arhgef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef2
Entrez Gene ID	407848
Full Name	Rho/Rac guanine nucleotide exchange factor 2
Synonyms	XLfc
General protein information	Preferred Names Rho/Rac guanine nucleotide exchange factor 2 Names rho guanine nucleotide exchange factor 2 Rho/Rac guanine nucleotide exchange factor (GEF) 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031890301.1	XP_031746161.1	rho guanine nucleotide exchange factor 2 isoform X1
XM_031890302.1	XP_031746162.1	rho guanine nucleotide exchange factor 2 isoform X1
XM_031890303.1	XP_031746163.1	rho guanine nucleotide exchange factor 2 isoform X2
NM_001001456.1	NP_001001456.1	rho guanine nucleotide exchange factor 2
XM_031890300.1	XP_031746160.1	rho guanine nucleotide exchange factor 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGEF2	XP_003308546.1
	ARHGEF2	XP_002801891.1
Bos taurus (cattle)	ARHGEF2	NP_001092351.2
Rattus norvegicus (Norway rat)	Arhgef2	NP_001012079.1
Danio rerio (zebrafish)	arhgef1a	NP_001075100.1
Homo sapiens (human)	ARHGEF2	NP_001155855.1
Canis lupus familiaris (dog)	ARHGEF2	NP_001002995.1
Danio rerio (zebrafish)	arhgef2	XP_689770.5
Xenopus tropicalis (tropical clawed frog)	arhgef2	NP_001001456.1
Mus musculus (house mouse)	Arhgef2	NP_032513.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following arhgef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03027	NM_001001456.1 Latest version!	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa63576	XM_031890301.1 Latest version!	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63576	XM_031890300.1 Latest version!	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63576	XM_031890302.1 Latest version!	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63577	XM_031890303.1 Latest version!	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef2 ( NM_001001456.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001001456.1 Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), mRNA.

Price & Availability↑

CloneID	OXa03027
Clone ID Related Accession (Same CDS sequence)	NM_001001456.1
Accession Version	NM_001001456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066783.1. ##Evidence-Data-START## Transcript exon combination :: BC066783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00861986, SAMN00861988 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGGA TTGAGTCCTT TGGCAAAGCC CCGGCGGAGC GCACCAAGAA TAAAGAAAAA 
GAGAAGATGA AGGATTCCAA GGACCCCCGG TACACCAACG GGCACCTGTT CACCACCATC 
ACCGTGTCTG GGACGACCAT GTGCTTTGTG TGCAACAAGA GTATAACCGC TAAAGAGGCG 
CTGATTTGCC CCACCTGTAA CGTTACCATA CACAACCGCT GCAAAGATGC CCTGCCCAGC 
TGCACCAAGG TCAAACAGAA ACAACAGAAA GCCGCTTTCC TGAAGAACAA TTCTGCCCTG 
CAGAACGTCT CGCTGAGGAA CAAAACCACG ACGCTTCGAG AGCGCCCCAA TTCGGCCATC 
TACCCCTCGG ACAGCCTGCG CCACTCTATC CTGGGCTCCC GCCGGGGCCG GGCGTCTCTT 
TCTCTGTCTA AAAGTGTCTC CACCACCAAC ATTGCAGGGA ACCTGAACGA TGAGAGCCCA 
CTGGGAATCC GCCGGATCCT CTCCCAATCT ACCGACTCGC TCAATATGCG GAACCGGACT 
CTCTCCGTGG AGTCGCTCAT CGATGAAGGC GCCGAGGTCG TGTACAGTCA GCTGATGAGC 
GACTTCGCCA CCGAGGAGAA GGAATTCGAG GCGGATTCGT GGAGCCTGGC TGTGGATAAC 
AACTACCTCC AGCAGCACAA GAAAGATGTC ATGAAGCGGC AGGACGTGAT ATACGAGCTG 
ATCCAGACGG AGGTCCACCA CGTGCGCACC CTGAAGATCA TGACCAACAT ATTCCGCAAG 
GGGATGCTGG AGGATCTGCA GATGGACCCG GCGCTGGTCA ATAAGATGTT CCCCTGCGTG 
GACGAACTGA GCGACATTCA CATCCGATTC CTCATTCAGC TCCTGGAGAG GCGCAAAGAC 
TCGCTGGCGT CCGACAGCAA CAAGAACTTT GTCATCAACA AGCTGGGGGA TATCCTCATT 
AACCAGTTTT CAGGCACCAA TGCCGAGCGG ATGAAGAAAG CCTACACGGA ATTCGCCAGC 
CAGCACCATA AGGCCATGAA GCTGTACAAG GAGCTCTTCT CCCGGGATAA GAAGTTCCAG 
CAGATGATAC GGAAAATGAC CCGCTCCCCC CTGCTGCGCA GGCACGGCGT CCAGGAATGC 
ATCCTGCTAG TCACCCAGCG CATCACCAAG TACCCCGTGC TCCTTGACCG CATTCTGCAG 
AACTCTAAGG GCGATGAGGA GGAGTACCTA GACCTGGCCG AATCCTTGCG GATGGTGAAG 
GAGCTGATTA CGACGATTGA CCAAGACGTG CACAACCTGG AGAAGAGCTT ACGCTTGCAG 
GAGATCTACC AGCGAATAGA CAGCAAGTCT GTGGCACTAG TAGATGGGGA CCACAGCTTT 
AGCAAGGAGG AGCTGCTGCG CCGGAAACTC ATCCACGAGG GGTCGCTACT GTGGAAAACG 
GCTGCTGGGC GCTTCAAAGA TGTGATTATG CTGCTAATGA CAGACACCAT GATTCTGCTT 
CAGGAGAAAG ACCAGAAATA TTATTTCCCA ACGCTGGATA GATCTCCCGT CATCAGCCTG 
CAGAACCTGA TAGTGCGGGA CATTGCCAAC CAGGAGAAGG GCATGTTCCT AATCAGCGCC 
AAGCCCCCCG AGATGTACGA GGTACACGCC GCCTCCCGCG AGGAGCGCAA CACGTGGATG 
AAGCACATCG GCCAGTCGGT CAAAGTGTGC CCAAAGCGAG AGGAATTCCC GCTGATCGAG 
ACAGAAGTGG AGGCGAAACT ACGGAAGCTG AGAGAGGTGA TTCAGCAGAA GGACCGGGAG 
GTGTCGGAGC TCCTGGAGGA GAAGGTGACG CTCTTTTCCA AAGTGGTGCA GCTCCAGGCG 
GGGGAGAATT CCCAGGGGCC CGGCATCGGC GCCCCCCGCT GCCTCTTTCG AACCGACTCC 
ACCGACACCT ACAGGGGCGA GAAGCTGATC GGCGACACCA TCAAAGAAGT GGAAGCACTG 
AAAGACGTGA TCATTGGGGC TGGCAGCGAA CTGCCTCTTC TACGGACGGA ACAGAACCAC 
CCATTGGACA CTTCCAACAG CCAATCCAGA GATTCCACAT ATGGGGACAC AAACGCCGTC 
AATGGCTCCT GCGATGTTGC CAAGGAGGAC GATCCAAAGG ACCGGAACGG AAATCAGCTC 
CAAAACAAAA GCCCTCGTGA GGACGCCCTA CAGAGGATTG TCAATATCTA CAGCTTTCTA 
CATGGGCTGC AGGCGATGGT CATACAACAG GACACGCTAC AAGACGCGCC GCTCCAGGAC 
GTCGGCGACA AGAAGGAGCG CCTGTGCCGG ACCAACTCCC GCGATCCGAT TCTGATAGAA 
CCCAAGAGCG CAGACAAACA GACGGAGCTG ACTCTGCTGC AGCGCCAACA CACTCTGTTG 
CAGGAGGAGC TGCGCCGGTG CAAGCGGCAG TGCGACGACA GGGCGCAGGA GGCGGGGCTA 
CTGGAGACCC GGCTGAGGGA GAGCGAGCAG ATCCGGGGGA GGATGGAGCG GGAGTTGGAG 
GATGGGAAAA GGCAGCTGCT CCTCCTGAGA GAGAGAAACG ACGCGGGGCT GGAGGGGGCC 
AGGAATCACA AAGTGTTGGA TCCTCGTAGG CGGAGCTTGC CCGCGGGGGA CGCCCTGTAT 
CAGACCTTCA CTCCGCCCCC GGTTCACGTG GAGAGGAGGG TCCCGCTGGT TCTGGACGGC 
CCGGTCCCCA TCATTACTCT GCAGGACGAC TCGGACCTCA AAGACGACTT CAGCGATCTC 
ACCGAGCTGG AGAAACTGCC GGCCATGGCG GAAACGGAAT GGACGGAGGA GGAGGGCGGG 
GGGCCCCCAG CTTCTCCCTC CAGCGCACGA GAATTCCAGA GAATGCAGGA CATTCCAGAA 
GAGGCAGAGA GCGTTCAGGA GCTGAGAGAC GCGGAGCAGG GATCCTCCGA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001456.1
CDS	145..3081
Translation	MSRIESFGKAPAERTKNKEKEKMKDSKDPRYTNGHLFTTITVSGTTMCFVCNKSITAKEALICPTCNVTIHNRCKDALPSCTKVKQKQQKAAFLKNNSALQNVSLRNKTTTLRERPNSAIYPSDSLRHSILGSRRGRASLSLSKSVSTTNIAGNLNDESPLGIRRILSQSTDSLNMRNRTLSVESLIDEGAEVVYSQLMSDFATEEKEFEADSWSLAVDNNYLQQHKKDVMKRQDVIYELIQTEVHHVRTLKIMTNIFRKGMLEDLQMDPALVNKMFPCVDELSDIHIRFLIQLLERRKDSLASDSNKNFVINKLGDILINQFSGTNAERMKKAYTEFASQHHKAMKLYKELFSRDKKFQQMIRKMTRSPLLRRHGVQECILLVTQRITKYPVLLDRILQNSKGDEEEYLDLAESLRMVKELITTIDQDVHNLEKSLRLQEIYQRIDSKSVALVDGDHSFSKEELLRRKLIHEGSLLWKTAAGRFKDVIMLLMTDTMILLQEKDQKYYFPTLDRSPVISLQNLIVRDIANQEKGMFLISAKPPEMYEVHAASREERNTWMKHIGQSVKVCPKREEFPLIETEVEAKLRKLREVIQQKDREVSELLEEKVTLFSKVVQLQAGENSQGPGIGAPRCLFRTDSTDTYRGEKLIGDTIKEVEALKDVIIGAGSELPLLRTEQNHPLDTSNSQSRDSTYGDTNAVNGSCDVAKEDDPKDRNGNQLQNKSPREDALQRIVNIYSFLHGLQAMVIQQDTLQDAPLQDVGDKKERLCRTNSRDPILIEPKSADKQTELTLLQRQHTLLQEELRRCKRQCDDRAQEAGLLETRLRESEQIRGRMERELEDGKRQLLLLRERNDAGLEGARNHKVLDPRRRSLPAGDALYQTFTPPPVHVERRVPLVLDGPVPIITLQDDSDLKDDFSDLTELEKLPAMAETEWTEEEGGGPPASPSSAREFQRMQDIPEEAESVQELRDAEQGSSES

Target ORF information:

RefSeq Version	NM_001001456.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001456.1

ORF Insert Sequence:

ATGTCTCGGA TTGAGTCCTT TGGCAAAGCC CCGGCGGAGC GCACCAAGAA TAAAGAAAAA 
GAGAAGATGA AGGATTCCAA GGACCCCCGG TACACCAACG GGCACCTGTT CACCACCATC 
ACCGTGTCTG GGACGACCAT GTGCTTTGTG TGCAACAAGA GTATAACCGC TAAAGAGGCG 
CTGATTTGCC CCACCTGTAA CGTTACCATA CACAACCGCT GCAAAGATGC CCTGCCCAGC 
TGCACCAAGG TCAAACAGAA ACAACAGAAA GCCGCTTTCC TGAAGAACAA TTCTGCCCTG 
CAGAACGTCT CGCTGAGGAA CAAAACCACG ACGCTTCGAG AGCGCCCCAA TTCGGCCATC 
TACCCCTCGG ACAGCCTGCG CCACTCTATC CTGGGCTCCC GCCGGGGCCG GGCGTCTCTT 
TCTCTGTCTA AAAGTGTCTC CACCACCAAC ATTGCAGGGA ACCTGAACGA TGAGAGCCCA 
CTGGGAATCC GCCGGATCCT CTCCCAATCT ACCGACTCGC TCAATATGCG GAACCGGACT 
CTCTCCGTGG AGTCGCTCAT CGATGAAGGC GCCGAGGTCG TGTACAGTCA GCTGATGAGC 
GACTTCGCCA CCGAGGAGAA GGAATTCGAG GCGGATTCGT GGAGCCTGGC TGTGGATAAC 
AACTACCTCC AGCAGCACAA GAAAGATGTC ATGAAGCGGC AGGACGTGAT ATACGAGCTG 
ATCCAGACGG AGGTCCACCA CGTGCGCACC CTGAAGATCA TGACCAACAT ATTCCGCAAG 
GGGATGCTGG AGGATCTGCA GATGGACCCG GCGCTGGTCA ATAAGATGTT CCCCTGCGTG 
GACGAACTGA GCGACATTCA CATCCGATTC CTCATTCAGC TCCTGGAGAG GCGCAAAGAC 
TCGCTGGCGT CCGACAGCAA CAAGAACTTT GTCATCAACA AGCTGGGGGA TATCCTCATT 
AACCAGTTTT CAGGCACCAA TGCCGAGCGG ATGAAGAAAG CCTACACGGA ATTCGCCAGC 
CAGCACCATA AGGCCATGAA GCTGTACAAG GAGCTCTTCT CCCGGGATAA GAAGTTCCAG 
CAGATGATAC GGAAAATGAC CCGCTCCCCC CTGCTGCGCA GGCACGGCGT CCAGGAATGC 
ATCCTGCTAG TCACCCAGCG CATCACCAAG TACCCCGTGC TCCTTGACCG CATTCTGCAG 
AACTCTAAGG GCGATGAGGA GGAGTACCTA GACCTGGCCG AATCCTTGCG GATGGTGAAG 
GAGCTGATTA CGACGATTGA CCAAGACGTG CACAACCTGG AGAAGAGCTT ACGCTTGCAG 
GAGATCTACC AGCGAATAGA CAGCAAGTCT GTGGCACTAG TAGATGGGGA CCACAGCTTT 
AGCAAGGAGG AGCTGCTGCG CCGGAAACTC ATCCACGAGG GGTCGCTACT GTGGAAAACG 
GCTGCTGGGC GCTTCAAAGA TGTGATTATG CTGCTAATGA CAGACACCAT GATTCTGCTT 
CAGGAGAAAG ACCAGAAATA TTATTTCCCA ACGCTGGATA GATCTCCCGT CATCAGCCTG 
CAGAACCTGA TAGTGCGGGA CATTGCCAAC CAGGAGAAGG GCATGTTCCT AATCAGCGCC 
AAGCCCCCCG AGATGTACGA GGTACACGCC GCCTCCCGCG AGGAGCGCAA CACGTGGATG 
AAGCACATCG GCCAGTCGGT CAAAGTGTGC CCAAAGCGAG AGGAATTCCC GCTGATCGAG 
ACAGAAGTGG AGGCGAAACT ACGGAAGCTG AGAGAGGTGA TTCAGCAGAA GGACCGGGAG 
GTGTCGGAGC TCCTGGAGGA GAAGGTGACG CTCTTTTCCA AAGTGGTGCA GCTCCAGGCG 
GGGGAGAATT CCCAGGGGCC CGGCATCGGC GCCCCCCGCT GCCTCTTTCG AACCGACTCC 
ACCGACACCT ACAGGGGCGA GAAGCTGATC GGCGACACCA TCAAAGAAGT GGAAGCACTG 
AAAGACGTGA TCATTGGGGC TGGCAGCGAA CTGCCTCTTC TACGGACGGA ACAGAACCAC 
CCATTGGACA CTTCCAACAG CCAATCCAGA GATTCCACAT ATGGGGACAC AAACGCCGTC 
AATGGCTCCT GCGATGTTGC CAAGGAGGAC GATCCAAAGG ACCGGAACGG AAATCAGCTC 
CAAAACAAAA GCCCTCGTGA GGACGCCCTA CAGAGGATTG TCAATATCTA CAGCTTTCTA 
CATGGGCTGC AGGCGATGGT CATACAACAG GACACGCTAC AAGACGCGCC GCTCCAGGAC 
GTCGGCGACA AGAAGGAGCG CCTGTGCCGG ACCAACTCCC GCGATCCGAT TCTGATAGAA 
CCCAAGAGCG CAGACAAACA GACGGAGCTG ACTCTGCTGC AGCGCCAACA CACTCTGTTG 
CAGGAGGAGC TGCGCCGGTG CAAGCGGCAG TGCGACGACA GGGCGCAGGA GGCGGGGCTA 
CTGGAGACCC GGCTGAGGGA GAGCGAGCAG ATCCGGGGGA GGATGGAGCG GGAGTTGGAG 
GATGGGAAAA GGCAGCTGCT CCTCCTGAGA GAGAGAAACG ACGCGGGGCT GGAGGGGGCC 
AGGAATCACA AAGTGTTGGA TCCTCGTAGG CGGAGCTTGC CCGCGGGGGA CGCCCTGTAT 
CAGACCTTCA CTCCGCCCCC GGTTCACGTG GAGAGGAGGG TCCCGCTGGT TCTGGACGGC 
CCGGTCCCCA TCATTACTCT GCAGGACGAC TCGGACCTCA AAGACGACTT CAGCGATCTC 
ACCGAGCTGG AGAAACTGCC GGCCATGGCG GAAACGGAAT GGACGGAGGA GGAGGGCGGG 
GGGCCCCCAG CTTCTCCCTC CAGCGCACGA GAATTCCAGA GAATGCAGGA CATTCCAGAA 
GAGGCAGAGA GCGTTCAGGA GCTGAGAGAC GCGGAGCAGG GATCCTCCGA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef2 ( XM_031890301.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746161.1 Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63576
Clone ID Related Accession (Same CDS sequence)	XM_031890301.1 , XM_031890302.1 , XM_031890300.1
Accession Version	XM_031890301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746161.1
CDS	160..3741
Translation	MNDAEVFVTGGEAGPFMARVPKQRCLSALDPRMPSEEDLHNEELDGFIAVPLRRSCRLRSSMHTIDAFRRHSWEPGKRMQEMEADYEDLSQSLKGLDPEEIEGNMGNRRDPRRTPIISSTDELESWLSTRGEDQEVTQEEHAKKLQAYLFSQQYLYNPLSKSVSMSGIDRPFCYPDDVTLYSQGRHLSNGALSCGDFDALETDEKENREKEESPLGRTLSFIRKMAGGKAKNKEKEKMKDSKDPRYTNGHLFTTITVSGTTMCFVCNKSITAKEALICPTCNVTIHNRCKDALPSCTKVKQKQQKAAFLKNNSALQNVSLRNKTTTLRERPNSAIYPSDSLRHSILGSRRGRASLSLSKSVSTTNIAGNLNDESPLGIRRILSQSTDSLNMRNRTLSVESLIDEGAEVVYSQLMSDFATEEKEFEADSWSLAVDNNYLQQHKKDVMKRQDVIYELIQTEVHHVRTLKIMTNIFRKGMLEDLQMDPALVNKMFPCVDELSDIHIRFLIQLLERRKDSLASDSNKNFVINKLGDILINQFSGTNAERMKKAYTEFASQHHKAMKLYKELFSRDKKFQQMIRKMTRSPLLRRHGVQECILLVTQRITKYPVLLDRILQNSKGDEEEYLDLAESLRMVKELITTIDQDVHNLEKSLRLQEIYQRIDSKSVALVDGDHSFSKEELLRRKLIHEGSLLWKTAAGRFKDVIMLLMTDTMILLQEKDQKYYFPTLDRSPVISLQNLIVRDIANQEKGMFLISAKPPEMYEVHAASREERNTWMKHIGQSVKVCPKREEFPLIETEVEAKLRKLREVIQQKDREVSELLEEKVTLFSKVVQLQAGENSQGPGIGAPRCLFRTDSTDTYRGEKLIGDTIKEVEALKDVIIGAGSELPLLRTEQNHPLDTSNSQSRDSTYGDTNAVNGSCDVAKEDDPKDRNGNQLQNKSPREDALQRIVNIYSFLHGLQAMVIQQDTLQDAPLQDVGDKKERLCRTNSRDPILIEPKSADKQTELTLLQRQHTLLQEELRRCKRQCDDRAQEAGLLETRLRESEQIRGRMERELEDGKRQLLLLRERNDAGLEGARNHKVLDPRRRSLPAGDALYQTFTPPPVHVERRVPLVLDGPVPIITLQDDSDLKDDFSDLTELEKLPAMAETEWTEEEGGGPPASPSSAREFQRMQDIPEEAESVQELRDAEQGSSES

Target ORF information:

RefSeq Version	XM_031890301.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890301.1

ORF Insert Sequence:

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef2 ( XM_031890302.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746162.1 Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X3, mRNA.

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CloneID	OXa63576
Clone ID Related Accession (Same CDS sequence)	XM_031890301.1 , XM_031890302.1 , XM_031890300.1
Accession Version	XM_031890302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746162.1
CDS	210..3791
Translation	MNDAEVFVTGGEAGPFMARVPKQRCLSALDPRMPSEEDLHNEELDGFIAVPLRRSCRLRSSMHTIDAFRRHSWEPGKRMQEMEADYEDLSQSLKGLDPEEIEGNMGNRRDPRRTPIISSTDELESWLSTRGEDQEVTQEEHAKKLQAYLFSQQYLYNPLSKSVSMSGIDRPFCYPDDVTLYSQGRHLSNGALSCGDFDALETDEKENREKEESPLGRTLSFIRKMAGGKAKNKEKEKMKDSKDPRYTNGHLFTTITVSGTTMCFVCNKSITAKEALICPTCNVTIHNRCKDALPSCTKVKQKQQKAAFLKNNSALQNVSLRNKTTTLRERPNSAIYPSDSLRHSILGSRRGRASLSLSKSVSTTNIAGNLNDESPLGIRRILSQSTDSLNMRNRTLSVESLIDEGAEVVYSQLMSDFATEEKEFEADSWSLAVDNNYLQQHKKDVMKRQDVIYELIQTEVHHVRTLKIMTNIFRKGMLEDLQMDPALVNKMFPCVDELSDIHIRFLIQLLERRKDSLASDSNKNFVINKLGDILINQFSGTNAERMKKAYTEFASQHHKAMKLYKELFSRDKKFQQMIRKMTRSPLLRRHGVQECILLVTQRITKYPVLLDRILQNSKGDEEEYLDLAESLRMVKELITTIDQDVHNLEKSLRLQEIYQRIDSKSVALVDGDHSFSKEELLRRKLIHEGSLLWKTAAGRFKDVIMLLMTDTMILLQEKDQKYYFPTLDRSPVISLQNLIVRDIANQEKGMFLISAKPPEMYEVHAASREERNTWMKHIGQSVKVCPKREEFPLIETEVEAKLRKLREVIQQKDREVSELLEEKVTLFSKVVQLQAGENSQGPGIGAPRCLFRTDSTDTYRGEKLIGDTIKEVEALKDVIIGAGSELPLLRTEQNHPLDTSNSQSRDSTYGDTNAVNGSCDVAKEDDPKDRNGNQLQNKSPREDALQRIVNIYSFLHGLQAMVIQQDTLQDAPLQDVGDKKERLCRTNSRDPILIEPKSADKQTELTLLQRQHTLLQEELRRCKRQCDDRAQEAGLLETRLRESEQIRGRMERELEDGKRQLLLLRERNDAGLEGARNHKVLDPRRRSLPAGDALYQTFTPPPVHVERRVPLVLDGPVPIITLQDDSDLKDDFSDLTELEKLPAMAETEWTEEEGGGPPASPSSAREFQRMQDIPEEAESVQELRDAEQGSSES

Target ORF information:

RefSeq Version	XM_031890302.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890302.1

ORF Insert Sequence:

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef2 ( XM_031890300.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746160.1 Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63576
Clone ID Related Accession (Same CDS sequence)	XM_031890301.1 , XM_031890302.1 , XM_031890300.1
Accession Version	XM_031890300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746160.1
CDS	338..3919
Translation	MNDAEVFVTGGEAGPFMARVPKQRCLSALDPRMPSEEDLHNEELDGFIAVPLRRSCRLRSSMHTIDAFRRHSWEPGKRMQEMEADYEDLSQSLKGLDPEEIEGNMGNRRDPRRTPIISSTDELESWLSTRGEDQEVTQEEHAKKLQAYLFSQQYLYNPLSKSVSMSGIDRPFCYPDDVTLYSQGRHLSNGALSCGDFDALETDEKENREKEESPLGRTLSFIRKMAGGKAKNKEKEKMKDSKDPRYTNGHLFTTITVSGTTMCFVCNKSITAKEALICPTCNVTIHNRCKDALPSCTKVKQKQQKAAFLKNNSALQNVSLRNKTTTLRERPNSAIYPSDSLRHSILGSRRGRASLSLSKSVSTTNIAGNLNDESPLGIRRILSQSTDSLNMRNRTLSVESLIDEGAEVVYSQLMSDFATEEKEFEADSWSLAVDNNYLQQHKKDVMKRQDVIYELIQTEVHHVRTLKIMTNIFRKGMLEDLQMDPALVNKMFPCVDELSDIHIRFLIQLLERRKDSLASDSNKNFVINKLGDILINQFSGTNAERMKKAYTEFASQHHKAMKLYKELFSRDKKFQQMIRKMTRSPLLRRHGVQECILLVTQRITKYPVLLDRILQNSKGDEEEYLDLAESLRMVKELITTIDQDVHNLEKSLRLQEIYQRIDSKSVALVDGDHSFSKEELLRRKLIHEGSLLWKTAAGRFKDVIMLLMTDTMILLQEKDQKYYFPTLDRSPVISLQNLIVRDIANQEKGMFLISAKPPEMYEVHAASREERNTWMKHIGQSVKVCPKREEFPLIETEVEAKLRKLREVIQQKDREVSELLEEKVTLFSKVVQLQAGENSQGPGIGAPRCLFRTDSTDTYRGEKLIGDTIKEVEALKDVIIGAGSELPLLRTEQNHPLDTSNSQSRDSTYGDTNAVNGSCDVAKEDDPKDRNGNQLQNKSPREDALQRIVNIYSFLHGLQAMVIQQDTLQDAPLQDVGDKKERLCRTNSRDPILIEPKSADKQTELTLLQRQHTLLQEELRRCKRQCDDRAQEAGLLETRLRESEQIRGRMERELEDGKRQLLLLRERNDAGLEGARNHKVLDPRRRSLPAGDALYQTFTPPPVHVERRVPLVLDGPVPIITLQDDSDLKDDFSDLTELEKLPAMAETEWTEEEGGGPPASPSSAREFQRMQDIPEEAESVQELRDAEQGSSES

Target ORF information:

RefSeq Version	XM_031890300.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890300.1

ORF Insert Sequence:

ATGAATGATG CAGAAGTGTT TGTGACTGGT GGGGAAGCAG GACCCTTCAT GGCGAGGGTT 
CCCAAACAGC GCTGCCTCTC TGCCTTGGAC CCACGGATGC CCAGCGAGGA GGACTTGCAC 
AATGAGGAGC TGGATGGGTT CATTGCGGTG CCGCTGCGCA GATCCTGCCG CCTTCGGTCA 
TCCATGCACA CGATAGATGC ATTCCGCCGG CACAGCTGGG AGCCAGGCAA GCGAATGCAG 
GAGATGGAGG CAGACTATGA GGATCTCAGC CAGAGTTTAA AGGGTCTGGA CCCTGAGGAG 
ATAGAAGGGA ATATGGGGAA CAGGAGGGAC CCCCGTCGCA CTCCGATCAT CAGCAGCACG 
GATGAGCTGG AGTCCTGGCT TTCTACTCGA GGGGAGGATC AGGAAGTCAC GCAGGAGGAA 
CACGCCAAGA AGCTGCAGGC CTACCTCTTT TCCCAGCAAT ATCTGTACAA CCCCCTCTCC 
AAATCTGTCT CTATGTCCGG CATCGACCGC CCCTTCTGCT ACCCCGACGA TGTGACCCTC 
TATTCCCAAG GGAGGCACCT GTCCAACGGC GCCCTGAGCT GCGGAGATTT CGATGCTCTG 
GAGACCGACG AGAAGGAGAA CAGGGAAAAG GAAGAGTCGC CTCTGGGGAG GACCCTGAGC 
TTCATCCGAA AAATGGCCGG AGGGAAAGCC AAGAATAAAG AAAAAGAGAA GATGAAGGAT 
TCCAAGGACC CCCGGTACAC CAACGGGCAC CTGTTCACCA CCATCACCGT GTCTGGGACG 
ACCATGTGCT TTGTGTGCAA CAAGAGTATA ACCGCTAAAG AGGCGCTGAT TTGCCCCACC 
TGTAACGTTA CCATACACAA CCGCTGCAAA GATGCCCTGC CCAGCTGCAC CAAGGTCAAA 
CAGAAACAAC AGAAAGCCGC TTTCCTGAAG AACAATTCTG CCCTGCAGAA CGTCTCGCTG 
AGGAACAAAA CCACGACGCT TCGAGAGCGC CCCAATTCGG CCATCTACCC CTCGGACAGC 
CTGCGCCACT CTATCCTGGG CTCCCGCCGG GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT 
GTCTCCACCA CCAACATTGC AGGGAACCTG AACGATGAGA GCCCACTGGG AATCCGCCGG 
ATCCTCTCCC AATCTACCGA CTCGCTCAAT ATGCGGAACC GGACTCTCTC TGTGGAGTCG 
CTCATCGATG AAGGCGCCGA GGTCGTGTAC AGTCAGCTGA TGAGCGACTT CGCCACCGAG 
GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC CTGGCTGTGG ATAACAACTA CCTCCAGCAG 
CACAAGAAAG ATGTCATGAA GCGGCAGGAC GTGATATACG AGCTGATCCA GACGGAGGTT 
CACCACGTGC GCACCCTGAA GATCATGACC AACATATTCC GCAAGGGGAT GCTGGAGGAT 
CTGCAGATGG ACCCGGCGCT GGTCAATAAG ATGTTCCCCT GCGTGGACGA ACTGAGCGAC 
ATTCACATCC GATTCCTCAT TCAGCTCCTG GAGAGGCGCA AAGACTCGCT GGCGTCCGAC 
AGCAACAAGA ACTTTGTCAT CAACAAGCTG GGGGATATCC TCATTAACCA GTTTTCAGGC 
ACCAATGCCG AGCGGATGAA GAAAGCCTAC ACGGAATTCG CCAGCCAGCA CCATAAGGCC 
ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG GATAAGAAGT TCCAGCAGAT GATACGGAAA 
ATGACCCGCT CCCCCCTGCT GCGCAGGCAC GGCGTCCAGG AATGCATCCT GCTAGTCACC 
CAGCGCATCA CCAAGTACCC CGTGCTCCTT GACCGCATTC TGCAGAACTC TAAGGGCGAT 
GAGGAGGAGT ACCTAGACCT GGCCGAATCC TTGCGGATGG TGAAGGAGCT GATTACGACG 
ATTGACCAAG ACGTGCACAA CCTGGAGAAG AGCTTACGCT TGCAGGAGAT CTACCAGCGA 
ATAGACAGCA AGTCTGTGGC ACTAGTAGAT GGGGACCACA GCTTTAGCAA GGAGGAGCTG 
CTGCGCCGGA AACTCATCCA CGAGGGGTCG CTACTGTGGA AAACGGCTGC TGGGCGCTTC 
AAAGATGTGA TTATGCTGCT AATGACAGAC ACCATGATTC TGCTTCAGGA GAAAGACCAG 
AAATATTATT TCCCAACGCT GGATAGATCT CCCGTCATCA GCCTGCAGAA CCTGATAGTG 
CGGGACATTG CCAACCAGGA GAAGGGCATG TTCCTAATCA GCGCCAAGCC CCCCGAGATG 
TACGAGGTAC ACGCCGCCTC CCGCGAGGAG CGCAACACGT GGATGAAGCA CATCGGGCAG 
TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA TTCCCGCTGA TCGAGACAGA AGTGGAGGCG 
AAACTACGGA AGCTGAGAGA GGTGATTCAG CAGAAGGACC GGGAGGTGTC GGAGCTCCTG 
GAGGAGAAGG TGACGCTCTT TTCCAAAGTG GTGCAGCTCC AGGCGGGGGA GAATTCCCAG 
GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC TTCCGAACCG ACTCCACCGA CACCTACAGG 
GGCGAGAAGC TGATCGGCGA CACCATCAAA GAAGTGGAAG CACTGAAAGA CGTGATCATT 
GGGGCTGGCA GCGAACTGCC TCTTCTACGG ACGGAACAGA ACCACCCATT GGACACTTCC 
AACAGCCAAT CCAGAGATTC CACATATGGG GACACAAACG CCGTCAATGG CTCCTGCGAT 
GTTGCCAAGG AGGACGATCC AAAGGACCGG AACGGAAATC AGCTCCAAAA CAAAAGCCCT 
CGTGAGGACG CCCTACAGAG GATTGTCAAT ATCTACAGCT TTCTACATGG GCTGCAGGCG 
ATGGTCATAC AACAGGACAC GCTACAAGAC GCGCCGCTCC AGGACGTCGG CGACAAGAAG 
GAGCGCCTGT GCCGGACCAA CTCCCGCGAT CCGATTCTGA TAGAACCCAA GAGCGCAGAC 
AAACAGACGG AGCTGACTCT GCTGCAGCGC CAACACACTC TGTTGCAGGA GGAGCTGCGC 
CGGTGCAAGC GGCAGTGCGA CGACAGGGCG CAGGAGGCGG GGCTACTGGA GACCCGGCTG 
AGGGAGAGCG AGCAGATCCG GGGGAGGATG GAGCGGGAGT TGGAGGATGG GAAAAGGCAG 
CTGCTCCTCC TGAGAGAGAG AAACGACGCG GGGCTGGAGG GGGCCAGGAA TCACAAAGTG 
TTGGATCCTC GTAGGCGGAG CTTGCCCGCG GGGGACGCCC TGTATCAGAC CTTCACTCCG 
CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG CTGGTTCTGG ACGGCCCGGT CCCCATCATT 
ACTCTGCAGG ACGACTCGGA CCTCAAAGAT GACTTCAGCG ATCTCACCGA GCTGGAGAAA 
CTGCCGGCCA TGGCGGAAAC GGAATGGACG GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT 
CCCTCCAGCG CACGAGAATT CCAGAGAATG CAGGACATTC CAGAAGAGGC AGAGAGCGTT 
CAGGAGCTGA GAGACGCGGA GCAGGGATCC TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef2 ( XM_031890303.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746163.1 Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X4, mRNA.

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CloneID	OXa63577
Clone ID Related Accession (Same CDS sequence)	XM_031890303.1
Accession Version	XM_031890303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGC AGGGTGGTCA CCGTAACTCC GCCGGGGGTC CCCGGCCCGA CGTGGCGCCT 
CGCCGGCTGC AGACCATGAG CTGGAAGGGT AGGCGCCCAA GCGAGAGGCT CCCTGCCGGA 
CAGAATAAAG AAAAAGAGAA GATGAAGGAT TCCAAGGACC CCCGGTACAC CAACGGGCAC 
CTGTTCACCA CCATCACCGT GTCTGGGACG ACCATGTGCT TTGTGTGCAA CAAGAGTATA 
ACCGCTAAAG AGGCGCTGAT TTGCCCCACC TGTAACGTTA CCATACACAA CCGCTGCAAA 
GATGCCCTGC CCAGCTGCAC CAAGGTCAAA CAGAAACAAC AGAAAGCCGC TTTCCTGAAG 
AACAATTCTG CCCTGCAGAA CGTCTCGCTG AGGAACAAAA CCACGACGCT TCGAGAGCGC 
CCCAATTCGG CCATCTACCC CTCGGACAGC CTGCGCCACT CTATCCTGGG CTCCCGCCGG 
GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT GTCTCCACCA CCAACATTGC AGGGAACCTG 
AACGATGAGA GCCCACTGGG AATCCGCCGG ATCCTCTCCC AATCTACCGA CTCGCTCAAT 
ATGCGGAACC GGACTCTCTC TGTGGAGTCG CTCATCGATG AAGGCGCCGA GGTCGTGTAC 
AGTCAGCTGA TGAGCGACTT CGCCACCGAG GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC 
CTGGCTGTGG ATAACAACTA CCTCCAGCAG CACAAGAAAG ATGTCATGAA GCGGCAGGAC 
GTGATATACG AGCTGATCCA GACGGAGGTT CACCACGTGC GCACCCTGAA GATCATGACC 
AACATATTCC GCAAGGGGAT GCTGGAGGAT CTGCAGATGG ACCCGGCGCT GGTCAATAAG 
ATGTTCCCCT GCGTGGACGA ACTGAGCGAC ATTCACATCC GATTCCTCAT TCAGCTCCTG 
GAGAGGCGCA AAGACTCGCT GGCGTCCGAC AGCAACAAGA ACTTTGTCAT CAACAAGCTG 
GGGGATATCC TCATTAACCA GTTTTCAGGC ACCAATGCCG AGCGGATGAA GAAAGCCTAC 
ACGGAATTCG CCAGCCAGCA CCATAAGGCC ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG 
GATAAGAAGT TCCAGCAGAT GATACGGAAA ATGACCCGCT CCCCCCTGCT GCGCAGGCAC 
GGCGTCCAGG AATGCATCCT GCTAGTCACC CAGCGCATCA CCAAGTACCC CGTGCTCCTT 
GACCGCATTC TGCAGAACTC TAAGGGCGAT GAGGAGGAGT ACCTAGACCT GGCCGAATCC 
TTGCGGATGG TGAAGGAGCT GATTACGACG ATTGACCAAG ACGTGCACAA CCTGGAGAAG 
AGCTTACGCT TGCAGGAGAT CTACCAGCGA ATAGACAGCA AGTCTGTGGC ACTAGTAGAT 
GGGGACCACA GCTTTAGCAA GGAGGAGCTG CTGCGCCGGA AACTCATCCA CGAGGGGTCG 
CTACTGTGGA AAACGGCTGC TGGGCGCTTC AAAGATGTGA TTATGCTGCT AATGACAGAC 
ACCATGATTC TGCTTCAGGA GAAAGACCAG AAATATTATT TCCCAACGCT GGATAGATCT 
CCCGTCATCA GCCTGCAGAA CCTGATAGTG CGGGACATTG CCAACCAGGA GAAGGGCATG 
TTCCTAATCA GCGCCAAGCC CCCCGAGATG TACGAGGTAC ACGCCGCCTC CCGCGAGGAG 
CGCAACACGT GGATGAAGCA CATCGGGCAG TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA 
TTCCCGCTGA TCGAGACAGA AGTGGAGGCG AAACTACGGA AGCTGAGAGA GGTGATTCAG 
CAGAAGGACC GGGAGGTGTC GGAGCTCCTG GAGGAGAAGG TGACGCTCTT TTCCAAAGTG 
GTGCAGCTCC AGGCGGGGGA GAATTCCCAG GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC 
TTCCGAACCG ACTCCACCGA CACCTACAGG GGCGAGAAGC TGATCGGCGA CACCATCAAA 
GAAGTGGAAG CACTGAAAGA CGTGATCATT GGGGCTGGCA GCGAACTGCC TCTTCTACGG 
ACGGAACAGA ACCACCCATT GGACACTTCC AACAGCCAAT CCAGAGATTC CACATATGGG 
GACACAAACG CCGTCAATGG CTCCTGCGAT GTTGCCAAGG AGGACGATCC AAAGGACCGG 
AACGGAAATC AGCTCCAAAA CAAAAGCCCT CGTGAGGACG CCCTACAGAG GATTGTCAAT 
ATCTACAGCT TTCTACATGG GCTGCAGGCG ATGGTCATAC AACAGGACAC GCTACAAGAC 
GCGCCGCTCC AGGACGTCGG CGACAAGAAG GAGCGCCTGT GCCGGACCAA CTCCCGCGAT 
CCGATTCTGA TAGAACCCAA GAGCGCAGAC AAACAGACGG AGCTGACTCT GCTGCAGCGC 
CAACACACTC TGTTGCAGGA GGAGCTGCGC CGGTGCAAGC GGCAGTGCGA CGACAGGGCG 
CAGGAGGCGG GGCTACTGGA GACCCGGCTG AGGGAGAGCG AGCAGATCCG GGGGAGGATG 
GAGCGGGAGT TGGAGGATGG GAAAAGGCAG CTGCTCCTCC TGAGAGAGAG AAACGACGCG 
GGGCTGGAGG GGGCCAGGAA TCACAAAGTG TTGGATCCTC GTAGGCGGAG CTTGCCCGCG 
GGGGACGCCC TGTATCAGAC CTTCACTCCG CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG 
CTGGTTCTGG ACGGCCCGGT CCCCATCATT ACTCTGCAGG ACGACTCGGA CCTCAAAGAT 
GACTTCAGCG ATCTCACCGA GCTGGAGAAA CTGCCGGCCA TGGCGGAAAC GGAATGGACG 
GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT CCCTCCAGCG CACGAGAATT CCAGAGAATG 
CAGGACATTC CAGAAGAGGC AGAGAGCGTT CAGGAGCTGA GAGACGCGGA GCAGGGATCC 
TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746163.1
CDS	12..3023
Translation	MQQQGGHRNSAGGPRPDVAPRRLQTMSWKGRRPSERLPAGQNKEKEKMKDSKDPRYTNGHLFTTITVSGTTMCFVCNKSITAKEALICPTCNVTIHNRCKDALPSCTKVKQKQQKAAFLKNNSALQNVSLRNKTTTLRERPNSAIYPSDSLRHSILGSRRGRASLSLSKSVSTTNIAGNLNDESPLGIRRILSQSTDSLNMRNRTLSVESLIDEGAEVVYSQLMSDFATEEKEFEADSWSLAVDNNYLQQHKKDVMKRQDVIYELIQTEVHHVRTLKIMTNIFRKGMLEDLQMDPALVNKMFPCVDELSDIHIRFLIQLLERRKDSLASDSNKNFVINKLGDILINQFSGTNAERMKKAYTEFASQHHKAMKLYKELFSRDKKFQQMIRKMTRSPLLRRHGVQECILLVTQRITKYPVLLDRILQNSKGDEEEYLDLAESLRMVKELITTIDQDVHNLEKSLRLQEIYQRIDSKSVALVDGDHSFSKEELLRRKLIHEGSLLWKTAAGRFKDVIMLLMTDTMILLQEKDQKYYFPTLDRSPVISLQNLIVRDIANQEKGMFLISAKPPEMYEVHAASREERNTWMKHIGQSVKVCPKREEFPLIETEVEAKLRKLREVIQQKDREVSELLEEKVTLFSKVVQLQAGENSQGPGIGAPRCLFRTDSTDTYRGEKLIGDTIKEVEALKDVIIGAGSELPLLRTEQNHPLDTSNSQSRDSTYGDTNAVNGSCDVAKEDDPKDRNGNQLQNKSPREDALQRIVNIYSFLHGLQAMVIQQDTLQDAPLQDVGDKKERLCRTNSRDPILIEPKSADKQTELTLLQRQHTLLQEELRRCKRQCDDRAQEAGLLETRLRESEQIRGRMERELEDGKRQLLLLRERNDAGLEGARNHKVLDPRRRSLPAGDALYQTFTPPPVHVERRVPLVLDGPVPIITLQDDSDLKDDFSDLTELEKLPAMAETEWTEEEGGGPPASPSSAREFQRMQDIPEEAESVQELRDAEQGSSES

Target ORF information:

RefSeq Version	XM_031890303.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho/Rac guanine nucleotide exchange factor 2 (arhgef2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890303.1

ORF Insert Sequence:

ATGCAGCAGC AGGGTGGTCA CCGTAACTCC GCCGGGGGTC CCCGGCCCGA CGTGGCGCCT 
CGCCGGCTGC AGACCATGAG CTGGAAGGGT AGGCGCCCAA GCGAGAGGCT CCCTGCCGGA 
CAGAATAAAG AAAAAGAGAA GATGAAGGAT TCCAAGGACC CCCGGTACAC CAACGGGCAC 
CTGTTCACCA CCATCACCGT GTCTGGGACG ACCATGTGCT TTGTGTGCAA CAAGAGTATA 
ACCGCTAAAG AGGCGCTGAT TTGCCCCACC TGTAACGTTA CCATACACAA CCGCTGCAAA 
GATGCCCTGC CCAGCTGCAC CAAGGTCAAA CAGAAACAAC AGAAAGCCGC TTTCCTGAAG 
AACAATTCTG CCCTGCAGAA CGTCTCGCTG AGGAACAAAA CCACGACGCT TCGAGAGCGC 
CCCAATTCGG CCATCTACCC CTCGGACAGC CTGCGCCACT CTATCCTGGG CTCCCGCCGG 
GGCCGGGCGT CTCTTTCTCT GTCTAAAAGT GTCTCCACCA CCAACATTGC AGGGAACCTG 
AACGATGAGA GCCCACTGGG AATCCGCCGG ATCCTCTCCC AATCTACCGA CTCGCTCAAT 
ATGCGGAACC GGACTCTCTC TGTGGAGTCG CTCATCGATG AAGGCGCCGA GGTCGTGTAC 
AGTCAGCTGA TGAGCGACTT CGCCACCGAG GAGAAGGAAT TCGAGGCGGA TTCGTGGAGC 
CTGGCTGTGG ATAACAACTA CCTCCAGCAG CACAAGAAAG ATGTCATGAA GCGGCAGGAC 
GTGATATACG AGCTGATCCA GACGGAGGTT CACCACGTGC GCACCCTGAA GATCATGACC 
AACATATTCC GCAAGGGGAT GCTGGAGGAT CTGCAGATGG ACCCGGCGCT GGTCAATAAG 
ATGTTCCCCT GCGTGGACGA ACTGAGCGAC ATTCACATCC GATTCCTCAT TCAGCTCCTG 
GAGAGGCGCA AAGACTCGCT GGCGTCCGAC AGCAACAAGA ACTTTGTCAT CAACAAGCTG 
GGGGATATCC TCATTAACCA GTTTTCAGGC ACCAATGCCG AGCGGATGAA GAAAGCCTAC 
ACGGAATTCG CCAGCCAGCA CCATAAGGCC ATGAAGCTGT ACAAGGAGCT CTTCTCCCGG 
GATAAGAAGT TCCAGCAGAT GATACGGAAA ATGACCCGCT CCCCCCTGCT GCGCAGGCAC 
GGCGTCCAGG AATGCATCCT GCTAGTCACC CAGCGCATCA CCAAGTACCC CGTGCTCCTT 
GACCGCATTC TGCAGAACTC TAAGGGCGAT GAGGAGGAGT ACCTAGACCT GGCCGAATCC 
TTGCGGATGG TGAAGGAGCT GATTACGACG ATTGACCAAG ACGTGCACAA CCTGGAGAAG 
AGCTTACGCT TGCAGGAGAT CTACCAGCGA ATAGACAGCA AGTCTGTGGC ACTAGTAGAT 
GGGGACCACA GCTTTAGCAA GGAGGAGCTG CTGCGCCGGA AACTCATCCA CGAGGGGTCG 
CTACTGTGGA AAACGGCTGC TGGGCGCTTC AAAGATGTGA TTATGCTGCT AATGACAGAC 
ACCATGATTC TGCTTCAGGA GAAAGACCAG AAATATTATT TCCCAACGCT GGATAGATCT 
CCCGTCATCA GCCTGCAGAA CCTGATAGTG CGGGACATTG CCAACCAGGA GAAGGGCATG 
TTCCTAATCA GCGCCAAGCC CCCCGAGATG TACGAGGTAC ACGCCGCCTC CCGCGAGGAG 
CGCAACACGT GGATGAAGCA CATCGGGCAG TCGGTCAAAG TGTGCCCAAA GCGAGAGGAA 
TTCCCGCTGA TCGAGACAGA AGTGGAGGCG AAACTACGGA AGCTGAGAGA GGTGATTCAG 
CAGAAGGACC GGGAGGTGTC GGAGCTCCTG GAGGAGAAGG TGACGCTCTT TTCCAAAGTG 
GTGCAGCTCC AGGCGGGGGA GAATTCCCAG GGGCCCGGCA TCGGCGCCCC CCGCTGCCTC 
TTCCGAACCG ACTCCACCGA CACCTACAGG GGCGAGAAGC TGATCGGCGA CACCATCAAA 
GAAGTGGAAG CACTGAAAGA CGTGATCATT GGGGCTGGCA GCGAACTGCC TCTTCTACGG 
ACGGAACAGA ACCACCCATT GGACACTTCC AACAGCCAAT CCAGAGATTC CACATATGGG 
GACACAAACG CCGTCAATGG CTCCTGCGAT GTTGCCAAGG AGGACGATCC AAAGGACCGG 
AACGGAAATC AGCTCCAAAA CAAAAGCCCT CGTGAGGACG CCCTACAGAG GATTGTCAAT 
ATCTACAGCT TTCTACATGG GCTGCAGGCG ATGGTCATAC AACAGGACAC GCTACAAGAC 
GCGCCGCTCC AGGACGTCGG CGACAAGAAG GAGCGCCTGT GCCGGACCAA CTCCCGCGAT 
CCGATTCTGA TAGAACCCAA GAGCGCAGAC AAACAGACGG AGCTGACTCT GCTGCAGCGC 
CAACACACTC TGTTGCAGGA GGAGCTGCGC CGGTGCAAGC GGCAGTGCGA CGACAGGGCG 
CAGGAGGCGG GGCTACTGGA GACCCGGCTG AGGGAGAGCG AGCAGATCCG GGGGAGGATG 
GAGCGGGAGT TGGAGGATGG GAAAAGGCAG CTGCTCCTCC TGAGAGAGAG AAACGACGCG 
GGGCTGGAGG GGGCCAGGAA TCACAAAGTG TTGGATCCTC GTAGGCGGAG CTTGCCCGCG 
GGGGACGCCC TGTATCAGAC CTTCACTCCG CCCCCGGTTC ACGTGGAGAG GAGGGTCCCG 
CTGGTTCTGG ACGGCCCGGT CCCCATCATT ACTCTGCAGG ACGACTCGGA CCTCAAAGAT 
GACTTCAGCG ATCTCACCGA GCTGGAGAAA CTGCCGGCCA TGGCGGAAAC GGAATGGACG 
GAGGAGGAGG GCGGGGGGCC CCCAGCTTCT CCCTCCAGCG CACGAGAATT CCAGAGAATG 
CAGGACATTC CAGAAGAGGC AGAGAGCGTT CAGGAGCTGA GAGACGCGGA GCAGGGATCC 
TCCGAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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arhgef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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