT TGGTTGTTGA ACAAGATGAT GAAAAATCTG CTCCCCAAAG ACGACGAGGA 
AGACCTCAAA GGGGTGGTCT TTCATCTACC ACTGAAGTTT TAGACGAAGC ATTACCAGAA 
GAGCCTCCAA CAAACAGACG AACTGGCAGG GGCCGAAAGA TTGCAGAAGT GGCAAAACCG 
CAACAAGTCA GTACAAGTGT TGAAGAATCT CAAGAAGTAA GAAACGAGGA ACTCTCAGCA 
ACTAAGACTC AGACTAAGTC TACCAGGCAT CAAAAAGCTG TATCTGAAGA CTCTACAGTT 
ACAGCAACTA AGAAGCCACG TGGAAGGCAA CCTAAAGTGG ACAGAGAACA GAAAGCTGCT 
GAGGAGGAAA ATACTGTGGA GAAATCACTT ACAGAACTGC CAGGAGATTC AGCTTTAGTC 
AACAATGTAA CGCCATCTGT GAGAAGGGGT AGGCGAGCAA GAGGCAATGC AAATGCTGTG 
ACTGAAACAG TCGTAACTGA AGCAAATTTG CCTGACTTGA CAAACAATAT CGTTTTACAT 
AAAGAGCAAG AAAGCTCCAG TCAAGCATCT CCAGAAGCCA AAACTCAAAG AGGAAAAAGG 
AAAAATCTTA AAAATACAAA CGTTGATTCT GTAAGCAACT CTGCTGAAAC AGATGTTGCA 
ACATTAGAAT TAGAAGAGAG ACCTAACAAA AGATCTACAC GTGGTGTTAG AGGCACACAG 
CAAGTTGATG AGCTTAAAGA ACCAAAAGGA AAAAAAGCAA AAGTAGAACA TGTGCCCAAA 
CCTGCTGAAC TAGTTCATAT ACAGGAGAAT CTAGCTAAAG AGAATGCATC TAGGGCGAAA 
TCTGTGCAGT GGCACCCTCT TCTGACTGTT AAGGGGACAC CTGAAAAGAA TACTGAAGAG 
ACTGTTAAGA ATCTTGCAGA TGAAGAAGCT CCGGTCAGTG CAGTAAAATC TAGAGGAAGG 
AGGCGGCAAG AAGTAAAAGA ATCTGTTCCA GCAAAACGAT CGCGTTCAAG AAGGGAGGAA 
AATAATGCAG AGGCATTAAC TTCTAGTAGT ACAACAGCTG ATGCAGTTCC TATTGCTTTG 
GTTGCCAGTG AAAGTACTGA AACTCCTAAG AATAAGAGAG GACGGAGGAA AGCAAACACA 
CAATTAGAGA AAGTTACTGA TTCAGATCTT TCAGTGACAG AGGTTTCCTC TAAGCTAGAC 
CTAGTGCCAG AGGTTGTAAA CGAAAAGGCC GTAAACAAAC GAAACACAGG TAAAAGAAAC 
CTCAAGTCTT CATCTGATAG TGATTCTAGT AGTAAAGCTG TTGAACCAGT GCTGGAGACT 
ATTACCCAAA AGCGTGGACG AAGAGGAGCA GCAGCCAAAA GTCTAGATTC TGAGACTACT 
GAAGTTAAAA CTGTTGAGTC TGCTGAAAAT GTAACTTCAA ATAAAAGAAG GGGAAGAGCA 
CCAAGTCAAA AAACCAACAA TCCTGAAACT AGCAAAACCA CTACTGAACC TGTTAATAAA 
AATCAAGCAA CTGAAGCTGT AGCCAAAAAT ACAGATAATG CAGAAAGTCC TCCTAAATCA 
AGAAGAGGCT TAAAAAGAAA GGCAGCCACC ACTGATGTTC CGGAAGGCAA CACATCAGTA 
AGTGAAAATG GACCCCGTGC CAATGTAAGA GAAAGCACAA GAGGAGCTTC AAGGCAAAAA 
ACTGCAGCAA AAGAAAATCC AGTGGTTGTT GAAGAATCAC CAGCAAAGAA AAAGAGAACA 
GAGCCATCTT CCAGTCCTGC TCAGAAAGGA GGAAAAACAA GAGCCACTGC AGCAAAACGG 
AAAAATGAGA AACCAACAGA AGTTGTAAAG ACAAGGCAAA CATTGCGCAC ACGGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905898.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761758.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X13, mRNA.

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CloneID	OXa62331
Clone ID Related Accession (Same CDS sequence)	XM_031905898.1
Accession Version	XM_031905898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCCAAG GCGGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG GCCAAGGCGG CTACCCCGGC CAAGAGGTCA 
CCAGCCAAGG CAGCTACCCC TGCCAAGAGG TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG 
AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG AGGTCTCCTG CTTCTACTTA TACAAAAGGA 
CGTTTCTCAA TTTCACGAAT TGATACACCA CCGCAAATAG ATGTCCATGC TCCTAACACT 
GAGAAAAATG AACTCTCTGC ACAAACACCC AGAAAATCAC GGAAGTCAAT ATCATTAAAG 
AAAACGCCAG GAAGAAGAAG TAGAAAACTT GAATCTTTTG AAATTTTGCG TTCCAGGCGA 
AGAAGTGGGG CAACTGAAGC TAACTTGCTT GTTGCAAAAT CATGGGCTGA TGTTGTAAGA 
AGCGGTGTTC CAAAAAATCA GAAGAAATCT GAAAAGCATG CTCGCAAAGC AGTACCTACC 
AAGAGAAAGC CAAAGCCAAA GACGCCAGCA CGAAGAATTA TTGACCGAAT TGGCACAGGA 
CATGCTGATT CTCCTGCCAC AATCCTTGTT GGAAGAGCTC ATACAAGAAC TGTCGACCTA 
ACTGGATATG TTCCTAAAAT TGTTCAAAAC CAAGCAATAA AAATCACCAA TGACCAAAAT 
GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT AATACCCCTG CACATGTGAA ACCAAGGAGA 
AGTGGTCGCT CAGGAGGCCT TCCGATGGAA ACTCCGCAAT CTATAATTGT TGATATGTCA 
CTGATGAAGA CTCCAGAGGA ATTAGGTGAA ATGGTGGTTT CTCCAATTAA TGGTAGCCCT 
GCTACAACAC AGAAGAAACA GTACAGTCGT GACGCAGTAT CTCGTCTTCT AAGTCCTGTG 
ACGCCAGCAT CAGAAGAACA GATATCCGAA GCTTCCAAAG ACACTCAGTT GGTTGTAAAA 
GAGACAGCCA CATTAAATGA AACCCGTTTT ATAAAAAAGG GTGCAAAGGC ACAGATGAAA 
AAAAGAGGAA CAATGGGTTT GACTGGTGTA AAAAGGATTA TGAGAACACC AAAACAAAAA 
GGAAAGCAGG TTATTGATCC TGTGGCGCTA AAGAAAATGT TGAGAACACC AAAACAAGTT 
GAATCACTTC CTGTAGAGTC CTCTGGCCCT AATGATCTAG TTGGACTGAA GGAAATCATG 
AAAACTCCAA AAGTAAAAGG GCAACCAGTG GAGGATATGA CTGGCATAGC ACGCATCATG 
AAGACCCCAA GACGCAAGCA ACAACCAGTA GAAGATATGG CTGGTATTAA ACGTATTATG 
AAGACTCCTA AACAGAAAGG GACGCCTGTT GAGGATATGA CCGGAATCAA GCGTATTATG 
ACGACCCCCA AGGAGAAAGG GCAACCCGTG GAGGACATGG TGGGAATCAA ACGTATTATG 
ACAACCCCCA AGGAGAAAGG GCAACCCGTG GAGGACATGG TGGGAATCAA ACGCATTATG 
AGAACCCCCA AGGAGAAAGG GCAACCTGTG GAGGATATGG AAGGTTTAAG TCAGATTATG 
AGAACACCAA CTGAAAATAC TCACCCAATA GAAGAAATAT TTGGGATGAA ACAACTAATA 
AAGACTCCTC CAAAGAGAGC ACTTGGTGTT AGAGAGGGCA CGTCTGGGAC ACCATCACAA 
TCTGGCAAAA AGGCTACTCT GTTTCAGACT AATCAAAAAT CGGCACAAGA AGAAGCATTG 
CCAAAACTTA CTTTGGAGAA AAATAATGAA AGCGAGGTTG CTGTACAAAC TGACAGAGAA 
ACTGAAAAAT CTAAACCAAC CCCCAAAAGA GGTCGACCTG CCAAGGCTAG TGTGAAGCTT 
GCTTCAGATG GTGGGAAGCC TGTTGATAAT GGCACATCTA CACATGAAAC ACACCCAGAA 
GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA CTGGCTAGTG ATGATAAATC CTCCACAGCT 
GCTGATAAAG CCACCCCAGC CAAACGAAGA GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT 
TCACCAGGTG GTGGAAATTC TGTTGAAAAC AACACATTCA GACATGAAAA AAATCCGGAA 
GAAGCCGCTC CTCTACAGGT ACTCTCCACT GAAAATACTT CATCCTTGGG TGATGATAAA 
GCTACTCCAG CTAAAAGAGG TCGTCCATGC AAACGTGGTA TGAGTAAGTC ACCAGCATCT 
AGTAATAAAG ATAAGTCTGT TGAAAAGAAT AGTACACCAG AGCATGAAAA GAACCTGGAA 
GAAAGCAATG AAGGAACATC TCTACAGGTA CAGACCATTG AAGATGCTTC TTCCACTAGT 
GCTGATAAAG CCACTCCGGC CAAAAGAAGA GGTCGTCCAG CTAAACGCAG CTTGAGTCAT 
TCACCAGGTT TAGAAAAACC TGTTGTAGAT GTTCCAGAAG ACCTAAGCAC CAGCAATGAG 
GTCTCTAATA TTCCTGAAGG ATCAAGTCAT ATCAATACAG ACCAAGAGAA CAATGTTGAT 
TTACCAATGG AAAAAGAGTC TGAACCGTTA AATCTAAATA TTGGAGCGAA AAAAGTCACT 
AAGAAATCTC AGAGAAAGAA AGTCACAGAA GAGTCTGTTC AAATAGCAGT GGAAAACTTG 
GTTGTTGAAC AAGATGATGA AAAATCTGCT CCCCAAAGAC GACGAGGAAG ACCTCAAAGG 
GGTGGTCTTT CATCTACCAC TGAAGTTTTA GACGAAGCAT TACCAGAAGA GCCTCCAACA 
AACAGACGAA CTGGCAGGGG CCGAAAGATT GCAGAAGTGG CAAAACCGCA ACAAGTCAGT 
ACAAGTGTTG AAGAATCTCA AGAAGTAAGA AACGAGGAAC TCTCAGCAAC TAAGACTCAG 
ACTAAGTCTA CCAGGCATCA AAAAGCTGTA TCTGAAGACT CTACAGTTAC AGCAACTAAG 
AAGCCACGTG GAAGGCAACC TAAAGTGGAC AGAGAACAGA AAGCTGCTGA GGAGGAAAAT 
ACTGTGGAGA AATCACTTAC AGAACTGCCA GGAGATTCAG CTTTAGTCAA CAATGTAACG 
CCATCTGTGA GAAGGGGTAG GCGAGCAAGA GGCAATGCAA ATGCTGTGAC TGAAACAGTC 
GTAACTGAAG CAAATTTGCC TGACTTGACA AACAATATCG TTTTACATAA AGAGCAAGAA 
AGCTCCAGTC AAGCATCTCC AGAAGCCAAA ACTCAAAGAG GAAAAAGGAA AAATCTTAAA 
AATACAAACG TTGATTCTGT AAGCAACTCT GCTGAAACAG ATGTTGCAAC ATTAGAATTA 
GAAGAGAGAC CTAACAAAAG ATCTACACGT GGTGTTAGAG GCACACAGCA AGTTGATGAG 
CTTAAAGAAC CAAAAGGAAA AAAAGCAAAA GTAGAACATG TGCCCAAACC TGCTGAACTA 
GTTCATATAC AGGAGAATCT AGCTAAAGAG AATGCATCTA GGGCGAAATC TGTGCAGTGG 
CACCCTCTTC TGACTGTTAA GGGGACACCT GAAAAGAATA CTGAAGAGAC TGTTAAGAAT 
CTTGCAGATG AAGAAGCTCC GGTCAGTGCA GTAAAATCTA GAGGAAGGAG GCGGCAAGAA 
GTAAAAGAAT CTGTTCCAGC AAAACGATCG CGTTCAAGAA GGGAGGAAAA TAATGCAGAG 
GCATTAACTT CTAGTAGTAC AACAGCTGAT GCAGTTCCTA TTGCTTTGGT TGCCAGTGAA 
AGTACTGAAA CTCCTAAGAA TAAGAGAGGA CGGAGGAAAG CAAACACACA ATTAGAGAAA 
GTTACTGATT CAGATCTTTC AGTGACAGAG GTTTCCTCTA AGCTAGACCT AGTGCCAGAG 
GTTGTAAACG AAAAGGCCGT AAACAAACGA AACACAGGTA AAAGAAACCT CAAGTCTTCA 
TCTGATAGTG ATTCTAGTAG TAAAGCTGTT GAACCAGTGC TGGAGACTAT TACCCAAAAG 
CGTGGACGAA GAGGAGCAGC AGCCAAAAGT CTAGATTCTG AGACTACTGA AGTTAAAACT 
GTTGAGTCTG CTGAAAATGT AACTTCAAAT AAAAGAAGGG GAAGAGCACC AAGTCAAAAA 
ACCAACAATC CTGAAACTAG CAAAACCACT ACTGAACCTG TTAATAAAAA TCAAGCAACT 
GAAGCTGTAG CCAAAAATAC AGATAATGCA GAAAGTCCTC CTAAATCAAG AAGAGGCTTA 
AAAAGAAAGG CAGCCACCAC TGATGTTCCG GAAGGCAACA CATCAGTAAG TGAAAATGGA 
CCCCGTGCCA ATGTAAGAGA AAGCACAAGA GGAGCTTCAA GGCAAAAAAC TGCAGCAAAA 
GAAAATCCAG TGGTTGTTGA AGAATCACCA GCAAAGAAAA AGAGAACAGA GCCATCTTCC 
AGTCCTGCTC AGAAAGGAGG AAAAACAAGA GCCACTGCAG CAAAACGGAA AAATGAGAAA 
CCAACAGAAG TTGTAAAGAC AAGGCAAACA TTGCGCACAC GGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761758.1
CDS	211..6498
Translation	MPLFGEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINLSTVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQTNPRSQSTGGNSLSSKLVEDEQPSIKGKASKLTRKSEGNVSRTTHSRLSLPISPHRRSSREKRDLSPFGELYEMLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905898.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905898.1

ORF Insert Sequence:

ATGCCACTTT TTGGGGAAAT TGTTGTGATA AAAAGGAATG GCACAGATGG TACCCATTTT 
CCACTTACTG CAACTACTTG TCTGTTTGGA AGAAAATCAG AATGTGATAT CCGCATTCAG 
CTACCACATG TCTCCAAAGA GCATTGCAGA GTTGAAGTCA AAGCAAACAA AGAGGTTTTT 
GTCATCAACT TAAGTACCGT CAATCCGACA CAGTTAAATG GAATAGCAGT TCAGCAACCT 
GAGGTCCTTA AACATGGAGA TGTAATTACT ATAATTGATC GTTCTTTCAG ATTTGAACAA 
CCACAGACGA ACCCTCGCAG TCAGTCCACA GGAGGAAATT CTCTAAGTAG CAAGTTGGTC 
GAAGATGAAC AGCCATCAAT CAAAGGTAAA GCGAGTAAGC TCACTCGGAA GTCAGAAGGA 
AATGTATCTA GGACCACCCA CTCTCGCCTC TCTCTTCCAA TATCACCACA TAGACGATCT 
TCAAGAGAGA AGAGAGATTT GTCACCTTTT GGTGAATTGT ACGAGATGCT TAAAAGTAAA 
GAGTTAAATC TAGAGCATGC AAAAACACCA GCAAAGCTAA ACATGGAGAA TGGAGATCAT 
ATCAGTACTC CTGTCACAAC TAGAAGGAGC GTTGGAAATG CTAAAGAAAC GCAAGAGTTG 
AGCATTGGAG AATTGCAGTT TGAAGGTCAG GAACTTCATG TTGAATCGGC AACCTTGTCA 
GAGAAAACTG CCAGTGCCTT AAAAAGTCCA TCTAGAGGGC GTCAGTCCAC TCTGTCAAAA 
CAAGAGCAAC AAAGGCTTAG TGGTAAAAGA GTCTCTCCTG CCAAATCTGA GCTGCTTCTT 
AAAGATACAG CTAGCTTTCA AGATAATGGG CAAAAACAGA CCAATGACAA GCTCACTCCA 
AATCGGAGAC GTAGTACTTC CAATGAACCT GCTCAAGATG TGCAAATGAC ACCTGGAAAA 
TCTGGAGGTT CCGTTTCACC TCGGTCCAGA AACATGTCTC CAAAATCAGC ATCTCCCAAA 
GGCTTGCACA AGAACGTTAA AGGATCAGCT AAAGTCTCAA ACCCACGACA AAAACGACGT 
AGTTCTGAGC TTGATTTGCC TGAGCCAACT CCAAAAAGAA AAAGGGTCTC ATTTGGAGGA 
CATCTAAGTC CAGAGCTTTT TGATAAAAGG CTTCCTCCCA ACTCTCCTCT TAAAAGAGGT 
GCAACACCTG CTAGGCGGAG CTTATCACTT GTCACGCCTC TTGCACTTAT GCGAAAGAGC 
TTTGGTGGTT TCAGGCAAAC CGCAATTAAG GAAACTTTTG AGCCTGGCAC TGACTTAGCA 
CTCTTCAAGG GATCTCCAGC CAAGAAGTCA CCATCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGGGGCAT CCCCGGCCAA GAGGTCGCCC 
GCCAAGGGGG CATCCCCGGC CAAGAGGTCG CCCGCCAAGG GGGCATCCCC GGCCAAGAGG 
TCGCCCGCCA AGGGGGCATC CCCGGCCAAG AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC 
AAGAGGTCGC CCGCCAAGGT AGCTACCCCG GCCAAGAGGT CGCCAGCCAA GGTAGCTACC 
CCGGCCAAGA GGTCGCCAGC AAAGGCGGCA TCCCCAGCCA AGAGGTCGCC GGCCAAGGCG 
GCTACCCCGG CCAAGAGGTC GCCGGCCAAG GCGGCTACCC CGGCCAAGAG GTCGCCGGCC 
AAGGCGGCTA CCCCGGCCAA GAGGTCGCCG GCCAAGGCGG CTACCCCGGC CAAGAGGTCA 
CCAGCCAAGG CAGCTACCCC TGCCAAGAGG TCGCCAGTCA AGGCAGCTAC CCCTGCCAAG 
AGGTCTCCTG CCTCAGCAAC CCCTGCCAAG AGGTCTCCTG CTTCTACTTA TACAAAAGGA 
CGTTTCTCAA TTTCACGAAT TGATACACCA CCGCAAATAG ATGTCCATGC TCCTAACACT 
GAGAAAAATG AACTCTCTGC ACAAACACCC AGAAAATCAC GGAAGTCAAT ATCATTAAAG 
AAAACGCCAG GAAGAAGAAG TAGAAAACTT GAATCTTTTG AAATTTTGCG TTCCAGGCGA 
AGAAGTGGGG CAACTGAAGC TAACTTGCTT GTTGCAAAAT CATGGGCTGA TGTTGTAAGA 
AGCGGTGTTC CAAAAAATCA GAAGAAATCT GAAAAGCATG CTCGCAAAGC AGTACCTACC 
AAGAGAAAGC CAAAGCCAAA GACGCCAGCA CGAAGAATTA TTGACCGAAT TGGCACAGGA 
CATGCTGATT CTCCTGCCAC AATCCTTGTT GGAAGAGCTC ATACAAGAAC TGTCGACCTA 
ACTGGATATG TTCCTAAAAT TGTTCAAAAC CAAGCAATAA AAATCACCAA TGACCAAAAT 
GAAAGCTTTA CAGGTTTGAG TGAGTTGTTT AATACCCCTG CACATGTGAA ACCAAGGAGA 
AGTGGTCGCT CAGGAGGCCT TCCGATGGAA ACTCCGCAAT CTATAATTGT TGATATGTCA 
CTGATGAAGA CTCCAGAGGA ATTAGGTGAA ATGGTGGTTT CTCCAATTAA TGGTAGCCCT 
GCTACAACAC AGAAGAAACA GTACAGTCGT GACGCAGTAT CTCGTCTTCT AAGTCCTGTG 
ACGCCAGCAT CAGAAGAACA GATATCCGAA GCTTCCAAAG ACACTCAGTT GGTTGTAAAA 
GAGACAGCCA CATTAAATGA AACCCGTTTT ATAAAAAAGG GTGCAAAGGC ACAGATGAAA 
AAAAGAGGAA CAATGGGTTT GACTGGTGTA AAAAGGATTA TGAGAACACC AAAACAAAAA 
GGAAAGCAGG TTATTGATCC TGTGGCGCTA AAGAAAATGT TGAGAACACC AAAACAAGTT 
GAATCACTTC CTGTAGAGTC CTCTGGCCCT AATGATCTAG TTGGACTGAA GGAAATCATG 
AAAACTCCAA AAGTAAAAGG GCAACCAGTG GAGGATATGA CTGGCATAGC ACGCATCATG 
AAGACCCCAA GACGCAAGCA ACAACCAGTA GAAGATATGG CTGGTATTAA ACGTATTATG 
AAGACTCCTA AACAGAAAGG GACGCCTGTT GAGGATATGA CCGGAATCAA GCGTATTATG 
ACGACCCCCA AGGAGAAAGG GCAACCCGTG GAGGACATGG TGGGAATCAA ACGTATTATG 
ACAACCCCCA AGGAGAAAGG GCAACCCGTG GAGGACATGG TGGGAATCAA ACGCATTATG 
AGAACCCCCA AGGAGAAAGG GCAACCTGTG GAGGATATGG AAGGTTTAAG TCAGATTATG 
AGAACACCAA CTGAAAATAC TCACCCAATA GAAGAAATAT TTGGGATGAA ACAACTAATA 
AAGACTCCTC CAAAGAGAGC ACTTGGTGTT AGAGAGGGCA CGTCTGGGAC ACCATCACAA 
TCTGGCAAAA AGGCTACTCT GTTTCAGACT AATCAAAAAT CGGCACAAGA AGAAGCATTG 
CCAAAACTTA CTTTGGAGAA AAATAATGAA AGCGAGGTTG CTGTACAAAC TGACAGAGAA 
ACTGAAAAAT CTAAACCAAC CCCCAAAAGA GGTCGACCTG CCAAGGCTAG TGTGAAGCTT 
GCTTCAGATG GTGGGAAGCC TGTTGATAAT GGCACATCTA CACATGAAAC ACACCCAGAA 
GAAAGCAGCA GAGAGGCTTC TCTCCAAGTA CTGGCTAGTG ATGATAAATC CTCCACAGCT 
GCTGATAAAG CCACCCCAGC CAAACGAAGA GGTCGTCCAT CCAAGTCCAG TTTGAGTGGT 
TCACCAGGTG GTGGAAATTC TGTTGAAAAC AACACATTCA GACATGAAAA AAATCCGGAA 
GAAGCCGCTC CTCTACAGGT ACTCTCCACT GAAAATACTT CATCCTTGGG TGATGATAAA 
GCTACTCCAG CTAAAAGAGG TCGTCCATGC AAACGTGGTA TGAGTAAGTC ACCAGCATCT 
AGTAATAAAG ATAAGTCTGT TGAAAAGAAT AGTACACCAG AGCATGAAAA GAACCTGGAA 
GAAAGCAATG AAGGAACATC TCTACAGGTA CAGACCATTG AAGATGCTTC TTCCACTAGT 
GCTGATAAAG CCACTCCGGC CAAAAGAAGA GGTCGTCCAG CTAAACGCAG CTTGAGTCAT 
TCACCAGGTT TAGAAAAACC TGTTGTAGAT GTTCCAGAAG ACCTAAGCAC CAGCAATGAG 
GTCTCTAATA TTCCTGAAGG ATCAAGTCAT ATCAATACAG ACCAAGAGAA CAATGTTGAT 
TTACCAATGG AAAAAGAGTC TGAACCGTTA AATCTAAATA TTGGAGCGAA AAAAGTCACT 
AAGAAATCTC AGAGAAAGAA AGTCACAGAA GAGTCTGTTC AAATAGCAGT GGAAAACTTG 
GTTGTTGAAC AAGATGATGA AAAATCTGCT CCCCAAAGAC GACGAGGAAG ACCTCAAAGG 
GGTGGTCTTT CATCTACCAC TGAAGTTTTA GACGAAGCAT TACCAGAAGA GCCTCCAACA 
AACAGACGAA CTGGCAGGGG CCGAAAGATT GCAGAAGTGG CAAAACCGCA ACAAGTCAGT 
ACAAGTGTTG AAGAATCTCA AGAAGTAAGA AACGAGGAAC TCTCAGCAAC TAAGACTCAG 
ACTAAGTCTA CCAGGCATCA AAAAGCTGTA TCTGAAGACT CTACAGTTAC AGCAACTAAG 
AAGCCACGTG GAAGGCAACC TAAAGTGGAC AGAGAACAGA AAGCTGCTGA GGAGGAAAAT 
ACTGTGGAGA AATCACTTAC AGAACTGCCA GGAGATTCAG CTTTAGTCAA CAATGTAACG 
CCATCTGTGA GAAGGGGTAG GCGAGCAAGA GGCAATGCAA ATGCTGTGAC TGAAACAGTC 
GTAACTGAAG CAAATTTGCC TGACTTGACA AACAATATCG TTTTACATAA AGAGCAAGAA 
AGCTCCAGTC AAGCATCTCC AGAAGCCAAA ACTCAAAGAG GAAAAAGGAA AAATCTTAAA 
AATACAAACG TTGATTCTGT AAGCAACTCT GCTGAAACAG ATGTTGCAAC ATTAGAATTA 
GAAGAGAGAC CTAACAAAAG ATCTACACGT GGTGTTAGAG GCACACAGCA AGTTGATGAG 
CTTAAAGAAC CAAAAGGAAA AAAAGCAAAA GTAGAACATG TGCCCAAACC TGCTGAACTA 
GTTCATATAC AGGAGAATCT AGCTAAAGAG AATGCATCTA GGGCGAAATC TGTGCAGTGG 
CACCCTCTTC TGACTGTTAA GGGGACACCT GAAAAGAATA CTGAAGAGAC TGTTAAGAAT 
CTTGCAGATG AAGAAGCTCC GGTCAGTGCA GTAAAATCTA GAGGAAGGAG GCGGCAAGAA 
GTAAAAGAAT CTGTTCCAGC AAAACGATCG CGTTCAAGAA GGGAGGAAAA TAATGCAGAG 
GCATTAACTT CTAGTAGTAC AACAGCTGAT GCAGTTCCTA TTGCTTTGGT TGCCAGTGAA 
AGTACTGAAA CTCCTAAGAA TAAGAGAGGA CGGAGGAAAG CAAACACACA ATTAGAGAAA 
GTTACTGATT CAGATCTTTC AGTGACAGAG GTTTCCTCTA AGCTAGACCT AGTGCCAGAG 
GTTGTAAACG AAAAGGCCGT AAACAAACGA AACACAGGTA AAAGAAACCT CAAGTCTTCA 
TCTGATAGTG ATTCTAGTAG TAAAGCTGTT GAACCAGTGC TGGAGACTAT TACCCAAAAG 
CGTGGACGAA GAGGAGCAGC AGCCAAAAGT CTAGATTCTG AGACTACTGA AGTTAAAACT 
GTTGAGTCTG CTGAAAATGT AACTTCAAAT AAAAGAAGGG GAAGAGCACC AAGTCAAAAA 
ACCAACAATC CTGAAACTAG CAAAACCACT ACTGAACCTG TTAATAAAAA TCAAGCAACT 
GAAGCTGTAG CCAAAAATAC AGATAATGCA GAAAGTCCTC CTAAATCAAG AAGAGGCTTA 
AAAAGAAAGG CAGCCACCAC TGATGTTCCG GAAGGCAACA CATCAGTAAG TGAAAATGGA 
CCCCGTGCCA ATGTAAGAGA AAGCACAAGA GGAGCTTCAA GGCAAAAAAC TGCAGCAAAA 
GAAAATCCAG TGGTTGTTGA AGAATCACCA GCAAAGAAAA AGAGAACAGA GCCATCTTCC 
AGTCCTGCTC AGAAAGGAGG AAAAACAAGA GCCACTGCAG CAAAACGGAA AAATGAGAAA 
CCAACAGAAG TTGTAAAGAC AAGGCAAACA TTGCGCACAC GGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mki67 ( XM_031905899.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761759.1 Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X14, mRNA.

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CloneID	OXa62332
Clone ID Related Accession (Same CDS sequence)	XM_031905899.1
Accession Version	XM_031905899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	proliferation marker protein Ki-67 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAAAA GTAAAGAGTT AAATCTAGAG CATGCAAAAA CACCAGCAAA GCTAAACATG 
GAGAATGGAG ATCATATCAG TACTCCTGTC ACAACTAGAA GGAGCGTTGG AAATGCTAAA 
GAAACGCAAG AGTTGAGCAT TGGAGAATTG CAGTTTGAAG GTCAGGAACT TCATGTTGAA 
TCGGCAACCT TGTCAGAGAA AACTGCCAGT GCCTTAAAAA GTCCATCTAG AGGGCGTCAG 
TCCACTCTGT CAAAACAAGA GCAACAAAGG CTTAGTGGTA AAAGAGTCTC TCCTGCCAAA 
TCTGAGCTGC TTCTTAAAGA TACAGCTAGC TTTCAAGATA ATGGGCAAAA ACAGACCAAT 
GACAAGCTCA CTCCAAATCG GAGACGTAGT ACTTCCAATG AACCTGCTCA AGATGTGCAA 
ATGACACCTG GAAAATCTGG AGGTTCCGTT TCACCTCGGT CCAGAAACAT GTCTCCAAAA 
TCAGCATCTC CCAAAGGCTT GCACAAGAAC GTTAAAGGAT CAGCTAAAGT CTCAAACCCA 
CGACAAAAAC GACGTAGTTC TGAGCTTGAT TTGCCTGAGC CAACTCCAAA AAGAAAAAGG 
GTCTCATTTG GAGGACATCT AAGTCCAGAG CTTTTTGATA AAAGGCTTCC TCCCAACTCT 
CCTCTTAAAA GAGGTGCAAC ACCTGCTAGG CGGAGCTTAT CACTTGTCAC GCCTCTTGCA 
CTTATGCGAA AGAGCTTTGG TGGTTTCAGG CAAACCGCAA TTAAGGAAAC TTTTGAGCCT 
GGCACTGACT TAGCACTCTT CAAGGGATCT CCAGCCAAGA AGTCACCATC CAAGAGGTCG 
CCCGCCAAGG GGGCATCCCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCAAAGG CGGCATCCCC AGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCGCCGG CAAAGGGGGC ATCCCCTGCC 
AGGAGGTCGC CCGCCAAGGC AGCTACCCCT GCCAGGAGGT CGCCCGCCAA GGCAGCTACC 
CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT ACCCCGGCCA AGAGGTCGCC GGCAAAGGGG 
GCATCCCCTG CCAAGAGGTC GCCGGCCAAG GCGGCATCCC CTGCCAGGAG GTCGCCGGCC 
AAGGCGGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCGG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CGGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCGGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGAGGTCG 
CCGGCCAAGG CGGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCAC CAGCCAAGGC AGCTACCCCT 
GCCAAGAGGT CGCCAGTCAA GGCAGCTACC CCTGCCAAGA GGTCTCCTGC CTCAGCAACC 
CCTGCCAAGA GGTCTCCTGC TTCTACTTAT ACAAAAGGAC GTTTCTCAAT TTCACGAATT 
GATACACCAC CGCAAATAGA TGTCCATGCT CCTAACACTG AGAAAAATGA ACTCTCTGCA 
CAAACACCCA GAAAATCACG GAAGTCAATA TCATTAAAGA AAACGCCAGG AAGAAGAAGT 
AGAAAACTTG AATCTTTTGA AATTTTGCGT TCCAGGCGAA GAAGTGGGGC AACTGAAGCT 
AACTTGCTTG TTGCAAAATC ATGGGCTGAT GTTGTAAGAA GCGGTGTTCC AAAAAATCAG 
AAGAAATCTG AAAAGCATGC TCGCAAAGCA GTACCTACCA AGAGAAAGCC AAAGCCAAAG 
ACGCCAGCAC GAAGAATTAT TGACCGAATT GGCACAGGAC ATGCTGATTC TCCTGCCACA 
ATCCTTGTTG GAAGAGCTCA TACAAGAACT GTCGACCTAA CTGGATATGT TCCTAAAATT 
GTTCAAAACC AAGCAATAAA AATCACCAAT GACCAAAATG AAAGCTTTAC AGGTTTGAGT 
GAGTTGTTTA ATACCCCTGC ACATGTGAAA CCAAGGAGAA GTGGTCGCTC AGGAGGCCTT 
CCGATGGAAA CTCCGCAATC TATAATTGTT GATATGTCAC TGATGAAGAC TCCAGAGGAA 
TTAGGTGAAA TGGTGGTTTC TCCAATTAAT GGTAGCCCTG CTACAACACA GAAGAAACAG 
TACAGTCGTG ACGCAGTATC TCGTCTTCTA AGTCCTGTGA CGCCAGCATC AGAAGAACAG 
ATATCCGAAG CTTCCAAAGA CACTCAGTTG GTTGTAAAAG AGACAGCCAC ATTAAATGAA 
ACCCGTTTTA TAAAAAAGGG TGCAAAGGCA CAGATGAAAA AAAGAGGAAC AATGGGTTTG 
ACTGGTGTAA AAAGGATTAT GAGAACACCA AAACAAAAAG GAAAGCAGGT TATTGATCCT 
GTGGCGCTAA AGAAAATGTT GAGAACACCA AAACAAGTTG AATCACTTCC TGTAGAGTCC 
TCTGGCCCTA ATGATCTAGT TGGACTGAAG GAAATCATGA AAACTCCAAA AGTAAAAGGG 
CAACCAGTGG AGGATATGAC TGGCATAGCA CGCATCATGA AGACCCCAAG ACGCAAGCAA 
CAACCAGTAG AAGATATGGC TGGTATTAAA CGTATTATGA AGACTCCTAA ACAGAAAGGG 
ACGCCTGTTG AGGATATGAC CGGAATCAAG CGTATTATGA CGACCCCCAA GGAGAAAGGG 
CAACCCGTGG AGGACATGGT GGGAATCAAA CGTATTATGA CAACCCCCAA GGAGAAAGGG 
CAACCCGTGG AGGACATGGT GGGAATCAAA CGCATTATGA GAACCCCCAA GGAGAAAGGG 
CAACCTGTGG AGGATATGGA AGGTTTAAGT CAGATTATGA GAACACCAAC TGAAAATACT 
CACCCAATAG AAGAAATATT TGGGATGAAA CAACTAATAA AGACTCCTCC AAAGAGAGCA 
CTTGGTGTTA GAGAGGGCAC GTCTGGGACA CCATCACAAT CTGGCAAAAA GGCTACTCTG 
TTTCAGACTA ATCAAAAATC GGCACAAGAA GAAGCATTGC CAAAACTTAC TTTGGAGAAA 
AATAATGAAA GCGAGGTTGC TGTACAAACT GACAGAGAAA CTGAAAAATC TAAACCAACC 
CCCAAAAGAG GTCGACCTGC CAAGGCTAGT GTGAAGCTTG CTTCAGATGG TGGGAAGCCT 
GTTGATAATG GCACATCTAC ACATGAAACA CACCCAGAAG AAAGCAGCAG AGAGGCTTCT 
CTCCAAGTAC TGGCTAGTGA TGATAAATCC TCCACAGCTG CTGATAAAGC CACCCCAGCC 
AAACGAAGAG GTCGTCCATC CAAGTCCAGT TTGAGTGGTT CACCAGGTGG TGGAAATTCT 
GTTGAAAACA ACACATTCAG ACATGAAAAA AATCCGGAAG AAGCCGCTCC TCTACAGGTA 
CTCTCCACTG AAAATACTTC ATCCTTGGGT GATGATAAAG CTACTCCAGC TAAAAGAGGT 
CGTCCATGCA AACGTGGTAT GAGTAAGTCA CCAGCATCTA GTAATAAAGA TAAGTCTGTT 
GAAAAGAATA GTACACCAGA GCATGAAAAG AACCTGGAAG AAAGCAATGA AGGAACATCT 
CTACAGGTAC AGACCATTGA AGATGCTTCT TCCACTAGTG CTGATAAAGC CACTCCGGCC 
AAAAGAAGAG GTCGTCCAGC TAAACGCAGC TTGAGTCATT CACCAGGTTT AGAAAAACCT 
GTTGTAGATG TTCCAGAAGA CCTAAGCACC AGCAATGAGG TCTCTAATAT TCCTGAAGGA 
TCAAGTCATA TCAATACAGA CCAAGAGAAC AATGTTGATT TACCAATGGA AAAAGAGTCT 
GAACCGTTAA ATCTAAATAT TGGAGCGAAA AAAGTCACTA AGAAATCTCA GAGAAAGAAA 
GTCACAGAAG AGTCTGTTCA AATAGCAGTG GAAAACTTGG TTGTTGAACA AGATGATGAA 
AAATCTGCTC CCCAAAGACG ACGAGGAAGA CCTCAAAGGG GTGGTCTTTC ATCTACCACT 
GAAGTTTTAG ACGAAGCATT ACCAGAAGAG CCTCCAACAA ACAGACGAAC TGGCAGGGGC 
CGAAAGATTG CAGAAGTGGC AAAACCGCAA CAAGTCAGTA CAAGTGTTGA AGAATCTCAA 
GAAGTAAGAA ACGAGGAACT CTCAGCAACT AAGACTCAGA CTAAGTCTAC CAGGCATCAA 
AAAGCTGTAT CTGAAGACTC TACAGTTACA GCAACTAAGA AGCCACGTGG AAGGCAACCT 
AAAGTGGACA GAGAACAGAA AGCTGCTGAG GAGGAAAATA CTGTGGAGAA ATCACTTACA 
GAACTGCCAG GAGATTCAGC TTTAGTCAAC AATGTAACGC CATCTGTGAG AAGGGGTAGG 
CGAGCAAGAG GCAATGCAAA TGCTGTGACT GAAACAGTCG TAACTGAAGC AAATTTGCCT 
GACTTGACAA ACAATATCGT TTTACATAAA GAGCAAGAAA GCTCCAGTCA AGCATCTCCA 
GAAGCCAAAA CTCAAAGAGG AAAAAGGAAA AATCTTAAAA ATACAAACGT TGATTCTGTA 
AGCAACTCTG CTGAAACAGA TGTTGCAACA TTAGAATTAG AAGAGAGACC TAACAAAAGA 
TCTACACGTG GTGTTAGAGG CACACAGCAA GTTGATGAGC TTAAAGAACC AAAAGGAAAA 
AAAGCAAAAG TAGAACATGT GCCCAAACCT GCTGAACTAG TTCATATACA GGAGAATCTA 
GCTAAAGAGA ATGCATCTAG GGCGAAATCT GTGCAGTGGC ACCCTCTTCT GACTGTTAAG 
GGGACACCTG AAAAGAATAC TGAAGAGACT GTTAAGAATC TTGCAGATGA AGAAGCTCCG 
GTCAGTGCAG TAAAATCTAG AGGAAGGAGG CGGCAAGAAG TAAAAGAATC TGTTCCAGCA 
AAACGATCGC GTTCAAGAAG GGAGGAAAAT AATGCAGAGG CATTAACTTC TAGTAGTACA 
ACAGCTGATG CAGTTCCTAT TGCTTTGGTT GCCAGTGAAA GTACTGAAAC TCCTAAGAAT 
AAGAGAGGAC GGAGGAAAGC AAACACACAA TTAGAGAAAG TTACTGATTC AGATCTTTCA 
GTGACAGAGG TTTCCTCTAA GCTAGACCTA GTGCCAGAGG TTGTAAACGA AAAGGCCGTA 
AACAAACGAA ACACAGGTAA AAGAAACCTC AAGTCTTCAT CTGATAGTGA TTCTAGTAGT 
AAAGCTGTTG AACCAGTGCT GGAGACTATT ACCCAAAAGC GTGGACGAAG AGGAGCAGCA 
GCCAAAAGTC TAGATTCTGA GACTACTGAA GTTAAAACTG TTGAGTCTGC TGAAAATGTA 
ACTTCAAATA AAAGAAGGGG AAGAGCACCA AGTCAAAAAA CCAACAATCC TGAAACTAGC 
AAAACCACTA CTGAACCTGT TAATAAAAAT CAAGCAACTG AAGCTGTAGC CAAAAATACA 
GATAATGCAG AAAGTCCTCC TAAATCAAGA AGAGGCTTAA AAAGAAAGGC AGCCACCACT 
GATGTTCCGG AAGGCAACAC ATCAGTAAGT GAAAATGGAC CCCGTGCCAA TGTAAGAGAA 
AGCACAAGAG GAGCTTCAAG GCAAAAAACT GCAGCAAAAG AAAATCCAGT GGTTGTTGAA 
GAATCACCAG CAAAGAAAAA GAGAACAGAG CCATCTTCCA GTCCTGCTCA GAAAGGAGGA 
AAAACAAGAG CCACTGCAGC AAAACGGAAA AATGAGAAAC CAACAGAAGT TGTAAAGACA 
AGGCAAACAT TGCGCACACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761759.1
CDS	427..6633
Translation	MLKSKELNLEHAKTPAKLNMENGDHISTPVTTRRSVGNAKETQELSIGELQFEGQELHVESATLSEKTASALKSPSRGRQSTLSKQEQQRLSGKRVSPAKSELLLKDTASFQDNGQKQTNDKLTPNRRRSTSNEPAQDVQMTPGKSGGSVSPRSRNMSPKSASPKGLHKNVKGSAKVSNPRQKRRSSELDLPEPTPKRKRVSFGGHLSPELFDKRLPPNSPLKRGATPARRSLSLVTPLALMRKSFGGFRQTAIKETFEPGTDLALFKGSPAKKSPSKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKGASPAKRSPAKVATPAKRSPAKVATPAKRSPAKAASPAKRSPAKAATPAKRSPAKGASPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKGASPAKRSPAKAASPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPARRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPAKAATPAKRSPVKAATPAKRSPASATPAKRSPASTYTKGRFSISRIDTPPQIDVHAPNTEKNELSAQTPRKSRKSISLKKTPGRRSRKLESFEILRSRRRSGATEANLLVAKSWADVVRSGVPKNQKKSEKHARKAVPTKRKPKPKTPARRIIDRIGTGHADSPATILVGRAHTRTVDLTGYVPKIVQNQAIKITNDQNESFTGLSELFNTPAHVKPRRSGRSGGLPMETPQSIIVDMSLMKTPEELGEMVVSPINGSPATTQKKQYSRDAVSRLLSPVTPASEEQISEASKDTQLVVKETATLNETRFIKKGAKAQMKKRGTMGLTGVKRIMRTPKQKGKQVIDPVALKKMLRTPKQVESLPVESSGPNDLVGLKEIMKTPKVKGQPVEDMTGIARIMKTPRRKQQPVEDMAGIKRIMKTPKQKGTPVEDMTGIKRIMTTPKEKGQPVEDMVGIKRIMTTPKEKGQPVEDMVGIKRIMRTPKEKGQPVEDMEGLSQIMRTPTENTHPIEEIFGMKQLIKTPPKRALGVREGTSGTPSQSGKKATLFQTNQKSAQEEALPKLTLEKNNESEVAVQTDRETEKSKPTPKRGRPAKASVKLASDGGKPVDNGTSTHETHPEESSREASLQVLASDDKSSTAADKATPAKRRGRPSKSSLSGSPGGGNSVENNTFRHEKNPEEAAPLQVLSTENTSSLGDDKATPAKRGRPCKRGMSKSPASSNKDKSVEKNSTPEHEKNLEESNEGTSLQVQTIEDASSTSADKATPAKRRGRPAKRSLSHSPGLEKPVVDVPEDLSTSNEVSNIPEGSSHINTDQENNVDLPMEKESEPLNLNIGAKKVTKKSQRKKVTEESVQIAVENLVVEQDDEKSAPQRRRGRPQRGGLSSTTEVLDEALPEEPPTNRRTGRGRKIAEVAKPQQVSTSVEESQEVRNEELSATKTQTKSTRHQKAVSEDSTVTATKKPRGRQPKVDREQKAAEEENTVEKSLTELPGDSALVNNVTPSVRRGRRARGNANAVTETVVTEANLPDLTNNIVLHKEQESSSQASPEAKTQRGKRKNLKNTNVDSVSNSAETDVATLELEERPNKRSTRGVRGTQQVDELKEPKGKKAKVEHVPKPAELVHIQENLAKENASRAKSVQWHPLLTVKGTPEKNTEETVKNLADEEAPVSAVKSRGRRRQEVKESVPAKRSRSRREENNAEALTSSSTTADAVPIALVASESTETPKNKRGRRKANTQLEKVTDSDLSVTEVSSKLDLVPEVVNEKAVNKRNTGKRNLKSSSDSDSSSKAVEPVLETITQKRGRRGAAAKSLDSETTEVKTVESAENVTSNKRRGRAPSQKTNNPETSKTTTEPVNKNQATEAVAKNTDNAESPPKSRRGLKRKAATTDVPEGNTSVSENGPRANVRESTRGASRQKTAAKENPVVVEESPAKKKRTEPSSSPAQKGGKTRATAAKRKNEKPTEVVKTRQTLRTRK

Target ORF information:

RefSeq Version	XM_031905899.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis marker of proliferation Ki-67 (mki67), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905899.1

ORF Insert Sequence:

ATGCTTAAAA GTAAAGAGTT AAATCTAGAG CATGCAAAAA CACCAGCAAA GCTAAACATG 
GAGAATGGAG ATCATATCAG TACTCCTGTC ACAACTAGAA GGAGCGTTGG AAATGCTAAA 
GAAACGCAAG AGTTGAGCAT TGGAGAATTG CAGTTTGAAG GTCAGGAACT TCATGTTGAA 
TCGGCAACCT TGTCAGAGAA AACTGCCAGT GCCTTAAAAA GTCCATCTAG AGGGCGTCAG 
TCCACTCTGT CAAAACAAGA GCAACAAAGG CTTAGTGGTA AAAGAGTCTC TCCTGCCAAA 
TCTGAGCTGC TTCTTAAAGA TACAGCTAGC TTTCAAGATA ATGGGCAAAA ACAGACCAAT 
GACAAGCTCA CTCCAAATCG GAGACGTAGT ACTTCCAATG AACCTGCTCA AGATGTGCAA 
ATGACACCTG GAAAATCTGG AGGTTCCGTT TCACCTCGGT CCAGAAACAT GTCTCCAAAA 
TCAGCATCTC CCAAAGGCTT GCACAAGAAC GTTAAAGGAT CAGCTAAAGT CTCAAACCCA 
CGACAAAAAC GACGTAGTTC TGAGCTTGAT TTGCCTGAGC CAACTCCAAA AAGAAAAAGG 
GTCTCATTTG GAGGACATCT AAGTCCAGAG CTTTTTGATA AAAGGCTTCC TCCCAACTCT 
CCTCTTAAAA GAGGTGCAAC ACCTGCTAGG CGGAGCTTAT CACTTGTCAC GCCTCTTGCA 
CTTATGCGAA AGAGCTTTGG TGGTTTCAGG CAAACCGCAA TTAAGGAAAC TTTTGAGCCT 
GGCACTGACT TAGCACTCTT CAAGGGATCT CCAGCCAAGA AGTCACCATC CAAGAGGTCG 
CCCGCCAAGG GGGCATCCCC GGCCAAGAGG TCGCCCGCCA AGGGGGCATC CCCGGCCAAG 
AGGTCGCCCG CCAAGGGGGC ATCCCCGGCC AAGAGGTCGC CCGCCAAGGG GGCATCCCCG 
GCCAAGAGGT CGCCCGCCAA GGGGGCATCC CCGGCCAAGA GGTCGCCCGC CAAGGGGGCA 
TCCCCGGCCA AGAGGTCGCC CGCCAAGGGG GCATCCCCGG CCAAGAGGTC GCCCGCCAAG 
GGGGCATCCC CGGCCAAGAG GTCGCCCGCC AAGGTAGCTA CCCCGGCCAA GAGGTCGCCA 
GCCAAGGTAG CTACCCCGGC CAAGAGGTCG CCAGCAAAGG CGGCATCCCC AGCCAAGAGG 
TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG AGGTCGCCGG CAAAGGGGGC ATCCCCTGCC 
AGGAGGTCGC CCGCCAAGGC AGCTACCCCT GCCAGGAGGT CGCCCGCCAA GGCAGCTACC 
CCTGCCAGGA GGTCGCCGGC CAAGGCAGCT ACCCCGGCCA AGAGGTCGCC GGCAAAGGGG 
GCATCCCCTG CCAAGAGGTC GCCGGCCAAG GCGGCATCCC CTGCCAGGAG GTCGCCGGCC 
AAGGCGGCTA CCCCTGCCAG GAGGTCGCCG GCCAAGGCGG CTACCCCTGC CAGGAGGTCG 
CCGGCCAAGG CGGCTACCCC TGCCAGGAGG TCGCCGGCCA AGGCGGCTAC CCCTGCCAGG 
AGGTCGCCGG CCAAGGCGGC TACCCCTGCC AAGAGGTCGC CGGCCAAGGC GGCTACCCCG 
GCCAAGAGGT CGCCGGCCAA GGCGGCTACC CCGGCCAAGA GGTCGCCGGC CAAGAGGTCG 
CCGGCCAAGG CGGCTACCCC GGCCAAGAGG TCGCCGGCCA AGGCGGCTAC CCCGGCCAAG 
AGGTCGCCGG CCAAGGCGGC TACCCCGGCC AAGAGGTCAC CAGCCAAGGC AGCTACCCCT 
GCCAAGAGGT CGCCAGTCAA GGCAGCTACC CCTGCCAAGA GGTCTCCTGC CTCAGCAACC 
CCTGCCAAGA GGTCTCCTGC TTCTACTTAT ACAAAAGGAC GTTTCTCAAT TTCACGAATT 
GATACACCAC CGCAAATAGA TGTCCATGCT CCTAACACTG AGAAAAATGA ACTCTCTGCA 
CAAACACCCA GAAAATCACG GAAGTCAATA TCATTAAAGA AAACGCCAGG AAGAAGAAGT 
AGAAAACTTG AATCTTTTGA AATTTTGCGT TCCAGGCGAA GAAGTGGGGC AACTGAAGCT 
AACTTGCTTG TTGCAAAATC ATGGGCTGAT GTTGTAAGAA GCGGTGTTCC AAAAAATCAG 
AAGAAATCTG AAAAGCATGC TCGCAAAGCA GTACCTACCA AGAGAAAGCC AAAGCCAAAG 
ACGCCAGCAC GAAGAATTAT TGACCGAATT GGCACAGGAC ATGCTGATTC TCCTGCCACA 
ATCCTTGTTG GAAGAGCTCA TACAAGAACT GTCGACCTAA CTGGATATGT TCCTAAAATT 
GTTCAAAACC AAGCAATAAA AATCACCAAT GACCAAAATG AAAGCTTTAC AGGTTTGAGT 
GAGTTGTTTA ATACCCCTGC ACATGTGAAA CCAAGGAGAA GTGGTCGCTC AGGAGGCCTT 
CCGATGGAAA CTCCGCAATC TATAATTGTT GATATGTCAC TGATGAAGAC TCCAGAGGAA 
TTAGGTGAAA TGGTGGTTTC TCCAATTAAT GGTAGCCCTG CTACAACACA GAAGAAACAG 
TACAGTCGTG ACGCAGTATC TCGTCTTCTA AGTCCTGTGA CGCCAGCATC AGAAGAACAG 
ATATCCGAAG CTTCCAAAGA CACTCAGTTG GTTGTAAAAG AGACAGCCAC ATTAAATGAA 
ACCCGTTTTA TAAAAAAGGG TGCAAAGGCA CAGATGAAAA AAAGAGGAAC AATGGGTTTG 
ACTGGTGTAA AAAGGATTAT GAGAACACCA AAACAAAAAG GAAAGCAGGT TATTGATCCT 
GTGGCGCTAA AGAAAATGTT GAGAACACCA AAACAAGTTG AATCACTTCC TGTAGAGTCC 
TCTGGCCCTA ATGATCTAGT TGGACTGAAG GAAATCATGA AAACTCCAAA AGTAAAAGGG 
CAACCAGTGG AGGATATGAC TGGCATAGCA CGCATCATGA AGACCCCAAG ACGCAAGCAA 
CAACCAGTAG AAGATATGGC TGGTATTAAA CGTATTATGA AGACTCCTAA ACAGAAAGGG 
ACGCCTGTTG AGGATATGAC CGGAATCAAG CGTATTATGA CGACCCCCAA GGAGAAAGGG 
CAACCCGTGG AGGACATGGT GGGAATCAAA CGTATTATGA CAACCCCCAA GGAGAAAGGG 
CAACCCGTGG AGGACATGGT GGGAATCAAA CGCATTATGA GAACCCCCAA GGAGAAAGGG 
CAACCTGTGG AGGATATGGA AGGTTTAAGT CAGATTATGA GAACACCAAC TGAAAATACT 
CACCCAATAG AAGAAATATT TGGGATGAAA CAACTAATAA AGACTCCTCC AAAGAGAGCA 
CTTGGTGTTA GAGAGGGCAC GTCTGGGACA CCATCACAAT CTGGCAAAAA GGCTACTCTG 
TTTCAGACTA ATCAAAAATC GGCACAAGAA GAAGCATTGC CAAAACTTAC TTTGGAGAAA 
AATAATGAAA GCGAGGTTGC TGTACAAACT GACAGAGAAA CTGAAAAATC TAAACCAACC 
CCCAAAAGAG GTCGACCTGC CAAGGCTAGT GTGAAGCTTG CTTCAGATGG TGGGAAGCCT 
GTTGATAATG GCACATCTAC ACATGAAACA CACCCAGAAG AAAGCAGCAG AGAGGCTTCT 
CTCCAAGTAC TGGCTAGTGA TGATAAATCC TCCACAGCTG CTGATAAAGC CACCCCAGCC 
AAACGAAGAG GTCGTCCATC CAAGTCCAGT TTGAGTGGTT CACCAGGTGG TGGAAATTCT 
GTTGAAAACA ACACATTCAG ACATGAAAAA AATCCGGAAG AAGCCGCTCC TCTACAGGTA 
CTCTCCACTG AAAATACTTC ATCCTTGGGT GATGATAAAG CTACTCCAGC TAAAAGAGGT 
CGTCCATGCA AACGTGGTAT GAGTAAGTCA CCAGCATCTA GTAATAAAGA TAAGTCTGTT 
GAAAAGAATA GTACACCAGA GCATGAAAAG AACCTGGAAG AAAGCAATGA AGGAACATCT 
CTACAGGTAC AGACCATTGA AGATGCTTCT TCCACTAGTG CTGATAAAGC CACTCCGGCC 
AAAAGAAGAG GTCGTCCAGC TAAACGCAGC TTGAGTCATT CACCAGGTTT AGAAAAACCT 
GTTGTAGATG TTCCAGAAGA CCTAAGCACC AGCAATGAGG TCTCTAATAT TCCTGAAGGA 
TCAAGTCATA TCAATACAGA CCAAGAGAAC AATGTTGATT TACCAATGGA AAAAGAGTCT 
GAACCGTTAA ATCTAAATAT TGGAGCGAAA AAAGTCACTA AGAAATCTCA GAGAAAGAAA 
GTCACAGAAG AGTCTGTTCA AATAGCAGTG GAAAACTTGG TTGTTGAACA AGATGATGAA 
AAATCTGCTC CCCAAAGACG ACGAGGAAGA CCTCAAAGGG GTGGTCTTTC ATCTACCACT 
GAAGTTTTAG ACGAAGCATT ACCAGAAGAG CCTCCAACAA ACAGACGAAC TGGCAGGGGC 
CGAAAGATTG CAGAAGTGGC AAAACCGCAA CAAGTCAGTA CAAGTGTTGA AGAATCTCAA 
GAAGTAAGAA ACGAGGAACT CTCAGCAACT AAGACTCAGA CTAAGTCTAC CAGGCATCAA 
AAAGCTGTAT CTGAAGACTC TACAGTTACA GCAACTAAGA AGCCACGTGG AAGGCAACCT 
AAAGTGGACA GAGAACAGAA AGCTGCTGAG GAGGAAAATA CTGTGGAGAA ATCACTTACA 
GAACTGCCAG GAGATTCAGC TTTAGTCAAC AATGTAACGC CATCTGTGAG AAGGGGTAGG 
CGAGCAAGAG GCAATGCAAA TGCTGTGACT GAAACAGTCG TAACTGAAGC AAATTTGCCT 
GACTTGACAA ACAATATCGT TTTACATAAA GAGCAAGAAA GCTCCAGTCA AGCATCTCCA 
GAAGCCAAAA CTCAAAGAGG AAAAAGGAAA AATCTTAAAA ATACAAACGT TGATTCTGTA 
AGCAACTCTG CTGAAACAGA TGTTGCAACA TTAGAATTAG AAGAGAGACC TAACAAAAGA 
TCTACACGTG GTGTTAGAGG CACACAGCAA GTTGATGAGC TTAAAGAACC AAAAGGAAAA 
AAAGCAAAAG TAGAACATGT GCCCAAACCT GCTGAACTAG TTCATATACA GGAGAATCTA 
GCTAAAGAGA ATGCATCTAG GGCGAAATCT GTGCAGTGGC ACCCTCTTCT GACTGTTAAG 
GGGACACCTG AAAAGAATAC TGAAGAGACT GTTAAGAATC TTGCAGATGA AGAAGCTCCG 
GTCAGTGCAG TAAAATCTAG AGGAAGGAGG CGGCAAGAAG TAAAAGAATC TGTTCCAGCA 
AAACGATCGC GTTCAAGAAG GGAGGAAAAT AATGCAGAGG CATTAACTTC TAGTAGTACA 
ACAGCTGATG CAGTTCCTAT TGCTTTGGTT GCCAGTGAAA GTACTGAAAC TCCTAAGAAT 
AAGAGAGGAC GGAGGAAAGC AAACACACAA TTAGAGAAAG TTACTGATTC AGATCTTTCA 
GTGACAGAGG TTTCCTCTAA GCTAGACCTA GTGCCAGAGG TTGTAAACGA AAAGGCCGTA 
AACAAACGAA ACACAGGTAA AAGAAACCTC AAGTCTTCAT CTGATAGTGA TTCTAGTAGT 
AAAGCTGTTG AACCAGTGCT GGAGACTATT ACCCAAAAGC GTGGACGAAG AGGAGCAGCA 
GCCAAAAGTC TAGATTCTGA GACTACTGAA GTTAAAACTG TTGAGTCTGC TGAAAATGTA 
ACTTCAAATA AAAGAAGGGG AAGAGCACCA AGTCAAAAAA CCAACAATCC TGAAACTAGC 
AAAACCACTA CTGAACCTGT TAATAAAAAT CAAGCAACTG AAGCTGTAGC CAAAAATACA 
GATAATGCAG AAAGTCCTCC TAAATCAAGA AGAGGCTTAA AAAGAAAGGC AGCCACCACT 
GATGTTCCGG AAGGCAACAC ATCAGTAAGT GAAAATGGAC CCCGTGCCAA TGTAAGAGAA 
AGCACAAGAG GAGCTTCAAG GCAAAAAACT GCAGCAAAAG AAAATCCAGT GGTTGTTGAA 
GAATCACCAG CAAAGAAAAA GAGAACAGAG CCATCTTCCA GTCCTGCTCA GAAAGGAGGA 
AAAACAAGAG CCACTGCAGC AAAACGGAAA AATGAGAAAC CAACAGAAGT TGTAAAGACA 
AGGCAAACAT TGCGCACACG GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mki67
Entrez Gene ID	779458
Full Name	marker of proliferation Ki-67
Synonyms	Ki-67,kia
General protein information	Preferred Names marker of proliferation Ki-67 Names antigen KI-67 antigen identified by monoclonal antibody Ki-67
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mki67 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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