e4f1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following e4f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e4f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa20911 NM_001078698.1
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa27354 XM_012970381.2
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa27351 XM_012970378.2
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa27352 XM_012970379.2
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa27353 XM_012970380.2
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa66005 XM_012970378.3
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa66006 XM_012970379.3
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa66007 XM_012970380.3
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa66008 XM_012970381.3
Latest version!
Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa20911
    Clone ID Related Accession (Same CDS sequence) NM_001078698.1
    Accession Version NM_001078698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121560.1. ##Evidence-Data-START## Transcript exon combination :: BC121560.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC 
    GCCAATATAT TGAGAGCCCA CATGATCACC CACAGCACAA AAAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA TGGGACAGCA
    GACAGCGATG ATCCAGCTCT GAAATGTCCA AAGTGTAATA CCATTTGCAT AAGTTCCAAT
    GCTTTGAGTG TCCACTTGAA AGGGCATGAA GGGTGCATGC TGTACTCGTG TGAAGTGTGC
    GGCAGGGAGT TCCTGAAGCG CCACCTGCTC AGGAAGCACC AGGAGTGCCA TTTCAGCGAG
    CGCAAATACA AGTGCGGAGA GTGCGGCAAA ATGTACAAGA CTGTCGCGCA CGTCAAGGGC
    CACATGAGAG TGCACTCGGA TGATCGGCCG TACCCTTGTC CCAAGTGTGG CAAGAGATAC
    AAAACCAAGA ATGCACAGCA GGTCCACTCT CGCACCCACC TGGATGAGAA GCCATATGTC
    TGCCAGTACT GCCCCAACAG CTTCCGGGAG AAGGGCTCTC TTGTCCGGCA CATCAGGCAC
    CACACTGGTG AGAAGCCCTT CAAGTGCCAC AAATGTGGCC GAGGCTTTGC CGAGCATGGA
    ACTCTAAATC GACATTTAAA AACCAAAGGA GGATGCATGC TGCTATTGTC GCTGAAAAAA
    AAGCCTGATG GTGGGTCTAG CGCTGAAGAG AGCAGCGCCC TGCAGAACGA GGAGACCGCT
    GGGGCATCAT CTGAGGAGTC GGAGGAACCT CACACGGTGC TAGTGGAGTT TTCTTCAGTG
    GTCGCCGATA CTCAGGAATA TATCATAGAG ACTGCTGCTG AAGACTCCGA GGCCAGTGAA
    GCTGCTGAGC TCATCCAGGA TTCCGGATCC CAAGAGGTTG ACGGCCACAT CTATTCAGTG
    GTTCAGCAGC TCGTAAGCGA CGCAAGCCCG GGCCAACAGA TCATTGTCCG CAACGTGTCT
    ATCGACGGCG AGGAGAGGGT AGAGGAAGTG CCCACTACTG ACACCATTGC TATAGCGACT
    CCTGAACTGC TCACGGAGCA AGTTGCCTTG ACTTTGGCCT CTGCCATTGA GGACGGTGCT
    GTAGTGGCCA AGGAGGAGCC CATGGAGGAG CAACAAATTC CAATCCCAGA GGAAGTGGAG
    ATGATGGAGC ACGCAGGGGA ATTTGTCATT GAGTCCCATG ACGGAGATCT AGAAGTTCAG
    ACAGTCATTG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072166.1
    CDS477..1931
    Translation

    Target ORF information:

    RefSeq Version NM_001078698.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001078698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC 
    GCCAATATAT TGAGAGCCCA CATGATCACC CACAGCACAA AAAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA TGGGACAGCA
    GACAGCGATG ATCCAGCTCT GAAATGTCCA AAGTGTAATA CCATTTGCAT AAGTTCCAAT
    GCTTTGAGTG TCCACTTGAA AGGGCATGAA GGGTGCATGC TGTACTCGTG TGAAGTGTGC
    GGCAGGGAGT TCCTGAAGCG CCACCTGCTC AGGAAGCACC AGGAGTGCCA TTTCAGCGAG
    CGCAAATACA AGTGCGGAGA GTGCGGCAAA ATGTACAAGA CTGTCGCGCA CGTCAAGGGC
    CACATGAGAG TGCACTCGGA TGATCGGCCG TACCCTTGTC CCAAGTGTGG CAAGAGATAC
    AAAACCAAGA ATGCACAGCA GGTCCACTCT CGCACCCACC TGGATGAGAA GCCATATGTC
    TGCCAGTACT GCCCCAACAG CTTCCGGGAG AAGGGCTCTC TTGTCCGGCA CATCAGGCAC
    CACACTGGTG AGAAGCCCTT CAAGTGCCAC AAATGTGGCC GAGGCTTTGC CGAGCATGGA
    ACTCTAAATC GACATTTAAA AACCAAAGGA GGATGCATGC TGCTATTGTC GCTGAAAAAA
    AAGCCTGATG GTGGGTCTAG CGCTGAAGAG AGCAGCGCCC TGCAGAACGA GGAGACCGCT
    GGGGCATCAT CTGAGGAGTC GGAGGAACCT CACACGGTGC TAGTGGAGTT TTCTTCAGTG
    GTCGCCGATA CTCAGGAATA TATCATAGAG ACTGCTGCTG AAGACTCCGA GGCCAGTGAA
    GCTGCTGAGC TCATCCAGGA TTCCGGATCC CAAGAGGTTG ACGGCCACAT CTATTCAGTG
    GTTCAGCAGC TCGTAAGCGA CGCAAGCCCG GGCCAACAGA TCATTGTCCG CAACGTGTCT
    ATCGACGGCG AGGAGAGGGT AGAGGAAGTG CCCACTACTG ACACCATTGC TATAGCGACT
    CCTGAACTGC TCACGGAGCA AGTTGCCTTG ACTTTGGCCT CTGCCATTGA GGACGGTGCT
    GTAGTGGCCA AGGAGGAGCC CATGGAGGAG CAACAAATTC CAATCCCAGA GGAAGTGGAG
    ATGATGGAGC ACGCAGGGGA ATTTGTCATT GAGTCCCATG ACGGAGATCT AGAAGTTCAG
    ACAGTCATTG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27354
    Clone ID Related Accession (Same CDS sequence) XM_012970381.2
    Accession Version XM_012970381.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGATGGGACA
    GCAGACAGCG ATGATCCAGC TCTGAAATGT CCAAAGTGTA ATACCATTTG CATAAGTTCC
    AATGCTTTGA GTGTCCACTT GAAAGGGCAT GAAGGGTGCA TGCTGTACTC GTGTGAAGTG
    TGCGGCAGGG AGTTCCTGAA GCGCCACCTG CTCAGGAAGC ACCAGGAGTG CCATTTCAGC
    GAGCGCAAAT ACAAGTGCGG AGAGTGCGGC AAAATGTACA AGACTGTCGC GCACGTCAAG
    GGCCACATGA GAGTGCACTC GGATGATCGG CCGTACCCTT GTCCCAAGTG TGGCAAGAGA
    TACAAAACCA AGAATGCACA GCAGGTCCAC TCTCGCACCC ACCTGGATGA GAAGCCATAT
    GTCTGCCAGT ACTGCCCCAA CAGCTTCCGG GAGAAGGGCT CTCTTGTCCG GCACATCAGG
    CACCACACTG GTGAGAAGCC CTTCAAGTGC CACAAATGTG GCCGAGGCTT TGCCGAGCAT
    GGAACTCTAA ATCGACATTT AAAAACCAAA GGAGGATGCA TGCTGCTATT GTCGCTGAAA
    AAAAAGCCTG ATGGTGGGTC TAGCGCTGAA GAGAGCAGCG CCCTGCAGAA CGAGGAGACC
    GCTGGGGCAT CATCTGAGGA GTCGGAGGAA CCTCACACGG TGCTAGTGGA GTTTTCTTCA
    GTGGTCGCCG ATACTCAGGA ATATATCATA GAGACTGCTG CTGAAGACTC CGAGGCCAGT
    GAAGCTGCTG AGCTCATCCA GGATTCCGGA TCCCAAGAGG TTGACGGCCA CATCTATTCA
    GTGGTTCAGC AGCTCGTAAG CGACGCAAGC CCGGGCCAAC AGATCATTGT CCGCAACGTG
    TCTATCGACG GCGAGGAGAG GGTAGAGGAA GTGCCCACTA CTGACACCAT TGCTATAGCG
    ACTCCTGAAC TGCTCACGGA GCAAGTTGCC TTGACTTTGG CCTCTGCCAT TGAGGACGGT
    GCTGTAGTGG CCAAGGAGGA GCCCATGGAG GAGCAACAAA TTCCAATCCC AGAGGAAGTG
    GAGATGATGG AGCACGCAGG GGAATTTGTC ATTGAGTCCC ATGACGGAGA TCTAGAAGTT
    CAGACAGTCA TTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825835.1
    CDS106..2043
    Translation

    Target ORF information:

    RefSeq Version XM_012970381.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970381.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGATGGGACA
    GCAGACAGCG ATGATCCAGC TCTGAAATGT CCAAAGTGTA ATACCATTTG CATAAGTTCC
    AATGCTTTGA GTGTCCACTT GAAAGGGCAT GAAGGGTGCA TGCTGTACTC GTGTGAAGTG
    TGCGGCAGGG AGTTCCTGAA GCGCCACCTG CTCAGGAAGC ACCAGGAGTG CCATTTCAGC
    GAGCGCAAAT ACAAGTGCGG AGAGTGCGGC AAAATGTACA AGACTGTCGC GCACGTCAAG
    GGCCACATGA GAGTGCACTC GGATGATCGG CCGTACCCTT GTCCCAAGTG TGGCAAGAGA
    TACAAAACCA AGAATGCACA GCAGGTCCAC TCTCGCACCC ACCTGGATGA GAAGCCATAT
    GTCTGCCAGT ACTGCCCCAA CAGCTTCCGG GAGAAGGGCT CTCTTGTCCG GCACATCAGG
    CACCACACTG GTGAGAAGCC CTTCAAGTGC CACAAATGTG GCCGAGGCTT TGCCGAGCAT
    GGAACTCTAA ATCGACATTT AAAAACCAAA GGAGGATGCA TGCTGCTATT GTCGCTGAAA
    AAAAAGCCTG ATGGTGGGTC TAGCGCTGAA GAGAGCAGCG CCCTGCAGAA CGAGGAGACC
    GCTGGGGCAT CATCTGAGGA GTCGGAGGAA CCTCACACGG TGCTAGTGGA GTTTTCTTCA
    GTGGTCGCCG ATACTCAGGA ATATATCATA GAGACTGCTG CTGAAGACTC CGAGGCCAGT
    GAAGCTGCTG AGCTCATCCA GGATTCCGGA TCCCAAGAGG TTGACGGCCA CATCTATTCA
    GTGGTTCAGC AGCTCGTAAG CGACGCAAGC CCGGGCCAAC AGATCATTGT CCGCAACGTG
    TCTATCGACG GCGAGGAGAG GGTAGAGGAA GTGCCCACTA CTGACACCAT TGCTATAGCG
    ACTCCTGAAC TGCTCACGGA GCAAGTTGCC TTGACTTTGG CCTCTGCCAT TGAGGACGGT
    GCTGTAGTGG CCAAGGAGGA GCCCATGGAG GAGCAACAAA TTCCAATCCC AGAGGAAGTG
    GAGATGATGG AGCACGCAGG GGAATTTGTC ATTGAGTCCC ATGACGGAGA TCTAGAAGTT
    CAGACAGTCA TTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27351
    Clone ID Related Accession (Same CDS sequence) XM_012970378.2
    Accession Version XM_012970378.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGGCT
    GCGGCTGCTG AGCGGTGTGC AGTAGATGGG GAGCTGAGCA GTGACAGTCC CCTCCATCAT
    ATGAACAGCT CCAGTGTTGT GATTGAGACT GTGTCCATGC AGGATGTGGA GAGCGAGGAA
    ATAATCATAG CCTCAGCAGA TGAGCCACAA AGTGGCGAAA TGGACGAGTT TGTTAACGGC
    CAGAAAACGA TAGATGGGAC AGCAGACAGC GATGATCCAG CTCTGAAATG TCCAAAGTGT
    AATACCATTT GCATAAGTTC CAATGCTTTG AGTGTCCACT TGAAAGGGCA TGAAGGGTGC
    ATGCTGTACT CGTGTGAAGT GTGCGGCAGG GAGTTCCTGA AGCGCCACCT GCTCAGGAAG
    CACCAGGAGT GCCATTTCAG CGAGCGCAAA TACAAGTGCG GAGAGTGCGG CAAAATGTAC
    AAGACTGTCG CGCACGTCAA GGGCCACATG AGAGTGCACT CGGATGATCG GCCGTACCCT
    TGTCCCAAGT GTGGCAAGAG ATACAAAACC AAGAATGCAC AGCAGGTCCA CTCTCGCACC
    CACCTGGATG AGAAGCCATA TGTCTGCCAG TACTGCCCCA ACAGCTTCCG GGAGAAGGGC
    TCTCTTGTCC GGCACATCAG GCACCACACT GGTGAGAAGC CCTTCAAGTG CCACAAATGT
    GGCCGAGGCT TTGCCGAGCA TGGAACTCTA AATCGACATT TAAAAACCAA AGGAGGATGC
    ATGCTGCTAT TGTCGCTGAA AAAAAAGCCT GATGGTGGGT CTAGCGCTGA AGAGAGCAGC
    GCCCTGCAGA ACGAGGAGAC CGCTGGGGCA TCATCTGAGG AGTCGGAGGA ACCTCACACG
    GTGCTAGTGG AGTTTTCTTC AGTGGTCGCC GATACTCAGG AATATATCAT AGAGACTGCT
    GCTGAAGACT CCGAGGCCAG TGAAGCTGCT GAGCTCATCC AGGATTCCGG ATCCCAAGAG
    GTTGACGGCC ACATCTATTC AGTGGTTCAG CAGCTCGTAA GCGACGCAAG CCCGGGCCAA
    CAGATCATTG TCCGCAACGT GTCTATCGAC GGCGAGGAGA GGGTAGAGGA AGTGCCCACT
    ACTGACACCA TTGCTATAGC GACTCCTGAA CTGCTCACGG AGCAAGTTGC CTTGACTTTG
    GCCTCTGCCA TTGAGGACGG TGCTGTAGTG GCCAAGGAGG AGCCCATGGA GGAGCAACAA
    ATTCCAATCC CAGAGGAAGT GGAGATGATG GAGCACGCAG GGGAATTTGT CATTGAGTCC
    CATGACGGAG ATCTAGAAGT TCAGACAGTC ATTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825832.1
    CDS107..2425
    Translation

    Target ORF information:

    RefSeq Version XM_012970378.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970378.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGGCT
    GCGGCTGCTG AGCGGTGTGC AGTAGATGGG GAGCTGAGCA GTGACAGTCC CCTCCATCAT
    ATGAACAGCT CCAGTGTTGT GATTGAGACT GTGTCCATGC AGGATGTGGA GAGCGAGGAA
    ATAATCATAG CCTCAGCAGA TGAGCCACAA AGTGGCGAAA TGGACGAGTT TGTTAACGGC
    CAGAAAACGA TAGATGGGAC AGCAGACAGC GATGATCCAG CTCTGAAATG TCCAAAGTGT
    AATACCATTT GCATAAGTTC CAATGCTTTG AGTGTCCACT TGAAAGGGCA TGAAGGGTGC
    ATGCTGTACT CGTGTGAAGT GTGCGGCAGG GAGTTCCTGA AGCGCCACCT GCTCAGGAAG
    CACCAGGAGT GCCATTTCAG CGAGCGCAAA TACAAGTGCG GAGAGTGCGG CAAAATGTAC
    AAGACTGTCG CGCACGTCAA GGGCCACATG AGAGTGCACT CGGATGATCG GCCGTACCCT
    TGTCCCAAGT GTGGCAAGAG ATACAAAACC AAGAATGCAC AGCAGGTCCA CTCTCGCACC
    CACCTGGATG AGAAGCCATA TGTCTGCCAG TACTGCCCCA ACAGCTTCCG GGAGAAGGGC
    TCTCTTGTCC GGCACATCAG GCACCACACT GGTGAGAAGC CCTTCAAGTG CCACAAATGT
    GGCCGAGGCT TTGCCGAGCA TGGAACTCTA AATCGACATT TAAAAACCAA AGGAGGATGC
    ATGCTGCTAT TGTCGCTGAA AAAAAAGCCT GATGGTGGGT CTAGCGCTGA AGAGAGCAGC
    GCCCTGCAGA ACGAGGAGAC CGCTGGGGCA TCATCTGAGG AGTCGGAGGA ACCTCACACG
    GTGCTAGTGG AGTTTTCTTC AGTGGTCGCC GATACTCAGG AATATATCAT AGAGACTGCT
    GCTGAAGACT CCGAGGCCAG TGAAGCTGCT GAGCTCATCC AGGATTCCGG ATCCCAAGAG
    GTTGACGGCC ACATCTATTC AGTGGTTCAG CAGCTCGTAA GCGACGCAAG CCCGGGCCAA
    CAGATCATTG TCCGCAACGT GTCTATCGAC GGCGAGGAGA GGGTAGAGGA AGTGCCCACT
    ACTGACACCA TTGCTATAGC GACTCCTGAA CTGCTCACGG AGCAAGTTGC CTTGACTTTG
    GCCTCTGCCA TTGAGGACGG TGCTGTAGTG GCCAAGGAGG AGCCCATGGA GGAGCAACAA
    ATTCCAATCC CAGAGGAAGT GGAGATGATG GAGCACGCAG GGGAATTTGT CATTGAGTCC
    CATGACGGAG ATCTAGAAGT TCAGACAGTC ATTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27352
    Clone ID Related Accession (Same CDS sequence) XM_012970379.2
    Accession Version XM_012970379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGGTAGT GCCCTCTATA GAAGAGACTG AAACCATCTC CCAAGTCATT
    GTGGAATCCA CAGAAGATGT CAGTAATGAT CCTGATGAAG AAGTGGAGGA TATTAAGGAC
    ATCCTCAGTG AGGATAGCGC TCTAGGTGGG CAAAGAGATG TGGAGGAGTT GGAAGTTGAA
    TGGGATGAGA GTCGAGGGGA TCCGGATCAA GATGATTATG ATAATTTGGG CCTAGTGACA
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC
    GCCAATATAT TGAGAGCCCA CATGATCACC CACAGCACAA AAAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA AAAGCTAGTC
    CCTACCAAAG ATGCCAACAG CAAGGTGCCG GGCGCTTCCT GCCAACTTGC CAGCGATACC
    AAGGACTCTT CCCAAGTGAT CCACCTTTTG ACAGATGCCA ATGGTAACAT TTTGCAGGAA
    GTTCACATCC AGGTTCAAGC ACATGACAGT GAACCAGTAT CACTTGTGCC AGAGGCTGCG
    GCTGCTGAGC GGTGTGCAGT AGATGGGGAG CTGAGCAGTG ACAGTCCCCT CCATCATATG
    AACAGCTCCA GTGTTGTGAT TGAGACTGTG TCCATGCAGG ATGTGGAGAG CGAGGAAATA
    ATCATAGCCT CAGCAGATGA GCCACAAAGT GGCGAAATGG ACGAGTTTGT TAACGGCCAG
    AAAACGATAG ATGGGACAGC AGACAGCGAT GATCCAGCTC TGAAATGTCC AAAGTGTAAT
    ACCATTTGCA TAAGTTCCAA TGCTTTGAGT GTCCACTTGA AAGGGCATGA AGGGTGCATG
    CTGTACTCGT GTGAAGTGTG CGGCAGGGAG TTCCTGAAGC GCCACCTGCT CAGGAAGCAC
    CAGGAGTGCC ATTTCAGCGA GCGCAAATAC AAGTGCGGAG AGTGCGGCAA AATGTACAAG
    ACTGTCGCGC ACGTCAAGGG CCACATGAGA GTGCACTCGG ATGATCGGCC GTACCCTTGT
    CCCAAGTGTG GCAAGAGATA CAAAACCAAG AATGCACAGC AGGTCCACTC TCGCACCCAC
    CTGGATGAGA AGCCATATGT CTGCCAGTAC TGCCCCAACA GCTTCCGGGA GAAGGGCTCT
    CTTGTCCGGC ACATCAGGCA CCACACTGGT GAGAAGCCCT TCAAGTGCCA CAAATGTGGC
    CGAGGCTTTG CCGAGCATGG AACTCTAAAT CGACATTTAA AAACCAAAGG AGGATGCATG
    CTGCTATTGT CGCTGAAAAA AAAGCCTGAT GGTGGGTCTA GCGCTGAAGA GAGCAGCGCC
    CTGCAGAACG AGGAGACCGC TGGGGCATCA TCTGAGGAGT CGGAGGAACC TCACACGGTG
    CTAGTGGAGT TTTCTTCAGT GGTCGCCGAT ACTCAGGAAT ATATCATAGA GACTGCTGCT
    GAAGACTCCG AGGCCAGTGA AGCTGCTGAG CTCATCCAGG ATTCCGGATC CCAAGAGGTT
    GACGGCCACA TCTATTCAGT GGTTCAGCAG CTCGTAAGCG ACGCAAGCCC GGGCCAACAG
    ATCATTGTCC GCAACGTGTC TATCGACGGC GAGGAGAGGG TAGAGGAAGT GCCCACTACT
    GACACCATTG CTATAGCGAC TCCTGAACTG CTCACGGAGC AAGTTGCCTT GACTTTGGCC
    TCTGCCATTG AGGACGGTGC TGTAGTGGCC AAGGAGGAGC CCATGGAGGA GCAACAAATT
    CCAATCCCAG AGGAAGTGGA GATGATGGAG CACGCAGGGG AATTTGTCAT TGAGTCCCAT
    GACGGAGATC TAGAAGTTCA GACAGTCATT GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825833.1
    CDS107..2422
    Translation

    Target ORF information:

    RefSeq Version XM_012970379.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGGTAGT GCCCTCTATA GAAGAGACTG AAACCATCTC CCAAGTCATT
    GTGGAATCCA CAGAAGATGT CAGTAATGAT CCTGATGAAG AAGTGGAGGA TATTAAGGAC
    ATCCTCAGTG AGGATAGCGC TCTAGGTGGG CAAAGAGATG TGGAGGAGTT GGAAGTTGAA
    TGGGATGAGA GTCGAGGGGA TCCGGATCAA GATGATTATG ATAATTTGGG CCTAGTGACA
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC
    GCCAATATAT TGAGAGCCCA CATGATCACC CACAGCACAA AAAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA AAAGCTAGTC
    CCTACCAAAG ATGCCAACAG CAAGGTGCCG GGCGCTTCCT GCCAACTTGC CAGCGATACC
    AAGGACTCTT CCCAAGTGAT CCACCTTTTG ACAGATGCCA ATGGTAACAT TTTGCAGGAA
    GTTCACATCC AGGTTCAAGC ACATGACAGT GAACCAGTAT CACTTGTGCC AGAGGCTGCG
    GCTGCTGAGC GGTGTGCAGT AGATGGGGAG CTGAGCAGTG ACAGTCCCCT CCATCATATG
    AACAGCTCCA GTGTTGTGAT TGAGACTGTG TCCATGCAGG ATGTGGAGAG CGAGGAAATA
    ATCATAGCCT CAGCAGATGA GCCACAAAGT GGCGAAATGG ACGAGTTTGT TAACGGCCAG
    AAAACGATAG ATGGGACAGC AGACAGCGAT GATCCAGCTC TGAAATGTCC AAAGTGTAAT
    ACCATTTGCA TAAGTTCCAA TGCTTTGAGT GTCCACTTGA AAGGGCATGA AGGGTGCATG
    CTGTACTCGT GTGAAGTGTG CGGCAGGGAG TTCCTGAAGC GCCACCTGCT CAGGAAGCAC
    CAGGAGTGCC ATTTCAGCGA GCGCAAATAC AAGTGCGGAG AGTGCGGCAA AATGTACAAG
    ACTGTCGCGC ACGTCAAGGG CCACATGAGA GTGCACTCGG ATGATCGGCC GTACCCTTGT
    CCCAAGTGTG GCAAGAGATA CAAAACCAAG AATGCACAGC AGGTCCACTC TCGCACCCAC
    CTGGATGAGA AGCCATATGT CTGCCAGTAC TGCCCCAACA GCTTCCGGGA GAAGGGCTCT
    CTTGTCCGGC ACATCAGGCA CCACACTGGT GAGAAGCCCT TCAAGTGCCA CAAATGTGGC
    CGAGGCTTTG CCGAGCATGG AACTCTAAAT CGACATTTAA AAACCAAAGG AGGATGCATG
    CTGCTATTGT CGCTGAAAAA AAAGCCTGAT GGTGGGTCTA GCGCTGAAGA GAGCAGCGCC
    CTGCAGAACG AGGAGACCGC TGGGGCATCA TCTGAGGAGT CGGAGGAACC TCACACGGTG
    CTAGTGGAGT TTTCTTCAGT GGTCGCCGAT ACTCAGGAAT ATATCATAGA GACTGCTGCT
    GAAGACTCCG AGGCCAGTGA AGCTGCTGAG CTCATCCAGG ATTCCGGATC CCAAGAGGTT
    GACGGCCACA TCTATTCAGT GGTTCAGCAG CTCGTAAGCG ACGCAAGCCC GGGCCAACAG
    ATCATTGTCC GCAACGTGTC TATCGACGGC GAGGAGAGGG TAGAGGAAGT GCCCACTACT
    GACACCATTG CTATAGCGAC TCCTGAACTG CTCACGGAGC AAGTTGCCTT GACTTTGGCC
    TCTGCCATTG AGGACGGTGC TGTAGTGGCC AAGGAGGAGC CCATGGAGGA GCAACAAATT
    CCAATCCCAG AGGAAGTGGA GATGATGGAG CACGCAGGGG AATTTGTCAT TGAGTCCCAT
    GACGGAGATC TAGAAGTTCA GACAGTCATT GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa27353
    Clone ID Related Accession (Same CDS sequence) XM_012970380.2
    Accession Version XM_012970380.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGATA
    GATGGGACAG CAGACAGCGA TGATCCAGCT CTGAAATGTC CAAAGTGTAA TACCATTTGC
    ATAAGTTCCA ATGCTTTGAG TGTCCACTTG AAAGGGCATG AAGGGTGCAT GCTGTACTCG
    TGTGAAGTGT GCGGCAGGGA GTTCCTGAAG CGCCACCTGC TCAGGAAGCA CCAGGAGTGC
    CATTTCAGCG AGCGCAAATA CAAGTGCGGA GAGTGCGGCA AAATGTACAA GACTGTCGCG
    CACGTCAAGG GCCACATGAG AGTGCACTCG GATGATCGGC CGTACCCTTG TCCCAAGTGT
    GGCAAGAGAT ACAAAACCAA GAATGCACAG CAGGTCCACT CTCGCACCCA CCTGGATGAG
    AAGCCATATG TCTGCCAGTA CTGCCCCAAC AGCTTCCGGG AGAAGGGCTC TCTTGTCCGG
    CACATCAGGC ACCACACTGG TGAGAAGCCC TTCAAGTGCC ACAAATGTGG CCGAGGCTTT
    GCCGAGCATG GAACTCTAAA TCGACATTTA AAAACCAAAG GAGGATGCAT GCTGCTATTG
    TCGCTGAAAA AAAAGCCTGA TGGTGGGTCT AGCGCTGAAG AGAGCAGCGC CCTGCAGAAC
    GAGGAGACCG CTGGGGCATC ATCTGAGGAG TCGGAGGAAC CTCACACGGT GCTAGTGGAG
    TTTTCTTCAG TGGTCGCCGA TACTCAGGAA TATATCATAG AGACTGCTGC TGAAGACTCC
    GAGGCCAGTG AAGCTGCTGA GCTCATCCAG GATTCCGGAT CCCAAGAGGT TGACGGCCAC
    ATCTATTCAG TGGTTCAGCA GCTCGTAAGC GACGCAAGCC CGGGCCAACA GATCATTGTC
    CGCAACGTGT CTATCGACGG CGAGGAGAGG GTAGAGGAAG TGCCCACTAC TGACACCATT
    GCTATAGCGA CTCCTGAACT GCTCACGGAG CAAGTTGCCT TGACTTTGGC CTCTGCCATT
    GAGGACGGTG CTGTAGTGGC CAAGGAGGAG CCCATGGAGG AGCAACAAAT TCCAATCCCA
    GAGGAAGTGG AGATGATGGA GCACGCAGGG GAATTTGTCA TTGAGTCCCA TGACGGAGAT
    CTAGAAGTTC AGACAGTCAT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825834.1
    CDS106..2232
    Translation

    Target ORF information:

    RefSeq Version XM_012970380.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970380.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACAATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAATA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAAAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGATA
    GATGGGACAG CAGACAGCGA TGATCCAGCT CTGAAATGTC CAAAGTGTAA TACCATTTGC
    ATAAGTTCCA ATGCTTTGAG TGTCCACTTG AAAGGGCATG AAGGGTGCAT GCTGTACTCG
    TGTGAAGTGT GCGGCAGGGA GTTCCTGAAG CGCCACCTGC TCAGGAAGCA CCAGGAGTGC
    CATTTCAGCG AGCGCAAATA CAAGTGCGGA GAGTGCGGCA AAATGTACAA GACTGTCGCG
    CACGTCAAGG GCCACATGAG AGTGCACTCG GATGATCGGC CGTACCCTTG TCCCAAGTGT
    GGCAAGAGAT ACAAAACCAA GAATGCACAG CAGGTCCACT CTCGCACCCA CCTGGATGAG
    AAGCCATATG TCTGCCAGTA CTGCCCCAAC AGCTTCCGGG AGAAGGGCTC TCTTGTCCGG
    CACATCAGGC ACCACACTGG TGAGAAGCCC TTCAAGTGCC ACAAATGTGG CCGAGGCTTT
    GCCGAGCATG GAACTCTAAA TCGACATTTA AAAACCAAAG GAGGATGCAT GCTGCTATTG
    TCGCTGAAAA AAAAGCCTGA TGGTGGGTCT AGCGCTGAAG AGAGCAGCGC CCTGCAGAAC
    GAGGAGACCG CTGGGGCATC ATCTGAGGAG TCGGAGGAAC CTCACACGGT GCTAGTGGAG
    TTTTCTTCAG TGGTCGCCGA TACTCAGGAA TATATCATAG AGACTGCTGC TGAAGACTCC
    GAGGCCAGTG AAGCTGCTGA GCTCATCCAG GATTCCGGAT CCCAAGAGGT TGACGGCCAC
    ATCTATTCAG TGGTTCAGCA GCTCGTAAGC GACGCAAGCC CGGGCCAACA GATCATTGTC
    CGCAACGTGT CTATCGACGG CGAGGAGAGG GTAGAGGAAG TGCCCACTAC TGACACCATT
    GCTATAGCGA CTCCTGAACT GCTCACGGAG CAAGTTGCCT TGACTTTGGC CTCTGCCATT
    GAGGACGGTG CTGTAGTGGC CAAGGAGGAG CCCATGGAGG AGCAACAAAT TCCAATCCCA
    GAGGAAGTGG AGATGATGGA GCACGCAGGG GAATTTGTCA TTGAGTCCCA TGACGGAGAT
    CTAGAAGTTC AGACAGTCAT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66005
    Clone ID Related Accession (Same CDS sequence) XM_012970378.3
    Accession Version XM_012970378.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGGCT
    GCGGCTGCTG AGCGGTGTGC AGTAGATGGG GAGCTGAGCA GTGACAGTCC CCTCCATCAT
    ATGAACAGCT CCAGTGTTGT GATTGAGACT GTGTCCATGC AGGATGTGGA GAGCGAGGAA
    ATAATCATAG CCTCAGCAGA TGAGCCACAA AGTGGCGAAA TGGACGAGTT TGTTAACGGC
    CAGAAAACGA TAGATGGGAC AGCAGACAGC GATGATCCAG CTCTGAAATG TCCAAAGTGT
    AATACCATTT GCATAAGTTC CAATGCTTTG AGTGTCCACT TGAAAGGGCA TGAAGGGTGC
    ATGCTGTACT CGTGTGAAGT GTGCGGCAGG GAGTTCCTGA AGCGCCACCT GCTCAGGAAG
    CACCAGGAGT GCCATTTCAG CGAGCGCAAA TACAAGTGCG GAGAGTGCGG CAAAATGTAC
    AAGACTGTCG CGCACGTCAA GGGCCACATG AGAGTGCACT CGGATGATCG GCCGTACCCT
    TGTCCCAAGT GTGGCAAGAG ATACAAAACC AAGAATGCAC AGCAGGTCCA CTCTCGCACC
    CACCTGGATG AGAAGCCATA TGTCTGCCAG TACTGCCCCA ACAGCTTCCG GGAGAAGGGC
    TCTCTTGTCC GGCACATCAG GCACCACACT GGTGAGAAGC CCTTCAAGTG CCACAAATGT
    GGCCGAGGCT TTGCCGAGCA TGGAACTCTA AATCGACATT TAAAAACCAA AGGAGGATGC
    ATGCTGCTAT TGTCGCTGAA AAAAAAGCCT GATGGTGGGT CTAGCGCTGA AGAGAGCAGC
    GCCCTGCAGA ACGAGGAGAC CGCTGGGGCA TCATCTGAGG AGTCGGAGGA ACCTCACACG
    GTGCTAGTGG AGTTTTCTTC AGTGGTCGCC GATACTCAGG AATATATCAT AGAGACTGCT
    GCTGAAGACT CCGAGGCCAG TGAAGCTGCT GAGCTCATCC AGGATTCCGG ATCCCAAGAG
    GTTGACGGCC ACATCTATTC AGTGGTTCAG CAGCTCGTAA GCGACGCAAG CCCGGGCCAA
    CAGATCATTG TCCGCAACGT GTCTATCGAC GGCGAGGAGA GGGTAGAGGA AGTGCCCACT
    ACTGACACCA TTGCTATAGC GACTCCTGAA CTGCTCACGG AGCAAGTTGC CTTGACTTTG
    GCCTCTGCCA TTGAGGACGG TGCTGTAGTG GCCAAGGAGG AGCCCATGGA GGAGCAACAA
    ATTCCAATCC CAGAGGAAGT GGAGATGATG GAGCACGCAG GGGAATTTGT CATTGAGTCC
    CATGACGGAG ATCTAGAAGT TCAGACAGTC ATTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825832.1
    CDS109..2427
    Translation

    Target ORF information:

    RefSeq Version XM_012970378.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970378.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGGCT
    GCGGCTGCTG AGCGGTGTGC AGTAGATGGG GAGCTGAGCA GTGACAGTCC CCTCCATCAT
    ATGAACAGCT CCAGTGTTGT GATTGAGACT GTGTCCATGC AGGATGTGGA GAGCGAGGAA
    ATAATCATAG CCTCAGCAGA TGAGCCACAA AGTGGCGAAA TGGACGAGTT TGTTAACGGC
    CAGAAAACGA TAGATGGGAC AGCAGACAGC GATGATCCAG CTCTGAAATG TCCAAAGTGT
    AATACCATTT GCATAAGTTC CAATGCTTTG AGTGTCCACT TGAAAGGGCA TGAAGGGTGC
    ATGCTGTACT CGTGTGAAGT GTGCGGCAGG GAGTTCCTGA AGCGCCACCT GCTCAGGAAG
    CACCAGGAGT GCCATTTCAG CGAGCGCAAA TACAAGTGCG GAGAGTGCGG CAAAATGTAC
    AAGACTGTCG CGCACGTCAA GGGCCACATG AGAGTGCACT CGGATGATCG GCCGTACCCT
    TGTCCCAAGT GTGGCAAGAG ATACAAAACC AAGAATGCAC AGCAGGTCCA CTCTCGCACC
    CACCTGGATG AGAAGCCATA TGTCTGCCAG TACTGCCCCA ACAGCTTCCG GGAGAAGGGC
    TCTCTTGTCC GGCACATCAG GCACCACACT GGTGAGAAGC CCTTCAAGTG CCACAAATGT
    GGCCGAGGCT TTGCCGAGCA TGGAACTCTA AATCGACATT TAAAAACCAA AGGAGGATGC
    ATGCTGCTAT TGTCGCTGAA AAAAAAGCCT GATGGTGGGT CTAGCGCTGA AGAGAGCAGC
    GCCCTGCAGA ACGAGGAGAC CGCTGGGGCA TCATCTGAGG AGTCGGAGGA ACCTCACACG
    GTGCTAGTGG AGTTTTCTTC AGTGGTCGCC GATACTCAGG AATATATCAT AGAGACTGCT
    GCTGAAGACT CCGAGGCCAG TGAAGCTGCT GAGCTCATCC AGGATTCCGG ATCCCAAGAG
    GTTGACGGCC ACATCTATTC AGTGGTTCAG CAGCTCGTAA GCGACGCAAG CCCGGGCCAA
    CAGATCATTG TCCGCAACGT GTCTATCGAC GGCGAGGAGA GGGTAGAGGA AGTGCCCACT
    ACTGACACCA TTGCTATAGC GACTCCTGAA CTGCTCACGG AGCAAGTTGC CTTGACTTTG
    GCCTCTGCCA TTGAGGACGG TGCTGTAGTG GCCAAGGAGG AGCCCATGGA GGAGCAACAA
    ATTCCAATCC CAGAGGAAGT GGAGATGATG GAGCACGCAG GGGAATTTGT CATTGAGTCC
    CATGACGGAG ATCTAGAAGT TCAGACAGTC ATTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66006
    Clone ID Related Accession (Same CDS sequence) XM_012970379.3
    Accession Version XM_012970379.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGGTAGT GCCCTCTATA GAAGAGACTG AAACCATCTC CCAAGTCATT
    GTGGAATCCA CAGAAGATGT CAGTAATGAT CCTGATGAAG AAGTGGAGGA TATTAAGGAC
    ATCCTCAGTG AGGATAGCGC TCTAGGTGGG CAAAGAGATG TGGAGGAGTT GGAAGTTGAA
    TGGGATGAGA GTCGAGGGGA TCCGGATCAA GATGATTATG ATAATTTGGG CCTAGTGACG
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC
    GCCAACATAT TGAGAGCCCA CATGATCACC CACAGCACAA AGAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA AAAGCTAGTC
    CCTACCAAAG ATGCCAACAG CAAGGTGCCG GGCGCTTCCT GCCAACTTGC CAGCGATACC
    AAGGACTCTT CCCAAGTGAT CCACCTTTTG ACAGATGCCA ATGGTAACAT TTTGCAGGAA
    GTTCACATCC AGGTTCAAGC ACATGACAGT GAACCAGTAT CACTTGTGCC AGAGGCTGCG
    GCTGCTGAGC GGTGTGCAGT AGATGGGGAG CTGAGCAGTG ACAGTCCCCT CCATCATATG
    AACAGCTCCA GTGTTGTGAT TGAGACTGTG TCCATGCAGG ATGTGGAGAG CGAGGAAATA
    ATCATAGCCT CAGCAGATGA GCCACAAAGT GGCGAAATGG ACGAGTTTGT TAACGGCCAG
    AAAACGATAG ATGGGACAGC AGACAGCGAT GATCCAGCTC TGAAATGTCC AAAGTGTAAT
    ACCATTTGCA TAAGTTCCAA TGCTTTGAGT GTCCACTTGA AAGGGCATGA AGGGTGCATG
    CTGTACTCGT GTGAAGTGTG CGGCAGGGAG TTCCTGAAGC GCCACCTGCT CAGGAAGCAC
    CAGGAGTGCC ATTTCAGCGA GCGCAAATAC AAGTGCGGAG AGTGCGGCAA AATGTACAAG
    ACTGTCGCGC ACGTCAAGGG CCACATGAGA GTGCACTCGG ATGATCGGCC GTACCCTTGT
    CCCAAGTGTG GCAAGAGATA CAAAACCAAG AATGCACAGC AGGTCCACTC TCGCACCCAC
    CTGGATGAGA AGCCATATGT CTGCCAGTAC TGCCCCAACA GCTTCCGGGA GAAGGGCTCT
    CTTGTCCGGC ACATCAGGCA CCACACTGGT GAGAAGCCCT TCAAGTGCCA CAAATGTGGC
    CGAGGCTTTG CCGAGCATGG AACTCTAAAT CGACATTTAA AAACCAAAGG AGGATGCATG
    CTGCTATTGT CGCTGAAAAA AAAGCCTGAT GGTGGGTCTA GCGCTGAAGA GAGCAGCGCC
    CTGCAGAACG AGGAGACCGC TGGGGCATCA TCTGAGGAGT CGGAGGAACC TCACACGGTG
    CTAGTGGAGT TTTCTTCAGT GGTCGCCGAT ACTCAGGAAT ATATCATAGA GACTGCTGCT
    GAAGACTCCG AGGCCAGTGA AGCTGCTGAG CTCATCCAGG ATTCCGGATC CCAAGAGGTT
    GACGGCCACA TCTATTCAGT GGTTCAGCAG CTCGTAAGCG ACGCAAGCCC GGGCCAACAG
    ATCATTGTCC GCAACGTGTC TATCGACGGC GAGGAGAGGG TAGAGGAAGT GCCCACTACT
    GACACCATTG CTATAGCGAC TCCTGAACTG CTCACGGAGC AAGTTGCCTT GACTTTGGCC
    TCTGCCATTG AGGACGGTGC TGTAGTGGCC AAGGAGGAGC CCATGGAGGA GCAACAAATT
    CCAATCCCAG AGGAAGTGGA GATGATGGAG CACGCAGGGG AATTTGTCAT TGAGTCCCAT
    GACGGAGATC TAGAAGTTCA GACAGTCATT GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825833.1
    CDS109..2424
    Translation

    Target ORF information:

    RefSeq Version XM_012970379.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970379.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGGTAGT GCCCTCTATA GAAGAGACTG AAACCATCTC CCAAGTCATT
    GTGGAATCCA CAGAAGATGT CAGTAATGAT CCTGATGAAG AAGTGGAGGA TATTAAGGAC
    ATCCTCAGTG AGGATAGCGC TCTAGGTGGG CAAAGAGATG TGGAGGAGTT GGAAGTTGAA
    TGGGATGAGA GTCGAGGGGA TCCGGATCAA GATGATTATG ATAATTTGGG CCTAGTGACG
    ATGGTTTTGA ATGAAAGAGG CCGCTATGTT TGCCAGCTGT GTGACAAGAC ATTTAAGACC
    GCCAACATAT TGAGAGCCCA CATGATCACC CACAGCACAA AGAAGGAGTT TGAGTGCAAG
    CTGTGCGGAA ACTCTTTCAG GACAAAGGGG TCCTTAATAC GGCATAACAG AAGGCATACC
    GATGAGCGCC CTTACGTCTG CTCAAAGTGC GGCAAGAGTT TTCGAGAGTC GGGGGCCTTG
    ACGCGGCACT TCAGGTCTCT CACTCCCTGC ACAGAGAAGA TCCGACTCAA CATGTGCAAG
    GAAATCATGA TTTCCAAGGA GGAATACGCT TTAGGGCTAC AAAAAACAGA AAAGCTAGTC
    CCTACCAAAG ATGCCAACAG CAAGGTGCCG GGCGCTTCCT GCCAACTTGC CAGCGATACC
    AAGGACTCTT CCCAAGTGAT CCACCTTTTG ACAGATGCCA ATGGTAACAT TTTGCAGGAA
    GTTCACATCC AGGTTCAAGC ACATGACAGT GAACCAGTAT CACTTGTGCC AGAGGCTGCG
    GCTGCTGAGC GGTGTGCAGT AGATGGGGAG CTGAGCAGTG ACAGTCCCCT CCATCATATG
    AACAGCTCCA GTGTTGTGAT TGAGACTGTG TCCATGCAGG ATGTGGAGAG CGAGGAAATA
    ATCATAGCCT CAGCAGATGA GCCACAAAGT GGCGAAATGG ACGAGTTTGT TAACGGCCAG
    AAAACGATAG ATGGGACAGC AGACAGCGAT GATCCAGCTC TGAAATGTCC AAAGTGTAAT
    ACCATTTGCA TAAGTTCCAA TGCTTTGAGT GTCCACTTGA AAGGGCATGA AGGGTGCATG
    CTGTACTCGT GTGAAGTGTG CGGCAGGGAG TTCCTGAAGC GCCACCTGCT CAGGAAGCAC
    CAGGAGTGCC ATTTCAGCGA GCGCAAATAC AAGTGCGGAG AGTGCGGCAA AATGTACAAG
    ACTGTCGCGC ACGTCAAGGG CCACATGAGA GTGCACTCGG ATGATCGGCC GTACCCTTGT
    CCCAAGTGTG GCAAGAGATA CAAAACCAAG AATGCACAGC AGGTCCACTC TCGCACCCAC
    CTGGATGAGA AGCCATATGT CTGCCAGTAC TGCCCCAACA GCTTCCGGGA GAAGGGCTCT
    CTTGTCCGGC ACATCAGGCA CCACACTGGT GAGAAGCCCT TCAAGTGCCA CAAATGTGGC
    CGAGGCTTTG CCGAGCATGG AACTCTAAAT CGACATTTAA AAACCAAAGG AGGATGCATG
    CTGCTATTGT CGCTGAAAAA AAAGCCTGAT GGTGGGTCTA GCGCTGAAGA GAGCAGCGCC
    CTGCAGAACG AGGAGACCGC TGGGGCATCA TCTGAGGAGT CGGAGGAACC TCACACGGTG
    CTAGTGGAGT TTTCTTCAGT GGTCGCCGAT ACTCAGGAAT ATATCATAGA GACTGCTGCT
    GAAGACTCCG AGGCCAGTGA AGCTGCTGAG CTCATCCAGG ATTCCGGATC CCAAGAGGTT
    GACGGCCACA TCTATTCAGT GGTTCAGCAG CTCGTAAGCG ACGCAAGCCC GGGCCAACAG
    ATCATTGTCC GCAACGTGTC TATCGACGGC GAGGAGAGGG TAGAGGAAGT GCCCACTACT
    GACACCATTG CTATAGCGAC TCCTGAACTG CTCACGGAGC AAGTTGCCTT GACTTTGGCC
    TCTGCCATTG AGGACGGTGC TGTAGTGGCC AAGGAGGAGC CCATGGAGGA GCAACAAATT
    CCAATCCCAG AGGAAGTGGA GATGATGGAG CACGCAGGGG AATTTGTCAT TGAGTCCCAT
    GACGGAGATC TAGAAGTTCA GACAGTCATT GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66007
    Clone ID Related Accession (Same CDS sequence) XM_012970380.3
    Accession Version XM_012970380.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGATA
    GATGGGACAG CAGACAGCGA TGATCCAGCT CTGAAATGTC CAAAGTGTAA TACCATTTGC
    ATAAGTTCCA ATGCTTTGAG TGTCCACTTG AAAGGGCATG AAGGGTGCAT GCTGTACTCG
    TGTGAAGTGT GCGGCAGGGA GTTCCTGAAG CGCCACCTGC TCAGGAAGCA CCAGGAGTGC
    CATTTCAGCG AGCGCAAATA CAAGTGCGGA GAGTGCGGCA AAATGTACAA GACTGTCGCG
    CACGTCAAGG GCCACATGAG AGTGCACTCG GATGATCGGC CGTACCCTTG TCCCAAGTGT
    GGCAAGAGAT ACAAAACCAA GAATGCACAG CAGGTCCACT CTCGCACCCA CCTGGATGAG
    AAGCCATATG TCTGCCAGTA CTGCCCCAAC AGCTTCCGGG AGAAGGGCTC TCTTGTCCGG
    CACATCAGGC ACCACACTGG TGAGAAGCCC TTCAAGTGCC ACAAATGTGG CCGAGGCTTT
    GCCGAGCATG GAACTCTAAA TCGACATTTA AAAACCAAAG GAGGATGCAT GCTGCTATTG
    TCGCTGAAAA AAAAGCCTGA TGGTGGGTCT AGCGCTGAAG AGAGCAGCGC CCTGCAGAAC
    GAGGAGACCG CTGGGGCATC ATCTGAGGAG TCGGAGGAAC CTCACACGGT GCTAGTGGAG
    TTTTCTTCAG TGGTCGCCGA TACTCAGGAA TATATCATAG AGACTGCTGC TGAAGACTCC
    GAGGCCAGTG AAGCTGCTGA GCTCATCCAG GATTCCGGAT CCCAAGAGGT TGACGGCCAC
    ATCTATTCAG TGGTTCAGCA GCTCGTAAGC GACGCAAGCC CGGGCCAACA GATCATTGTC
    CGCAACGTGT CTATCGACGG CGAGGAGAGG GTAGAGGAAG TGCCCACTAC TGACACCATT
    GCTATAGCGA CTCCTGAACT GCTCACGGAG CAAGTTGCCT TGACTTTGGC CTCTGCCATT
    GAGGACGGTG CTGTAGTGGC CAAGGAGGAG CCCATGGAGG AGCAACAAAT TCCAATCCCA
    GAGGAAGTGG AGATGATGGA GCACGCAGGG GAATTTGTCA TTGAGTCCCA TGACGGAGAT
    CTAGAAGTTC AGACAGTCAT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825834.1
    CDS108..2234
    Translation

    Target ORF information:

    RefSeq Version XM_012970380.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970380.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGAAAAGCTA
    GTCCCTACCA AAGATGCCAA CAGCAAGGTG CCGGGCGCTT CCTGCCAACT TGCCAGCGAT
    ACCAAGGACT CTTCCCAAGT GATCCACCTT TTGACAGATG CCAATGGTAA CATTTTGCAG
    GAAGTTCACA TCCAGGTTCA AGCACATGAC AGTGAACCAG TATCACTTGT GCCAGAGATA
    GATGGGACAG CAGACAGCGA TGATCCAGCT CTGAAATGTC CAAAGTGTAA TACCATTTGC
    ATAAGTTCCA ATGCTTTGAG TGTCCACTTG AAAGGGCATG AAGGGTGCAT GCTGTACTCG
    TGTGAAGTGT GCGGCAGGGA GTTCCTGAAG CGCCACCTGC TCAGGAAGCA CCAGGAGTGC
    CATTTCAGCG AGCGCAAATA CAAGTGCGGA GAGTGCGGCA AAATGTACAA GACTGTCGCG
    CACGTCAAGG GCCACATGAG AGTGCACTCG GATGATCGGC CGTACCCTTG TCCCAAGTGT
    GGCAAGAGAT ACAAAACCAA GAATGCACAG CAGGTCCACT CTCGCACCCA CCTGGATGAG
    AAGCCATATG TCTGCCAGTA CTGCCCCAAC AGCTTCCGGG AGAAGGGCTC TCTTGTCCGG
    CACATCAGGC ACCACACTGG TGAGAAGCCC TTCAAGTGCC ACAAATGTGG CCGAGGCTTT
    GCCGAGCATG GAACTCTAAA TCGACATTTA AAAACCAAAG GAGGATGCAT GCTGCTATTG
    TCGCTGAAAA AAAAGCCTGA TGGTGGGTCT AGCGCTGAAG AGAGCAGCGC CCTGCAGAAC
    GAGGAGACCG CTGGGGCATC ATCTGAGGAG TCGGAGGAAC CTCACACGGT GCTAGTGGAG
    TTTTCTTCAG TGGTCGCCGA TACTCAGGAA TATATCATAG AGACTGCTGC TGAAGACTCC
    GAGGCCAGTG AAGCTGCTGA GCTCATCCAG GATTCCGGAT CCCAAGAGGT TGACGGCCAC
    ATCTATTCAG TGGTTCAGCA GCTCGTAAGC GACGCAAGCC CGGGCCAACA GATCATTGTC
    CGCAACGTGT CTATCGACGG CGAGGAGAGG GTAGAGGAAG TGCCCACTAC TGACACCATT
    GCTATAGCGA CTCCTGAACT GCTCACGGAG CAAGTTGCCT TGACTTTGGC CTCTGCCATT
    GAGGACGGTG CTGTAGTGGC CAAGGAGGAG CCCATGGAGG AGCAACAAAT TCCAATCCCA
    GAGGAAGTGG AGATGATGGA GCACGCAGGG GAATTTGTCA TTGAGTCCCA TGACGGAGAT
    CTAGAAGTTC AGACAGTCAT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66008
    Clone ID Related Accession (Same CDS sequence) XM_012970381.3
    Accession Version XM_012970381.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product transcription factor E4F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGATGGGACA
    GCAGACAGCG ATGATCCAGC TCTGAAATGT CCAAAGTGTA ATACCATTTG CATAAGTTCC
    AATGCTTTGA GTGTCCACTT GAAAGGGCAT GAAGGGTGCA TGCTGTACTC GTGTGAAGTG
    TGCGGCAGGG AGTTCCTGAA GCGCCACCTG CTCAGGAAGC ACCAGGAGTG CCATTTCAGC
    GAGCGCAAAT ACAAGTGCGG AGAGTGCGGC AAAATGTACA AGACTGTCGC GCACGTCAAG
    GGCCACATGA GAGTGCACTC GGATGATCGG CCGTACCCTT GTCCCAAGTG TGGCAAGAGA
    TACAAAACCA AGAATGCACA GCAGGTCCAC TCTCGCACCC ACCTGGATGA GAAGCCATAT
    GTCTGCCAGT ACTGCCCCAA CAGCTTCCGG GAGAAGGGCT CTCTTGTCCG GCACATCAGG
    CACCACACTG GTGAGAAGCC CTTCAAGTGC CACAAATGTG GCCGAGGCTT TGCCGAGCAT
    GGAACTCTAA ATCGACATTT AAAAACCAAA GGAGGATGCA TGCTGCTATT GTCGCTGAAA
    AAAAAGCCTG ATGGTGGGTC TAGCGCTGAA GAGAGCAGCG CCCTGCAGAA CGAGGAGACC
    GCTGGGGCAT CATCTGAGGA GTCGGAGGAA CCTCACACGG TGCTAGTGGA GTTTTCTTCA
    GTGGTCGCCG ATACTCAGGA ATATATCATA GAGACTGCTG CTGAAGACTC CGAGGCCAGT
    GAAGCTGCTG AGCTCATCCA GGATTCCGGA TCCCAAGAGG TTGACGGCCA CATCTATTCA
    GTGGTTCAGC AGCTCGTAAG CGACGCAAGC CCGGGCCAAC AGATCATTGT CCGCAACGTG
    TCTATCGACG GCGAGGAGAG GGTAGAGGAA GTGCCCACTA CTGACACCAT TGCTATAGCG
    ACTCCTGAAC TGCTCACGGA GCAAGTTGCC TTGACTTTGG CCTCTGCCAT TGAGGACGGT
    GCTGTAGTGG CCAAGGAGGA GCCCATGGAG GAGCAACAAA TTCCAATCCC AGAGGAAGTG
    GAGATGATGG AGCACGCAGG GGAATTTGTC ATTGAGTCCC ATGACGGAGA TCTAGAAGTT
    CAGACAGTCA TTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825835.1
    CDS107..2044
    Translation

    Target ORF information:

    RefSeq Version XM_012970381.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis E4F transcription factor 1 (e4f1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970381.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCTCAG AGCTCGGTGC GGGGGCAGGG GAGGCTGCTA GTTCAGCCGG CGCCCTCGTC 
    AGCCTGGAAG TGCCTGGGAC TCCGCTTATT ACTTGTTTCA AGGAGGAAGA TGAAGACGAT
    GTGCACAAAT GCGGCCGCTG TCAGGCTGAA TTCACGAGCC TGGAAGAGTT TGTCCACCAC
    AAGATACAAA AGATTTGCTT GAGAGCCCCG GATCCCAACC TGGTGTCCAC CGGTGCGGCA
    CTGAACGGCC AGGAGCAGGT AGTGCCCTCT ATAGAAGAGA CTGAAACCAT CTCCCAAGTC
    ATTGTGGAAT CCACAGAAGA TGTCAGTAAT GATCCTGATG AAGAAGTGGA GGATATTAAG
    GACATCCTCA GTGAGGATAG CGCTCTAGGT GGGCAAAGAG ATGTGGAGGA GTTGGAAGTT
    GAATGGGATG AGAGTCGAGG GGATCCGGAT CAAGATGATT ATGATAATTT GGGCCTAGTG
    ACGATGGTTT TGAATGAAAG AGGCCGCTAT GTTTGCCAGC TGTGTGACAA GACATTTAAG
    ACCGCCAACA TATTGAGAGC CCACATGATC ACCCACAGCA CAAAGAAGGA GTTTGAGTGC
    AAGCTGTGCG GAAACTCTTT CAGGACAAAG GGGTCCTTAA TACGGCATAA CAGAAGGCAT
    ACCGATGAGC GCCCTTACGT CTGCTCAAAG TGCGGCAAGA GTTTTCGAGA GTCGGGGGCC
    TTGACGCGGC ACTTCAGGTC TCTCACTCCC TGCACAGAGA AGATCCGACT CAACATGTGC
    AAGGAAATCA TGATTTCCAA GGAGGAATAC GCTTTAGGGC TACAAAAAAC AGATGGGACA
    GCAGACAGCG ATGATCCAGC TCTGAAATGT CCAAAGTGTA ATACCATTTG CATAAGTTCC
    AATGCTTTGA GTGTCCACTT GAAAGGGCAT GAAGGGTGCA TGCTGTACTC GTGTGAAGTG
    TGCGGCAGGG AGTTCCTGAA GCGCCACCTG CTCAGGAAGC ACCAGGAGTG CCATTTCAGC
    GAGCGCAAAT ACAAGTGCGG AGAGTGCGGC AAAATGTACA AGACTGTCGC GCACGTCAAG
    GGCCACATGA GAGTGCACTC GGATGATCGG CCGTACCCTT GTCCCAAGTG TGGCAAGAGA
    TACAAAACCA AGAATGCACA GCAGGTCCAC TCTCGCACCC ACCTGGATGA GAAGCCATAT
    GTCTGCCAGT ACTGCCCCAA CAGCTTCCGG GAGAAGGGCT CTCTTGTCCG GCACATCAGG
    CACCACACTG GTGAGAAGCC CTTCAAGTGC CACAAATGTG GCCGAGGCTT TGCCGAGCAT
    GGAACTCTAA ATCGACATTT AAAAACCAAA GGAGGATGCA TGCTGCTATT GTCGCTGAAA
    AAAAAGCCTG ATGGTGGGTC TAGCGCTGAA GAGAGCAGCG CCCTGCAGAA CGAGGAGACC
    GCTGGGGCAT CATCTGAGGA GTCGGAGGAA CCTCACACGG TGCTAGTGGA GTTTTCTTCA
    GTGGTCGCCG ATACTCAGGA ATATATCATA GAGACTGCTG CTGAAGACTC CGAGGCCAGT
    GAAGCTGCTG AGCTCATCCA GGATTCCGGA TCCCAAGAGG TTGACGGCCA CATCTATTCA
    GTGGTTCAGC AGCTCGTAAG CGACGCAAGC CCGGGCCAAC AGATCATTGT CCGCAACGTG
    TCTATCGACG GCGAGGAGAG GGTAGAGGAA GTGCCCACTA CTGACACCAT TGCTATAGCG
    ACTCCTGAAC TGCTCACGGA GCAAGTTGCC TTGACTTTGG CCTCTGCCAT TGAGGACGGT
    GCTGTAGTGG CCAAGGAGGA GCCCATGGAG GAGCAACAAA TTCCAATCCC AGAGGAAGTG
    GAGATGATGG AGCACGCAGG GGAATTTGTC ATTGAGTCCC ATGACGGAGA TCTAGAAGTT
    CAGACAGTCA TTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.