smg7 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012960767.2	XP_012816221.1	protein SMG7 isoform X1
XM_012960768.2	XP_012816222.1	protein SMG7 isoform X2
XM_012960769.2	XP_012816223.1	protein SMG7 isoform X3
XM_012960769.1	XP_012816223.1	protein SMG7 isoform X3
XM_012960770.2	XP_012816224.1	protein SMG7 isoform X4
NM_203927.1	NP_989258.1	protein SMG7
XM_012960767.1	XP_012816221.1	protein SMG7 isoform X1
XM_012960768.1	XP_012816222.1	protein SMG7 isoform X2
XM_012960770.1	XP_012816224.1	protein SMG7 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa26127	XM_012960767.2 Latest version!	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa26128	XM_012960768.2 Latest version!	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa26129	XM_012960769.2 Latest version!	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa26130	XM_012960770.2 Latest version!	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa22350	NM_203927.1 Latest version!	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa26129	XM_012960769.1	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26127	XM_012960767.1	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa26128	XM_012960768.1	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa26130	XM_012960770.1	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

smg7 ( XM_012960767.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816221.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26127
Clone ID Related Accession (Same CDS sequence)	XM_012960767.2 , XM_012960767.1
Accession Version	XM_012960767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTAA GTCTCTCCTG GAAAAGCCAT CTGAACTGAT ATCCCAGCCC 
CCTTCTTTCC TCTCACTCTC TGGATTTTCA CTAAGCCAGG AACGGTTCCC GAATAACAGC 
ATGTTTAATG AGGTGTATGG AAAATCCACC TGCACGAGCA TAAAGCCCGA GGCATGCCAA 
GTCATGGGAC AACAGGAGAC CTCCCTGTAC TCTCTTTTTG AGGGTAAACC ATGGTCTCCA 
TCACTTCCAG CAAGCTCAGA TCATTCTACG CCAGCCAGCC AGTCACCTCA TTCTTCAAAT 
CCAAGCAGCC TACCCAGCTC TCCTCCCACC CACAATCATA CCTCGCAGCC CTTCTCTAAC 
TTTGGTCCTA TCGGCACTCC AGATAACCGA GATCGTCGGG GAACTGACCG CTGGAAGGCT 
GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT GGGCTGGACT ACTTATCTGT GACTCCATCT 
TCTGAGAATA GCTGGCGCCA GGCTAATAAT TCAAGTAGTG CTAATGCTTG GGCTGCTCAA 
GGTAATGCCC GAGAGGACTC TTCAGCTGCT CTTATGGAAA GTTTAAAGTC CATTTGGTCA 
ACCACAATGA TGCATCCTGG ACCTTCTGCC TTGGAACAGC TTCTGATGCA GCAGAAGGAG 
AAGCAGCAGC GAGGGCAGGG ATCACTGAAC CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816221.1
CDS	254..3835
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEEAKRSPLLPPDLLKSLAALEEEEELAFSPPPDLYPALFGPLASLPGRSLFKSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960767.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960767.2

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTAA GTCTCTCCTG GAAAAGCCAT CTGAACTGAT ATCCCAGCCC 
CCTTCTTTCC TCTCACTCTC TGGATTTTCA CTAAGCCAGG AACGGTTCCC GAATAACAGC 
ATGTTTAATG AGGTGTATGG AAAATCCACC TGCACGAGCA TAAAGCCCGA GGCATGCCAA 
GTCATGGGAC AACAGGAGAC CTCCCTGTAC TCTCTTTTTG AGGGTAAACC ATGGTCTCCA 
TCACTTCCAG CAAGCTCAGA TCATTCTACG CCAGCCAGCC AGTCACCTCA TTCTTCAAAT 
CCAAGCAGCC TACCCAGCTC TCCTCCCACC CACAATCATA CCTCGCAGCC CTTCTCTAAC 
TTTGGTCCTA TCGGCACTCC AGATAACCGA GATCGTCGGG GAACTGACCG CTGGAAGGCT 
GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT GGGCTGGACT ACTTATCTGT GACTCCATCT 
TCTGAGAATA GCTGGCGCCA GGCTAATAAT TCAAGTAGTG CTAATGCTTG GGCTGCTCAA 
GGTAATGCCC GAGAGGACTC TTCAGCTGCT CTTATGGAAA GTTTAAAGTC CATTTGGTCA 
ACCACAATGA TGCATCCTGG ACCTTCTGCC TTGGAACAGC TTCTGATGCA GCAGAAGGAG 
AAGCAGCAGC GAGGGCAGGG ATCACTGAAC CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960768.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816222.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26128
Clone ID Related Accession (Same CDS sequence)	XM_012960768.2 , XM_012960768.1
Accession Version	XM_012960768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTTC TCTCCTGGAA AAGCCATCTG AACTGATATC CCAGCCCCCT 
TCTTTCCTCT CACTCTCTGG ATTTTCACTA AGCCAGGAAC GGTTCCCGAA TAACAGCATG 
TTTAATGAGG TGTATGGAAA ATCCACCTGC ACGAGCATAA AGCCCGAGGC ATGCCAAGTC 
ATGGGACAAC AGGAGACCTC CCTGTACTCT CTTTTTGAGG GTAAACCATG GTCTCCATCA 
CTTCCAGCAA GCTCAGATCA TTCTACGCCA GCCAGCCAGT CACCTCATTC TTCAAATCCA 
AGCAGCCTAC CCAGCTCTCC TCCCACCCAC AATCATACCT CGCAGCCCTT CTCTAACTTT 
GGTCCTATCG GCACTCCAGA TAACCGAGAT CGTCGGGGAA CTGACCGCTG GAAGGCTGAA 
AAGCCAGTTT CAGCAATGGG TAGCTTTGGG CTGGACTACT TATCTGTGAC TCCATCTTCT 
GAGAATAGCT GGCGCCAGGC TAATAATTCA AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT 
AATGCCCGAG AGGACTCTTC AGCTGCTCTT ATGGAAAGTT TAAAGTCCAT TTGGTCAACC 
ACAATGATGC ATCCTGGACC TTCTGCCTTG GAACAGCTTC TGATGCAGCA GAAGGAGAAG 
CAGCAGCGAG GGCAGGGATC ACTGAACCCT CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816222.1
CDS	254..3832
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEEAKRSPLLPPDLLKSLAALEEEEELAFSPPPDLYPALFGPLASLPGRSLFSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960768.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960768.2

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTTC TCTCCTGGAA AAGCCATCTG AACTGATATC CCAGCCCCCT 
TCTTTCCTCT CACTCTCTGG ATTTTCACTA AGCCAGGAAC GGTTCCCGAA TAACAGCATG 
TTTAATGAGG TGTATGGAAA ATCCACCTGC ACGAGCATAA AGCCCGAGGC ATGCCAAGTC 
ATGGGACAAC AGGAGACCTC CCTGTACTCT CTTTTTGAGG GTAAACCATG GTCTCCATCA 
CTTCCAGCAA GCTCAGATCA TTCTACGCCA GCCAGCCAGT CACCTCATTC TTCAAATCCA 
AGCAGCCTAC CCAGCTCTCC TCCCACCCAC AATCATACCT CGCAGCCCTT CTCTAACTTT 
GGTCCTATCG GCACTCCAGA TAACCGAGAT CGTCGGGGAA CTGACCGCTG GAAGGCTGAA 
AAGCCAGTTT CAGCAATGGG TAGCTTTGGG CTGGACTACT TATCTGTGAC TCCATCTTCT 
GAGAATAGCT GGCGCCAGGC TAATAATTCA AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT 
AATGCCCGAG AGGACTCTTC AGCTGCTCTT ATGGAAAGTT TAAAGTCCAT TTGGTCAACC 
ACAATGATGC ATCCTGGACC TTCTGCCTTG GAACAGCTTC TGATGCAGCA GAAGGAGAAG 
CAGCAGCGAG GGCAGGGATC ACTGAACCCT CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960769.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816223.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.

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CloneID	OXa26129
Clone ID Related Accession (Same CDS sequence)	XM_012960769.2 , XM_012960769.1
Accession Version	XM_012960769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGAA GTCTCTCCTG 
GAAAAGCCAT CTGAACTGAT ATCCCAGCCC CCTTCTTTCC TCTCACTCTC TGGATTTTCA 
CTAAGCCAGG AACGGTTCCC GAATAACAGC ATGTTTAATG AGGTGTATGG AAAATCCACC 
TGCACGAGCA TAAAGCCCGA GGCATGCCAA GTCATGGGAC AACAGGAGAC CTCCCTGTAC 
TCTCTTTTTG AGGGTAAACC ATGGTCTCCA TCACTTCCAG CAAGCTCAGA TCATTCTACG 
CCAGCCAGCC AGTCACCTCA TTCTTCAAAT CCAAGCAGCC TACCCAGCTC TCCTCCCACC 
CACAATCATA CCTCGCAGCC CTTCTCTAAC TTTGGTCCTA TCGGCACTCC AGATAACCGA 
GATCGTCGGG GAACTGACCG CTGGAAGGCT GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT 
GGGCTGGACT ACTTATCTGT GACTCCATCT TCTGAGAATA GCTGGCGCCA GGCTAATAAT 
TCAAGTAGTG CTAATGCTTG GGCTGCTCAA GGTAATGCCC GAGAGGACTC TTCAGCTGCT 
CTTATGGAAA GTTTAAAGTC CATTTGGTCA ACCACAATGA TGCATCCTGG ACCTTCTGCC 
TTGGAACAGC TTCTGATGCA GCAGAAGGAG AAGCAGCAGC GAGGGCAGGG ATCACTGAAC 
CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816223.1
CDS	254..3685
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEKSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960769.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960769.2

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGAA GTCTCTCCTG 
GAAAAGCCAT CTGAACTGAT ATCCCAGCCC CCTTCTTTCC TCTCACTCTC TGGATTTTCA 
CTAAGCCAGG AACGGTTCCC GAATAACAGC ATGTTTAATG AGGTGTATGG AAAATCCACC 
TGCACGAGCA TAAAGCCCGA GGCATGCCAA GTCATGGGAC AACAGGAGAC CTCCCTGTAC 
TCTCTTTTTG AGGGTAAACC ATGGTCTCCA TCACTTCCAG CAAGCTCAGA TCATTCTACG 
CCAGCCAGCC AGTCACCTCA TTCTTCAAAT CCAAGCAGCC TACCCAGCTC TCCTCCCACC 
CACAATCATA CCTCGCAGCC CTTCTCTAAC TTTGGTCCTA TCGGCACTCC AGATAACCGA 
GATCGTCGGG GAACTGACCG CTGGAAGGCT GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT 
GGGCTGGACT ACTTATCTGT GACTCCATCT TCTGAGAATA GCTGGCGCCA GGCTAATAAT 
TCAAGTAGTG CTAATGCTTG GGCTGCTCAA GGTAATGCCC GAGAGGACTC TTCAGCTGCT 
CTTATGGAAA GTTTAAAGTC CATTTGGTCA ACCACAATGA TGCATCCTGG ACCTTCTGCC 
TTGGAACAGC TTCTGATGCA GCAGAAGGAG AAGCAGCAGC GAGGGCAGGG ATCACTGAAC 
CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960770.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816224.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa26130
Clone ID Related Accession (Same CDS sequence)	XM_012960770.2 , XM_012960770.1
Accession Version	XM_012960770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA ACGGTTCCCG 
AATAACAGCA TGTTTAATGA GGTGTATGGA AAATCCACCT GCACGAGCAT AAAGCCCGAG 
GCATGCCAAG TCATGGGACA ACAGGAGACC TCCCTGTACT CTCTTTTTGA GGGTAAACCA 
TGGTCTCCAT CACTTCCAGC AAGCTCAGAT CATTCTACGC CAGCCAGCCA GTCACCTCAT 
TCTTCAAATC CAAGCAGCCT ACCCAGCTCT CCTCCCACCC ACAATCATAC CTCGCAGCCC 
TTCTCTAACT TTGGTCCTAT CGGCACTCCA GATAACCGAG ATCGTCGGGG AACTGACCGC 
TGGAAGGCTG AAAAGCCAGT TTCAGCAATG GGTAGCTTTG GGCTGGACTA CTTATCTGTG 
ACTCCATCTT CTGAGAATAG CTGGCGCCAG GCTAATAATT CAAGTAGTGC TAATGCTTGG 
GCTGCTCAAG GTAATGCCCG AGAGGACTCT TCAGCTGCTC TTATGGAAAG TTTAAAGTCC 
ATTTGGTCAA CCACAATGAT GCATCCTGGA CCTTCTGCCT TGGAACAGCT TCTGATGCAG 
CAGAAGGAGA AGCAGCAGCG AGGGCAGGGA TCACTGAACC CTCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816224.1
CDS	254..3604
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960770.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960770.2

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA ACGGTTCCCG 
AATAACAGCA TGTTTAATGA GGTGTATGGA AAATCCACCT GCACGAGCAT AAAGCCCGAG 
GCATGCCAAG TCATGGGACA ACAGGAGACC TCCCTGTACT CTCTTTTTGA GGGTAAACCA 
TGGTCTCCAT CACTTCCAGC AAGCTCAGAT CATTCTACGC CAGCCAGCCA GTCACCTCAT 
TCTTCAAATC CAAGCAGCCT ACCCAGCTCT CCTCCCACCC ACAATCATAC CTCGCAGCCC 
TTCTCTAACT TTGGTCCTAT CGGCACTCCA GATAACCGAG ATCGTCGGGG AACTGACCGC 
TGGAAGGCTG AAAAGCCAGT TTCAGCAATG GGTAGCTTTG GGCTGGACTA CTTATCTGTG 
ACTCCATCTT CTGAGAATAG CTGGCGCCAG GCTAATAATT CAAGTAGTGC TAATGCTTGG 
GCTGCTCAAG GTAATGCCCG AGAGGACTCT TCAGCTGCTC TTATGGAAAG TTTAAAGTCC 
ATTTGGTCAA CCACAATGAT GCATCCTGGA CCTTCTGCCT TGGAACAGCT TCTGATGCAG 
CAGAAGGAGA AGCAGCAGCG AGGGCAGGGA TCACTGAACC CTCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( NM_203927.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_989258.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), mRNA.

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CloneID	OXa22350
Clone ID Related Accession (Same CDS sequence)	NM_203927.1
Accession Version	NM_203927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063908.1. ##Evidence-Data-START## Transcript exon combination :: BC063908.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGTC TCTCCTGGAA 
AAGCCATCTG AACTGATATC CCAGCCCCCT TCTTTCCTCT CACTCTCTGG ATTTTCACTA 
AGCCAGGAAC GGTTCCCGAA TAACAGCATG TTTAATGAGG TGTATGGAAA ATCCACCTGC 
ACGAGCATAA AGCCCGAGGC ATGCCAAGTC ATGGGACAAC AGGAGACCTC CCTGTACTCT 
CTTTTTGAGG GTAAACCATG GTCTCCATCA CTTCCAGCAA GCTCAGATCA TTCTACGCCA 
GCCAGCCAGT CACCTCATTC TTCAAATCCA AGCAGCCTAC CCAGCTCTCC TCCCACCCAC 
AATCATACCT CGCAGCCCTT CTCTAACTTT GGTCCTATCG GCACTCCAGA TAACCGAGAT 
CGTCGGGGAA CTGACCGCTG GAAGGCTGAA AAGCCAGTTT CAGCAATGGG TAGCTTTGGG 
CTGGACTACT TATCTGTGAC TCCATCTTCT GAGAATAGCT GGCGCCAGGC TAATAATTCA 
AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT AATGCCCGAG AGGACTCTTC AGCTGCTCTT 
ATGGAAAGTT TAAAGTCCAT TTGGTCAACC ACAATGATGC ATCCTGGACC TTCTGCCTTG 
GAACAGCTTC TGATGCAGCA GAAGGAGAAG CAGCAGCGAG GGCAGGGATC ACTGAACCCT 
CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989258.1
CDS	166..3594
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFESLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	NM_203927.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203927.1

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGTC TCTCCTGGAA 
AAGCCATCTG AACTGATATC CCAGCCCCCT TCTTTCCTCT CACTCTCTGG ATTTTCACTA 
AGCCAGGAAC GGTTCCCGAA TAACAGCATG TTTAATGAGG TGTATGGAAA ATCCACCTGC 
ACGAGCATAA AGCCCGAGGC ATGCCAAGTC ATGGGACAAC AGGAGACCTC CCTGTACTCT 
CTTTTTGAGG GTAAACCATG GTCTCCATCA CTTCCAGCAA GCTCAGATCA TTCTACGCCA 
GCCAGCCAGT CACCTCATTC TTCAAATCCA AGCAGCCTAC CCAGCTCTCC TCCCACCCAC 
AATCATACCT CGCAGCCCTT CTCTAACTTT GGTCCTATCG GCACTCCAGA TAACCGAGAT 
CGTCGGGGAA CTGACCGCTG GAAGGCTGAA AAGCCAGTTT CAGCAATGGG TAGCTTTGGG 
CTGGACTACT TATCTGTGAC TCCATCTTCT GAGAATAGCT GGCGCCAGGC TAATAATTCA 
AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT AATGCCCGAG AGGACTCTTC AGCTGCTCTT 
ATGGAAAGTT TAAAGTCCAT TTGGTCAACC ACAATGATGC ATCCTGGACC TTCTGCCTTG 
GAACAGCTTC TGATGCAGCA GAAGGAGAAG CAGCAGCGAG GGCAGGGATC ACTGAACCCT 
CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960767.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816221.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

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CloneID	OXa26127
Clone ID Related Accession (Same CDS sequence)	XM_012960767.2 , XM_012960767.1
Accession Version	XM_012960767.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTAA GTCTCTCCTG GAAAAGCCAT CTGAACTGAT ATCCCAGCCC 
CCTTCTTTCC TCTCACTCTC TGGATTTTCA CTAAGCCAGG AACGGTTCCC GAATAACAGC 
ATGTTTAATG AGGTGTATGG AAAATCCACC TGCACGAGCA TAAAGCCCGA GGCATGCCAA 
GTCATGGGAC AACAGGAGAC CTCCCTGTAC TCTCTTTTTG AGGGTAAACC ATGGTCTCCA 
TCACTTCCAG CAAGCTCAGA TCATTCTACG CCAGCCAGCC AGTCACCTCA TTCTTCAAAT 
CCAAGCAGCC TACCCAGCTC TCCTCCCACC CACAATCATA CCTCGCAGCC CTTCTCTAAC 
TTTGGTCCTA TCGGCACTCC AGATAACCGA GATCGTCGGG GAACTGACCG CTGGAAGGCT 
GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT GGGCTGGACT ACTTATCTGT GACTCCATCT 
TCTGAGAATA GCTGGCGCCA GGCTAATAAT TCAAGTAGTG CTAATGCTTG GGCTGCTCAA 
GGTAATGCCC GAGAGGACTC TTCAGCTGCT CTTATGGAAA GTTTAAAGTC CATTTGGTCA 
ACCACAATGA TGCATCCTGG ACCTTCTGCC TTGGAACAGC TTCTGATGCA GCAGAAGGAG 
AAGCAGCAGC GAGGGCAGGG ATCACTGAAC CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816221.1
CDS	254..3835
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEEAKRSPLLPPDLLKSLAALEEEEELAFSPPPDLYPALFGPLASLPGRSLFKSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960767.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960767.1

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTAA GTCTCTCCTG GAAAAGCCAT CTGAACTGAT ATCCCAGCCC 
CCTTCTTTCC TCTCACTCTC TGGATTTTCA CTAAGCCAGG AACGGTTCCC GAATAACAGC 
ATGTTTAATG AGGTGTATGG AAAATCCACC TGCACGAGCA TAAAGCCCGA GGCATGCCAA 
GTCATGGGAC AACAGGAGAC CTCCCTGTAC TCTCTTTTTG AGGGTAAACC ATGGTCTCCA 
TCACTTCCAG CAAGCTCAGA TCATTCTACG CCAGCCAGCC AGTCACCTCA TTCTTCAAAT 
CCAAGCAGCC TACCCAGCTC TCCTCCCACC CACAATCATA CCTCGCAGCC CTTCTCTAAC 
TTTGGTCCTA TCGGCACTCC AGATAACCGA GATCGTCGGG GAACTGACCG CTGGAAGGCT 
GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT GGGCTGGACT ACTTATCTGT GACTCCATCT 
TCTGAGAATA GCTGGCGCCA GGCTAATAAT TCAAGTAGTG CTAATGCTTG GGCTGCTCAA 
GGTAATGCCC GAGAGGACTC TTCAGCTGCT CTTATGGAAA GTTTAAAGTC CATTTGGTCA 
ACCACAATGA TGCATCCTGG ACCTTCTGCC TTGGAACAGC TTCTGATGCA GCAGAAGGAG 
AAGCAGCAGC GAGGGCAGGG ATCACTGAAC CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960768.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816222.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26128
Clone ID Related Accession (Same CDS sequence)	XM_012960768.2 , XM_012960768.1
Accession Version	XM_012960768.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTTC TCTCCTGGAA AAGCCATCTG AACTGATATC CCAGCCCCCT 
TCTTTCCTCT CACTCTCTGG ATTTTCACTA AGCCAGGAAC GGTTCCCGAA TAACAGCATG 
TTTAATGAGG TGTATGGAAA ATCCACCTGC ACGAGCATAA AGCCCGAGGC ATGCCAAGTC 
ATGGGACAAC AGGAGACCTC CCTGTACTCT CTTTTTGAGG GTAAACCATG GTCTCCATCA 
CTTCCAGCAA GCTCAGATCA TTCTACGCCA GCCAGCCAGT CACCTCATTC TTCAAATCCA 
AGCAGCCTAC CCAGCTCTCC TCCCACCCAC AATCATACCT CGCAGCCCTT CTCTAACTTT 
GGTCCTATCG GCACTCCAGA TAACCGAGAT CGTCGGGGAA CTGACCGCTG GAAGGCTGAA 
AAGCCAGTTT CAGCAATGGG TAGCTTTGGG CTGGACTACT TATCTGTGAC TCCATCTTCT 
GAGAATAGCT GGCGCCAGGC TAATAATTCA AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT 
AATGCCCGAG AGGACTCTTC AGCTGCTCTT ATGGAAAGTT TAAAGTCCAT TTGGTCAACC 
ACAATGATGC ATCCTGGACC TTCTGCCTTG GAACAGCTTC TGATGCAGCA GAAGGAGAAG 
CAGCAGCGAG GGCAGGGATC ACTGAACCCT CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816222.1
CDS	254..3832
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEEAKRSPLLPPDLLKSLAALEEEEELAFSPPPDLYPALFGPLASLPGRSLFSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960768.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960768.1

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA AGCCAAGCGT 
TCCCCTCTCC TGCCCCCGGA CCTGTTAAAG AGTCTGGCTG CTCTAGAGGA GGAGGAAGAG 
CTGGCATTCT CCCCTCCTCC TGATCTTTAC CCAGCTCTGT TCGGCCCTCT GGCCTCTCTC 
CCTGGACGAA GCCTCTTTTC TCTCCTGGAA AAGCCATCTG AACTGATATC CCAGCCCCCT 
TCTTTCCTCT CACTCTCTGG ATTTTCACTA AGCCAGGAAC GGTTCCCGAA TAACAGCATG 
TTTAATGAGG TGTATGGAAA ATCCACCTGC ACGAGCATAA AGCCCGAGGC ATGCCAAGTC 
ATGGGACAAC AGGAGACCTC CCTGTACTCT CTTTTTGAGG GTAAACCATG GTCTCCATCA 
CTTCCAGCAA GCTCAGATCA TTCTACGCCA GCCAGCCAGT CACCTCATTC TTCAAATCCA 
AGCAGCCTAC CCAGCTCTCC TCCCACCCAC AATCATACCT CGCAGCCCTT CTCTAACTTT 
GGTCCTATCG GCACTCCAGA TAACCGAGAT CGTCGGGGAA CTGACCGCTG GAAGGCTGAA 
AAGCCAGTTT CAGCAATGGG TAGCTTTGGG CTGGACTACT TATCTGTGAC TCCATCTTCT 
GAGAATAGCT GGCGCCAGGC TAATAATTCA AGTAGTGCTA ATGCTTGGGC TGCTCAAGGT 
AATGCCCGAG AGGACTCTTC AGCTGCTCTT ATGGAAAGTT TAAAGTCCAT TTGGTCAACC 
ACAATGATGC ATCCTGGACC TTCTGCCTTG GAACAGCTTC TGATGCAGCA GAAGGAGAAG 
CAGCAGCGAG GGCAGGGATC ACTGAACCCT CCGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960769.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816223.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.

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CloneID	OXa26129
Clone ID Related Accession (Same CDS sequence)	XM_012960769.2 , XM_012960769.1
Accession Version	XM_012960769.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGAA GTCTCTCCTG 
GAAAAGCCAT CTGAACTGAT ATCCCAGCCC CCTTCTTTCC TCTCACTCTC TGGATTTTCA 
CTAAGCCAGG AACGGTTCCC GAATAACAGC ATGTTTAATG AGGTGTATGG AAAATCCACC 
TGCACGAGCA TAAAGCCCGA GGCATGCCAA GTCATGGGAC AACAGGAGAC CTCCCTGTAC 
TCTCTTTTTG AGGGTAAACC ATGGTCTCCA TCACTTCCAG CAAGCTCAGA TCATTCTACG 
CCAGCCAGCC AGTCACCTCA TTCTTCAAAT CCAAGCAGCC TACCCAGCTC TCCTCCCACC 
CACAATCATA CCTCGCAGCC CTTCTCTAAC TTTGGTCCTA TCGGCACTCC AGATAACCGA 
GATCGTCGGG GAACTGACCG CTGGAAGGCT GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT 
GGGCTGGACT ACTTATCTGT GACTCCATCT TCTGAGAATA GCTGGCGCCA GGCTAATAAT 
TCAAGTAGTG CTAATGCTTG GGCTGCTCAA GGTAATGCCC GAGAGGACTC TTCAGCTGCT 
CTTATGGAAA GTTTAAAGTC CATTTGGTCA ACCACAATGA TGCATCCTGG ACCTTCTGCC 
TTGGAACAGC TTCTGATGCA GCAGAAGGAG AAGCAGCAGC GAGGGCAGGG ATCACTGAAC 
CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816223.1
CDS	254..3685
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEKSLLEKPSELISQPPSFLSLSGFSLSQERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960769.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960769.1

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGAA GTCTCTCCTG 
GAAAAGCCAT CTGAACTGAT ATCCCAGCCC CCTTCTTTCC TCTCACTCTC TGGATTTTCA 
CTAAGCCAGG AACGGTTCCC GAATAACAGC ATGTTTAATG AGGTGTATGG AAAATCCACC 
TGCACGAGCA TAAAGCCCGA GGCATGCCAA GTCATGGGAC AACAGGAGAC CTCCCTGTAC 
TCTCTTTTTG AGGGTAAACC ATGGTCTCCA TCACTTCCAG CAAGCTCAGA TCATTCTACG 
CCAGCCAGCC AGTCACCTCA TTCTTCAAAT CCAAGCAGCC TACCCAGCTC TCCTCCCACC 
CACAATCATA CCTCGCAGCC CTTCTCTAAC TTTGGTCCTA TCGGCACTCC AGATAACCGA 
GATCGTCGGG GAACTGACCG CTGGAAGGCT GAAAAGCCAG TTTCAGCAAT GGGTAGCTTT 
GGGCTGGACT ACTTATCTGT GACTCCATCT TCTGAGAATA GCTGGCGCCA GGCTAATAAT 
TCAAGTAGTG CTAATGCTTG GGCTGCTCAA GGTAATGCCC GAGAGGACTC TTCAGCTGCT 
CTTATGGAAA GTTTAAAGTC CATTTGGTCA ACCACAATGA TGCATCCTGG ACCTTCTGCC 
TTGGAACAGC TTCTGATGCA GCAGAAGGAG AAGCAGCAGC GAGGGCAGGG ATCACTGAAC 
CCTCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg7 ( XM_012960770.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012816224.1 Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa26130
Clone ID Related Accession (Same CDS sequence)	XM_012960770.2 , XM_012960770.1
Accession Version	XM_012960770.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SMG7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA ACGGTTCCCG 
AATAACAGCA TGTTTAATGA GGTGTATGGA AAATCCACCT GCACGAGCAT AAAGCCCGAG 
GCATGCCAAG TCATGGGACA ACAGGAGACC TCCCTGTACT CTCTTTTTGA GGGTAAACCA 
TGGTCTCCAT CACTTCCAGC AAGCTCAGAT CATTCTACGC CAGCCAGCCA GTCACCTCAT 
TCTTCAAATC CAAGCAGCCT ACCCAGCTCT CCTCCCACCC ACAATCATAC CTCGCAGCCC 
TTCTCTAACT TTGGTCCTAT CGGCACTCCA GATAACCGAG ATCGTCGGGG AACTGACCGC 
TGGAAGGCTG AAAAGCCAGT TTCAGCAATG GGTAGCTTTG GGCTGGACTA CTTATCTGTG 
ACTCCATCTT CTGAGAATAG CTGGCGCCAG GCTAATAATT CAAGTAGTGC TAATGCTTGG 
GCTGCTCAAG GTAATGCCCG AGAGGACTCT TCAGCTGCTC TTATGGAAAG TTTAAAGTCC 
ATTTGGTCAA CCACAATGAT GCATCCTGGA CCTTCTGCCT TGGAACAGCT TCTGATGCAG 
CAGAAGGAGA AGCAGCAGCG AGGGCAGGGA TCACTGAACC CTCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012816224.1
CDS	254..3604
Translation	MSLLCAQYLRQAEVLKADMTESKPGTAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRTNPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQMHSGTVVKPQSSSCAYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESREEVKNRWGVSDFIKAFIKFHGHVYLTKSLEKVSTLREQLEEQFKNLLFQKALNSQQLVHITVINLFQLHHLRSFGTEADQQSFSPEEELCWSQLLALFMSFLGILCRFPLKTHHCEETSCANPLPAIKVSLDWMKLRPSVFQENTVEEKKYVWPWLISLLNSFHVLDEDVSSANMLPLPEEFELQGFLALRPAFRNLDFSRGHQAITGVRDAQHRQLRPLRLAAVGKWISENQPQLIQYRTVEGELLFFTDIPELLTEDIDETEDRSSPQEFNQTDTHKAEGSPGLKSVLSTGRSLSCDTNEKPVVTFKENIKPQELNRDVPSRSYTRSGNVAKDQRDYNKSLNNAVNKKDNNNKRKNELKKMCVEKTAEPGKQSIAVQVKSQTELRKTPVSEARKTAVTPTSSPSSSTQFIPIHHPGAFPPLPSRPGFPPPAYVIPPPVAFSMSSGYSFPAGVNVPGSFLQPSSHSPSGNQGPPGKQSHIPYSQQRPSGPVTPNQGNLGNQITGQIQPQAPSNSPTQSSSQTAAQLQIQALAQQQSPTKPIQSIPKHHTSLQQYQQAETSKQVWSTSQVPNALGKMVPMKQSYYMDGRLPTEPVNMLEQCLQQQQPLENKMTSFPMEAYGQSPSDVKMPEFYWSEPPSYNLNDNRALLAQQSVNNQRSKRQPTGYRSAQEPMTRMPFEERFPNNSMFNEVYGKSTCTSIKPEACQVMGQQETSLYSLFEGKPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHTSQPFSNFGPIGTPDNRDRRGTDRWKAEKPVSAMGSFGLDYLSVTPSSENSWRQANNSSSANAWAAQGNAREDSSAALMESLKSIWSTTMMHPGPSALEQLLMQQKEKQQRGQGSLNPPH

Target ORF information:

RefSeq Version	XM_012960770.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG7 nonsense mediated mRNA decay factor (smg7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960770.1

ORF Insert Sequence:

ATGAGCCTGC TGTGTGCGCA GTATCTCAGG CAGGCTGAAG TCCTGAAAGC AGATATGACA 
GAATCTAAGC CTGGCACAGC AGAGGTGTGG ACTTCCCGTC AGGCTCTACA AGATTTGTAC 
CAGAAGATGC TGGTGACAGA CCTTGAGTAT GCACTGGACA AGAAGGTGGA ACAGGATCTC 
TGGAATCATG CCTTCAAAAA TCAGATAACA ACGTTGCAAG GGCAAGCCAA GAACCGAACT 
AATCCAAATC GCAGTGAGGT ACAGGCCAAT TTGTCACTGT TCCTTGAGGC AGCTAGTGGC 
TTCTATACAC AGCTTTTGCA GGAGCTTTGC ACTGTGTTTA ATGTGGATCT GCCATGTCGT 
GTGAAGTCCT CCCAGCTGGG CATTATAAGC AATAAGCAGA TGCACTCTGG GACAGTTGTG 
AAGCCTCAGT CCAGTTCCTG CGCCTATATC TGTCAGCATT GCTTGGTTCA TCTTGGAGAC 
ATTGCTCGCT ATAGGAACCA GACAAGCCAG GCTGAATCCT ATTATAGACA TGCTGCTCAA 
CTTGTCCCTT CCAATGGTCA GCCATATAAT CAGCTGGCTA TTCTTGCCTC TTCAAAAGGG 
GACCATTTGA CCACTATTTT CTACTACTGC CGCAGCATCG CTGTCAAGTT TCCTTTTCCA 
GCAGCCTCCA CCAATTTGCA GAAGGCCCTT TCCAAAGCCT TGGAGAGTCG CGAAGAGGTG 
AAGAATCGCT GGGGAGTATC AGACTTTATA AAAGCCTTTA TAAAGTTCCA TGGACATGTG 
TACTTAACAA AAAGTTTAGA GAAAGTAAGC ACTCTGCGGG AACAGCTGGA AGAACAATTC 
AAGAACTTAC TTTTTCAGAA AGCGCTTAAC TCCCAGCAGC TGGTTCATAT CACAGTGATA 
AACCTGTTTC AGCTACACCA TCTGCGAAGC TTTGGCACTG AGGCAGACCA ACAGAGTTTT 
AGCCCTGAAG AGGAGCTATG CTGGTCACAA CTCTTGGCTC TCTTTATGTC GTTCTTGGGC 
ATCCTCTGCC GGTTTCCCTT AAAGACCCAT CACTGTGAGG AAACCTCCTG TGCCAACCCA 
CTACCCGCTA TCAAGGTATC ATTAGACTGG ATGAAGCTGC GCCCCAGTGT CTTCCAAGAG 
AACACCGTTG AGGAAAAAAA ATATGTATGG CCATGGCTCA TTTCTCTTCT GAACAGTTTC 
CATGTGCTTG ATGAAGATGT CTCTAGTGCT AACATGCTTC CTCTCCCAGA GGAGTTTGAG 
CTGCAAGGAT TCTTGGCACT TCGACCTGCT TTTAGAAACC TAGATTTCTC TAGAGGACAC 
CAAGCCATCA CTGGAGTTAG AGATGCCCAG CACCGGCAGC TAAGACCGCT ACGACTTGCG 
GCTGTTGGAA AATGGATCTC TGAGAATCAG CCTCAGTTGA TTCAGTATCG TACTGTTGAG 
GGTGAGCTAT TGTTCTTTAC TGACATACCT GAGCTTTTGA CTGAAGACAT TGATGAGACA 
GAGGACAGAT CTTCCCCACA AGAGTTCAAC CAGACAGACA CTCATAAAGC TGAAGGCAGC 
CCTGGTTTAA AATCTGTATT ATCCACTGGC CGAAGCCTGA GCTGTGACAC AAACGAAAAG 
CCAGTGGTGA CTTTTAAAGA GAACATAAAG CCACAAGAGT TAAACCGGGA TGTACCATCA 
CGCAGCTACA CCAGATCAGG AAATGTTGCC AAGGATCAGA GAGACTATAA CAAAAGTTTA 
AATAATGCTG TCAACAAAAA GGACAATAAT AATAAAAGAA AGAATGAACT TAAGAAAATG 
TGTGTTGAGA AGACTGCTGA ACCCGGGAAG CAAAGCATTG CAGTTCAGGT GAAATCTCAA 
ACAGAGTTGA GAAAAACTCC AGTCTCTGAA GCCAGAAAAA CAGCAGTGAC TCCAACATCC 
AGTCCAAGCA GTAGCACACA GTTCATCCCT ATACATCATC CTGGTGCTTT TCCCCCTCTA 
CCCAGCCGAC CAGGTTTTCC CCCACCTGCA TATGTTATAC CACCACCTGT GGCGTTTTCC 
ATGAGCTCTG GCTATTCCTT TCCTGCTGGC GTTAATGTCC CAGGGTCTTT CCTGCAGCCT 
TCATCTCATT CACCATCTGG AAACCAGGGT CCTCCTGGGA AACAGTCCCA CATTCCTTAC 
AGTCAACAGC GCCCCTCTGG TCCAGTGACT CCTAACCAGG GTAATCTTGG CAACCAGATA 
ACTGGCCAGA TCCAACCACA GGCCCCATCC AATTCACCGA CCCAGTCCAG TAGTCAGACT 
GCAGCACAAC TACAGATCCA AGCACTTGCT CAGCAGCAGT CTCCAACTAA ACCTATACAA 
TCCATTCCCA AGCATCACAC ATCTCTCCAG CAGTACCAAC AGGCAGAAAC ATCAAAACAG 
GTCTGGAGTA CTTCTCAGGT TCCCAACGCA TTGGGAAAGA TGGTACCCAT GAAACAATCG 
TATTATATGG ATGGCCGTTT GCCAACAGAA CCTGTAAATA TGCTGGAGCA GTGTCTACAG 
CAGCAGCAGC CTCTGGAGAA TAAGATGACC TCTTTTCCCA TGGAAGCCTA TGGCCAGAGC 
CCTTCTGATG TTAAGATGCC AGAATTCTAC TGGAGTGAAC CACCATCCTA CAACTTAAAT 
GACAACAGGG CTCTCTTGGC ACAGCAATCT GTAAATAACC AAAGGAGCAA ACGCCAGCCC 
ACAGGCTACC GATCTGCCCA AGAGCCAATG ACTAGAATGC CGTTTGAGGA ACGGTTCCCG 
AATAACAGCA TGTTTAATGA GGTGTATGGA AAATCCACCT GCACGAGCAT AAAGCCCGAG 
GCATGCCAAG TCATGGGACA ACAGGAGACC TCCCTGTACT CTCTTTTTGA GGGTAAACCA 
TGGTCTCCAT CACTTCCAGC AAGCTCAGAT CATTCTACGC CAGCCAGCCA GTCACCTCAT 
TCTTCAAATC CAAGCAGCCT ACCCAGCTCT CCTCCCACCC ACAATCATAC CTCGCAGCCC 
TTCTCTAACT TTGGTCCTAT CGGCACTCCA GATAACCGAG ATCGTCGGGG AACTGACCGC 
TGGAAGGCTG AAAAGCCAGT TTCAGCAATG GGTAGCTTTG GGCTGGACTA CTTATCTGTG 
ACTCCATCTT CTGAGAATAG CTGGCGCCAG GCTAATAATT CAAGTAGTGC TAATGCTTGG 
GCTGCTCAAG GTAATGCCCG AGAGGACTCT TCAGCTGCTC TTATGGAAAG TTTAAAGTCC 
ATTTGGTCAA CCACAATGAT GCATCCTGGA CCTTCTGCCT TGGAACAGCT TCTGATGCAG 
CAGAAGGAGA AGCAGCAGCG AGGGCAGGGA TCACTGAACC CTCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	smg7
Entrez Gene ID	394870
Full Name	SMG7 nonsense mediated mRNA decay factor
General protein information	Preferred Names SMG7 nonsense mediated mRNA decay factor Names protein SMG7 smg-7 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	SMG7	XP_002802068.1
Mus musculus (house mouse)	Smg7	NP_001005507.1
Danio rerio (zebrafish)	smg7	NP_001038548.2
Bos taurus (cattle)	SMG7	NP_001180134.2
Xenopus tropicalis (tropical clawed frog)	smg7	NP_989258.1
Homo sapiens (human)	SMG7	NP_775179.1
Pan troglodytes (chimpanzee)	SMG7	XP_514048.3
Gallus gallus (chicken)	SMG7	XP_004943329.1
Canis lupus familiaris (dog)	SMG7	XP_537157.4
Rattus norvegicus (Norway rat)	Smg7	NP_001178478.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

smg7 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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