znf521 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following znf521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa11599 XM_004915209.2
Latest version!
Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa11600 XM_012965300.2
Latest version!
Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa37703 XM_012965302.3
Latest version!
Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OXa37703 XM_012965302.2 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa11600 XM_012965300.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa11599 XM_004915209.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa11599
    Clone ID Related Accession (Same CDS sequence) XM_004915209.2 , XM_004915209.1
    Accession Version XM_004915209.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915266.1
    CDS202..4140
    Translation

    Target ORF information:

    RefSeq Version XM_004915209.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915209.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11600
    Clone ID Related Accession (Same CDS sequence) XM_012965300.1 , XM_012965300.2
    Accession Version XM_012965300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC
    CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT
    GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA
    CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC
    TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA
    CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG
    CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820754.1
    CDS202..4137
    Translation

    Target ORF information:

    RefSeq Version XM_012965300.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC
    CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT
    GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA
    CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC
    TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA
    CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG
    CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37703
    Clone ID Related Accession (Same CDS sequence) XM_012965302.3 , XM_012965302.2
    Accession Version XM_012965302.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820756.1
    CDS851..4129
    Translation

    Target ORF information:

    RefSeq Version XM_012965302.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965302.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11599
    Clone ID Related Accession (Same CDS sequence) XM_004915209.2 , XM_004915209.1
    Accession Version XM_004915209.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915266.1
    CDS203..4141
    Translation

    Target ORF information:

    RefSeq Version XM_004915209.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11600
    Clone ID Related Accession (Same CDS sequence) XM_012965300.1 , XM_012965300.2
    Accession Version XM_012965300.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC
    CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT
    GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA
    CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC
    TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA
    CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG
    CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820754.1
    CDS203..4138
    Translation

    Target ORF information:

    RefSeq Version XM_012965300.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
    GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG
    CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC
    GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT
    GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC
    CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC
    CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC
    AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT
    AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT
    GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG
    CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC
    CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT
    GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA
    CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC
    TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA
    CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG
    CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37703
    Clone ID Related Accession (Same CDS sequence) XM_012965302.3 , XM_012965302.2
    Accession Version XM_012965302.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product zinc finger protein 521 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820756.1
    CDS861..4139
    Translation

    Target ORF information:

    RefSeq Version XM_012965302.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
    AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT
    CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC
    CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG
    GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT
    AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC
    AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT
    TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC
    CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA
    GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT
    TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG
    TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC
    GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC
    TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT
    GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG
    GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC
    AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT
    TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC
    ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA
    TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG
    CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT
    GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT
    TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA
    AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC
    AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG
    TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC
    TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC
    TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA
    AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT
    CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA
    ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA
    AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG
    CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT
    ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT
    CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG
    AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT
    TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG
    TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC
    CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG
    GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT
    CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG
    TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC
    TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT
    GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC
    AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA
    ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA
    TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA
    ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA
    CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG
    TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT
    GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG
    CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT
    GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC
    GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA
    GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.