tjp2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tjp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tjp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03952 XM_031890787.1
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03952 XM_031890789.1
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03952 XM_018089353.2
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03950 XM_002935597.3
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03951 XM_004910776.2
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03952 XM_018089356.1
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03952 XM_004910777.3
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03952 XM_018089352.1
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa03952 XM_012971796.2
Latest version!
Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OXa03952 XM_018089353.1 Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_031890789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746649.1
    CDS80..3601
    Translation

    Target ORF information:

    RefSeq Version XM_031890789.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_031890787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746647.1
    CDS93..3614
    Translation

    Target ORF information:

    RefSeq Version XM_031890787.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_018089353.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944842.1
    CDS34..3555
    Translation

    Target ORF information:

    RefSeq Version XM_018089353.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03950
    Clone ID Related Accession (Same CDS sequence) XM_002935597.3
    Accession Version XM_002935597.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
    CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC
    ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT
    GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA
    GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG
    GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA
    TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA
    AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC
    TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT
    CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT
    TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT
    GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG
    GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC
    AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC
    AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT
    TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT
    GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT
    TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA
    GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA
    AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG
    ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT
    ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT
    CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA
    TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT
    CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA
    GAGGCACCAG TAGAACTGCC TGTAGCTGCT CCCAAGGTGT TTCTGCAGCC AAGCCCAGAA
    GATGAAGAAA TATATGGACC TGGCACCAAA ATGATCAAGT TTGTTAAAGG TGACACAGTT
    GGCATGCGAC TGGCTGGTGG AAATGATGTT GGTATATTTA TTGCTGGAGT GCAGGAGGGC
    AGTGCTGCTG AACAGCAGGG TCTACAAGAA GGAGATCAGC TGCTTAAGGT AAATACTCAA
    GACTTCAGAG GCATAGCTAG AGAGGAGGCA GTTCTCTACC TGTTGGATGT CCCTAAAGGA
    GAAGAAGTAA TTATTCTAAC CCAGAGCAGA TCTGATGTGT ACAAAAACAT ACTTGCCTGT
    GGCCGGGGAG ACTCATTTTT TATAAGGACA CATTTTGAAT ATGACAAAGA AGCTCCACAG
    TTTTTGTCAT TTACAAGAGG CGAGATTTTC CGAGTAGTTG ATACACTCTA TGATGGGAAA
    CTTGGTAACT GGCTGTCTGT GAGGATTGCC AATGAGCTGG AAAAGGGCCT CATTCCAAGT
    AAAAGCAGAG CAGAGCAGTT GGCCAGTGTT CAACTTGCTC AAAGAGGGGC CGCTGGAGAC
    AGAGCGGATT TCTGGAAAAA TCGGGGCCAA AGATCTGGAG CTAAAAAGAA TGCAAGGAAG
    AGCCGTGACG ATCTGACTAC TGTTTCCGCA GTTAGCACTA AGTTTCCAGC TTATGAAAGG
    GTACTGCTTA GAGAAGCTGG CTTTAAAAGA CCAGTTGTGG TATTCGGCCC TATTGCTGAT
    GTAGCAATGG AAAAACTGGC AACTGACATG CCAAGCCTGT ATCAAATTGC CAAGACTGAG
    CCGAAAGATG CTGGCTCTGA CAAGTCTTCT GGCGTTGTGC GGCTGAATAC AGTGAGAATG
    ATAATTGAGC AGGACAAACA TGCATTGCTG GATGTGACTC CCAAAGCTGT GGATCTCTTG
    AACTACACAC AATGGTTCCC TATAGTTGTG TTTTTTAACC CAGACAATAA GCAAGGAGTG
    AAAACCATGA GACAAAGGCT GTGTCCCACA TCGAACAAAA GTTCCAAAAA ACTGTACGAC
    CAGGCAGCTA AACTCAAGAA GACATGTGCT CATCTCATTA CAGCTACTAT CAACTTAAAC
    TCAGCCAATG ACAGCTGGTA TGGCAGCATA AAAGATGTGA TCAGGGAACA GCAGACCCAA
    GCAGTCTGGG TATCGGAAGG AAAGGCTGAT GGAGGAGAAG AGGAGGATGA CCGCATGTCA
    TACCTAACAG CAATGGGAGC TGACTACCTG AGCTGTGACA GTCGTTTAGT CAGTGACTTT
    GAGGACACGG ATGGTGAAGG TGGCGCATAC ACTGACAATG AGCTAGATGA GCTTGTTGAT
    GATCCACTGC CTGTTTCCGC CATTAGCCGT TCATCAGAAC CTGTGCGCCA AGAAGAGACT
    ATAGCTAAAC CTGTGCCAGA GCCACGAGGC CAGATGAAAA AAGCTGGAAG CAGAGAAATG
    CTAAGGGATC CTAGTCCACC TCCGACATTC AAGCCAGAAC CACCAAAGGT AAAAGCACAA
    AGCAAAGATG ATATATATGA CTTGCCACGC TCCTCTGAGG CAAATCGAAG CAGTTCCAGT
    GTAGGCAGCA GTGAGATCTC AGGAGGTTCT GCCAAGGCAA TTCCTCCACC CACTGCAGCT
    AAGCCTGTGT TTCCAGGAAA ACCCATTTTG AAGGCACCAG TTACAGTTCC CTCTCCAGGG
    ACAGAAGAAG TAGCCCCCAT CAAGGAAGAA GCGAATGTTC CCAAATCTGT CTTAGGAAAA
    GTTAAAATAT TTGAAAAAAT GGATTTCAGT GCAAGAGCAC AAAGGCTACA AGAACTGCAA
    GAGGCTCAAA ATGCAAGGTT GGAGCTCGCC CAGAAACTTC CTGATATTTA TGCTGTGCCA
    GTAAAGCCAC AGAAAAATGA ACAGAATCGA CCACAGCCGA CAAGTTCTCG CCCACCAGAG
    CCTCAAAAAC CACCTTCCAG AACCTACCAA GATAACAGAG CGAGTTATGG GAGCGATGAG
    GAGGAAGAAG AATATCGGCG TCAGCTGGCT GACCAATCAA AGCGTGGATA TTATTCCCAG
    CCTTCAAAAT ACAGAGATAC AGAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935643.2
    CDS448..4077
    Translation

    Target ORF information:

    RefSeq Version XM_002935597.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935597.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
    CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC
    ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT
    GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA
    GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG
    GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA
    TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA
    AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC
    TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT
    CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT
    TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT
    GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG
    GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC
    AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC
    AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT
    TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT
    GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT
    TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA
    GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA
    AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG
    ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT
    ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT
    CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA
    TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT
    CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA
    GAGGCACCAG TAGAACTGCC TGTAGCTGCT CCCAAGGTGT TTCTGCAGCC AAGCCCAGAA
    GATGAAGAAA TATATGGACC TGGCACCAAA ATGATCAAGT TTGTTAAAGG TGACACAGTT
    GGCATGCGAC TGGCTGGTGG AAATGATGTT GGTATATTTA TTGCTGGAGT GCAGGAGGGC
    AGTGCTGCTG AACAGCAGGG TCTACAAGAA GGAGATCAGC TGCTTAAGGT AAATACTCAA
    GACTTCAGAG GCATAGCTAG AGAGGAGGCA GTTCTCTACC TGTTGGATGT CCCTAAAGGA
    GAAGAAGTAA TTATTCTAAC CCAGAGCAGA TCTGATGTGT ACAAAAACAT ACTTGCCTGT
    GGCCGGGGAG ACTCATTTTT TATAAGGACA CATTTTGAAT ATGACAAAGA AGCTCCACAG
    TTTTTGTCAT TTACAAGAGG CGAGATTTTC CGAGTAGTTG ATACACTCTA TGATGGGAAA
    CTTGGTAACT GGCTGTCTGT GAGGATTGCC AATGAGCTGG AAAAGGGCCT CATTCCAAGT
    AAAAGCAGAG CAGAGCAGTT GGCCAGTGTT CAACTTGCTC AAAGAGGGGC CGCTGGAGAC
    AGAGCGGATT TCTGGAAAAA TCGGGGCCAA AGATCTGGAG CTAAAAAGAA TGCAAGGAAG
    AGCCGTGACG ATCTGACTAC TGTTTCCGCA GTTAGCACTA AGTTTCCAGC TTATGAAAGG
    GTACTGCTTA GAGAAGCTGG CTTTAAAAGA CCAGTTGTGG TATTCGGCCC TATTGCTGAT
    GTAGCAATGG AAAAACTGGC AACTGACATG CCAAGCCTGT ATCAAATTGC CAAGACTGAG
    CCGAAAGATG CTGGCTCTGA CAAGTCTTCT GGCGTTGTGC GGCTGAATAC AGTGAGAATG
    ATAATTGAGC AGGACAAACA TGCATTGCTG GATGTGACTC CCAAAGCTGT GGATCTCTTG
    AACTACACAC AATGGTTCCC TATAGTTGTG TTTTTTAACC CAGACAATAA GCAAGGAGTG
    AAAACCATGA GACAAAGGCT GTGTCCCACA TCGAACAAAA GTTCCAAAAA ACTGTACGAC
    CAGGCAGCTA AACTCAAGAA GACATGTGCT CATCTCATTA CAGCTACTAT CAACTTAAAC
    TCAGCCAATG ACAGCTGGTA TGGCAGCATA AAAGATGTGA TCAGGGAACA GCAGACCCAA
    GCAGTCTGGG TATCGGAAGG AAAGGCTGAT GGAGGAGAAG AGGAGGATGA CCGCATGTCA
    TACCTAACAG CAATGGGAGC TGACTACCTG AGCTGTGACA GTCGTTTAGT CAGTGACTTT
    GAGGACACGG ATGGTGAAGG TGGCGCATAC ACTGACAATG AGCTAGATGA GCTTGTTGAT
    GATCCACTGC CTGTTTCCGC CATTAGCCGT TCATCAGAAC CTGTGCGCCA AGAAGAGACT
    ATAGCTAAAC CTGTGCCAGA GCCACGAGGC CAGATGAAAA AAGCTGGAAG CAGAGAAATG
    CTAAGGGATC CTAGTCCACC TCCGACATTC AAGCCAGAAC CACCAAAGGT AAAAGCACAA
    AGCAAAGATG ATATATATGA CTTGCCACGC TCCTCTGAGG CAAATCGAAG CAGTTCCAGT
    GTAGGCAGCA GTGAGATCTC AGGAGGTTCT GCCAAGGCAA TTCCTCCACC CACTGCAGCT
    AAGCCTGTGT TTCCAGGAAA ACCCATTTTG AAGGCACCAG TTACAGTTCC CTCTCCAGGG
    ACAGAAGAAG TAGCCCCCAT CAAGGAAGAA GCGAATGTTC CCAAATCTGT CTTAGGAAAA
    GTTAAAATAT TTGAAAAAAT GGATTTCAGT GCAAGAGCAC AAAGGCTACA AGAACTGCAA
    GAGGCTCAAA ATGCAAGGTT GGAGCTCGCC CAGAAACTTC CTGATATTTA TGCTGTGCCA
    GTAAAGCCAC AGAAAAATGA ACAGAATCGA CCACAGCCGA CAAGTTCTCG CCCACCAGAG
    CCTCAAAAAC CACCTTCCAG AACCTACCAA GATAACAGAG CGAGTTATGG GAGCGATGAG
    GAGGAAGAAG AATATCGGCG TCAGCTGGCT GACCAATCAA AGCGTGGATA TTATTCCCAG
    CCTTCAAAAT ACAGAGATAC AGAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03951
    Clone ID Related Accession (Same CDS sequence) XM_004910776.2
    Accession Version XM_004910776.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
    CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC
    ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT
    GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA
    GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG
    GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA
    TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA
    AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC
    TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT
    CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT
    TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT
    GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG
    GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC
    AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC
    AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT
    TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT
    GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT
    TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA
    GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA
    AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG
    ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT
    ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT
    CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA
    TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT
    CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA
    GAGGCACCAG AACTGCCTGT AGCTGCTCCC AAGGTGTTTC TGCAGCCAAG CCCAGAAGAT
    GAAGAAATAT ATGGACCTGG CACCAAAATG ATCAAGTTTG TTAAAGGTGA CACAGTTGGC
    ATGCGACTGG CTGGTGGAAA TGATGTTGGT ATATTTATTG CTGGAGTGCA GGAGGGCAGT
    GCTGCTGAAC AGCAGGGTCT ACAAGAAGGA GATCAGCTGC TTAAGGTAAA TACTCAAGAC
    TTCAGAGGCA TAGCTAGAGA GGAGGCAGTT CTCTACCTGT TGGATGTCCC TAAAGGAGAA
    GAAGTAATTA TTCTAACCCA GAGCAGATCT GATGTGTACA AAAACATACT TGCCTGTGGC
    CGGGGAGACT CATTTTTTAT AAGGACACAT TTTGAATATG ACAAAGAAGC TCCACAGTTT
    TTGTCATTTA CAAGAGGCGA GATTTTCCGA GTAGTTGATA CACTCTATGA TGGGAAACTT
    GGTAACTGGC TGTCTGTGAG GATTGCCAAT GAGCTGGAAA AGGGCCTCAT TCCAAGTAAA
    AGCAGAGCAG AGCAGTTGGC CAGTGTTCAA CTTGCTCAAA GAGGGGCCGC TGGAGACAGA
    GCGGATTTCT GGAAAAATCG GGGCCAAAGA TCTGGAGCTA AAAAGAATGC AAGGAAGAGC
    CGTGACGATC TGACTACTGT TTCCGCAGTT AGCACTAAGT TTCCAGCTTA TGAAAGGGTA
    CTGCTTAGAG AAGCTGGCTT TAAAAGACCA GTTGTGGTAT TCGGCCCTAT TGCTGATGTA
    GCAATGGAAA AACTGGCAAC TGACATGCCA AGCCTGTATC AAATTGCCAA GACTGAGCCG
    AAAGATGCTG GCTCTGACAA GTCTTCTGGC GTTGTGCGGC TGAATACAGT GAGAATGATA
    ATTGAGCAGG ACAAACATGC ATTGCTGGAT GTGACTCCCA AAGCTGTGGA TCTCTTGAAC
    TACACACAAT GGTTCCCTAT AGTTGTGTTT TTTAACCCAG ACAATAAGCA AGGAGTGAAA
    ACCATGAGAC AAAGGCTGTG TCCCACATCG AACAAAAGTT CCAAAAAACT GTACGACCAG
    GCAGCTAAAC TCAAGAAGAC ATGTGCTCAT CTCATTACAG CTACTATCAA CTTAAACTCA
    GCCAATGACA GCTGGTATGG CAGCATAAAA GATGTGATCA GGGAACAGCA GACCCAAGCA
    GTCTGGGTAT CGGAAGGAAA GGCTGATGGA GGAGAAGAGG AGGATGACCG CATGTCATAC
    CTAACAGCAA TGGGAGCTGA CTACCTGAGC TGTGACAGTC GTTTAGTCAG TGACTTTGAG
    GACACGGATG GTGAAGGTGG CGCATACACT GACAATGAGC TAGATGAGCT TGTTGATGAT
    CCACTGCCTG TTTCCGCCAT TAGCCGTTCA TCAGAACCTG TGCGCCAAGA AGAGACTATA
    GCTAAACCTG TGCCAGAGCC ACGAGGCCAG ATGAAAAAAG CTGGAAGCAG AGAAATGCTA
    AGGGATCCTA GTCCACCTCC GACATTCAAG CCAGAACCAC CAAAGGTAAA AGCACAAAGC
    AAAGATGATA TATATGACTT GCCACGCTCC TCTGAGGCAA ATCGAAGCAG TTCCAGTGTA
    GGCAGCAGTG AGATCTCAGG AGGTTCTGCC AAGGCAATTC CTCCACCCAC TGCAGCTAAG
    CCTGTGTTTC CAGGAAAACC CATTTTGAAG GCACCAGTTA CAGTTCCCTC TCCAGGGACA
    GAAGAAGTAG CCCCCATCAA GGAAGAAGCG AATGTTCCCA AATCTGTCTT AGGAAAAGTT
    AAAATATTTG AAAAAATGGA TTTCAGTGCA AGAGCACAAA GGCTACAAGA ACTGCAAGAG
    GCTCAAAATG CAAGGTTGGA GCTCGCCCAG AAACTTCCTG ATATTTATGC TGTGCCAGTA
    AAGCCACAGA AAAATGAACA GAATCGACCA CAGCCGACAA GTTCTCGCCC ACCAGAGCCT
    CAAAAACCAC CTTCCAGAAC CTACCAAGAT AACAGAGCGA GTTATGGGAG CGATGAGGAG
    GAAGAAGAAT ATCGGCGTCA GCTGGCTGAC CAATCAAAGC GTGGATATTA TTCCCAGCCT
    TCAAAATACA GAGATACAGA GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910833.1
    CDS448..4074
    Translation

    Target ORF information:

    RefSeq Version XM_004910776.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910776.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGATTGCTG GGAAGATGCC CGTGAGAGGA GACAGGCTAT TTGTCCATCA GTTCTCTCCG 
    CCTCATCACG GCAACAAGAA TTTGTCAGCA TGGCTTGGGG CCTCTAGCAT GGAAGAACTC
    ATATGGGAGC AGTTCACAGT GACCTTGCAA AAGGATAGCA AAAAGGGATT TGGCATTGCT
    GTCTCTGGAG GCAAAGATAA TCCCCATTTT GAAAATGGAG AACCTTCAAT TGTCATCTCA
    GATGTGCTTC CTGGAGGCCC AGCAGATGGA TTCTTACAAG TAAATGATCG TGTGGTTATG
    GTTAATGGGA CACTTATGGA TAATGTGTTC CATGCATTTG CAGTTCAGCA GCTACGGAAA
    TGTGGAAAAA CAGCCGTCAT AGTTGTGAAA AGACCTCGAA AAGTTCAGGT GACTGCAAGA
    AGGTCACCTT CTCCTGAGCT GGACCCTAGA GATATTGATG CTGTAGACGA TGATGGACCC
    TTTGAACACA GAAGTGCTTA CAGTGGCTAC AGCGAGAGGA GTTGGCATAG TGGAAACGGT
    CATCACAGTA GAGAATCGAG TCCAGACAGA GGCTACAGCA AGGACCAGGA AAGAGGTCGT
    TCCTACGACA GGGATCAAGA CTGGGTTCGT GGGCGAAGCC GGGAGAGGGA TCTGGACGAT
    GAGCGAGAAT ACAGGCGGGA GCGCAGTAGG GGTAGAAGTC TAGACCGTGA CCAGAGTGAG
    GAGCGTAGAT ACAAAAGAGA TCACAAAAAG AGTGTGGACA GAGAGGAATC CTTTGAACGC
    AGCTACAGTG GAGACTACAG CCCAGACCGA GGCAACGGCA GGAGACTAGC ACCTGACTAC
    AGGAGTGAGA GGGAGATGCC AGTGAATCGT GCTCGTGATA GGCTTCAGTC CCACAGTCCT
    TCACCTGAAC CACACAGAAA ATCTGCTGCA GCTCACAAGG GACATGATAA GCCCATCAGT
    GTTTTGCTTA GTAAAAACAA GCCCAGTGAA GAATATGGTT TGCGACTTGG AAGTCAGATT
    TTCATAAAAG AAATGACAAA TACAGGTCTT GCAAGGAGAG ATGGAAACCT CCATGAAGGA
    GACATTGTTC TTAAGATTAA TGGCACTGTC ACAGAAAATA TGTCTCTAAC AGATGCTCGA
    AAGCTAATCG AGAGGTCCAG AGGGAAACTT CAGCTTTTAG TGCAGAGGGA TGCCAGCCAG
    ACCCTTATCA ACATCCCTGC ACTAGATGAT AGTGACTCTG ACATAGGAGA CATTTCTGAT
    ATAGAATCCA ATCGTTCCAA TTCACCTCTT GAAGACAGCA GATTGCAGCA TTCTGACTCT
    CATTCTTCCA CTGAAAAGCT GAAAGAAAAA AGCAGCTCCA AAGAGGACCC TCCCAACAGA
    TTAGCTAAAA TGGGAGCCAT GCCTACTCCA TTCAAAGTAG TTGGCACAAC AGAAACTCCT
    CCTCCAGTCT CTGTTAGCTC AGTAAGCTCT GAGAATATAA AGGAAGAAGA ACAGGAAGAA
    GAGGCACCAG AACTGCCTGT AGCTGCTCCC AAGGTGTTTC TGCAGCCAAG CCCAGAAGAT
    GAAGAAATAT ATGGACCTGG CACCAAAATG ATCAAGTTTG TTAAAGGTGA CACAGTTGGC
    ATGCGACTGG CTGGTGGAAA TGATGTTGGT ATATTTATTG CTGGAGTGCA GGAGGGCAGT
    GCTGCTGAAC AGCAGGGTCT ACAAGAAGGA GATCAGCTGC TTAAGGTAAA TACTCAAGAC
    TTCAGAGGCA TAGCTAGAGA GGAGGCAGTT CTCTACCTGT TGGATGTCCC TAAAGGAGAA
    GAAGTAATTA TTCTAACCCA GAGCAGATCT GATGTGTACA AAAACATACT TGCCTGTGGC
    CGGGGAGACT CATTTTTTAT AAGGACACAT TTTGAATATG ACAAAGAAGC TCCACAGTTT
    TTGTCATTTA CAAGAGGCGA GATTTTCCGA GTAGTTGATA CACTCTATGA TGGGAAACTT
    GGTAACTGGC TGTCTGTGAG GATTGCCAAT GAGCTGGAAA AGGGCCTCAT TCCAAGTAAA
    AGCAGAGCAG AGCAGTTGGC CAGTGTTCAA CTTGCTCAAA GAGGGGCCGC TGGAGACAGA
    GCGGATTTCT GGAAAAATCG GGGCCAAAGA TCTGGAGCTA AAAAGAATGC AAGGAAGAGC
    CGTGACGATC TGACTACTGT TTCCGCAGTT AGCACTAAGT TTCCAGCTTA TGAAAGGGTA
    CTGCTTAGAG AAGCTGGCTT TAAAAGACCA GTTGTGGTAT TCGGCCCTAT TGCTGATGTA
    GCAATGGAAA AACTGGCAAC TGACATGCCA AGCCTGTATC AAATTGCCAA GACTGAGCCG
    AAAGATGCTG GCTCTGACAA GTCTTCTGGC GTTGTGCGGC TGAATACAGT GAGAATGATA
    ATTGAGCAGG ACAAACATGC ATTGCTGGAT GTGACTCCCA AAGCTGTGGA TCTCTTGAAC
    TACACACAAT GGTTCCCTAT AGTTGTGTTT TTTAACCCAG ACAATAAGCA AGGAGTGAAA
    ACCATGAGAC AAAGGCTGTG TCCCACATCG AACAAAAGTT CCAAAAAACT GTACGACCAG
    GCAGCTAAAC TCAAGAAGAC ATGTGCTCAT CTCATTACAG CTACTATCAA CTTAAACTCA
    GCCAATGACA GCTGGTATGG CAGCATAAAA GATGTGATCA GGGAACAGCA GACCCAAGCA
    GTCTGGGTAT CGGAAGGAAA GGCTGATGGA GGAGAAGAGG AGGATGACCG CATGTCATAC
    CTAACAGCAA TGGGAGCTGA CTACCTGAGC TGTGACAGTC GTTTAGTCAG TGACTTTGAG
    GACACGGATG GTGAAGGTGG CGCATACACT GACAATGAGC TAGATGAGCT TGTTGATGAT
    CCACTGCCTG TTTCCGCCAT TAGCCGTTCA TCAGAACCTG TGCGCCAAGA AGAGACTATA
    GCTAAACCTG TGCCAGAGCC ACGAGGCCAG ATGAAAAAAG CTGGAAGCAG AGAAATGCTA
    AGGGATCCTA GTCCACCTCC GACATTCAAG CCAGAACCAC CAAAGGTAAA AGCACAAAGC
    AAAGATGATA TATATGACTT GCCACGCTCC TCTGAGGCAA ATCGAAGCAG TTCCAGTGTA
    GGCAGCAGTG AGATCTCAGG AGGTTCTGCC AAGGCAATTC CTCCACCCAC TGCAGCTAAG
    CCTGTGTTTC CAGGAAAACC CATTTTGAAG GCACCAGTTA CAGTTCCCTC TCCAGGGACA
    GAAGAAGTAG CCCCCATCAA GGAAGAAGCG AATGTTCCCA AATCTGTCTT AGGAAAAGTT
    AAAATATTTG AAAAAATGGA TTTCAGTGCA AGAGCACAAA GGCTACAAGA ACTGCAAGAG
    GCTCAAAATG CAAGGTTGGA GCTCGCCCAG AAACTTCCTG ATATTTATGC TGTGCCAGTA
    AAGCCACAGA AAAATGAACA GAATCGACCA CAGCCGACAA GTTCTCGCCC ACCAGAGCCT
    CAAAAACCAC CTTCCAGAAC CTACCAAGAT AACAGAGCGA GTTATGGGAG CGATGAGGAG
    GAAGAAGAAT ATCGGCGTCA GCTGGCTGAC CAATCAAAGC GTGGATATTA TTCCCAGCCT
    TCAAAATACA GAGATACAGA GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_004910777.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910834.1
    CDS367..3888
    Translation

    Target ORF information:

    RefSeq Version XM_004910777.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910777.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_012971796.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012827250.1
    CDS175..3696
    Translation

    Target ORF information:

    RefSeq Version XM_012971796.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971796.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_018089352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944841.1
    CDS107..3628
    Translation

    Target ORF information:

    RefSeq Version XM_018089352.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_018089356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944845.1
    CDS285..3806
    Translation

    Target ORF information:

    RefSeq Version XM_018089356.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03952
    Clone ID Related Accession (Same CDS sequence) XM_004910777.3 , XM_012971796.2 , XM_018089352.1 , XM_031890789.1 , XM_031890787.1 , XM_018089356.1 , XM_018089353.2 , XM_018089353.1
    Accession Version XM_018089353.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944842.1
    CDS44..3565
    Translation

    Target ORF information:

    RefSeq Version XM_018089353.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 2 (tjp2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAAC TCATATGGGA GCAGTTCACA GTGACCTTGC AAAAGGATAG CAAAAAGGGA 
    TTTGGCATTG CTGTCTCTGG AGGCAAAGAT AATCCCCATT TTGAAAATGG AGAACCTTCA
    ATTGTCATCT CAGATGTGCT TCCTGGAGGC CCAGCAGATG GATTCTTACA AGTAAATGAT
    CGTGTGGTTA TGGTTAATGG GACACTTATG GATAATGTGT TCCATGCATT TGCAGTTCAG
    CAGCTACGGA AATGTGGAAA AACAGCCGTC ATAGTTGTGA AAAGACCTCG AAAAGTTCAG
    GTGACTGCAA GAAGGTCACC TTCTCCTGAG CTGGACCCTA GAGATATTGA TGCTGTAGAC
    GATGATGGAC CCTTTGAACA CAGAAGTGCT TACAGTGGCT ACAGCGAGAG GAGTTGGCAT
    AGTGGAAACG GTCATCACAG TAGAGAATCG AGTCCAGACA GAGGCTACAG CAAGGACCAG
    GAAAGAGGTC GTTCCTACGA CAGGGATCAA GACTGGGTTC GTGGGCGAAG CCGGGAGAGG
    GATCTGGACG ATGAGCGAGA ATACAGGCGG GAGCGCAGTA GGGGTAGAAG TCTAGACCGT
    GACCAGAGTG AGGAGCGTAG ATACAAAAGA GATCACAAAA AGAGTGTGGA CAGAGAGGAA
    TCCTTTGAAC GCAGCTACAG TGGAGACTAC AGCCCAGACC GAGGCAACGG CAGGAGACTA
    GCACCTGACT ACAGGAGTGA GAGGGAGATG CCAGTGAATC GTGCTCGTGA TAGGCTTCAG
    TCCCACAGTC CTTCACCTGA ACCACACAGA AAATCTGCTG CAGCTCACAA GGGACATGAT
    AAGCCCATCA GTGTTTTGCT TAGTAAAAAC AAGCCCAGTG AAGAATATGG TTTGCGACTT
    GGAAGTCAGA TTTTCATAAA AGAAATGACA AATACAGGTC TTGCAAGGAG AGATGGAAAC
    CTCCATGAAG GAGACATTGT TCTTAAGATT AATGGCACTG TCACAGAAAA TATGTCTCTA
    ACAGATGCTC GAAAGCTAAT CGAGAGGTCC AGAGGGAAAC TTCAGCTTTT AGTGCAGAGG
    GATGCCAGCC AGACCCTTAT CAACATCCCT GCACTAGATG ATAGTGACTC TGACATAGGA
    GACATTTCTG ATATAGAATC CAATCGTTCC AATTCACCTC TTGAAGACAG CAGATTGCAG
    CATTCTGACT CTCATTCTTC CACTGAAAAG CTGAAAGAAA AAAGCAGCTC CAAAGAGGAC
    CCTCCCAACA GATTAGCTAA AATGGGAGCC ATGCCTACTC CATTCAAAGT AGTTGGCACA
    ACAGAAACTC CTCCTCCAGT CTCTGTTAGC TCAGTAAGCT CTGAGAATAT AAAGGAAGAA
    GAACAGGAAG AAGAGGCACC AGTAGAACTG CCTGTAGCTG CTCCCAAGGT GTTTCTGCAG
    CCAAGCCCAG AAGATGAAGA AATATATGGA CCTGGCACCA AAATGATCAA GTTTGTTAAA
    GGTGACACAG TTGGCATGCG ACTGGCTGGT GGAAATGATG TTGGTATATT TATTGCTGGA
    GTGCAGGAGG GCAGTGCTGC TGAACAGCAG GGTCTACAAG AAGGAGATCA GCTGCTTAAG
    GTAAATACTC AAGACTTCAG AGGCATAGCT AGAGAGGAGG CAGTTCTCTA CCTGTTGGAT
    GTCCCTAAAG GAGAAGAAGT AATTATTCTA ACCCAGAGCA GATCTGATGT GTACAAAAAC
    ATACTTGCCT GTGGCCGGGG AGACTCATTT TTTATAAGGA CACATTTTGA ATATGACAAA
    GAAGCTCCAC AGTTTTTGTC ATTTACAAGA GGCGAGATTT TCCGAGTAGT TGATACACTC
    TATGATGGGA AACTTGGTAA CTGGCTGTCT GTGAGGATTG CCAATGAGCT GGAAAAGGGC
    CTCATTCCAA GTAAAAGCAG AGCAGAGCAG TTGGCCAGTG TTCAACTTGC TCAAAGAGGG
    GCCGCTGGAG ACAGAGCGGA TTTCTGGAAA AATCGGGGCC AAAGATCTGG AGCTAAAAAG
    AATGCAAGGA AGAGCCGTGA CGATCTGACT ACTGTTTCCG CAGTTAGCAC TAAGTTTCCA
    GCTTATGAAA GGGTACTGCT TAGAGAAGCT GGCTTTAAAA GACCAGTTGT GGTATTCGGC
    CCTATTGCTG ATGTAGCAAT GGAAAAACTG GCAACTGACA TGCCAAGCCT GTATCAAATT
    GCCAAGACTG AGCCGAAAGA TGCTGGCTCT GACAAGTCTT CTGGCGTTGT GCGGCTGAAT
    ACAGTGAGAA TGATAATTGA GCAGGACAAA CATGCATTGC TGGATGTGAC TCCCAAAGCT
    GTGGATCTCT TGAACTACAC ACAATGGTTC CCTATAGTTG TGTTTTTTAA CCCAGACAAT
    AAGCAAGGAG TGAAAACCAT GAGACAAAGG CTGTGTCCCA CATCGAACAA AAGTTCCAAA
    AAACTGTACG ACCAGGCAGC TAAACTCAAG AAGACATGTG CTCATCTCAT TACAGCTACT
    ATCAACTTAA ACTCAGCCAA TGACAGCTGG TATGGCAGCA TAAAAGATGT GATCAGGGAA
    CAGCAGACCC AAGCAGTCTG GGTATCGGAA GGAAAGGCTG ATGGAGGAGA AGAGGAGGAT
    GACCGCATGT CATACCTAAC AGCAATGGGA GCTGACTACC TGAGCTGTGA CAGTCGTTTA
    GTCAGTGACT TTGAGGACAC GGATGGTGAA GGTGGCGCAT ACACTGACAA TGAGCTAGAT
    GAGCTTGTTG ATGATCCACT GCCTGTTTCC GCCATTAGCC GTTCATCAGA ACCTGTGCGC
    CAAGAAGAGA CTATAGCTAA ACCTGTGCCA GAGCCACGAG GCCAGATGAA AAAAGCTGGA
    AGCAGAGAAA TGCTAAGGGA TCCTAGTCCA CCTCCGACAT TCAAGCCAGA ACCACCAAAG
    GTAAAAGCAC AAAGCAAAGA TGATATATAT GACTTGCCAC GCTCCTCTGA GGCAAATCGA
    AGCAGTTCCA GTGTAGGCAG CAGTGAGATC TCAGGAGGTT CTGCCAAGGC AATTCCTCCA
    CCCACTGCAG CTAAGCCTGT GTTTCCAGGA AAACCCATTT TGAAGGCACC AGTTACAGTT
    CCCTCTCCAG GGACAGAAGA AGTAGCCCCC ATCAAGGAAG AAGCGAATGT TCCCAAATCT
    GTCTTAGGAA AAGTTAAAAT ATTTGAAAAA ATGGATTTCA GTGCAAGAGC ACAAAGGCTA
    CAAGAACTGC AAGAGGCTCA AAATGCAAGG TTGGAGCTCG CCCAGAAACT TCCTGATATT
    TATGCTGTGC CAGTAAAGCC ACAGAAAAAT GAACAGAATC GACCACAGCC GACAAGTTCT
    CGCCCACCAG AGCCTCAAAA ACCACCTTCC AGAACCTACC AAGATAACAG AGCGAGTTAT
    GGGAGCGATG AGGAGGAAGA AGAATATCGG CGTCAGCTGG CTGACCAATC AAAGCGTGGA
    TATTATTCCC AGCCTTCAAA ATACAGAGAT ACAGAGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.