kdm6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kdm6b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm6b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa53612 XM_031898016.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53612 XM_031898017.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53612 XM_031898015.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53613 XM_031898018.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53614 XM_031898019.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53614 XM_031898020.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa53615 XM_031898021.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa20376 NM_001278756.1
Latest version!
Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa53612
    Clone ID Related Accession (Same CDS sequence) XM_031898015.1 , XM_031898016.1 , XM_031898017.1
    Accession Version XM_031898015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753875.1
    CDS542..5764
    Translation

    Target ORF information:

    RefSeq Version XM_031898015.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53612
    Clone ID Related Accession (Same CDS sequence) XM_031898015.1 , XM_031898016.1 , XM_031898017.1
    Accession Version XM_031898016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753876.1
    CDS621..5843
    Translation

    Target ORF information:

    RefSeq Version XM_031898016.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53612
    Clone ID Related Accession (Same CDS sequence) XM_031898015.1 , XM_031898016.1 , XM_031898017.1
    Accession Version XM_031898017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753877.1
    CDS904..6126
    Translation

    Target ORF information:

    RefSeq Version XM_031898017.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC
    AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG
    CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG
    GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG
    ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG
    GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC
    AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC
    GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC
    GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG
    CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG
    AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA
    ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC
    TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA
    GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA
    TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC
    CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG
    ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53613
    Clone ID Related Accession (Same CDS sequence) XM_031898018.1
    Accession Version XM_031898018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGAAGA GACATTTCGG AGCTAAACGG
    TGTCTCCCGC AGCTGAAGCG CCCGGTGCCC CCCTCAGACT CTTCCGCACT CCAGCTGAGC
    CCACACAATC CCCATGGAGG GGAGGAGCAA CCGATGAAAC GGAGGAGGAG CCGGAGCCCC
    GAACAGCAAA GGAGGGGGAA CCCTGGCCTG CACTCTCCTC CAGCCTCAGG GGGTTCTGGG
    AGTTTGTACC ATCAGGCACC CGTCAAGCCC AGTGTTTGGA ATCCACTCAC CCATGTGCCC
    TGGGCTGAGC GCAGGGAGCG GCAGGAACCC AGACCCCCCC ACGCGCCGTC TTACCCACCA
    TATTGCCCTC CACGCCCTCT ACCGCCTCCA CCACCACTTC ACCAGCAGCC CCGCCCTCCA
    AGTGACCTTA GTGAGGCTCG GGGGCAGAGG TGTGCAGAGG CCGTCCCCCC AGCACCGCCC
    CCTCTCCACG TGCCTTACGA TCAGAGAAGA CCCCGCGCCA CCCCGCCGAC TACCCCCAGG
    ACCGATCTCA CCACTTCCTC TGATCGCTAC CAGAATTCCG CAGGCAGCCC GTCTAGAATC
    TGCCCTCCTC TTGTAGAGCA GCTGGACCCC CCACAAACTA TATGTCAACC CCAGCTACCA
    GCTGTCTCTC GACCTCCTCC TCCTCTGCAA CAGGGATGGC TGCCCCCCCC AGCCAAAGAG
    GAGATGAAGA AGGGAGACCC ACTAGAGGAC ATTCTGTATG GAGATGGACC TACAAAGGTT
    AAACAGGAAG GGTTTTACCC ATCCCGTGAC ACCCAAAGAG AGGGCAATGT CTACAACCAC
    CAACCTCAGG GCATTACCAC CAGCAAAGCC AGGTCTGAGG CCTACCAGCC TGGAAGCCTG
    GATCACGTAC TGCAGAAGAT GGATGAATCT GGGTTAATTG CCCCAAAGAA GGAGGAAGAG
    ATTAAACAGG AAGTTAGAAA TCATCCATTG ACCCCAGAAC TGGAGGTTGG AGGCAAACAA
    TCAAGCCATG GACTCTCTAG AGCTGAGGGC AGCTATTCTG GTGGGAAGGG GTCACCAGAA
    GGCGCGGATG AAACGTCTTT CTGCCCCTCT TTGGCCCCCC ACCACAAAGA TACCTCAGGG
    AGCCTCCCGG TTCCAGGCTC AGTTGCATTA CCAGCCAAGC CAATGCCTAA CTTGACTCGC
    AGGCCACTAA GTCCGAACTC GGCCTTCTCG GAGAGAACGT CGGTGTGCGG ACAGGAGAAG
    GAGCAACCCA CTGGATGCAA CCAGCTCTTT GAATTCCCCG GGGGGCAGTT GCACGAGCCC
    TTTGAGGAAG CCGCGGAGTT TGCAAAAATA TTACCGGACG GGCTTGACGA CATCATGAAA
    ATGCTTGACG AGTCGATAGA AAAGGAAGAG GAAGGGGTTG GGAACGCCAG TGGGATGGAC
    GATAGTTCCT CCACTACATC CAGCAGTCCA CCAGCAGAGA CCATGCCAGC ATCCAGCCTA
    GTGAGCTCAA ATCAGACCAC TGTTGTGAAA AGCCCACCGC CCAATGCCAA AACAGCGGAA
    GTCCGGCCAA GTGACACCTT CCCCTATCAT GGCCCTAAGA AAGCCATGCT GGAGCAAGAA
    GCAAGCAAGG AGGCAAAGAC TGCGCAGCAC AGGGGAGGCA GCGTTCTGAA ATCACTCGCA
    AATGTTCTAG AGGGGCAGAA GTATTGCTAC CGAGGCAGCT CTGGGGCGAA GTCCGCAGGA
    AACCCGGCAT ACTTCACTGG TGCTATGGTG AGCAAGCGTA ATGTTGACCC ATACGCTACG
    CAATCTACCT CACACCGGGC CTGGGACACC AAGCCAGGGA CGCATGTTGG GGCAGCCCCT
    ACCTCAGAGG CTCTTAAGCC AGGGGTGATT GAGAAAAGGC CAGAGGTACG GACAGAGACG
    GACATTCTGG AGGACATAGC GAGGGCTTGT GAGACTTTGG TTGAGGGTGC AAAGGGGTCG
    GCGGCAAGCA GGACGGGAAA CGAGAGAGCA GTTGAGAAAG AGCCCAAAAA GTCTGAAGGG
    ATTGTGGACA GGAAGCCAGA TTTACAGTGG GGCATAGAAA AACGTGACAT CCATTGGGGG
    GCAAATAAAC TAGAAGGACC AGTTAAGCAG AAAGTAGATT TCAAGTGGAG AAGCGATACC
    CAGAAAGAAG TGGTTGAGAA GAAGCCAGAG GCTCCCAGGG CTACTGGCCA ACAGGACTAT
    GTTGAGAAGA AACATGGGGC AGACAAACCC CGCGTCCCCG TTGACAGGAA GCCTGAGACT
    TTGCAGAAGG CAGATGTACA GGAAGGAGTT GCTGAAATAA AGCAGGAAAC ATCTTATGGC
    ACAATCAGGC ATGAAGTGGC AGAGAGGCTG GAGGCGGCTC TTGAGAGAGA ATCCAAGAAG
    TCGGTGAAGG GAGCAGAGAA GAAATGTCTC CCAGGTCCAG CTGACTCCCC CTCAGGTACA
    GCCCGGGAAG ATCCCTCTAA AATACACGGT GCTAATGTCA AGGAGGCTGT AAAGGAGGCG
    ACAGCTAAGC GGCTGCAAGG GGTAAATGAC AGAGAGTCAC CAAAAAAGAA CACCGATTCT
    TTGGCCCAGG GAGGGGTCAG TAAGAACGTG GCGGCTGAAG TGGCAAAACC GAATGCCGAT
    AGCAAGGACA CTAAGTCATC TGAGGAAGCA CCGATGCCAA CAATGGAGGC AGGAGACAAT
    GGCACAAAGG GATGGGAGGC TGGGAAACCC ATTGAGAGGT TGACTATATC TCTACCTGCC
    CCTGCTCGGA GCAAGGAGGA AAGCAGGAGG AGAGAGCGGA AGCACAGGAA GTCCAGGCGG
    GACTGTAGTA AGAAGTCAAC TGACCGCAGG TCTCTGAAGG ACAAGGAGAG GCAAGTCCTA
    GGCAACCTAG ATCTCCAGAG CCAGTGCCGG GACACCTCAA ATTCTACACT TAAGCAGAGT
    TCAAGGAAGT CCGAGACTGG CAGAAGAGGG TGCGAGGAAG CGCATCACCA CCAACCTACC
    CCACCTACTC CTCCAACTTC CGCACCCCCT CCAACGTCCC ACGGCACAGC CAACTCTGAC
    CTTCTAAAGA TGAGACCCTT CACCGAAGGG CCGCCTAAAG AGCTCAAGAT CCGCCTCATA
    AAGGTTGAAA GCGGAGATCG GGAGACTTTT ATAGCCTCCG AGGTGGAAGA GCGAAGGTTG
    CAGGCCAGTG AGCTGACCAT TAATAACACT GCTGCGGAGA TTGTAAGGGC CACCAGGAGT
    ATGGTGCTAA AAGGGAAGTT CAAAGAGTCC TACCTGCTGC CCTCGATGTC CGTGAAGCCG
    CCCTTGGGGA CGGAGCAGAA CCTTCCACGA GAGAAGCTGA ATCCCCCAAC TCCCAGTATA
    TATCTGGAGA GCAAACGAGA TGCCTTCTCC CCTATCCTGC TTCAGTTCTG CACCGACCCC
    AAGAATCCCA TCACTGTTAT CCGCGGTTTG GCCGGGTCCC TGCGTCTCAA CCTCGGACTC
    TTCTCCACCA AAACGCTCGT TGACGCCAAC GGGGAGCACA TGGTGGAGGT GCGCACTCAG
    GTACAACAGC CGTCAGATGA GAACTGGGAC CCGTCGGGCA CTCGCCAGAT CTGGCACTGC
    GAGAGCAGCC GTTCCCACAC CACCATCGCC AAATATGCAC AGTATCAGGC CTCCTCGTTC
    CAGGAGTCCC TGCAGGAGGA TAAGGAAAGT GATGACGACT CCAATGAACC AGATAGTACC
    ACAGAAACCC CACCCCCCAG TAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753878.1
    CDS542..5761
    Translation

    Target ORF information:

    RefSeq Version XM_031898018.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGAAGA GACATTTCGG AGCTAAACGG
    TGTCTCCCGC AGCTGAAGCG CCCGGTGCCC CCCTCAGACT CTTCCGCACT CCAGCTGAGC
    CCACACAATC CCCATGGAGG GGAGGAGCAA CCGATGAAAC GGAGGAGGAG CCGGAGCCCC
    GAACAGCAAA GGAGGGGGAA CCCTGGCCTG CACTCTCCTC CAGCCTCAGG GGGTTCTGGG
    AGTTTGTACC ATCAGGCACC CGTCAAGCCC AGTGTTTGGA ATCCACTCAC CCATGTGCCC
    TGGGCTGAGC GCAGGGAGCG GCAGGAACCC AGACCCCCCC ACGCGCCGTC TTACCCACCA
    TATTGCCCTC CACGCCCTCT ACCGCCTCCA CCACCACTTC ACCAGCAGCC CCGCCCTCCA
    AGTGACCTTA GTGAGGCTCG GGGGCAGAGG TGTGCAGAGG CCGTCCCCCC AGCACCGCCC
    CCTCTCCACG TGCCTTACGA TCAGAGAAGA CCCCGCGCCA CCCCGCCGAC TACCCCCAGG
    ACCGATCTCA CCACTTCCTC TGATCGCTAC CAGAATTCCG CAGGCAGCCC GTCTAGAATC
    TGCCCTCCTC TTGTAGAGCA GCTGGACCCC CCACAAACTA TATGTCAACC CCAGCTACCA
    GCTGTCTCTC GACCTCCTCC TCCTCTGCAA CAGGGATGGC TGCCCCCCCC AGCCAAAGAG
    GAGATGAAGA AGGGAGACCC ACTAGAGGAC ATTCTGTATG GAGATGGACC TACAAAGGTT
    AAACAGGAAG GGTTTTACCC ATCCCGTGAC ACCCAAAGAG AGGGCAATGT CTACAACCAC
    CAACCTCAGG GCATTACCAC CAGCAAAGCC AGGTCTGAGG CCTACCAGCC TGGAAGCCTG
    GATCACGTAC TGCAGAAGAT GGATGAATCT GGGTTAATTG CCCCAAAGAA GGAGGAAGAG
    ATTAAACAGG AAGTTAGAAA TCATCCATTG ACCCCAGAAC TGGAGGTTGG AGGCAAACAA
    TCAAGCCATG GACTCTCTAG AGCTGAGGGC AGCTATTCTG GTGGGAAGGG GTCACCAGAA
    GGCGCGGATG AAACGTCTTT CTGCCCCTCT TTGGCCCCCC ACCACAAAGA TACCTCAGGG
    AGCCTCCCGG TTCCAGGCTC AGTTGCATTA CCAGCCAAGC CAATGCCTAA CTTGACTCGC
    AGGCCACTAA GTCCGAACTC GGCCTTCTCG GAGAGAACGT CGGTGTGCGG ACAGGAGAAG
    GAGCAACCCA CTGGATGCAA CCAGCTCTTT GAATTCCCCG GGGGGCAGTT GCACGAGCCC
    TTTGAGGAAG CCGCGGAGTT TGCAAAAATA TTACCGGACG GGCTTGACGA CATCATGAAA
    ATGCTTGACG AGTCGATAGA AAAGGAAGAG GAAGGGGTTG GGAACGCCAG TGGGATGGAC
    GATAGTTCCT CCACTACATC CAGCAGTCCA CCAGCAGAGA CCATGCCAGC ATCCAGCCTA
    GTGAGCTCAA ATCAGACCAC TGTTGTGAAA AGCCCACCGC CCAATGCCAA AACAGCGGAA
    GTCCGGCCAA GTGACACCTT CCCCTATCAT GGCCCTAAGA AAGCCATGCT GGAGCAAGAA
    GCAAGCAAGG AGGCAAAGAC TGCGCAGCAC AGGGGAGGCA GCGTTCTGAA ATCACTCGCA
    AATGTTCTAG AGGGGCAGAA GTATTGCTAC CGAGGCAGCT CTGGGGCGAA GTCCGCAGGA
    AACCCGGCAT ACTTCACTGG TGCTATGGTG AGCAAGCGTA ATGTTGACCC ATACGCTACG
    CAATCTACCT CACACCGGGC CTGGGACACC AAGCCAGGGA CGCATGTTGG GGCAGCCCCT
    ACCTCAGAGG CTCTTAAGCC AGGGGTGATT GAGAAAAGGC CAGAGGTACG GACAGAGACG
    GACATTCTGG AGGACATAGC GAGGGCTTGT GAGACTTTGG TTGAGGGTGC AAAGGGGTCG
    GCGGCAAGCA GGACGGGAAA CGAGAGAGCA GTTGAGAAAG AGCCCAAAAA GTCTGAAGGG
    ATTGTGGACA GGAAGCCAGA TTTACAGTGG GGCATAGAAA AACGTGACAT CCATTGGGGG
    GCAAATAAAC TAGAAGGACC AGTTAAGCAG AAAGTAGATT TCAAGTGGAG AAGCGATACC
    CAGAAAGAAG TGGTTGAGAA GAAGCCAGAG GCTCCCAGGG CTACTGGCCA ACAGGACTAT
    GTTGAGAAGA AACATGGGGC AGACAAACCC CGCGTCCCCG TTGACAGGAA GCCTGAGACT
    TTGCAGAAGG CAGATGTACA GGAAGGAGTT GCTGAAATAA AGCAGGAAAC ATCTTATGGC
    ACAATCAGGC ATGAAGTGGC AGAGAGGCTG GAGGCGGCTC TTGAGAGAGA ATCCAAGAAG
    TCGGTGAAGG GAGCAGAGAA GAAATGTCTC CCAGGTCCAG CTGACTCCCC CTCAGGTACA
    GCCCGGGAAG ATCCCTCTAA AATACACGGT GCTAATGTCA AGGAGGCTGT AAAGGAGGCG
    ACAGCTAAGC GGCTGCAAGG GGTAAATGAC AGAGAGTCAC CAAAAAAGAA CACCGATTCT
    TTGGCCCAGG GAGGGGTCAG TAAGAACGTG GCGGCTGAAG TGGCAAAACC GAATGCCGAT
    AGCAAGGACA CTAAGTCATC TGAGGAAGCA CCGATGCCAA CAATGGAGGC AGGAGACAAT
    GGCACAAAGG GATGGGAGGC TGGGAAACCC ATTGAGAGGT TGACTATATC TCTACCTGCC
    CCTGCTCGGA GCAAGGAGGA AAGCAGGAGG AGAGAGCGGA AGCACAGGAA GTCCAGGCGG
    GACTGTAGTA AGAAGTCAAC TGACCGCAGG TCTCTGAAGG ACAAGGAGAG GCAAGTCCTA
    GGCAACCTAG ATCTCCAGAG CCAGTGCCGG GACACCTCAA ATTCTACACT TAAGCAGAGT
    TCAAGGAAGT CCGAGACTGG CAGAAGAGGG TGCGAGGAAG CGCATCACCA CCAACCTACC
    CCACCTACTC CTCCAACTTC CGCACCCCCT CCAACGTCCC ACGGCACAGC CAACTCTGAC
    CTTCTAAAGA TGAGACCCTT CACCGAAGGG CCGCCTAAAG AGCTCAAGAT CCGCCTCATA
    AAGGTTGAAA GCGGAGATCG GGAGACTTTT ATAGCCTCCG AGGTGGAAGA GCGAAGGTTG
    CAGGCCAGTG AGCTGACCAT TAATAACACT GCTGCGGAGA TTGTAAGGGC CACCAGGAGT
    ATGGTGCTAA AAGGGAAGTT CAAAGAGTCC TACCTGCTGC CCTCGATGTC CGTGAAGCCG
    CCCTTGGGGA CGGAGCAGAA CCTTCCACGA GAGAAGCTGA ATCCCCCAAC TCCCAGTATA
    TATCTGGAGA GCAAACGAGA TGCCTTCTCC CCTATCCTGC TTCAGTTCTG CACCGACCCC
    AAGAATCCCA TCACTGTTAT CCGCGGTTTG GCCGGGTCCC TGCGTCTCAA CCTCGGACTC
    TTCTCCACCA AAACGCTCGT TGACGCCAAC GGGGAGCACA TGGTGGAGGT GCGCACTCAG
    GTACAACAGC CGTCAGATGA GAACTGGGAC CCGTCGGGCA CTCGCCAGAT CTGGCACTGC
    GAGAGCAGCC GTTCCCACAC CACCATCGCC AAATATGCAC AGTATCAGGC CTCCTCGTTC
    CAGGAGTCCC TGCAGGAGGA TAAGGAAAGT GATGACGACT CCAATGAACC AGATAGTACC
    ACAGAAACCC CACCCCCCAG TAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53614
    Clone ID Related Accession (Same CDS sequence) XM_031898019.1 , XM_031898020.1
    Accession Version XM_031898019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753879.1
    CDS542..5761
    Translation

    Target ORF information:

    RefSeq Version XM_031898019.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53614
    Clone ID Related Accession (Same CDS sequence) XM_031898019.1 , XM_031898020.1
    Accession Version XM_031898020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753880.1
    CDS621..5840
    Translation

    Target ORF information:

    RefSeq Version XM_031898020.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53615
    Clone ID Related Accession (Same CDS sequence) XM_031898021.1
    Accession Version XM_031898021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGG AACCCAGACC CCCCCACGCG CCGTCTTACC CACCATATTG CCCTCCACGC
    CCTCTACCGC CTCCACCACC ACTTCACCAG CAGCCCCGCC CTCCAAGTGA CCTTAGTGAG
    GCTCGGGGGC AGAGGTGTGC AGAGGCCGTC CCCCCAGCAC CGCCCCCTCT CCACGTGCCT
    TACGATCAGA GAAGACCCCG CGCCACCCCG CCGACTACCC CCAGGACCGA TCTCACCACT
    TCCTCTGATC GCTACCAGAA TTCCGCAGGC AGCCCGTCTA GAATCTGCCC TCCTCTTGTA
    GAGCAGCTGG ACCCCCCACA AACTATATGT CAACCCCAGC TACCAGCTGT CTCTCGACCT
    CCTCCTCCTC TGCAACAGGG ATGGCTGCCC CCCCCAGCCA AAGAGGAGAT GAAGAAGGGA
    GACCCACTAG AGGACATTCT GTATGGAGAT GGACCTACAA AGGTTAAACA GGAAGGGTTT
    TACCCATCCC GTGACACCCA AAGAGAGGGC AATGTCTACA ACCACCAACC TCAGGGCATT
    ACCACCAGCA AAGCCAGGTC TGAGGCCTAC CAGCCTGGAA GCCTGGATCA CGTACTGCAG
    AAGATGGATG AATCTGGGTT AATTGCCCCA AAGAAGGAGG AAGAGATTAA ACAGGAAGTT
    AGAAATCATC CATTGACCCC AGAACTGGAG GTTGGAGGCA AACAATCAAG CCATGGACTC
    TCTAGAGCTG AGGGCAGCTA TTCTGGTGGG AAGGGGTCAC CAGAAGGCGC GGATGAAACG
    TCTTTCTGCC CCTCTTTGGC CCCCCACCAC AAAGATACCT CAGGGAGCCT CCCGGTTCCA
    GGCTCAGTTG CATTACCAGC CAAGCCAATG CCTAACTTGA CTCGCAGGCC ACTAAGTCCG
    AACTCGGCCT TCTCGGAGAG AACGTCGGTG TGCGGACAGG AGAAGGAGCA ACCCACTGGA
    TGCAACCAGC TCTTTGAATT CCCCGGGGGG CAGTTGCACG AGCCCTTTGA GGAAGCCGCG
    GAGTTTGCAA AAATATTACC GGACGGGCTT GACGACATCA TGAAAATGCT TGACGAGTCG
    ATAGAAAAGG AAGAGGAAGG GGTTGGGAAC GCCAGTGGGA TGGACGATAG TTCCTCCACT
    ACATCCAGCA GTCCACCAGC AGAGACCATG CCAGCATCCA GCCTAGTGAG CTCAAATCAG
    ACCACTGTTG TGAAAAGCCC ACCGCCCAAT GCCAAAACAG CGGAAGTCCG GCCAAGTGAC
    ACCTTCCCCT ATCATGGCCC TAAGAAAGCC ATGCTGGAGC AAGAAGCAAG CAAGGAGGCA
    AAGACTGCGC AGCACAGGGG AGGCAGCGTT CTGAAATCAC TCGCAAATGT TCTAGAGGGG
    CAGAAGTATT GCTACCGAGG CAGCTCTGGG GCGAAGTCCG CAGGAAACCC GGCATACTTC
    ACTGGTGCTA TGGTGAGCAA GCGTAATGTT GACCCATACG CTACGCAATC TACCTCACAC
    CGGGCCTGGG ACACCAAGCC AGGGACGCAT GTTGGGGCAG CCCCTACCTC AGAGGCTCTT
    AAGCCAGGGG TGATTGAGAA AAGGCCAGAG GTACGGACAG AGACGGACAT TCTGGAGGAC
    ATAGCGAGGG CTTGTGAGAC TTTGGTTGAG GGTGCAAAGG GGTCGGCGGC AAGCAGGACG
    GGAAACGAGA GAGCAGTTGA GAAAGAGCCC AAAAAGTCTG AAGGGATTGT GGACAGGAAG
    CCAGATTTAC AGTGGGGCAT AGAAAAACGT GACATCCATT GGGGGGCAAA TAAACTAGAA
    GGACCAGTTA AGCAGAAAGT AGATTTCAAG TGGAGAAGCG ATACCCAGAA AGAAGTGGTT
    GAGAAGAAGC CAGAGGCTCC CAGGGCTACT GGCCAACAGG ACTATGTTGA GAAGAAACAT
    GGGGCAGACA AACCCCGCGT CCCCGTTGAC AGGAAGCCTG AGACTTTGCA GAAGGCAGAT
    GTACAGGAAG GAGTTGCTGA AATAAAGCAG GAAACATCTT ATGGCACAAT CAGGCATGAA
    GTGGCAGAGA GGCTGGAGGC GGCTCTTGAG AGAGAATCCA AGAAGTCGGT GAAGGGAGCA
    GAGAAGAAAT GTCTCCCAGG TCCAGCTGAC TCCCCCTCAG GTACAGCCCG GGAAGATCCC
    TCTAAAATAC ACGGTGCTAA TGTCAAGGAG GCTGTAAAGG AGGCGACAGC TAAGCGGCTG
    CAAGGGGTAA ATGACAGAGA GTCACCAAAA AAGAACACCG ATTCTTTGGC CCAGGGAGGG
    GTCAGTAAGA ACGTGGCGGC TGAAGTGGCA AAACCGAATG CCGATAGCAA GGACACTAAG
    TCATCTGAGG AAGCACCGAT GCCAACAATG GAGGCAGGAG ACAATGGCAC AAAGGGATGG
    GAGGCTGGGA AACCCATTGA GAGGTTGACT ATATCTCTAC CTGCCCCTGC TCGGAGCAAG
    GAGGAAAGCA GGAGGAGAGA GCGGAAGCAC AGGAAGTCCA GGCGGGACTG TAGTAAGAAG
    TCAACTGACC GCAGGTCTCT GAAGGACAAG GAGAGGCAAG TCCTAGGCAA CCTAGATCTC
    CAGAGCCAGT GCCGGGACAC CTCAAATTCT ACACTTAAGC AGAGTTCAAG GAAGTCCGAG
    ACTGGCAGAA GAGGGTGCGA GGAAGCGCAT CACCACCAAC CTACCCCACC TACTCCTCCA
    ACTTCCGCAC CCCCTCCAAC GTCCCACGGC ACAGCCAACT CTGACCTTCT AAAGATGAGA
    CCCTTCACCG AAGGGCCGCC TAAAGAGCTC AAGATCCGCC TCATAAAGGT TGAAAGCGGA
    GATCGGGAGA CTTTTATAGC CTCCGAGGTG GAAGAGCGAA GGTTGCAGGC CAGTGAGCTG
    ACCATTAATA ACACTGCTGC GGAGATTGTA AGGGCCACCA GGAGTATGGT GCTAAAAGGG
    AAGTTCAAAG AGTCCTACCT GCTGCCCTCG ATGTCCGTGA AGCCGCCCTT GGGGACGGAG
    CAGAACCTTC CACGAGAGAA GCTGAATCCC CCAACTCCCA GTATATATCT GGAGAGCAAA
    CGAGATGCCT TCTCCCCTAT CCTGCTTCAG TTCTGCACCG ACCCCAAGAA TCCCATCACT
    GTTATCCGCG GTTTGGCCGG GTCCCTGCGT CTCAACCTCG GACTCTTCTC CACCAAAACG
    CTCGTTGACG CCAACGGGGA GCACATGGTG GAGGTGCGCA CTCAGGTACA ACAGCCGTCA
    GATGAGAACT GGGACCCGTC GGGCACTCGC CAGATCTGGC ACTGCGAGAG CAGCCGTTCC
    CACACCACCA TCGCCAAATA TGCACAGTAT CAGGCCTCCT CGTTCCAGGA GTCCCTGCAG
    GAGGATAAGG AAAGTGATGA CGACTCCAAT GAACCAGATA GTACCACAGA AACCCCACCC
    CCCAGTAGCA CAAACCCGGA GCACAAGACC CCACAGATTA TCAAGTTCGG CACCAATATT
    GATCTGTCTG ATCCCAAAAG GTGGAAACCT CAGTTACAGG AGCTGCAGAA GTTGCCATCT
    TTCATGCGTG TTACATCCAA TGCCAACATG CTAAGCCACG TGGGCCATAC TATACTTGGC
    ATGAACACCG TTCAGCTCTA CATGAAGGTT CCGGGCAGCA GGACCCCGGG TCACCAGGAG
    AATAACAACT TCTGCTCTGT GAATATAAAC ATCGGCCCCG GGGACTGCGA ATGGTTCGCT
    GTACATGAAA ATTACTGGGA GAAAATCAGC GCCTTCTGTG ACAAACACAA TCTGGACTAC
    CTGACCGGAT CTTGGTGGCC GGTTCTAGAA GACCTGTACC GCGCCGACAT CCCAGTTTAT
    CGGTTTGTTC AGCGCCCGGG AGACCTGGTC TGGATCAACG CCGGAACTGT GCATTGGGTG
    CAGGCTACAG GCTGGTGCAA CAACATCGCG TGGAACGTGG GGCCCCTGAC ACCCTATCAG
    TACCAACTGG CGCTGGAGCG ATTTGAGTGG AACGAAGTCA AGAACGTGAA ATCCATCGTC
    CCAATGATCC ACGTGTCCTG GAACGTGGCC CGGACCGTCA AAATCAGTGA TCCAGACCTG
    TATCGCATGA TCAAGTACTG CCTGATGCAG TCTATGAAGC GCTGCGTTTT GCAGAGGGAC
    AGCCTGATCC GCGGCGGGAA GAAAATCGCC TACCAGAGCA GAGTGAAAGA TGAACCGGCC
    TATTACTGCA ACGAGTGTGA TGTGGAGGTG TTTAACATCC TATTCGTGAC CAGCGAGAGC
    GGTGGGAAGA ACACGTACCT GGTGCACTGT GAGAGCTGCT CCCGGAGCGC CAGTAGCGCA
    AGCAGCGCCA GCAGCAACGT CGTCATCTTG GAGCAGTTTA AGACCGAGGA GCTAATGCAC
    ATATACGACA ACTTCCACCT GGCTCCACCC CCGAACATCC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753881.1
    CDS542..5626
    Translation

    Target ORF information:

    RefSeq Version XM_031898021.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGG AACCCAGACC CCCCCACGCG CCGTCTTACC CACCATATTG CCCTCCACGC
    CCTCTACCGC CTCCACCACC ACTTCACCAG CAGCCCCGCC CTCCAAGTGA CCTTAGTGAG
    GCTCGGGGGC AGAGGTGTGC AGAGGCCGTC CCCCCAGCAC CGCCCCCTCT CCACGTGCCT
    TACGATCAGA GAAGACCCCG CGCCACCCCG CCGACTACCC CCAGGACCGA TCTCACCACT
    TCCTCTGATC GCTACCAGAA TTCCGCAGGC AGCCCGTCTA GAATCTGCCC TCCTCTTGTA
    GAGCAGCTGG ACCCCCCACA AACTATATGT CAACCCCAGC TACCAGCTGT CTCTCGACCT
    CCTCCTCCTC TGCAACAGGG ATGGCTGCCC CCCCCAGCCA AAGAGGAGAT GAAGAAGGGA
    GACCCACTAG AGGACATTCT GTATGGAGAT GGACCTACAA AGGTTAAACA GGAAGGGTTT
    TACCCATCCC GTGACACCCA AAGAGAGGGC AATGTCTACA ACCACCAACC TCAGGGCATT
    ACCACCAGCA AAGCCAGGTC TGAGGCCTAC CAGCCTGGAA GCCTGGATCA CGTACTGCAG
    AAGATGGATG AATCTGGGTT AATTGCCCCA AAGAAGGAGG AAGAGATTAA ACAGGAAGTT
    AGAAATCATC CATTGACCCC AGAACTGGAG GTTGGAGGCA AACAATCAAG CCATGGACTC
    TCTAGAGCTG AGGGCAGCTA TTCTGGTGGG AAGGGGTCAC CAGAAGGCGC GGATGAAACG
    TCTTTCTGCC CCTCTTTGGC CCCCCACCAC AAAGATACCT CAGGGAGCCT CCCGGTTCCA
    GGCTCAGTTG CATTACCAGC CAAGCCAATG CCTAACTTGA CTCGCAGGCC ACTAAGTCCG
    AACTCGGCCT TCTCGGAGAG AACGTCGGTG TGCGGACAGG AGAAGGAGCA ACCCACTGGA
    TGCAACCAGC TCTTTGAATT CCCCGGGGGG CAGTTGCACG AGCCCTTTGA GGAAGCCGCG
    GAGTTTGCAA AAATATTACC GGACGGGCTT GACGACATCA TGAAAATGCT TGACGAGTCG
    ATAGAAAAGG AAGAGGAAGG GGTTGGGAAC GCCAGTGGGA TGGACGATAG TTCCTCCACT
    ACATCCAGCA GTCCACCAGC AGAGACCATG CCAGCATCCA GCCTAGTGAG CTCAAATCAG
    ACCACTGTTG TGAAAAGCCC ACCGCCCAAT GCCAAAACAG CGGAAGTCCG GCCAAGTGAC
    ACCTTCCCCT ATCATGGCCC TAAGAAAGCC ATGCTGGAGC AAGAAGCAAG CAAGGAGGCA
    AAGACTGCGC AGCACAGGGG AGGCAGCGTT CTGAAATCAC TCGCAAATGT TCTAGAGGGG
    CAGAAGTATT GCTACCGAGG CAGCTCTGGG GCGAAGTCCG CAGGAAACCC GGCATACTTC
    ACTGGTGCTA TGGTGAGCAA GCGTAATGTT GACCCATACG CTACGCAATC TACCTCACAC
    CGGGCCTGGG ACACCAAGCC AGGGACGCAT GTTGGGGCAG CCCCTACCTC AGAGGCTCTT
    AAGCCAGGGG TGATTGAGAA AAGGCCAGAG GTACGGACAG AGACGGACAT TCTGGAGGAC
    ATAGCGAGGG CTTGTGAGAC TTTGGTTGAG GGTGCAAAGG GGTCGGCGGC AAGCAGGACG
    GGAAACGAGA GAGCAGTTGA GAAAGAGCCC AAAAAGTCTG AAGGGATTGT GGACAGGAAG
    CCAGATTTAC AGTGGGGCAT AGAAAAACGT GACATCCATT GGGGGGCAAA TAAACTAGAA
    GGACCAGTTA AGCAGAAAGT AGATTTCAAG TGGAGAAGCG ATACCCAGAA AGAAGTGGTT
    GAGAAGAAGC CAGAGGCTCC CAGGGCTACT GGCCAACAGG ACTATGTTGA GAAGAAACAT
    GGGGCAGACA AACCCCGCGT CCCCGTTGAC AGGAAGCCTG AGACTTTGCA GAAGGCAGAT
    GTACAGGAAG GAGTTGCTGA AATAAAGCAG GAAACATCTT ATGGCACAAT CAGGCATGAA
    GTGGCAGAGA GGCTGGAGGC GGCTCTTGAG AGAGAATCCA AGAAGTCGGT GAAGGGAGCA
    GAGAAGAAAT GTCTCCCAGG TCCAGCTGAC TCCCCCTCAG GTACAGCCCG GGAAGATCCC
    TCTAAAATAC ACGGTGCTAA TGTCAAGGAG GCTGTAAAGG AGGCGACAGC TAAGCGGCTG
    CAAGGGGTAA ATGACAGAGA GTCACCAAAA AAGAACACCG ATTCTTTGGC CCAGGGAGGG
    GTCAGTAAGA ACGTGGCGGC TGAAGTGGCA AAACCGAATG CCGATAGCAA GGACACTAAG
    TCATCTGAGG AAGCACCGAT GCCAACAATG GAGGCAGGAG ACAATGGCAC AAAGGGATGG
    GAGGCTGGGA AACCCATTGA GAGGTTGACT ATATCTCTAC CTGCCCCTGC TCGGAGCAAG
    GAGGAAAGCA GGAGGAGAGA GCGGAAGCAC AGGAAGTCCA GGCGGGACTG TAGTAAGAAG
    TCAACTGACC GCAGGTCTCT GAAGGACAAG GAGAGGCAAG TCCTAGGCAA CCTAGATCTC
    CAGAGCCAGT GCCGGGACAC CTCAAATTCT ACACTTAAGC AGAGTTCAAG GAAGTCCGAG
    ACTGGCAGAA GAGGGTGCGA GGAAGCGCAT CACCACCAAC CTACCCCACC TACTCCTCCA
    ACTTCCGCAC CCCCTCCAAC GTCCCACGGC ACAGCCAACT CTGACCTTCT AAAGATGAGA
    CCCTTCACCG AAGGGCCGCC TAAAGAGCTC AAGATCCGCC TCATAAAGGT TGAAAGCGGA
    GATCGGGAGA CTTTTATAGC CTCCGAGGTG GAAGAGCGAA GGTTGCAGGC CAGTGAGCTG
    ACCATTAATA ACACTGCTGC GGAGATTGTA AGGGCCACCA GGAGTATGGT GCTAAAAGGG
    AAGTTCAAAG AGTCCTACCT GCTGCCCTCG ATGTCCGTGA AGCCGCCCTT GGGGACGGAG
    CAGAACCTTC CACGAGAGAA GCTGAATCCC CCAACTCCCA GTATATATCT GGAGAGCAAA
    CGAGATGCCT TCTCCCCTAT CCTGCTTCAG TTCTGCACCG ACCCCAAGAA TCCCATCACT
    GTTATCCGCG GTTTGGCCGG GTCCCTGCGT CTCAACCTCG GACTCTTCTC CACCAAAACG
    CTCGTTGACG CCAACGGGGA GCACATGGTG GAGGTGCGCA CTCAGGTACA ACAGCCGTCA
    GATGAGAACT GGGACCCGTC GGGCACTCGC CAGATCTGGC ACTGCGAGAG CAGCCGTTCC
    CACACCACCA TCGCCAAATA TGCACAGTAT CAGGCCTCCT CGTTCCAGGA GTCCCTGCAG
    GAGGATAAGG AAAGTGATGA CGACTCCAAT GAACCAGATA GTACCACAGA AACCCCACCC
    CCCAGTAGCA CAAACCCGGA GCACAAGACC CCACAGATTA TCAAGTTCGG CACCAATATT
    GATCTGTCTG ATCCCAAAAG GTGGAAACCT CAGTTACAGG AGCTGCAGAA GTTGCCATCT
    TTCATGCGTG TTACATCCAA TGCCAACATG CTAAGCCACG TGGGCCATAC TATACTTGGC
    ATGAACACCG TTCAGCTCTA CATGAAGGTT CCGGGCAGCA GGACCCCGGG TCACCAGGAG
    AATAACAACT TCTGCTCTGT GAATATAAAC ATCGGCCCCG GGGACTGCGA ATGGTTCGCT
    GTACATGAAA ATTACTGGGA GAAAATCAGC GCCTTCTGTG ACAAACACAA TCTGGACTAC
    CTGACCGGAT CTTGGTGGCC GGTTCTAGAA GACCTGTACC GCGCCGACAT CCCAGTTTAT
    CGGTTTGTTC AGCGCCCGGG AGACCTGGTC TGGATCAACG CCGGAACTGT GCATTGGGTG
    CAGGCTACAG GCTGGTGCAA CAACATCGCG TGGAACGTGG GGCCCCTGAC ACCCTATCAG
    TACCAACTGG CGCTGGAGCG ATTTGAGTGG AACGAAGTCA AGAACGTGAA ATCCATCGTC
    CCAATGATCC ACGTGTCCTG GAACGTGGCC CGGACCGTCA AAATCAGTGA TCCAGACCTG
    TATCGCATGA TCAAGTACTG CCTGATGCAG TCTATGAAGC GCTGCGTTTT GCAGAGGGAC
    AGCCTGATCC GCGGCGGGAA GAAAATCGCC TACCAGAGCA GAGTGAAAGA TGAACCGGCC
    TATTACTGCA ACGAGTGTGA TGTGGAGGTG TTTAACATCC TATTCGTGAC CAGCGAGAGC
    GGTGGGAAGA ACACGTACCT GGTGCACTGT GAGAGCTGCT CCCGGAGCGC CAGTAGCGCA
    AGCAGCGCCA GCAGCAACGT CGTCATCTTG GAGCAGTTTA AGACCGAGGA GCTAATGCAC
    ATATACGACA ACTTCCACCT GGCTCCACCC CCGAACATCC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa20376
    Clone ID Related Accession (Same CDS sequence) NM_001278756.1
    Accession Version NM_001278756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product lysine-specific demethylase 6B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN107757.1. On or before Jul 15, 2013 this sequence version replaced XM_002943186.2, XM_004913200.1, XM_004913201.1, XM_004913202.1, XM_004913203.1, XM_004913204.1. ##Evidence-Data-START## Transcript exon combination :: JN107757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00861997 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATA AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ACGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA ACGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTTTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGACG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCCC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACCGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGTGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCAGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAT GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGTGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CGTCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TTGTCCCGAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGCGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTCAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265685.1
    CDS234..5453
    Translation

    Target ORF information:

    RefSeq Version NM_001278756.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
    GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT
    TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT
    GGGCATCCCA GCAAACCTTA TTACCCAATA AGCTCACCCC CCGGCCCCCG CATTGGGAAG
    TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ACGAGCAGCA GCCCTCCCTC
    TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC
    TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG
    CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC
    CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA
    CGGTGTCTCC CGCAGCTGAA ACGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG
    AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC
    CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT
    GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG
    CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA
    CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT
    CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG
    CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC
    AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA
    ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA
    CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA
    GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG
    GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC
    CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC
    CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA
    GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA
    CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA
    GAAGGCGCGG ATGAAACGTC TTTTTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA
    GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT
    CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG
    AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG
    CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG
    AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGACG
    GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC
    CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCCC CGCCCAATGC CAAAACAGCG
    GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA
    GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC
    GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA
    GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT
    ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC
    CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG
    ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG
    TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA
    GGGATTGTGG ACCGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG
    GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT
    ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC
    TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGTGTCC CCGTTGACAG GAAGCCTGAG
    ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT
    GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG
    AAGTCAGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT
    ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG
    GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT
    TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC
    GATAGCAAGG ACACTAAGTC ATCTGAGGAT GCACCGATGC CAACAATGGA GGCAGGAGAC
    AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT
    GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG
    CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC
    CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG
    AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT
    ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT
    GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC
    ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG
    TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG
    AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG
    CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT
    ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC
    CCCAAGAATC CCATCACTGT TATCCGTGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA
    CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT
    CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC
    TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG
    TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT
    ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG
    TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG
    CAGAAGTTGC CGTCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC
    CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC
    CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC
    TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA
    CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC
    GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA
    ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC
    CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC
    GTGAAATCCA TTGTCCCGAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC
    AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC
    GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG
    AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC
    GTGACCAGCG AGAGCGGCGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG
    AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTCAAGACC
    GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.