tjp2 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_002935597.3	XP_002935643.2	tight junction protein ZO-2 isoform X1
XM_004910777.3	XP_004910834.1	tight junction protein ZO-2 isoform X3
XM_012971796.2	XP_012827250.1	tight junction protein ZO-2 isoform X3
XM_018089352.1	XP_017944841.1	tight junction protein ZO-2 isoform X3
XM_031890789.1	XP_031746649.1	tight junction protein ZO-2 isoform X3
XM_031890787.1	XP_031746647.1	tight junction protein ZO-2 isoform X3
XM_018089356.1	XP_017944845.1	tight junction protein ZO-2 isoform X3
XM_004910776.2	XP_004910833.1	tight junction protein ZO-2 isoform X2
XM_018089353.2	XP_017944842.1	tight junction protein ZO-2 isoform X3
XM_018089353.1	XP_017944842.1	tight junction protein ZO-2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03952	XM_031890787.1 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa03952	XM_031890789.1 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa03952	XM_018089353.2 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa03950	XM_002935597.3 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03951	XM_004910776.2 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03952	XM_018089356.1 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03952	XM_004910777.3 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03952	XM_018089352.1 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03952	XM_012971796.2 Latest version!	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OXa03952	XM_018089353.1	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

tjp2 ( XM_031890789.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746649.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_031890789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746649.1
CDS	80..3601
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_031890789.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890789.1

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_031890787.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746647.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_031890787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746647.1
CDS	93..3614
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_031890787.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890787.1

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_018089353.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944842.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

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CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_018089353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944842.1
CDS	34..3555
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_018089353.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089353.2

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_002935597.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935643.2 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X1, mRNA.

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CloneID	OXa03950
Clone ID Related Accession (Same CDS sequence)	XM_002935597.3
Accession Version	XM_002935597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC 
ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT 
GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA 
GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG 
GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA 
TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA 
AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC 
TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT 
CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT 
TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT 
GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG 
GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC 
AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC 
AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT 
TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT 
GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT 
TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA 
GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA 
AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG 
ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT 
ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT 
CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA 
TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT 
CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA 
GAGGCACCAG TAGAACTGCC TGTAGCTGCT CCCAAGGTGT TTCTGCAGCC AAGCCCAGAA 
GATGAAGAAA TATATGGACC TGGCACCAAA ATGATCAAGT TTGTTAAAGG TGACACAGTT 
GGCATGCGAC TGGCTGGTGG AAATGATGTT GGTATATTTA TTGCTGGAGT GCAGGAGGGC 
AGTGCTGCTG AACAGCAGGG TCTACAAGAA GGAGATCAGC TGCTTAAGGT AAATACTCAA 
GACTTCAGAG GCATAGCTAG AGAGGAGGCA GTTCTCTACC TGTTGGATGT CCCTAAAGGA 
GAAGAAGTAA TTATTCTAAC CCAGAGCAGA TCTGATGTGT ACAAAAACAT ACTTGCCTGT 
GGCCGGGGAG ACTCATTTTT TATAAGGACA CATTTTGAAT ATGACAAAGA AGCTCCACAG 
TTTTTGTCAT TTACAAGAGG CGAGATTTTC CGAGTAGTTG ATACACTCTA TGATGGGAAA 
CTTGGTAACT GGCTGTCTGT GAGGATTGCC AATGAGCTGG AAAAGGGCCT CATTCCAAGT 
AAAAGCAGAG CAGAGCAGTT GGCCAGTGTT CAACTTGCTC AAAGAGGGGC CGCTGGAGAC 
AGAGCGGATT TCTGGAAAAA TCGGGGCCAA AGATCTGGAG CTAAAAAGAA TGCAAGGAAG 
AGCCGTGACG ATCTGACTAC TGTTTCCGCA GTTAGCACTA AGTTTCCAGC TTATGAAAGG 
GTACTGCTTA GAGAAGCTGG CTTTAAAAGA CCAGTTGTGG TATTCGGCCC TATTGCTGAT 
GTAGCAATGG AAAAACTGGC AACTGACATG CCAAGCCTGT ATCAAATTGC CAAGACTGAG 
CCGAAAGATG CTGGCTCTGA CAAGTCTTCT GGCGTTGTGC GGCTGAATAC AGTGAGAATG 
ATAATTGAGC AGGACAAACA TGCATTGCTG GATGTGACTC CCAAAGCTGT GGATCTCTTG 
AACTACACAC AATGGTTCCC TATAGTTGTG TTTTTTAACC CAGACAATAA GCAAGGAGTG 
AAAACCATGA GACAAAGGCT GTGTCCCACA TCGAACAAAA GTTCCAAAAA ACTGTACGAC 
CAGGCAGCTA AACTCAAGAA GACATGTGCT CATCTCATTA CAGCTACTAT CAACTTAAAC 
TCAGCCAATG ACAGCTGGTA TGGCAGCATA AAAGATGTGA TCAGGGAACA GCAGACCCAA 
GCAGTCTGGG TATCGGAAGG AAAGGCTGAT GGAGGAGAAG AGGAGGATGA CCGCATGTCA 
TACCTAACAG CAATGGGAGC TGACTACCTG AGCTGTGACA GTCGTTTAGT CAGTGACTTT 
GAGGACACGG ATGGTGAAGG TGGCGCATAC ACTGACAATG AGCTAGATGA GCTTGTTGAT 
GATCCACTGC CTGTTTCCGC CATTAGCCGT TCATCAGAAC CTGTGCGCCA AGAAGAGACT 
ATAGCTAAAC CTGTGCCAGA GCCACGAGGC CAGATGAAAA AAGCTGGAAG CAGAGAAATG 
CTAAGGGATC CTAGTCCACC TCCGACATTC AAGCCAGAAC CACCAAAGGT AAAAGCACAA 
AGCAAAGATG ATATATATGA CTTGCCACGC TCCTCTGAGG CAAATCGAAG CAGTTCCAGT 
GTAGGCAGCA GTGAGATCTC AGGAGGTTCT GCCAAGGCAA TTCCTCCACC CACTGCAGCT 
AAGCCTGTGT TTCCAGGAAA ACCCATTTTG AAGGCACCAG TTACAGTTCC CTCTCCAGGG 
ACAGAAGAAG TAGCCCCCAT CAAGGAAGAA GCGAATGTTC CCAAATCTGT CTTAGGAAAA 
GTTAAAATAT TTGAAAAAAT GGATTTCAGT GCAAGAGCAC AAAGGCTACA AGAACTGCAA 
GAGGCTCAAA ATGCAAGGTT GGAGCTCGCC CAGAAACTTC CTGATATTTA TGCTGTGCCA 
GTAAAGCCAC AGAAAAATGA ACAGAATCGA CCACAGCCGA CAAGTTCTCG CCCACCAGAG 
CCTCAAAAAC CACCTTCCAG AACCTACCAA GATAACAGAG CGAGTTATGG GAGCGATGAG 
GAGGAAGAAG AATATCGGCG TCAGCTGGCT GACCAATCAA AGCGTGGATA TTATTCCCAG 
CCTTCAAAAT ACAGAGATAC AGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935643.2
CDS	448..4077
Translation	MIAGKMPVRGDRLFVHQFSPPHHGNKNLSAWLGASSMEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_002935597.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935597.3

ORF Insert Sequence:

ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC 
ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT 
GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA 
GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG 
GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA 
TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA 
AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC 
TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT 
CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT 
TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT 
GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG 
GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC 
AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC 
AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT 
TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT 
GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT 
TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA 
GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA 
AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG 
ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT 
ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT 
CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA 
TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT 
CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA 
GAGGCACCAG TAGAACTGCC TGTAGCTGCT CCCAAGGTGT TTCTGCAGCC AAGCCCAGAA 
GATGAAGAAA TATATGGACC TGGCACCAAA ATGATCAAGT TTGTTAAAGG TGACACAGTT 
GGCATGCGAC TGGCTGGTGG AAATGATGTT GGTATATTTA TTGCTGGAGT GCAGGAGGGC 
AGTGCTGCTG AACAGCAGGG TCTACAAGAA GGAGATCAGC TGCTTAAGGT AAATACTCAA 
GACTTCAGAG GCATAGCTAG AGAGGAGGCA GTTCTCTACC TGTTGGATGT CCCTAAAGGA 
GAAGAAGTAA TTATTCTAAC CCAGAGCAGA TCTGATGTGT ACAAAAACAT ACTTGCCTGT 
GGCCGGGGAG ACTCATTTTT TATAAGGACA CATTTTGAAT ATGACAAAGA AGCTCCACAG 
TTTTTGTCAT TTACAAGAGG CGAGATTTTC CGAGTAGTTG ATACACTCTA TGATGGGAAA 
CTTGGTAACT GGCTGTCTGT GAGGATTGCC AATGAGCTGG AAAAGGGCCT CATTCCAAGT 
AAAAGCAGAG CAGAGCAGTT GGCCAGTGTT CAACTTGCTC AAAGAGGGGC CGCTGGAGAC 
AGAGCGGATT TCTGGAAAAA TCGGGGCCAA AGATCTGGAG CTAAAAAGAA TGCAAGGAAG 
AGCCGTGACG ATCTGACTAC TGTTTCCGCA GTTAGCACTA AGTTTCCAGC TTATGAAAGG 
GTACTGCTTA GAGAAGCTGG CTTTAAAAGA CCAGTTGTGG TATTCGGCCC TATTGCTGAT 
GTAGCAATGG AAAAACTGGC AACTGACATG CCAAGCCTGT ATCAAATTGC CAAGACTGAG 
CCGAAAGATG CTGGCTCTGA CAAGTCTTCT GGCGTTGTGC GGCTGAATAC AGTGAGAATG 
ATAATTGAGC AGGACAAACA TGCATTGCTG GATGTGACTC CCAAAGCTGT GGATCTCTTG 
AACTACACAC AATGGTTCCC TATAGTTGTG TTTTTTAACC CAGACAATAA GCAAGGAGTG 
AAAACCATGA GACAAAGGCT GTGTCCCACA TCGAACAAAA GTTCCAAAAA ACTGTACGAC 
CAGGCAGCTA AACTCAAGAA GACATGTGCT CATCTCATTA CAGCTACTAT CAACTTAAAC 
TCAGCCAATG ACAGCTGGTA TGGCAGCATA AAAGATGTGA TCAGGGAACA GCAGACCCAA 
GCAGTCTGGG TATCGGAAGG AAAGGCTGAT GGAGGAGAAG AGGAGGATGA CCGCATGTCA 
TACCTAACAG CAATGGGAGC TGACTACCTG AGCTGTGACA GTCGTTTAGT CAGTGACTTT 
GAGGACACGG ATGGTGAAGG TGGCGCATAC ACTGACAATG AGCTAGATGA GCTTGTTGAT 
GATCCACTGC CTGTTTCCGC CATTAGCCGT TCATCAGAAC CTGTGCGCCA AGAAGAGACT 
ATAGCTAAAC CTGTGCCAGA GCCACGAGGC CAGATGAAAA AAGCTGGAAG CAGAGAAATG 
CTAAGGGATC CTAGTCCACC TCCGACATTC AAGCCAGAAC CACCAAAGGT AAAAGCACAA 
AGCAAAGATG ATATATATGA CTTGCCACGC TCCTCTGAGG CAAATCGAAG CAGTTCCAGT 
GTAGGCAGCA GTGAGATCTC AGGAGGTTCT GCCAAGGCAA TTCCTCCACC CACTGCAGCT 
AAGCCTGTGT TTCCAGGAAA ACCCATTTTG AAGGCACCAG TTACAGTTCC CTCTCCAGGG 
ACAGAAGAAG TAGCCCCCAT CAAGGAAGAA GCGAATGTTC CCAAATCTGT CTTAGGAAAA 
GTTAAAATAT TTGAAAAAAT GGATTTCAGT GCAAGAGCAC AAAGGCTACA AGAACTGCAA 
GAGGCTCAAA ATGCAAGGTT GGAGCTCGCC CAGAAACTTC CTGATATTTA TGCTGTGCCA 
GTAAAGCCAC AGAAAAATGA ACAGAATCGA CCACAGCCGA CAAGTTCTCG CCCACCAGAG 
CCTCAAAAAC CACCTTCCAG AACCTACCAA GATAACAGAG CGAGTTATGG GAGCGATGAG 
GAGGAAGAAG AATATCGGCG TCAGCTGGCT GACCAATCAA AGCGTGGATA TTATTCCCAG 
CCTTCAAAAT ACAGAGATAC AGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_004910776.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910833.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X2, mRNA.

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CloneID	OXa03951
Clone ID Related Accession (Same CDS sequence)	XM_004910776.2
Accession Version	XM_004910776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC 
ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT 
GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA 
GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG 
GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA 
TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA 
AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC 
TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT 
CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT 
TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT 
GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG 
GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC 
AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC 
AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT 
TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT 
GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT 
TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA 
GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA 
AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG 
ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT 
ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT 
CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA 
TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT 
CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA 
GAGGCACCAG AACTGCCTGT AGCTGCTCCC AAGGTGTTTC TGCAGCCAAG CCCAGAAGAT 
GAAGAAATAT ATGGACCTGG CACCAAAATG ATCAAGTTTG TTAAAGGTGA CACAGTTGGC 
ATGCGACTGG CTGGTGGAAA TGATGTTGGT ATATTTATTG CTGGAGTGCA GGAGGGCAGT 
GCTGCTGAAC AGCAGGGTCT ACAAGAAGGA GATCAGCTGC TTAAGGTAAA TACTCAAGAC 
TTCAGAGGCA TAGCTAGAGA GGAGGCAGTT CTCTACCTGT TGGATGTCCC TAAAGGAGAA 
GAAGTAATTA TTCTAACCCA GAGCAGATCT GATGTGTACA AAAACATACT TGCCTGTGGC 
CGGGGAGACT CATTTTTTAT AAGGACACAT TTTGAATATG ACAAAGAAGC TCCACAGTTT 
TTGTCATTTA CAAGAGGCGA GATTTTCCGA GTAGTTGATA CACTCTATGA TGGGAAACTT 
GGTAACTGGC TGTCTGTGAG GATTGCCAAT GAGCTGGAAA AGGGCCTCAT TCCAAGTAAA 
AGCAGAGCAG AGCAGTTGGC CAGTGTTCAA CTTGCTCAAA GAGGGGCCGC TGGAGACAGA 
GCGGATTTCT GGAAAAATCG GGGCCAAAGA TCTGGAGCTA AAAAGAATGC AAGGAAGAGC 
CGTGACGATC TGACTACTGT TTCCGCAGTT AGCACTAAGT TTCCAGCTTA TGAAAGGGTA 
CTGCTTAGAG AAGCTGGCTT TAAAAGACCA GTTGTGGTAT TCGGCCCTAT TGCTGATGTA 
GCAATGGAAA AACTGGCAAC TGACATGCCA AGCCTGTATC AAATTGCCAA GACTGAGCCG 
AAAGATGCTG GCTCTGACAA GTCTTCTGGC GTTGTGCGGC TGAATACAGT GAGAATGATA 
ATTGAGCAGG ACAAACATGC ATTGCTGGAT GTGACTCCCA AAGCTGTGGA TCTCTTGAAC 
TACACACAAT GGTTCCCTAT AGTTGTGTTT TTTAACCCAG ACAATAAGCA AGGAGTGAAA 
ACCATGAGAC AAAGGCTGTG TCCCACATCG AACAAAAGTT CCAAAAAACT GTACGACCAG 
GCAGCTAAAC TCAAGAAGAC ATGTGCTCAT CTCATTACAG CTACTATCAA CTTAAACTCA 
GCCAATGACA GCTGGTATGG CAGCATAAAA GATGTGATCA GGGAACAGCA GACCCAAGCA 
GTCTGGGTAT CGGAAGGAAA GGCTGATGGA GGAGAAGAGG AGGATGACCG CATGTCATAC 
CTAACAGCAA TGGGAGCTGA CTACCTGAGC TGTGACAGTC GTTTAGTCAG TGACTTTGAG 
GACACGGATG GTGAAGGTGG CGCATACACT GACAATGAGC TAGATGAGCT TGTTGATGAT 
CCACTGCCTG TTTCCGCCAT TAGCCGTTCA TCAGAACCTG TGCGCCAAGA AGAGACTATA 
GCTAAACCTG TGCCAGAGCC ACGAGGCCAG ATGAAAAAAG CTGGAAGCAG AGAAATGCTA 
AGGGATCCTA GTCCACCTCC GACATTCAAG CCAGAACCAC CAAAGGTAAA AGCACAAAGC 
AAAGATGATA TATATGACTT GCCACGCTCC TCTGAGGCAA ATCGAAGCAG TTCCAGTGTA 
GGCAGCAGTG AGATCTCAGG AGGTTCTGCC AAGGCAATTC CTCCACCCAC TGCAGCTAAG 
CCTGTGTTTC CAGGAAAACC CATTTTGAAG GCACCAGTTA CAGTTCCCTC TCCAGGGACA 
GAAGAAGTAG CCCCCATCAA GGAAGAAGCG AATGTTCCCA AATCTGTCTT AGGAAAAGTT 
AAAATATTTG AAAAAATGGA TTTCAGTGCA AGAGCACAAA GGCTACAAGA ACTGCAAGAG 
GCTCAAAATG CAAGGTTGGA GCTCGCCCAG AAACTTCCTG ATATTTATGC TGTGCCAGTA 
AAGCCACAGA AAAATGAACA GAATCGACCA CAGCCGACAA GTTCTCGCCC ACCAGAGCCT 
CAAAAACCAC CTTCCAGAAC CTACCAAGAT AACAGAGCGA GTTATGGGAG CGATGAGGAG 
GAAGAAGAAT ATCGGCGTCA GCTGGCTGAC CAATCAAAGC GTGGATATTA TTCCCAGCCT 
TCAAAATACA GAGATACAGA GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910833.1
CDS	448..4074
Translation	MIAGKMPVRGDRLFVHQFSPPHHGNKNLSAWLGASSMEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_004910776.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910776.2

ORF Insert Sequence:

ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC 
ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT 
GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA 
GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG 
GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA 
TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA 
AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC 
TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT 
CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT 
TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT 
GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG 
GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC 
AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC 
AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT 
TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT 
GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT 
TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA 
GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA 
AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG 
ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT 
ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT 
CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA 
TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT 
CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA 
GAGGCACCAG AACTGCCTGT AGCTGCTCCC AAGGTGTTTC TGCAGCCAAG CCCAGAAGAT 
GAAGAAATAT ATGGACCTGG CACCAAAATG ATCAAGTTTG TTAAAGGTGA CACAGTTGGC 
ATGCGACTGG CTGGTGGAAA TGATGTTGGT ATATTTATTG CTGGAGTGCA GGAGGGCAGT 
GCTGCTGAAC AGCAGGGTCT ACAAGAAGGA GATCAGCTGC TTAAGGTAAA TACTCAAGAC 
TTCAGAGGCA TAGCTAGAGA GGAGGCAGTT CTCTACCTGT TGGATGTCCC TAAAGGAGAA 
GAAGTAATTA TTCTAACCCA GAGCAGATCT GATGTGTACA AAAACATACT TGCCTGTGGC 
CGGGGAGACT CATTTTTTAT AAGGACACAT TTTGAATATG ACAAAGAAGC TCCACAGTTT 
TTGTCATTTA CAAGAGGCGA GATTTTCCGA GTAGTTGATA CACTCTATGA TGGGAAACTT 
GGTAACTGGC TGTCTGTGAG GATTGCCAAT GAGCTGGAAA AGGGCCTCAT TCCAAGTAAA 
AGCAGAGCAG AGCAGTTGGC CAGTGTTCAA CTTGCTCAAA GAGGGGCCGC TGGAGACAGA 
GCGGATTTCT GGAAAAATCG GGGCCAAAGA TCTGGAGCTA AAAAGAATGC AAGGAAGAGC 
CGTGACGATC TGACTACTGT TTCCGCAGTT AGCACTAAGT TTCCAGCTTA TGAAAGGGTA 
CTGCTTAGAG AAGCTGGCTT TAAAAGACCA GTTGTGGTAT TCGGCCCTAT TGCTGATGTA 
GCAATGGAAA AACTGGCAAC TGACATGCCA AGCCTGTATC AAATTGCCAA GACTGAGCCG 
AAAGATGCTG GCTCTGACAA GTCTTCTGGC GTTGTGCGGC TGAATACAGT GAGAATGATA 
ATTGAGCAGG ACAAACATGC ATTGCTGGAT GTGACTCCCA AAGCTGTGGA TCTCTTGAAC 
TACACACAAT GGTTCCCTAT AGTTGTGTTT TTTAACCCAG ACAATAAGCA AGGAGTGAAA 
ACCATGAGAC AAAGGCTGTG TCCCACATCG AACAAAAGTT CCAAAAAACT GTACGACCAG 
GCAGCTAAAC TCAAGAAGAC ATGTGCTCAT CTCATTACAG CTACTATCAA CTTAAACTCA 
GCCAATGACA GCTGGTATGG CAGCATAAAA GATGTGATCA GGGAACAGCA GACCCAAGCA 
GTCTGGGTAT CGGAAGGAAA GGCTGATGGA GGAGAAGAGG AGGATGACCG CATGTCATAC 
CTAACAGCAA TGGGAGCTGA CTACCTGAGC TGTGACAGTC GTTTAGTCAG TGACTTTGAG 
GACACGGATG GTGAAGGTGG CGCATACACT GACAATGAGC TAGATGAGCT TGTTGATGAT 
CCACTGCCTG TTTCCGCCAT TAGCCGTTCA TCAGAACCTG TGCGCCAAGA AGAGACTATA 
GCTAAACCTG TGCCAGAGCC ACGAGGCCAG ATGAAAAAAG CTGGAAGCAG AGAAATGCTA 
AGGGATCCTA GTCCACCTCC GACATTCAAG CCAGAACCAC CAAAGGTAAA AGCACAAAGC 
AAAGATGATA TATATGACTT GCCACGCTCC TCTGAGGCAA ATCGAAGCAG TTCCAGTGTA 
GGCAGCAGTG AGATCTCAGG AGGTTCTGCC AAGGCAATTC CTCCACCCAC TGCAGCTAAG 
CCTGTGTTTC CAGGAAAACC CATTTTGAAG GCACCAGTTA CAGTTCCCTC TCCAGGGACA 
GAAGAAGTAG CCCCCATCAA GGAAGAAGCG AATGTTCCCA AATCTGTCTT AGGAAAAGTT 
AAAATATTTG AAAAAATGGA TTTCAGTGCA AGAGCACAAA GGCTACAAGA ACTGCAAGAG 
GCTCAAAATG CAAGGTTGGA GCTCGCCCAG AAACTTCCTG ATATTTATGC TGTGCCAGTA 
AAGCCACAGA AAAATGAACA GAATCGACCA CAGCCGACAA GTTCTCGCCC ACCAGAGCCT 
CAAAAACCAC CTTCCAGAAC CTACCAAGAT AACAGAGCGA GTTATGGGAG CGATGAGGAG 
GAAGAAGAAT ATCGGCGTCA GCTGGCTGAC CAATCAAAGC GTGGATATTA TTCCCAGCCT 
TCAAAATACA GAGATACAGA GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_004910777.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910834.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

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CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_004910777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910834.1
CDS	367..3888
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_004910777.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910777.3

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_012971796.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827250.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_012971796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827250.1
CDS	175..3696
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_012971796.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971796.2

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_018089352.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944841.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

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CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_018089352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944841.1
CDS	107..3628
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_018089352.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089352.1

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_018089356.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944845.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X7, mRNA.

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CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_018089356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944845.1
CDS	285..3806
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_018089356.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089356.1

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp2 ( XM_018089353.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944842.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa03952
Clone ID Related Accession (Same CDS sequence)	XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
Accession Version	XM_018089353.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944842.1
CDS	44..3565
Translation	MEELIWEQFTVTLQKDSKKGFGIAVSGGKDNPHFENGEPSIVISDVLPGGPADGFLQVNDRVVMVNGTLMDNVFHAFAVQQLRKCGKTAVIVVKRPRKVQVTARRSPSPELDPRDIDAVDDDGPFEHRSAYSGYSERSWHSGNGHHSRESSPDRGYSKDQERGRSYDRDQDWVRGRSRERDLDDEREYRRERSRGRSLDRDQSEERRYKRDHKKSVDREESFERSYSGDYSPDRGNGRRLAPDYRSEREMPVNRARDRLQSHSPSPEPHRKSAAAHKGHDKPISVLLSKNKPSEEYGLRLGSQIFIKEMTNTGLARRDGNLHEGDIVLKINGTVTENMSLTDARKLIERSRGKLQLLVQRDASQTLINIPALDDSDSDIGDISDIESNRSNSPLEDSRLQHSDSHSSTEKLKEKSSSKEDPPNRLAKMGAMPTPFKVVGTTETPPPVSVSSVSSENIKEEEQEEEAPVELPVAAPKVFLQPSPEDEEIYGPGTKMIKFVKGDTVGMRLAGGNDVGIFIAGVQEGSAAEQQGLQEGDQLLKVNTQDFRGIAREEAVLYLLDVPKGEEVIILTQSRSDVYKNILACGRGDSFFIRTHFEYDKEAPQFLSFTRGEIFRVVDTLYDGKLGNWLSVRIANELEKGLIPSKSRAEQLASVQLAQRGAAGDRADFWKNRGQRSGAKKNARKSRDDLTTVSAVSTKFPAYERVLLREAGFKRPVVVFGPIADVAMEKLATDMPSLYQIAKTEPKDAGSDKSSGVVRLNTVRMIIEQDKHALLDVTPKAVDLLNYTQWFPIVVFFNPDNKQGVKTMRQRLCPTSNKSSKKLYDQAAKLKKTCAHLITATINLNSANDSWYGSIKDVIREQQTQAVWVSEGKADGGEEEDDRMSYLTAMGADYLSCDSRLVSDFEDTDGEGGAYTDNELDELVDDPLPVSAISRSSEPVRQEETIAKPVPEPRGQMKKAGSREMLRDPSPPPTFKPEPPKVKAQSKDDIYDLPRSSEANRSSSSVGSSEISGGSAKAIPPPTAAKPVFPGKPILKAPVTVPSPGTEEVAPIKEEANVPKSVLGKVKIFEKMDFSARAQRLQELQEAQNARLELAQKLPDIYAVPVKPQKNEQNRPQPTSSRPPEPQKPPSRTYQDNRASYGSDEEEEEYRRQLADQSKRGYYSQPSKYRDTEL

Target ORF information:

RefSeq Version	XM_018089353.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089353.1

ORF Insert Sequence:

ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA 
ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT 
CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG 
CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG 
GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC 
GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT 
AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG 
GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG 
GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT 
GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA 
TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA 
GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG 
TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT 
AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT 
GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC 
CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA 
ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG 
GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA 
GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG 
CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC 
CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA 
ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA 
GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG 
CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA 
GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA 
GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG 
GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT 
GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC 
ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA 
GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC 
TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC 
CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG 
GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG 
AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA 
GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC 
CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT 
GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT 
ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT 
GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT 
AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA 
AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT 
ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA 
CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT 
GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA 
GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT 
GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC 
CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA 
AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG 
GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA 
AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA 
CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT 
CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT 
GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA 
CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT 
TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT 
CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT 
GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA 
TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tjp2
Entrez Gene ID	733995
Full Name	tight junction protein 2
Synonyms	x104,zo-2,zo2
General protein information	Preferred Names tight junction protein 2 Names tight junction protein ZO-2 tight junction protein 2 (zona occludens 2)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	TJP2	XP_003312175.1
Danio rerio (zebrafish)	tjp2a	NP_001188499.1
Xenopus tropicalis (tropical clawed frog)	tjp2	XP_004910833.1
	LOC699586	XP_002808149.1
Canis lupus familiaris (dog)	TJP2	NP_001003204.1
Mus musculus (house mouse)	Tjp2	NP_001185914.1
Gallus gallus (chicken)	TJP2	NP_990249.1
Homo sapiens (human)	TJP2	NP_001163887.1
Rattus norvegicus (Norway rat)	Tjp2	NP_446225.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tjp2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

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