tjp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tjp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tjp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43354 XM_018092414.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa55060 XM_031899168.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa55052 XM_031899160.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55053 XM_031899161.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55054 XM_031899162.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55055 XM_031899163.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55056 XM_031899164.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55057 XM_031899165.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55058 XM_031899166.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55059 XM_031899167.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa55061 XM_031899169.1
Latest version!
Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43354
    Clone ID Related Accession (Same CDS sequence) XM_018092414.1
    Accession Version XM_018092414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCGGC CAAGCATGAA ACTGGTGAAG TTCAGGAAAG GAGACAGTGT AGGCCTTCGT 
    TTGGCTGGTG GTAATGATGT GGGAATTTTT GTTGCTGGTG TATTAGACGA CAGTCCTGCC
    GCAAAAGAAG GTTTGGAGGA AGGGGACCAG ATACTCAGAG TTAACAATGT GGATTTCACC
    AATATAATAA GGGAAGAAGC GGTACTGTTC CTTCTTGATC TGCCCAAAGG AGAAGAGGTT
    ACCATTCTTG CTCAGAAAAA GAAAGATGTG TATCGCCGCA TTGTTGAGTC AGATGTTGGG
    GATTCATTTT ATATAAGAAC TCACTTTGAA TATGAGAAAG AGTCACCATA CGGGCTAAGT
    TTCAACAAAG GAGAGGTCTT TCGAGTTGTG GATACTCTTT ACAATGGCAA ACTAGGGTCA
    TGGTTGGCCA TTAGAATTGG TAGAAATCAC AAGGAGGTGG AAAGAGGGAT AATCCCCAAC
    AAGAATAGGG CTGAGCAACT GGCTAGTGTA CAGTACACTC TTCCTAAAAC TGCTGGTGGA
    GATCGTGCAG ACTTCTGGAG ATTCCGTGGC CTTCGTAGTT CCAAGCGAAA TCTACGGAAA
    AGCAGAGAGG ATCTTTCTGC GCAACCTGTT CAAACAAAGT TCCCTGCTTA TGAGAGAGTC
    GTCTTGAGAG AAGCTGGATT TCTCAGGCCT GTGGTTATTT TTGGCCCTAT TGCTGACGTT
    GCAAGAGAAA AGTTGGCAAG AGAAGAACCA GATATTTATG AAGTAGCAAG AAGTGAACCC
    AGAGATGCAG GTACTGACCA GCGAAGTTCT GGAATTATCC GCCTTCATAC TATTAAACAG
    ATTATTGATA GGGATAAGCA TGCTCTTCTA GATGTGACTC CAAATGCTGT GGACCGCTTA
    AATTATGCAC AATGGTATCC GATAGTAGTG TTTCTTAACC CAGACTCTAA ACAAGGTGTG
    AAAACAATGA GAATGAGGTT ATGCCCAGAA TCACGCAAAA GTGCCAGGAA ACTATATGAA
    CGTTCACACA AACTACGCAA AAACAATCAC CATCTTTTCA CAACTACAAT AAATCTCAAC
    TCCATGAATG ATGGCTGGTA TGGTGGCCTT AAAGAGGCAG CACAGCAGCA ACAGAACCAG
    CTAGTTTGGG TCTCAGAGGG CAAAGCAGAA GGTGGCACAG ATGATGACCT TGATTTGCAT
    GACGACCGTC TTTCCTACCT ATCAGCACCA GGTAGTGAAT ATTCAATGTA TAGCACTGAT
    AGTAGGCACA CATCAGACTA TGAAGACACA GACACAGAAG GAGGGGCTTA CACTGACCAG
    GACCTGGATG AGACACTTAA TGATGAGGCT GGGACTCCAC CAGAATCTGC AATCACACGA
    TCTTCTGAAC CAGTCAGAGA AGACACATCA GGGATGCAAG AAAACCCAGC ATACCCAACA
    TATCCACCTC AGCCAATCCT TAGAACAGAT TCTCCTGGGT ATAAAAATGT ACCTTCTCAA
    CAGAAAGCAG AAGCCTTACC TGCAGCCCCT TACCTTTCTG TGTCGTCTGA AACAAACCAT
    ACAACTTCAA CTGCTGCTGT TAATCCCATG GGTGGCCTTT TGAGATCACC AAGCACTGAG
    GCAGCTCATA TTATACTAAG AGACCAAGAA CCTTCATCTT TACCAGTGCA AATTGACTCA
    GCAAAGGTTT ATAGAAAAGA GGCCTATGCT ATGGAAGAGA ATCCTAGACA AAATTATTTG
    ATGAAGCAGC AAGTGCTTGG CCATCAAATT CAAAGACATG AAAAAGAGAC CAATTTAAAC
    TATGATGGCG TGCCAGCATA TGCAGAGAAA CCACTGAATA GAGAATATGA CCAGTCTTAT
    TGCTATGATT CTACTTCTGA TTATGTAGAT CAGTTTCCCC ACAATTATGA TTCTCGAATA
    AATTTTGATG ATCACGTGCC TACATATGAT GAACAGTGGG GTTATTATGA TGACAAACAG
    TCATATCCCC AGAGACCACC ATTTGACAGT AAACGCAATA GGGACTTCAA TATTAGACAG
    CACTCTGATG AAAACCTTGA ACGTAATTAC TTTCAGCAGC CTCGATTGGA GGAGCCACCT
    CCTGTTGCTT ATGACAATCG CCCCCGCCCA TCCCAAAACT ACAAACCAAC CCAAGTACAT
    TATGAAGACT CCACAACAGC AGAGTATGAT ATGCATATGC GTTACAAACC TGAACCTCAA
    ACCATTCCTT CTGTGCCAAC ATCAAAGTCT TCTGATGCAA AGCCGTATTT TGATCAACAC
    ATAAGGGGTT ATGAACATAG TCCTACCCAG GTATTTAACA CAAAACCAGG GCAGTATGAT
    ACATCTCCTA GAATGGAGGC AATGCCACCT CCACCTGTTC AGAGTAAGCC AGAGCTCCTT
    TCAACAAGCT CAAAACCTTT GCCTGCCCCT GCTCCTTCCG TTGCTCCTGC AGCAACTGAA
    GAAGACGATG ATCCAGCTAT GAAGCCACAG TCTGTACTTA CTAGAGTGAA AATGTTTGAG
    AACAAACGTT CTAATCAAGG TGAAAAGAAC AAGGATATAA ATGATTCCAC TCTTGTTAAG
    GCCCCAGATG TTGCACCTAA ACCTATACTT AATCAAGTAA GTGCTACAAA GGCTGCCCCC
    GTTCCACATT ATGATCAGGA CAAGGCAACT TATAGAGTAC CTGAACCTCA AAAGCCTCAA
    GTGAAGCCCC CAGAAGATAT AATTCGGGCC AACCACTATG ACCCCGATGA AGATGAAGAA
    TACTATCGAA AGCAGCTGTC TTACTTTGAT CGAAGAAGCT TTGAAAAGCC CACACCCTTG
    CTGTCTGGAA ACATCCTGCC TGAGCCTGCC AAACCAGCGC AGTCTCAAAG CCAACTTAGC
    TTTAACAGCT ACCCCTCCAA GGGAAAGCCT GCAGATTTGG AGCCACTTGA CAGACCCATT
    AATGAAAAAC GCTATGATCC AGTTCCACAG GTTACTTCTT CTAACCAGTA TACTCAGCCT
    TCACCAGTGG TTGTAAATCA TTCAACACAC GCTAAACATA TGCATTTAGA AGCCAGCTCT
    GTGTCTGTGG AGGTTCCAAG TTCCACAGTG TCTAAGCCAG AGCCCCCACC TACTCAGGCC
    AAGCCAACAT TGTTCCGACC ATCAAACAGA GAGGACACTG TACAGTCAAA CTTCTATCCT
    CAGAAGAGTT TCCCTGAAAA AAGCCCTGTA AATGGCACTG AACAGGCTCC AAAAACAGTA
    ACACCATCCT ACAATCGTTT TACACCTAAA CCTTACACAA ATTCAGCGAG ACCCTTTGAA
    CGCAAGTTTG AAAGCCCAAA ATTTAACCAC AACCTCCTGC CAAATGAAGC ACAAGTAAAG
    CCAGAGCTGC CCACTAAACC TCAGAATTCT CCACAGCCTG TTTTAAAATC AAATACGTCA
    ATTTCTTCTG AACTGGATAG CATATCCAAG CAAACGGAGA ACAAATCTAA ATATCAACCA
    AACAATGTTA ATCCAGTGCC TAAAGCAATC CCAGTCAGTC CTTCTGCATT GGAAGATGAC
    GATGAAGATG ATGCACATAC AGTTGTAGCA ACAGCTAGAG GCTTCTTTAA TAGTAATGGA
    GGTGTGTTAA GTTCCATAGA AACGGGAGTC AGTATCATCA TACCCCAAGG AGCAATTCCA
    GATGGCATTG AGCAAGAAAT ATATTTCAAG GTCTGCAGGG ATAACAGTAT CCTTCCACCC
    TTGGACAAAG AGAAAGGAGA GACGCTACTT AGTCCATTAG TAATGTGCGG ACCTCATGGG
    TTAAAATTCG TGAAACCAGT GGAGCTACGC TTACCACACT GTGCGTCTAT GACTCCTGAT
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC ACCTCGTCGG GTGACCCAAA ACACTGGCAG
    AACAAGTCAC TTCCTGGCGA CCCCAACTAT CTTGTCGGAG CAAACTGTGT CTCAGTTTTG
    ATAGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947903.1
    CDS102..4076
    Translation

    Target ORF information:

    RefSeq Version XM_018092414.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTTCGGC CAAGCATGAA ACTGGTGAAG TTCAGGAAAG GAGACAGTGT AGGCCTTCGT 
    TTGGCTGGTG GTAATGATGT GGGAATTTTT GTTGCTGGTG TATTAGACGA CAGTCCTGCC
    GCAAAAGAAG GTTTGGAGGA AGGGGACCAG ATACTCAGAG TTAACAATGT GGATTTCACC
    AATATAATAA GGGAAGAAGC GGTACTGTTC CTTCTTGATC TGCCCAAAGG AGAAGAGGTT
    ACCATTCTTG CTCAGAAAAA GAAAGATGTG TATCGCCGCA TTGTTGAGTC AGATGTTGGG
    GATTCATTTT ATATAAGAAC TCACTTTGAA TATGAGAAAG AGTCACCATA CGGGCTAAGT
    TTCAACAAAG GAGAGGTCTT TCGAGTTGTG GATACTCTTT ACAATGGCAA ACTAGGGTCA
    TGGTTGGCCA TTAGAATTGG TAGAAATCAC AAGGAGGTGG AAAGAGGGAT AATCCCCAAC
    AAGAATAGGG CTGAGCAACT GGCTAGTGTA CAGTACACTC TTCCTAAAAC TGCTGGTGGA
    GATCGTGCAG ACTTCTGGAG ATTCCGTGGC CTTCGTAGTT CCAAGCGAAA TCTACGGAAA
    AGCAGAGAGG ATCTTTCTGC GCAACCTGTT CAAACAAAGT TCCCTGCTTA TGAGAGAGTC
    GTCTTGAGAG AAGCTGGATT TCTCAGGCCT GTGGTTATTT TTGGCCCTAT TGCTGACGTT
    GCAAGAGAAA AGTTGGCAAG AGAAGAACCA GATATTTATG AAGTAGCAAG AAGTGAACCC
    AGAGATGCAG GTACTGACCA GCGAAGTTCT GGAATTATCC GCCTTCATAC TATTAAACAG
    ATTATTGATA GGGATAAGCA TGCTCTTCTA GATGTGACTC CAAATGCTGT GGACCGCTTA
    AATTATGCAC AATGGTATCC GATAGTAGTG TTTCTTAACC CAGACTCTAA ACAAGGTGTG
    AAAACAATGA GAATGAGGTT ATGCCCAGAA TCACGCAAAA GTGCCAGGAA ACTATATGAA
    CGTTCACACA AACTACGCAA AAACAATCAC CATCTTTTCA CAACTACAAT AAATCTCAAC
    TCCATGAATG ATGGCTGGTA TGGTGGCCTT AAAGAGGCAG CACAGCAGCA ACAGAACCAG
    CTAGTTTGGG TCTCAGAGGG CAAAGCAGAA GGTGGCACAG ATGATGACCT TGATTTGCAT
    GACGACCGTC TTTCCTACCT ATCAGCACCA GGTAGTGAAT ATTCAATGTA TAGCACTGAT
    AGTAGGCACA CATCAGACTA TGAAGACACA GACACAGAAG GAGGGGCTTA CACTGACCAG
    GACCTGGATG AGACACTTAA TGATGAGGCT GGGACTCCAC CAGAATCTGC AATCACACGA
    TCTTCTGAAC CAGTCAGAGA AGACACATCA GGGATGCAAG AAAACCCAGC ATACCCAACA
    TATCCACCTC AGCCAATCCT TAGAACAGAT TCTCCTGGGT ATAAAAATGT ACCTTCTCAA
    CAGAAAGCAG AAGCCTTACC TGCAGCCCCT TACCTTTCTG TGTCGTCTGA AACAAACCAT
    ACAACTTCAA CTGCTGCTGT TAATCCCATG GGTGGCCTTT TGAGATCACC AAGCACTGAG
    GCAGCTCATA TTATACTAAG AGACCAAGAA CCTTCATCTT TACCAGTGCA AATTGACTCA
    GCAAAGGTTT ATAGAAAAGA GGCCTATGCT ATGGAAGAGA ATCCTAGACA AAATTATTTG
    ATGAAGCAGC AAGTGCTTGG CCATCAAATT CAAAGACATG AAAAAGAGAC CAATTTAAAC
    TATGATGGCG TGCCAGCATA TGCAGAGAAA CCACTGAATA GAGAATATGA CCAGTCTTAT
    TGCTATGATT CTACTTCTGA TTATGTAGAT CAGTTTCCCC ACAATTATGA TTCTCGAATA
    AATTTTGATG ATCACGTGCC TACATATGAT GAACAGTGGG GTTATTATGA TGACAAACAG
    TCATATCCCC AGAGACCACC ATTTGACAGT AAACGCAATA GGGACTTCAA TATTAGACAG
    CACTCTGATG AAAACCTTGA ACGTAATTAC TTTCAGCAGC CTCGATTGGA GGAGCCACCT
    CCTGTTGCTT ATGACAATCG CCCCCGCCCA TCCCAAAACT ACAAACCAAC CCAAGTACAT
    TATGAAGACT CCACAACAGC AGAGTATGAT ATGCATATGC GTTACAAACC TGAACCTCAA
    ACCATTCCTT CTGTGCCAAC ATCAAAGTCT TCTGATGCAA AGCCGTATTT TGATCAACAC
    ATAAGGGGTT ATGAACATAG TCCTACCCAG GTATTTAACA CAAAACCAGG GCAGTATGAT
    ACATCTCCTA GAATGGAGGC AATGCCACCT CCACCTGTTC AGAGTAAGCC AGAGCTCCTT
    TCAACAAGCT CAAAACCTTT GCCTGCCCCT GCTCCTTCCG TTGCTCCTGC AGCAACTGAA
    GAAGACGATG ATCCAGCTAT GAAGCCACAG TCTGTACTTA CTAGAGTGAA AATGTTTGAG
    AACAAACGTT CTAATCAAGG TGAAAAGAAC AAGGATATAA ATGATTCCAC TCTTGTTAAG
    GCCCCAGATG TTGCACCTAA ACCTATACTT AATCAAGTAA GTGCTACAAA GGCTGCCCCC
    GTTCCACATT ATGATCAGGA CAAGGCAACT TATAGAGTAC CTGAACCTCA AAAGCCTCAA
    GTGAAGCCCC CAGAAGATAT AATTCGGGCC AACCACTATG ACCCCGATGA AGATGAAGAA
    TACTATCGAA AGCAGCTGTC TTACTTTGAT CGAAGAAGCT TTGAAAAGCC CACACCCTTG
    CTGTCTGGAA ACATCCTGCC TGAGCCTGCC AAACCAGCGC AGTCTCAAAG CCAACTTAGC
    TTTAACAGCT ACCCCTCCAA GGGAAAGCCT GCAGATTTGG AGCCACTTGA CAGACCCATT
    AATGAAAAAC GCTATGATCC AGTTCCACAG GTTACTTCTT CTAACCAGTA TACTCAGCCT
    TCACCAGTGG TTGTAAATCA TTCAACACAC GCTAAACATA TGCATTTAGA AGCCAGCTCT
    GTGTCTGTGG AGGTTCCAAG TTCCACAGTG TCTAAGCCAG AGCCCCCACC TACTCAGGCC
    AAGCCAACAT TGTTCCGACC ATCAAACAGA GAGGACACTG TACAGTCAAA CTTCTATCCT
    CAGAAGAGTT TCCCTGAAAA AAGCCCTGTA AATGGCACTG AACAGGCTCC AAAAACAGTA
    ACACCATCCT ACAATCGTTT TACACCTAAA CCTTACACAA ATTCAGCGAG ACCCTTTGAA
    CGCAAGTTTG AAAGCCCAAA ATTTAACCAC AACCTCCTGC CAAATGAAGC ACAAGTAAAG
    CCAGAGCTGC CCACTAAACC TCAGAATTCT CCACAGCCTG TTTTAAAATC AAATACGTCA
    ATTTCTTCTG AACTGGATAG CATATCCAAG CAAACGGAGA ACAAATCTAA ATATCAACCA
    AACAATGTTA ATCCAGTGCC TAAAGCAATC CCAGTCAGTC CTTCTGCATT GGAAGATGAC
    GATGAAGATG ATGCACATAC AGTTGTAGCA ACAGCTAGAG GCTTCTTTAA TAGTAATGGA
    GGTGTGTTAA GTTCCATAGA AACGGGAGTC AGTATCATCA TACCCCAAGG AGCAATTCCA
    GATGGCATTG AGCAAGAAAT ATATTTCAAG GTCTGCAGGG ATAACAGTAT CCTTCCACCC
    TTGGACAAAG AGAAAGGAGA GACGCTACTT AGTCCATTAG TAATGTGCGG ACCTCATGGG
    TTAAAATTCG TGAAACCAGT GGAGCTACGC TTACCACACT GTGCGTCTAT GACTCCTGAT
    GGTTGGTCTT TTGCTCTAAA ATCATCCGAC ACCTCGTCGG GTGACCCAAA ACACTGGCAG
    AACAAGTCAC TTCCTGGCGA CCCCAACTAT CTTGTCGGAG CAAACTGTGT CTCAGTTTTG
    ATAGACCACT TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55060
    Clone ID Related Accession (Same CDS sequence) XM_031899168.1
    Accession Version XM_031899168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGCCCC AGATGTTGCA CCTAAACCTA TACTTAATCA AGTAAGTGCT
    ACAAAGGCTG CCCCCGTTCC ACATTATGAT CAGGACAAGG CAACTTATAG AGTACCTGAA
    CCTCAAAAGC CTCAAGTGAA GCCCCCAGAA GATATAATTC GGGCCAACCA CTATGACCCC
    GATGAAGATG AAGAATACTA TCGAAAGCAG CTGTCTTACT TTGATCGAAG AAGCTTTGAA
    AAGCCCACAC CCTTGCTGTC TGGAAACATC CTGCCTGAGC CTGCCAAACC AGCGCAGTCT
    CAAAGCCAAC TTAGCTTTAA CAGCTACCCC TCCAAGGGAA AGCCTGCAGA TTTGGAGCCA
    CTTGACAGAC CCATTAATGA AAAACGCTAT GATCCAGTTC CACAGGTTAC TTCTTCTAAC
    CAGTATACTC AGCCTTCACC AGTGGTTGTA AATCATTCAA CACACACTAA ACATATGCAT
    TTAGAAGCCA GCTCTGTGTC TGTGGAGGTT CCAAGTTCCA CAGTGTCTAA GCCAGAGCCC
    CCACCTACTC AGGCCAAGCC AACATTGTTC CGACCATCAA ACAGAGAGGA CACTGTACAG
    TCAAACTTCT ATCCTCAGAA GAGTTTCCCT GAAAAAAGCC CTGTAAATGG CACTGAACAG
    GCTCCAAAAA CAGTAACACC ATCCTACAAT CGTTTTACAC CTAAACCTTA CACAAATTCA
    GCGAGACCCT TTGAACGCAA GTTTGAAAGC CCAAAATTTA ACCACAACCT CCTGCCAAAT
    GAAGCACAAG TAAAGCCAGA GCTGCCCACT AAACCTCAGA ATTCTCCACA GCCTGTTTTA
    AAATCAAATA CGTCAATTTC TTCTGAACTG GATAGCATAT CCAAGCAAAC GGAGAACAAA
    TCTAAATATC AACCAAACAA TGTTAATCCA GTGCCTAAAG CAATCCCAGT CAGTCCTTCT
    GCATTGGAAG ATGACGATGA AGATGATGCA CATACAGTTG TAGCAACAGC TAGAGGCTTC
    TTTAATAGTA ATGGAGGTGT GTTAAGTTCC ATAGAAACGG GAGTCAGTAT CATCATACCC
    CAAGGAGCAA TTCCAGATGG CATTGAGCAA GAAATATATT TCAAGGTCTG CAGGGATAAC
    AGTATCCTTC CACCCTTGGA CAAAGAGAAA GGAGAGACGC TACTTAGTCC ATTAGTAATG
    TGCGGACCTC ATGGGTTAAA ATTCGTGAAA CCAGTGGAGC TACGCTTACC ACACTGTGCG
    TCTATGACTC CTGATGGTTG GTCTTTTGCT CTAAAATCAT CCGACACCTC GTCGGGTGAC
    CCAAAACACT GGCAGAACAA GTCACTTCCT GGCGACCCCA ACTATCTTGT CGGAGCAAAC
    TGTGTCTCAG TTTTGATAGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755028.1
    CDS25..4374
    Translation

    Target ORF information:

    RefSeq Version XM_031899168.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGCCCC AGATGTTGCA CCTAAACCTA TACTTAATCA AGTAAGTGCT
    ACAAAGGCTG CCCCCGTTCC ACATTATGAT CAGGACAAGG CAACTTATAG AGTACCTGAA
    CCTCAAAAGC CTCAAGTGAA GCCCCCAGAA GATATAATTC GGGCCAACCA CTATGACCCC
    GATGAAGATG AAGAATACTA TCGAAAGCAG CTGTCTTACT TTGATCGAAG AAGCTTTGAA
    AAGCCCACAC CCTTGCTGTC TGGAAACATC CTGCCTGAGC CTGCCAAACC AGCGCAGTCT
    CAAAGCCAAC TTAGCTTTAA CAGCTACCCC TCCAAGGGAA AGCCTGCAGA TTTGGAGCCA
    CTTGACAGAC CCATTAATGA AAAACGCTAT GATCCAGTTC CACAGGTTAC TTCTTCTAAC
    CAGTATACTC AGCCTTCACC AGTGGTTGTA AATCATTCAA CACACACTAA ACATATGCAT
    TTAGAAGCCA GCTCTGTGTC TGTGGAGGTT CCAAGTTCCA CAGTGTCTAA GCCAGAGCCC
    CCACCTACTC AGGCCAAGCC AACATTGTTC CGACCATCAA ACAGAGAGGA CACTGTACAG
    TCAAACTTCT ATCCTCAGAA GAGTTTCCCT GAAAAAAGCC CTGTAAATGG CACTGAACAG
    GCTCCAAAAA CAGTAACACC ATCCTACAAT CGTTTTACAC CTAAACCTTA CACAAATTCA
    GCGAGACCCT TTGAACGCAA GTTTGAAAGC CCAAAATTTA ACCACAACCT CCTGCCAAAT
    GAAGCACAAG TAAAGCCAGA GCTGCCCACT AAACCTCAGA ATTCTCCACA GCCTGTTTTA
    AAATCAAATA CGTCAATTTC TTCTGAACTG GATAGCATAT CCAAGCAAAC GGAGAACAAA
    TCTAAATATC AACCAAACAA TGTTAATCCA GTGCCTAAAG CAATCCCAGT CAGTCCTTCT
    GCATTGGAAG ATGACGATGA AGATGATGCA CATACAGTTG TAGCAACAGC TAGAGGCTTC
    TTTAATAGTA ATGGAGGTGT GTTAAGTTCC ATAGAAACGG GAGTCAGTAT CATCATACCC
    CAAGGAGCAA TTCCAGATGG CATTGAGCAA GAAATATATT TCAAGGTCTG CAGGGATAAC
    AGTATCCTTC CACCCTTGGA CAAAGAGAAA GGAGAGACGC TACTTAGTCC ATTAGTAATG
    TGCGGACCTC ATGGGTTAAA ATTCGTGAAA CCAGTGGAGC TACGCTTACC ACACTGTGCG
    TCTATGACTC CTGATGGTTG GTCTTTTGCT CTAAAATCAT CCGACACCTC GTCGGGTGAC
    CCAAAACACT GGCAGAACAA GTCACTTCCT GGCGACCCCA ACTATCTTGT CGGAGCAAAC
    TGTGTCTCAG TTTTGATAGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55052
    Clone ID Related Accession (Same CDS sequence) XM_031899160.1
    Accession Version XM_031899160.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GCGTCTATGA CTCCTGATGG TTGGTCTTTT GCTCTAAAAT CATCCGACAC CTCGTCGGGT
    GACCCAAAAC ACTGGCAGAA CAAGTCACTT CCTGGCGACC CCAACTATCT TGTCGGAGCA
    AACTGTGTCT CAGTTTTGAT AGACCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755020.1
    CDS25..5517
    Translation

    Target ORF information:

    RefSeq Version XM_031899160.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GCGTCTATGA CTCCTGATGG TTGGTCTTTT GCTCTAAAAT CATCCGACAC CTCGTCGGGT
    GACCCAAAAC ACTGGCAGAA CAAGTCACTT CCTGGCGACC CCAACTATCT TGTCGGAGCA
    AACTGTGTCT CAGTTTTGAT AGACCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55053
    Clone ID Related Accession (Same CDS sequence) XM_031899161.1
    Accession Version XM_031899161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GCGTCTATGA CTCCTGATGG TGACCCAAAA CACTGGCAGA ACAAGTCACT TCCTGGCGAC
    CCCAACTATC TTGTCGGAGC AAACTGTGTC TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755021.1
    CDS25..5478
    Translation

    Target ORF information:

    RefSeq Version XM_031899161.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GCGTCTATGA CTCCTGATGG TGACCCAAAA CACTGGCAGA ACAAGTCACT TCCTGGCGAC
    CCCAACTATC TTGTCGGAGC AAACTGTGTC TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55054
    Clone ID Related Accession (Same CDS sequence) XM_031899162.1
    Accession Version XM_031899162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GACCCAAAAC ACTGGCAGAA CAAGTCACTT CCTGGCGACC CCAACTATCT TGTCGGAGCA
    AACTGTGTCT CAGTTTTGAT AGACCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755022.1
    CDS25..5457
    Translation

    Target ORF information:

    RefSeq Version XM_031899162.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTAAATCT AAATAATGTT
    CGATTGGAGG ATCCCAACCA TGCCCCTTAC AGCTCTTATC AATCACAGGG TGGCCTTTTG
    AGATCACCAA GCACTGAGGC AGCTCATATT ATACTAAGAG ACCAAGAACC TTCATCTTTA
    CCAGTGCAAA TTGACTCAGC AAAGGTTTAT AGAAAAGAGG CCTATGCTAT GGAAGAGAAT
    CCTAGACAAA ATTATTTGAT GAAGCAGCAA GTGCTTGGCC ATCAAATTCA AAGACATGAA
    AAAGAGACCA ATTTAAACTA TGATGGCGTG CCAGCATATG CAGAGAAACC ACTGAATAGA
    GAATATGACC AGTCTTATTG CTATGATTCT ACTTCTGATT ATGTAGATCA GTTTCCCCAC
    AATTATGATT CTCGAATAAA TTTTGATGAT CACGTGCCTA CATATGATGA ACAGTGGGGT
    TATTATGATG ACAAACAGTC ATATCCCCAG AGACCACCAT TTGACAGTAA ACGCAATAGG
    GACTTCAATA TTAGACAGCA CTCTGATGAA AACCTTGAAC GTAATTACTT TCAGCAGCCT
    CGATTGGAGG AGCCACCTCC TGTTGCTTAT GACAATCGCC CCCGCCCATC CCAAAACTAC
    AAACCAACCC AAGTACATTA TGAAGACTCC ACAACAGCAG AGTATGATAT GCATATGCGT
    TACAAACCTG AACCTCAAAC CATTCCTTCT GTGCCAACAT CAAAGTCTTC TGATGCAAAG
    CCGTATTTTG ATCAACACAT AAGGGGTTAT GAACATAGTC CTACCCAGGT ATTTAACACA
    AAACCAGGGC AGTATGATAC ATCTCCTAGA ATGGAGGCAA TGCCACCTCC ACCTGTTCAG
    AGTAAGCCAG AGCTCCTTTC AACAAGCTCA AAACCTTTGC CTGCCCCTGC TCCTTCCGTT
    GCTCCTGCAG CAACTGAAGA AGACGATGAT CCAGCTATGA AGCCACAGTC TGTACTTACT
    AGAGTGAAAA TGTTTGAGAA CAAACGTTCT AATCAAGGTG AAAAGAACAA GGATATAAAT
    GATTCCACTC TTGTTAAGGC CCCAGATGTT GCACCTAAAC CTATACTTAA TCAAGTAAGT
    GCTACAAAGG CTGCCCCCGT TCCACATTAT GATCAGGACA AGGCAACTTA TAGAGTACCT
    GAACCTCAAA AGCCTCAAGT GAAGCCCCCA GAAGATATAA TTCGGGCCAA CCACTATGAC
    CCCGATGAAG ATGAAGAATA CTATCGAAAG CAGCTGTCTT ACTTTGATCG AAGAAGCTTT
    GAAAAGCCCA CACCCTTGCT GTCTGGAAAC ATCCTGCCTG AGCCTGCCAA ACCAGCGCAG
    TCTCAAAGCC AACTTAGCTT TAACAGCTAC CCCTCCAAGG GAAAGCCTGC AGATTTGGAG
    CCACTTGACA GACCCATTAA TGAAAAACGC TATGATCCAG TTCCACAGGT TACTTCTTCT
    AACCAGTATA CTCAGCCTTC ACCAGTGGTT GTAAATCATT CAACACACAC TAAACATATG
    CATTTAGAAG CCAGCTCTGT GTCTGTGGAG GTTCCAAGTT CCACAGTGTC TAAGCCAGAG
    CCCCCACCTA CTCAGGCCAA GCCAACATTG TTCCGACCAT CAAACAGAGA GGACACTGTA
    CAGTCAAACT TCTATCCTCA GAAGAGTTTC CCTGAAAAAA GCCCTGTAAA TGGCACTGAA
    CAGGCTCCAA AAACAGTAAC ACCATCCTAC AATCGTTTTA CACCTAAACC TTACACAAAT
    TCAGCGAGAC CCTTTGAACG CAAGTTTGAA AGCCCAAAAT TTAACCACAA CCTCCTGCCA
    AATGAAGCAC AAGTAAAGCC AGAGCTGCCC ACTAAACCTC AGAATTCTCC ACAGCCTGTT
    TTAAAATCAA ATACGTCAAT TTCTTCTGAA CTGGATAGCA TATCCAAGCA AACGGAGAAC
    AAATCTAAAT ATCAACCAAA CAATGTTAAT CCAGTGCCTA AAGCAATCCC AGTCAGTCCT
    TCTGCATTGG AAGATGACGA TGAAGATGAT GCACATACAG TTGTAGCAAC AGCTAGAGGC
    TTCTTTAATA GTAATGGAGG TGTGTTAAGT TCCATAGAAA CGGGAGTCAG TATCATCATA
    CCCCAAGGAG CAATTCCAGA TGGCATTGAG CAAGAAATAT ATTTCAAGGT CTGCAGGGAT
    AACAGTATCC TTCCACCCTT GGACAAAGAG AAAGGAGAGA CGCTACTTAG TCCATTAGTA
    ATGTGCGGAC CTCATGGGTT AAAATTCGTG AAACCAGTGG AGCTACGCTT ACCACACTGT
    GACCCAAAAC ACTGGCAGAA CAAGTCACTT CCTGGCGACC CCAACTATCT TGTCGGAGCA
    AACTGTGTCT CAGTTTTGAT AGACCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55055
    Clone ID Related Accession (Same CDS sequence) XM_031899163.1
    Accession Version XM_031899163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGGTGGCCT TTTGAGATCA
    CCAAGCACTG AGGCAGCTCA TATTATACTA AGAGACCAAG AACCTTCATC TTTACCAGTG
    CAAATTGACT CAGCAAAGGT TTATAGAAAA GAGGCCTATG CTATGGAAGA GAATCCTAGA
    CAAAATTATT TGATGAAGCA GCAAGTGCTT GGCCATCAAA TTCAAAGACA TGAAAAAGAG
    ACCAATTTAA ACTATGATGG CGTGCCAGCA TATGCAGAGA AACCACTGAA TAGAGAATAT
    GACCAGTCTT ATTGCTATGA TTCTACTTCT GATTATGTAG ATCAGTTTCC CCACAATTAT
    GATTCTCGAA TAAATTTTGA TGATCACGTG CCTACATATG ATGAACAGTG GGGTTATTAT
    GATGACAAAC AGTCATATCC CCAGAGACCA CCATTTGACA GTAAACGCAA TAGGGACTTC
    AATATTAGAC AGCACTCTGA TGAAAACCTT GAACGTAATT ACTTTCAGCA GCCTCGATTG
    GAGGAGCCAC CTCCTGTTGC TTATGACAAT CGCCCCCGCC CATCCCAAAA CTACAAACCA
    ACCCAAGTAC ATTATGAAGA CTCCACAACA GCAGAGTATG ATATGCATAT GCGTTACAAA
    CCTGAACCTC AAACCATTCC TTCTGTGCCA ACATCAAAGT CTTCTGATGC AAAGCCGTAT
    TTTGATCAAC ACATAAGGGG TTATGAACAT AGTCCTACCC AGGTATTTAA CACAAAACCA
    GGGCAGTATG ATACATCTCC TAGAATGGAG GCAATGCCAC CTCCACCTGT TCAGAGTAAG
    CCAGAGCTCC TTTCAACAAG CTCAAAACCT TTGCCTGCCC CTGCTCCTTC CGTTGCTCCT
    GCAGCAACTG AAGAAGACGA TGATCCAGCT ATGAAGCCAC AGTCTGTACT TACTAGAGTG
    AAAATGTTTG AGAACAAACG TTCTAATCAA GGTGAAAAGA ACAAGGATAT AAATGATTCC
    ACTCTTGTTA AGGCCCCAGA TGTTGCACCT AAACCTATAC TTAATCAAGT AAGTGCTACA
    AAGGCTGCCC CCGTTCCACA TTATGATCAG GACAAGGCAA CTTATAGAGT ACCTGAACCT
    CAAAAGCCTC AAGTGAAGCC CCCAGAAGAT ATAATTCGGG CCAACCACTA TGACCCCGAT
    GAAGATGAAG AATACTATCG AAAGCAGCTG TCTTACTTTG ATCGAAGAAG CTTTGAAAAG
    CCCACACCCT TGCTGTCTGG AAACATCCTG CCTGAGCCTG CCAAACCAGC GCAGTCTCAA
    AGCCAACTTA GCTTTAACAG CTACCCCTCC AAGGGAAAGC CTGCAGATTT GGAGCCACTT
    GACAGACCCA TTAATGAAAA ACGCTATGAT CCAGTTCCAC AGGTTACTTC TTCTAACCAG
    TATACTCAGC CTTCACCAGT GGTTGTAAAT CATTCAACAC ACACTAAACA TATGCATTTA
    GAAGCCAGCT CTGTGTCTGT GGAGGTTCCA AGTTCCACAG TGTCTAAGCC AGAGCCCCCA
    CCTACTCAGG CCAAGCCAAC ATTGTTCCGA CCATCAAACA GAGAGGACAC TGTACAGTCA
    AACTTCTATC CTCAGAAGAG TTTCCCTGAA AAAAGCCCTG TAAATGGCAC TGAACAGGCT
    CCAAAAACAG TAACACCATC CTACAATCGT TTTACACCTA AACCTTACAC AAATTCAGCG
    AGACCCTTTG AACGCAAGTT TGAAAGCCCA AAATTTAACC ACAACCTCCT GCCAAATGAA
    GCACAAGTAA AGCCAGAGCT GCCCACTAAA CCTCAGAATT CTCCACAGCC TGTTTTAAAA
    TCAAATACGT CAATTTCTTC TGAACTGGAT AGCATATCCA AGCAAACGGA GAACAAATCT
    AAATATCAAC CAAACAATGT TAATCCAGTG CCTAAAGCAA TCCCAGTCAG TCCTTCTGCA
    TTGGAAGATG ACGATGAAGA TGATGCACAT ACAGTTGTAG CAACAGCTAG AGGCTTCTTT
    AATAGTAATG GAGGTGTGTT AAGTTCCATA GAAACGGGAG TCAGTATCAT CATACCCCAA
    GGAGCAATTC CAGATGGCAT TGAGCAAGAA ATATATTTCA AGGTCTGCAG GGATAACAGT
    ATCCTTCCAC CCTTGGACAA AGAGAAAGGA GAGACGCTAC TTAGTCCATT AGTAATGTGC
    GGACCTCATG GGTTAAAATT CGTGAAACCA GTGGAGCTAC GCTTACCACA CTGTGCGTCT
    ATGACTCCTG ATGGTTGGTC TTTTGCTCTA AAATCATCCG ACACCTCGTC GGGTGACCCA
    AAACACTGGC AGAACAAGTC ACTTCCTGGC GACCCCAACT ATCTTGTCGG AGCAAACTGT
    GTCTCAGTTT TGATAGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755023.1
    CDS25..5451
    Translation

    Target ORF information:

    RefSeq Version XM_031899163.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGGTGGCCT TTTGAGATCA
    CCAAGCACTG AGGCAGCTCA TATTATACTA AGAGACCAAG AACCTTCATC TTTACCAGTG
    CAAATTGACT CAGCAAAGGT TTATAGAAAA GAGGCCTATG CTATGGAAGA GAATCCTAGA
    CAAAATTATT TGATGAAGCA GCAAGTGCTT GGCCATCAAA TTCAAAGACA TGAAAAAGAG
    ACCAATTTAA ACTATGATGG CGTGCCAGCA TATGCAGAGA AACCACTGAA TAGAGAATAT
    GACCAGTCTT ATTGCTATGA TTCTACTTCT GATTATGTAG ATCAGTTTCC CCACAATTAT
    GATTCTCGAA TAAATTTTGA TGATCACGTG CCTACATATG ATGAACAGTG GGGTTATTAT
    GATGACAAAC AGTCATATCC CCAGAGACCA CCATTTGACA GTAAACGCAA TAGGGACTTC
    AATATTAGAC AGCACTCTGA TGAAAACCTT GAACGTAATT ACTTTCAGCA GCCTCGATTG
    GAGGAGCCAC CTCCTGTTGC TTATGACAAT CGCCCCCGCC CATCCCAAAA CTACAAACCA
    ACCCAAGTAC ATTATGAAGA CTCCACAACA GCAGAGTATG ATATGCATAT GCGTTACAAA
    CCTGAACCTC AAACCATTCC TTCTGTGCCA ACATCAAAGT CTTCTGATGC AAAGCCGTAT
    TTTGATCAAC ACATAAGGGG TTATGAACAT AGTCCTACCC AGGTATTTAA CACAAAACCA
    GGGCAGTATG ATACATCTCC TAGAATGGAG GCAATGCCAC CTCCACCTGT TCAGAGTAAG
    CCAGAGCTCC TTTCAACAAG CTCAAAACCT TTGCCTGCCC CTGCTCCTTC CGTTGCTCCT
    GCAGCAACTG AAGAAGACGA TGATCCAGCT ATGAAGCCAC AGTCTGTACT TACTAGAGTG
    AAAATGTTTG AGAACAAACG TTCTAATCAA GGTGAAAAGA ACAAGGATAT AAATGATTCC
    ACTCTTGTTA AGGCCCCAGA TGTTGCACCT AAACCTATAC TTAATCAAGT AAGTGCTACA
    AAGGCTGCCC CCGTTCCACA TTATGATCAG GACAAGGCAA CTTATAGAGT ACCTGAACCT
    CAAAAGCCTC AAGTGAAGCC CCCAGAAGAT ATAATTCGGG CCAACCACTA TGACCCCGAT
    GAAGATGAAG AATACTATCG AAAGCAGCTG TCTTACTTTG ATCGAAGAAG CTTTGAAAAG
    CCCACACCCT TGCTGTCTGG AAACATCCTG CCTGAGCCTG CCAAACCAGC GCAGTCTCAA
    AGCCAACTTA GCTTTAACAG CTACCCCTCC AAGGGAAAGC CTGCAGATTT GGAGCCACTT
    GACAGACCCA TTAATGAAAA ACGCTATGAT CCAGTTCCAC AGGTTACTTC TTCTAACCAG
    TATACTCAGC CTTCACCAGT GGTTGTAAAT CATTCAACAC ACACTAAACA TATGCATTTA
    GAAGCCAGCT CTGTGTCTGT GGAGGTTCCA AGTTCCACAG TGTCTAAGCC AGAGCCCCCA
    CCTACTCAGG CCAAGCCAAC ATTGTTCCGA CCATCAAACA GAGAGGACAC TGTACAGTCA
    AACTTCTATC CTCAGAAGAG TTTCCCTGAA AAAAGCCCTG TAAATGGCAC TGAACAGGCT
    CCAAAAACAG TAACACCATC CTACAATCGT TTTACACCTA AACCTTACAC AAATTCAGCG
    AGACCCTTTG AACGCAAGTT TGAAAGCCCA AAATTTAACC ACAACCTCCT GCCAAATGAA
    GCACAAGTAA AGCCAGAGCT GCCCACTAAA CCTCAGAATT CTCCACAGCC TGTTTTAAAA
    TCAAATACGT CAATTTCTTC TGAACTGGAT AGCATATCCA AGCAAACGGA GAACAAATCT
    AAATATCAAC CAAACAATGT TAATCCAGTG CCTAAAGCAA TCCCAGTCAG TCCTTCTGCA
    TTGGAAGATG ACGATGAAGA TGATGCACAT ACAGTTGTAG CAACAGCTAG AGGCTTCTTT
    AATAGTAATG GAGGTGTGTT AAGTTCCATA GAAACGGGAG TCAGTATCAT CATACCCCAA
    GGAGCAATTC CAGATGGCAT TGAGCAAGAA ATATATTTCA AGGTCTGCAG GGATAACAGT
    ATCCTTCCAC CCTTGGACAA AGAGAAAGGA GAGACGCTAC TTAGTCCATT AGTAATGTGC
    GGACCTCATG GGTTAAAATT CGTGAAACCA GTGGAGCTAC GCTTACCACA CTGTGCGTCT
    ATGACTCCTG ATGGTTGGTC TTTTGCTCTA AAATCATCCG ACACCTCGTC GGGTGACCCA
    AAACACTGGC AGAACAAGTC ACTTCCTGGC GACCCCAACT ATCTTGTCGG AGCAAACTGT
    GTCTCAGTTT TGATAGACCA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55056
    Clone ID Related Accession (Same CDS sequence) XM_031899164.1
    Accession Version XM_031899164.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGGTGG CCTTTTGAGA TCACCAAGCA CTGAGGCAGC TCATATTATA
    CTAAGAGACC AAGAACCTTC ATCTTTACCA GTGCAAATTG ACTCAGCAAA GGTTTATAGA
    AAAGAGGCCT ATGCTATGGA AGAGAATCCT AGACAAAATT ATTTGATGAA GCAGCAAGTG
    CTTGGCCATC AAATTCAAAG ACATGAAAAA GAGACCAATT TAAACTATGA TGGCGTGCCA
    GCATATGCAG AGAAACCACT GAATAGAGAA TATGACCAGT CTTATTGCTA TGATTCTACT
    TCTGATTATG TAGATCAGTT TCCCCACAAT TATGATTCTC GAATAAATTT TGATGATCAC
    GTGCCTACAT ATGATGAACA GTGGGGTTAT TATGATGACA AACAGTCATA TCCCCAGAGA
    CCACCATTTG ACAGTAAACG CAATAGGGAC TTCAATATTA GACAGCACTC TGATGAAAAC
    CTTGAACGTA ATTACTTTCA GCAGCCTCGA TTGGAGGAGC CACCTCCTGT TGCTTATGAC
    AATCGCCCCC GCCCATCCCA AAACTACAAA CCAACCCAAG TACATTATGA AGACTCCACA
    ACAGCAGAGT ATGATATGCA TATGCGTTAC AAACCTGAAC CTCAAACCAT TCCTTCTGTG
    CCAACATCAA AGTCTTCTGA TGCAAAGCCG TATTTTGATC AACACATAAG GGGTTATGAA
    CATAGTCCTA CCCAGGTATT TAACACAAAA CCAGGGCAGT ATGATACATC TCCTAGAATG
    GAGGCAATGC CACCTCCACC TGTTCAGAGT AAGCCAGAGC TCCTTTCAAC AAGCTCAAAA
    CCTTTGCCTG CCCCTGCTCC TTCCGTTGCT CCTGCAGCAA CTGAAGAAGA CGATGATCCA
    GCTATGAAGC CACAGTCTGT ACTTACTAGA GTGAAAATGT TTGAGAACAA ACGTTCTAAT
    CAAGGTGAAA AGAACAAGGA TATAAATGAT TCCACTCTTG TTAAGGCCCC AGATGTTGCA
    CCTAAACCTA TACTTAATCA AGTAAGTGCT ACAAAGGCTG CCCCCGTTCC ACATTATGAT
    CAGGACAAGG CAACTTATAG AGTACCTGAA CCTCAAAAGC CTCAAGTGAA GCCCCCAGAA
    GATATAATTC GGGCCAACCA CTATGACCCC GATGAAGATG AAGAATACTA TCGAAAGCAG
    CTGTCTTACT TTGATCGAAG AAGCTTTGAA AAGCCCACAC CCTTGCTGTC TGGAAACATC
    CTGCCTGAGC CTGCCAAACC AGCGCAGTCT CAAAGCCAAC TTAGCTTTAA CAGCTACCCC
    TCCAAGGGAA AGCCTGCAGA TTTGGAGCCA CTTGACAGAC CCATTAATGA AAAACGCTAT
    GATCCAGTTC CACAGGTTAC TTCTTCTAAC CAGTATACTC AGCCTTCACC AGTGGTTGTA
    AATCATTCAA CACACACTAA ACATATGCAT TTAGAAGCCA GCTCTGTGTC TGTGGAGGTT
    CCAAGTTCCA CAGTGTCTAA GCCAGAGCCC CCACCTACTC AGGCCAAGCC AACATTGTTC
    CGACCATCAA ACAGAGAGGA CACTGTACAG TCAAACTTCT ATCCTCAGAA GAGTTTCCCT
    GAAAAAAGCC CTGTAAATGG CACTGAACAG GCTCCAAAAA CAGTAACACC ATCCTACAAT
    CGTTTTACAC CTAAACCTTA CACAAATTCA GCGAGACCCT TTGAACGCAA GTTTGAAAGC
    CCAAAATTTA ACCACAACCT CCTGCCAAAT GAAGCACAAG TAAAGCCAGA GCTGCCCACT
    AAACCTCAGA ATTCTCCACA GCCTGTTTTA AAATCAAATA CGTCAATTTC TTCTGAACTG
    GATAGCATAT CCAAGCAAAC GGAGAACAAA TCTAAATATC AACCAAACAA TGTTAATCCA
    GTGCCTAAAG CAATCCCAGT CAGTCCTTCT GCATTGGAAG ATGACGATGA AGATGATGCA
    CATACAGTTG TAGCAACAGC TAGAGGCTTC TTTAATAGTA ATGGAGGTGT GTTAAGTTCC
    ATAGAAACGG GAGTCAGTAT CATCATACCC CAAGGAGCAA TTCCAGATGG CATTGAGCAA
    GAAATATATT TCAAGGTCTG CAGGGATAAC AGTATCCTTC CACCCTTGGA CAAAGAGAAA
    GGAGAGACGC TACTTAGTCC ATTAGTAATG TGCGGACCTC ATGGGTTAAA ATTCGTGAAA
    CCAGTGGAGC TACGCTTACC ACACTGTGCG TCTATGACTC CTGATGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACACCTC GTCGGGTGAC CCAAAACACT GGCAGAACAA GTCACTTCCT
    GGCGACCCCA ACTATCTTGT CGGAGCAAAC TGTGTCTCAG TTTTGATAGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755024.1
    CDS25..5364
    Translation

    Target ORF information:

    RefSeq Version XM_031899164.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGGTGG CCTTTTGAGA TCACCAAGCA CTGAGGCAGC TCATATTATA
    CTAAGAGACC AAGAACCTTC ATCTTTACCA GTGCAAATTG ACTCAGCAAA GGTTTATAGA
    AAAGAGGCCT ATGCTATGGA AGAGAATCCT AGACAAAATT ATTTGATGAA GCAGCAAGTG
    CTTGGCCATC AAATTCAAAG ACATGAAAAA GAGACCAATT TAAACTATGA TGGCGTGCCA
    GCATATGCAG AGAAACCACT GAATAGAGAA TATGACCAGT CTTATTGCTA TGATTCTACT
    TCTGATTATG TAGATCAGTT TCCCCACAAT TATGATTCTC GAATAAATTT TGATGATCAC
    GTGCCTACAT ATGATGAACA GTGGGGTTAT TATGATGACA AACAGTCATA TCCCCAGAGA
    CCACCATTTG ACAGTAAACG CAATAGGGAC TTCAATATTA GACAGCACTC TGATGAAAAC
    CTTGAACGTA ATTACTTTCA GCAGCCTCGA TTGGAGGAGC CACCTCCTGT TGCTTATGAC
    AATCGCCCCC GCCCATCCCA AAACTACAAA CCAACCCAAG TACATTATGA AGACTCCACA
    ACAGCAGAGT ATGATATGCA TATGCGTTAC AAACCTGAAC CTCAAACCAT TCCTTCTGTG
    CCAACATCAA AGTCTTCTGA TGCAAAGCCG TATTTTGATC AACACATAAG GGGTTATGAA
    CATAGTCCTA CCCAGGTATT TAACACAAAA CCAGGGCAGT ATGATACATC TCCTAGAATG
    GAGGCAATGC CACCTCCACC TGTTCAGAGT AAGCCAGAGC TCCTTTCAAC AAGCTCAAAA
    CCTTTGCCTG CCCCTGCTCC TTCCGTTGCT CCTGCAGCAA CTGAAGAAGA CGATGATCCA
    GCTATGAAGC CACAGTCTGT ACTTACTAGA GTGAAAATGT TTGAGAACAA ACGTTCTAAT
    CAAGGTGAAA AGAACAAGGA TATAAATGAT TCCACTCTTG TTAAGGCCCC AGATGTTGCA
    CCTAAACCTA TACTTAATCA AGTAAGTGCT ACAAAGGCTG CCCCCGTTCC ACATTATGAT
    CAGGACAAGG CAACTTATAG AGTACCTGAA CCTCAAAAGC CTCAAGTGAA GCCCCCAGAA
    GATATAATTC GGGCCAACCA CTATGACCCC GATGAAGATG AAGAATACTA TCGAAAGCAG
    CTGTCTTACT TTGATCGAAG AAGCTTTGAA AAGCCCACAC CCTTGCTGTC TGGAAACATC
    CTGCCTGAGC CTGCCAAACC AGCGCAGTCT CAAAGCCAAC TTAGCTTTAA CAGCTACCCC
    TCCAAGGGAA AGCCTGCAGA TTTGGAGCCA CTTGACAGAC CCATTAATGA AAAACGCTAT
    GATCCAGTTC CACAGGTTAC TTCTTCTAAC CAGTATACTC AGCCTTCACC AGTGGTTGTA
    AATCATTCAA CACACACTAA ACATATGCAT TTAGAAGCCA GCTCTGTGTC TGTGGAGGTT
    CCAAGTTCCA CAGTGTCTAA GCCAGAGCCC CCACCTACTC AGGCCAAGCC AACATTGTTC
    CGACCATCAA ACAGAGAGGA CACTGTACAG TCAAACTTCT ATCCTCAGAA GAGTTTCCCT
    GAAAAAAGCC CTGTAAATGG CACTGAACAG GCTCCAAAAA CAGTAACACC ATCCTACAAT
    CGTTTTACAC CTAAACCTTA CACAAATTCA GCGAGACCCT TTGAACGCAA GTTTGAAAGC
    CCAAAATTTA ACCACAACCT CCTGCCAAAT GAAGCACAAG TAAAGCCAGA GCTGCCCACT
    AAACCTCAGA ATTCTCCACA GCCTGTTTTA AAATCAAATA CGTCAATTTC TTCTGAACTG
    GATAGCATAT CCAAGCAAAC GGAGAACAAA TCTAAATATC AACCAAACAA TGTTAATCCA
    GTGCCTAAAG CAATCCCAGT CAGTCCTTCT GCATTGGAAG ATGACGATGA AGATGATGCA
    CATACAGTTG TAGCAACAGC TAGAGGCTTC TTTAATAGTA ATGGAGGTGT GTTAAGTTCC
    ATAGAAACGG GAGTCAGTAT CATCATACCC CAAGGAGCAA TTCCAGATGG CATTGAGCAA
    GAAATATATT TCAAGGTCTG CAGGGATAAC AGTATCCTTC CACCCTTGGA CAAAGAGAAA
    GGAGAGACGC TACTTAGTCC ATTAGTAATG TGCGGACCTC ATGGGTTAAA ATTCGTGAAA
    CCAGTGGAGC TACGCTTACC ACACTGTGCG TCTATGACTC CTGATGGTTG GTCTTTTGCT
    CTAAAATCAT CCGACACCTC GTCGGGTGAC CCAAAACACT GGCAGAACAA GTCACTTCCT
    GGCGACCCCA ACTATCTTGT CGGAGCAAAC TGTGTCTCAG TTTTGATAGA CCACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55057
    Clone ID Related Accession (Same CDS sequence) XM_031899165.1
    Accession Version XM_031899165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTTTATAG AAAAGAGGCC
    TATGCTATGG AAGAGAATCC TAGACAAAAT TATTTGATGA AGCAGCAAGT GCTTGGCCAT
    CAAATTCAAA GACATGAAAA AGAGACCAAT TTAAACTATG ATGGCGTGCC AGCATATGCA
    GAGAAACCAC TGAATAGAGA ATATGACCAG TCTTATTGCT ATGATTCTAC TTCTGATTAT
    GTAGATCAGT TTCCCCACAA TTATGATTCT CGAATAAATT TTGATGATCA CGTGCCTACA
    TATGATGAAC AGTGGGGTTA TTATGATGAC AAACAGTCAT ATCCCCAGAG ACCACCATTT
    GACAGTAAAC GCAATAGGGA CTTCAATATT AGACAGCACT CTGATGAAAA CCTTGAACGT
    AATTACTTTC AGCAGCCTCG ATTGGAGGAG CCACCTCCTG TTGCTTATGA CAATCGCCCC
    CGCCCATCCC AAAACTACAA ACCAACCCAA GTACATTATG AAGACTCCAC AACAGCAGAG
    TATGATATGC ATATGCGTTA CAAACCTGAA CCTCAAACCA TTCCTTCTGT GCCAACATCA
    AAGTCTTCTG ATGCAAAGCC GTATTTTGAT CAACACATAA GGGGTTATGA ACATAGTCCT
    ACCCAGGTAT TTAACACAAA ACCAGGGCAG TATGATACAT CTCCTAGAAT GGAGGCAATG
    CCACCTCCAC CTGTTCAGAG TAAGCCAGAG CTCCTTTCAA CAAGCTCAAA ACCTTTGCCT
    GCCCCTGCTC CTTCCGTTGC TCCTGCAGCA ACTGAAGAAG ACGATGATCC AGCTATGAAG
    CCACAGTCTG TACTTACTAG AGTGAAAATG TTTGAGAACA AACGTTCTAA TCAAGGTGAA
    AAGAACAAGG ATATAAATGA TTCCACTCTT GTTAAGGCCC CAGATGTTGC ACCTAAACCT
    ATACTTAATC AAGTAAGTGC TACAAAGGCT GCCCCCGTTC CACATTATGA TCAGGACAAG
    GCAACTTATA GAGTACCTGA ACCTCAAAAG CCTCAAGTGA AGCCCCCAGA AGATATAATT
    CGGGCCAACC ACTATGACCC CGATGAAGAT GAAGAATACT ATCGAAAGCA GCTGTCTTAC
    TTTGATCGAA GAAGCTTTGA AAAGCCCACA CCCTTGCTGT CTGGAAACAT CCTGCCTGAG
    CCTGCCAAAC CAGCGCAGTC TCAAAGCCAA CTTAGCTTTA ACAGCTACCC CTCCAAGGGA
    AAGCCTGCAG ATTTGGAGCC ACTTGACAGA CCCATTAATG AAAAACGCTA TGATCCAGTT
    CCACAGGTTA CTTCTTCTAA CCAGTATACT CAGCCTTCAC CAGTGGTTGT AAATCATTCA
    ACACACACTA AACATATGCA TTTAGAAGCC AGCTCTGTGT CTGTGGAGGT TCCAAGTTCC
    ACAGTGTCTA AGCCAGAGCC CCCACCTACT CAGGCCAAGC CAACATTGTT CCGACCATCA
    AACAGAGAGG ACACTGTACA GTCAAACTTC TATCCTCAGA AGAGTTTCCC TGAAAAAAGC
    CCTGTAAATG GCACTGAACA GGCTCCAAAA ACAGTAACAC CATCCTACAA TCGTTTTACA
    CCTAAACCTT ACACAAATTC AGCGAGACCC TTTGAACGCA AGTTTGAAAG CCCAAAATTT
    AACCACAACC TCCTGCCAAA TGAAGCACAA GTAAAGCCAG AGCTGCCCAC TAAACCTCAG
    AATTCTCCAC AGCCTGTTTT AAAATCAAAT ACGTCAATTT CTTCTGAACT GGATAGCATA
    TCCAAGCAAA CGGAGAACAA ATCTAAATAT CAACCAAACA ATGTTAATCC AGTGCCTAAA
    GCAATCCCAG TCAGTCCTTC TGCATTGGAA GATGACGATG AAGATGATGC ACATACAGTT
    GTAGCAACAG CTAGAGGCTT CTTTAATAGT AATGGAGGTG TGTTAAGTTC CATAGAAACG
    GGAGTCAGTA TCATCATACC CCAAGGAGCA ATTCCAGATG GCATTGAGCA AGAAATATAT
    TTCAAGGTCT GCAGGGATAA CAGTATCCTT CCACCCTTGG ACAAAGAGAA AGGAGAGACG
    CTACTTAGTC CATTAGTAAT GTGCGGACCT CATGGGTTAA AATTCGTGAA ACCAGTGGAG
    CTACGCTTAC CACACTGTGC GTCTATGACT CCTGATGGTT GGTCTTTTGC TCTAAAATCA
    TCCGACACCT CGTCGGGTGA CCCAAAACAC TGGCAGAACA AGTCACTTCC TGGCGACCCC
    AACTATCTTG TCGGAGCAAA CTGTGTCTCA GTTTTGATAG ACCACTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755025.1
    CDS25..5355
    Translation

    Target ORF information:

    RefSeq Version XM_031899165.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGAAAGC AGAAGCCTTA CCTGCAGCCC CTTACCTTTC TGTGTCGTCT
    GAAACAAACC ATACAACTTC AACTGCTGCT GTTAATCCCA TGGTTTATAG AAAAGAGGCC
    TATGCTATGG AAGAGAATCC TAGACAAAAT TATTTGATGA AGCAGCAAGT GCTTGGCCAT
    CAAATTCAAA GACATGAAAA AGAGACCAAT TTAAACTATG ATGGCGTGCC AGCATATGCA
    GAGAAACCAC TGAATAGAGA ATATGACCAG TCTTATTGCT ATGATTCTAC TTCTGATTAT
    GTAGATCAGT TTCCCCACAA TTATGATTCT CGAATAAATT TTGATGATCA CGTGCCTACA
    TATGATGAAC AGTGGGGTTA TTATGATGAC AAACAGTCAT ATCCCCAGAG ACCACCATTT
    GACAGTAAAC GCAATAGGGA CTTCAATATT AGACAGCACT CTGATGAAAA CCTTGAACGT
    AATTACTTTC AGCAGCCTCG ATTGGAGGAG CCACCTCCTG TTGCTTATGA CAATCGCCCC
    CGCCCATCCC AAAACTACAA ACCAACCCAA GTACATTATG AAGACTCCAC AACAGCAGAG
    TATGATATGC ATATGCGTTA CAAACCTGAA CCTCAAACCA TTCCTTCTGT GCCAACATCA
    AAGTCTTCTG ATGCAAAGCC GTATTTTGAT CAACACATAA GGGGTTATGA ACATAGTCCT
    ACCCAGGTAT TTAACACAAA ACCAGGGCAG TATGATACAT CTCCTAGAAT GGAGGCAATG
    CCACCTCCAC CTGTTCAGAG TAAGCCAGAG CTCCTTTCAA CAAGCTCAAA ACCTTTGCCT
    GCCCCTGCTC CTTCCGTTGC TCCTGCAGCA ACTGAAGAAG ACGATGATCC AGCTATGAAG
    CCACAGTCTG TACTTACTAG AGTGAAAATG TTTGAGAACA AACGTTCTAA TCAAGGTGAA
    AAGAACAAGG ATATAAATGA TTCCACTCTT GTTAAGGCCC CAGATGTTGC ACCTAAACCT
    ATACTTAATC AAGTAAGTGC TACAAAGGCT GCCCCCGTTC CACATTATGA TCAGGACAAG
    GCAACTTATA GAGTACCTGA ACCTCAAAAG CCTCAAGTGA AGCCCCCAGA AGATATAATT
    CGGGCCAACC ACTATGACCC CGATGAAGAT GAAGAATACT ATCGAAAGCA GCTGTCTTAC
    TTTGATCGAA GAAGCTTTGA AAAGCCCACA CCCTTGCTGT CTGGAAACAT CCTGCCTGAG
    CCTGCCAAAC CAGCGCAGTC TCAAAGCCAA CTTAGCTTTA ACAGCTACCC CTCCAAGGGA
    AAGCCTGCAG ATTTGGAGCC ACTTGACAGA CCCATTAATG AAAAACGCTA TGATCCAGTT
    CCACAGGTTA CTTCTTCTAA CCAGTATACT CAGCCTTCAC CAGTGGTTGT AAATCATTCA
    ACACACACTA AACATATGCA TTTAGAAGCC AGCTCTGTGT CTGTGGAGGT TCCAAGTTCC
    ACAGTGTCTA AGCCAGAGCC CCCACCTACT CAGGCCAAGC CAACATTGTT CCGACCATCA
    AACAGAGAGG ACACTGTACA GTCAAACTTC TATCCTCAGA AGAGTTTCCC TGAAAAAAGC
    CCTGTAAATG GCACTGAACA GGCTCCAAAA ACAGTAACAC CATCCTACAA TCGTTTTACA
    CCTAAACCTT ACACAAATTC AGCGAGACCC TTTGAACGCA AGTTTGAAAG CCCAAAATTT
    AACCACAACC TCCTGCCAAA TGAAGCACAA GTAAAGCCAG AGCTGCCCAC TAAACCTCAG
    AATTCTCCAC AGCCTGTTTT AAAATCAAAT ACGTCAATTT CTTCTGAACT GGATAGCATA
    TCCAAGCAAA CGGAGAACAA ATCTAAATAT CAACCAAACA ATGTTAATCC AGTGCCTAAA
    GCAATCCCAG TCAGTCCTTC TGCATTGGAA GATGACGATG AAGATGATGC ACATACAGTT
    GTAGCAACAG CTAGAGGCTT CTTTAATAGT AATGGAGGTG TGTTAAGTTC CATAGAAACG
    GGAGTCAGTA TCATCATACC CCAAGGAGCA ATTCCAGATG GCATTGAGCA AGAAATATAT
    TTCAAGGTCT GCAGGGATAA CAGTATCCTT CCACCCTTGG ACAAAGAGAA AGGAGAGACG
    CTACTTAGTC CATTAGTAAT GTGCGGACCT CATGGGTTAA AATTCGTGAA ACCAGTGGAG
    CTACGCTTAC CACACTGTGC GTCTATGACT CCTGATGGTT GGTCTTTTGC TCTAAAATCA
    TCCGACACCT CGTCGGGTGA CCCAAAACAC TGGCAGAACA AGTCACTTCC TGGCGACCCC
    AACTATCTTG TCGGAGCAAA CTGTGTCTCA GTTTTGATAG ACCACTTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55058
    Clone ID Related Accession (Same CDS sequence) XM_031899166.1
    Accession Version XM_031899166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCTA GAGCGGCTGC CAATAAGAAT ACTGTAATGG AAGAAACTGC AATATGGGAA 
    CAGCAAACAG TTACTCTCCA CAGGGCCCCT GGATTTGGAT TTGGCATTGC AATATCTGGA
    GGCAGAGATA ACCCACATTT TCAAAGTGGG GAAACATCAA TTGTCATCTC TGATGTTCTT
    AAAGGAGGGC CTGCAGAAGG TTTGCTTCAG GAAAATGATC GTGTTGCAAT GGTCAATGGG
    GTTTCTATGG ATAATGTCGA GCATGCCTTT GCTGTTCAGC AGTTGAGGAA AAGTGGAAAA
    AATGCCAAAA TTACTATCCG GCGGAAGAAA AAAGTGCAAC TTCCAGTAGT TCGTTCTGAC
    CCAGACCCAG TGTCTGAGAA TGAAGAGGAC AGTTATGAGG AGGATGTGCC TGACCAGAGA
    AGTGCCCACA GTGGTCCTGG TGCCAGCAGG AGAAGTGAAA AAAGTTGGGT GAGGGACAGA
    AGTGCAAGCA GAGAAAGAAG TTTATCACCA AGATCAGACA GAAGATCTGT TTCTTCCAGT
    CAACCTGCTA AACCTACCAA AGTGACCTTG GTGAAATCTA GAAAAAATGA AGAATATGGT
    TTAAGGTTGG CTAGCCACAT ATTTGTAAAA GAAATTTCCC AAGACAGTCT TGCGGCAAGA
    GATGGAAATA TTCAAGAAGG GGATGTTGTG CTTAAGATTA ATGGCACAGT CACAGAAAAC
    ATGTCTTTGG CTGATGCAAA AACATTAATA GAAAGGTCGA AGGGCAAATT AAAAATGGTA
    GTTCAAAGAG ATGAACGAGC AACTCTACTT AATGTCCCAG ATCTAGATGA CAGTCTCCAT
    TCTGCAAATG CTTCAGAAAG AGATGACATT TCAGAAATTC AGTCGCTAAC ATCAGATCAT
    TCCAACCGTT CACATGATAA ACCTCGCCGT AGCCGTTCAC GGTCACCGGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAACAGCACA GCAACAGCAG TAGTCGAAGT
    CGAGAGGATG AAAGAGTTTC AAAGCCTGGT GCAATATCTA CTCCGGTGAA GAATGTCAAT
    AGTGTACCTC TTTCCAGAAC TGCAGAAGAA CCCTTTTTAG AAAAGGCAGA TAAACAAACA
    CCTCCTCTTC CAGAGCCAAA ACCTGTATAT GCCCAGCCAG GACAACCAGA TGTGGATTTG
    CCAGTCAGCC CATCTGATGG ACCTTTCCCT AATTCTACAC ATGAAGATGG CATGCTTCGG
    CCAAGCATGA AACTGGTGAA GTTCAGGAAA GGAGACAGTG TAGGCCTTCG TTTGGCTGGT
    GGTAATGATG TGGGAATTTT TGTTGCTGGT GTATTAGACG ACAGTCCTGC CGCAAAAGAA
    GGTTTGGAGG AAGGGGACCA GATACTCAGA GTTAACAATG TGGATTTCAC CAATATAATA
    AGGGAAGAAG CGGTACTGTT CCTTCTTGAT CTGCCCAAAG GAGAAGAGGT TACCATTCTT
    GCTCAGAAAA AGAAAGATGT GTATCGCCGC ATTGTTGAGT CAGATGTTGG GGATTCATTT
    TATATAAGAA CTCACTTTGA ATATGAGAAA GAGTCACCAT ACGGGCTAAG TTTCAACAAA
    GGAGAGGTCT TTCGAGTTGT GGATACTCTT TACAATGGCA AACTAGGGTC ATGGTTGGCC
    ATTAGAATTG GTAGAAATCA CAAGGAGGTG GAAAGAGGGA TAATCCCCAA CAAGAATAGG
    GCTGAGCAAC TGGCTAGTGT ACAGTACACT CTTCCTAAAA CTGCTGGTGG AGATCGTGCA
    GACTTCTGGA GATTCCGTGG CCTTCGTAGT TCCAAGCGAA ATCTACGGAA AAGCAGAGAG
    GATCTTTCTG CGCAACCTGT TCAAACAAAG TTCCCTGCTT ATGAGAGAGT CGTCTTGAGA
    GAAGCTGGAT TTCTCAGGCC TGTGGTTATT TTTGGCCCTA TTGCTGACGT TGCAAGAGAA
    AAGTTGGCAA GAGAAGAACC AGATATTTAT GAAGTAGCAA GAAGTGAACC CAGAGATGCA
    GGTACTGACC AGCGAAGTTC TGGAATTATC CGCCTTCATA CTATTAAACA GATTATTGAT
    AGGGATAAGC ATGCTCTTCT AGATGTGACT CCAAATGCTG TGGACCGCTT AAATTATGCA
    CAATGGTATC CGATAGTAGT GTTTCTTAAC CCAGACTCTA AACAAGGTGT GAAAACAATG
    AGAATGAGGT TATGCCCAGA ATCACGCAAA AGTGCCAGGA AACTATATGA ACGTTCACAC
    AAACTACGCA AAAACAATCA CCATCTTTTC ACAACTACAA TAAATCTCAA CTCCATGAAT
    GATGGCTGGT ATGGTGGCCT TAAAGAGGCA GCACAGCAGC AACAGAACCA GCTAGTTTGG
    GTCTCAGAGG GCAAAGCAGA AGGTGGCACA GATGATGACC TTGATTTGCA TGACGACCGT
    CTTTCCTACC TATCAGCACC AGGTAGTGAA TATTCAATGT ATAGCACTGA TAGTAGGCAC
    ACATCAGACT ATGAAGACAC AGACACAGAA GGAGGGGCTT ACACTGACCA GGACCTGGAT
    GAGACACTTA ATGATGAGGC TGGGACTCCA CCAGAATCTG CAATCACACG ATCTTCTGAA
    CCAGTCAGAG AAGACACATC AGGGATGCAA GAAAACCCAG CATACCCAAC ATATCCACCT
    CAGCCAATCC TTAGAACAGA TTCTCCTGGG TATAAAAATG TACCTTCTCA ACAGAAAGCA
    GAAGCCTTAC CTGCAGCCCC TTACCTTTCT GTGTCGTCTG AAACAAACCA TACAACTTCA
    ACTGCTGCTG TTAATCCCAT GGTAAATCTA AATAATGTTC GATTGGAGGA TCCCAACCAT
    GCCCCTTACA GCTCTTATCA ATCACAGGGT GGCCTTTTGA GATCACCAAG CACTGAGGCA
    GCTCATATTA TACTAAGAGA CCAAGAACCT TCATCTTTAC CAGTGCAAAT TGACTCAGCA
    AAGGTTTATA GAAAAGAGGC CTATGCTATG GAAGAGAATC CTAGACAAAA TTATTTGATG
    AAGCAGCAAG TGCTTGGCCA TCAAATTCAA AGACATGAAA AAGAGACCAA TTTAAACTAT
    GATGGCGTGC CAGCATATGC AGAGAAACCA CTGAATAGAG AATATGACCA GTCTTATTGC
    TATGATTCTA CTTCTGATTA TGTAGATCAG TTTCCCCACA ATTATGATTC TCGAATAAAT
    TTTGATGATC ACGTGCCTAC ATATGATGAA CAGTGGGGTT ATTATGATGA CAAACAGTCA
    TATCCCCAGA GACCACCATT TGACAGTAAA CGCAATAGGG ACTTCAATAT TAGACAGCAC
    TCTGATGAAA ACCTTGAACG TAATTACTTT CAGCAGCCTC GATTGGAGGA GCCACCTCCT
    GTTGCTTATG ACAATCGCCC CCGCCCATCC CAAAACTACA AACCAACCCA AGTACATTAT
    GAAGACTCCA CAACAGCAGA GTATGATATG CATATGCGTT ACAAACCTGA ACCTCAAACC
    ATTCCTTCTG TGCCAACATC AAAGTCTTCT GATGCAAAGC CGTATTTTGA TCAACACATA
    AGGGGTTATG AACATAGTCC TACCCAGGTA TTTAACACAA AACCAGGGCA GTATGATACA
    TCTCCTAGAA TGGAGGCAAT GCCACCTCCA CCTGTTCAGA GTAAGCCAGA GCTCCTTTCA
    ACAAGCTCAA AACCTTTGCC TGCCCCTGCT CCTTCCGTTG CTCCTGCAGC AACTGAAGAA
    GACGATGATC CAGCTATGAA GCCACAGTCT GTACTTACTA GAGTGAAAAT GTTTGAGAAC
    AAACGTTCTA ATCAAGGTGA AAAGAACAAG GATATAAATG ATTCCACTCT TGTTAAGGCC
    CCAGATGTTG CACCTAAACC TATACTTAAT CAAGTAAGTG CTACAAAGGC TGCCCCCGTT
    CCACATTATG ATCAGGACAA GGCAACTTAT AGAGTACCTG AACCTCAAAA GCCTCAAGTG
    AAGCCCCCAG AAGATATAAT TCGGGCCAAC CACTATGACC CCGATGAAGA TGAAGAATAC
    TATCGAAAGC AGCTGTCTTA CTTTGATCGA AGAAGCTTTG AAAAGCCCAC ACCCTTGCTG
    TCTGGAAACA TCCTGCCTGA GCCTGCCAAA CCAGCGCAGT CTCAAAGCCA ACTTAGCTTT
    AACAGCTACC CCTCCAAGGG AAAGCCTGCA GATTTGGAGC CACTTGACAG ACCCATTAAT
    GAAAAACGCT ATGATCCAGT TCCACAGGTT ACTTCTTCTA ACCAGTATAC TCAGCCTTCA
    CCAGTGGTTG TAAATCATTC AACACACACT AAACATATGC ATTTAGAAGC CAGCTCTGTG
    TCTGTGGAGG TTCCAAGTTC CACAGTGTCT AAGCCAGAGC CCCCACCTAC TCAGGCCAAG
    CCAACATTGT TCCGACCATC AAACAGAGAG GACACTGTAC AGTCAAACTT CTATCCTCAG
    AAGAGTTTCC CTGAAAAAAG CCCTGTAAAT GGCACTGAAC AGGCTCCAAA AACAGTAACA
    CCATCCTACA ATCGTTTTAC ACCTAAACCT TACACAAATT CAGCGAGACC CTTTGAACGC
    AAGTTTGAAA GCCCAAAATT TAACCACAAC CTCCTGCCAA ATGAAGCACA AGTAAAGCCA
    GAGCTGCCCA CTAAACCTCA GAATTCTCCA CAGCCTGTTT TAAAATCAAA TACGTCAATT
    TCTTCTGAAC TGGATAGCAT ATCCAAGCAA ACGGAGAACA AATCTAAATA TCAACCAAAC
    AATGTTAATC CAGTGCCTAA AGCAATCCCA GTCAGTCCTT CTGCATTGGA AGATGACGAT
    GAAGATGATG CACATACAGT TGTAGCAACA GCTAGAGGCT TCTTTAATAG TAATGGAGGT
    GTGTTAAGTT CCATAGAAAC GGGAGTCAGT ATCATCATAC CCCAAGGAGC AATTCCAGAT
    GGCATTGAGC AAGAAATATA TTTCAAGGTC TGCAGGGATA ACAGTATCCT TCCACCCTTG
    GACAAAGAGA AAGGAGAGAC GCTACTTAGT CCATTAGTAA TGTGCGGACC TCATGGGTTA
    AAATTCGTGA AACCAGTGGA GCTACGCTTA CCACACTGTG CGTCTATGAC TCCTGATGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACACC TCGTCGGGTG ACCCAAAACA CTGGCAGAAC
    AAGTCACTTC CTGGCGACCC CAACTATCTT GTCGGAGCAA ACTGTGTCTC AGTTTTGATA
    GACCACTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755026.1
    CDS1576..6867
    Translation

    Target ORF information:

    RefSeq Version XM_031899166.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGTCTA GAGCGGCTGC CAATAAGAAT ACTGTAATGG AAGAAACTGC AATATGGGAA 
    CAGCAAACAG TTACTCTCCA CAGGGCCCCT GGATTTGGAT TTGGCATTGC AATATCTGGA
    GGCAGAGATA ACCCACATTT TCAAAGTGGG GAAACATCAA TTGTCATCTC TGATGTTCTT
    AAAGGAGGGC CTGCAGAAGG TTTGCTTCAG GAAAATGATC GTGTTGCAAT GGTCAATGGG
    GTTTCTATGG ATAATGTCGA GCATGCCTTT GCTGTTCAGC AGTTGAGGAA AAGTGGAAAA
    AATGCCAAAA TTACTATCCG GCGGAAGAAA AAAGTGCAAC TTCCAGTAGT TCGTTCTGAC
    CCAGACCCAG TGTCTGAGAA TGAAGAGGAC AGTTATGAGG AGGATGTGCC TGACCAGAGA
    AGTGCCCACA GTGGTCCTGG TGCCAGCAGG AGAAGTGAAA AAAGTTGGGT GAGGGACAGA
    AGTGCAAGCA GAGAAAGAAG TTTATCACCA AGATCAGACA GAAGATCTGT TTCTTCCAGT
    CAACCTGCTA AACCTACCAA AGTGACCTTG GTGAAATCTA GAAAAAATGA AGAATATGGT
    TTAAGGTTGG CTAGCCACAT ATTTGTAAAA GAAATTTCCC AAGACAGTCT TGCGGCAAGA
    GATGGAAATA TTCAAGAAGG GGATGTTGTG CTTAAGATTA ATGGCACAGT CACAGAAAAC
    ATGTCTTTGG CTGATGCAAA AACATTAATA GAAAGGTCGA AGGGCAAATT AAAAATGGTA
    GTTCAAAGAG ATGAACGAGC AACTCTACTT AATGTCCCAG ATCTAGATGA CAGTCTCCAT
    TCTGCAAATG CTTCAGAAAG AGATGACATT TCAGAAATTC AGTCGCTAAC ATCAGATCAT
    TCCAACCGTT CACATGATAA ACCTCGCCGT AGCCGTTCAC GGTCACCGGA CCAGAGGTCA
    GAGCCTTCAG ACCATTCCAG ACATTCTCCA CAACAGCACA GCAACAGCAG TAGTCGAAGT
    CGAGAGGATG AAAGAGTTTC AAAGCCTGGT GCAATATCTA CTCCGGTGAA GAATGTCAAT
    AGTGTACCTC TTTCCAGAAC TGCAGAAGAA CCCTTTTTAG AAAAGGCAGA TAAACAAACA
    CCTCCTCTTC CAGAGCCAAA ACCTGTATAT GCCCAGCCAG GACAACCAGA TGTGGATTTG
    CCAGTCAGCC CATCTGATGG ACCTTTCCCT AATTCTACAC ATGAAGATGG CATGCTTCGG
    CCAAGCATGA AACTGGTGAA GTTCAGGAAA GGAGACAGTG TAGGCCTTCG TTTGGCTGGT
    GGTAATGATG TGGGAATTTT TGTTGCTGGT GTATTAGACG ACAGTCCTGC CGCAAAAGAA
    GGTTTGGAGG AAGGGGACCA GATACTCAGA GTTAACAATG TGGATTTCAC CAATATAATA
    AGGGAAGAAG CGGTACTGTT CCTTCTTGAT CTGCCCAAAG GAGAAGAGGT TACCATTCTT
    GCTCAGAAAA AGAAAGATGT GTATCGCCGC ATTGTTGAGT CAGATGTTGG GGATTCATTT
    TATATAAGAA CTCACTTTGA ATATGAGAAA GAGTCACCAT ACGGGCTAAG TTTCAACAAA
    GGAGAGGTCT TTCGAGTTGT GGATACTCTT TACAATGGCA AACTAGGGTC ATGGTTGGCC
    ATTAGAATTG GTAGAAATCA CAAGGAGGTG GAAAGAGGGA TAATCCCCAA CAAGAATAGG
    GCTGAGCAAC TGGCTAGTGT ACAGTACACT CTTCCTAAAA CTGCTGGTGG AGATCGTGCA
    GACTTCTGGA GATTCCGTGG CCTTCGTAGT TCCAAGCGAA ATCTACGGAA AAGCAGAGAG
    GATCTTTCTG CGCAACCTGT TCAAACAAAG TTCCCTGCTT ATGAGAGAGT CGTCTTGAGA
    GAAGCTGGAT TTCTCAGGCC TGTGGTTATT TTTGGCCCTA TTGCTGACGT TGCAAGAGAA
    AAGTTGGCAA GAGAAGAACC AGATATTTAT GAAGTAGCAA GAAGTGAACC CAGAGATGCA
    GGTACTGACC AGCGAAGTTC TGGAATTATC CGCCTTCATA CTATTAAACA GATTATTGAT
    AGGGATAAGC ATGCTCTTCT AGATGTGACT CCAAATGCTG TGGACCGCTT AAATTATGCA
    CAATGGTATC CGATAGTAGT GTTTCTTAAC CCAGACTCTA AACAAGGTGT GAAAACAATG
    AGAATGAGGT TATGCCCAGA ATCACGCAAA AGTGCCAGGA AACTATATGA ACGTTCACAC
    AAACTACGCA AAAACAATCA CCATCTTTTC ACAACTACAA TAAATCTCAA CTCCATGAAT
    GATGGCTGGT ATGGTGGCCT TAAAGAGGCA GCACAGCAGC AACAGAACCA GCTAGTTTGG
    GTCTCAGAGG GCAAAGCAGA AGGTGGCACA GATGATGACC TTGATTTGCA TGACGACCGT
    CTTTCCTACC TATCAGCACC AGGTAGTGAA TATTCAATGT ATAGCACTGA TAGTAGGCAC
    ACATCAGACT ATGAAGACAC AGACACAGAA GGAGGGGCTT ACACTGACCA GGACCTGGAT
    GAGACACTTA ATGATGAGGC TGGGACTCCA CCAGAATCTG CAATCACACG ATCTTCTGAA
    CCAGTCAGAG AAGACACATC AGGGATGCAA GAAAACCCAG CATACCCAAC ATATCCACCT
    CAGCCAATCC TTAGAACAGA TTCTCCTGGG TATAAAAATG TACCTTCTCA ACAGAAAGCA
    GAAGCCTTAC CTGCAGCCCC TTACCTTTCT GTGTCGTCTG AAACAAACCA TACAACTTCA
    ACTGCTGCTG TTAATCCCAT GGTAAATCTA AATAATGTTC GATTGGAGGA TCCCAACCAT
    GCCCCTTACA GCTCTTATCA ATCACAGGGT GGCCTTTTGA GATCACCAAG CACTGAGGCA
    GCTCATATTA TACTAAGAGA CCAAGAACCT TCATCTTTAC CAGTGCAAAT TGACTCAGCA
    AAGGTTTATA GAAAAGAGGC CTATGCTATG GAAGAGAATC CTAGACAAAA TTATTTGATG
    AAGCAGCAAG TGCTTGGCCA TCAAATTCAA AGACATGAAA AAGAGACCAA TTTAAACTAT
    GATGGCGTGC CAGCATATGC AGAGAAACCA CTGAATAGAG AATATGACCA GTCTTATTGC
    TATGATTCTA CTTCTGATTA TGTAGATCAG TTTCCCCACA ATTATGATTC TCGAATAAAT
    TTTGATGATC ACGTGCCTAC ATATGATGAA CAGTGGGGTT ATTATGATGA CAAACAGTCA
    TATCCCCAGA GACCACCATT TGACAGTAAA CGCAATAGGG ACTTCAATAT TAGACAGCAC
    TCTGATGAAA ACCTTGAACG TAATTACTTT CAGCAGCCTC GATTGGAGGA GCCACCTCCT
    GTTGCTTATG ACAATCGCCC CCGCCCATCC CAAAACTACA AACCAACCCA AGTACATTAT
    GAAGACTCCA CAACAGCAGA GTATGATATG CATATGCGTT ACAAACCTGA ACCTCAAACC
    ATTCCTTCTG TGCCAACATC AAAGTCTTCT GATGCAAAGC CGTATTTTGA TCAACACATA
    AGGGGTTATG AACATAGTCC TACCCAGGTA TTTAACACAA AACCAGGGCA GTATGATACA
    TCTCCTAGAA TGGAGGCAAT GCCACCTCCA CCTGTTCAGA GTAAGCCAGA GCTCCTTTCA
    ACAAGCTCAA AACCTTTGCC TGCCCCTGCT CCTTCCGTTG CTCCTGCAGC AACTGAAGAA
    GACGATGATC CAGCTATGAA GCCACAGTCT GTACTTACTA GAGTGAAAAT GTTTGAGAAC
    AAACGTTCTA ATCAAGGTGA AAAGAACAAG GATATAAATG ATTCCACTCT TGTTAAGGCC
    CCAGATGTTG CACCTAAACC TATACTTAAT CAAGTAAGTG CTACAAAGGC TGCCCCCGTT
    CCACATTATG ATCAGGACAA GGCAACTTAT AGAGTACCTG AACCTCAAAA GCCTCAAGTG
    AAGCCCCCAG AAGATATAAT TCGGGCCAAC CACTATGACC CCGATGAAGA TGAAGAATAC
    TATCGAAAGC AGCTGTCTTA CTTTGATCGA AGAAGCTTTG AAAAGCCCAC ACCCTTGCTG
    TCTGGAAACA TCCTGCCTGA GCCTGCCAAA CCAGCGCAGT CTCAAAGCCA ACTTAGCTTT
    AACAGCTACC CCTCCAAGGG AAAGCCTGCA GATTTGGAGC CACTTGACAG ACCCATTAAT
    GAAAAACGCT ATGATCCAGT TCCACAGGTT ACTTCTTCTA ACCAGTATAC TCAGCCTTCA
    CCAGTGGTTG TAAATCATTC AACACACACT AAACATATGC ATTTAGAAGC CAGCTCTGTG
    TCTGTGGAGG TTCCAAGTTC CACAGTGTCT AAGCCAGAGC CCCCACCTAC TCAGGCCAAG
    CCAACATTGT TCCGACCATC AAACAGAGAG GACACTGTAC AGTCAAACTT CTATCCTCAG
    AAGAGTTTCC CTGAAAAAAG CCCTGTAAAT GGCACTGAAC AGGCTCCAAA AACAGTAACA
    CCATCCTACA ATCGTTTTAC ACCTAAACCT TACACAAATT CAGCGAGACC CTTTGAACGC
    AAGTTTGAAA GCCCAAAATT TAACCACAAC CTCCTGCCAA ATGAAGCACA AGTAAAGCCA
    GAGCTGCCCA CTAAACCTCA GAATTCTCCA CAGCCTGTTT TAAAATCAAA TACGTCAATT
    TCTTCTGAAC TGGATAGCAT ATCCAAGCAA ACGGAGAACA AATCTAAATA TCAACCAAAC
    AATGTTAATC CAGTGCCTAA AGCAATCCCA GTCAGTCCTT CTGCATTGGA AGATGACGAT
    GAAGATGATG CACATACAGT TGTAGCAACA GCTAGAGGCT TCTTTAATAG TAATGGAGGT
    GTGTTAAGTT CCATAGAAAC GGGAGTCAGT ATCATCATAC CCCAAGGAGC AATTCCAGAT
    GGCATTGAGC AAGAAATATA TTTCAAGGTC TGCAGGGATA ACAGTATCCT TCCACCCTTG
    GACAAAGAGA AAGGAGAGAC GCTACTTAGT CCATTAGTAA TGTGCGGACC TCATGGGTTA
    AAATTCGTGA AACCAGTGGA GCTACGCTTA CCACACTGTG CGTCTATGAC TCCTGATGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACACC TCGTCGGGTG ACCCAAAACA CTGGCAGAAC
    AAGTCACTTC CTGGCGACCC CAACTATCTT GTCGGAGCAA ACTGTGTCTC AGTTTTGATA
    GACCACTTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55059
    Clone ID Related Accession (Same CDS sequence) XM_031899167.1
    Accession Version XM_031899167.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGTTTA TAGAAAAGAG GCCTATGCTA TGGAAGAGAA TCCTAGACAA
    AATTATTTGA TGAAGCAGCA AGTGCTTGGC CATCAAATTC AAAGACATGA AAAAGAGACC
    AATTTAAACT ATGATGGCGT GCCAGCATAT GCAGAGAAAC CACTGAATAG AGAATATGAC
    CAGTCTTATT GCTATGATTC TACTTCTGAT TATGTAGATC AGTTTCCCCA CAATTATGAT
    TCTCGAATAA ATTTTGATGA TCACGTGCCT ACATATGATG AACAGTGGGG TTATTATGAT
    GACAAACAGT CATATCCCCA GAGACCACCA TTTGACAGTA AACGCAATAG GGACTTCAAT
    ATTAGACAGC ACTCTGATGA AAACCTTGAA CGTAATTACT TTCAGCAGCC TCGATTGGAG
    GAGCCACCTC CTGTTGCTTA TGACAATCGC CCCCGCCCAT CCCAAAACTA CAAACCAACC
    CAAGTACATT ATGAAGACTC CACAACAGCA GAGTATGATA TGCATATGCG TTACAAACCT
    GAACCTCAAA CCATTCCTTC TGTGCCAACA TCAAAGTCTT CTGATGCAAA GCCGTATTTT
    GATCAACACA TAAGGGGTTA TGAACATAGT CCTACCCAGG TATTTAACAC AAAACCAGGG
    CAGTATGATA CATCTCCTAG AATGGAGGCA ATGCCACCTC CACCTGTTCA GAGTAAGCCA
    GAGCTCCTTT CAACAAGCTC AAAACCTTTG CCTGCCCCTG CTCCTTCCGT TGCTCCTGCA
    GCAACTGAAG AAGACGATGA TCCAGCTATG AAGCCACAGT CTGTACTTAC TAGAGTGAAA
    ATGTTTGAGA ACAAACGTTC TAATCAAGGT GAAAAGAACA AGGATATAAA TGATTCCACT
    CTTGTTAAGG CCCCAGATGT TGCACCTAAA CCTATACTTA ATCAAGTAAG TGCTACAAAG
    GCTGCCCCCG TTCCACATTA TGATCAGGAC AAGGCAACTT ATAGAGTACC TGAACCTCAA
    AAGCCTCAAG TGAAGCCCCC AGAAGATATA ATTCGGGCCA ACCACTATGA CCCCGATGAA
    GATGAAGAAT ACTATCGAAA GCAGCTGTCT TACTTTGATC GAAGAAGCTT TGAAAAGCCC
    ACACCCTTGC TGTCTGGAAA CATCCTGCCT GAGCCTGCCA AACCAGCGCA GTCTCAAAGC
    CAACTTAGCT TTAACAGCTA CCCCTCCAAG GGAAAGCCTG CAGATTTGGA GCCACTTGAC
    AGACCCATTA ATGAAAAACG CTATGATCCA GTTCCACAGG TTACTTCTTC TAACCAGTAT
    ACTCAGCCTT CACCAGTGGT TGTAAATCAT TCAACACACA CTAAACATAT GCATTTAGAA
    GCCAGCTCTG TGTCTGTGGA GGTTCCAAGT TCCACAGTGT CTAAGCCAGA GCCCCCACCT
    ACTCAGGCCA AGCCAACATT GTTCCGACCA TCAAACAGAG AGGACACTGT ACAGTCAAAC
    TTCTATCCTC AGAAGAGTTT CCCTGAAAAA AGCCCTGTAA ATGGCACTGA ACAGGCTCCA
    AAAACAGTAA CACCATCCTA CAATCGTTTT ACACCTAAAC CTTACACAAA TTCAGCGAGA
    CCCTTTGAAC GCAAGTTTGA AAGCCCAAAA TTTAACCACA ACCTCCTGCC AAATGAAGCA
    CAAGTAAAGC CAGAGCTGCC CACTAAACCT CAGAATTCTC CACAGCCTGT TTTAAAATCA
    AATACGTCAA TTTCTTCTGA ACTGGATAGC ATATCCAAGC AAACGGAGAA CAAATCTAAA
    TATCAACCAA ACAATGTTAA TCCAGTGCCT AAAGCAATCC CAGTCAGTCC TTCTGCATTG
    GAAGATGACG ATGAAGATGA TGCACATACA GTTGTAGCAA CAGCTAGAGG CTTCTTTAAT
    AGTAATGGAG GTGTGTTAAG TTCCATAGAA ACGGGAGTCA GTATCATCAT ACCCCAAGGA
    GCAATTCCAG ATGGCATTGA GCAAGAAATA TATTTCAAGG TCTGCAGGGA TAACAGTATC
    CTTCCACCCT TGGACAAAGA GAAAGGAGAG ACGCTACTTA GTCCATTAGT AATGTGCGGA
    CCTCATGGGT TAAAATTCGT GAAACCAGTG GAGCTACGCT TACCACACTG TGCGTCTATG
    ACTCCTGATG GTTGGTCTTT TGCTCTAAAA TCATCCGACA CCTCGTCGGG TGACCCAAAA
    CACTGGCAGA ACAAGTCACT TCCTGGCGAC CCCAACTATC TTGTCGGAGC AAACTGTGTC
    TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755027.1
    CDS25..5268
    Translation

    Target ORF information:

    RefSeq Version XM_031899167.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899167.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGTTTA TAGAAAAGAG GCCTATGCTA TGGAAGAGAA TCCTAGACAA
    AATTATTTGA TGAAGCAGCA AGTGCTTGGC CATCAAATTC AAAGACATGA AAAAGAGACC
    AATTTAAACT ATGATGGCGT GCCAGCATAT GCAGAGAAAC CACTGAATAG AGAATATGAC
    CAGTCTTATT GCTATGATTC TACTTCTGAT TATGTAGATC AGTTTCCCCA CAATTATGAT
    TCTCGAATAA ATTTTGATGA TCACGTGCCT ACATATGATG AACAGTGGGG TTATTATGAT
    GACAAACAGT CATATCCCCA GAGACCACCA TTTGACAGTA AACGCAATAG GGACTTCAAT
    ATTAGACAGC ACTCTGATGA AAACCTTGAA CGTAATTACT TTCAGCAGCC TCGATTGGAG
    GAGCCACCTC CTGTTGCTTA TGACAATCGC CCCCGCCCAT CCCAAAACTA CAAACCAACC
    CAAGTACATT ATGAAGACTC CACAACAGCA GAGTATGATA TGCATATGCG TTACAAACCT
    GAACCTCAAA CCATTCCTTC TGTGCCAACA TCAAAGTCTT CTGATGCAAA GCCGTATTTT
    GATCAACACA TAAGGGGTTA TGAACATAGT CCTACCCAGG TATTTAACAC AAAACCAGGG
    CAGTATGATA CATCTCCTAG AATGGAGGCA ATGCCACCTC CACCTGTTCA GAGTAAGCCA
    GAGCTCCTTT CAACAAGCTC AAAACCTTTG CCTGCCCCTG CTCCTTCCGT TGCTCCTGCA
    GCAACTGAAG AAGACGATGA TCCAGCTATG AAGCCACAGT CTGTACTTAC TAGAGTGAAA
    ATGTTTGAGA ACAAACGTTC TAATCAAGGT GAAAAGAACA AGGATATAAA TGATTCCACT
    CTTGTTAAGG CCCCAGATGT TGCACCTAAA CCTATACTTA ATCAAGTAAG TGCTACAAAG
    GCTGCCCCCG TTCCACATTA TGATCAGGAC AAGGCAACTT ATAGAGTACC TGAACCTCAA
    AAGCCTCAAG TGAAGCCCCC AGAAGATATA ATTCGGGCCA ACCACTATGA CCCCGATGAA
    GATGAAGAAT ACTATCGAAA GCAGCTGTCT TACTTTGATC GAAGAAGCTT TGAAAAGCCC
    ACACCCTTGC TGTCTGGAAA CATCCTGCCT GAGCCTGCCA AACCAGCGCA GTCTCAAAGC
    CAACTTAGCT TTAACAGCTA CCCCTCCAAG GGAAAGCCTG CAGATTTGGA GCCACTTGAC
    AGACCCATTA ATGAAAAACG CTATGATCCA GTTCCACAGG TTACTTCTTC TAACCAGTAT
    ACTCAGCCTT CACCAGTGGT TGTAAATCAT TCAACACACA CTAAACATAT GCATTTAGAA
    GCCAGCTCTG TGTCTGTGGA GGTTCCAAGT TCCACAGTGT CTAAGCCAGA GCCCCCACCT
    ACTCAGGCCA AGCCAACATT GTTCCGACCA TCAAACAGAG AGGACACTGT ACAGTCAAAC
    TTCTATCCTC AGAAGAGTTT CCCTGAAAAA AGCCCTGTAA ATGGCACTGA ACAGGCTCCA
    AAAACAGTAA CACCATCCTA CAATCGTTTT ACACCTAAAC CTTACACAAA TTCAGCGAGA
    CCCTTTGAAC GCAAGTTTGA AAGCCCAAAA TTTAACCACA ACCTCCTGCC AAATGAAGCA
    CAAGTAAAGC CAGAGCTGCC CACTAAACCT CAGAATTCTC CACAGCCTGT TTTAAAATCA
    AATACGTCAA TTTCTTCTGA ACTGGATAGC ATATCCAAGC AAACGGAGAA CAAATCTAAA
    TATCAACCAA ACAATGTTAA TCCAGTGCCT AAAGCAATCC CAGTCAGTCC TTCTGCATTG
    GAAGATGACG ATGAAGATGA TGCACATACA GTTGTAGCAA CAGCTAGAGG CTTCTTTAAT
    AGTAATGGAG GTGTGTTAAG TTCCATAGAA ACGGGAGTCA GTATCATCAT ACCCCAAGGA
    GCAATTCCAG ATGGCATTGA GCAAGAAATA TATTTCAAGG TCTGCAGGGA TAACAGTATC
    CTTCCACCCT TGGACAAAGA GAAAGGAGAG ACGCTACTTA GTCCATTAGT AATGTGCGGA
    CCTCATGGGT TAAAATTCGT GAAACCAGTG GAGCTACGCT TACCACACTG TGCGTCTATG
    ACTCCTGATG GTTGGTCTTT TGCTCTAAAA TCATCCGACA CCTCGTCGGG TGACCCAAAA
    CACTGGCAGA ACAAGTCACT TCCTGGCGAC CCCAACTATC TTGTCGGAGC AAACTGTGTC
    TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55061
    Clone ID Related Accession (Same CDS sequence) XM_031899169.1
    Accession Version XM_031899169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGTTTA TAGAAAAGAG GCCTATGCTA TGGAAGAGAA TCCTAGACAA
    AATTATTTGA TGAAGCAGCA AGTGCTTGGC CATCAAATTC AAAGACATGA AAAAGAGACC
    AATTTAAACT ATGATGGCGT GCCAGCATAT GCAGAGAAAC CACTGAATAG AGAATATGAC
    CAGTCTTATT GCTATGATTC TACTTCTGAT TATGTAGATC AGTTTCCCCA CAATTATGAT
    TCTCGAATAA ATTTTGATGA TCACGTGCCT ACATATGATG AACAGTGGGG TTATTATGAT
    GACAAACAGT CATATCCCCA GAGACCACCA TTTGACAGTA AACGCAATAG GGACTTCAAT
    ATTAGACAGC ACTCTGATGA AAACCTTGAA CGTAATTACT TTCAGCAGCC TCGATTGGAG
    GAGCCACCTC CTGTTGCTTA TGACAATCGC CCCCGCCCAT CCCAAAACTA CAAACCAACC
    CAAGTACATT ATGAAGACTC CACAACAGCA GAGTATGATA TGCATATGCG TTACAAACCT
    GAACCTCAAA CCATTCCTTC TGTGCCAACA TCAAAGTCTT CTGATGCAAA GCCGTATTTT
    GATCAACACA TAAGGGGTTA TGAACATAGT CCTACCCAGG TATTTAACAC AAAACCAGGG
    CAGTATGATA CATCTCCTAG AATGGAGGCA ATGCCACCTC CACCTGTTCA GAGTAAGCCA
    GAGCTCCTTT CAACAAGCTC AAAACCTTTG CCTGCCCCTG CTCCTTCCGT TGCTCCTGCA
    GCAACTGAAG AAGACGATGA TCCAGCTATG AAGCCACAGT CTGTACTTAC TAGAGTGAAA
    ATGTTTGAGA ACAAACGTTC TAATCAAGGT GAAAAGAACA AGGATATAAA TGATTCCACT
    CTTGTTAAGG CCCCAGATGT TGCACCTAAA CCTATACTTA ATCAAGTAAG TGCTACAAAG
    GCTGCCCCCG TTCCACATTA TGATCAGGAC AAGGCAACTT ATAGAGTACC TGAACCTCAA
    AAGCCTCAAG TGAAGCCCCC AGAAGATATA ATTCGGGCCA ACCACTATGA CCCCGATGAA
    GATGAAGAAT ACTATCGAAA GCAGCTGTCT TACTTTGATC GAAGAAGCTT TGAAAAGCCC
    ACACCCTTGC TGTCTGGAAA CATCCTGCCT GAGCCTGCCA AACCAGCGCA GTCTCAAAGC
    CAACTTAGCT TTAACAGCTA CCCCTCCAAG GGAAAGCCTG CAGATTTGGA GCCACTTGAC
    AGACCCATTA ATGAAAAACG CTATGATCCA GTTCCACAGG TTACTTCTTC TAACCAGTAT
    ACTCAGCCTT CACCAGTGGT TGTAAATCAT TCAACACACA CTAAACATAT GCATTTAGAA
    GCCAGCTCTG TGTCTGTGGA GGTTCCAAGT TCCACAGTGT CTAAGCCAGA GCCCCCACCT
    ACTCAGGCCA AGCCAACATT GTTCCGACCA TCAAACAGAG AGGACACTGT ACAGTCAAAC
    TTCTATCCTC AGAAGAGTTT CCCTGAAAAA AGCCCTGTAA ATGGCACTGA ACAGGCTCCA
    AAAACAGTAA CACCATCCTA CAATCGTTTT ACACCTAAAC CTTACACAAA TTCAGCGAGA
    CCCTTTGAAC GCAAGTTTGA AAGCCCAAAA TTTAACCACA ACCTCCTGCC AAATGAAGCA
    CAAGTAAAGC CAGAGCTGCC CACTAAACCT CAGAATTCTC CACAGCCTGT TTTAAAATCA
    AATACGTCAA TTTCTTCTGA ACTGGATAGC ATATCCAAGC AAACGGAGAA CAAATCTAAA
    TATCAACCAA ACAATGTTAA TCCAGTGCCT AAAGCAATCC CAGTCAGTCC TTCTGCATTG
    GAAGATGACG ATGAAGATGA TGCACATACA GTTGTAGCAA CAGCTAGAGG CTTCTTTAAT
    AGTAATGGAG GTGTGTTAAG TTCCATAGAA ACGGGAGTCA GTATCATCAT ACCCCAAGGA
    GCAATTCCAG ATGGCATTGA GCAAGAAATA TATTTCAAGG TCTGCAGGGA TAACAGTATC
    CTTCCACCCT TGGACAAAGA GAAAGGAGAG ACGCTACTTA GTCCATTAGT AATGTGCGGA
    CCTCATGGGT TAAAATTCGT GAAACCAGTG GAGCTACGCT TACCACACTG TGACCCAAAA
    CACTGGCAGA ACAAGTCACT TCCTGGCGAC CCCAACTATC TTGTCGGAGC AAACTGTGTC
    TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755029.1
    CDS25..5208
    Translation

    Target ORF information:

    RefSeq Version XM_031899169.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis tight junction protein 1 (tjp1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAAATACC AGAAATACCT GACTGTGCTG CAGATGGCGA TCGGAGTGAC TCCTTCCAAC 
    AGAGGGAATA TACTGCCTTA CAAGCGGAGG CTGTGGGTCA CTCCGTCATC TGAGAATCCC
    AATGCTGCTG CATCGAGTAC GAGTCAGGGA AAACCATCTC TAAGGAGAAT AAAGGGACGG
    ATCCACAGAA GCAAAAGCTT GGATAGCGTT GACCTCTGCG ATTTTAATAA TACTGTAATG
    GAAGAAACTG CAATATGGGA ACAGCAAACA GTTACTCTCC ACAGGGCCCC TGGATTTGGA
    TTTGGCATTG CAATATCTGG AGGCAGAGAT AACCCACATT TTCAAAGTGG GGAAACATCA
    ATTGTCATCT CTGATGTTCT TAAAGGAGGG CCTGCAGAAG GTTTGCTTCA GGAAAATGAT
    CGTGTTGCAA TGGTCAATGG GGTTTCTATG GATAATGTCG AGCATGCCTT TGCTGTTCAG
    CAGTTGAGGA AAAGTGGAAA AAATGCCAAA ATTACTATCC GGCGGAAGAA AAAAGTGCAA
    CTTCCAGTAG TTCGTTCTGA CCCAGACCCA GTGTCTGAGA ATGAAGAGGA CAGTTATGAG
    GAGGATGTGC CTGACCAGAG AAGTGCCCAC AGTGGTCCTG GTGCCAGCAG GAGAAGTGAA
    AAAAGTTGGG TGAGGGACAG AAGTGCAAGC AGAGAAAGAA GTTTATCACC AAGATCAGAC
    AGAAGATCTG TTTCTTCCAG TCAACCTGCT AAACCTACCA AAGTGACCTT GGTGAAATCT
    AGAAAAAATG AAGAATATGG TTTAAGGTTG GCTAGCCACA TATTTGTAAA AGAAATTTCC
    CAAGACAGTC TTGCGGCAAG AGATGGAAAT ATTCAAGAAG GGGATGTTGT GCTTAAGATT
    AATGGCACAG TCACAGAAAA CATGTCTTTG GCTGATGCAA AAACATTAAT AGAAAGGTCG
    AAGGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGAG CAACTCTACT TAATGTCCCA
    GATCTAGATG ACAGTCTCCA TTCTGCAAAT GCTTCAGAAA GAGATGACAT TTCAGAAATT
    CAGTCGCTAA CATCAGATCA TTCCAACCGT TCACATGATA AACCTCGCCG TAGCCGTTCA
    CGGTCACCGG ACCAGAGGTC AGAGCCTTCA GACCATTCCA GACATTCTCC ACAACAGCAC
    AGCAACAGCA GTAGTCGAAG TCGAGAGGAT GAAAGAGTTT CAAAGCCTGG TGCAATATCT
    ACTCCGGTGA AGAATGTCAA TAGTGTACCT CTTTCCAGAA CTGCAGAAGA ACCCTTTTTA
    GAAAAGGCAG ATAAACAAAC ACCTCCTCTT CCAGAGCCAA AACCTGTATA TGCCCAGCCA
    GGACAACCAG ATGTGGATTT GCCAGTCAGC CCATCTGATG GACCTTTCCC TAATTCTACA
    CATGAAGATG GCATGCTTCG GCCAAGCATG AAACTGGTGA AGTTCAGGAA AGGAGACAGT
    GTAGGCCTTC GTTTGGCTGG TGGTAATGAT GTGGGAATTT TTGTTGCTGG TGTATTAGAC
    GACAGTCCTG CCGCAAAAGA AGGTTTGGAG GAAGGGGACC AGATACTCAG AGTTAACAAT
    GTGGATTTCA CCAATATAAT AAGGGAAGAA GCGGTACTGT TCCTTCTTGA TCTGCCCAAA
    GGAGAAGAGG TTACCATTCT TGCTCAGAAA AAGAAAGATG TGTATCGCCG CATTGTTGAG
    TCAGATGTTG GGGATTCATT TTATATAAGA ACTCACTTTG AATATGAGAA AGAGTCACCA
    TACGGGCTAA GTTTCAACAA AGGAGAGGTC TTTCGAGTTG TGGATACTCT TTACAATGGC
    AAACTAGGGT CATGGTTGGC CATTAGAATT GGTAGAAATC ACAAGGAGGT GGAAAGAGGG
    ATAATCCCCA ACAAGAATAG GGCTGAGCAA CTGGCTAGTG TACAGTACAC TCTTCCTAAA
    ACTGCTGGTG GAGATCGTGC AGACTTCTGG AGATTCCGTG GCCTTCGTAG TTCCAAGCGA
    AATCTACGGA AAAGCAGAGA GGATCTTTCT GCGCAACCTG TTCAAACAAA GTTCCCTGCT
    TATGAGAGAG TCGTCTTGAG AGAAGCTGGA TTTCTCAGGC CTGTGGTTAT TTTTGGCCCT
    ATTGCTGACG TTGCAAGAGA AAAGTTGGCA AGAGAAGAAC CAGATATTTA TGAAGTAGCA
    AGAAGTGAAC CCAGAGATGC AGGTACTGAC CAGCGAAGTT CTGGAATTAT CCGCCTTCAT
    ACTATTAAAC AGATTATTGA TAGGGATAAG CATGCTCTTC TAGATGTGAC TCCAAATGCT
    GTGGACCGCT TAAATTATGC ACAATGGTAT CCGATAGTAG TGTTTCTTAA CCCAGACTCT
    AAACAAGGTG TGAAAACAAT GAGAATGAGG TTATGCCCAG AATCACGCAA AAGTGCCAGG
    AAACTATATG AACGTTCACA CAAACTACGC AAAAACAATC ACCATCTTTT CACAACTACA
    ATAAATCTCA ACTCCATGAA TGATGGCTGG TATGGTGGCC TTAAAGAGGC AGCACAGCAG
    CAACAGAACC AGCTAGTTTG GGTCTCAGAG GGCAAAGCAG AAGGTGGCAC AGATGATGAC
    CTTGATTTGC ATGACGACCG TCTTTCCTAC CTATCAGCAC CAGGTAGTGA ATATTCAATG
    TATAGCACTG ATAGTAGGCA CACATCAGAC TATGAAGACA CAGACACAGA AGGAGGGGCT
    TACACTGACC AGGACCTGGA TGAGACACTT AATGATGAGG CTGGGACTCC ACCAGAATCT
    GCAATCACAC GATCTTCTGA ACCAGTCAGA GAAGACACAT CAGGGATGCA AGAAAACCCA
    GCATACCCAA CATATCCACC TCAGCCAATC CTTAGAACAG ATTCTCCTGG GTATAAAAAT
    GTACCTTCTC AACAGGTTTA TAGAAAAGAG GCCTATGCTA TGGAAGAGAA TCCTAGACAA
    AATTATTTGA TGAAGCAGCA AGTGCTTGGC CATCAAATTC AAAGACATGA AAAAGAGACC
    AATTTAAACT ATGATGGCGT GCCAGCATAT GCAGAGAAAC CACTGAATAG AGAATATGAC
    CAGTCTTATT GCTATGATTC TACTTCTGAT TATGTAGATC AGTTTCCCCA CAATTATGAT
    TCTCGAATAA ATTTTGATGA TCACGTGCCT ACATATGATG AACAGTGGGG TTATTATGAT
    GACAAACAGT CATATCCCCA GAGACCACCA TTTGACAGTA AACGCAATAG GGACTTCAAT
    ATTAGACAGC ACTCTGATGA AAACCTTGAA CGTAATTACT TTCAGCAGCC TCGATTGGAG
    GAGCCACCTC CTGTTGCTTA TGACAATCGC CCCCGCCCAT CCCAAAACTA CAAACCAACC
    CAAGTACATT ATGAAGACTC CACAACAGCA GAGTATGATA TGCATATGCG TTACAAACCT
    GAACCTCAAA CCATTCCTTC TGTGCCAACA TCAAAGTCTT CTGATGCAAA GCCGTATTTT
    GATCAACACA TAAGGGGTTA TGAACATAGT CCTACCCAGG TATTTAACAC AAAACCAGGG
    CAGTATGATA CATCTCCTAG AATGGAGGCA ATGCCACCTC CACCTGTTCA GAGTAAGCCA
    GAGCTCCTTT CAACAAGCTC AAAACCTTTG CCTGCCCCTG CTCCTTCCGT TGCTCCTGCA
    GCAACTGAAG AAGACGATGA TCCAGCTATG AAGCCACAGT CTGTACTTAC TAGAGTGAAA
    ATGTTTGAGA ACAAACGTTC TAATCAAGGT GAAAAGAACA AGGATATAAA TGATTCCACT
    CTTGTTAAGG CCCCAGATGT TGCACCTAAA CCTATACTTA ATCAAGTAAG TGCTACAAAG
    GCTGCCCCCG TTCCACATTA TGATCAGGAC AAGGCAACTT ATAGAGTACC TGAACCTCAA
    AAGCCTCAAG TGAAGCCCCC AGAAGATATA ATTCGGGCCA ACCACTATGA CCCCGATGAA
    GATGAAGAAT ACTATCGAAA GCAGCTGTCT TACTTTGATC GAAGAAGCTT TGAAAAGCCC
    ACACCCTTGC TGTCTGGAAA CATCCTGCCT GAGCCTGCCA AACCAGCGCA GTCTCAAAGC
    CAACTTAGCT TTAACAGCTA CCCCTCCAAG GGAAAGCCTG CAGATTTGGA GCCACTTGAC
    AGACCCATTA ATGAAAAACG CTATGATCCA GTTCCACAGG TTACTTCTTC TAACCAGTAT
    ACTCAGCCTT CACCAGTGGT TGTAAATCAT TCAACACACA CTAAACATAT GCATTTAGAA
    GCCAGCTCTG TGTCTGTGGA GGTTCCAAGT TCCACAGTGT CTAAGCCAGA GCCCCCACCT
    ACTCAGGCCA AGCCAACATT GTTCCGACCA TCAAACAGAG AGGACACTGT ACAGTCAAAC
    TTCTATCCTC AGAAGAGTTT CCCTGAAAAA AGCCCTGTAA ATGGCACTGA ACAGGCTCCA
    AAAACAGTAA CACCATCCTA CAATCGTTTT ACACCTAAAC CTTACACAAA TTCAGCGAGA
    CCCTTTGAAC GCAAGTTTGA AAGCCCAAAA TTTAACCACA ACCTCCTGCC AAATGAAGCA
    CAAGTAAAGC CAGAGCTGCC CACTAAACCT CAGAATTCTC CACAGCCTGT TTTAAAATCA
    AATACGTCAA TTTCTTCTGA ACTGGATAGC ATATCCAAGC AAACGGAGAA CAAATCTAAA
    TATCAACCAA ACAATGTTAA TCCAGTGCCT AAAGCAATCC CAGTCAGTCC TTCTGCATTG
    GAAGATGACG ATGAAGATGA TGCACATACA GTTGTAGCAA CAGCTAGAGG CTTCTTTAAT
    AGTAATGGAG GTGTGTTAAG TTCCATAGAA ACGGGAGTCA GTATCATCAT ACCCCAAGGA
    GCAATTCCAG ATGGCATTGA GCAAGAAATA TATTTCAAGG TCTGCAGGGA TAACAGTATC
    CTTCCACCCT TGGACAAAGA GAAAGGAGAG ACGCTACTTA GTCCATTAGT AATGTGCGGA
    CCTCATGGGT TAAAATTCGT GAAACCAGTG GAGCTACGCT TACCACACTG TGACCCAAAA
    CACTGGCAGA ACAAGTCACT TCCTGGCGAC CCCAACTATC TTGTCGGAGC AAACTGTGTC
    TCAGTTTTGA TAGACCACTT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.