mcf2l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mcf2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcf2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa06192 XM_002933006.5
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa52505 XM_012957900.3
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52505 XM_012957901.3
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52506 XM_031897030.1
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52507 XM_012957903.3
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52508 XM_012957904.3
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52509 XM_031897031.1
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa52510 XM_031897033.1
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa52510 XM_031897032.1
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa52511 XM_012957906.3
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa36977 XM_012957900.2
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa36977 XM_012957901.2
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa36979 XM_012957903.2
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa36980 XM_012957904.2
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa36982 XM_012957906.2
Latest version!
Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OXa06192 XM_002933006.4 Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa06192
    Clone ID Related Accession (Same CDS sequence) XM_002933006.5 , XM_002933006.4
    Accession Version XM_002933006.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933006.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA
    CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT
    AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT
    GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT
    GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG
    AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC
    AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA
    TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933052.2
    CDS513..3995
    Translation

    Target ORF information:

    RefSeq Version XM_002933006.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933006.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA
    CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT
    AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT
    GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT
    GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG
    AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC
    AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA
    TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52505
    Clone ID Related Accession (Same CDS sequence) XM_012957901.3 , XM_012957900.3
    Accession Version XM_012957901.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813355.1
    CDS36..3686
    Translation

    Target ORF information:

    RefSeq Version XM_012957901.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957901.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52505
    Clone ID Related Accession (Same CDS sequence) XM_012957901.3 , XM_012957900.3
    Accession Version XM_012957900.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813354.1
    CDS514..4164
    Translation

    Target ORF information:

    RefSeq Version XM_012957900.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957900.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52506
    Clone ID Related Accession (Same CDS sequence) XM_031897030.1
    Accession Version XM_031897030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGC AAAGAAGAGA GAGGCACGAG GGCCAGCACA ATGGTAGGTT CTCCTCACGA 
    GTGACTCATG TACTTCACAG TGCGTCAGCC TCGGTTCTCC GCTCCCTACC CAGGAGAGGA
    TGGAAGAAAA GCGCCCATGC CGCTTATTTT GATGACATCA TGCAACAGGA GACCTTTCCA
    ATCTGTGCAG CCGATATTGA GGATCAGCTA AGAAAGCAGT TTGCCTACTT GTCAGGTGGA
    AGAGGGGAAA ATGGCTGTCC AGTCGTCACA TTCCCTGAAT ACCCAGCATT TAGCGAGATT
    CCAGAAAAAG AGTTCCAGAA TGTACTCACC TATCTGACAA GTGTCCCAAT TTTACAAGAT
    GCGGGAATTG GCTTCATCTT AGTGATAGAC AGAAGGCTGG ACAAATGGAC CTCAGTGAAA
    GCCTCTATCC TGAGGATTGC GGCATCTTTT CCTGGGAATT TACAGCTTGT TATAGTCCTG
    CGCCCATCAG GACTATTGCA GCGCACCCTC TCGGATATTG CATTCAAATT CAACAAAGAT
    GACTTTAAAA TGAAAGTACC GATCATAATG CTGAATTCAG CAACAGAACT CCATAGTTAT
    ATTGACAAGA CCCAGCTGAC TCGAGATCTT GGTGGCACTC TGGATTACTG CCATACTAAC
    TGGCTCTCAC ATCGCACTGC TATTGAAAAC TTTGCTGTCA TGGTGAAACA AACAGCCCAA
    ATGCTCCAGT CTTTTGGGAC AGAGCTGGCA GAGACAGAGC TGCCAAATGA TGTTCCCTCT
    ACAAGCTTGC TGCTCCACGC ACACACAGAG AAAAAGGAAA AAATGAAGGA TCACCTTAAG
    ATAGCACTGA CACAGGGATG TGACATTTTG AACAAGATCA TGGAGCCACG TAAAGAGAAC
    CCTGAGTATA GGATGAATCA GGACCAACTG GATAACCAAA CTACTGTGGA AAGGCTTCTT
    TTGCAATTAA ATGAAACAGA AGCTGCTTTT GATGAGTTTT GGTCTAAACA TGAACAGAAG
    CTTGAACAGT GTTTACAGCT GCGTCATTTT GAGTTAAACT TCAGAGAGAT TTCTACAAGT
    CTGGATGGTA TTTCTGAGAG GCTAGAAGCT TTTACAGATG TTGGAAACAG CTGCTCCCAT
    GCTGAACACA TCCTGAAGGA CTTCTGTAAC TTTGAAGTTA AATCCCGAGA AGTTTTACAG
    ACAGCCCAGA ATTTAGTCAG AGAAGGTGAT CAACTCATTA AAAGTAATCA CTATGCTGTG
    GACTCCATTC TTCCAAAATG CGACGAGATT GGTCACTTAT GTGACAGCAT TAGCAACACT
    ATTCAGCAGA GACGGCAAAA CCTTCACAAA TCCTTGGAAC TTCACAGACA TTTGGAGAAG
    ACAATGAAGT GGTGTGATGA AGGGATTTTC TTGCTGGCGT CCCAACCTGT GGATAAGTTC
    CAGTCTCAGG ATGGGGCCGA AGCTGCACTT CAGGAGATTA CAAGGTTTCT AGAGACTGGA
    ACAGATATAA CCATGAAAGA TTTGGCTTTC ATTTTGAAGA TGTACAACTC GATTCTCACT
    CCAGAACTCC AGGAACATGT CAATAAGGTA TACCAGAAGC AGGAAAGCAT GCAGGAGATG
    TTTGAGAAAA GGAAAGGTAG CCTTAGAAAA CTTGCAGCAA AGCAAACCAG ACCTGTCCAG
    CCTGTTGCAC CTAGACCAGA AGCAATTTCC AAGTCTCCTA CCTCCTCTCC AGGTCTTCAT
    AGGTCTTCTG AAAGCAGATA TAGCTCAGGA AGTATTCAAC GACGGATAAA TTATCGCAGA
    GCCAAGAGCG AAGTGGATGG TAGTCAGATG AGAAGTACAT CAGTGATGGA AGACGAAGAA
    AATCTAGCAG TGTTAAGAAG ACATGTCATG AATGAACTTA TTGAGACAGA AAGGGCATAT
    GTGGAAGAAC TTCTATCTGT GATTGAGGGT TATGCTGCTG AAATGAATAA TCCTCTAGTG
    ATGGACCTTA TCCCCTCAGT GCTCCAGAAT AAGAAGGATG TTCTTTTCGG CAACATGGAA
    GAAATTTACC AGTTTCATAA CAGAATATTT CTGAGAGAGC TGGAACATTA TAGAGACTAT
    CCAGAACTAG TGGGAAGGTG TTTTTTGGAA AGGATGGAAG ATTTCCAGAT CTATGAAAAG
    TACTGCCAGA ACAAGCCACG GTCAGAGAGT TTGTGGAGGC AATGCTCCGA TGCTGCTTTC
    TTCCAGGATT GCCAAAAGAA ACTGGACCAT AAACTCAGCC TGGATTCCTA TCTTCTAAAG
    CCGGTGCAAA GGATAACAAA GTATCAGCTT CTGTTAAAGG AAATGCTTAA GTATAGTAAA
    AACTGTGAAG GAGCTGAAGA TCTTCAGGAA GCACTTGCCT CTATATTGGG CATTCTTAAA
    GCTGTCAATG ATTCCATGCA TCAGATAGCA ATCACTGGAT ATGAGGGGAA CTTACATGAA
    CTTGGTAAAC TCTTAATGCA AGGATCATTT AATGTATGGA CAGAGCACAA GAAGGGCCAT
    TCCAAAGTAA AGGACCTTGC TAGGTTTAAA CCAATGCAAA GACACCTGTT TCTGCATGAA
    AAAGCTGTGC TGTTCTGCAA GAAGAGGGAT GAGAATGGGG AGGGCTATGA GAAGGCACCA
    TCCTACAGCT ACAAACATTC CTTGAATATG ATGTTTGTTG GAATTACTGA GTATGTAAAA
    GGAGACAATA GGAAATTTGA AATTTGGTAC AACGCAAGGG AGGAGGTTTA TATTGTGCAG
    GCACCAACCA CAGAAGTAAA GGCCACCTGG GTAAATGAAA TCAGAAAGGT GCTGACAAGC
    CAGTTAAAGG CACACAGAGA TGCCAGCCAC CATAAATCGG CAGAGCACGC AAACAGCTTA
    CCTACTCCTC TCCCATCCAG TACTAGTCCC CTGAGAAACA AATCTGGGAA GATCAAAAAG
    CTGGAGGAAA GAAAAACTGA GCCTTTATTG AATCCGGATG TCTCCTTAAC ATCTGTACCA
    AAATACCTTG AAAAAGGCAA AGATGGCACA GTAACTAGTC CTACCTCAGA CTCTTCTGCC
    TTGTCAAAAA AGCGGTTCAC TTTGCAGGGT TTTTCAAACC TCAAAAGTCA GAAAGCCTCT
    CCTACCAGCC CAGACAAAAA AGCTAAACGC CATGAAGTAA AGAGTGACCC AACTCCCTTT
    GGTTTAAGAG GTTGGAACAG AGCATCACAT TCACTTGATG TCTCTGAAGA AAATAATGAA
    GATTGGTCTA GCGGAGAGGA TCCAATTAAC TCTTCAGATG CAGAAGAAGA TGGAGGAGGT
    GATGCACCAG AGAAAAAGCT AACTCCTGGT AAATATACTG TTGTGGCTGA TTACGAGAGA
    GGTTCAGATG AGCTGCTGGT AAAGAGTGGA GATCTTGTTC ACATAATACA TGAAGAGGAT
    GGTGGAATGT GGTATGTGAA GAACCTGAGT ACCAATAAGG AAGGTCTGGT ACCTGCTAGC
    AACCTAATGA CTCTCATAGG CACTTCCAAA TCTGCCCTCT CTTTAAGCAG CTCTGAATCG
    GGACCCGCTT CAAGCACTTT AAGCACATCT TCTAGCTGTA GTGAGAGCTG CAATGCAGGC
    ACCACCAGTT TCTCTGATAT CAAGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752890.1
    CDS605..4234
    Translation

    Target ORF information:

    RefSeq Version XM_031897030.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTCAGC AAAGAAGAGA GAGGCACGAG GGCCAGCACA ATGGTAGGTT CTCCTCACGA 
    GTGACTCATG TACTTCACAG TGCGTCAGCC TCGGTTCTCC GCTCCCTACC CAGGAGAGGA
    TGGAAGAAAA GCGCCCATGC CGCTTATTTT GATGACATCA TGCAACAGGA GACCTTTCCA
    ATCTGTGCAG CCGATATTGA GGATCAGCTA AGAAAGCAGT TTGCCTACTT GTCAGGTGGA
    AGAGGGGAAA ATGGCTGTCC AGTCGTCACA TTCCCTGAAT ACCCAGCATT TAGCGAGATT
    CCAGAAAAAG AGTTCCAGAA TGTACTCACC TATCTGACAA GTGTCCCAAT TTTACAAGAT
    GCGGGAATTG GCTTCATCTT AGTGATAGAC AGAAGGCTGG ACAAATGGAC CTCAGTGAAA
    GCCTCTATCC TGAGGATTGC GGCATCTTTT CCTGGGAATT TACAGCTTGT TATAGTCCTG
    CGCCCATCAG GACTATTGCA GCGCACCCTC TCGGATATTG CATTCAAATT CAACAAAGAT
    GACTTTAAAA TGAAAGTACC GATCATAATG CTGAATTCAG CAACAGAACT CCATAGTTAT
    ATTGACAAGA CCCAGCTGAC TCGAGATCTT GGTGGCACTC TGGATTACTG CCATACTAAC
    TGGCTCTCAC ATCGCACTGC TATTGAAAAC TTTGCTGTCA TGGTGAAACA AACAGCCCAA
    ATGCTCCAGT CTTTTGGGAC AGAGCTGGCA GAGACAGAGC TGCCAAATGA TGTTCCCTCT
    ACAAGCTTGC TGCTCCACGC ACACACAGAG AAAAAGGAAA AAATGAAGGA TCACCTTAAG
    ATAGCACTGA CACAGGGATG TGACATTTTG AACAAGATCA TGGAGCCACG TAAAGAGAAC
    CCTGAGTATA GGATGAATCA GGACCAACTG GATAACCAAA CTACTGTGGA AAGGCTTCTT
    TTGCAATTAA ATGAAACAGA AGCTGCTTTT GATGAGTTTT GGTCTAAACA TGAACAGAAG
    CTTGAACAGT GTTTACAGCT GCGTCATTTT GAGTTAAACT TCAGAGAGAT TTCTACAAGT
    CTGGATGGTA TTTCTGAGAG GCTAGAAGCT TTTACAGATG TTGGAAACAG CTGCTCCCAT
    GCTGAACACA TCCTGAAGGA CTTCTGTAAC TTTGAAGTTA AATCCCGAGA AGTTTTACAG
    ACAGCCCAGA ATTTAGTCAG AGAAGGTGAT CAACTCATTA AAAGTAATCA CTATGCTGTG
    GACTCCATTC TTCCAAAATG CGACGAGATT GGTCACTTAT GTGACAGCAT TAGCAACACT
    ATTCAGCAGA GACGGCAAAA CCTTCACAAA TCCTTGGAAC TTCACAGACA TTTGGAGAAG
    ACAATGAAGT GGTGTGATGA AGGGATTTTC TTGCTGGCGT CCCAACCTGT GGATAAGTTC
    CAGTCTCAGG ATGGGGCCGA AGCTGCACTT CAGGAGATTA CAAGGTTTCT AGAGACTGGA
    ACAGATATAA CCATGAAAGA TTTGGCTTTC ATTTTGAAGA TGTACAACTC GATTCTCACT
    CCAGAACTCC AGGAACATGT CAATAAGGTA TACCAGAAGC AGGAAAGCAT GCAGGAGATG
    TTTGAGAAAA GGAAAGGTAG CCTTAGAAAA CTTGCAGCAA AGCAAACCAG ACCTGTCCAG
    CCTGTTGCAC CTAGACCAGA AGCAATTTCC AAGTCTCCTA CCTCCTCTCC AGGTCTTCAT
    AGGTCTTCTG AAAGCAGATA TAGCTCAGGA AGTATTCAAC GACGGATAAA TTATCGCAGA
    GCCAAGAGCG AAGTGGATGG TAGTCAGATG AGAAGTACAT CAGTGATGGA AGACGAAGAA
    AATCTAGCAG TGTTAAGAAG ACATGTCATG AATGAACTTA TTGAGACAGA AAGGGCATAT
    GTGGAAGAAC TTCTATCTGT GATTGAGGGT TATGCTGCTG AAATGAATAA TCCTCTAGTG
    ATGGACCTTA TCCCCTCAGT GCTCCAGAAT AAGAAGGATG TTCTTTTCGG CAACATGGAA
    GAAATTTACC AGTTTCATAA CAGAATATTT CTGAGAGAGC TGGAACATTA TAGAGACTAT
    CCAGAACTAG TGGGAAGGTG TTTTTTGGAA AGGATGGAAG ATTTCCAGAT CTATGAAAAG
    TACTGCCAGA ACAAGCCACG GTCAGAGAGT TTGTGGAGGC AATGCTCCGA TGCTGCTTTC
    TTCCAGGATT GCCAAAAGAA ACTGGACCAT AAACTCAGCC TGGATTCCTA TCTTCTAAAG
    CCGGTGCAAA GGATAACAAA GTATCAGCTT CTGTTAAAGG AAATGCTTAA GTATAGTAAA
    AACTGTGAAG GAGCTGAAGA TCTTCAGGAA GCACTTGCCT CTATATTGGG CATTCTTAAA
    GCTGTCAATG ATTCCATGCA TCAGATAGCA ATCACTGGAT ATGAGGGGAA CTTACATGAA
    CTTGGTAAAC TCTTAATGCA AGGATCATTT AATGTATGGA CAGAGCACAA GAAGGGCCAT
    TCCAAAGTAA AGGACCTTGC TAGGTTTAAA CCAATGCAAA GACACCTGTT TCTGCATGAA
    AAAGCTGTGC TGTTCTGCAA GAAGAGGGAT GAGAATGGGG AGGGCTATGA GAAGGCACCA
    TCCTACAGCT ACAAACATTC CTTGAATATG ATGTTTGTTG GAATTACTGA GTATGTAAAA
    GGAGACAATA GGAAATTTGA AATTTGGTAC AACGCAAGGG AGGAGGTTTA TATTGTGCAG
    GCACCAACCA CAGAAGTAAA GGCCACCTGG GTAAATGAAA TCAGAAAGGT GCTGACAAGC
    CAGTTAAAGG CACACAGAGA TGCCAGCCAC CATAAATCGG CAGAGCACGC AAACAGCTTA
    CCTACTCCTC TCCCATCCAG TACTAGTCCC CTGAGAAACA AATCTGGGAA GATCAAAAAG
    CTGGAGGAAA GAAAAACTGA GCCTTTATTG AATCCGGATG TCTCCTTAAC ATCTGTACCA
    AAATACCTTG AAAAAGGCAA AGATGGCACA GTAACTAGTC CTACCTCAGA CTCTTCTGCC
    TTGTCAAAAA AGCGGTTCAC TTTGCAGGGT TTTTCAAACC TCAAAAGTCA GAAAGCCTCT
    CCTACCAGCC CAGACAAAAA AGCTAAACGC CATGAAGTAA AGAGTGACCC AACTCCCTTT
    GGTTTAAGAG GTTGGAACAG AGCATCACAT TCACTTGATG TCTCTGAAGA AAATAATGAA
    GATTGGTCTA GCGGAGAGGA TCCAATTAAC TCTTCAGATG CAGAAGAAGA TGGAGGAGGT
    GATGCACCAG AGAAAAAGCT AACTCCTGGT AAATATACTG TTGTGGCTGA TTACGAGAGA
    GGTTCAGATG AGCTGCTGGT AAAGAGTGGA GATCTTGTTC ACATAATACA TGAAGAGGAT
    GGTGGAATGT GGTATGTGAA GAACCTGAGT ACCAATAAGG AAGGTCTGGT ACCTGCTAGC
    AACCTAATGA CTCTCATAGG CACTTCCAAA TCTGCCCTCT CTTTAAGCAG CTCTGAATCG
    GGACCCGCTT CAAGCACTTT AAGCACATCT TCTAGCTGTA GTGAGAGCTG CAATGCAGGC
    ACCACCAGTT TCTCTGATAT CAAGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52507
    Clone ID Related Accession (Same CDS sequence) XM_012957903.3
    Accession Version XM_012957903.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
    TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC
    TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA
    GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA
    GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG
    GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC
    TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC
    CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC
    TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT
    GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG
    CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG
    CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA
    AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA
    GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT
    GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG
    CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT
    GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG
    GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT
    GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA
    GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC
    TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT
    CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA
    ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG
    TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA
    GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA
    GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT
    GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT
    GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG
    TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC
    AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT
    CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG
    GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG
    GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA
    ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA
    GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC
    TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC
    CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG
    GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC
    TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT
    GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT
    GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC
    AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA
    GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC
    TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA
    GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA
    CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG
    TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT
    ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG
    GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA
    TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG
    TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCCTCTCCT
    ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT
    TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT
    TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT
    GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT
    TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT
    GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC
    CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA
    CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC
    ACCAGTTTCT CTGATATCAA GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813357.1
    CDS149..3715
    Translation

    Target ORF information:

    RefSeq Version XM_012957903.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957903.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
    TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC
    TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA
    GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA
    GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG
    GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC
    TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC
    CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC
    TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT
    GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG
    CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG
    CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA
    AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA
    GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT
    GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG
    CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT
    GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG
    GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT
    GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA
    GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC
    TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT
    CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA
    ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG
    TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA
    GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA
    GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT
    GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT
    GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG
    TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC
    AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT
    CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG
    GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG
    GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA
    ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA
    GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC
    TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC
    CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG
    GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC
    TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT
    GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT
    GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC
    AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA
    GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC
    TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA
    GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA
    CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG
    TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT
    ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG
    GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA
    TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG
    TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCCTCTCCT
    ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT
    TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT
    TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT
    GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT
    TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT
    GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC
    CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA
    CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC
    ACCAGTTTCT CTGATATCAA GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52508
    Clone ID Related Accession (Same CDS sequence) XM_012957904.3
    Accession Version XM_012957904.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCCTCTCC TACCAGCCCA
    GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT
    TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC
    GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG
    AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG
    CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG
    TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT
    CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA
    AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC
    TCTGATATCA AGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813358.1
    CDS513..4070
    Translation

    Target ORF information:

    RefSeq Version XM_012957904.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957904.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCCTCTCC TACCAGCCCA
    GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT
    TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC
    GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG
    AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG
    CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG
    TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT
    CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA
    AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC
    TCTGATATCA AGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52509
    Clone ID Related Accession (Same CDS sequence) XM_031897031.1
    Accession Version XM_031897031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGTGG AAGAACTGCT ACGGAGATTA AATGAGGTTT TACACAACAC TGATGACATC 
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752891.1
    CDS49..3579
    Translation

    Target ORF information:

    RefSeq Version XM_031897031.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTGTGG AAGAACTGCT ACGGAGATTA AATGAGGTTT TACACAACAC TGATGACATC 
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52510
    Clone ID Related Accession (Same CDS sequence) XM_031897032.1 , XM_031897033.1
    Accession Version XM_031897032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752892.1
    CDS310..3780
    Translation

    Target ORF information:

    RefSeq Version XM_031897032.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52510
    Clone ID Related Accession (Same CDS sequence) XM_031897032.1 , XM_031897033.1
    Accession Version XM_031897033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031752893.1
    CDS225..3695
    Translation

    Target ORF information:

    RefSeq Version XM_031897033.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG 
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52511
    Clone ID Related Accession (Same CDS sequence) XM_012957906.3
    Accession Version XM_012957906.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813360.1
    CDS514..3966
    Translation

    Target ORF information:

    RefSeq Version XM_012957906.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957906.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCCTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa06192
    Clone ID Related Accession (Same CDS sequence) XM_002933006.5 , XM_002933006.4
    Accession Version XM_002933006.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA
    CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT
    AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT
    GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT
    GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG
    AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC
    AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA
    TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002933052.2
    CDS672..4154
    Translation

    Target ORF information:

    RefSeq Version XM_002933006.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002933006.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGGTTGGAA CAGAGCATCA
    CATTCACTTG ATGTCTCTGA AGAAAATAAT GAAGATTGGT CTAGCGGAGA GGATCCAATT
    AACTCTTCAG ATGCAGAAGA AGATGGAGGA GGTGATGCAC CAGAGAAAAA GCTAACTCCT
    GGTAAATATA CTGTTGTGGC TGATTACGAG AGAGGTTCAG ATGAGCTGCT GGTAAAGAGT
    GGAGATCTTG TTCACATAAT ACATGAAGAG GATGGTGGAA TGTGGTATGT GAAGAACCTG
    AGTACCAATA AGGAAGGTCT GGTACCTGCT AGCAACCTAA TGACTCTCAT AGGCACTTCC
    AAATCTGCCC TCTCTTTAAG CAGCTCTGAA TCGGGACCCG CTTCAAGCAC TTTAAGCACA
    TCTTCTAGCT GTAGTGAGAG CTGCAATGCA GGCACCACCA GTTTCTCTGA TATCAAGGGT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36977
    Clone ID Related Accession (Same CDS sequence) XM_012957900.2 , XM_012957901.2
    Accession Version XM_012957900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813354.1
    CDS678..4328
    Translation

    Target ORF information:

    RefSeq Version XM_012957900.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36977
    Clone ID Related Accession (Same CDS sequence) XM_012957900.2 , XM_012957901.2
    Accession Version XM_012957901.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813355.1
    CDS28..3678
    Translation

    Target ORF information:

    RefSeq Version XM_012957901.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957901.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGGTATGTGA AGAACCTGAG TACCAATAAG
    GAAGGTCTGG TACCTGCTAG CAACCTAATG ACTCTCATAG GCACTTCCAA ATCTGCCCTC
    TCTTTAAGCA GCTCTGAATC GGGACCCGCT TCAAGCACTT TAAGCACATC TTCTAGCTGT
    AGTGAGAGCT GCAATGCAGG CACCACCAGT TTCTCTGATA TCAAGGGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36979
    Clone ID Related Accession (Same CDS sequence) XM_012957903.2
    Accession Version XM_012957903.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957903.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
    TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC
    TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA
    GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA
    GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG
    GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC
    TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC
    CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC
    TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT
    GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG
    CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG
    CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA
    AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA
    GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT
    GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG
    CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT
    GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG
    GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT
    GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA
    GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC
    TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT
    CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA
    ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG
    TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA
    GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA
    GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT
    GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT
    GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG
    TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC
    AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT
    CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG
    GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG
    GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA
    ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA
    GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC
    TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC
    CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG
    GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC
    TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT
    GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT
    GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC
    AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA
    GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC
    TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA
    GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA
    CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG
    TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT
    ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG
    GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA
    TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG
    TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCTTCTCCT
    ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT
    TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT
    TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT
    GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT
    TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT
    GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC
    CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA
    CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC
    ACCAGTTTCT CTGATATCAA GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813357.1
    CDS195..3761
    Translation

    Target ORF information:

    RefSeq Version XM_012957903.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957903.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTGTG TAAAAATTTC AGTGGAAGAA ATGAAAGTCT ACCATTGGTT TTCTGAGATG 
    TGCTTAATGC TCCATTCTTC GGTGCACAAT GACATCATGC AACAGGAGAC CTTTCCAATC
    TGTGCAGCCG ATATTGAGGA TCAGCTAAGA AAGCAGTTTG CCTACTTGTC AGGTGGAAGA
    GGGGAAAATG GCTGTCCAGT CGTCACATTC CCTGAATACC CAGCATTTAG CGAGATTCCA
    GAAAAAGAGT TCCAGAATGT ACTCACCTAT CTGACAAGTG TCCCAATTTT ACAAGATGCG
    GGAATTGGCT TCATCTTAGT GATAGACAGA AGGCTGGACA AATGGACCTC AGTGAAAGCC
    TCTATCCTGA GGATTGCGGC ATCTTTTCCT GGGAATTTAC AGCTTGTTAT AGTCCTGCGC
    CCATCAGGAC TATTGCAGCG CACCCTCTCG GATATTGCAT TCAAATTCAA CAAAGATGAC
    TTTAAAATGA AAGTACCGAT CATAATGCTG AATTCAGCAA CAGAACTCCA TAGTTATATT
    GACAAGACCC AGCTGACTCG AGATCTTGGT GGCACTCTGG ATTACTGCCA TACTAACTGG
    CTCTCACATC GCACTGCTAT TGAAAACTTT GCTGTCATGG TGAAACAAAC AGCCCAAATG
    CTCCAGTCTT TTGGGACAGA GCTGGCAGAG ACAGAGCTGC CAAATGATGT TCCCTCTACA
    AGCTTGCTGC TCCACGCACA CACAGAGAAA AAGGAAAAAA TGAAGGATCA CCTTAAGATA
    GCACTGACAC AGGGATGTGA CATTTTGAAC AAGATCATGG AGCCACGTAA AGAGAACCCT
    GAGTATAGGA TGAATCAGGA CCAACTGGAT AACCAAACTA CTGTGGAAAG GCTTCTTTTG
    CAATTAAATG AAACAGAAGC TGCTTTTGAT GAGTTTTGGT CTAAACATGA ACAGAAGCTT
    GAACAGTGTT TACAGCTGCG TCATTTTGAG TTAAACTTCA GAGAGATTTC TACAAGTCTG
    GATGGTATTT CTGAGAGGCT AGAAGCTTTT ACAGATGTTG GAAACAGCTG CTCCCATGCT
    GAACACATCC TGAAGGACTT CTGTAACTTT GAAGTTAAAT CCCGAGAAGT TTTACAGACA
    GCCCAGAATT TAGTCAGAGA AGGTGATCAA CTCATTAAAA GTAATCACTA TGCTGTGGAC
    TCCATTCTTC CAAAATGCGA CGAGATTGGT CACTTATGTG ACAGCATTAG CAACACTATT
    CAGCAGAGAC GGCAAAACCT TCACAAATCC TTGGAACTTC ACAGACATTT GGAGAAGACA
    ATGAAGTGGT GTGATGAAGG GATTTTCTTG CTGGCGTCCC AACCTGTGGA TAAGTTCCAG
    TCTCAGGATG GGGCCGAAGC TGCACTTCAG GAGATTACAA GGTTTCTAGA GACTGGAACA
    GATATAACCA TGAAAGATTT GGCTTTCATT TTGAAGATGT ACAACTCGAT TCTCACTCCA
    GAACTCCAGG AACATGTCAA TAAGGTATAC CAGAAGCAGG AAAGCATGCA GGAGATGTTT
    GAGAAAAGGA AAGGTAGCCT TAGAAAACTT GCAGCAAAGC AAACCAGACC TGTCCAGCCT
    GTTGCACCTA GACCAGAAGC AATTTCCAAG TCTCCTACCT CCTCTCCAGG TCTTCATAGG
    TCTTCTGAAA GCAGATATAG CTCAGGAAGT ATTCAACGAC GGATAAATTA TCGCAGAGCC
    AAGAGCGAAG TGGATGGTAG TCAGATGAGA AGTACATCAG TGATGGAAGA CGAAGAAAAT
    CTAGCAGTGT TAAGAAGACA TGTCATGAAT GAACTTATTG AGACAGAAAG GGCATATGTG
    GAAGAACTTC TATCTGTGAT TGAGGGTTAT GCTGCTGAAA TGAATAATCC TCTAGTGATG
    GACCTTATCC CCTCAGTGCT CCAGAATAAG AAGGATGTTC TTTTCGGCAA CATGGAAGAA
    ATTTACCAGT TTCATAACAG AATATTTCTG AGAGAGCTGG AACATTATAG AGACTATCCA
    GAACTAGTGG GAAGGTGTTT TTTGGAAAGG ATGGAAGATT TCCAGATCTA TGAAAAGTAC
    TGCCAGAACA AGCCACGGTC AGAGAGTTTG TGGAGGCAAT GCTCCGATGC TGCTTTCTTC
    CAGGATTGCC AAAAGAAACT GGACCATAAA CTCAGCCTGG ATTCCTATCT TCTAAAGCCG
    GTGCAAAGGA TAACAAAGTA TCAGCTTCTG TTAAAGGAAA TGCTTAAGTA TAGTAAAAAC
    TGTGAAGGAG CTGAAGATCT TCAGGAAGCA CTTGCCTCTA TATTGGGCAT TCTTAAAGCT
    GTCAATGATT CCATGCATCA GATAGCAATC ACTGGATATG AGGGGAACTT ACATGAACTT
    GGTAAACTCT TAATGCAAGG ATCATTTAAT GTATGGACAG AGCACAAGAA GGGCCATTCC
    AAAGTAAAGG ACCTTGCTAG GTTTAAACCA ATGCAAAGAC ACCTGTTTCT GCATGAAAAA
    GCTGTGCTGT TCTGCAAGAA GAGGGATGAG AATGGGGAGG GCTATGAGAA GGCACCATCC
    TACAGCTACA AACATTCCTT GAATATGATG TTTGTTGGAA TTACTGAGTA TGTAAAAGGA
    GACAATAGGA AATTTGAAAT TTGGTACAAC GCAAGGGAGG AGGTTTATAT TGTGCAGGCA
    CCAACCACAG AAGTAAAGGC CACCTGGGTA AATGAAATCA GAAAGGTGCT GACAAGCCAG
    TTAAAGGCAC ACAGAGATGC CAGCCACCAT AAATCGGCAG AGCACGCAAA CAGCTTACCT
    ACTCCTCTCC CATCCAGTAC TAGTCCCCTG AGAAACAAAT CTGGGAAGAT CAAAAAGCTG
    GAGGAAAGAA AAACTGAGCC TTTATTGAAT CCGGATGTCT CCTTAACATC TGTACCAAAA
    TACCTTGAAA AAGGCAAAGA TGGCACAGTA ACTAGTCCTA CCTCAGACTC TTCTGCCTTG
    TCAAAAAAGC GGTTCACTTT GCAGGGTTTT TCAAACCTCA AAAGTCAGAA AGCTTCTCCT
    ACCAGCCCAG ACAAAAAAGC TAAACGCCAT GAAGTAAAGA GTGACCCAAC TCCCTTTGGT
    TTAAGAGGTT GGAACAGAGC ATCACATTCA CTTGATGTCT CTGAAGAAAA TAATGAAGAT
    TGGTCTAGCG GAGAGGATCC AATTAACTCT TCAGATGCAG AAGAAGATGG AGGAGGTGAT
    GCACCAGAGA AAAAGCTAAC TCCTGGTAAA TATACTGTTG TGGCTGATTA CGAGAGAGGT
    TCAGATGAGC TGCTGGTAAA GAGTGGAGAT CTTGTTCACA TAATACATGA AGAGGATGGT
    GGAATGTGGT ATGTGAAGAA CCTGAGTACC AATAAGGAAG GTCTGGTACC TGCTAGCAAC
    CTAATGACTC TCATAGGCAC TTCCAAATCT GCCCTCTCTT TAAGCAGCTC TGAATCGGGA
    CCCGCTTCAA GCACTTTAAG CACATCTTCT AGCTGTAGTG AGAGCTGCAA TGCAGGCACC
    ACCAGTTTCT CTGATATCAA GGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36980
    Clone ID Related Accession (Same CDS sequence) XM_012957904.2
    Accession Version XM_012957904.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCTTCTCC TACCAGCCCA
    GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT
    TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC
    GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG
    AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG
    CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG
    TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT
    CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA
    AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC
    TCTGATATCA AGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813358.1
    CDS672..4229
    Translation

    Target ORF information:

    RefSeq Version XM_012957904.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957904.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGCTTCTCC TACCAGCCCA
    GACAAAAAAG CTAAACGCCA TGAAGTAAAG AGTGACCCAA CTCCCTTTGG TTTAAGAGGT
    TGGAACAGAG CATCACATTC ACTTGATGTC TCTGAAGAAA ATAATGAAGA TTGGTCTAGC
    GGAGAGGATC CAATTAACTC TTCAGATGCA GAAGAAGATG GAGGAGGTGA TGCACCAGAG
    AAAAAGCTAA CTCCTGGTAA ATATACTGTT GTGGCTGATT ACGAGAGAGG TTCAGATGAG
    CTGCTGGTAA AGAGTGGAGA TCTTGTTCAC ATAATACATG AAGAGGATGG TGGAATGTGG
    TATGTGAAGA ACCTGAGTAC CAATAAGGAA GGTCTGGTAC CTGCTAGCAA CCTAATGACT
    CTCATAGGCA CTTCCAAATC TGCCCTCTCT TTAAGCAGCT CTGAATCGGG ACCCGCTTCA
    AGCACTTTAA GCACATCTTC TAGCTGTAGT GAGAGCTGCA ATGCAGGCAC CACCAGTTTC
    TCTGATATCA AGGGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36982
    Clone ID Related Accession (Same CDS sequence) XM_012957906.2
    Accession Version XM_012957906.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813360.1
    CDS684..4136
    Translation

    Target ORF information:

    RefSeq Version XM_012957906.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis MCF.2 cell line derived transforming sequence-like (mcf2l), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957906.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGAGT GCTGGGGACT CTTGATATGC AGGGCACTAG GCAGCGGCAA CTGTTTTGCT 
    GCCTTCAGAC CCTGCTCTGA GGAGGAAGTG GATGTAAATG ATCACAAGGT GGTTGTGCCT
    GATATCATCC AACTTGTCCC TGATGATCAG TCGGAACTAG AATTGCCTTC AGATGACATC
    ATGCAACAGG AGACCTTTCC AATCTGTGCA GCCGATATTG AGGATCAGCT AAGAAAGCAG
    TTTGCCTACT TGTCAGGTGG AAGAGGGGAA AATGGCTGTC CAGTCGTCAC ATTCCCTGAA
    TACCCAGCAT TTAGCGAGAT TCCAGAAAAA GAGTTCCAGA ATGTACTCAC CTATCTGACA
    AGTGTCCCAA TTTTACAAGA TGCGGGAATT GGCTTCATCT TAGTGATAGA CAGAAGGCTG
    GACAAATGGA CCTCAGTGAA AGCCTCTATC CTGAGGATTG CGGCATCTTT TCCTGGGAAT
    TTACAGCTTG TTATAGTCCT GCGCCCATCA GGACTATTGC AGCGCACCCT CTCGGATATT
    GCATTCAAAT TCAACAAAGA TGACTTTAAA ATGAAAGTAC CGATCATAAT GCTGAATTCA
    GCAACAGAAC TCCATAGTTA TATTGACAAG ACCCAGCTGA CTCGAGATCT TGGTGGCACT
    CTGGATTACT GCCATACTAA CTGGCTCTCA CATCGCACTG CTATTGAAAA CTTTGCTGTC
    ATGGTGAAAC AAACAGCCCA AATGCTCCAG TCTTTTGGGA CAGAGCTGGC AGAGACAGAG
    CTGCCAAATG ATGTTCCCTC TACAAGCTTG CTGCTCCACG CACACACAGA GAAAAAGGAA
    AAAATGAAGG ATCACCTTAA GATAGCACTG ACACAGGGAT GTGACATTTT GAACAAGATC
    ATGGAGCCAC GTAAAGAGAA CCCTGAGTAT AGGATGAATC AGGACCAACT GGATAACCAA
    ACTACTGTGG AAAGGCTTCT TTTGCAATTA AATGAAACAG AAGCTGCTTT TGATGAGTTT
    TGGTCTAAAC ATGAACAGAA GCTTGAACAG TGTTTACAGC TGCGTCATTT TGAGTTAAAC
    TTCAGAGAGA TTTCTACAAG TCTGGATGGT ATTTCTGAGA GGCTAGAAGC TTTTACAGAT
    GTTGGAAACA GCTGCTCCCA TGCTGAACAC ATCCTGAAGG ACTTCTGTAA CTTTGAAGTT
    AAATCCCGAG AAGTTTTACA GACAGCCCAG AATTTAGTCA GAGAAGGTGA TCAACTCATT
    AAAAGTAATC ACTATGCTGT GGACTCCATT CTTCCAAAAT GCGACGAGAT TGGTCACTTA
    TGTGACAGCA TTAGCAACAC TATTCAGCAG AGACGGCAAA ACCTTCACAA ATCCTTGGAA
    CTTCACAGAC ATTTGGAGAA GACAATGAAG TGGTGTGATG AAGGGATTTT CTTGCTGGCG
    TCCCAACCTG TGGATAAGTT CCAGTCTCAG GATGGGGCCG AAGCTGCACT TCAGGAGATT
    ACAAGGTTTC TAGAGACTGG AACAGATATA ACCATGAAAG ATTTGGCTTT CATTTTGAAG
    ATGTACAACT CGATTCTCAC TCCAGAACTC CAGGAACATG TCAATAAGGT ATACCAGAAG
    CAGGAAAGCA TGCAGGAGAT GTTTGAGAAA AGGAAAGGTA GCCTTAGAAA ACTTGCAGCA
    AAGCAAACCA GACCTGTCCA GCCTGTTGCA CCTAGACCAG AAGCAATTTC CAAGTCTCCT
    ACCTCCTCTC CAGGTCTTCA TAGGTCTTCT GAAAGCAGAT ATAGCTCAGG AAGTATTCAA
    CGACGGATAA ATTATCGCAG AGCCAAGAGC GAAGTGGATG GTAGTCAGAT GAGAAGTACA
    TCAGTGATGG AAGACGAAGA AAATCTAGCA GTGTTAAGAA GACATGTCAT GAATGAACTT
    ATTGAGACAG AAAGGGCATA TGTGGAAGAA CTTCTATCTG TGATTGAGGG TTATGCTGCT
    GAAATGAATA ATCCTCTAGT GATGGACCTT ATCCCCTCAG TGCTCCAGAA TAAGAAGGAT
    GTTCTTTTCG GCAACATGGA AGAAATTTAC CAGTTTCATA ACAGAATATT TCTGAGAGAG
    CTGGAACATT ATAGAGACTA TCCAGAACTA GTGGGAAGGT GTTTTTTGGA AAGGATGGAA
    GATTTCCAGA TCTATGAAAA GTACTGCCAG AACAAGCCAC GGTCAGAGAG TTTGTGGAGG
    CAATGCTCCG ATGCTGCTTT CTTCCAGGAT TGCCAAAAGA AACTGGACCA TAAACTCAGC
    CTGGATTCCT ATCTTCTAAA GCCGGTGCAA AGGATAACAA AGTATCAGCT TCTGTTAAAG
    GAAATGCTTA AGTATAGTAA AAACTGTGAA GGAGCTGAAG ATCTTCAGGA AGCACTTGCC
    TCTATATTGG GCATTCTTAA AGCTGTCAAT GATTCCATGC ATCAGATAGC AATCACTGGA
    TATGAGGGGA ACTTACATGA ACTTGGTAAA CTCTTAATGC AAGGATCATT TAATGTATGG
    ACAGAGCACA AGAAGGGCCA TTCCAAAGTA AAGGACCTTG CTAGGTTTAA ACCAATGCAA
    AGACACCTGT TTCTGCATGA AAAAGCTGTG CTGTTCTGCA AGAAGAGGGA TGAGAATGGG
    GAGGGCTATG AGAAGGCACC ATCCTACAGC TACAAACATT CCTTGAATAT GATGTTTGTT
    GGAATTACTG AGTATGTAAA AGGAGACAAT AGGAAATTTG AAATTTGGTA CAACGCAAGG
    GAGGAGGTTT ATATTGTGCA GGCACCAACC ACAGAAGTAA AGGCCACCTG GGTAAATGAA
    ATCAGAAAGG TGCTGACAAG CCAGTTAAAG GCACACAGAG ATGCCAGCCA CCATAAATCG
    GCAGAGCACG CAAACAGCTT ACCTACTCCT CTCCCATCCA GTACTAGTCC CCTGAGAAAC
    AAATCTGGGA AGATCAAAAA GCTGGAGGAA AGAAAAACTG AGCCTTTATT GAATCCGGAT
    GTCTCCTTAA CATCTGTACC AAAATACCTT GAAAAAGGCA AAGATGGCAC AGTAACTAGT
    CCTACCTCAG ACTCTTCTGC CTTGTCAAAA AAGCGGTTCA CTTTGCAGGG TTTTTCAAAC
    CTCAAAAGTC AGAAAGCTTC TCCTACCAGC CCAGACAAAA AAGCTAAACG CCATGAAGTA
    AAGAGTGACC CAACTCCCTT TGGTTTAAGA GGTTGGAACA GAGCATCACA TTCACTTGAT
    GTCTCTGAAG AAAATAATGA AGATTGGTCT AGCGGAGAGG ATCCAATTAA CTCTTCAGAT
    GCAGAAGAAG ATGGAGGAGG TGATGCACCA GAGAAAAAGC TAACTCCTGG TAAATATACT
    GTTGTGGCTG ATTACGAGAG AGGTTCAGAT GAGCTGCTGG TAAAGAGTGG AGATCTTGTT
    CACATAATAC ATGAAGAGGA TGGTGGAATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.