rapgef4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef4
Entrez Gene ID	100497690
Full Name	Rap guanine nucleotide exchange factor 4
General protein information	Preferred Names Rap guanine nucleotide exchange factor 4 Names rap guanine nucleotide exchange factor 4 Rap guanine nucleotide exchange factor (GEF) 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004917715.2	XP_004917772.1	rap guanine nucleotide exchange factor 4 isoform X2
XM_004917715.3	XP_004917772.2	rap guanine nucleotide exchange factor 4 isoform X3
XM_031893351.1	XP_031749211.1	rap guanine nucleotide exchange factor 4 isoform X4
XM_002934617.4	XP_002934663.3	rap guanine nucleotide exchange factor 4 isoform X2
XM_031893353.1	XP_031749213.1	rap guanine nucleotide exchange factor 4 isoform X5
XM_002934617.3	XP_002934663.2	rap guanine nucleotide exchange factor 4 isoform X1
XM_031893350.1	XP_031749210.1	rap guanine nucleotide exchange factor 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rapgef4	XP_006234430.1
Xenopus tropicalis (tropical clawed frog)	rapgef4	XP_002934663.2
Pan troglodytes (chimpanzee)	RAPGEF4	XP_003309469.1
Canis lupus familiaris (dog)	RAPGEF4	XP_005640372.1
Mus musculus (house mouse)	Rapgef4	NP_001191094.1
Homo sapiens (human)	RAPGEF4	NP_008954.2
	RAPGEF4	XP_001086678.2
Bos taurus (cattle)	RAPGEF4	NP_001179831.1
Gallus gallus (chicken)	RAPGEF4	XP_426579.3
Danio rerio (zebrafish)	rapgef4	XP_687578.4
Drosophila melanogaster (fruit fly)	Epac	NP_001097202.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007307	XP_308514.4
Caenorhabditis elegans (roundworm)	epac-1	NP_499256.2

Gene Ontology

The following rapgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa66858	XM_031893351.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66859	XM_031893353.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66855	XM_031893350.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa66856	XM_002934617.4 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa66857	XM_004917715.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa15917	XM_002934617.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa15918	XM_004917715.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef4 ( XM_031893351.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749211.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa66858
Clone ID Related Accession (Same CDS sequence)	XM_031893351.1
Accession Version	XM_031893351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCT TCCTGGCTCC ACCCTACGGT GTTCTGGAAA CCGGATCTAA TGACAGGCTC 
TCAGACAAAG AAAATATGAA TAGCGCTCTG GGACCAGGAT CCAAAAACTG TAATAAGACC 
CCACTCATTG AAAATCAAAT CCCGATACGT CCAGCTAAAA CCATTACAGA GGTAATGGGA 
TTTCGCCAAG TGGTGAAGGT TCCATCAGAA AGAATACTCA GGGCTGGAAA GACTTTGCGT 
AATGCCATCC TTACTCGGGC GCCCCACATG ATTAGGGATC GAAAGTATCA TCTGAAAACA 
TACAGGCAGT GCTGTGTGGG AACTGAACTT GTTGACTGGC TTATGCAGCA AAGCTCATGC 
GTTCATTCTC GGACGCAAGC AGTGGGAATG TGGCAGGTGT TGCTCGAAGA TGGAGTCCTA 
AACCATGTGG ACCAGGAATA TTACTTCCAA GACAAATACC TGTTTTATCG GTTTCTTGAC 
GATGAACACG AGGATGCACC GATGCCAACA GAGGAGGAGA AGAAAGAGAG CGACGAGGAG 
CTGCAGGATA CGTTGCTCTT ACTCTCGCAG ATAGGACCCG ATGCACACAT GAGAATGATT 
CTGAGAAAAC CACCGGGTCA GAGGAGTGCA GATGACTTAG AAATTATTTT TGAAGAACTT 
ATTCACATTA AAGCCTTATC TCACTTATCA ACAACAGTGA AAAGAGAACT TGCTGGATTC 
CTAATCTTTG AATCTCATCC CAAAGCAGGA ACTGTGTTAT TTAACCAAGG AGAAGAGGGC 
ACTTCCTGGT ACATCATCTT GAAAGGATCT GTCAATGTTG TCATCTATGG AAAGGGAGTT 
GTTTGTACCC TGCATGAGGG AGATGACTTT GGGAAGCTTG CCCTTGTTAA CGACGCCCCA 
AGGGCAGCAT CCATTGTTTT ACGAGAAGAC AATTGTCACT TCCTGCGTGT GGACAAGGAG 
GACTTCAACC GGATCCTGCG TGACGTGGAG GCCAACACAG TGAGACTCAA GGAGCACGAT 
CAAGATGTCT TGGTACTAGA AAAAATCCTG GCTGGATCTC AAGGCTCAGC ACAAGGGAAC 
ACTCAGTCGC CATATAAATA CACTGTGATG TCAGGAAACC CAGAGAAACT TCTAGAACAC 
TTCCTGGAAA CAATGCGCCT TGATCCGAGT TTAAATGATG CAACAGACAC CGCTTTGGAC 
GACTTTATTC TGATGCACTG TGTTTTTATG CCAAATTCTC AGCTCTGCCC GGCCTTGATG 
TCACATTACC ATGCGCAGCC TTCCCAGGGA ACGGAGCAGG AGAAGCTGGA TTACACCCTG 
AATAACAAGC GCAGGGTCAT ACGGCTGGTT CAGCAGTGGG CCACTCTGTA TGGGGACCTG 
CTCCGTGAGG ATGAGGTTTC AATGGCCTTT CTTGAAGAAT TTTATGTGTC GGTATCAGAT 
GACACAAGAA CGACTGCAGC CCTGAAGGAG CAGCTACCTG ACCTAGAACG AGCCATCAAG 
CAAATATCTG AGGATGCAAA GGGGCAACAA AAGAAGCACA AGGTGCTTTT ACGGCAATTC 
AGCACTGGAG ATGAGAGACT GCAAAAGCGC CAGGCAATAC GGAGTTCCGA TGAACTTCTG 
TTCAAGGTAT ACTGCATAGA CCATACGTAC ACCACTATCC GTGTCGCTGT GGCTGCTTCA 
GTGAAAGAAG TGCTCTCTGC GGTCGCCGAT AAACTAGGCT CCGGGGACAG CTTGATACTC 
GTCAAAATCA GTTCTGCAGG AGAAAAAGTG GTGCTGAAAC CAAATGAAAT ATCCGTCTTT 
ACAACGCTCA GCGTGAATGG ACGTCTGTTT GCCTGCCCCC GGGACCAGTT CGATTCTTTG 
ACCCCTCTAC CAGAACAAGA GGGGCCTTCC GCTGGAACCA TGTCAACTTT TGAACTCATG 
AGCTCTAAAG ACTTGGCGTA TCAGATGACC ATTCATGACT GGGACCTATT CAGTTGTGTG 
CATGAGCTGG AACTTATTTA CCACACATTC GGGCGACAGA ATTTCAAAAA GACGACGGCA 
AATTTGGACC TCTTCCTGAG ACGTTTTAAT GAAATTCAGT TCTGGGTTGT GACTGAGATT 
TGCCTGTGCC CTCAACTCAG CAAGCGCGTT CAACTTCTAA AGAAATTTAT TAAAATTGCG 
GCCCACTGCA AGGAATATAA AAATTTGAAC TCGTTCTTTG CTATTGTCAT GGGGCTGAGT 
AACGTGGCCG TAAGCCGTCT CTCACTGACA TGGGAGAAAC TCCCAAGCAA GTTCAAAAAG 
ATCTATGCAG AATTTGAAAA TTTAATGGAT CCATCAAGGA ATCACAGGGC TTACAGGCTC 
ACTGTAGCTA AACTGGACCC TCCAATCATT CCATTTACAC CTCTGCTTAT CAAAGATATG 
ACTTTTACCC ATGAGGGCAA CAAAACCTTC ATTGACAATT TGGTTAACTT TGAGAAAATG 
CGAATGATCG CAAACACCTT GAGAACAATA AGATACTGCC GAAGCGTCCC TTTCAATCCT 
GATGCCTCTC TTGCCGGCAA AAATCACCAA GACGTGCGGA ATTATGTGCG CCAGTTCAAC 
GTGATTGACA ACCAGAGGAC ACTGTCTCAG ATGTCCCACC GGCTGGAACC ACGCCGAGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749211.1
CDS	329..2971
Translation	MAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKTPLIENQIPIRPAKTITEVMGFRQVVKVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEDGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_031893351.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893351.1

ORF Insert Sequence:

ATGGCTGGCT TCCTGGCTCC ACCCTACGGT GTTCTGGAAA CCGGATCTAA TGACAGGCTC 
TCAGACAAAG AAAATATGAA TAGCGCTCTG GGACCAGGAT CCAAAAACTG TAATAAGACC 
CCACTCATTG AAAATCAAAT CCCGATACGT CCAGCTAAAA CCATTACAGA GGTAATGGGA 
TTTCGCCAAG TGGTGAAGGT TCCATCAGAA AGAATACTCA GGGCTGGAAA GACTTTGCGT 
AATGCCATCC TTACTCGGGC GCCCCACATG ATTAGGGATC GAAAGTATCA TCTGAAAACA 
TACAGGCAGT GCTGTGTGGG AACTGAACTT GTTGACTGGC TTATGCAGCA AAGCTCATGC 
GTTCATTCTC GGACGCAAGC AGTGGGAATG TGGCAGGTGT TGCTCGAAGA TGGAGTCCTA 
AACCATGTGG ACCAGGAATA TTACTTCCAA GACAAATACC TGTTTTATCG GTTTCTTGAC 
GATGAACACG AGGATGCACC GATGCCAACA GAGGAGGAGA AGAAAGAGAG CGACGAGGAG 
CTGCAGGATA CGTTGCTCTT ACTCTCGCAG ATAGGACCCG ATGCACACAT GAGAATGATT 
CTGAGAAAAC CACCGGGTCA GAGGAGTGCA GATGACTTAG AAATTATTTT TGAAGAACTT 
ATTCACATTA AAGCCTTATC TCACTTATCA ACAACAGTGA AAAGAGAACT TGCTGGATTC 
CTAATCTTTG AATCTCATCC CAAAGCAGGA ACTGTGTTAT TTAACCAAGG AGAAGAGGGC 
ACTTCCTGGT ACATCATCTT GAAAGGATCT GTCAATGTTG TCATCTATGG AAAGGGAGTT 
GTTTGTACCC TGCATGAGGG AGATGACTTT GGGAAGCTTG CCCTTGTTAA CGACGCCCCA 
AGGGCAGCAT CCATTGTTTT ACGAGAAGAC AATTGTCACT TCCTGCGTGT GGACAAGGAG 
GACTTCAACC GGATCCTGCG TGACGTGGAG GCCAACACAG TGAGACTCAA GGAGCACGAT 
CAAGATGTCT TGGTACTAGA AAAAATCCTG GCTGGATCTC AAGGCTCAGC ACAAGGGAAC 
ACTCAGTCGC CATATAAATA CACTGTGATG TCAGGAAACC CAGAGAAACT TCTAGAACAC 
TTCCTGGAAA CAATGCGCCT TGATCCGAGT TTAAATGATG CAACAGACAC CGCTTTGGAC 
GACTTTATTC TGATGCACTG TGTTTTTATG CCAAATTCTC AGCTCTGCCC GGCCTTGATG 
TCACATTACC ATGCGCAGCC TTCCCAGGGA ACGGAGCAGG AGAAGCTGGA TTACACCCTG 
AATAACAAGC GCAGGGTCAT ACGGCTGGTT CAGCAGTGGG CCACTCTGTA TGGGGACCTG 
CTCCGTGAGG ATGAGGTTTC AATGGCCTTT CTTGAAGAAT TTTATGTGTC GGTATCAGAT 
GACACAAGAA CGACTGCAGC CCTGAAGGAG CAGCTACCTG ACCTAGAACG AGCCATCAAG 
CAAATATCTG AGGATGCAAA GGGGCAACAA AAGAAGCACA AGGTGCTTTT ACGGCAATTC 
AGCACTGGAG ATGAGAGACT GCAAAAGCGC CAGGCAATAC GGAGTTCCGA TGAACTTCTG 
TTCAAGGTAT ACTGCATAGA CCATACGTAC ACCACTATCC GTGTCGCTGT GGCTGCTTCA 
GTGAAAGAAG TGCTCTCTGC GGTCGCCGAT AAACTAGGCT CCGGGGACAG CTTGATACTC 
GTCAAAATCA GTTCTGCAGG AGAAAAAGTG GTGCTGAAAC CAAATGAAAT ATCCGTCTTT 
ACAACGCTCA GCGTGAATGG ACGTCTGTTT GCCTGCCCCC GGGACCAGTT CGATTCTTTG 
ACCCCTCTAC CAGAACAAGA GGGGCCTTCC GCTGGAACCA TGTCAACTTT TGAACTCATG 
AGCTCTAAAG ACTTGGCGTA TCAGATGACC ATTCATGACT GGGACCTATT CAGTTGTGTG 
CATGAGCTGG AACTTATTTA CCACACATTC GGGCGACAGA ATTTCAAAAA GACGACGGCA 
AATTTGGACC TCTTCCTGAG ACGTTTTAAT GAAATTCAGT TCTGGGTTGT GACTGAGATT 
TGCCTGTGCC CTCAACTCAG CAAGCGCGTT CAACTTCTAA AGAAATTTAT TAAAATTGCG 
GCCCACTGCA AGGAATATAA AAATTTGAAC TCGTTCTTTG CTATTGTCAT GGGGCTGAGT 
AACGTGGCCG TAAGCCGTCT CTCACTGACA TGGGAGAAAC TCCCAAGCAA GTTCAAAAAG 
ATCTATGCAG AATTTGAAAA TTTAATGGAT CCATCAAGGA ATCACAGGGC TTACAGGCTC 
ACTGTAGCTA AACTGGACCC TCCAATCATT CCATTTACAC CTCTGCTTAT CAAAGATATG 
ACTTTTACCC ATGAGGGCAA CAAAACCTTC ATTGACAATT TGGTTAACTT TGAGAAAATG 
CGAATGATCG CAAACACCTT GAGAACAATA AGATACTGCC GAAGCGTCCC TTTCAATCCT 
GATGCCTCTC TTGCCGGCAA AAATCACCAA GACGTGCGGA ATTATGTGCG CCAGTTCAAC 
GTGATTGACA ACCAGAGGAC ACTGTCTCAG ATGTCCCACC GGCTGGAACC ACGCCGAGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_031893353.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749213.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa66859
Clone ID Related Accession (Same CDS sequence)	XM_031893353.1
Accession Version	XM_031893353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCT TCCTGGCTCC ACCCTACGGT GTTCTGGAAA CCGGATCTAA TGACAGGCTC 
TCAGACAAAG AAAATATGAA TAGCGCTCTG GGACCAGGAT CCAAAAACTG TAATAAGACC 
CCACTCATTG AAAATCAAAT CCCGATACGT CCAGCTAAAA CCATTACAGA GGTTCCATCA 
GAAAGAATAC TCAGGGCTGG AAAGACTTTG CGTAATGCCA TCCTTACTCG GGCGCCCCAC 
ATGATTAGGG ATCGAAAGTA TCATCTGAAA ACATACAGGC AGTGCTGTGT GGGAACTGAA 
CTTGTTGACT GGCTTATGCA GCAAAGCTCA TGCGTTCATT CTCGGACGCA AGCAGTGGGA 
ATGTGGCAGG TGTTGCTCGA AGATGGAGTC CTAAACCATG TGGACCAGGA ATATTACTTC 
CAAGACAAAT ACCTGTTTTA TCGGTTTCTT GACGATGAAC ACGAGGATGC ACCGATGCCA 
ACAGAGGAGG AGAAGAAAGA GAGCGACGAG GAGCTGCAGG ATACGTTGCT CTTACTCTCG 
CAGATAGGAC CCGATGCACA CATGAGAATG ATTCTGAGAA AACCACCGGG TCAGAGGAGT 
GCAGATGACT TAGAAATTAT TTTTGAAGAA CTTATTCACA TTAAAGCCTT ATCTCACTTA 
TCAACAACAG TGAAAAGAGA ACTTGCTGGA TTCCTAATCT TTGAATCTCA TCCCAAAGCA 
GGAACTGTGT TATTTAACCA AGGAGAAGAG GGCACTTCCT GGTACATCAT CTTGAAAGGA 
TCTGTCAATG TTGTCATCTA TGGAAAGGGA GTTGTTTGTA CCCTGCATGA GGGAGATGAC 
TTTGGGAAGC TTGCCCTTGT TAACGACGCC CCAAGGGCAG CATCCATTGT TTTACGAGAA 
GACAATTGTC ACTTCCTGCG TGTGGACAAG GAGGACTTCA ACCGGATCCT GCGTGACGTG 
GAGGCCAACA CAGTGAGACT CAAGGAGCAC GATCAAGATG TCTTGGTACT AGAAAAAATC 
CTGGCTGGAT CTCAAGGCTC AGCACAAGGG AACACTCAGT CGCCATATAA ATACACTGTG 
ATGTCAGGAA ACCCAGAGAA ACTTCTAGAA CACTTCCTGG AAACAATGCG CCTTGATCCG 
AGTTTAAATG ATGCAACAGA CACCGCTTTG GACGACTTTA TTCTGATGCA CTGTGTTTTT 
ATGCCAAATT CTCAGCTCTG CCCGGCCTTG ATGTCACATT ACCATGCGCA GCCTTCCCAG 
GGAACGGAGC AGGAGAAGCT GGATTACACC CTGAATAACA AGCGCAGGGT CATACGGCTG 
GTTCAGCAGT GGGCCACTCT GTATGGGGAC CTGCTCCGTG AGGATGAGGT TTCAATGGCC 
TTTCTTGAAG AATTTTATGT GTCGGTATCA GATGACACAA GAACGACTGC AGCCCTGAAG 
GAGCAGCTAC CTGACCTAGA ACGAGCCATC AAGCAAATAT CTGAGGATGC AAAGGGGCAA 
CAAAAGAAGC ACAAGGTGCT TTTACGGCAA TTCAGCACTG GAGATGAGAG ACTGCAAAAG 
CGCCAGGCAA TACGGAGTTC CGATGAACTT CTGTTCAAGG TATACTGCAT AGACCATACG 
TACACCACTA TCCGTGTCGC TGTGGCTGCT TCAGTGAAAG AAGTGCTCTC TGCGGTCGCC 
GATAAACTAG GCTCCGGGGA CAGCTTGATA CTCGTCAAAA TCAGTTCTGC AGGAGAAAAA 
GTGGTGCTGA AACCAAATGA AATATCCGTC TTTACAACGC TCAGCGTGAA TGGACGTCTG 
TTTGCCTGCC CCCGGGACCA GTTCGATTCT TTGACCCCTC TACCAGAACA AGAGGGGCCT 
TCCGCTGGAA CCATGTCAAC TTTTGAACTC ATGAGCTCTA AAGACTTGGC GTATCAGATG 
ACCATTCATG ACTGGGACCT ATTCAGTTGT GTGCATGAGC TGGAACTTAT TTACCACACA 
TTCGGGCGAC AGAATTTCAA AAAGACGACG GCAAATTTGG ACCTCTTCCT GAGACGTTTT 
AATGAAATTC AGTTCTGGGT TGTGACTGAG ATTTGCCTGT GCCCTCAACT CAGCAAGCGC 
GTTCAACTTC TAAAGAAATT TATTAAAATT GCGGCCCACT GCAAGGAATA TAAAAATTTG 
AACTCGTTCT TTGCTATTGT CATGGGGCTG AGTAACGTGG CCGTAAGCCG TCTCTCACTG 
ACATGGGAGA AACTCCCAAG CAAGTTCAAA AAGATCTATG CAGAATTTGA AAATTTAATG 
GATCCATCAA GGAATCACAG GGCTTACAGG CTCACTGTAG CTAAACTGGA CCCTCCAATC 
ATTCCATTTA CACCTCTGCT TATCAAAGAT ATGACTTTTA CCCATGAGGG CAACAAAACC 
TTCATTGACA ATTTGGTTAA CTTTGAGAAA ATGCGAATGA TCGCAAACAC CTTGAGAACA 
ATAAGATACT GCCGAAGCGT CCCTTTCAAT CCTGATGCCT CTCTTGCCGG CAAAAATCAC 
CAAGACGTGC GGAATTATGT GCGCCAGTTC AACGTGATTG ACAACCAGAG GACACTGTCT 
CAGATGTCCC ACCGGCTGGA ACCACGCCGA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749213.1
CDS	329..2944
Translation	MAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKTPLIENQIPIRPAKTITEVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEDGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_031893353.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893353.1

ORF Insert Sequence:

ATGGCTGGCT TCCTGGCTCC ACCCTACGGT GTTCTGGAAA CCGGATCTAA TGACAGGCTC 
TCAGACAAAG AAAATATGAA TAGCGCTCTG GGACCAGGAT CCAAAAACTG TAATAAGACC 
CCACTCATTG AAAATCAAAT CCCGATACGT CCAGCTAAAA CCATTACAGA GGTTCCATCA 
GAAAGAATAC TCAGGGCTGG AAAGACTTTG CGTAATGCCA TCCTTACTCG GGCGCCCCAC 
ATGATTAGGG ATCGAAAGTA TCATCTGAAA ACATACAGGC AGTGCTGTGT GGGAACTGAA 
CTTGTTGACT GGCTTATGCA GCAAAGCTCA TGCGTTCATT CTCGGACGCA AGCAGTGGGA 
ATGTGGCAGG TGTTGCTCGA AGATGGAGTC CTAAACCATG TGGACCAGGA ATATTACTTC 
CAAGACAAAT ACCTGTTTTA TCGGTTTCTT GACGATGAAC ACGAGGATGC ACCGATGCCA 
ACAGAGGAGG AGAAGAAAGA GAGCGACGAG GAGCTGCAGG ATACGTTGCT CTTACTCTCG 
CAGATAGGAC CCGATGCACA CATGAGAATG ATTCTGAGAA AACCACCGGG TCAGAGGAGT 
GCAGATGACT TAGAAATTAT TTTTGAAGAA CTTATTCACA TTAAAGCCTT ATCTCACTTA 
TCAACAACAG TGAAAAGAGA ACTTGCTGGA TTCCTAATCT TTGAATCTCA TCCCAAAGCA 
GGAACTGTGT TATTTAACCA AGGAGAAGAG GGCACTTCCT GGTACATCAT CTTGAAAGGA 
TCTGTCAATG TTGTCATCTA TGGAAAGGGA GTTGTTTGTA CCCTGCATGA GGGAGATGAC 
TTTGGGAAGC TTGCCCTTGT TAACGACGCC CCAAGGGCAG CATCCATTGT TTTACGAGAA 
GACAATTGTC ACTTCCTGCG TGTGGACAAG GAGGACTTCA ACCGGATCCT GCGTGACGTG 
GAGGCCAACA CAGTGAGACT CAAGGAGCAC GATCAAGATG TCTTGGTACT AGAAAAAATC 
CTGGCTGGAT CTCAAGGCTC AGCACAAGGG AACACTCAGT CGCCATATAA ATACACTGTG 
ATGTCAGGAA ACCCAGAGAA ACTTCTAGAA CACTTCCTGG AAACAATGCG CCTTGATCCG 
AGTTTAAATG ATGCAACAGA CACCGCTTTG GACGACTTTA TTCTGATGCA CTGTGTTTTT 
ATGCCAAATT CTCAGCTCTG CCCGGCCTTG ATGTCACATT ACCATGCGCA GCCTTCCCAG 
GGAACGGAGC AGGAGAAGCT GGATTACACC CTGAATAACA AGCGCAGGGT CATACGGCTG 
GTTCAGCAGT GGGCCACTCT GTATGGGGAC CTGCTCCGTG AGGATGAGGT TTCAATGGCC 
TTTCTTGAAG AATTTTATGT GTCGGTATCA GATGACACAA GAACGACTGC AGCCCTGAAG 
GAGCAGCTAC CTGACCTAGA ACGAGCCATC AAGCAAATAT CTGAGGATGC AAAGGGGCAA 
CAAAAGAAGC ACAAGGTGCT TTTACGGCAA TTCAGCACTG GAGATGAGAG ACTGCAAAAG 
CGCCAGGCAA TACGGAGTTC CGATGAACTT CTGTTCAAGG TATACTGCAT AGACCATACG 
TACACCACTA TCCGTGTCGC TGTGGCTGCT TCAGTGAAAG AAGTGCTCTC TGCGGTCGCC 
GATAAACTAG GCTCCGGGGA CAGCTTGATA CTCGTCAAAA TCAGTTCTGC AGGAGAAAAA 
GTGGTGCTGA AACCAAATGA AATATCCGTC TTTACAACGC TCAGCGTGAA TGGACGTCTG 
TTTGCCTGCC CCCGGGACCA GTTCGATTCT TTGACCCCTC TACCAGAACA AGAGGGGCCT 
TCCGCTGGAA CCATGTCAAC TTTTGAACTC ATGAGCTCTA AAGACTTGGC GTATCAGATG 
ACCATTCATG ACTGGGACCT ATTCAGTTGT GTGCATGAGC TGGAACTTAT TTACCACACA 
TTCGGGCGAC AGAATTTCAA AAAGACGACG GCAAATTTGG ACCTCTTCCT GAGACGTTTT 
AATGAAATTC AGTTCTGGGT TGTGACTGAG ATTTGCCTGT GCCCTCAACT CAGCAAGCGC 
GTTCAACTTC TAAAGAAATT TATTAAAATT GCGGCCCACT GCAAGGAATA TAAAAATTTG 
AACTCGTTCT TTGCTATTGT CATGGGGCTG AGTAACGTGG CCGTAAGCCG TCTCTCACTG 
ACATGGGAGA AACTCCCAAG CAAGTTCAAA AAGATCTATG CAGAATTTGA AAATTTAATG 
GATCCATCAA GGAATCACAG GGCTTACAGG CTCACTGTAG CTAAACTGGA CCCTCCAATC 
ATTCCATTTA CACCTCTGCT TATCAAAGAT ATGACTTTTA CCCATGAGGG CAACAAAACC 
TTCATTGACA ATTTGGTTAA CTTTGAGAAA ATGCGAATGA TCGCAAACAC CTTGAGAACA 
ATAAGATACT GCCGAAGCGT CCCTTTCAAT CCTGATGCCT CTCTTGCCGG CAAAAATCAC 
CAAGACGTGC GGAATTATGT GCGCCAGTTC AACGTGATTG ACAACCAGAG GACACTGTCT 
CAGATGTCCC ACCGGCTGGA ACCACGCCGA GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_031893350.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749210.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66855
Clone ID Related Accession (Same CDS sequence)	XM_031893350.1
Accession Version	XM_031893350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTAATGGGAT TTCGCCAAGT GGTGAAGGTT 
CCATCAGAAA GAATACTCAG GGCTGGAAAG ACTTTGCGTA ATGCCATCCT TACTCGGGCG 
CCCCACATGA TTAGGGATCG AAAGTATCAT CTGAAAACAT ACAGGCAGTG CTGTGTGGGA 
ACTGAACTTG TTGACTGGCT TATGCAGCAA AGCTCATGCG TTCATTCTCG GACGCAAGCA 
GTGGGAATGT GGCAGGTGTT GCTCGAAGAT GGAGTCCTAA ACCATGTGGA CCAGGAATAT 
TACTTCCAAG ACAAATACCT GTTTTATCGG TTTCTTGACG ATGAACACGA GGATGCACCG 
ATGCCAACAG AGGAGGAGAA GAAAGAGAGC GACGAGGAGC TGCAGGATAC GTTGCTCTTA 
CTCTCGCAGA TAGGACCCGA TGCACACATG AGAATGATTC TGAGAAAACC ACCGGGTCAG 
AGGAGTGCAG ATGACTTAGA AATTATTTTT GAAGAACTTA TTCACATTAA AGCCTTATCT 
CACTTATCAA CAACAGTGAA AAGAGAACTT GCTGGATTCC TAATCTTTGA ATCTCATCCC 
AAAGCAGGAA CTGTGTTATT TAACCAAGGA GAAGAGGGCA CTTCCTGGTA CATCATCTTG 
AAAGGATCTG TCAATGTTGT CATCTATGGA AAGGGAGTTG TTTGTACCCT GCATGAGGGA 
GATGACTTTG GGAAGCTTGC CCTTGTTAAC GACGCCCCAA GGGCAGCATC CATTGTTTTA 
CGAGAAGACA ATTGTCACTT CCTGCGTGTG GACAAGGAGG ACTTCAACCG GATCCTGCGT 
GACGTGGAGG CCAACACAGT GAGACTCAAG GAGCACGATC AAGATGTCTT GGTACTAGAA 
AAAATCCTGG CTGGATCTCA AGGCTCAGCA CAAGGGAACA CTCAGTCGCC ATATAAATAC 
ACTGTGATGT CAGGAAACCC AGAGAAACTT CTAGAACACT TCCTGGAAAC AATGCGCCTT 
GATCCGAGTT TAAATGATGC AACAGACACC GCTTTGGACG ACTTTATTCT GATGCACTGT 
GTTTTTATGC CAAATTCTCA GCTCTGCCCG GCCTTGATGT CACATTACCA TGCGCAGCCT 
TCCCAGGGAA CGGAGCAGGA GAAGCTGGAT TACACCCTGA ATAACAAGCG CAGGGTCATA 
CGGCTGGTTC AGCAGTGGGC CACTCTGTAT GGGGACCTGC TCCGTGAGGA TGAGGTTTCA 
ATGGCCTTTC TTGAAGAATT TTATGTGTCG GTATCAGATG ACACAAGAAC GACTGCAGCC 
CTGAAGGAGC AGCTACCTGA CCTAGAACGA GCCATCAAGC AAATATCTGA GGATGCAAAG 
GGGCAACAAA AGAAGCACAA GGTGCTTTTA CGGCAATTCA GCACTGGAGA TGAGAGACTG 
CAAAAGCGCC AGGCAATACG GAGTTCCGAT GAACTTCTGT TCAAGGTATA CTGCATAGAC 
CATACGTACA CCACTATCCG TGTCGCTGTG GCTGCTTCAG TGAAAGAAGT GCTCTCTGCG 
GTCGCCGATA AACTAGGCTC CGGGGACAGC TTGATACTCG TCAAAATCAG TTCTGCAGGA 
GAAAAAGTGG TGCTGAAACC AAATGAAATA TCCGTCTTTA CAACGCTCAG CGTGAATGGA 
CGTCTGTTTG CCTGCCCCCG GGACCAGTTC GATTCTTTGA CCCCTCTACC AGAACAAGAG 
GGGCCTTCCG CTGGAACCAT GTCAACTTTT GAACTCATGA GCTCTAAAGA CTTGGCGTAT 
CAGATGACCA TTCATGACTG GGACCTATTC AGTTGTGTGC ATGAGCTGGA ACTTATTTAC 
CACACATTCG GGCGACAGAA TTTCAAAAAG ACGACGGCAA ATTTGGACCT CTTCCTGAGA 
CGTTTTAATG AAATTCAGTT CTGGGTTGTG ACTGAGATTT GCCTGTGCCC TCAACTCAGC 
AAGCGCGTTC AACTTCTAAA GAAATTTATT AAAATTGCGG CCCACTGCAA GGAATATAAA 
AATTTGAACT CGTTCTTTGC TATTGTCATG GGGCTGAGTA ACGTGGCCGT AAGCCGTCTC 
TCACTGACAT GGGAGAAACT CCCAAGCAAG TTCAAAAAGA TCTATGCAGA ATTTGAAAAT 
TTAATGGATC CATCAAGGAA TCACAGGGCT TACAGGCTCA CTGTAGCTAA ACTGGACCCT 
CCAATCATTC CATTTACACC TCTGCTTATC AAAGATATGA CTTTTACCCA TGAGGGCAAC 
AAAACCTTCA TTGACAATTT GGTTAACTTT GAGAAAATGC GAATGATCGC AAACACCTTG 
AGAACAATAA GATACTGCCG AAGCGTCCCT TTCAATCCTG ATGCCTCTCT TGCCGGCAAA 
AATCACCAAG ACGTGCGGAA TTATGTGCGC CAGTTCAACG TGATTGACAA CCAGAGGACA 
CTGTCTCAGA TGTCCCACCG GCTGGAACCA CGCCGAGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749210.1
CDS	285..3386
Translation	MVAAHTAHSTSSAEWIACLDKRPLERSGEDVDIIFARLKEVKAFERFHPNLLQQICFCGYYENLEKGITLYRQGDIGTSWYAVLTGSLDVKVSETNNHQDAVVICTLGVGTAFGESILDNTPRHATIVTRENSELLRIEQKDFKTLWEKYRQYMAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKTPLIENQIPIRPAKTITEVMGFRQVVKVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEDGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_031893350.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893350.1

ORF Insert Sequence:

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTAATGGGAT TTCGCCAAGT GGTGAAGGTT 
CCATCAGAAA GAATACTCAG GGCTGGAAAG ACTTTGCGTA ATGCCATCCT TACTCGGGCG 
CCCCACATGA TTAGGGATCG AAAGTATCAT CTGAAAACAT ACAGGCAGTG CTGTGTGGGA 
ACTGAACTTG TTGACTGGCT TATGCAGCAA AGCTCATGCG TTCATTCTCG GACGCAAGCA 
GTGGGAATGT GGCAGGTGTT GCTCGAAGAT GGAGTCCTAA ACCATGTGGA CCAGGAATAT 
TACTTCCAAG ACAAATACCT GTTTTATCGG TTTCTTGACG ATGAACACGA GGATGCACCG 
ATGCCAACAG AGGAGGAGAA GAAAGAGAGC GACGAGGAGC TGCAGGATAC GTTGCTCTTA 
CTCTCGCAGA TAGGACCCGA TGCACACATG AGAATGATTC TGAGAAAACC ACCGGGTCAG 
AGGAGTGCAG ATGACTTAGA AATTATTTTT GAAGAACTTA TTCACATTAA AGCCTTATCT 
CACTTATCAA CAACAGTGAA AAGAGAACTT GCTGGATTCC TAATCTTTGA ATCTCATCCC 
AAAGCAGGAA CTGTGTTATT TAACCAAGGA GAAGAGGGCA CTTCCTGGTA CATCATCTTG 
AAAGGATCTG TCAATGTTGT CATCTATGGA AAGGGAGTTG TTTGTACCCT GCATGAGGGA 
GATGACTTTG GGAAGCTTGC CCTTGTTAAC GACGCCCCAA GGGCAGCATC CATTGTTTTA 
CGAGAAGACA ATTGTCACTT CCTGCGTGTG GACAAGGAGG ACTTCAACCG GATCCTGCGT 
GACGTGGAGG CCAACACAGT GAGACTCAAG GAGCACGATC AAGATGTCTT GGTACTAGAA 
AAAATCCTGG CTGGATCTCA AGGCTCAGCA CAAGGGAACA CTCAGTCGCC ATATAAATAC 
ACTGTGATGT CAGGAAACCC AGAGAAACTT CTAGAACACT TCCTGGAAAC AATGCGCCTT 
GATCCGAGTT TAAATGATGC AACAGACACC GCTTTGGACG ACTTTATTCT GATGCACTGT 
GTTTTTATGC CAAATTCTCA GCTCTGCCCG GCCTTGATGT CACATTACCA TGCGCAGCCT 
TCCCAGGGAA CGGAGCAGGA GAAGCTGGAT TACACCCTGA ATAACAAGCG CAGGGTCATA 
CGGCTGGTTC AGCAGTGGGC CACTCTGTAT GGGGACCTGC TCCGTGAGGA TGAGGTTTCA 
ATGGCCTTTC TTGAAGAATT TTATGTGTCG GTATCAGATG ACACAAGAAC GACTGCAGCC 
CTGAAGGAGC AGCTACCTGA CCTAGAACGA GCCATCAAGC AAATATCTGA GGATGCAAAG 
GGGCAACAAA AGAAGCACAA GGTGCTTTTA CGGCAATTCA GCACTGGAGA TGAGAGACTG 
CAAAAGCGCC AGGCAATACG GAGTTCCGAT GAACTTCTGT TCAAGGTATA CTGCATAGAC 
CATACGTACA CCACTATCCG TGTCGCTGTG GCTGCTTCAG TGAAAGAAGT GCTCTCTGCG 
GTCGCCGATA AACTAGGCTC CGGGGACAGC TTGATACTCG TCAAAATCAG TTCTGCAGGA 
GAAAAAGTGG TGCTGAAACC AAATGAAATA TCCGTCTTTA CAACGCTCAG CGTGAATGGA 
CGTCTGTTTG CCTGCCCCCG GGACCAGTTC GATTCTTTGA CCCCTCTACC AGAACAAGAG 
GGGCCTTCCG CTGGAACCAT GTCAACTTTT GAACTCATGA GCTCTAAAGA CTTGGCGTAT 
CAGATGACCA TTCATGACTG GGACCTATTC AGTTGTGTGC ATGAGCTGGA ACTTATTTAC 
CACACATTCG GGCGACAGAA TTTCAAAAAG ACGACGGCAA ATTTGGACCT CTTCCTGAGA 
CGTTTTAATG AAATTCAGTT CTGGGTTGTG ACTGAGATTT GCCTGTGCCC TCAACTCAGC 
AAGCGCGTTC AACTTCTAAA GAAATTTATT AAAATTGCGG CCCACTGCAA GGAATATAAA 
AATTTGAACT CGTTCTTTGC TATTGTCATG GGGCTGAGTA ACGTGGCCGT AAGCCGTCTC 
TCACTGACAT GGGAGAAACT CCCAAGCAAG TTCAAAAAGA TCTATGCAGA ATTTGAAAAT 
TTAATGGATC CATCAAGGAA TCACAGGGCT TACAGGCTCA CTGTAGCTAA ACTGGACCCT 
CCAATCATTC CATTTACACC TCTGCTTATC AAAGATATGA CTTTTACCCA TGAGGGCAAC 
AAAACCTTCA TTGACAATTT GGTTAACTTT GAGAAAATGC GAATGATCGC AAACACCTTG 
AGAACAATAA GATACTGCCG AAGCGTCCCT TTCAATCCTG ATGCCTCTCT TGCCGGCAAA 
AATCACCAAG ACGTGCGGAA TTATGTGCGC CAGTTCAACG TGATTGACAA CCAGAGGACA 
CTGTCTCAGA TGTCCCACCG GCTGGAACCA CGCCGAGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_002934617.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934663.3 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa66856
Clone ID Related Accession (Same CDS sequence)	XM_002934617.4
Accession Version	XM_002934617.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934617.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTTCCATCAG AAAGAATACT CAGGGCTGGA 
AAGACTTTGC GTAATGCCAT CCTTACTCGG GCGCCCCACA TGATTAGGGA TCGAAAGTAT 
CATCTGAAAA CATACAGGCA GTGCTGTGTG GGAACTGAAC TTGTTGACTG GCTTATGCAG 
CAAAGCTCAT GCGTTCATTC TCGGACGCAA GCAGTGGGAA TGTGGCAGGT GTTGCTCGAA 
GATGGAGTCC TAAACCATGT GGACCAGGAA TATTACTTCC AAGACAAATA CCTGTTTTAT 
CGGTTTCTTG ACGATGAACA CGAGGATGCA CCGATGCCAA CAGAGGAGGA GAAGAAAGAG 
AGCGACGAGG AGCTGCAGGA TACGTTGCTC TTACTCTCGC AGATAGGACC CGATGCACAC 
ATGAGAATGA TTCTGAGAAA ACCACCGGGT CAGAGGAGTG CAGATGACTT AGAAATTATT 
TTTGAAGAAC TTATTCACAT TAAAGCCTTA TCTCACTTAT CAACAACAGT GAAAAGAGAA 
CTTGCTGGAT TCCTAATCTT TGAATCTCAT CCCAAAGCAG GAACTGTGTT ATTTAACCAA 
GGAGAAGAGG GCACTTCCTG GTACATCATC TTGAAAGGAT CTGTCAATGT TGTCATCTAT 
GGAAAGGGAG TTGTTTGTAC CCTGCATGAG GGAGATGACT TTGGGAAGCT TGCCCTTGTT 
AACGACGCCC CAAGGGCAGC ATCCATTGTT TTACGAGAAG ACAATTGTCA CTTCCTGCGT 
GTGGACAAGG AGGACTTCAA CCGGATCCTG CGTGACGTGG AGGCCAACAC AGTGAGACTC 
AAGGAGCACG ATCAAGATGT CTTGGTACTA GAAAAAATCC TGGCTGGATC TCAAGGCTCA 
GCACAAGGGA ACACTCAGTC GCCATATAAA TACACTGTGA TGTCAGGAAA CCCAGAGAAA 
CTTCTAGAAC ACTTCCTGGA AACAATGCGC CTTGATCCGA GTTTAAATGA TGCAACAGAC 
ACCGCTTTGG ACGACTTTAT TCTGATGCAC TGTGTTTTTA TGCCAAATTC TCAGCTCTGC 
CCGGCCTTGA TGTCACATTA CCATGCGCAG CCTTCCCAGG GAACGGAGCA GGAGAAGCTG 
GATTACACCC TGAATAACAA GCGCAGGGTC ATACGGCTGG TTCAGCAGTG GGCCACTCTG 
TATGGGGACC TGCTCCGTGA GGATGAGGTT TCAATGGCCT TTCTTGAAGA ATTTTATGTG 
TCGGTATCAG ATGACACAAG AACGACTGCA GCCCTGAAGG AGCAGCTACC TGACCTAGAA 
CGAGCCATCA AGCAAATATC TGAGGATGCA AAGGGGCAAC AAAAGAAGCA CAAGGTGCTT 
TTACGGCAAT TCAGCACTGG AGATGAGAGA CTGCAAAAGC GCCAGGCAAT ACGGAGTTCC 
GATGAACTTC TGTTCAAGGT ATACTGCATA GACCATACGT ACACCACTAT CCGTGTCGCT 
GTGGCTGCTT CAGTGAAAGA AGTGCTCTCT GCGGTCGCCG ATAAACTAGG CTCCGGGGAC 
AGCTTGATAC TCGTCAAAAT CAGTTCTGCA GGAGAAAAAG TGGTGCTGAA ACCAAATGAA 
ATATCCGTCT TTACAACGCT CAGCGTGAAT GGACGTCTGT TTGCCTGCCC CCGGGACCAG 
TTCGATTCTT TGACCCCTCT ACCAGAACAA GAGGGGCCTT CCGCTGGAAC CATGTCAACT 
TTTGAACTCA TGAGCTCTAA AGACTTGGCG TATCAGATGA CCATTCATGA CTGGGACCTA 
TTCAGTTGTG TGCATGAGCT GGAACTTATT TACCACACAT TCGGGCGACA GAATTTCAAA 
AAGACGACGG CAAATTTGGA CCTCTTCCTG AGACGTTTTA ATGAAATTCA GTTCTGGGTT 
GTGACTGAGA TTTGCCTGTG CCCTCAACTC AGCAAGCGCG TTCAACTTCT AAAGAAATTT 
ATTAAAATTG CGGCCCACTG CAAGGAATAT AAAAATTTGA ACTCGTTCTT TGCTATTGTC 
ATGGGGCTGA GTAACGTGGC CGTAAGCCGT CTCTCACTGA CATGGGAGAA ACTCCCAAGC 
AAGTTCAAAA AGATCTATGC AGAATTTGAA AATTTAATGG ATCCATCAAG GAATCACAGG 
GCTTACAGGC TCACTGTAGC TAAACTGGAC CCTCCAATCA TTCCATTTAC ACCTCTGCTT 
ATCAAAGATA TGACTTTTAC CCATGAGGGC AACAAAACCT TCATTGACAA TTTGGTTAAC 
TTTGAGAAAA TGCGAATGAT CGCAAACACC TTGAGAACAA TAAGATACTG CCGAAGCGTC 
CCTTTCAATC CTGATGCCTC TCTTGCCGGC AAAAATCACC AAGACGTGCG GAATTATGTG 
CGCCAGTTCA ACGTGATTGA CAACCAGAGG ACACTGTCTC AGATGTCCCA CCGGCTGGAA 
CCACGCCGAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934663.3
CDS	285..3359
Translation	MVAAHTAHSTSSAEWIACLDKRPLERSGEDVDIIFARLKEVKAFERFHPNLLQQICFCGYYENLEKGITLYRQGDIGTSWYAVLTGSLDVKVSETNNHQDAVVICTLGVGTAFGESILDNTPRHATIVTRENSELLRIEQKDFKTLWEKYRQYMAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKTPLIENQIPIRPAKTITEVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEDGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_002934617.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934617.4

ORF Insert Sequence:

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTTCCATCAG AAAGAATACT CAGGGCTGGA 
AAGACTTTGC GTAATGCCAT CCTTACTCGG GCGCCCCACA TGATTAGGGA TCGAAAGTAT 
CATCTGAAAA CATACAGGCA GTGCTGTGTG GGAACTGAAC TTGTTGACTG GCTTATGCAG 
CAAAGCTCAT GCGTTCATTC TCGGACGCAA GCAGTGGGAA TGTGGCAGGT GTTGCTCGAA 
GATGGAGTCC TAAACCATGT GGACCAGGAA TATTACTTCC AAGACAAATA CCTGTTTTAT 
CGGTTTCTTG ACGATGAACA CGAGGATGCA CCGATGCCAA CAGAGGAGGA GAAGAAAGAG 
AGCGACGAGG AGCTGCAGGA TACGTTGCTC TTACTCTCGC AGATAGGACC CGATGCACAC 
ATGAGAATGA TTCTGAGAAA ACCACCGGGT CAGAGGAGTG CAGATGACTT AGAAATTATT 
TTTGAAGAAC TTATTCACAT TAAAGCCTTA TCTCACTTAT CAACAACAGT GAAAAGAGAA 
CTTGCTGGAT TCCTAATCTT TGAATCTCAT CCCAAAGCAG GAACTGTGTT ATTTAACCAA 
GGAGAAGAGG GCACTTCCTG GTACATCATC TTGAAAGGAT CTGTCAATGT TGTCATCTAT 
GGAAAGGGAG TTGTTTGTAC CCTGCATGAG GGAGATGACT TTGGGAAGCT TGCCCTTGTT 
AACGACGCCC CAAGGGCAGC ATCCATTGTT TTACGAGAAG ACAATTGTCA CTTCCTGCGT 
GTGGACAAGG AGGACTTCAA CCGGATCCTG CGTGACGTGG AGGCCAACAC AGTGAGACTC 
AAGGAGCACG ATCAAGATGT CTTGGTACTA GAAAAAATCC TGGCTGGATC TCAAGGCTCA 
GCACAAGGGA ACACTCAGTC GCCATATAAA TACACTGTGA TGTCAGGAAA CCCAGAGAAA 
CTTCTAGAAC ACTTCCTGGA AACAATGCGC CTTGATCCGA GTTTAAATGA TGCAACAGAC 
ACCGCTTTGG ACGACTTTAT TCTGATGCAC TGTGTTTTTA TGCCAAATTC TCAGCTCTGC 
CCGGCCTTGA TGTCACATTA CCATGCGCAG CCTTCCCAGG GAACGGAGCA GGAGAAGCTG 
GATTACACCC TGAATAACAA GCGCAGGGTC ATACGGCTGG TTCAGCAGTG GGCCACTCTG 
TATGGGGACC TGCTCCGTGA GGATGAGGTT TCAATGGCCT TTCTTGAAGA ATTTTATGTG 
TCGGTATCAG ATGACACAAG AACGACTGCA GCCCTGAAGG AGCAGCTACC TGACCTAGAA 
CGAGCCATCA AGCAAATATC TGAGGATGCA AAGGGGCAAC AAAAGAAGCA CAAGGTGCTT 
TTACGGCAAT TCAGCACTGG AGATGAGAGA CTGCAAAAGC GCCAGGCAAT ACGGAGTTCC 
GATGAACTTC TGTTCAAGGT ATACTGCATA GACCATACGT ACACCACTAT CCGTGTCGCT 
GTGGCTGCTT CAGTGAAAGA AGTGCTCTCT GCGGTCGCCG ATAAACTAGG CTCCGGGGAC 
AGCTTGATAC TCGTCAAAAT CAGTTCTGCA GGAGAAAAAG TGGTGCTGAA ACCAAATGAA 
ATATCCGTCT TTACAACGCT CAGCGTGAAT GGACGTCTGT TTGCCTGCCC CCGGGACCAG 
TTCGATTCTT TGACCCCTCT ACCAGAACAA GAGGGGCCTT CCGCTGGAAC CATGTCAACT 
TTTGAACTCA TGAGCTCTAA AGACTTGGCG TATCAGATGA CCATTCATGA CTGGGACCTA 
TTCAGTTGTG TGCATGAGCT GGAACTTATT TACCACACAT TCGGGCGACA GAATTTCAAA 
AAGACGACGG CAAATTTGGA CCTCTTCCTG AGACGTTTTA ATGAAATTCA GTTCTGGGTT 
GTGACTGAGA TTTGCCTGTG CCCTCAACTC AGCAAGCGCG TTCAACTTCT AAAGAAATTT 
ATTAAAATTG CGGCCCACTG CAAGGAATAT AAAAATTTGA ACTCGTTCTT TGCTATTGTC 
ATGGGGCTGA GTAACGTGGC CGTAAGCCGT CTCTCACTGA CATGGGAGAA ACTCCCAAGC 
AAGTTCAAAA AGATCTATGC AGAATTTGAA AATTTAATGG ATCCATCAAG GAATCACAGG 
GCTTACAGGC TCACTGTAGC TAAACTGGAC CCTCCAATCA TTCCATTTAC ACCTCTGCTT 
ATCAAAGATA TGACTTTTAC CCATGAGGGC AACAAAACCT TCATTGACAA TTTGGTTAAC 
TTTGAGAAAA TGCGAATGAT CGCAAACACC TTGAGAACAA TAAGATACTG CCGAAGCGTC 
CCTTTCAATC CTGATGCCTC TCTTGCCGGC AAAAATCACC AAGACGTGCG GAATTATGTG 
CGCCAGTTCA ACGTGATTGA CAACCAGAGG ACACTGTCTC AGATGTCCCA CCGGCTGGAA 
CCACGCCGAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_004917715.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917772.2 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa66857
Clone ID Related Accession (Same CDS sequence)	XM_004917715.3
Accession Version	XM_004917715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGGTTC CATCAGAAAG AATACTCAGG 
GCTGGAAAGA CTTTGCGTAA TGCCATCCTT ACTCGGGCGC CCCACATGAT TAGGGATCGA 
AAGTATCATC TGAAAACATA CAGGCAGTGC TGTGTGGGAA CTGAACTTGT TGACTGGCTT 
ATGCAGCAAA GCTCATGCGT TCATTCTCGG ACGCAAGCAG TGGGAATGTG GCAGGTGTTG 
CTCGAAGATG GAGTCCTAAA CCATGTGGAC CAGGAATATT ACTTCCAAGA CAAATACCTG 
TTTTATCGGT TTCTTGACGA TGAACACGAG GATGCACCGA TGCCAACAGA GGAGGAGAAG 
AAAGAGAGCG ACGAGGAGCT GCAGGATACG TTGCTCTTAC TCTCGCAGAT AGGACCCGAT 
GCACACATGA GAATGATTCT GAGAAAACCA CCGGGTCAGA GGAGTGCAGA TGACTTAGAA 
ATTATTTTTG AAGAACTTAT TCACATTAAA GCCTTATCTC ACTTATCAAC AACAGTGAAA 
AGAGAACTTG CTGGATTCCT AATCTTTGAA TCTCATCCCA AAGCAGGAAC TGTGTTATTT 
AACCAAGGAG AAGAGGGCAC TTCCTGGTAC ATCATCTTGA AAGGATCTGT CAATGTTGTC 
ATCTATGGAA AGGGAGTTGT TTGTACCCTG CATGAGGGAG ATGACTTTGG GAAGCTTGCC 
CTTGTTAACG ACGCCCCAAG GGCAGCATCC ATTGTTTTAC GAGAAGACAA TTGTCACTTC 
CTGCGTGTGG ACAAGGAGGA CTTCAACCGG ATCCTGCGTG ACGTGGAGGC CAACACAGTG 
AGACTCAAGG AGCACGATCA AGATGTCTTG GTACTAGAAA AAATCCTGGC TGGATCTCAA 
GGCTCAGCAC AAGGGAACAC TCAGTCGCCA TATAAATACA CTGTGATGTC AGGAAACCCA 
GAGAAACTTC TAGAACACTT CCTGGAAACA ATGCGCCTTG ATCCGAGTTT AAATGATGCA 
ACAGACACCG CTTTGGACGA CTTTATTCTG ATGCACTGTG TTTTTATGCC AAATTCTCAG 
CTCTGCCCGG CCTTGATGTC ACATTACCAT GCGCAGCCTT CCCAGGGAAC GGAGCAGGAG 
AAGCTGGATT ACACCCTGAA TAACAAGCGC AGGGTCATAC GGCTGGTTCA GCAGTGGGCC 
ACTCTGTATG GGGACCTGCT CCGTGAGGAT GAGGTTTCAA TGGCCTTTCT TGAAGAATTT 
TATGTGTCGG TATCAGATGA CACAAGAACG ACTGCAGCCC TGAAGGAGCA GCTACCTGAC 
CTAGAACGAG CCATCAAGCA AATATCTGAG GATGCAAAGG GGCAACAAAA GAAGCACAAG 
GTGCTTTTAC GGCAATTCAG CACTGGAGAT GAGAGACTGC AAAAGCGCCA GGCAATACGG 
AGTTCCGATG AACTTCTGTT CAAGGTATAC TGCATAGACC ATACGTACAC CACTATCCGT 
GTCGCTGTGG CTGCTTCAGT GAAAGAAGTG CTCTCTGCGG TCGCCGATAA ACTAGGCTCC 
GGGGACAGCT TGATACTCGT CAAAATCAGT TCTGCAGGAG AAAAAGTGGT GCTGAAACCA 
AATGAAATAT CCGTCTTTAC AACGCTCAGC GTGAATGGAC GTCTGTTTGC CTGCCCCCGG 
GACCAGTTCG ATTCTTTGAC CCCTCTACCA GAACAAGAGG GGCCTTCCGC TGGAACCATG 
TCAACTTTTG AACTCATGAG CTCTAAAGAC TTGGCGTATC AGATGACCAT TCATGACTGG 
GACCTATTCA GTTGTGTGCA TGAGCTGGAA CTTATTTACC ACACATTCGG GCGACAGAAT 
TTCAAAAAGA CGACGGCAAA TTTGGACCTC TTCCTGAGAC GTTTTAATGA AATTCAGTTC 
TGGGTTGTGA CTGAGATTTG CCTGTGCCCT CAACTCAGCA AGCGCGTTCA ACTTCTAAAG 
AAATTTATTA AAATTGCGGC CCACTGCAAG GAATATAAAA ATTTGAACTC GTTCTTTGCT 
ATTGTCATGG GGCTGAGTAA CGTGGCCGTA AGCCGTCTCT CACTGACATG GGAGAAACTC 
CCAAGCAAGT TCAAAAAGAT CTATGCAGAA TTTGAAAATT TAATGGATCC ATCAAGGAAT 
CACAGGGCTT ACAGGCTCAC TGTAGCTAAA CTGGACCCTC CAATCATTCC ATTTACACCT 
CTGCTTATCA AAGATATGAC TTTTACCCAT GAGGGCAACA AAACCTTCAT TGACAATTTG 
GTTAACTTTG AGAAAATGCG AATGATCGCA AACACCTTGA GAACAATAAG ATACTGCCGA 
AGCGTCCCTT TCAATCCTGA TGCCTCTCTT GCCGGCAAAA ATCACCAAGA CGTGCGGAAT 
TATGTGCGCC AGTTCAACGT GATTGACAAC CAGAGGACAC TGTCTCAGAT GTCCCACCGG 
CTGGAACCAC GCCGAGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917772.2
CDS	285..3305
Translation	MVAAHTAHSTSSAEWIACLDKRPLERSGEDVDIIFARLKEVKAFERFHPNLLQQICFCGYYENLEKGITLYRQGDIGTSWYAVLTGSLDVKVSETNNHQDAVVICTLGVGTAFGESILDNTPRHATIVTRENSELLRIEQKDFKTLWEKYRQYMAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEDGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_004917715.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917715.3

ORF Insert Sequence:

ATGGTGGCTG CACACACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CCTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGGTTC CATCAGAAAG AATACTCAGG 
GCTGGAAAGA CTTTGCGTAA TGCCATCCTT ACTCGGGCGC CCCACATGAT TAGGGATCGA 
AAGTATCATC TGAAAACATA CAGGCAGTGC TGTGTGGGAA CTGAACTTGT TGACTGGCTT 
ATGCAGCAAA GCTCATGCGT TCATTCTCGG ACGCAAGCAG TGGGAATGTG GCAGGTGTTG 
CTCGAAGATG GAGTCCTAAA CCATGTGGAC CAGGAATATT ACTTCCAAGA CAAATACCTG 
TTTTATCGGT TTCTTGACGA TGAACACGAG GATGCACCGA TGCCAACAGA GGAGGAGAAG 
AAAGAGAGCG ACGAGGAGCT GCAGGATACG TTGCTCTTAC TCTCGCAGAT AGGACCCGAT 
GCACACATGA GAATGATTCT GAGAAAACCA CCGGGTCAGA GGAGTGCAGA TGACTTAGAA 
ATTATTTTTG AAGAACTTAT TCACATTAAA GCCTTATCTC ACTTATCAAC AACAGTGAAA 
AGAGAACTTG CTGGATTCCT AATCTTTGAA TCTCATCCCA AAGCAGGAAC TGTGTTATTT 
AACCAAGGAG AAGAGGGCAC TTCCTGGTAC ATCATCTTGA AAGGATCTGT CAATGTTGTC 
ATCTATGGAA AGGGAGTTGT TTGTACCCTG CATGAGGGAG ATGACTTTGG GAAGCTTGCC 
CTTGTTAACG ACGCCCCAAG GGCAGCATCC ATTGTTTTAC GAGAAGACAA TTGTCACTTC 
CTGCGTGTGG ACAAGGAGGA CTTCAACCGG ATCCTGCGTG ACGTGGAGGC CAACACAGTG 
AGACTCAAGG AGCACGATCA AGATGTCTTG GTACTAGAAA AAATCCTGGC TGGATCTCAA 
GGCTCAGCAC AAGGGAACAC TCAGTCGCCA TATAAATACA CTGTGATGTC AGGAAACCCA 
GAGAAACTTC TAGAACACTT CCTGGAAACA ATGCGCCTTG ATCCGAGTTT AAATGATGCA 
ACAGACACCG CTTTGGACGA CTTTATTCTG ATGCACTGTG TTTTTATGCC AAATTCTCAG 
CTCTGCCCGG CCTTGATGTC ACATTACCAT GCGCAGCCTT CCCAGGGAAC GGAGCAGGAG 
AAGCTGGATT ACACCCTGAA TAACAAGCGC AGGGTCATAC GGCTGGTTCA GCAGTGGGCC 
ACTCTGTATG GGGACCTGCT CCGTGAGGAT GAGGTTTCAA TGGCCTTTCT TGAAGAATTT 
TATGTGTCGG TATCAGATGA CACAAGAACG ACTGCAGCCC TGAAGGAGCA GCTACCTGAC 
CTAGAACGAG CCATCAAGCA AATATCTGAG GATGCAAAGG GGCAACAAAA GAAGCACAAG 
GTGCTTTTAC GGCAATTCAG CACTGGAGAT GAGAGACTGC AAAAGCGCCA GGCAATACGG 
AGTTCCGATG AACTTCTGTT CAAGGTATAC TGCATAGACC ATACGTACAC CACTATCCGT 
GTCGCTGTGG CTGCTTCAGT GAAAGAAGTG CTCTCTGCGG TCGCCGATAA ACTAGGCTCC 
GGGGACAGCT TGATACTCGT CAAAATCAGT TCTGCAGGAG AAAAAGTGGT GCTGAAACCA 
AATGAAATAT CCGTCTTTAC AACGCTCAGC GTGAATGGAC GTCTGTTTGC CTGCCCCCGG 
GACCAGTTCG ATTCTTTGAC CCCTCTACCA GAACAAGAGG GGCCTTCCGC TGGAACCATG 
TCAACTTTTG AACTCATGAG CTCTAAAGAC TTGGCGTATC AGATGACCAT TCATGACTGG 
GACCTATTCA GTTGTGTGCA TGAGCTGGAA CTTATTTACC ACACATTCGG GCGACAGAAT 
TTCAAAAAGA CGACGGCAAA TTTGGACCTC TTCCTGAGAC GTTTTAATGA AATTCAGTTC 
TGGGTTGTGA CTGAGATTTG CCTGTGCCCT CAACTCAGCA AGCGCGTTCA ACTTCTAAAG 
AAATTTATTA AAATTGCGGC CCACTGCAAG GAATATAAAA ATTTGAACTC GTTCTTTGCT 
ATTGTCATGG GGCTGAGTAA CGTGGCCGTA AGCCGTCTCT CACTGACATG GGAGAAACTC 
CCAAGCAAGT TCAAAAAGAT CTATGCAGAA TTTGAAAATT TAATGGATCC ATCAAGGAAT 
CACAGGGCTT ACAGGCTCAC TGTAGCTAAA CTGGACCCTC CAATCATTCC ATTTACACCT 
CTGCTTATCA AAGATATGAC TTTTACCCAT GAGGGCAACA AAACCTTCAT TGACAATTTG 
GTTAACTTTG AGAAAATGCG AATGATCGCA AACACCTTGA GAACAATAAG ATACTGCCGA 
AGCGTCCCTT TCAATCCTGA TGCCTCTCTT GCCGGCAAAA ATCACCAAGA CGTGCGGAAT 
TATGTGCGCC AGTTCAACGT GATTGACAAC CAGAGGACAC TGTCTCAGAT GTCCCACCGG 
CTGGAACCAC GCCGAGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_002934617.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934663.2 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa15917
Clone ID Related Accession (Same CDS sequence)	XM_002934617.3
Accession Version	XM_002934617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002934617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTG CACATACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CTTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTTCCATCAG AAAGAATACT CAGGGCTGGA 
AAGACTTTGC GTAATGCCAT CCTTACTCGG GCGCCCCACA TGATTAGGGA TCGAAAGTAT 
CATCTGAAAA CATACAGGCA GTGCTGTGTG GGAACTGAAC TTGTTGACTG GCTTATGCAG 
CAAAGCTCAT GCGTTCATTC TCGGACGCAA GCAGTGGGAA TGTGGCAGGT GTTGCTCGAA 
GAAGGAGTCC TAAACCATGT GGACCAGGAA TATTACTTCC AAGACAAATA CCTGTTTTAT 
CGGTTTCTTG ATGATGAACA CGAGGATGCA CCGATGCCAA CAGAGGAGGA GAAGAAAGAG 
AGCGACGAGG AGCTGCAGGA TACGTTGCTC TTACTCTCGC AGATAGGACC CGATGCACAC 
ATGAGAATGA TTCTGAGAAA ACCACCGGGT CAGAGGAGTG CAGATGACTT AGAAATTATT 
TTTGAAGAAC TTATTCACAT TAAAGCCTTA TCTCACTTAT CAACAACAGT GAAAAGAGAA 
CTTGCTGGAT TCCTAATCTT TGAATCTCAT CCCAAAGCAG GAACTGTGTT ATTTAACCAA 
GGAGAAGAGG GCACTTCCTG GTACATCATC TTGAAAGGAT CTGTCAATGT TGTCATCTAT 
GGAAAGGGAG TTGTTTGTAC CCTGCATGAG GGAGATGACT TTGGGAAGCT TGCCCTTGTT 
AACGACGCCC CAAGGGCAGC ATCCATTGTT TTACGAGAAG ACAATTGTCA CTTCCTGCGT 
GTGGACAAGG AGGACTTCAA CCGGATCCTG CGTGACGTGG AGGCCAACAC AGTGAGACTC 
AAGGAGCACG ATCAAGATGT CTTGGTACTA GAAAAAATCC TGGCTGGATC TCAAGGCTCA 
GCACAAGGGA ACACTCAGTC GCCATATAAA TACACTGTGA TGTCAGGAAA CCCAGAGAAA 
CTTCTAGAAC ACTTCCTGGA AACAATGCGC CTTGATCCGA GTTTAAATGA TGCAACAGAC 
ACCGCTTTGG ACGACTTTAT TCTGATGCAC TGTGTTTTTA TGCCAAATTC TCAGCTCTGC 
CCGGCCTTGA TGTCACATTA CCATGCGCAG CCTTCCCAGG GAACGGAGCA GGAGAAGCTG 
GATTACACCC TGAATAACAA GCGCAGGGTC ATACGGCTGG TTCAGCAGTG GGCCACTCTG 
TATGGGGACC TGCTCCGTGA GGATGAGGTT TCAATGGCCT TTCTTGAAGA ATTTTATGTG 
TCGGTATCAG ATGACACAAG AACGACTGCA GCCCTGAAGG AGCAGCTACC TGACCTAGAA 
CGAGCCATCA AGCAAATATC TGAGGATGCA AAGGGGCAAC AAAAGAAGCA CAAGGTGCTT 
TTACGGCAGT TCAGCACTGG AGATGAGAGA CTGCAAAAGC GCCAGGCAAT ACGGAGTTCC 
GATGAACTTC TGTTCAAGGT ATACTGCATA GACCATACGT ACACCACTAT CCGTGTCGCT 
GTGGCTGCTT CAGTGAAAGA AGTGCTCTCT GCGGTCGCCG ATAAACTAGG CTCCGGGGAC 
AGCTTGATAC TCGTCAAAAT CAGTTCTGCA GGAGAAAAAG TGGTGCTGAA ACCAAATGAA 
ATATCCGTCT TTACAACGCT CAGCGTGAAT GGACGTCTGT TTGCCTGCCC CCGGGACCAG 
TTCGATTCTT TGACCCCTCT ACCAGAACAA GAGGGGCCTT CCGCTGGAAC CATGTCAACT 
TTTGAACTCA TGAGCTCTAA AGACTTGGCG TATCAGATGA CCATTCATGA CTGGGACCTA 
TTCAGTTGTG TGCATGAGCT GGAACTTATT TACCACACAT TCGGGCGACA GAATTTCAAA 
AAGACGACGG CAAATTTGGA CCTCTTCCTG AGACGTTTTA ATGAAATTCA GTTCTGGGTT 
GTGACTGAGA TTTGCCTGTG CCCTCAACTC AGCAAGCGCG TTCAACTTCT AAAGAAATTT 
ATTAAAATTG CGGCCCACTG CAAGGAATAT AAAAATTTGA ACTCGTTCTT TGCTATTGTC 
ATGGGGCTGA GTAACGTGGC CGTAAGCCGT CTCTCACTGA CATGGGAGAA ACTCCCAAGC 
AAGTTCAAAA AGATCTATGC AGAATTTGAA AATTTAATGG ATCCATCAAG GAATCACAGG 
GCTTACAGGC TCACTGTAGC TAAACTGGAC CCTCCAATCA TTCCATTTAC ACCTCTGCTT 
ATCAAAGATA TGACTTTTAC CCATGAGGGC AACAAAACCT TCATTGACAA TTTGGTTAAC 
TTTGAGAAAA TGCGAATGAT CGCAAACACC TTGAGAACAA TAAGATACTG CCGAAGCGTC 
CCTTTCAATC CTGATGCCTC TCTTGCCGGC AAAAATCACC AAGACGTGCG GAATTATGTG 
CGCCAGTTCA ACGTGATTGA CAACCAGAGG ACACTGTCTC AGATGTCCCA CCGGCTGGAA 
CCACGCCGAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934663.2
CDS	262..3336
Translation	MVAAHTAHSTSSAEWIACLDKRPLERSGEDVDIIFARLKEVKAFERFHPNLLQQICFCGYYENLEKGITLYRQGDIGTSWYAVLTGSLDVKVSETNNHQDAVVICTLGVGTAFGESILDNTPRHATIVTRENSELLRIEQKDFKTLWEKYRQYMAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKTPLIENQIPIRPAKTITEVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEEGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_002934617.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934617.3

ORF Insert Sequence:

ATGGTGGCTG CACATACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CTTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGACCC CACTCATTGA AAATCAAATC 
CCGATACGTC CAGCTAAAAC CATTACAGAG GTTCCATCAG AAAGAATACT CAGGGCTGGA 
AAGACTTTGC GTAATGCCAT CCTTACTCGG GCGCCCCACA TGATTAGGGA TCGAAAGTAT 
CATCTGAAAA CATACAGGCA GTGCTGTGTG GGAACTGAAC TTGTTGACTG GCTTATGCAG 
CAAAGCTCAT GCGTTCATTC TCGGACGCAA GCAGTGGGAA TGTGGCAGGT GTTGCTCGAA 
GAAGGAGTCC TAAACCATGT GGACCAGGAA TATTACTTCC AAGACAAATA CCTGTTTTAT 
CGGTTTCTTG ATGATGAACA CGAGGATGCA CCGATGCCAA CAGAGGAGGA GAAGAAAGAG 
AGCGACGAGG AGCTGCAGGA TACGTTGCTC TTACTCTCGC AGATAGGACC CGATGCACAC 
ATGAGAATGA TTCTGAGAAA ACCACCGGGT CAGAGGAGTG CAGATGACTT AGAAATTATT 
TTTGAAGAAC TTATTCACAT TAAAGCCTTA TCTCACTTAT CAACAACAGT GAAAAGAGAA 
CTTGCTGGAT TCCTAATCTT TGAATCTCAT CCCAAAGCAG GAACTGTGTT ATTTAACCAA 
GGAGAAGAGG GCACTTCCTG GTACATCATC TTGAAAGGAT CTGTCAATGT TGTCATCTAT 
GGAAAGGGAG TTGTTTGTAC CCTGCATGAG GGAGATGACT TTGGGAAGCT TGCCCTTGTT 
AACGACGCCC CAAGGGCAGC ATCCATTGTT TTACGAGAAG ACAATTGTCA CTTCCTGCGT 
GTGGACAAGG AGGACTTCAA CCGGATCCTG CGTGACGTGG AGGCCAACAC AGTGAGACTC 
AAGGAGCACG ATCAAGATGT CTTGGTACTA GAAAAAATCC TGGCTGGATC TCAAGGCTCA 
GCACAAGGGA ACACTCAGTC GCCATATAAA TACACTGTGA TGTCAGGAAA CCCAGAGAAA 
CTTCTAGAAC ACTTCCTGGA AACAATGCGC CTTGATCCGA GTTTAAATGA TGCAACAGAC 
ACCGCTTTGG ACGACTTTAT TCTGATGCAC TGTGTTTTTA TGCCAAATTC TCAGCTCTGC 
CCGGCCTTGA TGTCACATTA CCATGCGCAG CCTTCCCAGG GAACGGAGCA GGAGAAGCTG 
GATTACACCC TGAATAACAA GCGCAGGGTC ATACGGCTGG TTCAGCAGTG GGCCACTCTG 
TATGGGGACC TGCTCCGTGA GGATGAGGTT TCAATGGCCT TTCTTGAAGA ATTTTATGTG 
TCGGTATCAG ATGACACAAG AACGACTGCA GCCCTGAAGG AGCAGCTACC TGACCTAGAA 
CGAGCCATCA AGCAAATATC TGAGGATGCA AAGGGGCAAC AAAAGAAGCA CAAGGTGCTT 
TTACGGCAGT TCAGCACTGG AGATGAGAGA CTGCAAAAGC GCCAGGCAAT ACGGAGTTCC 
GATGAACTTC TGTTCAAGGT ATACTGCATA GACCATACGT ACACCACTAT CCGTGTCGCT 
GTGGCTGCTT CAGTGAAAGA AGTGCTCTCT GCGGTCGCCG ATAAACTAGG CTCCGGGGAC 
AGCTTGATAC TCGTCAAAAT CAGTTCTGCA GGAGAAAAAG TGGTGCTGAA ACCAAATGAA 
ATATCCGTCT TTACAACGCT CAGCGTGAAT GGACGTCTGT TTGCCTGCCC CCGGGACCAG 
TTCGATTCTT TGACCCCTCT ACCAGAACAA GAGGGGCCTT CCGCTGGAAC CATGTCAACT 
TTTGAACTCA TGAGCTCTAA AGACTTGGCG TATCAGATGA CCATTCATGA CTGGGACCTA 
TTCAGTTGTG TGCATGAGCT GGAACTTATT TACCACACAT TCGGGCGACA GAATTTCAAA 
AAGACGACGG CAAATTTGGA CCTCTTCCTG AGACGTTTTA ATGAAATTCA GTTCTGGGTT 
GTGACTGAGA TTTGCCTGTG CCCTCAACTC AGCAAGCGCG TTCAACTTCT AAAGAAATTT 
ATTAAAATTG CGGCCCACTG CAAGGAATAT AAAAATTTGA ACTCGTTCTT TGCTATTGTC 
ATGGGGCTGA GTAACGTGGC CGTAAGCCGT CTCTCACTGA CATGGGAGAA ACTCCCAAGC 
AAGTTCAAAA AGATCTATGC AGAATTTGAA AATTTAATGG ATCCATCAAG GAATCACAGG 
GCTTACAGGC TCACTGTAGC TAAACTGGAC CCTCCAATCA TTCCATTTAC ACCTCTGCTT 
ATCAAAGATA TGACTTTTAC CCATGAGGGC AACAAAACCT TCATTGACAA TTTGGTTAAC 
TTTGAGAAAA TGCGAATGAT CGCAAACACC TTGAGAACAA TAAGATACTG CCGAAGCGTC 
CCTTTCAATC CTGATGCCTC TCTTGCCGGC AAAAATCACC AAGACGTGCG GAATTATGTG 
CGCCAGTTCA ACGTGATTGA CAACCAGAGG ACACTGTCTC AGATGTCCCA CCGGCTGGAA 
CCACGCCGAG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef4 ( XM_004917715.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917772.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.

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CloneID	OXa15918
Clone ID Related Accession (Same CDS sequence)	XM_004917715.2
Accession Version	XM_004917715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004917715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCTG CACATACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CTTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGGTTC CATCAGAAAG AATACTCAGG 
GCTGGAAAGA CTTTGCGTAA TGCCATCCTT ACTCGGGCGC CCCACATGAT TAGGGATCGA 
AAGTATCATC TGAAAACATA CAGGCAGTGC TGTGTGGGAA CTGAACTTGT TGACTGGCTT 
ATGCAGCAAA GCTCATGCGT TCATTCTCGG ACGCAAGCAG TGGGAATGTG GCAGGTGTTG 
CTCGAAGAAG GAGTCCTAAA CCATGTGGAC CAGGAATATT ACTTCCAAGA CAAATACCTG 
TTTTATCGGT TTCTTGATGA TGAACACGAG GATGCACCGA TGCCAACAGA GGAGGAGAAG 
AAAGAGAGCG ACGAGGAGCT GCAGGATACG TTGCTCTTAC TCTCGCAGAT AGGACCCGAT 
GCACACATGA GAATGATTCT GAGAAAACCA CCGGGTCAGA GGAGTGCAGA TGACTTAGAA 
ATTATTTTTG AAGAACTTAT TCACATTAAA GCCTTATCTC ACTTATCAAC AACAGTGAAA 
AGAGAACTTG CTGGATTCCT AATCTTTGAA TCTCATCCCA AAGCAGGAAC TGTGTTATTT 
AACCAAGGAG AAGAGGGCAC TTCCTGGTAC ATCATCTTGA AAGGATCTGT CAATGTTGTC 
ATCTATGGAA AGGGAGTTGT TTGTACCCTG CATGAGGGAG ATGACTTTGG GAAGCTTGCC 
CTTGTTAACG ACGCCCCAAG GGCAGCATCC ATTGTTTTAC GAGAAGACAA TTGTCACTTC 
CTGCGTGTGG ACAAGGAGGA CTTCAACCGG ATCCTGCGTG ACGTGGAGGC CAACACAGTG 
AGACTCAAGG AGCACGATCA AGATGTCTTG GTACTAGAAA AAATCCTGGC TGGATCTCAA 
GGCTCAGCAC AAGGGAACAC TCAGTCGCCA TATAAATACA CTGTGATGTC AGGAAACCCA 
GAGAAACTTC TAGAACACTT CCTGGAAACA ATGCGCCTTG ATCCGAGTTT AAATGATGCA 
ACAGACACCG CTTTGGACGA CTTTATTCTG ATGCACTGTG TTTTTATGCC AAATTCTCAG 
CTCTGCCCGG CCTTGATGTC ACATTACCAT GCGCAGCCTT CCCAGGGAAC GGAGCAGGAG 
AAGCTGGATT ACACCCTGAA TAACAAGCGC AGGGTCATAC GGCTGGTTCA GCAGTGGGCC 
ACTCTGTATG GGGACCTGCT CCGTGAGGAT GAGGTTTCAA TGGCCTTTCT TGAAGAATTT 
TATGTGTCGG TATCAGATGA CACAAGAACG ACTGCAGCCC TGAAGGAGCA GCTACCTGAC 
CTAGAACGAG CCATCAAGCA AATATCTGAG GATGCAAAGG GGCAACAAAA GAAGCACAAG 
GTGCTTTTAC GGCAGTTCAG CACTGGAGAT GAGAGACTGC AAAAGCGCCA GGCAATACGG 
AGTTCCGATG AACTTCTGTT CAAGGTATAC TGCATAGACC ATACGTACAC CACTATCCGT 
GTCGCTGTGG CTGCTTCAGT GAAAGAAGTG CTCTCTGCGG TCGCCGATAA ACTAGGCTCC 
GGGGACAGCT TGATACTCGT CAAAATCAGT TCTGCAGGAG AAAAAGTGGT GCTGAAACCA 
AATGAAATAT CCGTCTTTAC AACGCTCAGC GTGAATGGAC GTCTGTTTGC CTGCCCCCGG 
GACCAGTTCG ATTCTTTGAC CCCTCTACCA GAACAAGAGG GGCCTTCCGC TGGAACCATG 
TCAACTTTTG AACTCATGAG CTCTAAAGAC TTGGCGTATC AGATGACCAT TCATGACTGG 
GACCTATTCA GTTGTGTGCA TGAGCTGGAA CTTATTTACC ACACATTCGG GCGACAGAAT 
TTCAAAAAGA CGACGGCAAA TTTGGACCTC TTCCTGAGAC GTTTTAATGA AATTCAGTTC 
TGGGTTGTGA CTGAGATTTG CCTGTGCCCT CAACTCAGCA AGCGCGTTCA ACTTCTAAAG 
AAATTTATTA AAATTGCGGC CCACTGCAAG GAATATAAAA ATTTGAACTC GTTCTTTGCT 
ATTGTCATGG GGCTGAGTAA CGTGGCCGTA AGCCGTCTCT CACTGACATG GGAGAAACTC 
CCAAGCAAGT TCAAAAAGAT CTATGCAGAA TTTGAAAATT TAATGGATCC ATCAAGGAAT 
CACAGGGCTT ACAGGCTCAC TGTAGCTAAA CTGGACCCTC CAATCATTCC ATTTACACCT 
CTGCTTATCA AAGATATGAC TTTTACCCAT GAGGGCAACA AAACCTTCAT TGACAATTTG 
GTTAACTTTG AGAAAATGCG AATGATCGCA AACACCTTGA GAACAATAAG ATACTGCCGA 
AGCGTCCCTT TCAATCCTGA TGCCTCTCTT GCCGGCAAAA ATCACCAAGA CGTGCGGAAT 
TATGTGCGCC AGTTCAACGT GATTGACAAC CAGAGGACAC TGTCTCAGAT GTCCCACCGG 
CTGGAACCAC GCCGAGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917772.1
CDS	262..3282
Translation	MVAAHTAHSTSSAEWIACLDKRPLERSGEDVDIIFARLKEVKAFERFHPNLLQQICFCGYYENLEKGITLYRQGDIGTSWYAVLTGSLDVKVSETNNHQDAVVICTLGVGTAFGESILDNTPRHATIVTRENSELLRIEQKDFKTLWEKYRQYMAGFLAPPYGVLETGSNDRLSDKENMNSALGPGSKNCNKVPSERILRAGKTLRNAILTRAPHMIRDRKYHLKTYRQCCVGTELVDWLMQQSSCVHSRTQAVGMWQVLLEEGVLNHVDQEYYFQDKYLFYRFLDDEHEDAPMPTEEEKKESDEELQDTLLLLSQIGPDAHMRMILRKPPGQRSADDLEIIFEELIHIKALSHLSTTVKRELAGFLIFESHPKAGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKILAGSQGSAQGNTQSPYKYTVMSGNPEKLLEHFLETMRLDPSLNDATDTALDDFILMHCVFMPNSQLCPALMSHYHAQPSQGTEQEKLDYTLNNKRRVIRLVQQWATLYGDLLREDEVSMAFLEEFYVSVSDDTRTTAALKEQLPDLERAIKQISEDAKGQQKKHKVLLRQFSTGDERLQKRQAIRSSDELLFKVYCIDHTYTTIRVAVAASVKEVLSAVADKLGSGDSLILVKISSAGEKVVLKPNEISVFTTLSVNGRLFACPRDQFDSLTPLPEQEGPSAGTMSTFELMSSKDLAYQMTIHDWDLFSCVHELELIYHTFGRQNFKKTTANLDLFLRRFNEIQFWVVTEICLCPQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLSLTWEKLPSKFKKIYAEFENLMDPSRNHRAYRLTVAKLDPPIIPFTPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTLRTIRYCRSVPFNPDASLAGKNHQDVRNYVRQFNVIDNQRTLSQMSHRLEPRRA

Target ORF information:

RefSeq Version	XM_004917715.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 4 (rapgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917715.2

ORF Insert Sequence:

ATGGTGGCTG CACATACTGC CCATTCTACC TCCTCTGCTG AATGGATTGC CTGCTTAGAT 
AAAAGGCCCC TGGAGCGTTC GGGAGAAGAT GTGGACATTA TATTTGCACG ACTGAAAGAA 
GTTAAAGCTT TTGAGCGATT TCACCCAAAT CTCCTTCAGC AGATCTGCTT TTGTGGATAC 
TATGAAAACC TGGAAAAGGG GATTACGTTG TACCGTCAAG GTGATATAGG AACAAGTTGG 
TATGCTGTGC TAACAGGATC ACTGGATGTT AAAGTATCTG AAACTAATAA CCACCAGGAT 
GCAGTCGTCA TTTGCACTTT GGGGGTGGGA ACGGCTTTTG GCGAATCCAT ATTGGACAAT 
ACCCCTCGGC ATGCCACCAT CGTCACAAGG GAAAATAGCG AACTCCTTCG CATTGAGCAG 
AAGGATTTTA AAACCCTTTG GGAGAAATAT CGGCAGTATA TGGCTGGCTT CTTGGCTCCA 
CCCTACGGTG TTCTGGAAAC CGGATCTAAT GACAGGCTCT CAGACAAAGA AAATATGAAT 
AGCGCTCTGG GACCAGGATC CAAAAACTGT AATAAGGTTC CATCAGAAAG AATACTCAGG 
GCTGGAAAGA CTTTGCGTAA TGCCATCCTT ACTCGGGCGC CCCACATGAT TAGGGATCGA 
AAGTATCATC TGAAAACATA CAGGCAGTGC TGTGTGGGAA CTGAACTTGT TGACTGGCTT 
ATGCAGCAAA GCTCATGCGT TCATTCTCGG ACGCAAGCAG TGGGAATGTG GCAGGTGTTG 
CTCGAAGAAG GAGTCCTAAA CCATGTGGAC CAGGAATATT ACTTCCAAGA CAAATACCTG 
TTTTATCGGT TTCTTGATGA TGAACACGAG GATGCACCGA TGCCAACAGA GGAGGAGAAG 
AAAGAGAGCG ACGAGGAGCT GCAGGATACG TTGCTCTTAC TCTCGCAGAT AGGACCCGAT 
GCACACATGA GAATGATTCT GAGAAAACCA CCGGGTCAGA GGAGTGCAGA TGACTTAGAA 
ATTATTTTTG AAGAACTTAT TCACATTAAA GCCTTATCTC ACTTATCAAC AACAGTGAAA 
AGAGAACTTG CTGGATTCCT AATCTTTGAA TCTCATCCCA AAGCAGGAAC TGTGTTATTT 
AACCAAGGAG AAGAGGGCAC TTCCTGGTAC ATCATCTTGA AAGGATCTGT CAATGTTGTC 
ATCTATGGAA AGGGAGTTGT TTGTACCCTG CATGAGGGAG ATGACTTTGG GAAGCTTGCC 
CTTGTTAACG ACGCCCCAAG GGCAGCATCC ATTGTTTTAC GAGAAGACAA TTGTCACTTC 
CTGCGTGTGG ACAAGGAGGA CTTCAACCGG ATCCTGCGTG ACGTGGAGGC CAACACAGTG 
AGACTCAAGG AGCACGATCA AGATGTCTTG GTACTAGAAA AAATCCTGGC TGGATCTCAA 
GGCTCAGCAC AAGGGAACAC TCAGTCGCCA TATAAATACA CTGTGATGTC AGGAAACCCA 
GAGAAACTTC TAGAACACTT CCTGGAAACA ATGCGCCTTG ATCCGAGTTT AAATGATGCA 
ACAGACACCG CTTTGGACGA CTTTATTCTG ATGCACTGTG TTTTTATGCC AAATTCTCAG 
CTCTGCCCGG CCTTGATGTC ACATTACCAT GCGCAGCCTT CCCAGGGAAC GGAGCAGGAG 
AAGCTGGATT ACACCCTGAA TAACAAGCGC AGGGTCATAC GGCTGGTTCA GCAGTGGGCC 
ACTCTGTATG GGGACCTGCT CCGTGAGGAT GAGGTTTCAA TGGCCTTTCT TGAAGAATTT 
TATGTGTCGG TATCAGATGA CACAAGAACG ACTGCAGCCC TGAAGGAGCA GCTACCTGAC 
CTAGAACGAG CCATCAAGCA AATATCTGAG GATGCAAAGG GGCAACAAAA GAAGCACAAG 
GTGCTTTTAC GGCAGTTCAG CACTGGAGAT GAGAGACTGC AAAAGCGCCA GGCAATACGG 
AGTTCCGATG AACTTCTGTT CAAGGTATAC TGCATAGACC ATACGTACAC CACTATCCGT 
GTCGCTGTGG CTGCTTCAGT GAAAGAAGTG CTCTCTGCGG TCGCCGATAA ACTAGGCTCC 
GGGGACAGCT TGATACTCGT CAAAATCAGT TCTGCAGGAG AAAAAGTGGT GCTGAAACCA 
AATGAAATAT CCGTCTTTAC AACGCTCAGC GTGAATGGAC GTCTGTTTGC CTGCCCCCGG 
GACCAGTTCG ATTCTTTGAC CCCTCTACCA GAACAAGAGG GGCCTTCCGC TGGAACCATG 
TCAACTTTTG AACTCATGAG CTCTAAAGAC TTGGCGTATC AGATGACCAT TCATGACTGG 
GACCTATTCA GTTGTGTGCA TGAGCTGGAA CTTATTTACC ACACATTCGG GCGACAGAAT 
TTCAAAAAGA CGACGGCAAA TTTGGACCTC TTCCTGAGAC GTTTTAATGA AATTCAGTTC 
TGGGTTGTGA CTGAGATTTG CCTGTGCCCT CAACTCAGCA AGCGCGTTCA ACTTCTAAAG 
AAATTTATTA AAATTGCGGC CCACTGCAAG GAATATAAAA ATTTGAACTC GTTCTTTGCT 
ATTGTCATGG GGCTGAGTAA CGTGGCCGTA AGCCGTCTCT CACTGACATG GGAGAAACTC 
CCAAGCAAGT TCAAAAAGAT CTATGCAGAA TTTGAAAATT TAATGGATCC ATCAAGGAAT 
CACAGGGCTT ACAGGCTCAC TGTAGCTAAA CTGGACCCTC CAATCATTCC ATTTACACCT 
CTGCTTATCA AAGATATGAC TTTTACCCAT GAGGGCAACA AAACCTTCAT TGACAATTTG 
GTTAACTTTG AGAAAATGCG AATGATCGCA AACACCTTGA GAACAATAAG ATACTGCCGA 
AGCGTCCCTT TCAATCCTGA TGCCTCTCTT GCCGGCAAAA ATCACCAAGA CGTGCGGAAT 
TATGTGCGCC AGTTCAACGT GATTGACAAC CAGAGGACAC TGTCTCAGAT GTCCCACCGG 
CTGGAACCAC GCCGAGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rapgef4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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