wrn gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018096746.1	XP_017952235.1	Werner syndrome ATP-dependent helicase isoform X5
XM_012954857.3	XP_012810311.2	Werner syndrome ATP-dependent helicase isoform X4
XM_012954853.2	XP_012810307.1	Werner syndrome ATP-dependent helicase isoform X2
XM_012954857.2	XP_012810311.1	Werner syndrome ATP-dependent helicase isoform X4
XM_031904344.1	XP_031760204.1	Werner syndrome ATP-dependent helicase isoform X1
XM_012954852.3	XP_012810306.2	Werner syndrome ATP-dependent helicase isoform X1
XM_012954853.3	XP_012810307.2	Werner syndrome ATP-dependent helicase isoform X2
XM_012954855.2	XP_012810309.1	Werner syndrome ATP-dependent helicase isoform X3
XM_012954852.2	XP_012810306.1	Werner syndrome ATP-dependent helicase isoform X1
XM_031904350.1	XP_031760210.1	Werner syndrome ATP-dependent helicase isoform X1
XM_012954855.3	XP_012810309.2	Werner syndrome ATP-dependent helicase isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42005	XM_018096746.1 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa48391	XM_012954852.3 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48391	XM_031904350.1 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48391	XM_031904344.1 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48392	XM_012954853.3 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48393	XM_012954855.3 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa48394	XM_012954857.3 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa28798	XM_012954852.2 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa28799	XM_012954853.2 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa28800	XM_012954855.2 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa28801	XM_012954857.2 Latest version!	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

wrn ( XM_018096746.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952235.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa42005
Clone ID Related Accession (Same CDS sequence)	XM_018096746.1
Accession Version	XM_018096746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAACTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952235.1
CDS	327..4547
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSREGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNESLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPRLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTTLYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_018096746.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096746.1

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAACTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_031904344.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760204.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa48391
Clone ID Related Accession (Same CDS sequence)	XM_031904344.1 , XM_012954852.3 , XM_031904350.1
Accession Version	XM_031904344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760204.1
CDS	168..4469
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRVGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_031904344.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904344.1

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954852.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810306.2 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.

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CloneID	OXa48391
Clone ID Related Accession (Same CDS sequence)	XM_031904344.1 , XM_012954852.3 , XM_031904350.1
Accession Version	XM_012954852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810306.2
CDS	299..4600
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRVGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954852.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954852.3

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_031904350.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760210.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.

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CloneID	OXa48391
Clone ID Related Accession (Same CDS sequence)	XM_031904344.1 , XM_012954852.3 , XM_031904350.1
Accession Version	XM_031904350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760210.1
CDS	205..4506
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRVGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_031904350.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904350.1

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAGCTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954853.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810307.2 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa48392
Clone ID Related Accession (Same CDS sequence)	XM_012954853.3
Accession Version	XM_012954853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCTTGAAACT TCAGACACCA 
TGTAAACCTT TGCAAGCGGC TGAACCAGAT GTTTCACCCA AAGAGATGGA ACTTCAGACA 
GCTCTATATG GCAGATTGGT AGTAGCTAGA CAAAAGATTG CTAGCGAGAG AGATATACCG 
CCTGCTGTCC TAGCTACCAA CAAAGTATTA GTAGACATGG CAAAATTGAG GCCAACCACA 
ATCGAAAACA TGAAGAAACT AGACGGAGTG TCAGAAGCCA AGTCAGCAAT GCTTGCCCCC 
CTAGTGGAAG TTGTTAAAGA GTTCTGCATT GCTAATAGTT TGAAGATGGA TATATTCTCA 
AGATCTGGAT CCCAATCTGA GTTGACATCT TCAACTCAAA GGGAGCAAGA CAGCACTACT 
TTGCCAGAGT CACAGCGTAT GTCCTATTCA TTGTTTCAGG AGCAGAATTT GTCTTTGAAA 
AAAGTTGCAG ATGCTCGCTG TTTAAGCATG GCAGCAGTCG GTATGCATTT GTGGCAAGCC 
TTGAAAGCTG GTTATAGTGT CGATGTTCAG AGAGCTGGCC TGACTCCTGG AATTAGGAAG 
CTAATTATAG ATAATATTAA AAATCCTCCC ATTAATTCAG ACCTTTCAAG TTTTAAAGCT 
ATTCGTGAAT CCATTCCTGC TAACATTGAC AGCTACCTCA TTAGAATGGC GATTGCTCTG 
CTTGAGAAGG AGGAGAGCAA TGGGGCCCAA GGACAACTTG ACTTGCCAAC ACAGAAAACA 
CTAGTTAAGC TAGAAGAGCA GCCCAAAAAT ATTTCAGTTG AGAATATAAA GCACAAAGCA 
ACTATGAACA ATTCATTGTG GATTGAAAAA AAGCCCACAC AGCCCACAAC TGCAGAACTA 
GAAGAAACAA AAGCAAAACC GTTGGCCCCC ATCATGCTGG CATCATGGAA TGAACCCAGT 
CTGGATGCTG ACACAGAGGA ATTATTTTCT GAATCACAGA GTTACACTAC AACCCCAAAA 
AGAAAACTTC CTCAATGGTT CGGTCCTGCA AAAGGAAATG CGGGAACAAA TGCTGTCAAA 
AAAGCAAAAA CTGGGAAGAA AAAAGGCATT TTTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810307.2
CDS	299..4597
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRVGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954853.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954853.3

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGT GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTACA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGCCTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCTTGAAACT TCAGACACCA 
TGTAAACCTT TGCAAGCGGC TGAACCAGAT GTTTCACCCA AAGAGATGGA ACTTCAGACA 
GCTCTATATG GCAGATTGGT AGTAGCTAGA CAAAAGATTG CTAGCGAGAG AGATATACCG 
CCTGCTGTCC TAGCTACCAA CAAAGTATTA GTAGACATGG CAAAATTGAG GCCAACCACA 
ATCGAAAACA TGAAGAAACT AGACGGAGTG TCAGAAGCCA AGTCAGCAAT GCTTGCCCCC 
CTAGTGGAAG TTGTTAAAGA GTTCTGCATT GCTAATAGTT TGAAGATGGA TATATTCTCA 
AGATCTGGAT CCCAATCTGA GTTGACATCT TCAACTCAAA GGGAGCAAGA CAGCACTACT 
TTGCCAGAGT CACAGCGTAT GTCCTATTCA TTGTTTCAGG AGCAGAATTT GTCTTTGAAA 
AAAGTTGCAG ATGCTCGCTG TTTAAGCATG GCAGCAGTCG GTATGCATTT GTGGCAAGCC 
TTGAAAGCTG GTTATAGTGT CGATGTTCAG AGAGCTGGCC TGACTCCTGG AATTAGGAAG 
CTAATTATAG ATAATATTAA AAATCCTCCC ATTAATTCAG ACCTTTCAAG TTTTAAAGCT 
ATTCGTGAAT CCATTCCTGC TAACATTGAC AGCTACCTCA TTAGAATGGC GATTGCTCTG 
CTTGAGAAGG AGGAGAGCAA TGGGGCCCAA GGACAACTTG ACTTGCCAAC ACAGAAAACA 
CTAGTTAAGC TAGAAGAGCA GCCCAAAAAT ATTTCAGTTG AGAATATAAA GCACAAAGCA 
ACTATGAACA ATTCATTGTG GATTGAAAAA AAGCCCACAC AGCCCACAAC TGCAGAACTA 
GAAGAAACAA AAGCAAAACC GTTGGCCCCC ATCATGCTGG CATCATGGAA TGAACCCAGT 
CTGGATGCTG ACACAGAGGA ATTATTTTCT GAATCACAGA GTTACACTAC AACCCCAAAA 
AGAAAACTTC CTCAATGGTT CGGTCCTGCA AAAGGAAATG CGGGAACAAA TGCTGTCAAA 
AAAGCAAAAA CTGGGAAGAA AAAAGGCATT TTTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954855.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810309.2 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa48393
Clone ID Related Accession (Same CDS sequence)	XM_012954855.3
Accession Version	XM_012954855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTACAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GCCTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAGCTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810309.2
CDS	299..4579
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954855.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954855.3

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTACAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GCCTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAGCTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954857.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810311.2 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X6, mRNA.

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CloneID	OXa48394
Clone ID Related Accession (Same CDS sequence)	XM_012954857.3
Accession Version	XM_012954857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTACAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GCCTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CTTGAAACTT CAGACACCAT GTAAACCTTT GCAAGCGGCT 
GAACCAGATG TTTCACCCAA AGAGATGGAA CTTCAGACAG CTCTATATGG CAGATTGGTA 
GTAGCTAGAC AAAAGATTGC TAGCGAGAGA GATATACCGC CTGCTGTCCT AGCTACCAAC 
AAAGTATTAG TAGACATGGC AAAATTGAGG CCAACCACAA TCGAAAACAT GAAGAAACTA 
GACGGAGTGT CAGAAGCCAA GTCAGCAATG CTTGCCCCCC TAGTGGAAGT TGTTAAAGAG 
TTCTGCATTG CTAATAGTTT GAAGATGGAT ATATTCTCAA GATCTGGATC CCAATCTGAG 
TTGACATCTT CAACTCAAAG GGAGCAAGAC AGCACTACTT TGCCAGAGTC ACAGCGTATG 
TCCTATTCAT TGTTTCAGGA GCAGAATTTG TCTTTGAAAA AAGTTGCAGA TGCTCGCTGT 
TTAAGCATGG CAGCAGTCGG TATGCATTTG TGGCAAGCCT TGAAAGCTGG TTATAGTGTC 
GATGTTCAGA GAGCTGGCCT GACTCCTGGA ATTAGGAAGC TAATTATAGA TAATATTAAA 
AATCCTCCCA TTAATTCAGA CCTTTCAAGT TTTAAAGCTA TTCGTGAATC CATTCCTGCT 
AACATTGACA GCTACCTCAT TAGAATGGCG ATTGCTCTGC TTGAGAAGGA GGAGAGCAAT 
GGGGCCCAAG GACAACTTGA CTTGCCAACA CAGAAAACAC TAGTTAAGCT AGAAGAGCAG 
CCCAAAAATA TTTCAGTTGA GAATATAAAG CACAAAGCAA CTATGAACAA TTCATTGTGG 
ATTGAAAAAA AGCCCACACA GCCCACAACT GCAGAACTAG AAGAAACAAA AGCAAAACCG 
TTGGCCCCCA TCATGCTGGC ATCATGGAAT GAACCCAGTC TGGATGCTGA CACAGAGGAA 
TTATTTTCTG AATCACAGAG TTACACTACA ACCCCAAAAA GAAAACTTCC TCAATGGTTC 
GGTCCTGCAA AAGGAAATGC GGGAACAAAT GCTGTCAAAA AAGCAAAAAC TGGGAAGAAA 
AAAGGCATTT TTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810311.2
CDS	299..4576
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPSLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSLKLQTPCKPLQAAEPDVSPKEMELQTALYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954857.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954857.3

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTACAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GCCTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CTTGAAACTT CAGACACCAT GTAAACCTTT GCAAGCGGCT 
GAACCAGATG TTTCACCCAA AGAGATGGAA CTTCAGACAG CTCTATATGG CAGATTGGTA 
GTAGCTAGAC AAAAGATTGC TAGCGAGAGA GATATACCGC CTGCTGTCCT AGCTACCAAC 
AAAGTATTAG TAGACATGGC AAAATTGAGG CCAACCACAA TCGAAAACAT GAAGAAACTA 
GACGGAGTGT CAGAAGCCAA GTCAGCAATG CTTGCCCCCC TAGTGGAAGT TGTTAAAGAG 
TTCTGCATTG CTAATAGTTT GAAGATGGAT ATATTCTCAA GATCTGGATC CCAATCTGAG 
TTGACATCTT CAACTCAAAG GGAGCAAGAC AGCACTACTT TGCCAGAGTC ACAGCGTATG 
TCCTATTCAT TGTTTCAGGA GCAGAATTTG TCTTTGAAAA AAGTTGCAGA TGCTCGCTGT 
TTAAGCATGG CAGCAGTCGG TATGCATTTG TGGCAAGCCT TGAAAGCTGG TTATAGTGTC 
GATGTTCAGA GAGCTGGCCT GACTCCTGGA ATTAGGAAGC TAATTATAGA TAATATTAAA 
AATCCTCCCA TTAATTCAGA CCTTTCAAGT TTTAAAGCTA TTCGTGAATC CATTCCTGCT 
AACATTGACA GCTACCTCAT TAGAATGGCG ATTGCTCTGC TTGAGAAGGA GGAGAGCAAT 
GGGGCCCAAG GACAACTTGA CTTGCCAACA CAGAAAACAC TAGTTAAGCT AGAAGAGCAG 
CCCAAAAATA TTTCAGTTGA GAATATAAAG CACAAAGCAA CTATGAACAA TTCATTGTGG 
ATTGAAAAAA AGCCCACACA GCCCACAACT GCAGAACTAG AAGAAACAAA AGCAAAACCG 
TTGGCCCCCA TCATGCTGGC ATCATGGAAT GAACCCAGTC TGGATGCTGA CACAGAGGAA 
TTATTTTCTG AATCACAGAG TTACACTACA ACCCCAAAAA GAAAACTTCC TCAATGGTTC 
GGTCCTGCAA AAGGAAATGC GGGAACAAAT GCTGTCAAAA AAGCAAAAAC TGGGAAGAAA 
AAAGGCATTT TTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954852.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810306.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.

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CloneID	OXa28798
Clone ID Related Accession (Same CDS sequence)	XM_012954852.2
Accession Version	XM_012954852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTGCA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGACTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAACTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810306.1
CDS	327..4628
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSREGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPRLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTTLYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954852.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954852.2

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTGCA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGACTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCAGCTTGAA ACTTCAGACA 
CCATGTAAAC CTTTGCAAGC GGCTGAACCA GATGTTTCAC CCAAAGAGAT GGAACTTCAG 
ACAACTCTAT ATGGCAGATT GGTAGTAGCT AGACAAAAGA TTGCTAGCGA GAGAGATATA 
CCGCCTGCTG TCCTAGCTAC CAACAAAGTA TTAGTAGACA TGGCAAAATT GAGGCCAACC 
ACAATCGAAA ACATGAAGAA ACTAGACGGA GTGTCAGAAG CCAAGTCAGC AATGCTTGCC 
CCCCTAGTGG AAGTTGTTAA AGAGTTCTGC ATTGCTAATA GTTTGAAGAT GGATATATTC 
TCAAGATCTG GATCCCAATC TGAGTTGACA TCTTCAACTC AAAGGGAGCA AGACAGCACT 
ACTTTGCCAG AGTCACAGCG TATGTCCTAT TCATTGTTTC AGGAGCAGAA TTTGTCTTTG 
AAAAAAGTTG CAGATGCTCG CTGTTTAAGC ATGGCAGCAG TCGGTATGCA TTTGTGGCAA 
GCCTTGAAAG CTGGTTATAG TGTCGATGTT CAGAGAGCTG GCCTGACTCC TGGAATTAGG 
AAGCTAATTA TAGATAATAT TAAAAATCCT CCCATTAATT CAGACCTTTC AAGTTTTAAA 
GCTATTCGTG AATCCATTCC TGCTAACATT GACAGCTACC TCATTAGAAT GGCGATTGCT 
CTGCTTGAGA AGGAGGAGAG CAATGGGGCC CAAGGACAAC TTGACTTGCC AACACAGAAA 
ACACTAGTTA AGCTAGAAGA GCAGCCCAAA AATATTTCAG TTGAGAATAT AAAGCACAAA 
GCAACTATGA ACAATTCATT GTGGATTGAA AAAAAGCCCA CACAGCCCAC AACTGCAGAA 
CTAGAAGAAA CAAAAGCAAA ACCGTTGGCC CCCATCATGC TGGCATCATG GAATGAACCC 
AGTCTGGATG CTGACACAGA GGAATTATTT TCTGAATCAC AGAGTTACAC TACAACCCCA 
AAAAGAAAAC TTCCTCAATG GTTCGGTCCT GCAAAAGGAA ATGCGGGAAC AAATGCTGTC 
AAAAAAGCAA AAACTGGGAA GAAAAAAGGC ATTTTTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954853.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810307.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa28799
Clone ID Related Accession (Same CDS sequence)	XM_012954853.2
Accession Version	XM_012954853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTGCA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGACTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCTTGAAACT TCAGACACCA 
TGTAAACCTT TGCAAGCGGC TGAACCAGAT GTTTCACCCA AAGAGATGGA ACTTCAGACA 
ACTCTATATG GCAGATTGGT AGTAGCTAGA CAAAAGATTG CTAGCGAGAG AGATATACCG 
CCTGCTGTCC TAGCTACCAA CAAAGTATTA GTAGACATGG CAAAATTGAG GCCAACCACA 
ATCGAAAACA TGAAGAAACT AGACGGAGTG TCAGAAGCCA AGTCAGCAAT GCTTGCCCCC 
CTAGTGGAAG TTGTTAAAGA GTTCTGCATT GCTAATAGTT TGAAGATGGA TATATTCTCA 
AGATCTGGAT CCCAATCTGA GTTGACATCT TCAACTCAAA GGGAGCAAGA CAGCACTACT 
TTGCCAGAGT CACAGCGTAT GTCCTATTCA TTGTTTCAGG AGCAGAATTT GTCTTTGAAA 
AAAGTTGCAG ATGCTCGCTG TTTAAGCATG GCAGCAGTCG GTATGCATTT GTGGCAAGCC 
TTGAAAGCTG GTTATAGTGT CGATGTTCAG AGAGCTGGCC TGACTCCTGG AATTAGGAAG 
CTAATTATAG ATAATATTAA AAATCCTCCC ATTAATTCAG ACCTTTCAAG TTTTAAAGCT 
ATTCGTGAAT CCATTCCTGC TAACATTGAC AGCTACCTCA TTAGAATGGC GATTGCTCTG 
CTTGAGAAGG AGGAGAGCAA TGGGGCCCAA GGACAACTTG ACTTGCCAAC ACAGAAAACA 
CTAGTTAAGC TAGAAGAGCA GCCCAAAAAT ATTTCAGTTG AGAATATAAA GCACAAAGCA 
ACTATGAACA ATTCATTGTG GATTGAAAAA AAGCCCACAC AGCCCACAAC TGCAGAACTA 
GAAGAAACAA AAGCAAAACC GTTGGCCCCC ATCATGCTGG CATCATGGAA TGAACCCAGT 
CTGGATGCTG ACACAGAGGA ATTATTTTCT GAATCACAGA GTTACACTAC AACCCCAAAA 
AGAAAACTTC CTCAATGGTT CGGTCCTGCA AAAGGAAATG CGGGAACAAA TGCTGTCAAA 
AAAGCAAAAA CTGGGAAGAA AAAAGGCATT TTTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810307.1
CDS	327..4625
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSREGVADFEGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPRLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSLKLQTPCKPLQAAEPDVSPKEMELQTTLYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954853.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954853.2

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGA GGGTGTTGCT 
GACTTTGAAG GTATTAAAAG ACAGCTTACT GATCTGTCAA ATGGATTTAT GGATCTGGTG 
AAGGATGTTC CAAGCAGTTT TGGGCATTCT ACAGGGGCTC TAAGAGCTGT GGCTATTCTG 
GAAGACCTTT CAGAGAAATT GGAGGAATTA AGGAGTATTG TTAAAGAGGC CTCAAAAGGA 
GAGGGGAATG GGCTGCTTTG CCATGACTCA GAAGACTGCA GTGAAGAAGA CAAGTATCAA 
AGTATTAATT TAGCATCTAA GGAATTGCTG GCTGAGAATA AAATAGACTA TAAAAATGCT 
GAGAGCAAAA ACAACAAAGA TGTTGACTGC TGCCAAAATG AGAACATGAA ACAAGACTTT 
TTGGGCATAA GTGAAGAAGA ACTTTACATG ATGGAAAGAA AAGATGACGA AAAACAAATC 
AAACCTGGTC AAAAATCAAA TGAGCATTTA TATGATACTA ATGAAGAAAA AAACATGTCA 
TATATAATTG AAAGTGATGA AGAATTTGAA TCTGAAATTA TCAAGTCCCT AGAAGATTTG 
GACAATAGCA TAGAAGAAGC TATTGGAACA AGTGTCCATC AGACAGGCTT AATCCCTGCT 
AAAAGTGTGG ATACTGTTGC AGATGAAGAA GAGGATGAAG GCATAGAGGA AGAAGATGAT 
GATGATGACT GGGATCCATC CATACCAGAA CCTAATGCAG AACAGGTTTC TTGCTTGAAA 
ACATATTTTG GCCATTCAAG TTTTAAACCA GTTCAGTGGA AAGTCATTCA TTCTGTTTTA 
AGAGAAAGAA GAGACAACCT AGTTGTTATG GCTACAGGCT ATGGGAAGAG TTTGTGTTAC 
CAGTTTGCAC CAGTTTATAC ATCTGGCATT GGAATTGTGA TCTGTCCACT TATTTCACTC 
ATGGAGGACC AGGTGTTGCA GCTTGAGATG TCAAATATTC CATCTTGTTT TCTTGGATCT 
GCACAATCAA AAAATGTTTT ACAAGATGTG AAAGCTGGCA GGATGAGAGT AATTTACATG 
ACTCCTGAGT TTTGTTCTGG AGGGATTTCC TTACTTCAGG ATCTTGACAA TAGATATGGT 
ATCACTCTAA TTGCTATTGA TGAAGCACAT TGCATTTCTG AGTGGGGCCA TGATTTCAGA 
AGTGCCTACA GAAGTTTGGG ATCACTGAAG AGAATGCTAC CAACTGTGCC AATTGTGGCC 
TTGACAGCAA CAGCAAGCCC CTCAATTCGT GAAGACATCT CAAAATCTCT CAGTCTCCAT 
AATCCTCAGA TCACATGCAC TAGTTTTGAT CGTCCAAACC TCTACTTAGA TGTTGCAAAG 
AAAACAACAA ACATTTCTAC AGACCTTCAA CAGTTTCTTA TTAAAAAGCA AGGCTCTGGC 
TGGGAGTTTG AAGGAGCCAC AATTGTTTAC TGTCCAACTA GAAAGACATC AGAACAGGTC 
ACAGCAGAGC TAACAAAACT TGGCATTGCT TGTGGAACAT ACCACGCTGG CATGGGAATC 
AAACAAAGAC GAGACGTGCA TCATCGCTTC ATGAGAGATG AAATACAGTG TGTTGTGGCC 
ACTGTTGCAT TTGGGATGGG TATCAACAAG CCAGACATTC GCAAAGTTAT CCACTATGGT 
GCTCCCAAAG AAATGGAGTC ATACTATCAG GAAATTGGAA GAGCTGGACG AGATGGGCTT 
CCTAGCTGTT GTCATGCTCT GTGGACACAA GCAGACATAA ATTTCAATAG ACACATGTTG 
GGTGAGATTC CAAACAATAG TTTTCGAGAG TACAAATTGA AGATGTTGAC AAAGATGGAA 
AAGTATCTGA ACTCAAGCAA TTGTAGACGC AAAATCATCT TGTCTCATTT TGAAGATAAA 
CAACTCCGTA AGGCCTCCTC TGGCATCATG GGGACAGAAA AATGCTGTGA TAACTGTAAG 
ACAAGATTGA GATTTAATAT TTCAATAAAT GACACAGAAG ACAACCTACA AGAGTTTGGT 
CAACAGGCTT ACAAATTTAT TTCAGCAGTA GACATTTTAG GACAGAAATT TGGAACTGGT 
GTCCCAGTGC TCTTTCTTCG TGGTTCAACT TCTCAGCGAC TCCCAGATAG ATTCCGCCAT 
CATCCTCTTT TTAGCTGTGG GAAAGACCAG ACTGAGGCCT TCTGGAAGGT GTTGGCACGC 
CAGCTTATTT CTGAAGGTTA TCTACAGGAG TCTTCAGGCC ATACAAAATT TTCTACCATT 
TGTGGTCTTA CTTCAAAGGG TACAAACTGG TTAATGAAGG CCAGCAACCA GCAGCATCCA 
AGACTCCTCC TGCCATCCAA TGACGAATTA TGCCTTCGAA GAACAACAGT ATCAAATAGT 
ATAAGCCGTG TTCCAAGCCA TTCTTCCATG ATACCACATG CTTCACCAAA TACAAGATCC 
AGTATACCTA AAGCAGGCCC AGAGAAAATC TCATTGAAGG AAAAGTTTTC GTATCAGGAA 
GCTGGAAAGT TGTCCACAGC TGTAGATGTT TCCAAGAGCA GCTTGAAACT TCAGACACCA 
TGTAAACCTT TGCAAGCGGC TGAACCAGAT GTTTCACCCA AAGAGATGGA ACTTCAGACA 
ACTCTATATG GCAGATTGGT AGTAGCTAGA CAAAAGATTG CTAGCGAGAG AGATATACCG 
CCTGCTGTCC TAGCTACCAA CAAAGTATTA GTAGACATGG CAAAATTGAG GCCAACCACA 
ATCGAAAACA TGAAGAAACT AGACGGAGTG TCAGAAGCCA AGTCAGCAAT GCTTGCCCCC 
CTAGTGGAAG TTGTTAAAGA GTTCTGCATT GCTAATAGTT TGAAGATGGA TATATTCTCA 
AGATCTGGAT CCCAATCTGA GTTGACATCT TCAACTCAAA GGGAGCAAGA CAGCACTACT 
TTGCCAGAGT CACAGCGTAT GTCCTATTCA TTGTTTCAGG AGCAGAATTT GTCTTTGAAA 
AAAGTTGCAG ATGCTCGCTG TTTAAGCATG GCAGCAGTCG GTATGCATTT GTGGCAAGCC 
TTGAAAGCTG GTTATAGTGT CGATGTTCAG AGAGCTGGCC TGACTCCTGG AATTAGGAAG 
CTAATTATAG ATAATATTAA AAATCCTCCC ATTAATTCAG ACCTTTCAAG TTTTAAAGCT 
ATTCGTGAAT CCATTCCTGC TAACATTGAC AGCTACCTCA TTAGAATGGC GATTGCTCTG 
CTTGAGAAGG AGGAGAGCAA TGGGGCCCAA GGACAACTTG ACTTGCCAAC ACAGAAAACA 
CTAGTTAAGC TAGAAGAGCA GCCCAAAAAT ATTTCAGTTG AGAATATAAA GCACAAAGCA 
ACTATGAACA ATTCATTGTG GATTGAAAAA AAGCCCACAC AGCCCACAAC TGCAGAACTA 
GAAGAAACAA AAGCAAAACC GTTGGCCCCC ATCATGCTGG CATCATGGAA TGAACCCAGT 
CTGGATGCTG ACACAGAGGA ATTATTTTCT GAATCACAGA GTTACACTAC AACCCCAAAA 
AGAAAACTTC CTCAATGGTT CGGTCCTGCA AAAGGAAATG CGGGAACAAA TGCTGTCAAA 
AAAGCAAAAA CTGGGAAGAA AAAAGGCATT TTTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954855.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810309.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa28800
Clone ID Related Accession (Same CDS sequence)	XM_012954855.2
Accession Version	XM_012954855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAACTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810309.1
CDS	327..4607
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPRLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSSLKLQTPCKPLQAAEPDVSPKEMELQTTLYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954855.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954855.2

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CAGCTTGAAA CTTCAGACAC CATGTAAACC TTTGCAAGCG 
GCTGAACCAG ATGTTTCACC CAAAGAGATG GAACTTCAGA CAACTCTATA TGGCAGATTG 
GTAGTAGCTA GACAAAAGAT TGCTAGCGAG AGAGATATAC CGCCTGCTGT CCTAGCTACC 
AACAAAGTAT TAGTAGACAT GGCAAAATTG AGGCCAACCA CAATCGAAAA CATGAAGAAA 
CTAGACGGAG TGTCAGAAGC CAAGTCAGCA ATGCTTGCCC CCCTAGTGGA AGTTGTTAAA 
GAGTTCTGCA TTGCTAATAG TTTGAAGATG GATATATTCT CAAGATCTGG ATCCCAATCT 
GAGTTGACAT CTTCAACTCA AAGGGAGCAA GACAGCACTA CTTTGCCAGA GTCACAGCGT 
ATGTCCTATT CATTGTTTCA GGAGCAGAAT TTGTCTTTGA AAAAAGTTGC AGATGCTCGC 
TGTTTAAGCA TGGCAGCAGT CGGTATGCAT TTGTGGCAAG CCTTGAAAGC TGGTTATAGT 
GTCGATGTTC AGAGAGCTGG CCTGACTCCT GGAATTAGGA AGCTAATTAT AGATAATATT 
AAAAATCCTC CCATTAATTC AGACCTTTCA AGTTTTAAAG CTATTCGTGA ATCCATTCCT 
GCTAACATTG ACAGCTACCT CATTAGAATG GCGATTGCTC TGCTTGAGAA GGAGGAGAGC 
AATGGGGCCC AAGGACAACT TGACTTGCCA ACACAGAAAA CACTAGTTAA GCTAGAAGAG 
CAGCCCAAAA ATATTTCAGT TGAGAATATA AAGCACAAAG CAACTATGAA CAATTCATTG 
TGGATTGAAA AAAAGCCCAC ACAGCCCACA ACTGCAGAAC TAGAAGAAAC AAAAGCAAAA 
CCGTTGGCCC CCATCATGCT GGCATCATGG AATGAACCCA GTCTGGATGC TGACACAGAG 
GAATTATTTT CTGAATCACA GAGTTACACT ACAACCCCAA AAAGAAAACT TCCTCAATGG 
TTCGGTCCTG CAAAAGGAAA TGCGGGAACA AATGCTGTCA AAAAAGCAAA AACTGGGAAG 
AAAAAAGGCA TTTTTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_012954857.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012810311.1 Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.

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CloneID	OXa28801
Clone ID Related Accession (Same CDS sequence)	XM_012954857.2
Accession Version	XM_012954857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CTTGAAACTT CAGACACCAT GTAAACCTTT GCAAGCGGCT 
GAACCAGATG TTTCACCCAA AGAGATGGAA CTTCAGACAA CTCTATATGG CAGATTGGTA 
GTAGCTAGAC AAAAGATTGC TAGCGAGAGA GATATACCGC CTGCTGTCCT AGCTACCAAC 
AAAGTATTAG TAGACATGGC AAAATTGAGG CCAACCACAA TCGAAAACAT GAAGAAACTA 
GACGGAGTGT CAGAAGCCAA GTCAGCAATG CTTGCCCCCC TAGTGGAAGT TGTTAAAGAG 
TTCTGCATTG CTAATAGTTT GAAGATGGAT ATATTCTCAA GATCTGGATC CCAATCTGAG 
TTGACATCTT CAACTCAAAG GGAGCAAGAC AGCACTACTT TGCCAGAGTC ACAGCGTATG 
TCCTATTCAT TGTTTCAGGA GCAGAATTTG TCTTTGAAAA AAGTTGCAGA TGCTCGCTGT 
TTAAGCATGG CAGCAGTCGG TATGCATTTG TGGCAAGCCT TGAAAGCTGG TTATAGTGTC 
GATGTTCAGA GAGCTGGCCT GACTCCTGGA ATTAGGAAGC TAATTATAGA TAATATTAAA 
AATCCTCCCA TTAATTCAGA CCTTTCAAGT TTTAAAGCTA TTCGTGAATC CATTCCTGCT 
AACATTGACA GCTACCTCAT TAGAATGGCG ATTGCTCTGC TTGAGAAGGA GGAGAGCAAT 
GGGGCCCAAG GACAACTTGA CTTGCCAACA CAGAAAACAC TAGTTAAGCT AGAAGAGCAG 
CCCAAAAATA TTTCAGTTGA GAATATAAAG CACAAAGCAA CTATGAACAA TTCATTGTGG 
ATTGAAAAAA AGCCCACACA GCCCACAACT GCAGAACTAG AAGAAACAAA AGCAAAACCG 
TTGGCCCCCA TCATGCTGGC ATCATGGAAT GAACCCAGTC TGGATGCTGA CACAGAGGAA 
TTATTTTCTG AATCACAGAG TTACACTACA ACCCCAAAAA GAAAACTTCC TCAATGGTTC 
GGTCCTGCAA AAGGAAATGC GGGAACAAAT GCTGTCAAAA AAGCAAAAAC TGGGAAGAAA 
AAAGGCATTT TTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012810311.1
CDS	327..4604
Translation	MTSLQRKLPEWMSVQQKEDSIDDKKKSLWKKNIVEDNLPYLKFNGSIVYSYEGNDCSLLSEDIRSSLQEEDVLGFDIEWPPIYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLRRLLEDESVKKVGVGIEGDQWKLMSDYELKLKGFIELSEVANQKLRCKEKWSLNGLVKHLFKKQLLKDSSIRCSNWDIFPLSTDQKLYAASDAYAGLLIYQKLECMDAHEKVSFCVSRGIKRQLTDLSNGFMDLVKDVPSSFGHSTGALRAVAILEDLSEKLEELRSIVKEASKGEGNGLLCHDSEDCSEEDKYQSINLASKELLAENKIDYKNAESKNNKDVDCCQNENMKQDFLGISEEELYMMERKDDEKQIKPGQKSNEHLYDTNEEKNMSYIIESDEEFESEIIKSLEDLDNSIEEAIGTSVHQTGLIPAKSVDTVADEEEDEGIEEEDDDDDWDPSIPEPNAEQVSCLKTYFGHSSFKPVQWKVIHSVLRERRDNLVVMATGYGKSLCYQFAPVYTSGIGIVICPLISLMEDQVLQLEMSNIPSCFLGSAQSKNVLQDVKAGRMRVIYMTPEFCSGGISLLQDLDNRYGITLIAIDEAHCISEWGHDFRSAYRSLGSLKRMLPTVPIVALTATASPSIREDISKSLSLHNPQITCTSFDRPNLYLDVAKKTTNISTDLQQFLIKKQGSGWEFEGATIVYCPTRKTSEQVTAELTKLGIACGTYHAGMGIKQRRDVHHRFMRDEIQCVVATVAFGMGINKPDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSCCHALWTQADINFNRHMLGEIPNNSFREYKLKMLTKMEKYLNSSNCRRKIILSHFEDKQLRKASSGIMGTEKCCDNCKTRLRFNISINDTEDNLQEFGQQAYKFISAVDILGQKFGTGVPVLFLRGSTSQRLPDRFRHHPLFSCGKDQTEAFWKVLARQLISEGYLQESSGHTKFSTICGLTSKGTNWLMKASNQQHPRLLLPSNDELCLRRTTVSNSISRVPSHSSMIPHASPNTRSSIPKAGPEKISLKEKFSYQEAGKLSTAVDVSKSSLKLQTPCKPLQAAEPDVSPKEMELQTTLYGRLVVARQKIASERDIPPAVLATNKVLVDMAKLRPTTIENMKKLDGVSEAKSAMLAPLVEVVKEFCIANSLKMDIFSRSGSQSELTSSTQREQDSTTLPESQRMSYSLFQEQNLSLKKVADARCLSMAAVGMHLWQALKAGYSVDVQRAGLTPGIRKLIIDNIKNPPINSDLSSFKAIRESIPANIDSYLIRMAIALLEKEESNGAQGQLDLPTQKTLVKLEEQPKNISVENIKHKATMNNSLWIEKKPTQPTTAELEETKAKPLAPIMLASWNEPSLDADTEELFSESQSYTTTPKRKLPQWFGPAKGNAGTNAVKKAKTGKKKGIFD

Target ORF information:

RefSeq Version	XM_012954857.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Werner syndrome, RecQ helicase-like (wrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012954857.2

ORF Insert Sequence:

ATGACTAGCC TTCAGCGTAA GCTTCCAGAG TGGATGTCCG TTCAACAGAA AGAAGACAGT 
ATAGATGATA AAAAGAAATC TCTCTGGAAG AAGAATATTG TGGAAGATAA TTTACCTTAC 
TTGAAATTTA ATGGCTCCAT TGTATACAGC TATGAGGGAA ATGACTGTTC TCTGTTGTCT 
GAAGATATCC GATCAAGCTT GCAAGAAGAG GATGTACTTG GATTTGATAT TGAATGGCCG 
CCTATTTATA CAAAGGGGAA AACTGGAAAA GTTGCTCTTA TTCAAGTGTG TGTTTCTGAG 
AAGAAATGCT ATTTGTTTCA TATTTCACCT ATGGCCGGCT TTCCTAAGGG CTTAAGGAGA 
CTGCTAGAAG ATGAGTCTGT TAAAAAAGTT GGTGTGGGAA TTGAAGGGGA CCAGTGGAAG 
TTAATGAGTG ACTATGAGCT GAAACTTAAA GGCTTTATTG AACTTTCAGA AGTGGCTAAC 
CAAAAACTAA GGTGCAAGGA AAAGTGGAGT CTTAATGGTT TGGTCAAACA CCTTTTCAAG 
AAACAGCTTT TAAAAGATAG CTCTATACGT TGCAGCAACT GGGATATTTT TCCTCTCAGC 
ACAGACCAGA AATTGTATGC TGCCTCTGAT GCTTATGCTG GGCTACTCAT TTACCAGAAA 
CTGGAGTGCA TGGATGCCCA CGAGAAGGTT TCTTTTTGTG TTAGTAGAGG TATTAAAAGA 
CAGCTTACTG ATCTGTCAAA TGGATTTATG GATCTGGTGA AGGATGTTCC AAGCAGTTTT 
GGGCATTCTA CAGGGGCTCT AAGAGCTGTG GCTATTCTGG AAGACCTTTC AGAGAAATTG 
GAGGAATTAA GGAGTATTGT TAAAGAGGCC TCAAAAGGAG AGGGGAATGG GCTGCTTTGC 
CATGACTCAG AAGACTGCAG TGAAGAAGAC AAGTATCAAA GTATTAATTT AGCATCTAAG 
GAATTGCTGG CTGAGAATAA AATAGACTAT AAAAATGCTG AGAGCAAAAA CAACAAAGAT 
GTTGACTGCT GCCAAAATGA GAACATGAAA CAAGACTTTT TGGGCATAAG TGAAGAAGAA 
CTTTACATGA TGGAAAGAAA AGATGACGAA AAACAAATCA AACCTGGTCA AAAATCAAAT 
GAGCATTTAT ATGATACTAA TGAAGAAAAA AACATGTCAT ATATAATTGA AAGTGATGAA 
GAATTTGAAT CTGAAATTAT CAAGTCCCTA GAAGATTTGG ACAATAGCAT AGAAGAAGCT 
ATTGGAACAA GTGTCCATCA GACAGGCTTA ATCCCTGCTA AAAGTGTGGA TACTGTTGCA 
GATGAAGAAG AGGATGAAGG CATAGAGGAA GAAGATGATG ATGATGACTG GGATCCATCC 
ATACCAGAAC CTAATGCAGA ACAGGTTTCT TGCTTGAAAA CATATTTTGG CCATTCAAGT 
TTTAAACCAG TTCAGTGGAA AGTCATTCAT TCTGTTTTAA GAGAAAGAAG AGACAACCTA 
GTTGTTATGG CTACAGGCTA TGGGAAGAGT TTGTGTTACC AGTTTGCACC AGTTTATACA 
TCTGGCATTG GAATTGTGAT CTGTCCACTT ATTTCACTCA TGGAGGACCA GGTGTTGCAG 
CTTGAGATGT CAAATATTCC ATCTTGTTTT CTTGGATCTG CACAATCAAA AAATGTTTTA 
CAAGATGTGA AAGCTGGCAG GATGAGAGTA ATTTACATGA CTCCTGAGTT TTGTTCTGGA 
GGGATTTCCT TACTTCAGGA TCTTGACAAT AGATATGGTA TCACTCTAAT TGCTATTGAT 
GAAGCACATT GCATTTCTGA GTGGGGCCAT GATTTCAGAA GTGCCTACAG AAGTTTGGGA 
TCACTGAAGA GAATGCTACC AACTGTGCCA ATTGTGGCCT TGACAGCAAC AGCAAGCCCC 
TCAATTCGTG AAGACATCTC AAAATCTCTC AGTCTCCATA ATCCTCAGAT CACATGCACT 
AGTTTTGATC GTCCAAACCT CTACTTAGAT GTTGCAAAGA AAACAACAAA CATTTCTACA 
GACCTTCAAC AGTTTCTTAT TAAAAAGCAA GGCTCTGGCT GGGAGTTTGA AGGAGCCACA 
ATTGTTTACT GTCCAACTAG AAAGACATCA GAACAGGTCA CAGCAGAGCT AACAAAACTT 
GGCATTGCTT GTGGAACATA CCACGCTGGC ATGGGAATCA AACAAAGACG AGACGTGCAT 
CATCGCTTCA TGAGAGATGA AATACAGTGT GTTGTGGCCA CTGTTGCATT TGGGATGGGT 
ATCAACAAGC CAGACATTCG CAAAGTTATC CACTATGGTG CTCCCAAAGA AATGGAGTCA 
TACTATCAGG AAATTGGAAG AGCTGGACGA GATGGGCTTC CTAGCTGTTG TCATGCTCTG 
TGGACACAAG CAGACATAAA TTTCAATAGA CACATGTTGG GTGAGATTCC AAACAATAGT 
TTTCGAGAGT ACAAATTGAA GATGTTGACA AAGATGGAAA AGTATCTGAA CTCAAGCAAT 
TGTAGACGCA AAATCATCTT GTCTCATTTT GAAGATAAAC AACTCCGTAA GGCCTCCTCT 
GGCATCATGG GGACAGAAAA ATGCTGTGAT AACTGTAAGA CAAGATTGAG ATTTAATATT 
TCAATAAATG ACACAGAAGA CAACCTACAA GAGTTTGGTC AACAGGCTTA CAAATTTATT 
TCAGCAGTAG ACATTTTAGG ACAGAAATTT GGAACTGGTG TCCCAGTGCT CTTTCTTCGT 
GGTTCAACTT CTCAGCGACT CCCAGATAGA TTCCGCCATC ATCCTCTTTT TAGCTGTGGG 
AAAGACCAGA CTGAGGCCTT CTGGAAGGTG TTGGCACGCC AGCTTATTTC TGAAGGTTAT 
CTACAGGAGT CTTCAGGCCA TACAAAATTT TCTACCATTT GTGGTCTTAC TTCAAAGGGT 
ACAAACTGGT TAATGAAGGC CAGCAACCAG CAGCATCCAA GACTCCTCCT GCCATCCAAT 
GACGAATTAT GCCTTCGAAG AACAACAGTA TCAAATAGTA TAAGCCGTGT TCCAAGCCAT 
TCTTCCATGA TACCACATGC TTCACCAAAT ACAAGATCCA GTATACCTAA AGCAGGCCCA 
GAGAAAATCT CATTGAAGGA AAAGTTTTCG TATCAGGAAG CTGGAAAGTT GTCCACAGCT 
GTAGATGTTT CCAAGAGCAG CTTGAAACTT CAGACACCAT GTAAACCTTT GCAAGCGGCT 
GAACCAGATG TTTCACCCAA AGAGATGGAA CTTCAGACAA CTCTATATGG CAGATTGGTA 
GTAGCTAGAC AAAAGATTGC TAGCGAGAGA GATATACCGC CTGCTGTCCT AGCTACCAAC 
AAAGTATTAG TAGACATGGC AAAATTGAGG CCAACCACAA TCGAAAACAT GAAGAAACTA 
GACGGAGTGT CAGAAGCCAA GTCAGCAATG CTTGCCCCCC TAGTGGAAGT TGTTAAAGAG 
TTCTGCATTG CTAATAGTTT GAAGATGGAT ATATTCTCAA GATCTGGATC CCAATCTGAG 
TTGACATCTT CAACTCAAAG GGAGCAAGAC AGCACTACTT TGCCAGAGTC ACAGCGTATG 
TCCTATTCAT TGTTTCAGGA GCAGAATTTG TCTTTGAAAA AAGTTGCAGA TGCTCGCTGT 
TTAAGCATGG CAGCAGTCGG TATGCATTTG TGGCAAGCCT TGAAAGCTGG TTATAGTGTC 
GATGTTCAGA GAGCTGGCCT GACTCCTGGA ATTAGGAAGC TAATTATAGA TAATATTAAA 
AATCCTCCCA TTAATTCAGA CCTTTCAAGT TTTAAAGCTA TTCGTGAATC CATTCCTGCT 
AACATTGACA GCTACCTCAT TAGAATGGCG ATTGCTCTGC TTGAGAAGGA GGAGAGCAAT 
GGGGCCCAAG GACAACTTGA CTTGCCAACA CAGAAAACAC TAGTTAAGCT AGAAGAGCAG 
CCCAAAAATA TTTCAGTTGA GAATATAAAG CACAAAGCAA CTATGAACAA TTCATTGTGG 
ATTGAAAAAA AGCCCACACA GCCCACAACT GCAGAACTAG AAGAAACAAA AGCAAAACCG 
TTGGCCCCCA TCATGCTGGC ATCATGGAAT GAACCCAGTC TGGATGCTGA CACAGAGGAA 
TTATTTTCTG AATCACAGAG TTACACTACA ACCCCAAAAA GAAAACTTCC TCAATGGTTC 
GGTCCTGCAA AAGGAAATGC GGGAACAAAT GCTGTCAAAA AAGCAAAAAC TGGGAAGAAA 
AAAGGCATTT TTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	wrn
Entrez Gene ID	734043
Full Name	Werner syndrome, RecQ helicase-like
Synonyms	ffa-1,xwrn
General protein information	Preferred Names Werner syndrome, RecQ helicase-like Names Werner syndrome ATP-dependent helicase Werner syndrome homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Danio rerio (zebrafish)	wrn	XP_697980.5
Oryza sativa (rice)	Os07g0681600	NP_001060655.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

wrn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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