phka2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following phka2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa37159 XM_012957497.3
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa42688 XM_018091486.1
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa42689 XM_018091487.1
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa52143 XM_012957496.3
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52143 XM_012957495.3
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52144 XM_004911663.4
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52145 XM_031896750.1
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa05606 XM_004911663.3
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa37158 XM_012957496.2
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa37158 XM_012957495.2
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa37160 XM_012957498.2
Latest version!
Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OXa37159 XM_012957497.2 Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa37159
    Clone ID Related Accession (Same CDS sequence) XM_012957497.2 , XM_012957497.3
    Accession Version XM_012957497.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812951.1
    CDS476..4123
    Translation

    Target ORF information:

    RefSeq Version XM_012957497.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42688
    Clone ID Related Accession (Same CDS sequence) XM_018091486.1
    Accession Version XM_018091486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGATTA TGGAATGTGG
    GAGCGAGGAG ACAAAACAAA TCAAGGAATT CCTGAGCTAA ATGCAAGCTC TGTGGGCATG
    GCAAAGGCTG CTCTGGAAGC TATAGATGAA CTTGATCTGT TTGGCGCTCA TGGGGGACCC
    AAATCTGTGA TTCATGTTCT ACCTGATGAT GTAGAACACT GTCAGTCAAT CCTTTATTCA
    ATGTTGCCTC GGGCCTCCAC CTCCAAAGAA ATTGATGCTG GCCTCCTCTC CATCATTTCA
    TATCCTGCTT TTGCTGTTGA GGACATAAAT CTGGTTAATG TGACCAAAAA TGAAATAATC
    ACTAAACTCC AGGGAAGATA TGGCTGCTGT CGCTTTCTCA GAGATGGCTT TAAAACACCA
    AGAGAGGACC CCACCAGATT GCACTATGAC TCTACTGAAC TCAAACTCTT TGAGAATATT
    GAATGTGAAT GGCCTTTGTT CTGGACATAT TTACTTATTG ATGGAATTTT TAATGGGGAT
    CAGGTTCAGA TGCAAGAATA TCGGGAAGCT CTGGAGGGAA TTCTCATAAG AGGAAAGAAT
    GGAATTCGTC TTGTGCCAGA ACTTTATGCT GTTCCTACTG ACAAGATAGA AGAAGAGTAC
    CATAACCCGC ATACAGTTGA CCGCAGACCT TTGGGGAAGA TACCTCACCT ATGGGGACAA
    TCACTGTACA TACTGGGCTC TCTTTTAGCT GAGGGATTTC TAGCACCAGG AGAAGTAGAT
    CCGTTGAACA GAAGATTCTC TACAGTTTTT AAGCCTGATG TTGTGGTGCA AGTTTCAGTT
    CTAGCAGAGT CTAAAGAGAT AAAGAAAATG CTCAAAAACC ATGGCCTTGA AGTTCAGAGC
    TTTAGTGATG TCCGGCCAAT CAGAGTGCAG CCAGCTAGGA TTTTGAGCCA CCTGTATGCC
    AAGCTTGGGC ATAATAAAGC CATGAAGCTT AGTGGCCGCC CATACCGTCA CATAGGTGTT
    CTAGGGACGT CTAAACTGTA CATGATCAGG AACCAGATCT TTACTTTTAC ACCCCAGTTT
    ACAGATCAGC ACCACTTCTA TCTAGCCCTC GACAGCAGAA TGATTGTGGA GATGCTTAGA
    ACTGAACTGG CCTATTTAAC TTCCTGCTGG AGGATGACTG GGAGACCAAC GCTAACCTTT
    CCCATTACCC ATTCTATGCT CAATGAAGAT GGCTCTGATA TTGATCCTGC TATTCTTTCT
    ACAATTAGAA AGATGGAAGA TGGATATTTT GGTGGCGCAA GGGTGAAGCT TGGAAAGTTA
    TCAGATTTTC TTACTACATC TTTCTACACT CATCTAAGTT TTCTGGATTC TGACTGTGAT
    GGAAAGCTTT TTGATGATGT AGATGACGAA TGTTATAGTC CCGTCAATGA CTATGAAGTG
    GAGTTATTTT CGAATACAGA GTGTGAGAAA GGTAATATAA CTAAAGAGGA AGTTCAGGAT
    GAGCTTGATC AGTATATAAA TCAACTGCAG CAAAGCATGG CTTCAACATC TTACCTCCCT
    CCTATCTCCA GTCACTCCGG CACATACCAT GCCTTCAGCA CACTCCATTC CACCCGGGAT
    ATTCTATCGA TAATGGCTAA ATCTAAAGGC CTGGATGTAC CAAATTCCTC CCTATTTCTT
    CCTGCTAAAA ATCTGAGTGC TCATCGGAGA TCTTTAAATC TGTTAGAGGT CCCACAACTA
    CACAAACACC AGAATCATGA AACTGAATTA AATTTACCAA AAGATGCCCA TGGGGATATT
    GACTGCCACA AGCTGCTGGA TAAACTCAAG GAATGCCCAA CTCTTCAAGA CCAAGCCGAT
    ATTCTTTATA TACTGTGTGT GATAAAAGGA CTGGACTGGG ATACAGGGCT GAATGGACAG
    TGTGGTGTGT CGGTACATTT GCTGCTAAAT GAGTTATATA GCAAGGCAGG ACTCAATCGG
    GAATGGGGTT TGATCCGTTA CATCTCTGGC CTTCTGAAAA AGAGAGTGGA GGTCCTTGCT
    GAGGCTTGTA CTGACATCTT GTCGCACCAT AAGCAGCTTA CTGTTGGCCT TCCCCCGGAA
    CCCCGAGAGA AAACCATCAC AGCACCCCTT CCTCCGGAAC AGCTGGCAGC CCTGATTTAT
    GAAGCGAGTG GACAAGATAT TAGCATTGCT GTACTTACAC AGGAAATAAT GCTTTATCTG
    GCTATGTATG TCAGATCTCA GCCAAGTCTC TTTGGAGAGA TGCTGAGGCT GCGGATTGGA
    CTCATAATGC AGGTGATGGC AACTGAACTT GCACGCAGCC TAAACTGCTC TGGTGAAGAA
    GCTTCAGAAA GTTTGATGAG CCTCAGCCCT TTTGATATGA AGAACCTACT TCACCATATC
    CTGAGTGGTA AAGAGTTTGG CGTAGAACGA AGCATGCGTC CAATTGAATC ATCGGCCACT
    AGCCCCGCTA TATCTATCCA CGAAATGGGA CACACAGGAG CAACGAAAAC AGAAAGGAGC
    GGCATCAACA AGCTCAGGAA TGAAATGAAA CAAATGGATA GGAGGTTTAG CCAAGATGAT
    CAGTTTGGGC ATTCTGTCGC CAGCAGCATC CATTCCTCCA GGTCTACGAG ATGCAGCAGC
    CCTTCTTCTC CCACGGGTAC TCTCTCCCCA ACGGGATTTG TTGGACCCCA TCTTGTTTTC
    GATGACCACC AAGGTCAGTG GTTGAGAAGG AGAAGGCTTG ATGGGGCTAT AAACCGCGTA
    CCGGTCGGAT TCTACCAGAG AGTATGGAAT GTTTTGCAAA AGTGCCATGG TTTATCAATT
    GATGGGTATG TTCTTCCTTC CTCGAGCACA CGAGAGATGA CTGCTTGTGA GATCAAGTTT
    GCTGTCCATG TGGAATCTGT TCTGAATCAT GTTCCCCAGC CTGAATACAG GCAGCTGCTA
    GTGGAAGCTA TAAATGTGCT CACCTTGGTG TCAGACATGG AAGTGGATAG CTTTGGTAGC
    ATTATCCACG TCGACCGCAT TGTGCACTCT GCCAATGACC TCTTCCTTCA GGACCAGAAA
    GCCCTCGGTG CTGCAGATTT CTACCTTGAC CTGGATCAAG CTACGGGAAT CTGCAACTTC
    TTCTATGACA GCGCTCCAAG TGGAGCCTAT GGCACAATGA CTTACCTAAC AAAAGCAGTA
    GCCATCAGCT TACAGGATTT CTTACCAAAT ACCAGCTGTT CAATGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946975.1
    CDS499..3969
    Translation

    Target ORF information:

    RefSeq Version XM_018091486.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGATTA TGGAATGTGG
    GAGCGAGGAG ACAAAACAAA TCAAGGAATT CCTGAGCTAA ATGCAAGCTC TGTGGGCATG
    GCAAAGGCTG CTCTGGAAGC TATAGATGAA CTTGATCTGT TTGGCGCTCA TGGGGGACCC
    AAATCTGTGA TTCATGTTCT ACCTGATGAT GTAGAACACT GTCAGTCAAT CCTTTATTCA
    ATGTTGCCTC GGGCCTCCAC CTCCAAAGAA ATTGATGCTG GCCTCCTCTC CATCATTTCA
    TATCCTGCTT TTGCTGTTGA GGACATAAAT CTGGTTAATG TGACCAAAAA TGAAATAATC
    ACTAAACTCC AGGGAAGATA TGGCTGCTGT CGCTTTCTCA GAGATGGCTT TAAAACACCA
    AGAGAGGACC CCACCAGATT GCACTATGAC TCTACTGAAC TCAAACTCTT TGAGAATATT
    GAATGTGAAT GGCCTTTGTT CTGGACATAT TTACTTATTG ATGGAATTTT TAATGGGGAT
    CAGGTTCAGA TGCAAGAATA TCGGGAAGCT CTGGAGGGAA TTCTCATAAG AGGAAAGAAT
    GGAATTCGTC TTGTGCCAGA ACTTTATGCT GTTCCTACTG ACAAGATAGA AGAAGAGTAC
    CATAACCCGC ATACAGTTGA CCGCAGACCT TTGGGGAAGA TACCTCACCT ATGGGGACAA
    TCACTGTACA TACTGGGCTC TCTTTTAGCT GAGGGATTTC TAGCACCAGG AGAAGTAGAT
    CCGTTGAACA GAAGATTCTC TACAGTTTTT AAGCCTGATG TTGTGGTGCA AGTTTCAGTT
    CTAGCAGAGT CTAAAGAGAT AAAGAAAATG CTCAAAAACC ATGGCCTTGA AGTTCAGAGC
    TTTAGTGATG TCCGGCCAAT CAGAGTGCAG CCAGCTAGGA TTTTGAGCCA CCTGTATGCC
    AAGCTTGGGC ATAATAAAGC CATGAAGCTT AGTGGCCGCC CATACCGTCA CATAGGTGTT
    CTAGGGACGT CTAAACTGTA CATGATCAGG AACCAGATCT TTACTTTTAC ACCCCAGTTT
    ACAGATCAGC ACCACTTCTA TCTAGCCCTC GACAGCAGAA TGATTGTGGA GATGCTTAGA
    ACTGAACTGG CCTATTTAAC TTCCTGCTGG AGGATGACTG GGAGACCAAC GCTAACCTTT
    CCCATTACCC ATTCTATGCT CAATGAAGAT GGCTCTGATA TTGATCCTGC TATTCTTTCT
    ACAATTAGAA AGATGGAAGA TGGATATTTT GGTGGCGCAA GGGTGAAGCT TGGAAAGTTA
    TCAGATTTTC TTACTACATC TTTCTACACT CATCTAAGTT TTCTGGATTC TGACTGTGAT
    GGAAAGCTTT TTGATGATGT AGATGACGAA TGTTATAGTC CCGTCAATGA CTATGAAGTG
    GAGTTATTTT CGAATACAGA GTGTGAGAAA GGTAATATAA CTAAAGAGGA AGTTCAGGAT
    GAGCTTGATC AGTATATAAA TCAACTGCAG CAAAGCATGG CTTCAACATC TTACCTCCCT
    CCTATCTCCA GTCACTCCGG CACATACCAT GCCTTCAGCA CACTCCATTC CACCCGGGAT
    ATTCTATCGA TAATGGCTAA ATCTAAAGGC CTGGATGTAC CAAATTCCTC CCTATTTCTT
    CCTGCTAAAA ATCTGAGTGC TCATCGGAGA TCTTTAAATC TGTTAGAGGT CCCACAACTA
    CACAAACACC AGAATCATGA AACTGAATTA AATTTACCAA AAGATGCCCA TGGGGATATT
    GACTGCCACA AGCTGCTGGA TAAACTCAAG GAATGCCCAA CTCTTCAAGA CCAAGCCGAT
    ATTCTTTATA TACTGTGTGT GATAAAAGGA CTGGACTGGG ATACAGGGCT GAATGGACAG
    TGTGGTGTGT CGGTACATTT GCTGCTAAAT GAGTTATATA GCAAGGCAGG ACTCAATCGG
    GAATGGGGTT TGATCCGTTA CATCTCTGGC CTTCTGAAAA AGAGAGTGGA GGTCCTTGCT
    GAGGCTTGTA CTGACATCTT GTCGCACCAT AAGCAGCTTA CTGTTGGCCT TCCCCCGGAA
    CCCCGAGAGA AAACCATCAC AGCACCCCTT CCTCCGGAAC AGCTGGCAGC CCTGATTTAT
    GAAGCGAGTG GACAAGATAT TAGCATTGCT GTACTTACAC AGGAAATAAT GCTTTATCTG
    GCTATGTATG TCAGATCTCA GCCAAGTCTC TTTGGAGAGA TGCTGAGGCT GCGGATTGGA
    CTCATAATGC AGGTGATGGC AACTGAACTT GCACGCAGCC TAAACTGCTC TGGTGAAGAA
    GCTTCAGAAA GTTTGATGAG CCTCAGCCCT TTTGATATGA AGAACCTACT TCACCATATC
    CTGAGTGGTA AAGAGTTTGG CGTAGAACGA AGCATGCGTC CAATTGAATC ATCGGCCACT
    AGCCCCGCTA TATCTATCCA CGAAATGGGA CACACAGGAG CAACGAAAAC AGAAAGGAGC
    GGCATCAACA AGCTCAGGAA TGAAATGAAA CAAATGGATA GGAGGTTTAG CCAAGATGAT
    CAGTTTGGGC ATTCTGTCGC CAGCAGCATC CATTCCTCCA GGTCTACGAG ATGCAGCAGC
    CCTTCTTCTC CCACGGGTAC TCTCTCCCCA ACGGGATTTG TTGGACCCCA TCTTGTTTTC
    GATGACCACC AAGGTCAGTG GTTGAGAAGG AGAAGGCTTG ATGGGGCTAT AAACCGCGTA
    CCGGTCGGAT TCTACCAGAG AGTATGGAAT GTTTTGCAAA AGTGCCATGG TTTATCAATT
    GATGGGTATG TTCTTCCTTC CTCGAGCACA CGAGAGATGA CTGCTTGTGA GATCAAGTTT
    GCTGTCCATG TGGAATCTGT TCTGAATCAT GTTCCCCAGC CTGAATACAG GCAGCTGCTA
    GTGGAAGCTA TAAATGTGCT CACCTTGGTG TCAGACATGG AAGTGGATAG CTTTGGTAGC
    ATTATCCACG TCGACCGCAT TGTGCACTCT GCCAATGACC TCTTCCTTCA GGACCAGAAA
    GCCCTCGGTG CTGCAGATTT CTACCTTGAC CTGGATCAAG CTACGGGAAT CTGCAACTTC
    TTCTATGACA GCGCTCCAAG TGGAGCCTAT GGCACAATGA CTTACCTAAC AAAAGCAGTA
    GCCATCAGCT TACAGGATTT CTTACCAAAT ACCAGCTGTT CAATGCAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42689
    Clone ID Related Accession (Same CDS sequence) XM_018091487.1
    Accession Version XM_018091487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGGAGC GAGGAGACAA AACAAATCAA GGAATTCCTG AGCTAAATGC AAGCTCTGTG 
    GGCATGGCAA AGGCTGCTCT GGAAGCTATA GATGAACTTG ATCTGTTTGG CGCTCATGGG
    GGACCCAAAT CTGTGATTCA TGTTCTACCT GATGATGTAG AACACTGTCA GTCAATCCTT
    TATTCAATGT TGCCTCGGGC CTCCACCTCC AAAGAAATTG ATGCTGGCCT CCTCTCCATC
    ATTTCATATC CTGCTTTTGC TGTTGAGGAC ATAAATCTGG TTAATGTGAC CAAAAATGAA
    ATAATCACTA AACTCCAGGG AAGATATGGC TGCTGTCGCT TTCTCAGAGA TGGCTTTAAA
    ACACCAAGAG AGGACCCCAC CAGATTGCAC TATGACTCTA CTGAACTCAA ACTCTTTGAG
    AATATTGAAT GTGAATGGCC TTTGTTCTGG ACATATTTAC TTATTGATGG AATTTTTAAT
    GGGGATCAGG TTCAGATGCA AGAATATCGG GAAGCTCTGG AGGGAATTCT CATAAGAGGA
    AAGAATGGAA TTCGTCTTGT GCCAGAACTT TATGCTGTTC CTACTGACAA GATAGAAGAA
    GAGTACCATA ACCCGCATAC AGTTGACCGC AGACCTTTGG GGAAGATACC TCACCTATGG
    GGACAATCAC TGTACATACT GGGCTCTCTT TTAGCTGAGG GATTTCTAGC ACCAGGAGAA
    GTAGATCCGT TGAACAGAAG ATTCTCTACA GTTTTTAAGC CTGATGTTGT GGTGCAAGTT
    TCAGTTCTAG CAGAGTCTAA AGAGATAAAG AAAATGCTCA AAAACCATGG CCTTGAAGTT
    CAGAGCTTTA GTGATGTCCG GCCAATCAGA GTGCAGCCAG CTAGGATTTT GAGCCACCTG
    TATGCCAAGC TTGGGCATAA TAAAGCCATG AAGCTTAGTG GCCGCCCATA CCGTCACATA
    GGTGTTCTAG GGACGTCTAA ACTGTACATG ATCAGGAACC AGATCTTTAC TTTTACACCC
    CAGTTTACAG ATCAGCACCA CTTCTATCTA GCCCTCGACA GCAGAATGAT TGTGGAGATG
    CTTAGAACTG AACTGGCCTA TTTAACTTCC TGCTGGAGGA TGACTGGGAG ACCAACGCTA
    ACCTTTCCCA TTACCCATTC TATGCTCAAT GAAGATGGCT CTGATATTGA TCCTGCTATT
    CTTTCTACAA TTAGAAAGAT GGAAGATGGA TATTTTGGTG GCGCAAGGGT GAAGCTTGGA
    AAGTTATCAG ATTTTCTTAC TACATCTTTC TACACTCATC TAAGTTTTCT GGATTCTGAC
    TGTGATGGAA AGCTTTTTGA TGATGTAGAT GACGAATGTT ATAGTCCCGT CAATGACTAT
    GAAGTGGAGT TATTTTCGAA TACAGAGTGT GAGAAAGGTA ATATAACTAA AGAGGAAGTT
    CAGGATGAGC TTGATCAGTA TATAAATCAA CTGCAGCAAA GCATGGCTTC AACATCTTAC
    CTCCCTCCTA TCTCCAGTCA CTCCGGCACA TACCATGCCT TCAGCACACT CCATTCCACC
    CGGGATATTC TATCGATAAT GGCTAAATCT AAAGGCCTGG ATGTACCAAA TTCCTCCCTA
    TTTCTTCCTG CTAAAAATCT GAGTGCTCAT CGGAGATCTT TAAATCTGTT AGAGGTCCCA
    CAACTACACA AACACCAGAA TCATGAAACT GAATTAAATT TACCAAAAGA TGCCCATGGG
    GATATTGACT GCCACAAGCT GCTGGATAAA CTCAAGGAAT GCCCAACTCT TCAAGACCAA
    GCCGATATTC TTTATATACT GTGTGTGATA AAAGGACTGG ACTGGGATAC AGGGCTGAAT
    GGACAGTGTG GTGTGTCGGT ACATTTGCTG CTAAATGAGT TATATAGCAA GGCAGGACTC
    AATCGGGAAT GGGGTTTGAT CCGTTACATC TCTGGCCTTC TGAAAAAGAG AGTGGAGGTC
    CTTGCTGAGG CTTGTACTGA CATCTTGTCG CACCATAAGC AGCTTACTGT TGGCCTTCCC
    CCGGAACCCC GAGAGAAAAC CATCACAGCA CCCCTTCCTC CGGAACAGCT GGCAGCCCTG
    ATTTATGAAG CGAGTGGACA AGATATTAGC ATTGCTGTAC TTACACAGGA AATAATGCTT
    TATCTGGCTA TGTATGTCAG ATCTCAGCCA AGTCTCTTTG GAGAGATGCT GAGGCTGCGG
    ATTGGACTCA TAATGCAGGT GATGGCAACT GAACTTGCAC GCAGCCTAAA CTGCTCTGGT
    GAAGAAGCTT CAGAAAGTTT GATGAGCCTC AGCCCTTTTG ATATGAAGAA CCTACTTCAC
    CATATCCTGA GTGGTAAAGA GTTTGGCGTA GAACGAAGCA TGCGTCCAAT TGAATCATCG
    GCCACTAGCC CCGCTATATC TATCCACGAA ATGGGACACA CAGGAGCAAC GAAAACAGAA
    AGGAGCGGCA TCAACAAGCT CAGGAATGAA ATGAAACAAA TGGATAGGAG GTTTAGCCAA
    GATGATCAGT TTGGGCATTC TGTCGCCAGC AGCATCCATT CCTCCAGGTC TACGAGATGC
    AGCAGCCCTT CTTCTCCCAC GGGTACTCTC TCCCCAACGG GATTTGTTGG ACCCCATCTT
    GTTTTCGATG ACCACCAAGG TCAGTGGTTG AGAAGGAGAA GGCTTGATGG GGCTATAAAC
    CGCGTACCGG TCGGATTCTA CCAGAGAGTA TGGAATGTTT TGCAAAAGTG CCATGGTTTA
    TCAATTGATG GGTATGTTCT TCCTTCCTCG AGCACACGAG AGATGACTGC TTGTGAGATC
    AAGTTTGCTG TCCATGTGGA ATCTGTTCTG AATCATGTTC CCCAGCCTGA ATACAGGCAG
    CTGCTAGTGG AAGCTATAAA TGTGCTCACC TTGGTGTCAG ACATGGAAGT GGATAGCTTT
    GGTAGCATTA TCCACGTCGA CCGCATTGTG CACTCTGCCA ATGACCTCTT CCTTCAGGAC
    CAGAAAGCCC TCGGTGCTGC AGATTTCTAC CTTGACCTGG ATCAAGCTAC GGGAATCTGC
    AACTTCTTCT ATGACAGCGC TCCAAGTGGA GCCTATGGCA CAATGACTTA CCTAACAAAA
    GCAGTAGCCA TCAGCTTACA GGATTTCTTA CCAAATACCA GCTGTTCAAT GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946976.1
    CDS181..3357
    Translation

    Target ORF information:

    RefSeq Version XM_018091487.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGGAGC GAGGAGACAA AACAAATCAA GGAATTCCTG AGCTAAATGC AAGCTCTGTG 
    GGCATGGCAA AGGCTGCTCT GGAAGCTATA GATGAACTTG ATCTGTTTGG CGCTCATGGG
    GGACCCAAAT CTGTGATTCA TGTTCTACCT GATGATGTAG AACACTGTCA GTCAATCCTT
    TATTCAATGT TGCCTCGGGC CTCCACCTCC AAAGAAATTG ATGCTGGCCT CCTCTCCATC
    ATTTCATATC CTGCTTTTGC TGTTGAGGAC ATAAATCTGG TTAATGTGAC CAAAAATGAA
    ATAATCACTA AACTCCAGGG AAGATATGGC TGCTGTCGCT TTCTCAGAGA TGGCTTTAAA
    ACACCAAGAG AGGACCCCAC CAGATTGCAC TATGACTCTA CTGAACTCAA ACTCTTTGAG
    AATATTGAAT GTGAATGGCC TTTGTTCTGG ACATATTTAC TTATTGATGG AATTTTTAAT
    GGGGATCAGG TTCAGATGCA AGAATATCGG GAAGCTCTGG AGGGAATTCT CATAAGAGGA
    AAGAATGGAA TTCGTCTTGT GCCAGAACTT TATGCTGTTC CTACTGACAA GATAGAAGAA
    GAGTACCATA ACCCGCATAC AGTTGACCGC AGACCTTTGG GGAAGATACC TCACCTATGG
    GGACAATCAC TGTACATACT GGGCTCTCTT TTAGCTGAGG GATTTCTAGC ACCAGGAGAA
    GTAGATCCGT TGAACAGAAG ATTCTCTACA GTTTTTAAGC CTGATGTTGT GGTGCAAGTT
    TCAGTTCTAG CAGAGTCTAA AGAGATAAAG AAAATGCTCA AAAACCATGG CCTTGAAGTT
    CAGAGCTTTA GTGATGTCCG GCCAATCAGA GTGCAGCCAG CTAGGATTTT GAGCCACCTG
    TATGCCAAGC TTGGGCATAA TAAAGCCATG AAGCTTAGTG GCCGCCCATA CCGTCACATA
    GGTGTTCTAG GGACGTCTAA ACTGTACATG ATCAGGAACC AGATCTTTAC TTTTACACCC
    CAGTTTACAG ATCAGCACCA CTTCTATCTA GCCCTCGACA GCAGAATGAT TGTGGAGATG
    CTTAGAACTG AACTGGCCTA TTTAACTTCC TGCTGGAGGA TGACTGGGAG ACCAACGCTA
    ACCTTTCCCA TTACCCATTC TATGCTCAAT GAAGATGGCT CTGATATTGA TCCTGCTATT
    CTTTCTACAA TTAGAAAGAT GGAAGATGGA TATTTTGGTG GCGCAAGGGT GAAGCTTGGA
    AAGTTATCAG ATTTTCTTAC TACATCTTTC TACACTCATC TAAGTTTTCT GGATTCTGAC
    TGTGATGGAA AGCTTTTTGA TGATGTAGAT GACGAATGTT ATAGTCCCGT CAATGACTAT
    GAAGTGGAGT TATTTTCGAA TACAGAGTGT GAGAAAGGTA ATATAACTAA AGAGGAAGTT
    CAGGATGAGC TTGATCAGTA TATAAATCAA CTGCAGCAAA GCATGGCTTC AACATCTTAC
    CTCCCTCCTA TCTCCAGTCA CTCCGGCACA TACCATGCCT TCAGCACACT CCATTCCACC
    CGGGATATTC TATCGATAAT GGCTAAATCT AAAGGCCTGG ATGTACCAAA TTCCTCCCTA
    TTTCTTCCTG CTAAAAATCT GAGTGCTCAT CGGAGATCTT TAAATCTGTT AGAGGTCCCA
    CAACTACACA AACACCAGAA TCATGAAACT GAATTAAATT TACCAAAAGA TGCCCATGGG
    GATATTGACT GCCACAAGCT GCTGGATAAA CTCAAGGAAT GCCCAACTCT TCAAGACCAA
    GCCGATATTC TTTATATACT GTGTGTGATA AAAGGACTGG ACTGGGATAC AGGGCTGAAT
    GGACAGTGTG GTGTGTCGGT ACATTTGCTG CTAAATGAGT TATATAGCAA GGCAGGACTC
    AATCGGGAAT GGGGTTTGAT CCGTTACATC TCTGGCCTTC TGAAAAAGAG AGTGGAGGTC
    CTTGCTGAGG CTTGTACTGA CATCTTGTCG CACCATAAGC AGCTTACTGT TGGCCTTCCC
    CCGGAACCCC GAGAGAAAAC CATCACAGCA CCCCTTCCTC CGGAACAGCT GGCAGCCCTG
    ATTTATGAAG CGAGTGGACA AGATATTAGC ATTGCTGTAC TTACACAGGA AATAATGCTT
    TATCTGGCTA TGTATGTCAG ATCTCAGCCA AGTCTCTTTG GAGAGATGCT GAGGCTGCGG
    ATTGGACTCA TAATGCAGGT GATGGCAACT GAACTTGCAC GCAGCCTAAA CTGCTCTGGT
    GAAGAAGCTT CAGAAAGTTT GATGAGCCTC AGCCCTTTTG ATATGAAGAA CCTACTTCAC
    CATATCCTGA GTGGTAAAGA GTTTGGCGTA GAACGAAGCA TGCGTCCAAT TGAATCATCG
    GCCACTAGCC CCGCTATATC TATCCACGAA ATGGGACACA CAGGAGCAAC GAAAACAGAA
    AGGAGCGGCA TCAACAAGCT CAGGAATGAA ATGAAACAAA TGGATAGGAG GTTTAGCCAA
    GATGATCAGT TTGGGCATTC TGTCGCCAGC AGCATCCATT CCTCCAGGTC TACGAGATGC
    AGCAGCCCTT CTTCTCCCAC GGGTACTCTC TCCCCAACGG GATTTGTTGG ACCCCATCTT
    GTTTTCGATG ACCACCAAGG TCAGTGGTTG AGAAGGAGAA GGCTTGATGG GGCTATAAAC
    CGCGTACCGG TCGGATTCTA CCAGAGAGTA TGGAATGTTT TGCAAAAGTG CCATGGTTTA
    TCAATTGATG GGTATGTTCT TCCTTCCTCG AGCACACGAG AGATGACTGC TTGTGAGATC
    AAGTTTGCTG TCCATGTGGA ATCTGTTCTG AATCATGTTC CCCAGCCTGA ATACAGGCAG
    CTGCTAGTGG AAGCTATAAA TGTGCTCACC TTGGTGTCAG ACATGGAAGT GGATAGCTTT
    GGTAGCATTA TCCACGTCGA CCGCATTGTG CACTCTGCCA ATGACCTCTT CCTTCAGGAC
    CAGAAAGCCC TCGGTGCTGC AGATTTCTAC CTTGACCTGG ATCAAGCTAC GGGAATCTGC
    AACTTCTTCT ATGACAGCGC TCCAAGTGGA GCCTATGGCA CAATGACTTA CCTAACAAAA
    GCAGTAGCCA TCAGCTTACA GGATTTCTTA CCAAATACCA GCTGTTCAAT GCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52143
    Clone ID Related Accession (Same CDS sequence) XM_012957495.3 , XM_012957496.3
    Accession Version XM_012957495.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCGC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812949.2
    CDS476..4198
    Translation

    Target ORF information:

    RefSeq Version XM_012957495.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957495.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCGC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52143
    Clone ID Related Accession (Same CDS sequence) XM_012957495.3 , XM_012957496.3
    Accession Version XM_012957496.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCGC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812950.2
    CDS416..4138
    Translation

    Target ORF information:

    RefSeq Version XM_012957496.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957496.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCGC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52144
    Clone ID Related Accession (Same CDS sequence) XM_004911663.4
    Accession Version XM_004911663.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911663.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGAGGAAGT TCAGGATGAG
    CTTGATCAGT ATATAAATCA ACTGCAGCAA AGCATGGCTT CAACATCTTA CCTCCCTCCT
    ATCTCCAGTC ACTCCGGCAC ATACCATGCC TTCAGCACAC TCCATTCCAC CCGGGATATT
    CTATCGATAA TGGCTAAATC TAAAGGCCTG GATGTACCAA ATTCCTCCCT ATTTCTTCCT
    GCTAAAAATC TGAGTGCTCA TCGGAGATCT TTAAATCTGT TAGAGGTCCC ACAACTACAC
    AAACACCAGA ATCATGAAAC TGAATTAAAT TTACCAAAAG ATGCCCATGG GGATATTGAC
    TGCCACAAGC TGCTGGATAA ACTCAAGGAA TGCCCAACTC TTCAAGACCA AGCCGATATT
    CTTTATATAC TGTGTGTGAT AAAAGGACTG GACTGGGATA CAGGGCTGAA TGGACAGTGT
    GGTGTGTCGG TACATTTGCT GCTAAATGAG TTATATAGCA AGGCAGGACT CAATCGGGAA
    TGGGGTTTGA TCCGTTACAT CTCTGGCCTT CTGAAAAAGA GAGTGGAGGT CCTTGCTGAG
    GCTTGTACTG ACATCTTGTC GCACCATAAG CAGCTTACTG TTGGCCTTCC CCCGGAACCC
    CGAGAGAAAA CCATCACAGC ACCCCTTCCT CCGGAACAGC TGGCAGCCCT GATTTATGAA
    GCGAGTGGAC AAGATATTAG CATTGCTGTA CTTACACAGG AAATAATGCT TTATCTGGCT
    ATGTATGTCA GATCTCAGCC AAGTCTCTTT GGAGAGATGC TGAGGCTGCG GATTGGACTC
    ATAATGCAGG TGATGGCAAC TGAACTTGCA CGCAGCCTAA ACTGCTCTGG TGAAGAAGCT
    TCAGAAAGTT TGATGAGCCT CAGCCCTTTT GATATGAAGA ACCTACTTCA CCATATCCTG
    AGTGGTAAAG AGTTTGGCGT AGAACGAAGC ATGCGTCCAA TTGAATCATC GGCCACTAGC
    CCCGCTATAT CTATCCACGA AATGGGACAC ACAGGAGCAA CGAAAACAGA AAGGAGCGGC
    ATCAACAAGC TCAGGAATGA AATGAAACAA ATGGATAGGA GGTTTAGCCA AGATGATCAG
    TTTGGGCATT CTGTCGCCAG CAGCATCCAT TCCGCCAGGT CTACGAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911720.2
    CDS476..4183
    Translation

    Target ORF information:

    RefSeq Version XM_004911663.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911663.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGAGGAAGT TCAGGATGAG
    CTTGATCAGT ATATAAATCA ACTGCAGCAA AGCATGGCTT CAACATCTTA CCTCCCTCCT
    ATCTCCAGTC ACTCCGGCAC ATACCATGCC TTCAGCACAC TCCATTCCAC CCGGGATATT
    CTATCGATAA TGGCTAAATC TAAAGGCCTG GATGTACCAA ATTCCTCCCT ATTTCTTCCT
    GCTAAAAATC TGAGTGCTCA TCGGAGATCT TTAAATCTGT TAGAGGTCCC ACAACTACAC
    AAACACCAGA ATCATGAAAC TGAATTAAAT TTACCAAAAG ATGCCCATGG GGATATTGAC
    TGCCACAAGC TGCTGGATAA ACTCAAGGAA TGCCCAACTC TTCAAGACCA AGCCGATATT
    CTTTATATAC TGTGTGTGAT AAAAGGACTG GACTGGGATA CAGGGCTGAA TGGACAGTGT
    GGTGTGTCGG TACATTTGCT GCTAAATGAG TTATATAGCA AGGCAGGACT CAATCGGGAA
    TGGGGTTTGA TCCGTTACAT CTCTGGCCTT CTGAAAAAGA GAGTGGAGGT CCTTGCTGAG
    GCTTGTACTG ACATCTTGTC GCACCATAAG CAGCTTACTG TTGGCCTTCC CCCGGAACCC
    CGAGAGAAAA CCATCACAGC ACCCCTTCCT CCGGAACAGC TGGCAGCCCT GATTTATGAA
    GCGAGTGGAC AAGATATTAG CATTGCTGTA CTTACACAGG AAATAATGCT TTATCTGGCT
    ATGTATGTCA GATCTCAGCC AAGTCTCTTT GGAGAGATGC TGAGGCTGCG GATTGGACTC
    ATAATGCAGG TGATGGCAAC TGAACTTGCA CGCAGCCTAA ACTGCTCTGG TGAAGAAGCT
    TCAGAAAGTT TGATGAGCCT CAGCCCTTTT GATATGAAGA ACCTACTTCA CCATATCCTG
    AGTGGTAAAG AGTTTGGCGT AGAACGAAGC ATGCGTCCAA TTGAATCATC GGCCACTAGC
    CCCGCTATAT CTATCCACGA AATGGGACAC ACAGGAGCAA CGAAAACAGA AAGGAGCGGC
    ATCAACAAGC TCAGGAATGA AATGAAACAA ATGGATAGGA GGTTTAGCCA AGATGATCAG
    TTTGGGCATT CTGTCGCCAG CAGCATCCAT TCCGCCAGGT CTACGAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52145
    Clone ID Related Accession (Same CDS sequence) XM_031896750.1
    Accession Version XM_031896750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGAGATG CAGCAGCCCT TCTTCTCCCA CGGGTACTCT CTCCCCAACG
    GGATTTGTTG GACCCCATCT TGTTTTCGAT GACCACCAAG GTCAGTGGTT GAGAAGGAGA
    AGGCTTGATG GGGCTATAAA CCGCGTACCG GTCGGATTCT ACCAGAGAGT ATGGAATGTT
    TTGCAAAAGT GCCATGGTTT ATCAATTGAT GGGTATGTTC TTCCTTCCTC GAGCACACGA
    GAGATGACTG CTTGTGAGAT CAAGTTTGCT GTCCATGTGG AATCTGTTCT GAATCATGTT
    CCCCAGCCTG AATACAGGCA GCTGCTAGTG GAAGCTATAA ATGTGCTCAC CTTGGTGTCA
    GACATGGAAG TGGATAGCTT TGGTAGCATT ATCCACGTCG ACCGCATTGT GCACTCTGCC
    AATGACCTCT TCCTTCAGGA CCAGAAAGCC CTCGGTGCTG CAGATTTCTA CCTTGACCTG
    GATCAAGCTA CGGGAATCTG CAACTTCTTC TATGACAGCG CTCCAAGTGG AGCCTATGGC
    ACAATGACTT ACCTAACAAA AGCAGTAGCC ATCAGCTTAC AGGATTTCTT ACCAAATACC
    AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752610.1
    CDS476..4153
    Translation

    Target ORF information:

    RefSeq Version XM_031896750.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031896750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGAGATG CAGCAGCCCT TCTTCTCCCA CGGGTACTCT CTCCCCAACG
    GGATTTGTTG GACCCCATCT TGTTTTCGAT GACCACCAAG GTCAGTGGTT GAGAAGGAGA
    AGGCTTGATG GGGCTATAAA CCGCGTACCG GTCGGATTCT ACCAGAGAGT ATGGAATGTT
    TTGCAAAAGT GCCATGGTTT ATCAATTGAT GGGTATGTTC TTCCTTCCTC GAGCACACGA
    GAGATGACTG CTTGTGAGAT CAAGTTTGCT GTCCATGTGG AATCTGTTCT GAATCATGTT
    CCCCAGCCTG AATACAGGCA GCTGCTAGTG GAAGCTATAA ATGTGCTCAC CTTGGTGTCA
    GACATGGAAG TGGATAGCTT TGGTAGCATT ATCCACGTCG ACCGCATTGT GCACTCTGCC
    AATGACCTCT TCCTTCAGGA CCAGAAAGCC CTCGGTGCTG CAGATTTCTA CCTTGACCTG
    GATCAAGCTA CGGGAATCTG CAACTTCTTC TATGACAGCG CTCCAAGTGG AGCCTATGGC
    ACAATGACTT ACCTAACAAA AGCAGTAGCC ATCAGCTTAC AGGATTTCTT ACCAAATACC
    AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05606
    Clone ID Related Accession (Same CDS sequence) XM_004911663.3
    Accession Version XM_004911663.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGAGGAAGT TCAGGATGAG
    CTTGATCAGT ATATAAATCA ACTGCAGCAA AGCATGGCTT CAACATCTTA CCTCCCTCCT
    ATCTCCAGTC ACTCCGGCAC ATACCATGCC TTCAGCACAC TCCATTCCAC CCGGGATATT
    CTATCGATAA TGGCTAAATC TAAAGGCCTG GATGTACCAA ATTCCTCCCT ATTTCTTCCT
    GCTAAAAATC TGAGTGCTCA TCGGAGATCT TTAAATCTGT TAGAGGTCCC ACAACTACAC
    AAACACCAGA ATCATGAAAC TGAATTAAAT TTACCAAAAG ATGCCCATGG GGATATTGAC
    TGCCACAAGC TGCTGGATAA ACTCAAGGAA TGCCCAACTC TTCAAGACCA AGCCGATATT
    CTTTATATAC TGTGTGTGAT AAAAGGACTG GACTGGGATA CAGGGCTGAA TGGACAGTGT
    GGTGTGTCGG TACATTTGCT GCTAAATGAG TTATATAGCA AGGCAGGACT CAATCGGGAA
    TGGGGTTTGA TCCGTTACAT CTCTGGCCTT CTGAAAAAGA GAGTGGAGGT CCTTGCTGAG
    GCTTGTACTG ACATCTTGTC GCACCATAAG CAGCTTACTG TTGGCCTTCC CCCGGAACCC
    CGAGAGAAAA CCATCACAGC ACCCCTTCCT CCGGAACAGC TGGCAGCCCT GATTTATGAA
    GCGAGTGGAC AAGATATTAG CATTGCTGTA CTTACACAGG AAATAATGCT TTATCTGGCT
    ATGTATGTCA GATCTCAGCC AAGTCTCTTT GGAGAGATGC TGAGGCTGCG GATTGGACTC
    ATAATGCAGG TGATGGCAAC TGAACTTGCA CGCAGCCTAA ACTGCTCTGG TGAAGAAGCT
    TCAGAAAGTT TGATGAGCCT CAGCCCTTTT GATATGAAGA ACCTACTTCA CCATATCCTG
    AGTGGTAAAG AGTTTGGCGT AGAACGAAGC ATGCGTCCAA TTGAATCATC GGCCACTAGC
    CCCGCTATAT CTATCCACGA AATGGGACAC ACAGGAGCAA CGAAAACAGA AAGGAGCGGC
    ATCAACAAGC TCAGGAATGA AATGAAACAA ATGGATAGGA GGTTTAGCCA AGATGATCAG
    TTTGGGCATT CTGTCGCCAG CAGCATCCAT TCCTCCAGGT CTACGAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911720.1
    CDS499..4206
    Translation

    Target ORF information:

    RefSeq Version XM_004911663.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGAGGAAGT TCAGGATGAG
    CTTGATCAGT ATATAAATCA ACTGCAGCAA AGCATGGCTT CAACATCTTA CCTCCCTCCT
    ATCTCCAGTC ACTCCGGCAC ATACCATGCC TTCAGCACAC TCCATTCCAC CCGGGATATT
    CTATCGATAA TGGCTAAATC TAAAGGCCTG GATGTACCAA ATTCCTCCCT ATTTCTTCCT
    GCTAAAAATC TGAGTGCTCA TCGGAGATCT TTAAATCTGT TAGAGGTCCC ACAACTACAC
    AAACACCAGA ATCATGAAAC TGAATTAAAT TTACCAAAAG ATGCCCATGG GGATATTGAC
    TGCCACAAGC TGCTGGATAA ACTCAAGGAA TGCCCAACTC TTCAAGACCA AGCCGATATT
    CTTTATATAC TGTGTGTGAT AAAAGGACTG GACTGGGATA CAGGGCTGAA TGGACAGTGT
    GGTGTGTCGG TACATTTGCT GCTAAATGAG TTATATAGCA AGGCAGGACT CAATCGGGAA
    TGGGGTTTGA TCCGTTACAT CTCTGGCCTT CTGAAAAAGA GAGTGGAGGT CCTTGCTGAG
    GCTTGTACTG ACATCTTGTC GCACCATAAG CAGCTTACTG TTGGCCTTCC CCCGGAACCC
    CGAGAGAAAA CCATCACAGC ACCCCTTCCT CCGGAACAGC TGGCAGCCCT GATTTATGAA
    GCGAGTGGAC AAGATATTAG CATTGCTGTA CTTACACAGG AAATAATGCT TTATCTGGCT
    ATGTATGTCA GATCTCAGCC AAGTCTCTTT GGAGAGATGC TGAGGCTGCG GATTGGACTC
    ATAATGCAGG TGATGGCAAC TGAACTTGCA CGCAGCCTAA ACTGCTCTGG TGAAGAAGCT
    TCAGAAAGTT TGATGAGCCT CAGCCCTTTT GATATGAAGA ACCTACTTCA CCATATCCTG
    AGTGGTAAAG AGTTTGGCGT AGAACGAAGC ATGCGTCCAA TTGAATCATC GGCCACTAGC
    CCCGCTATAT CTATCCACGA AATGGGACAC ACAGGAGCAA CGAAAACAGA AAGGAGCGGC
    ATCAACAAGC TCAGGAATGA AATGAAACAA ATGGATAGGA GGTTTAGCCA AGATGATCAG
    TTTGGGCATT CTGTCGCCAG CAGCATCCAT TCCTCCAGGT CTACGAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37158
    Clone ID Related Accession (Same CDS sequence) XM_012957495.2 , XM_012957496.2
    Accession Version XM_012957495.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCTC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812949.1
    CDS499..4221
    Translation

    Target ORF information:

    RefSeq Version XM_012957495.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCTC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37158
    Clone ID Related Accession (Same CDS sequence) XM_012957495.2 , XM_012957496.2
    Accession Version XM_012957496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCTC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812950.1
    CDS439..4161
    Translation

    Target ORF information:

    RefSeq Version XM_012957496.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAATGGA TAGGAGGTTT
    AGCCAAGATG ATCAGTTTGG GCATTCTGTC GCCAGCAGCA TCCATTCCTC CAGGTCTACG
    AGATGCAGCA GCCCTTCTTC TCCCACGGGT ACTCTCTCCC CAACGGGATT TGTTGGACCC
    CATCTTGTTT TCGATGACCA CCAAGGTCAG TGGTTGAGAA GGAGAAGGCT TGATGGGGCT
    ATAAACCGCG TACCGGTCGG ATTCTACCAG AGAGTATGGA ATGTTTTGCA AAAGTGCCAT
    GGTTTATCAA TTGATGGGTA TGTTCTTCCT TCCTCGAGCA CACGAGAGAT GACTGCTTGT
    GAGATCAAGT TTGCTGTCCA TGTGGAATCT GTTCTGAATC ATGTTCCCCA GCCTGAATAC
    AGGCAGCTGC TAGTGGAAGC TATAAATGTG CTCACCTTGG TGTCAGACAT GGAAGTGGAT
    AGCTTTGGTA GCATTATCCA CGTCGACCGC ATTGTGCACT CTGCCAATGA CCTCTTCCTT
    CAGGACCAGA AAGCCCTCGG TGCTGCAGAT TTCTACCTTG ACCTGGATCA AGCTACGGGA
    ATCTGCAACT TCTTCTATGA CAGCGCTCCA AGTGGAGCCT ATGGCACAAT GACTTACCTA
    ACAAAAGCAG TAGCCATCAG CTTACAGGAT TTCTTACCAA ATACCAGCTG TTCAATGCAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37159
    Clone ID Related Accession (Same CDS sequence) XM_012957497.2 , XM_012957497.3
    Accession Version XM_012957497.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812951.1
    CDS500..4147
    Translation

    Target ORF information:

    RefSeq Version XM_012957497.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGATA
    GAAGAAGAGT ACCATAACCC GCATACAGTT GACCGCAGAC CTTTGGGGAA GATACCTCAC
    CTATGGGGAC AATCACTGTA CATACTGGGC TCTCTTTTAG CTGAGGGATT TCTAGCACCA
    GGAGAAGTAG ATCCGTTGAA CAGAAGATTC TCTACAGTTT TTAAGCCTGA TGTTGTGGTG
    CAAGTTTCAG TTCTAGCAGA GTCTAAAGAG ATAAAGAAAA TGCTCAAAAA CCATGGCCTT
    GAAGTTCAGA GCTTTAGTGA TGTCCGGCCA ATCAGAGTGC AGCCAGCTAG GATTTTGAGC
    CACCTGTATG CCAAGCTTGG GCATAATAAA GCCATGAAGC TTAGTGGCCG CCCATACCGT
    CACATAGGTG TTCTAGGGAC GTCTAAACTG TACATGATCA GGAACCAGAT CTTTACTTTT
    ACACCCCAGT TTACAGATCA GCACCACTTC TATCTAGCCC TCGACAGCAG AATGATTGTG
    GAGATGCTTA GAACTGAACT GGCCTATTTA ACTTCCTGCT GGAGGATGAC TGGGAGACCA
    ACGCTAACCT TTCCCATTAC CCATTCTATG CTCAATGAAG ATGGCTCTGA TATTGATCCT
    GCTATTCTTT CTACAATTAG AAAGATGGAA GATGGATATT TTGGTGGCGC AAGGGTGAAG
    CTTGGAAAGT TATCAGATTT TCTTACTACA TCTTTCTACA CTCATCTAAG TTTTCTGGAT
    TCTGACTGTG ATGGAAAGCT TTTTGATGAT GTAGATGACG AATGTTATAG TCCCGTCAAT
    GACTATGAAG TGGAGTTATT TTCGAATACA GAGTGTGAGA AAGGTAATAT AACTAAAGAG
    GAAGTTCAGG ATGAGCTTGA TCAGTATATA AATCAACTGC AGCAAAGCAT GGCTTCAACA
    TCTTACCTCC CTCCTATCTC CAGTCACTCC GGCACATACC ATGCCTTCAG CACACTCCAT
    TCCACCCGGG ATATTCTATC GATAATGGCT AAATCTAAAG GCCTGGATGT ACCAAATTCC
    TCCCTATTTC TTCCTGCTAA AAATCTGAGT GCTCATCGGA GATCTTTAAA TCTGTTAGAG
    GTCCCACAAC TACACAAACA CCAGAATCAT GAAACTGAAT TAAATTTACC AAAAGATGCC
    CATGGGGATA TTGACTGCCA CAAGCTGCTG GATAAACTCA AGGAATGCCC AACTCTTCAA
    GACCAAGCCG ATATTCTTTA TATACTGTGT GTGATAAAAG GACTGGACTG GGATACAGGG
    CTGAATGGAC AGTGTGGTGT GTCGGTACAT TTGCTGCTAA ATGAGTTATA TAGCAAGGCA
    GGACTCAATC GGGAATGGGG TTTGATCCGT TACATCTCTG GCCTTCTGAA AAAGAGAGTG
    GAGGTCCTTG CTGAGGCTTG TACTGACATC TTGTCGCACC ATAAGCAGCT TACTGTTGGC
    CTTCCCCCGG AACCCCGAGA GAAAACCATC ACAGCACCCC TTCCTCCGGA ACAGCTGGCA
    GCCCTGATTT ATGAAGCGAG TGGACAAGAT ATTAGCATTG CTGTACTTAC ACAGGAAATA
    ATGCTTTATC TGGCTATGTA TGTCAGATCT CAGCCAAGTC TCTTTGGAGA GATGCTGAGG
    CTGCGGATTG GACTCATAAT GCAGGTGATG GCAACTGAAC TTGCACGCAG CCTAAACTGC
    TCTGGTGAAG AAGCTTCAGA AAGTTTGATG AGCCTCAGCC CTTTTGATAT GAAGAACCTA
    CTTCACCATA TCCTGAGTGG TAAAGAGTTT GGCGTAGAAC GAAGCATGCG TCCAATTGAA
    TCATCGGCCA CTAGCCCCGC TATATCTATC CACGAAATGG GACACACAGG AGCAACGAAA
    ACAGAAAGGA GCGGCATCAA CAAGCTCAGG AATGAAATGA AACAAAGATG CAGCAGCCCT
    TCTTCTCCCA CGGGTACTCT CTCCCCAACG GGATTTGTTG GACCCCATCT TGTTTTCGAT
    GACCACCAAG GTCAGTGGTT GAGAAGGAGA AGGCTTGATG GGGCTATAAA CCGCGTACCG
    GTCGGATTCT ACCAGAGAGT ATGGAATGTT TTGCAAAAGT GCCATGGTTT ATCAATTGAT
    GGGTATGTTC TTCCTTCCTC GAGCACACGA GAGATGACTG CTTGTGAGAT CAAGTTTGCT
    GTCCATGTGG AATCTGTTCT GAATCATGTT CCCCAGCCTG AATACAGGCA GCTGCTAGTG
    GAAGCTATAA ATGTGCTCAC CTTGGTGTCA GACATGGAAG TGGATAGCTT TGGTAGCATT
    ATCCACGTCG ACCGCATTGT GCACTCTGCC AATGACCTCT TCCTTCAGGA CCAGAAAGCC
    CTCGGTGCTG CAGATTTCTA CCTTGACCTG GATCAAGCTA CGGGAATCTG CAACTTCTTC
    TATGACAGCG CTCCAAGTGG AGCCTATGGC ACAATGACTT ACCTAACAAA AGCAGTAGCC
    ATCAGCTTAC AGGATTTCTT ACCAAATACC AGCTGTTCAA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37160
    Clone ID Related Accession (Same CDS sequence) XM_012957498.2
    Accession Version XM_012957498.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGGGA
    TTTCTAGCAC CAGGAGAAGT AGATCCGTTG AACAGAAGAT TCTCTACAGT TTTTAAGCCT
    GATGTTGTGG TGCAAGTTTC AGTTCTAGCA GAGTCTAAAG AGATAAAGAA AATGCTCAAA
    AACCATGGCC TTGAAGTTCA GAGCTTTAGT GATGTCCGGC CAATCAGAGT GCAGCCAGCT
    AGGATTTTGA GCCACCTGTA TGCCAAGCTT GGGCATAATA AAGCCATGAA GCTTAGTGGC
    CGCCCATACC GTCACATAGG TGTTCTAGGG ACGTCTAAAC TGTACATGAT CAGGAACCAG
    ATCTTTACTT TTACACCCCA GTTTACAGAT CAGCACCACT TCTATCTAGC CCTCGACAGC
    AGAATGATTG TGGAGATGCT TAGAACTGAA CTGGCCTATT TAACTTCCTG CTGGAGGATG
    ACTGGGAGAC CAACGCTAAC CTTTCCCATT ACCCATTCTA TGCTCAATGA AGATGGCTCT
    GATATTGATC CTGCTATTCT TTCTACAATT AGAAAGATGG AAGATGGATA TTTTGGTGGC
    GCAAGGGTGA AGCTTGGAAA GTTATCAGAT TTTCTTACTA CATCTTTCTA CACTCATCTA
    AGTTTTCTGG ATTCTGACTG TGATGGAAAG CTTTTTGATG ATGTAGATGA CGAATGTTAT
    AGTCCCGTCA ATGACTATGA AGTGGAGTTA TTTTCGAATA CAGAGTGTGA GAAAGGTAAT
    ATAACTAAAG AGGAAGTTCA GGATGAGCTT GATCAGTATA TAAATCAACT GCAGCAAAGC
    ATGGCTTCAA CATCTTACCT CCCTCCTATC TCCAGTCACT CCGGCACATA CCATGCCTTC
    AGCACACTCC ATTCCACCCG GGATATTCTA TCGATAATGG CTAAATCTAA AGGCCTGGAT
    GTACCAAATT CCTCCCTATT TCTTCCTGCT AAAAATCTGA GTGCTCATCG GAGATCTTTA
    AATCTGTTAG AGGTCCCACA ACTACACAAA CACCAGAATC ATGAAACTGA ATTAAATTTA
    CCAAAAGATG CCCATGGGGA TATTGACTGC CACAAGCTGC TGGATAAACT CAAGGAATGC
    CCAACTCTTC AAGACCAAGC CGATATTCTT TATATACTGT GTGTGATAAA AGGACTGGAC
    TGGGATACAG GGCTGAATGG ACAGTGTGGT GTGTCGGTAC ATTTGCTGCT AAATGAGTTA
    TATAGCAAGG CAGGACTCAA TCGGGAATGG GGTTTGATCC GTTACATCTC TGGCCTTCTG
    AAAAAGAGAG TGGAGGTCCT TGCTGAGGCT TGTACTGACA TCTTGTCGCA CCATAAGCAG
    CTTACTGTTG GCCTTCCCCC GGAACCCCGA GAGAAAACCA TCACAGCACC CCTTCCTCCG
    GAACAGCTGG CAGCCCTGAT TTATGAAGCG AGTGGACAAG ATATTAGCAT TGCTGTACTT
    ACACAGGAAA TAATGCTTTA TCTGGCTATG TATGTCAGAT CTCAGCCAAG TCTCTTTGGA
    GAGATGCTGA GGCTGCGGAT TGGACTCATA ATGCAGGTGA TGGCAACTGA ACTTGCACGC
    AGCCTAAACT GCTCTGGTGA AGAAGCTTCA GAAAGTTTGA TGAGCCTCAG CCCTTTTGAT
    ATGAAGAACC TACTTCACCA TATCCTGAGT GGTAAAGAGT TTGGCGTAGA ACGAAGCATG
    CGTCCAATTG AATCATCGGC CACTAGCCCC GCTATATCTA TCCACGAAAT GGGACACACA
    GGAGCAACGA AAACAGAAAG GAGCGGCATC AACAAGCTCA GGAATGAAAT GAAACAAATG
    GATAGGAGGT TTAGCCAAGA TGATCAGTTT GGGCATTCTG TCGCCAGCAG CATCCATTCC
    TCCAGGTCTA CGAGATGCAG CAGCCCTTCT TCTCCCACGG GTACTCTCTC CCCAACGGGA
    TTTGTTGGAC CCCATCTTGT TTTCGATGAC CACCAAGGTC AGTGGTTGAG AAGGAGAAGG
    CTTGATGGGG CTATAAACCG CGTACCGGTC GGATTCTACC AGAGAGTATG GAATGTTTTG
    CAAAAGTGCC ATGGTTTATC AATTGATGGG TATGTTCTTC CTTCCTCGAG CACACGAGAG
    ATGACTGCTT GTGAGATCAA GTTTGCTGTC CATGTGGAAT CTGTTCTGAA TCATGTTCCC
    CAGCCTGAAT ACAGGCAGCT GCTAGTGGAA GCTATAAATG TGCTCACCTT GGTGTCAGAC
    ATGGAAGTGG ATAGCTTTGG TAGCATTATC CACGTCGACC GCATTGTGCA CTCTGCCAAT
    GACCTCTTCC TTCAGGACCA GAAAGCCCTC GGTGCTGCAG ATTTCTACCT TGACCTGGAT
    CAAGCTACGG GAATCTGCAA CTTCTTCTAT GACAGCGCTC CAAGTGGAGC CTATGGCACA
    ATGACTTACC TAACAAAAGC AGTAGCCATC AGCTTACAGG ATTTCTTACC AAATACCAGC
    TGTTCAATGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812952.1
    CDS499..4113
    Translation

    Target ORF information:

    RefSeq Version XM_012957498.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phosphorylase kinase regulatory subunit alpha 2 (phka2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957498.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAGCA GGAGTAATTC AGGGGTCAGA CTTGATGGCT ACGCCAGGCT TATTCAGCAG 
    ACCATACTTT GTCACCAGAA CCCAGTCACA GGCCTTCTTC CTGCTAGCAA AGAGCAAAAA
    GATGCCTGGG TGCGAGACAA TGTGTACAGT ATTCTGGCTG TGTGGGGACT GGGAATGGCG
    TATCGCAAAA ATGCTGACCG GGATGAGGAC AAAGCCAAAG CATATGAACT AGAGCAGAGT
    GTTGTAAAGC TGATGAGAGG AATCTTGCAC TGCATGATAA GACAGGTGGA TAAAGTTGAG
    AAATTCAAAC ACACGCAGAG TACAAAAGAT TGTCTGCATG CCAAGTACAA CACGGCAACG
    TGCAGTACTA TAGTTGGTGA TGACCAGTGG GGACACCTCC AAGTAGATGC TACCTCCCTA
    TTCCTGTTGT GCTTGGCACA AATGACTGCT GCAGGCTTAC GCATTGTGTT TACACTGAAT
    GAAGTTGCCT TTATACAGAA TCTTGTTTTT TACATTGAAG CTGCTTATAA AGTTGCTGAT
    TATGGAATGT GGGAGCGAGG AGACAAAACA AATCAAGGAA TTCCTGAGCT AAATGCAAGC
    TCTGTGGGCA TGGCAAAGGC TGCTCTGGAA GCTATAGATG AACTTGATCT GTTTGGCGCT
    CATGGGGGAC CCAAATCTGT GATTCATGTT CTACCTGATG ATGTAGAACA CTGTCAGTCA
    ATCCTTTATT CAATGTTGCC TCGGGCCTCC ACCTCCAAAG AAATTGATGC TGGCCTCCTC
    TCCATCATTT CATATCCTGC TTTTGCTGTT GAGGACATAA ATCTGGTTAA TGTGACCAAA
    AATGAAATAA TCACTAAACT CCAGGGAAGA TATGGCTGCT GTCGCTTTCT CAGAGATGGC
    TTTAAAACAC CAAGAGAGGA CCCCACCAGA TTGCACTATG ACTCTACTGA ACTCAAACTC
    TTTGAGAATA TTGAATGTGA ATGGCCTTTG TTCTGGACAT ATTTACTTAT TGATGGAATT
    TTTAATGGGG ATCAGGTTCA GATGCAAGAA TATCGGGAAG CTCTGGAGGG AATTCTCATA
    AGAGGAAAGA ATGGAATTCG TCTTGTGCCA GAACTTTATG CTGTTCCTAC TGACAAGGGA
    TTTCTAGCAC CAGGAGAAGT AGATCCGTTG AACAGAAGAT TCTCTACAGT TTTTAAGCCT
    GATGTTGTGG TGCAAGTTTC AGTTCTAGCA GAGTCTAAAG AGATAAAGAA AATGCTCAAA
    AACCATGGCC TTGAAGTTCA GAGCTTTAGT GATGTCCGGC CAATCAGAGT GCAGCCAGCT
    AGGATTTTGA GCCACCTGTA TGCCAAGCTT GGGCATAATA AAGCCATGAA GCTTAGTGGC
    CGCCCATACC GTCACATAGG TGTTCTAGGG ACGTCTAAAC TGTACATGAT CAGGAACCAG
    ATCTTTACTT TTACACCCCA GTTTACAGAT CAGCACCACT TCTATCTAGC CCTCGACAGC
    AGAATGATTG TGGAGATGCT TAGAACTGAA CTGGCCTATT TAACTTCCTG CTGGAGGATG
    ACTGGGAGAC CAACGCTAAC CTTTCCCATT ACCCATTCTA TGCTCAATGA AGATGGCTCT
    GATATTGATC CTGCTATTCT TTCTACAATT AGAAAGATGG AAGATGGATA TTTTGGTGGC
    GCAAGGGTGA AGCTTGGAAA GTTATCAGAT TTTCTTACTA CATCTTTCTA CACTCATCTA
    AGTTTTCTGG ATTCTGACTG TGATGGAAAG CTTTTTGATG ATGTAGATGA CGAATGTTAT
    AGTCCCGTCA ATGACTATGA AGTGGAGTTA TTTTCGAATA CAGAGTGTGA GAAAGGTAAT
    ATAACTAAAG AGGAAGTTCA GGATGAGCTT GATCAGTATA TAAATCAACT GCAGCAAAGC
    ATGGCTTCAA CATCTTACCT CCCTCCTATC TCCAGTCACT CCGGCACATA CCATGCCTTC
    AGCACACTCC ATTCCACCCG GGATATTCTA TCGATAATGG CTAAATCTAA AGGCCTGGAT
    GTACCAAATT CCTCCCTATT TCTTCCTGCT AAAAATCTGA GTGCTCATCG GAGATCTTTA
    AATCTGTTAG AGGTCCCACA ACTACACAAA CACCAGAATC ATGAAACTGA ATTAAATTTA
    CCAAAAGATG CCCATGGGGA TATTGACTGC CACAAGCTGC TGGATAAACT CAAGGAATGC
    CCAACTCTTC AAGACCAAGC CGATATTCTT TATATACTGT GTGTGATAAA AGGACTGGAC
    TGGGATACAG GGCTGAATGG ACAGTGTGGT GTGTCGGTAC ATTTGCTGCT AAATGAGTTA
    TATAGCAAGG CAGGACTCAA TCGGGAATGG GGTTTGATCC GTTACATCTC TGGCCTTCTG
    AAAAAGAGAG TGGAGGTCCT TGCTGAGGCT TGTACTGACA TCTTGTCGCA CCATAAGCAG
    CTTACTGTTG GCCTTCCCCC GGAACCCCGA GAGAAAACCA TCACAGCACC CCTTCCTCCG
    GAACAGCTGG CAGCCCTGAT TTATGAAGCG AGTGGACAAG ATATTAGCAT TGCTGTACTT
    ACACAGGAAA TAATGCTTTA TCTGGCTATG TATGTCAGAT CTCAGCCAAG TCTCTTTGGA
    GAGATGCTGA GGCTGCGGAT TGGACTCATA ATGCAGGTGA TGGCAACTGA ACTTGCACGC
    AGCCTAAACT GCTCTGGTGA AGAAGCTTCA GAAAGTTTGA TGAGCCTCAG CCCTTTTGAT
    ATGAAGAACC TACTTCACCA TATCCTGAGT GGTAAAGAGT TTGGCGTAGA ACGAAGCATG
    CGTCCAATTG AATCATCGGC CACTAGCCCC GCTATATCTA TCCACGAAAT GGGACACACA
    GGAGCAACGA AAACAGAAAG GAGCGGCATC AACAAGCTCA GGAATGAAAT GAAACAAATG
    GATAGGAGGT TTAGCCAAGA TGATCAGTTT GGGCATTCTG TCGCCAGCAG CATCCATTCC
    TCCAGGTCTA CGAGATGCAG CAGCCCTTCT TCTCCCACGG GTACTCTCTC CCCAACGGGA
    TTTGTTGGAC CCCATCTTGT TTTCGATGAC CACCAAGGTC AGTGGTTGAG AAGGAGAAGG
    CTTGATGGGG CTATAAACCG CGTACCGGTC GGATTCTACC AGAGAGTATG GAATGTTTTG
    CAAAAGTGCC ATGGTTTATC AATTGATGGG TATGTTCTTC CTTCCTCGAG CACACGAGAG
    ATGACTGCTT GTGAGATCAA GTTTGCTGTC CATGTGGAAT CTGTTCTGAA TCATGTTCCC
    CAGCCTGAAT ACAGGCAGCT GCTAGTGGAA GCTATAAATG TGCTCACCTT GGTGTCAGAC
    ATGGAAGTGG ATAGCTTTGG TAGCATTATC CACGTCGACC GCATTGTGCA CTCTGCCAAT
    GACCTCTTCC TTCAGGACCA GAAAGCCCTC GGTGCTGCAG ATTTCTACCT TGACCTGGAT
    CAAGCTACGG GAATCTGCAA CTTCTTCTAT GACAGCGCTC CAAGTGGAGC CTATGGCACA
    ATGACTTACC TAACAAAAGC AGTAGCCATC AGCTTACAGG ATTTCTTACC AAATACCAGC
    TGTTCAATGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.