plekhg3 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012969108.3	XP_012824562.2	pleckstrin homology domain-containing family G member 3 isoform X3
XM_004917285.4	XP_004917342.3	pleckstrin homology domain-containing family G member 3 isoform X5
XM_004917286.4	XP_004917343.3	pleckstrin homology domain-containing family G member 3 isoform X6
XM_012969107.2	XP_012824561.1	pleckstrin homology domain-containing family G member 3 isoform X2
XM_018096659.1	XP_017952148.1	pleckstrin homology domain-containing family G member 3 isoform X3
XM_012969111.2	XP_012824565.1	pleckstrin homology domain-containing family G member 3 isoform X4
XM_004917286.3	XP_004917343.2	pleckstrin homology domain-containing family G member 3 isoform X6
XM_018096661.1	XP_017952150.1	pleckstrin homology domain-containing family G member 3 isoform X8
XM_012969112.3	XP_012824566.2	pleckstrin homology domain-containing family G member 3 isoform X7
XM_004917285.3	XP_004917342.2	pleckstrin homology domain-containing family G member 3 isoform X5
XM_004917284.4	XP_004917341.3	pleckstrin homology domain-containing family G member 3 isoform X1
XM_012969111.3	XP_012824565.2	pleckstrin homology domain-containing family G member 3 isoform X4
XM_004917284.3	XP_004917341.2	pleckstrin homology domain-containing family G member 3 isoform X1
XM_012969108.2	XP_012824562.1	pleckstrin homology domain-containing family G member 3 isoform X3
XM_012969112.2	XP_012824566.1	pleckstrin homology domain-containing family G member 3 isoform X7
XM_018096660.1	XP_017952149.1	pleckstrin homology domain-containing family G member 3 isoform X7
XM_012969107.3	XP_012824561.2	pleckstrin homology domain-containing family G member 3 isoform X2
XM_018096659.2	XP_017952148.2	pleckstrin homology domain-containing family G member 3 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa46321	XM_018096661.1 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa65741	XM_004917284.4 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65742	XM_012969107.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65743	XM_012969108.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65743	XM_018096659.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65744	XM_012969111.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65745	XM_004917285.4 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65746	XM_004917286.4 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa65747	XM_012969112.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36859	XM_004917284.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36860	XM_012969107.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36861	XM_018096659.1 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36861	XM_012969108.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36862	XM_012969111.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36863	XM_004917285.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36864	XM_004917286.3 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36865	XM_012969112.2 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36865	XM_018096660.1 Latest version!	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

plekhg3 ( XM_018096661.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952150.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa46321
Clone ID Related Accession (Same CDS sequence)	XM_018096661.1
Accession Version	XM_018096661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAATA AAACCCTGGC CAAGTTCTTT CGAGAGAAGC AGGAGATGCT GAACCAATCC 
CTTCCACTCG GCTCCTACCT CCTAAAGCCA GTGCAGAGAA TACTGAAGTA TCACCTCCTA 
TTGCAGGAAA TAGCAAAACA TCTTGATGTG AAGGTGGAAG GGTATGAGGT GCTGGAGGAG 
GCTATTGAAA CCATGACGGG AGTAGCCTGG TACATTAACG ACATGAAAAG AAAGCACGAA 
CATGCTGTGC GGCAGCAGGA GATCCAGTCT TTGCTGTTGA ACTGGAAAGG TATAGATCTC 
ACTGCATATG GAGAACTTGT GCTGGAGGGA ACATTTCGGC TCCAGCGAGC ACGGAGTGAG 
AGGACATTTT TCCTTTTCGA TAAGGCCCTG TTTATCACCA AGAGACGTGG GGATCACTAT 
GTCTACAAAA CTCATATCCC GTGTTCAAGC TTGATGCTGA TTGAGAGTGC CCGTGACTCC 
CTCTGCTTTA GTGTGACCCA TTACAAGAAC AGCAAACAGC AGCATAATGT GCAGGCCAAA 
ACAGTTGAAG AGAAACATGT GTGGACCCAT CATATAAAGA AACTCATACT AGAGAACCAC 
CATGCAATCA TTCCCCAAAA GGCGAAGGAG GCAATACTGG AAATGGATAA CATGTATAAA 
GGAAGGTACA AATACAGCCC AGAGAGGCAA AAGAAGACCA TGTCTGCCAT TGAGCCCTCC 
ATGACACACC GGTCAGGACG CAGACAGTCT GAGCCCTCCA AAGACATACT AAAGCAACTG 
AATGACAAAG CAGGATTAAA GCATGCAGGC AGTGATGGAG ATCTTCAGGA ATTTGGGGAC 
ATTCCACAGC CTTGTATTGG CAGCTTGGGC ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA 
GACAAGGAGC GTCCTGTCAG CACACAGCTG AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC 
TCTGATGAGG ATGAGGAGGA GATACAGTTG GGAGATGATG ATAATGATGA TGATGATGAG 
CAGACTAAAG ATCCGGTGGA GGAAGAGGCA CCAAAGGGCT GCAAGAGACA AAACAGCCAG 
TCATCTGGGA ATGCAGAGAA GAGGAGGAGT GTGGAGGTCA CATCTGGGCA CCAGGAGTCT 
GTATATAAAA TGGATAAGGA GAATTCAGAG CCAGAAGAGA TCTACAAGGC TGTGGTCGTC 
AGCTCTTCTG AGGATGAACA AGCTGTTCTG TTGGAAATCA CAGATGACAC AGAGCCAGCA 
AAAGATGATA GTGCAGCTCA CCCAGAAGTC CTTGAGCCTG TGGAGAATGA AGAAGCTGAA 
AGTACAGAAG ACTCAAAGAC TTTCAGCAGC GAAGAAGAGG AAGAGGAGAC ACCAACCCAT 
GAATCAACAA GTATCCTACC TCCTTCTGTT CTGGACCAGG CCAGTGTTAT TGCTGAGAGG 
TTTATAAGCA GCATGTCCAG GAGGAGTAGC ATGGCTCTAG ATGATGGGAA AGTCATAGGC 
TACATCACCC CCAGATTAAC CAGCCGCTGC AGTAGTTTGA TCAATCTGGA ATCTACAGAT 
ACTATTATTT ATCAGAACGG TTCAAGCGAC TTCCAGGACT TCTCAGAAGT TTGTGGTGAC 
ACTGGAGTAG ACATTCTTGA CACCACTTTT AGTAAGGTGA ACTCTGCCAG TATGGAGAAT 
GTGTTTGAGG AAAGAGAAAG GCCCACATGT AAGAGAAGAG ACTCTATTCT ATCCATGCAA 
GATAGACAAC TCATTGATAA AATCAGAACT TACTATGACG ATGCAGAAAA TCGGGATGCA 
TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG AACTATATTC CCACTGGACT TGTTAGAAAC 
TCTGTATTTA AGATTAACAG CCTGCCCAGA TTTGATACTA CCCAGAGTTC TCTATCGAGG 
CAGAGAATGT CCAGTTCATC TAGTAATACC AGCGGTGTGA ACAGTAGACA TGCTTCCTTG 
ACAGATACAA GGTCTAATGC AAGAGAATCA GAGCAGTTAG AGACGCAAAC TGAACTGCTT 
TTGGAAACAG CCACAAATGG CACTTCTGAC CATTCAGCCC CGATTACTGA TGAGGAGTTC 
CGGACACCAG ATGAAATGAT TAAAGTCTGG GATGAGTTTG AAAAGGTAAA TGCTTGGAAA 
GAGGCCTCAG ATATTTTAGA AGCCAAAAAA GATGTCAAAC CAGACTCTGT GGGTGAATGT 
ACAGCACACT GTGCCCTGGA GAATGGTTTG GAATCTCACG AACCACTGCT CATATTGGAA 
GACAGCGATC TGAGTACTAT AACAGAGGAA TCGCCTCTTC CTACACCGGA AAGCACATCT 
CCTAAACAGT TTGGCTGTGT TCCCAATAAC ACAAGTTTTA GTGAGTACAA AACGCCTGTG 
AAGCTACACT CCAAGATAAT GCAAATGGCC AGCTGCATGG ATGAAGACAT GTCGGAGAAG 
ATGAAGAACA AGGTGTACCA GCTGGCCAGG CAGTACAGTC AGAGGATCAA ATGCAACAAG 
CCAGTGTCCC AAAGGCAGCT AAAGGAGATA GAGGAGGATC TCAGAAGGAA CAGCCTGCCT 
GCTGTGCAAG AGGAAAAACT GGATAAGAAA GAGAAACGCA AGCCAATACT GTCACTCCCC 
ACATACGACA GCGTTATACT CCAGGAGCAT GGCCCTACAA CCCCAACATC GGCCCTGAGC 
AGAGACACAG CTCCAAAGCA TCTTTCCTTT TCACCATCCT GTGACAGCCC ACGGGCTCTT 
TCCCCAGAGA GAGCTGACTG CAGGAGCCCT CTGAGTCCAG TCAAGGCAGA GAGATTTCAC 
TGGCCTGACG TCCGAGAACT GAGATCTAGG TACACAAGTG CAGCCAGCTC TAGCAAGGCT 
CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA GAAAGAATAA CTGTGCCCAA TTTGAGGGGG 
CATTCTGACA CACAAAGTAA AGGTTGCTTG ATTCACTCTC ACAGCATGAA CGTGAAACAC 
ACAGAAAATG TCAATGGCAG CGCTGAGGAC TCGGCACAGA ATAACAAAGC ACAGGTAGAA 
ACTGAGCCGC CTTTGCCAGT GTCACCCAGT CTAAAAGGTA AAACTGATTC ACTACTTGGC 
TCTGCAAAGC CTGGCTATTA CATCAGTGCA GAGGCTCCTT TGGAAAATAA CAAGAAAGTT 
ATCGTTATGG AAAAAATTCC TGCTGCCACA GACTCTAATG GCTCCCATGA TGTGGAAAGC 
GATGACACTT ACGTACAAAT CCGATCCCCC ACAAGCAGAG AGAAGATCTC TCTCAAAGCC 
GTGATTGAGC GCTGCAAAGC CTACCAGGAG TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC 
TCCAAGGATG GGGAAACCAC TGAATGCACT GAATCTGTTC CCCATGAAGA GCCCAATCTG 
GAGAGGCACG TGGCACCAAG CTGCGATGTC AACCAGCAGA ACCGAGTCAA GAACTTGAGA 
GAAAAGTTTC AGACTAAGCA CAACTCAAGT AGTTTGAAAC AATCGGAGGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952150.1
CDS	227..3763
Translation	MRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_018096661.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096661.1

ORF Insert Sequence:

ATGAGGAATA AAACCCTGGC CAAGTTCTTT CGAGAGAAGC AGGAGATGCT GAACCAATCC 
CTTCCACTCG GCTCCTACCT CCTAAAGCCA GTGCAGAGAA TACTGAAGTA TCACCTCCTA 
TTGCAGGAAA TAGCAAAACA TCTTGATGTG AAGGTGGAAG GGTATGAGGT GCTGGAGGAG 
GCTATTGAAA CCATGACGGG AGTAGCCTGG TACATTAACG ACATGAAAAG AAAGCACGAA 
CATGCTGTGC GGCAGCAGGA GATCCAGTCT TTGCTGTTGA ACTGGAAAGG TATAGATCTC 
ACTGCATATG GAGAACTTGT GCTGGAGGGA ACATTTCGGC TCCAGCGAGC ACGGAGTGAG 
AGGACATTTT TCCTTTTCGA TAAGGCCCTG TTTATCACCA AGAGACGTGG GGATCACTAT 
GTCTACAAAA CTCATATCCC GTGTTCAAGC TTGATGCTGA TTGAGAGTGC CCGTGACTCC 
CTCTGCTTTA GTGTGACCCA TTACAAGAAC AGCAAACAGC AGCATAATGT GCAGGCCAAA 
ACAGTTGAAG AGAAACATGT GTGGACCCAT CATATAAAGA AACTCATACT AGAGAACCAC 
CATGCAATCA TTCCCCAAAA GGCGAAGGAG GCAATACTGG AAATGGATAA CATGTATAAA 
GGAAGGTACA AATACAGCCC AGAGAGGCAA AAGAAGACCA TGTCTGCCAT TGAGCCCTCC 
ATGACACACC GGTCAGGACG CAGACAGTCT GAGCCCTCCA AAGACATACT AAAGCAACTG 
AATGACAAAG CAGGATTAAA GCATGCAGGC AGTGATGGAG ATCTTCAGGA ATTTGGGGAC 
ATTCCACAGC CTTGTATTGG CAGCTTGGGC ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA 
GACAAGGAGC GTCCTGTCAG CACACAGCTG AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC 
TCTGATGAGG ATGAGGAGGA GATACAGTTG GGAGATGATG ATAATGATGA TGATGATGAG 
CAGACTAAAG ATCCGGTGGA GGAAGAGGCA CCAAAGGGCT GCAAGAGACA AAACAGCCAG 
TCATCTGGGA ATGCAGAGAA GAGGAGGAGT GTGGAGGTCA CATCTGGGCA CCAGGAGTCT 
GTATATAAAA TGGATAAGGA GAATTCAGAG CCAGAAGAGA TCTACAAGGC TGTGGTCGTC 
AGCTCTTCTG AGGATGAACA AGCTGTTCTG TTGGAAATCA CAGATGACAC AGAGCCAGCA 
AAAGATGATA GTGCAGCTCA CCCAGAAGTC CTTGAGCCTG TGGAGAATGA AGAAGCTGAA 
AGTACAGAAG ACTCAAAGAC TTTCAGCAGC GAAGAAGAGG AAGAGGAGAC ACCAACCCAT 
GAATCAACAA GTATCCTACC TCCTTCTGTT CTGGACCAGG CCAGTGTTAT TGCTGAGAGG 
TTTATAAGCA GCATGTCCAG GAGGAGTAGC ATGGCTCTAG ATGATGGGAA AGTCATAGGC 
TACATCACCC CCAGATTAAC CAGCCGCTGC AGTAGTTTGA TCAATCTGGA ATCTACAGAT 
ACTATTATTT ATCAGAACGG TTCAAGCGAC TTCCAGGACT TCTCAGAAGT TTGTGGTGAC 
ACTGGAGTAG ACATTCTTGA CACCACTTTT AGTAAGGTGA ACTCTGCCAG TATGGAGAAT 
GTGTTTGAGG AAAGAGAAAG GCCCACATGT AAGAGAAGAG ACTCTATTCT ATCCATGCAA 
GATAGACAAC TCATTGATAA AATCAGAACT TACTATGACG ATGCAGAAAA TCGGGATGCA 
TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG AACTATATTC CCACTGGACT TGTTAGAAAC 
TCTGTATTTA AGATTAACAG CCTGCCCAGA TTTGATACTA CCCAGAGTTC TCTATCGAGG 
CAGAGAATGT CCAGTTCATC TAGTAATACC AGCGGTGTGA ACAGTAGACA TGCTTCCTTG 
ACAGATACAA GGTCTAATGC AAGAGAATCA GAGCAGTTAG AGACGCAAAC TGAACTGCTT 
TTGGAAACAG CCACAAATGG CACTTCTGAC CATTCAGCCC CGATTACTGA TGAGGAGTTC 
CGGACACCAG ATGAAATGAT TAAAGTCTGG GATGAGTTTG AAAAGGTAAA TGCTTGGAAA 
GAGGCCTCAG ATATTTTAGA AGCCAAAAAA GATGTCAAAC CAGACTCTGT GGGTGAATGT 
ACAGCACACT GTGCCCTGGA GAATGGTTTG GAATCTCACG AACCACTGCT CATATTGGAA 
GACAGCGATC TGAGTACTAT AACAGAGGAA TCGCCTCTTC CTACACCGGA AAGCACATCT 
CCTAAACAGT TTGGCTGTGT TCCCAATAAC ACAAGTTTTA GTGAGTACAA AACGCCTGTG 
AAGCTACACT CCAAGATAAT GCAAATGGCC AGCTGCATGG ATGAAGACAT GTCGGAGAAG 
ATGAAGAACA AGGTGTACCA GCTGGCCAGG CAGTACAGTC AGAGGATCAA ATGCAACAAG 
CCAGTGTCCC AAAGGCAGCT AAAGGAGATA GAGGAGGATC TCAGAAGGAA CAGCCTGCCT 
GCTGTGCAAG AGGAAAAACT GGATAAGAAA GAGAAACGCA AGCCAATACT GTCACTCCCC 
ACATACGACA GCGTTATACT CCAGGAGCAT GGCCCTACAA CCCCAACATC GGCCCTGAGC 
AGAGACACAG CTCCAAAGCA TCTTTCCTTT TCACCATCCT GTGACAGCCC ACGGGCTCTT 
TCCCCAGAGA GAGCTGACTG CAGGAGCCCT CTGAGTCCAG TCAAGGCAGA GAGATTTCAC 
TGGCCTGACG TCCGAGAACT GAGATCTAGG TACACAAGTG CAGCCAGCTC TAGCAAGGCT 
CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA GAAAGAATAA CTGTGCCCAA TTTGAGGGGG 
CATTCTGACA CACAAAGTAA AGGTTGCTTG ATTCACTCTC ACAGCATGAA CGTGAAACAC 
ACAGAAAATG TCAATGGCAG CGCTGAGGAC TCGGCACAGA ATAACAAAGC ACAGGTAGAA 
ACTGAGCCGC CTTTGCCAGT GTCACCCAGT CTAAAAGGTA AAACTGATTC ACTACTTGGC 
TCTGCAAAGC CTGGCTATTA CATCAGTGCA GAGGCTCCTT TGGAAAATAA CAAGAAAGTT 
ATCGTTATGG AAAAAATTCC TGCTGCCACA GACTCTAATG GCTCCCATGA TGTGGAAAGC 
GATGACACTT ACGTACAAAT CCGATCCCCC ACAAGCAGAG AGAAGATCTC TCTCAAAGCC 
GTGATTGAGC GCTGCAAAGC CTACCAGGAG TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC 
TCCAAGGATG GGGAAACCAC TGAATGCACT GAATCTGTTC CCCATGAAGA GCCCAATCTG 
GAGAGGCACG TGGCACCAAG CTGCGATGTC AACCAGCAGA ACCGAGTCAA GAACTTGAGA 
GAAAAGTTTC AGACTAAGCA CAACTCAAGT AGTTTGAAAC AATCGGAGGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917284.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917341.3 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa65741
Clone ID Related Accession (Same CDS sequence)	XM_004917284.4
Accession Version	XM_004917284.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917284.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGCAGGAT TAAAGCATGC AGGCAGTGAT 
GGAGATCTTC AGGAATTTGG GGACATTCCA CAGCCTTGTA TTGGCAGCTT GGGCACCAGC 
CTTGAGCAGC CAGAGGCTGA GGGAGACAAG GAGCGTCCTG TCAGCACACA GCTGAGTAGA 
AGCTCAGTGG CAGAGCTCAG TGCCTCTGAT GAGGATGAGG AGGAGATACA GTTGGGAGAT 
GATGATAATG ATGATGATGA TGAGCAGACT AAAGATCCGG TGGAGGAAGA GGCACCAAAG 
GGCTGCAAGA GACAAAACAG CCAGTCATCT GGGAATGCAG AGAAGAGGAG GAGTGTGGAG 
GTCACATCTG GGCACCAGGA GTCTGTATAT AAAATGGATA AGGAGAATTC AGAGCCAGAA 
GAGATCTACA AGGCTGTGGT CGTCAGCTCT TCTGAGGATG AACAAGCTGT TCTGTTGGAA 
ATCACAGATG ACACAGAGCC AGCAAAAGAT GATAGTGCAG CTCACCCAGA AGTCCTTGAG 
CCTGTGGAGA ATGAAGAAGC TGAAAGTACA GAAGACTCAA AGACTTTCAG CAGCGAAGAA 
GAGGAAGAGG AGACACCAAC CCATGAATCA ACAAGTATCC TACCTCCTTC TGTTCTGGAC 
CAGGCCAGTG TTATTGCTGA GAGGTTTATA AGCAGCATGT CCAGGAGGAG TAGCATGGCT 
CTAGATGATG GGAAAGTCAT AGGCTACATC ACCCCCAGAT TAACCAGCCG CTGCAGTAGT 
TTGATCAATC TGGAATCTAC AGATACTATT ATTTATCAGA ACGGTTCAAG CGACTTCCAG 
GACTTCTCAG AAGTTTGTGG TGACACTGGA GTAGACATTC TTGACACCAC TTTTAGTAAG 
GTGAACTCTG CCAGTATGGA GAATGTGTTT GAGGAAAGAG AAAGGCCCAC ATGTAAGAGA 
AGAGACTCTA TTCTATCCAT GCAAGATAGA CAACTCATTG ATAAAATCAG AACTTACTAT 
GACGATGCAG AAAATCGGGA TGCATCCTTT AGCATTAAGC GCAGAGAGAG CTTGAACTAT 
ATTCCCACTG GACTTGTTAG AAACTCTGTA TTTAAGATTA ACAGCCTGCC CAGATTTGAT 
ACTACCCAGA GTTCTCTATC GAGGCAGAGA ATGTCCAGTT CATCTAGTAA TACCAGCGGT 
GTGAACAGTA GACATGCTTC CTTGACAGAT ACAAGGTCTA ATGCAAGAGA ATCAGAGCAG 
TTAGAGACGC AAACTGAACT GCTTTTGGAA ACAGCCACAA ATGGCACTTC TGACCATTCA 
GCCCCGATTA CTGATGAGGA GTTCCGGACA CCAGATGAAA TGATTAAAGT CTGGGATGAG 
TTTGAAAAGG TAAATGCTTG GAAAGAGGCC TCAGATATTT TAGAAGCCAA AAAAGATGTC 
AAACCAGACT CTGTGGGTGA AAGTACAGCA CACTGTGCCC TGGAGAATGG CTTGGAATCT 
CACGAACCAC TGCTCATATT GGAAGACAGC GATCTGAGTA CTATAACAGA GGAATCGCCT 
CTTCCTACAC CGGAAAGCAC ATCTCCTAAA CAGTTTGGCT GTGTTCCCAA TAACACAAGT 
TTTAGTGAGT ACAAAACGCC TGTGAAGCTA CACTCCAAGA TAATGCAAAT GGCCAGCTGC 
ATGGATGAAG ACATGTCGGA GAAGATGAAG AACAAGGTGT ACCAGCTGGC CAGGCAGTAC 
AGTCAGAGGA TCAAATGCAA CAAGCCAGTG TCCCAAAGGC AGCTAAAGGA GATAGAGGAG 
GATTTCAGAA GGAACAGCCT GCCTGCTGTG CAAGAGGAAA AACTGGATAA GAAAGAGAAA 
CGCAAGCCAA TACTGTCACT CCCCACATAC GACAGCGTTA TACTCCAGGA GCATGGCCCT 
ACAACCCCAA CATCGGCCCT GAGCAGAGAC ACAGCTCCAA AGCATCTTTC CTTTTCGCCA 
TCCTGTGACA GCCCACGGGC TCTTTCCCCA GAGAGAGCTG ACTGCAGGAG CCCTCTGAGT 
CCAGTCAAGG CAGAGAGATT TCACTGGCCT GACGTCCGAG AACTGAGATC TAGGTACACA 
AGTGCAGCCA GCTCTAGCAA GGCTCCTCCC GTCAACAGGA GCCAGTCTGT GCCAGAAAGA 
ATAACTGTGC CCAATTTGAG GGGGCATTCT GACACACAAA GTAAGGGTTG CTTGATTCAC 
TCTCACAGCA TGAACGTGAA ACACACAGAA AATGTCAATG GCAGCGCTGA GGACTCGGCA 
CAGAATAACA AAGCACAGGT AGAAACTGAG CCGCCTTTGC CAGTGTCACC CAGTCTAAAA 
GGTAAAACTG ATTCACTACT TGGCTCTGCA AAGCCTGGCT ATTACATCAG TGCAGAGGCT 
CCTTTGGAAA ATAACAAGAA AGTTATCGTT ATGGAAAAAA TTCCTGCTGC CACAGACTCT 
AATGGCTCCC ATGATGTGGA AAGCGATGAC ACTTACGTAC AAATCCGATC CCCCACAAGC 
AGAGAGAAGA TCTCTCTCAA AGCCGTGATT GAGCGCTGCA AAGCCTACCA GGAGTCCGAG 
GAGTACCGGT CCAGGGAAGT AGCCTCCAAG GATGGGGAAA CCACTGAATG CACTGAATCT 
GTTCCCCATG AAGAGCCCAA TCTGGAGAGG CACGTGGCAC CAAGCTGCGA TGTCAACCAG 
CAGAACCGAG TCAAGAACTT GAGAGAAAAG TTTCAGACTA AGCACAACTC AAGTAGTTTG 
AAACAATCGG AAGTTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917341.3
CDS	42..4442
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917284.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917284.4

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGCAGGAT TAAAGCATGC AGGCAGTGAT 
GGAGATCTTC AGGAATTTGG GGACATTCCA CAGCCTTGTA TTGGCAGCTT GGGCACCAGC 
CTTGAGCAGC CAGAGGCTGA GGGAGACAAG GAGCGTCCTG TCAGCACACA GCTGAGTAGA 
AGCTCAGTGG CAGAGCTCAG TGCCTCTGAT GAGGATGAGG AGGAGATACA GTTGGGAGAT 
GATGATAATG ATGATGATGA TGAGCAGACT AAAGATCCGG TGGAGGAAGA GGCACCAAAG 
GGCTGCAAGA GACAAAACAG CCAGTCATCT GGGAATGCAG AGAAGAGGAG GAGTGTGGAG 
GTCACATCTG GGCACCAGGA GTCTGTATAT AAAATGGATA AGGAGAATTC AGAGCCAGAA 
GAGATCTACA AGGCTGTGGT CGTCAGCTCT TCTGAGGATG AACAAGCTGT TCTGTTGGAA 
ATCACAGATG ACACAGAGCC AGCAAAAGAT GATAGTGCAG CTCACCCAGA AGTCCTTGAG 
CCTGTGGAGA ATGAAGAAGC TGAAAGTACA GAAGACTCAA AGACTTTCAG CAGCGAAGAA 
GAGGAAGAGG AGACACCAAC CCATGAATCA ACAAGTATCC TACCTCCTTC TGTTCTGGAC 
CAGGCCAGTG TTATTGCTGA GAGGTTTATA AGCAGCATGT CCAGGAGGAG TAGCATGGCT 
CTAGATGATG GGAAAGTCAT AGGCTACATC ACCCCCAGAT TAACCAGCCG CTGCAGTAGT 
TTGATCAATC TGGAATCTAC AGATACTATT ATTTATCAGA ACGGTTCAAG CGACTTCCAG 
GACTTCTCAG AAGTTTGTGG TGACACTGGA GTAGACATTC TTGACACCAC TTTTAGTAAG 
GTGAACTCTG CCAGTATGGA GAATGTGTTT GAGGAAAGAG AAAGGCCCAC ATGTAAGAGA 
AGAGACTCTA TTCTATCCAT GCAAGATAGA CAACTCATTG ATAAAATCAG AACTTACTAT 
GACGATGCAG AAAATCGGGA TGCATCCTTT AGCATTAAGC GCAGAGAGAG CTTGAACTAT 
ATTCCCACTG GACTTGTTAG AAACTCTGTA TTTAAGATTA ACAGCCTGCC CAGATTTGAT 
ACTACCCAGA GTTCTCTATC GAGGCAGAGA ATGTCCAGTT CATCTAGTAA TACCAGCGGT 
GTGAACAGTA GACATGCTTC CTTGACAGAT ACAAGGTCTA ATGCAAGAGA ATCAGAGCAG 
TTAGAGACGC AAACTGAACT GCTTTTGGAA ACAGCCACAA ATGGCACTTC TGACCATTCA 
GCCCCGATTA CTGATGAGGA GTTCCGGACA CCAGATGAAA TGATTAAAGT CTGGGATGAG 
TTTGAAAAGG TAAATGCTTG GAAAGAGGCC TCAGATATTT TAGAAGCCAA AAAAGATGTC 
AAACCAGACT CTGTGGGTGA AAGTACAGCA CACTGTGCCC TGGAGAATGG CTTGGAATCT 
CACGAACCAC TGCTCATATT GGAAGACAGC GATCTGAGTA CTATAACAGA GGAATCGCCT 
CTTCCTACAC CGGAAAGCAC ATCTCCTAAA CAGTTTGGCT GTGTTCCCAA TAACACAAGT 
TTTAGTGAGT ACAAAACGCC TGTGAAGCTA CACTCCAAGA TAATGCAAAT GGCCAGCTGC 
ATGGATGAAG ACATGTCGGA GAAGATGAAG AACAAGGTGT ACCAGCTGGC CAGGCAGTAC 
AGTCAGAGGA TCAAATGCAA CAAGCCAGTG TCCCAAAGGC AGCTAAAGGA GATAGAGGAG 
GATTTCAGAA GGAACAGCCT GCCTGCTGTG CAAGAGGAAA AACTGGATAA GAAAGAGAAA 
CGCAAGCCAA TACTGTCACT CCCCACATAC GACAGCGTTA TACTCCAGGA GCATGGCCCT 
ACAACCCCAA CATCGGCCCT GAGCAGAGAC ACAGCTCCAA AGCATCTTTC CTTTTCGCCA 
TCCTGTGACA GCCCACGGGC TCTTTCCCCA GAGAGAGCTG ACTGCAGGAG CCCTCTGAGT 
CCAGTCAAGG CAGAGAGATT TCACTGGCCT GACGTCCGAG AACTGAGATC TAGGTACACA 
AGTGCAGCCA GCTCTAGCAA GGCTCCTCCC GTCAACAGGA GCCAGTCTGT GCCAGAAAGA 
ATAACTGTGC CCAATTTGAG GGGGCATTCT GACACACAAA GTAAGGGTTG CTTGATTCAC 
TCTCACAGCA TGAACGTGAA ACACACAGAA AATGTCAATG GCAGCGCTGA GGACTCGGCA 
CAGAATAACA AAGCACAGGT AGAAACTGAG CCGCCTTTGC CAGTGTCACC CAGTCTAAAA 
GGTAAAACTG ATTCACTACT TGGCTCTGCA AAGCCTGGCT ATTACATCAG TGCAGAGGCT 
CCTTTGGAAA ATAACAAGAA AGTTATCGTT ATGGAAAAAA TTCCTGCTGC CACAGACTCT 
AATGGCTCCC ATGATGTGGA AAGCGATGAC ACTTACGTAC AAATCCGATC CCCCACAAGC 
AGAGAGAAGA TCTCTCTCAA AGCCGTGATT GAGCGCTGCA AAGCCTACCA GGAGTCCGAG 
GAGTACCGGT CCAGGGAAGT AGCCTCCAAG GATGGGGAAA CCACTGAATG CACTGAATCT 
GTTCCCCATG AAGAGCCCAA TCTGGAGAGG CACGTGGCAC CAAGCTGCGA TGTCAACCAG 
CAGAACCGAG TCAAGAACTT GAGAGAAAAG TTTCAGACTA AGCACAACTC AAGTAGTTTG 
AAACAATCGG AAGTTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969107.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824561.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.

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CloneID	OXa65742
Clone ID Related Accession (Same CDS sequence)	XM_012969107.3
Accession Version	XM_012969107.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969107.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGGATTAA AGCATGCAGG CAGTGATGGA 
GATCTTCAGG AATTTGGGGA CATTCCACAG CCTTGTATTG GCAGCTTGGG CACCAGCCTT 
GAGCAGCCAG AGGCTGAGGG AGACAAGGAG CGTCCTGTCA GCACACAGCT GAGTAGAAGC 
TCAGTGGCAG AGCTCAGTGC CTCTGATGAG GATGAGGAGG AGATACAGTT GGGAGATGAT 
GATAATGATG ATGATGATGA GCAGACTAAA GATCCGGTGG AGGAAGAGGC ACCAAAGGGC 
TGCAAGAGAC AAAACAGCCA GTCATCTGGG AATGCAGAGA AGAGGAGGAG TGTGGAGGTC 
ACATCTGGGC ACCAGGAGTC TGTATATAAA ATGGATAAGG AGAATTCAGA GCCAGAAGAG 
ATCTACAAGG CTGTGGTCGT CAGCTCTTCT GAGGATGAAC AAGCTGTTCT GTTGGAAATC 
ACAGATGACA CAGAGCCAGC AAAAGATGAT AGTGCAGCTC ACCCAGAAGT CCTTGAGCCT 
GTGGAGAATG AAGAAGCTGA AAGTACAGAA GACTCAAAGA CTTTCAGCAG CGAAGAAGAG 
GAAGAGGAGA CACCAACCCA TGAATCAACA AGTATCCTAC CTCCTTCTGT TCTGGACCAG 
GCCAGTGTTA TTGCTGAGAG GTTTATAAGC AGCATGTCCA GGAGGAGTAG CATGGCTCTA 
GATGATGGGA AAGTCATAGG CTACATCACC CCCAGATTAA CCAGCCGCTG CAGTAGTTTG 
ATCAATCTGG AATCTACAGA TACTATTATT TATCAGAACG GTTCAAGCGA CTTCCAGGAC 
TTCTCAGAAG TTTGTGGTGA CACTGGAGTA GACATTCTTG ACACCACTTT TAGTAAGGTG 
AACTCTGCCA GTATGGAGAA TGTGTTTGAG GAAAGAGAAA GGCCCACATG TAAGAGAAGA 
GACTCTATTC TATCCATGCA AGATAGACAA CTCATTGATA AAATCAGAAC TTACTATGAC 
GATGCAGAAA ATCGGGATGC ATCCTTTAGC ATTAAGCGCA GAGAGAGCTT GAACTATATT 
CCCACTGGAC TTGTTAGAAA CTCTGTATTT AAGATTAACA GCCTGCCCAG ATTTGATACT 
ACCCAGAGTT CTCTATCGAG GCAGAGAATG TCCAGTTCAT CTAGTAATAC CAGCGGTGTG 
AACAGTAGAC ATGCTTCCTT GACAGATACA AGGTCTAATG CAAGAGAATC AGAGCAGTTA 
GAGACGCAAA CTGAACTGCT TTTGGAAACA GCCACAAATG GCACTTCTGA CCATTCAGCC 
CCGATTACTG ATGAGGAGTT CCGGACACCA GATGAAATGA TTAAAGTCTG GGATGAGTTT 
GAAAAGGTAA ATGCTTGGAA AGAGGCCTCA GATATTTTAG AAGCCAAAAA AGATGTCAAA 
CCAGACTCTG TGGGTGAAAG TACAGCACAC TGTGCCCTGG AGAATGGCTT GGAATCTCAC 
GAACCACTGC TCATATTGGA AGACAGCGAT CTGAGTACTA TAACAGAGGA ATCGCCTCTT 
CCTACACCGG AAAGCACATC TCCTAAACAG TTTGGCTGTG TTCCCAATAA CACAAGTTTT 
AGTGAGTACA AAACGCCTGT GAAGCTACAC TCCAAGATAA TGCAAATGGC CAGCTGCATG 
GATGAAGACA TGTCGGAGAA GATGAAGAAC AAGGTGTACC AGCTGGCCAG GCAGTACAGT 
CAGAGGATCA AATGCAACAA GCCAGTGTCC CAAAGGCAGC TAAAGGAGAT AGAGGAGGAT 
TTCAGAAGGA ACAGCCTGCC TGCTGTGCAA GAGGAAAAAC TGGATAAGAA AGAGAAACGC 
AAGCCAATAC TGTCACTCCC CACATACGAC AGCGTTATAC TCCAGGAGCA TGGCCCTACA 
ACCCCAACAT CGGCCCTGAG CAGAGACACA GCTCCAAAGC ATCTTTCCTT TTCGCCATCC 
TGTGACAGCC CACGGGCTCT TTCCCCAGAG AGAGCTGACT GCAGGAGCCC TCTGAGTCCA 
GTCAAGGCAG AGAGATTTCA CTGGCCTGAC GTCCGAGAAC TGAGATCTAG GTACACAAGT 
GCAGCCAGCT CTAGCAAGGC TCCTCCCGTC AACAGGAGCC AGTCTGTGCC AGAAAGAATA 
ACTGTGCCCA ATTTGAGGGG GCATTCTGAC ACACAAAGTA AGGGTTGCTT GATTCACTCT 
CACAGCATGA ACGTGAAACA CACAGAAAAT GTCAATGGCA GCGCTGAGGA CTCGGCACAG 
AATAACAAAG CACAGGTAGA AACTGAGCCG CCTTTGCCAG TGTCACCCAG TCTAAAAGGT 
AAAACTGATT CACTACTTGG CTCTGCAAAG CCTGGCTATT ACATCAGTGC AGAGGCTCCT 
TTGGAAAATA ACAAGAAAGT TATCGTTATG GAAAAAATTC CTGCTGCCAC AGACTCTAAT 
GGCTCCCATG ATGTGGAAAG CGATGACACT TACGTACAAA TCCGATCCCC CACAAGCAGA 
GAGAAGATCT CTCTCAAAGC CGTGATTGAG CGCTGCAAAG CCTACCAGGA GTCCGAGGAG 
TACCGGTCCA GGGAAGTAGC CTCCAAGGAT GGGGAAACCA CTGAATGCAC TGAATCTGTT 
CCCCATGAAG AGCCCAATCT GGAGAGGCAC GTGGCACCAA GCTGCGATGT CAACCAGCAG 
AACCGAGTCA AGAACTTGAG AGAAAAGTTT CAGACTAAGC ACAACTCAAG TAGTTTGAAA 
CAATCGGAAG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824561.2
CDS	42..4439
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969107.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969107.3

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGGATTAA AGCATGCAGG CAGTGATGGA 
GATCTTCAGG AATTTGGGGA CATTCCACAG CCTTGTATTG GCAGCTTGGG CACCAGCCTT 
GAGCAGCCAG AGGCTGAGGG AGACAAGGAG CGTCCTGTCA GCACACAGCT GAGTAGAAGC 
TCAGTGGCAG AGCTCAGTGC CTCTGATGAG GATGAGGAGG AGATACAGTT GGGAGATGAT 
GATAATGATG ATGATGATGA GCAGACTAAA GATCCGGTGG AGGAAGAGGC ACCAAAGGGC 
TGCAAGAGAC AAAACAGCCA GTCATCTGGG AATGCAGAGA AGAGGAGGAG TGTGGAGGTC 
ACATCTGGGC ACCAGGAGTC TGTATATAAA ATGGATAAGG AGAATTCAGA GCCAGAAGAG 
ATCTACAAGG CTGTGGTCGT CAGCTCTTCT GAGGATGAAC AAGCTGTTCT GTTGGAAATC 
ACAGATGACA CAGAGCCAGC AAAAGATGAT AGTGCAGCTC ACCCAGAAGT CCTTGAGCCT 
GTGGAGAATG AAGAAGCTGA AAGTACAGAA GACTCAAAGA CTTTCAGCAG CGAAGAAGAG 
GAAGAGGAGA CACCAACCCA TGAATCAACA AGTATCCTAC CTCCTTCTGT TCTGGACCAG 
GCCAGTGTTA TTGCTGAGAG GTTTATAAGC AGCATGTCCA GGAGGAGTAG CATGGCTCTA 
GATGATGGGA AAGTCATAGG CTACATCACC CCCAGATTAA CCAGCCGCTG CAGTAGTTTG 
ATCAATCTGG AATCTACAGA TACTATTATT TATCAGAACG GTTCAAGCGA CTTCCAGGAC 
TTCTCAGAAG TTTGTGGTGA CACTGGAGTA GACATTCTTG ACACCACTTT TAGTAAGGTG 
AACTCTGCCA GTATGGAGAA TGTGTTTGAG GAAAGAGAAA GGCCCACATG TAAGAGAAGA 
GACTCTATTC TATCCATGCA AGATAGACAA CTCATTGATA AAATCAGAAC TTACTATGAC 
GATGCAGAAA ATCGGGATGC ATCCTTTAGC ATTAAGCGCA GAGAGAGCTT GAACTATATT 
CCCACTGGAC TTGTTAGAAA CTCTGTATTT AAGATTAACA GCCTGCCCAG ATTTGATACT 
ACCCAGAGTT CTCTATCGAG GCAGAGAATG TCCAGTTCAT CTAGTAATAC CAGCGGTGTG 
AACAGTAGAC ATGCTTCCTT GACAGATACA AGGTCTAATG CAAGAGAATC AGAGCAGTTA 
GAGACGCAAA CTGAACTGCT TTTGGAAACA GCCACAAATG GCACTTCTGA CCATTCAGCC 
CCGATTACTG ATGAGGAGTT CCGGACACCA GATGAAATGA TTAAAGTCTG GGATGAGTTT 
GAAAAGGTAA ATGCTTGGAA AGAGGCCTCA GATATTTTAG AAGCCAAAAA AGATGTCAAA 
CCAGACTCTG TGGGTGAAAG TACAGCACAC TGTGCCCTGG AGAATGGCTT GGAATCTCAC 
GAACCACTGC TCATATTGGA AGACAGCGAT CTGAGTACTA TAACAGAGGA ATCGCCTCTT 
CCTACACCGG AAAGCACATC TCCTAAACAG TTTGGCTGTG TTCCCAATAA CACAAGTTTT 
AGTGAGTACA AAACGCCTGT GAAGCTACAC TCCAAGATAA TGCAAATGGC CAGCTGCATG 
GATGAAGACA TGTCGGAGAA GATGAAGAAC AAGGTGTACC AGCTGGCCAG GCAGTACAGT 
CAGAGGATCA AATGCAACAA GCCAGTGTCC CAAAGGCAGC TAAAGGAGAT AGAGGAGGAT 
TTCAGAAGGA ACAGCCTGCC TGCTGTGCAA GAGGAAAAAC TGGATAAGAA AGAGAAACGC 
AAGCCAATAC TGTCACTCCC CACATACGAC AGCGTTATAC TCCAGGAGCA TGGCCCTACA 
ACCCCAACAT CGGCCCTGAG CAGAGACACA GCTCCAAAGC ATCTTTCCTT TTCGCCATCC 
TGTGACAGCC CACGGGCTCT TTCCCCAGAG AGAGCTGACT GCAGGAGCCC TCTGAGTCCA 
GTCAAGGCAG AGAGATTTCA CTGGCCTGAC GTCCGAGAAC TGAGATCTAG GTACACAAGT 
GCAGCCAGCT CTAGCAAGGC TCCTCCCGTC AACAGGAGCC AGTCTGTGCC AGAAAGAATA 
ACTGTGCCCA ATTTGAGGGG GCATTCTGAC ACACAAAGTA AGGGTTGCTT GATTCACTCT 
CACAGCATGA ACGTGAAACA CACAGAAAAT GTCAATGGCA GCGCTGAGGA CTCGGCACAG 
AATAACAAAG CACAGGTAGA AACTGAGCCG CCTTTGCCAG TGTCACCCAG TCTAAAAGGT 
AAAACTGATT CACTACTTGG CTCTGCAAAG CCTGGCTATT ACATCAGTGC AGAGGCTCCT 
TTGGAAAATA ACAAGAAAGT TATCGTTATG GAAAAAATTC CTGCTGCCAC AGACTCTAAT 
GGCTCCCATG ATGTGGAAAG CGATGACACT TACGTACAAA TCCGATCCCC CACAAGCAGA 
GAGAAGATCT CTCTCAAAGC CGTGATTGAG CGCTGCAAAG CCTACCAGGA GTCCGAGGAG 
TACCGGTCCA GGGAAGTAGC CTCCAAGGAT GGGGAAACCA CTGAATGCAC TGAATCTGTT 
CCCCATGAAG AGCCCAATCT GGAGAGGCAC GTGGCACCAA GCTGCGATGT CAACCAGCAG 
AACCGAGTCA AGAACTTGAG AGAAAAGTTT CAGACTAAGC ACAACTCAAG TAGTTTGAAA 
CAATCGGAAG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969108.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824562.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.

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CloneID	OXa65743
Clone ID Related Accession (Same CDS sequence)	XM_012969108.3 , XM_018096659.2
Accession Version	XM_012969108.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCTAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAGC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824562.2
CDS	149..4567
Translation	MSTAPGLQTLWLSKVGSEGEEPQTLHSKETSTDGDLLTSESLRRHSKDDRQLLFLEETSHQGNDESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969108.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969108.3

ORF Insert Sequence:

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCTAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAGC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_018096659.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952148.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.

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CloneID	OXa65743
Clone ID Related Accession (Same CDS sequence)	XM_012969108.3 , XM_018096659.2
Accession Version	XM_018096659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCTAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAGC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952148.2
CDS	104..4522
Translation	MSTAPGLQTLWLSKVGSEGEEPQTLHSKETSTDGDLLTSESLRRHSKDDRQLLFLEETSHQGNDESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_018096659.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096659.2

ORF Insert Sequence:

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCTAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAGC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969111.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824565.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa65744
Clone ID Related Accession (Same CDS sequence)	XM_012969111.3
Accession Version	XM_012969111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGATG ATCGTGGGGG ACGCTTCGAA GGGCTTAATC GCAGGATCTC AAGGTTATTT 
GAATCCCCAC GGCTGTCTGC CGCATCCTCA AGCAGCAATG AAAAGCTCTC CATCTCAGCT 
TCAATCTGCA GTGATTCTGA TAGGCCCTTG AGTCTCTTCT CAACAGAGTC CACAGGCTCT 
TTACGTGAAG GCTGTGATTC CTATGAAGAT AACAGCGACG TACCGCTGGG AAACGGAACA 
GTGGTTCACA ACATGGAACT GGTGGCCACT GCAGCAGAGA GCCTGCAGGC CACCTACAGG 
GAGATGCAGA ACGGTCTGAG GAACAACCAC ACACCTAACA ATAATTCTGC CAATCGACGC 
AGCCACCAGG CGTCTAGGTC CCTTTCTCCA TTTGGTAACA AGGTCAATGC CATTACTCAC 
AAACTCAGCT ATGTGGAACG GGTGGTTCTG GAGATCAATG ACACAGAAAG AATGTATGTC 
CAGGATCTGC GCAGCATTGT GGAGGATTAC CTGGGAGCAA TCATAGACAG ACAGGAAGAG 
CTGCCTATAA CCCCAGAGCA AGTCTGTGCT CTCTTTGGAA ACATTGAGGA CATTTATGAA 
CTGAACAGTG AGCTGCTCCA GGACCTGGAT AATTGCAGGA ATGACCCCGT GGCCGTGGCC 
AGCTGCTTTG TCGAAAAGAG TCAGGACTTT GACATCTACA CCCAGTACTG CACCAACTAT 
CCCAACTCCG TGGCCACCTT GACGGAGTGC ATGAGGAATA AAACCCTGGC CAAGTTCTTT 
CGAGAGAAGC AGGAGATGCT GAACCAATCC CTTCCACTCG GCTCCTACCT CCTAAAGCCA 
GTGCAGAGAA TACTGAAGTA TCACCTCCTA TTGCAGGAAA TAGCAAAACA TCTTGATGTG 
AAGGTGGAAG GGTATGAGGT GCTGGAGGAG GCTATTGAAA CCATGACGGG AGTAGCCTGG 
TACATTAACG ACATGAAAAG AAAGCACGAA CATGCTGTGC GGCAGCAGGA GATCCAGTCT 
TTGCTGTTGA ACTGGAAAGG TATAGATCTC ACTGCATATG GAGAACTTGT GCTGGAGGGA 
ACATTTCGGC TCCAGCGAGC ACGGAGTGAG AGGACATTTT TCCTTTTCGA TAAGGCCCTG 
TTTATCACCA AGAGACGTGG GGATCACTAT GTCTACAAAA CTCATATCCC GTGTTCAAGC 
TTGATGCTGA TTGAGAGTGC CCGTGACTCC CTCTGCTTTA GTGTGACCCA TTACAAGAAC 
AGCAAACAGC AGCATAATGT GCAGGCCAAA ACAGTTGAAG AGAAACATGT GTGGACCCAT 
CATATAAAGA AACTCATACT AGAGAACCAC CATGCAATCA TTCCCCAAAA GGCGAAGGAG 
GCAATACTGG AAATGGATAA CATGTATAAA GGAAGGTACA AATACAGCCC AGAGAGGCAA 
AAGAAGACCA TGTCTGCCAT TGAGCCCTCC ATGACACACC GGTCAGGACG CAGACAGTCT 
GAGCCCTCCA AAGACATACT AAAGCAGCTG AATGACAAAG CAGGATTAAA GCATGCAGGC 
AGTGATGGAG ATCTTCAGGA ATTTGGGGAC ATTCCACAGC CTTGTATTGG CAGCTTGGGC 
ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA GACAAGGAGC GTCCTGTCAG CACACAGCTG 
AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC TCTGATGAGG ATGAGGAGGA GATACAGTTG 
GGAGATGATG ATAATGATGA TGATGATGAG CAGACTAAAG ATCCGGTGGA GGAAGAGGCA 
CCAAAGGGCT GCAAGAGACA AAACAGCCAG TCATCTGGGA ATGCAGAGAA GAGGAGGAGT 
GTGGAGGTCA CATCTGGGCA CCAGGAGTCT GTATATAAAA TGGATAAGGA GAATTCAGAG 
CCAGAAGAGA TCTACAAGGC TGTGGTCGTC AGCTCTTCTG AGGATGAACA AGCTGTTCTG 
TTGGAAATCA CAGATGACAC AGAGCCAGCA AAAGATGATA GTGCAGCTCA CCCAGAAGTC 
CTTGAGCCTG TGGAGAATGA AGAAGCTGAA AGTACAGAAG ACTCAAAGAC TTTCAGCAGC 
GAAGAAGAGG AAGAGGAGAC ACCAACCCAT GAATCAACAA GTATCCTACC TCCTTCTGTT 
CTGGACCAGG CCAGTGTTAT TGCTGAGAGG TTTATAAGCA GCATGTCCAG GAGGAGTAGC 
ATGGCTCTAG ATGATGGGAA AGTCATAGGC TACATCACCC CCAGATTAAC CAGCCGCTGC 
AGTAGTTTGA TCAATCTGGA ATCTACAGAT ACTATTATTT ATCAGAACGG TTCAAGCGAC 
TTCCAGGACT TCTCAGAAGT TTGTGGTGAC ACTGGAGTAG ACATTCTTGA CACCACTTTT 
AGTAAGGTGA ACTCTGCCAG TATGGAGAAT GTGTTTGAGG AAAGAGAAAG GCCCACATGT 
AAGAGAAGAG ACTCTATTCT ATCCATGCAA GATAGACAAC TCATTGATAA AATCAGAACT 
TACTATGACG ATGCAGAAAA TCGGGATGCA TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG 
AACTATATTC CCACTGGACT TGTTAGAAAC TCTGTATTTA AGATTAACAG CCTGCCCAGA 
TTTGATACTA CCCAGAGTTC TCTATCGAGG CAGAGAATGT CCAGTTCATC TAGTAATACC 
AGCGGTGTGA ACAGTAGACA TGCTTCCTTG ACAGATACAA GGTCTAATGC AAGAGAATCA 
GAGCAGTTAG AGACGCAAAC TGAACTGCTT TTGGAAACAG CCACAAATGG CACTTCTGAC 
CATTCAGCCC CGATTACTGA TGAGGAGTTC CGGACACCAG ATGAAATGAT TAAAGTCTGG 
GATGAGTTTG AAAAGGTAAA TGCTTGGAAA GAGGCCTCAG ATATTTTAGA AGCCAAAAAA 
GATGTCAAAC CAGACTCTGT GGGTGAAAGT ACAGCACACT GTGCCCTGGA GAATGGCTTG 
GAATCTCACG AACCACTGCT CATATTGGAA GACAGCGATC TGAGTACTAT AACAGAGGAA 
TCGCCTCTTC CTACACCGGA AAGCACATCT CCTAAACAGT TTGGCTGTGT TCCCAATAAC 
ACAAGTTTTA GTGAGTACAA AACGCCTGTG AAGCTACACT CCAAGATAAT GCAAATGGCC 
AGCTGCATGG ATGAAGACAT GTCGGAGAAG ATGAAGAACA AGGTGTACCA GCTGGCCAGG 
CAGTACAGTC AGAGGATCAA ATGCAACAAG CCAGTGTCCC AAAGGCAGCT AAAGGAGATA 
GAGGAGGATT TCAGAAGGAA CAGCCTGCCT GCTGTGCAAG AGGAAAAACT GGATAAGAAA 
GAGAAACGCA AGCCAATACT GTCACTCCCC ACATACGACA GCGTTATACT CCAGGAGCAT 
GGCCCTACAA CCCCAACATC GGCCCTGAGC AGAGACACAG CTCCAAAGCA TCTTTCCTTT 
TCGCCATCCT GTGACAGCCC ACGGGCTCTT TCCCCAGAGA GAGCTGACTG CAGGAGCCCT 
CTGAGTCCAG TCAAGGCAGA GAGATTTCAC TGGCCTGACG TCCGAGAACT GAGATCTAGG 
TACACAAGTG CAGCCAGCTC TAGCAAGGCT CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA 
GAAAGAATAA CTGTGCCCAA TTTGAGGGGG CATTCTGACA CACAAAGTAA GGGTTGCTTG 
ATTCACTCTC ACAGCATGAA CGTGAAACAC ACAGAAAATG TCAATGGCAG CGCTGAGGAC 
TCGGCACAGA ATAACAAAGC ACAGGTAGAA ACTGAGCCGC CTTTGCCAGT GTCACCCAGT 
CTAAAAGGTA AAACTGATTC ACTACTTGGC TCTGCAAAGC CTGGCTATTA CATCAGTGCA 
GAGGCTCCTT TGGAAAATAA CAAGAAAGTT ATCGTTATGG AAAAAATTCC TGCTGCCACA 
GACTCTAATG GCTCCCATGA TGTGGAAAGC GATGACACTT ACGTACAAAT CCGATCCCCC 
ACAAGCAGAG AGAAGATCTC TCTCAAAGCC GTGATTGAGC GCTGCAAAGC CTACCAGGAG 
TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC TCCAAGGATG GGGAAACCAC TGAATGCACT 
GAATCTGTTC CCCATGAAGA GCCCAATCTG GAGAGGCACG TGGCACCAAG CTGCGATGTC 
AACCAGCAGA ACCGAGTCAA GAACTTGAGA GAAAAGTTTC AGACTAAGCA CAACTCAAGT 
AGTTTGAAAC AATCGGAAGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824565.2
CDS	101..4387
Translation	MFDDRGGRFEGLNRRISRLFESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969111.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969111.3

ORF Insert Sequence:

ATGTTTGATG ATCGTGGGGG ACGCTTCGAA GGGCTTAATC GCAGGATCTC AAGGTTATTT 
GAATCCCCAC GGCTGTCTGC CGCATCCTCA AGCAGCAATG AAAAGCTCTC CATCTCAGCT 
TCAATCTGCA GTGATTCTGA TAGGCCCTTG AGTCTCTTCT CAACAGAGTC CACAGGCTCT 
TTACGTGAAG GCTGTGATTC CTATGAAGAT AACAGCGACG TACCGCTGGG AAACGGAACA 
GTGGTTCACA ACATGGAACT GGTGGCCACT GCAGCAGAGA GCCTGCAGGC CACCTACAGG 
GAGATGCAGA ACGGTCTGAG GAACAACCAC ACACCTAACA ATAATTCTGC CAATCGACGC 
AGCCACCAGG CGTCTAGGTC CCTTTCTCCA TTTGGTAACA AGGTCAATGC CATTACTCAC 
AAACTCAGCT ATGTGGAACG GGTGGTTCTG GAGATCAATG ACACAGAAAG AATGTATGTC 
CAGGATCTGC GCAGCATTGT GGAGGATTAC CTGGGAGCAA TCATAGACAG ACAGGAAGAG 
CTGCCTATAA CCCCAGAGCA AGTCTGTGCT CTCTTTGGAA ACATTGAGGA CATTTATGAA 
CTGAACAGTG AGCTGCTCCA GGACCTGGAT AATTGCAGGA ATGACCCCGT GGCCGTGGCC 
AGCTGCTTTG TCGAAAAGAG TCAGGACTTT GACATCTACA CCCAGTACTG CACCAACTAT 
CCCAACTCCG TGGCCACCTT GACGGAGTGC ATGAGGAATA AAACCCTGGC CAAGTTCTTT 
CGAGAGAAGC AGGAGATGCT GAACCAATCC CTTCCACTCG GCTCCTACCT CCTAAAGCCA 
GTGCAGAGAA TACTGAAGTA TCACCTCCTA TTGCAGGAAA TAGCAAAACA TCTTGATGTG 
AAGGTGGAAG GGTATGAGGT GCTGGAGGAG GCTATTGAAA CCATGACGGG AGTAGCCTGG 
TACATTAACG ACATGAAAAG AAAGCACGAA CATGCTGTGC GGCAGCAGGA GATCCAGTCT 
TTGCTGTTGA ACTGGAAAGG TATAGATCTC ACTGCATATG GAGAACTTGT GCTGGAGGGA 
ACATTTCGGC TCCAGCGAGC ACGGAGTGAG AGGACATTTT TCCTTTTCGA TAAGGCCCTG 
TTTATCACCA AGAGACGTGG GGATCACTAT GTCTACAAAA CTCATATCCC GTGTTCAAGC 
TTGATGCTGA TTGAGAGTGC CCGTGACTCC CTCTGCTTTA GTGTGACCCA TTACAAGAAC 
AGCAAACAGC AGCATAATGT GCAGGCCAAA ACAGTTGAAG AGAAACATGT GTGGACCCAT 
CATATAAAGA AACTCATACT AGAGAACCAC CATGCAATCA TTCCCCAAAA GGCGAAGGAG 
GCAATACTGG AAATGGATAA CATGTATAAA GGAAGGTACA AATACAGCCC AGAGAGGCAA 
AAGAAGACCA TGTCTGCCAT TGAGCCCTCC ATGACACACC GGTCAGGACG CAGACAGTCT 
GAGCCCTCCA AAGACATACT AAAGCAGCTG AATGACAAAG CAGGATTAAA GCATGCAGGC 
AGTGATGGAG ATCTTCAGGA ATTTGGGGAC ATTCCACAGC CTTGTATTGG CAGCTTGGGC 
ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA GACAAGGAGC GTCCTGTCAG CACACAGCTG 
AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC TCTGATGAGG ATGAGGAGGA GATACAGTTG 
GGAGATGATG ATAATGATGA TGATGATGAG CAGACTAAAG ATCCGGTGGA GGAAGAGGCA 
CCAAAGGGCT GCAAGAGACA AAACAGCCAG TCATCTGGGA ATGCAGAGAA GAGGAGGAGT 
GTGGAGGTCA CATCTGGGCA CCAGGAGTCT GTATATAAAA TGGATAAGGA GAATTCAGAG 
CCAGAAGAGA TCTACAAGGC TGTGGTCGTC AGCTCTTCTG AGGATGAACA AGCTGTTCTG 
TTGGAAATCA CAGATGACAC AGAGCCAGCA AAAGATGATA GTGCAGCTCA CCCAGAAGTC 
CTTGAGCCTG TGGAGAATGA AGAAGCTGAA AGTACAGAAG ACTCAAAGAC TTTCAGCAGC 
GAAGAAGAGG AAGAGGAGAC ACCAACCCAT GAATCAACAA GTATCCTACC TCCTTCTGTT 
CTGGACCAGG CCAGTGTTAT TGCTGAGAGG TTTATAAGCA GCATGTCCAG GAGGAGTAGC 
ATGGCTCTAG ATGATGGGAA AGTCATAGGC TACATCACCC CCAGATTAAC CAGCCGCTGC 
AGTAGTTTGA TCAATCTGGA ATCTACAGAT ACTATTATTT ATCAGAACGG TTCAAGCGAC 
TTCCAGGACT TCTCAGAAGT TTGTGGTGAC ACTGGAGTAG ACATTCTTGA CACCACTTTT 
AGTAAGGTGA ACTCTGCCAG TATGGAGAAT GTGTTTGAGG AAAGAGAAAG GCCCACATGT 
AAGAGAAGAG ACTCTATTCT ATCCATGCAA GATAGACAAC TCATTGATAA AATCAGAACT 
TACTATGACG ATGCAGAAAA TCGGGATGCA TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG 
AACTATATTC CCACTGGACT TGTTAGAAAC TCTGTATTTA AGATTAACAG CCTGCCCAGA 
TTTGATACTA CCCAGAGTTC TCTATCGAGG CAGAGAATGT CCAGTTCATC TAGTAATACC 
AGCGGTGTGA ACAGTAGACA TGCTTCCTTG ACAGATACAA GGTCTAATGC AAGAGAATCA 
GAGCAGTTAG AGACGCAAAC TGAACTGCTT TTGGAAACAG CCACAAATGG CACTTCTGAC 
CATTCAGCCC CGATTACTGA TGAGGAGTTC CGGACACCAG ATGAAATGAT TAAAGTCTGG 
GATGAGTTTG AAAAGGTAAA TGCTTGGAAA GAGGCCTCAG ATATTTTAGA AGCCAAAAAA 
GATGTCAAAC CAGACTCTGT GGGTGAAAGT ACAGCACACT GTGCCCTGGA GAATGGCTTG 
GAATCTCACG AACCACTGCT CATATTGGAA GACAGCGATC TGAGTACTAT AACAGAGGAA 
TCGCCTCTTC CTACACCGGA AAGCACATCT CCTAAACAGT TTGGCTGTGT TCCCAATAAC 
ACAAGTTTTA GTGAGTACAA AACGCCTGTG AAGCTACACT CCAAGATAAT GCAAATGGCC 
AGCTGCATGG ATGAAGACAT GTCGGAGAAG ATGAAGAACA AGGTGTACCA GCTGGCCAGG 
CAGTACAGTC AGAGGATCAA ATGCAACAAG CCAGTGTCCC AAAGGCAGCT AAAGGAGATA 
GAGGAGGATT TCAGAAGGAA CAGCCTGCCT GCTGTGCAAG AGGAAAAACT GGATAAGAAA 
GAGAAACGCA AGCCAATACT GTCACTCCCC ACATACGACA GCGTTATACT CCAGGAGCAT 
GGCCCTACAA CCCCAACATC GGCCCTGAGC AGAGACACAG CTCCAAAGCA TCTTTCCTTT 
TCGCCATCCT GTGACAGCCC ACGGGCTCTT TCCCCAGAGA GAGCTGACTG CAGGAGCCCT 
CTGAGTCCAG TCAAGGCAGA GAGATTTCAC TGGCCTGACG TCCGAGAACT GAGATCTAGG 
TACACAAGTG CAGCCAGCTC TAGCAAGGCT CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA 
GAAAGAATAA CTGTGCCCAA TTTGAGGGGG CATTCTGACA CACAAAGTAA GGGTTGCTTG 
ATTCACTCTC ACAGCATGAA CGTGAAACAC ACAGAAAATG TCAATGGCAG CGCTGAGGAC 
TCGGCACAGA ATAACAAAGC ACAGGTAGAA ACTGAGCCGC CTTTGCCAGT GTCACCCAGT 
CTAAAAGGTA AAACTGATTC ACTACTTGGC TCTGCAAAGC CTGGCTATTA CATCAGTGCA 
GAGGCTCCTT TGGAAAATAA CAAGAAAGTT ATCGTTATGG AAAAAATTCC TGCTGCCACA 
GACTCTAATG GCTCCCATGA TGTGGAAAGC GATGACACTT ACGTACAAAT CCGATCCCCC 
ACAAGCAGAG AGAAGATCTC TCTCAAAGCC GTGATTGAGC GCTGCAAAGC CTACCAGGAG 
TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC TCCAAGGATG GGGAAACCAC TGAATGCACT 
GAATCTGTTC CCCATGAAGA GCCCAATCTG GAGAGGCACG TGGCACCAAG CTGCGATGTC 
AACCAGCAGA ACCGAGTCAA GAACTTGAGA GAAAAGTTTC AGACTAAGCA CAACTCAAGT 
AGTTTGAAAC AATCGGAAGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917285.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917342.3 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.

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CloneID	OXa65745
Clone ID Related Accession (Same CDS sequence)	XM_004917285.4
Accession Version	XM_004917285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917285.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGCAGGAT TAAAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917342.3
CDS	40..4218
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917285.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917285.4

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGCAGGAT TAAAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAA GTACAGCACA CTGTGCCCTG 
GAGAATGGCT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TTTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCGCCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAGGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAA GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917286.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917343.3 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.

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CloneID	OXa65746
Clone ID Related Accession (Same CDS sequence)	XM_004917286.4
Accession Version	XM_004917286.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917286.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGGATTAA AGACTAAAGA TCCGGTGGAG 
GAAGAGGCAC CAAAGGGCTG CAAGAGACAA AACAGCCAGT CATCTGGGAA TGCAGAGAAG 
AGGAGGAGTG TGGAGGTCAC ATCTGGGCAC CAGGAGTCTG TATATAAAAT GGATAAGGAG 
AATTCAGAGC CAGAAGAGAT CTACAAGGCT GTGGTCGTCA GCTCTTCTGA GGATGAACAA 
GCTGTTCTGT TGGAAATCAC AGATGACACA GAGCCAGCAA AAGATGATAG TGCAGCTCAC 
CCAGAAGTCC TTGAGCCTGT GGAGAATGAA GAAGCTGAAA GTACAGAAGA CTCAAAGACT 
TTCAGCAGCG AAGAAGAGGA AGAGGAGACA CCAACCCATG AATCAACAAG TATCCTACCT 
CCTTCTGTTC TGGACCAGGC CAGTGTTATT GCTGAGAGGT TTATAAGCAG CATGTCCAGG 
AGGAGTAGCA TGGCTCTAGA TGATGGGAAA GTCATAGGCT ACATCACCCC CAGATTAACC 
AGCCGCTGCA GTAGTTTGAT CAATCTGGAA TCTACAGATA CTATTATTTA TCAGAACGGT 
TCAAGCGACT TCCAGGACTT CTCAGAAGTT TGTGGTGACA CTGGAGTAGA CATTCTTGAC 
ACCACTTTTA GTAAGGTGAA CTCTGCCAGT ATGGAGAATG TGTTTGAGGA AAGAGAAAGG 
CCCACATGTA AGAGAAGAGA CTCTATTCTA TCCATGCAAG ATAGACAACT CATTGATAAA 
ATCAGAACTT ACTATGACGA TGCAGAAAAT CGGGATGCAT CCTTTAGCAT TAAGCGCAGA 
GAGAGCTTGA ACTATATTCC CACTGGACTT GTTAGAAACT CTGTATTTAA GATTAACAGC 
CTGCCCAGAT TTGATACTAC CCAGAGTTCT CTATCGAGGC AGAGAATGTC CAGTTCATCT 
AGTAATACCA GCGGTGTGAA CAGTAGACAT GCTTCCTTGA CAGATACAAG GTCTAATGCA 
AGAGAATCAG AGCAGTTAGA GACGCAAACT GAACTGCTTT TGGAAACAGC CACAAATGGC 
ACTTCTGACC ATTCAGCCCC GATTACTGAT GAGGAGTTCC GGACACCAGA TGAAATGATT 
AAAGTCTGGG ATGAGTTTGA AAAGGTAAAT GCTTGGAAAG AGGCCTCAGA TATTTTAGAA 
GCCAAAAAAG ATGTCAAACC AGACTCTGTG GGTGAAAGTA CAGCACACTG TGCCCTGGAG 
AATGGCTTGG AATCTCACGA ACCACTGCTC ATATTGGAAG ACAGCGATCT GAGTACTATA 
ACAGAGGAAT CGCCTCTTCC TACACCGGAA AGCACATCTC CTAAACAGTT TGGCTGTGTT 
CCCAATAACA CAAGTTTTAG TGAGTACAAA ACGCCTGTGA AGCTACACTC CAAGATAATG 
CAAATGGCCA GCTGCATGGA TGAAGACATG TCGGAGAAGA TGAAGAACAA GGTGTACCAG 
CTGGCCAGGC AGTACAGTCA GAGGATCAAA TGCAACAAGC CAGTGTCCCA AAGGCAGCTA 
AAGGAGATAG AGGAGGATTT CAGAAGGAAC AGCCTGCCTG CTGTGCAAGA GGAAAAACTG 
GATAAGAAAG AGAAACGCAA GCCAATACTG TCACTCCCCA CATACGACAG CGTTATACTC 
CAGGAGCATG GCCCTACAAC CCCAACATCG GCCCTGAGCA GAGACACAGC TCCAAAGCAT 
CTTTCCTTTT CGCCATCCTG TGACAGCCCA CGGGCTCTTT CCCCAGAGAG AGCTGACTGC 
AGGAGCCCTC TGAGTCCAGT CAAGGCAGAG AGATTTCACT GGCCTGACGT CCGAGAACTG 
AGATCTAGGT ACACAAGTGC AGCCAGCTCT AGCAAGGCTC CTCCCGTCAA CAGGAGCCAG 
TCTGTGCCAG AAAGAATAAC TGTGCCCAAT TTGAGGGGGC ATTCTGACAC ACAAAGTAAG 
GGTTGCTTGA TTCACTCTCA CAGCATGAAC GTGAAACACA CAGAAAATGT CAATGGCAGC 
GCTGAGGACT CGGCACAGAA TAACAAAGCA CAGGTAGAAA CTGAGCCGCC TTTGCCAGTG 
TCACCCAGTC TAAAAGGTAA AACTGATTCA CTACTTGGCT CTGCAAAGCC TGGCTATTAC 
ATCAGTGCAG AGGCTCCTTT GGAAAATAAC AAGAAAGTTA TCGTTATGGA AAAAATTCCT 
GCTGCCACAG ACTCTAATGG CTCCCATGAT GTGGAAAGCG ATGACACTTA CGTACAAATC 
CGATCCCCCA CAAGCAGAGA GAAGATCTCT CTCAAAGCCG TGATTGAGCG CTGCAAAGCC 
TACCAGGAGT CCGAGGAGTA CCGGTCCAGG GAAGTAGCCT CCAAGGATGG GGAAACCACT 
GAATGCACTG AATCTGTTCC CCATGAAGAG CCCAATCTGG AGAGGCACGT GGCACCAAGC 
TGCGATGTCA ACCAGCAGAA CCGAGTCAAG AACTTGAGAG AAAAGTTTCA GACTAAGCAC 
AACTCAAGTA GTTTGAAACA ATCGGAAGTT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917343.3
CDS	40..4215
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKGLKTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917286.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917286.4

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCT AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA GCTGAATGAC AAAGGATTAA AGACTAAAGA TCCGGTGGAG 
GAAGAGGCAC CAAAGGGCTG CAAGAGACAA AACAGCCAGT CATCTGGGAA TGCAGAGAAG 
AGGAGGAGTG TGGAGGTCAC ATCTGGGCAC CAGGAGTCTG TATATAAAAT GGATAAGGAG 
AATTCAGAGC CAGAAGAGAT CTACAAGGCT GTGGTCGTCA GCTCTTCTGA GGATGAACAA 
GCTGTTCTGT TGGAAATCAC AGATGACACA GAGCCAGCAA AAGATGATAG TGCAGCTCAC 
CCAGAAGTCC TTGAGCCTGT GGAGAATGAA GAAGCTGAAA GTACAGAAGA CTCAAAGACT 
TTCAGCAGCG AAGAAGAGGA AGAGGAGACA CCAACCCATG AATCAACAAG TATCCTACCT 
CCTTCTGTTC TGGACCAGGC CAGTGTTATT GCTGAGAGGT TTATAAGCAG CATGTCCAGG 
AGGAGTAGCA TGGCTCTAGA TGATGGGAAA GTCATAGGCT ACATCACCCC CAGATTAACC 
AGCCGCTGCA GTAGTTTGAT CAATCTGGAA TCTACAGATA CTATTATTTA TCAGAACGGT 
TCAAGCGACT TCCAGGACTT CTCAGAAGTT TGTGGTGACA CTGGAGTAGA CATTCTTGAC 
ACCACTTTTA GTAAGGTGAA CTCTGCCAGT ATGGAGAATG TGTTTGAGGA AAGAGAAAGG 
CCCACATGTA AGAGAAGAGA CTCTATTCTA TCCATGCAAG ATAGACAACT CATTGATAAA 
ATCAGAACTT ACTATGACGA TGCAGAAAAT CGGGATGCAT CCTTTAGCAT TAAGCGCAGA 
GAGAGCTTGA ACTATATTCC CACTGGACTT GTTAGAAACT CTGTATTTAA GATTAACAGC 
CTGCCCAGAT TTGATACTAC CCAGAGTTCT CTATCGAGGC AGAGAATGTC CAGTTCATCT 
AGTAATACCA GCGGTGTGAA CAGTAGACAT GCTTCCTTGA CAGATACAAG GTCTAATGCA 
AGAGAATCAG AGCAGTTAGA GACGCAAACT GAACTGCTTT TGGAAACAGC CACAAATGGC 
ACTTCTGACC ATTCAGCCCC GATTACTGAT GAGGAGTTCC GGACACCAGA TGAAATGATT 
AAAGTCTGGG ATGAGTTTGA AAAGGTAAAT GCTTGGAAAG AGGCCTCAGA TATTTTAGAA 
GCCAAAAAAG ATGTCAAACC AGACTCTGTG GGTGAAAGTA CAGCACACTG TGCCCTGGAG 
AATGGCTTGG AATCTCACGA ACCACTGCTC ATATTGGAAG ACAGCGATCT GAGTACTATA 
ACAGAGGAAT CGCCTCTTCC TACACCGGAA AGCACATCTC CTAAACAGTT TGGCTGTGTT 
CCCAATAACA CAAGTTTTAG TGAGTACAAA ACGCCTGTGA AGCTACACTC CAAGATAATG 
CAAATGGCCA GCTGCATGGA TGAAGACATG TCGGAGAAGA TGAAGAACAA GGTGTACCAG 
CTGGCCAGGC AGTACAGTCA GAGGATCAAA TGCAACAAGC CAGTGTCCCA AAGGCAGCTA 
AAGGAGATAG AGGAGGATTT CAGAAGGAAC AGCCTGCCTG CTGTGCAAGA GGAAAAACTG 
GATAAGAAAG AGAAACGCAA GCCAATACTG TCACTCCCCA CATACGACAG CGTTATACTC 
CAGGAGCATG GCCCTACAAC CCCAACATCG GCCCTGAGCA GAGACACAGC TCCAAAGCAT 
CTTTCCTTTT CGCCATCCTG TGACAGCCCA CGGGCTCTTT CCCCAGAGAG AGCTGACTGC 
AGGAGCCCTC TGAGTCCAGT CAAGGCAGAG AGATTTCACT GGCCTGACGT CCGAGAACTG 
AGATCTAGGT ACACAAGTGC AGCCAGCTCT AGCAAGGCTC CTCCCGTCAA CAGGAGCCAG 
TCTGTGCCAG AAAGAATAAC TGTGCCCAAT TTGAGGGGGC ATTCTGACAC ACAAAGTAAG 
GGTTGCTTGA TTCACTCTCA CAGCATGAAC GTGAAACACA CAGAAAATGT CAATGGCAGC 
GCTGAGGACT CGGCACAGAA TAACAAAGCA CAGGTAGAAA CTGAGCCGCC TTTGCCAGTG 
TCACCCAGTC TAAAAGGTAA AACTGATTCA CTACTTGGCT CTGCAAAGCC TGGCTATTAC 
ATCAGTGCAG AGGCTCCTTT GGAAAATAAC AAGAAAGTTA TCGTTATGGA AAAAATTCCT 
GCTGCCACAG ACTCTAATGG CTCCCATGAT GTGGAAAGCG ATGACACTTA CGTACAAATC 
CGATCCCCCA CAAGCAGAGA GAAGATCTCT CTCAAAGCCG TGATTGAGCG CTGCAAAGCC 
TACCAGGAGT CCGAGGAGTA CCGGTCCAGG GAAGTAGCCT CCAAGGATGG GGAAACCACT 
GAATGCACTG AATCTGTTCC CCATGAAGAG CCCAATCTGG AGAGGCACGT GGCACCAAGC 
TGCGATGTCA ACCAGCAGAA CCGAGTCAAG AACTTGAGAG AAAAGTTTCA GACTAAGCAC 
AACTCAAGTA GTTTGAAACA ATCGGAAGTT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969112.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824566.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.

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CloneID	OXa65747
Clone ID Related Accession (Same CDS sequence)	XM_012969112.3
Accession Version	XM_012969112.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCTAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAGCTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAAAGTAC AGCACACTGT GCCCTGGAGA ATGGCTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATTTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC GCCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAGG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAAGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824566.2
CDS	405..4439
Translation	MELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGESTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDFRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969112.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969112.3

ORF Insert Sequence:

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCTAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAGCTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAAAGTAC AGCACACTGT GCCCTGGAGA ATGGCTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATTTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC GCCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAGG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAAGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917284.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917341.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa36859
Clone ID Related Accession (Same CDS sequence)	XM_004917284.3
Accession Version	XM_004917284.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917284.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGCAGGAT TAAAGCATGC AGGCAGTGAT 
GGAGATCTTC AGGAATTTGG GGACATTCCA CAGCCTTGTA TTGGCAGCTT GGGCACCAGC 
CTTGAGCAGC CAGAGGCTGA GGGAGACAAG GAGCGTCCTG TCAGCACACA GCTGAGTAGA 
AGCTCAGTGG CAGAGCTCAG TGCCTCTGAT GAGGATGAGG AGGAGATACA GTTGGGAGAT 
GATGATAATG ATGATGATGA TGAGCAGACT AAAGATCCGG TGGAGGAAGA GGCACCAAAG 
GGCTGCAAGA GACAAAACAG CCAGTCATCT GGGAATGCAG AGAAGAGGAG GAGTGTGGAG 
GTCACATCTG GGCACCAGGA GTCTGTATAT AAAATGGATA AGGAGAATTC AGAGCCAGAA 
GAGATCTACA AGGCTGTGGT CGTCAGCTCT TCTGAGGATG AACAAGCTGT TCTGTTGGAA 
ATCACAGATG ACACAGAGCC AGCAAAAGAT GATAGTGCAG CTCACCCAGA AGTCCTTGAG 
CCTGTGGAGA ATGAAGAAGC TGAAAGTACA GAAGACTCAA AGACTTTCAG CAGCGAAGAA 
GAGGAAGAGG AGACACCAAC CCATGAATCA ACAAGTATCC TACCTCCTTC TGTTCTGGAC 
CAGGCCAGTG TTATTGCTGA GAGGTTTATA AGCAGCATGT CCAGGAGGAG TAGCATGGCT 
CTAGATGATG GGAAAGTCAT AGGCTACATC ACCCCCAGAT TAACCAGCCG CTGCAGTAGT 
TTGATCAATC TGGAATCTAC AGATACTATT ATTTATCAGA ACGGTTCAAG CGACTTCCAG 
GACTTCTCAG AAGTTTGTGG TGACACTGGA GTAGACATTC TTGACACCAC TTTTAGTAAG 
GTGAACTCTG CCAGTATGGA GAATGTGTTT GAGGAAAGAG AAAGGCCCAC ATGTAAGAGA 
AGAGACTCTA TTCTATCCAT GCAAGATAGA CAACTCATTG ATAAAATCAG AACTTACTAT 
GACGATGCAG AAAATCGGGA TGCATCCTTT AGCATTAAGC GCAGAGAGAG CTTGAACTAT 
ATTCCCACTG GACTTGTTAG AAACTCTGTA TTTAAGATTA ACAGCCTGCC CAGATTTGAT 
ACTACCCAGA GTTCTCTATC GAGGCAGAGA ATGTCCAGTT CATCTAGTAA TACCAGCGGT 
GTGAACAGTA GACATGCTTC CTTGACAGAT ACAAGGTCTA ATGCAAGAGA ATCAGAGCAG 
TTAGAGACGC AAACTGAACT GCTTTTGGAA ACAGCCACAA ATGGCACTTC TGACCATTCA 
GCCCCGATTA CTGATGAGGA GTTCCGGACA CCAGATGAAA TGATTAAAGT CTGGGATGAG 
TTTGAAAAGG TAAATGCTTG GAAAGAGGCC TCAGATATTT TAGAAGCCAA AAAAGATGTC 
AAACCAGACT CTGTGGGTGA ATGTACAGCA CACTGTGCCC TGGAGAATGG TTTGGAATCT 
CACGAACCAC TGCTCATATT GGAAGACAGC GATCTGAGTA CTATAACAGA GGAATCGCCT 
CTTCCTACAC CGGAAAGCAC ATCTCCTAAA CAGTTTGGCT GTGTTCCCAA TAACACAAGT 
TTTAGTGAGT ACAAAACGCC TGTGAAGCTA CACTCCAAGA TAATGCAAAT GGCCAGCTGC 
ATGGATGAAG ACATGTCGGA GAAGATGAAG AACAAGGTGT ACCAGCTGGC CAGGCAGTAC 
AGTCAGAGGA TCAAATGCAA CAAGCCAGTG TCCCAAAGGC AGCTAAAGGA GATAGAGGAG 
GATCTCAGAA GGAACAGCCT GCCTGCTGTG CAAGAGGAAA AACTGGATAA GAAAGAGAAA 
CGCAAGCCAA TACTGTCACT CCCCACATAC GACAGCGTTA TACTCCAGGA GCATGGCCCT 
ACAACCCCAA CATCGGCCCT GAGCAGAGAC ACAGCTCCAA AGCATCTTTC CTTTTCACCA 
TCCTGTGACA GCCCACGGGC TCTTTCCCCA GAGAGAGCTG ACTGCAGGAG CCCTCTGAGT 
CCAGTCAAGG CAGAGAGATT TCACTGGCCT GACGTCCGAG AACTGAGATC TAGGTACACA 
AGTGCAGCCA GCTCTAGCAA GGCTCCTCCC GTCAACAGGA GCCAGTCTGT GCCAGAAAGA 
ATAACTGTGC CCAATTTGAG GGGGCATTCT GACACACAAA GTAAAGGTTG CTTGATTCAC 
TCTCACAGCA TGAACGTGAA ACACACAGAA AATGTCAATG GCAGCGCTGA GGACTCGGCA 
CAGAATAACA AAGCACAGGT AGAAACTGAG CCGCCTTTGC CAGTGTCACC CAGTCTAAAA 
GGTAAAACTG ATTCACTACT TGGCTCTGCA AAGCCTGGCT ATTACATCAG TGCAGAGGCT 
CCTTTGGAAA ATAACAAGAA AGTTATCGTT ATGGAAAAAA TTCCTGCTGC CACAGACTCT 
AATGGCTCCC ATGATGTGGA AAGCGATGAC ACTTACGTAC AAATCCGATC CCCCACAAGC 
AGAGAGAAGA TCTCTCTCAA AGCCGTGATT GAGCGCTGCA AAGCCTACCA GGAGTCCGAG 
GAGTACCGGT CCAGGGAAGT AGCCTCCAAG GATGGGGAAA CCACTGAATG CACTGAATCT 
GTTCCCCATG AAGAGCCCAA TCTGGAGAGG CACGTGGCAC CAAGCTGCGA TGTCAACCAG 
CAGAACCGAG TCAAGAACTT GAGAGAAAAG TTTCAGACTA AGCACAACTC AAGTAGTTTG 
AAACAATCGG AGGTTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917341.2
CDS	59..4459
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917284.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917284.3

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGCAGGAT TAAAGCATGC AGGCAGTGAT 
GGAGATCTTC AGGAATTTGG GGACATTCCA CAGCCTTGTA TTGGCAGCTT GGGCACCAGC 
CTTGAGCAGC CAGAGGCTGA GGGAGACAAG GAGCGTCCTG TCAGCACACA GCTGAGTAGA 
AGCTCAGTGG CAGAGCTCAG TGCCTCTGAT GAGGATGAGG AGGAGATACA GTTGGGAGAT 
GATGATAATG ATGATGATGA TGAGCAGACT AAAGATCCGG TGGAGGAAGA GGCACCAAAG 
GGCTGCAAGA GACAAAACAG CCAGTCATCT GGGAATGCAG AGAAGAGGAG GAGTGTGGAG 
GTCACATCTG GGCACCAGGA GTCTGTATAT AAAATGGATA AGGAGAATTC AGAGCCAGAA 
GAGATCTACA AGGCTGTGGT CGTCAGCTCT TCTGAGGATG AACAAGCTGT TCTGTTGGAA 
ATCACAGATG ACACAGAGCC AGCAAAAGAT GATAGTGCAG CTCACCCAGA AGTCCTTGAG 
CCTGTGGAGA ATGAAGAAGC TGAAAGTACA GAAGACTCAA AGACTTTCAG CAGCGAAGAA 
GAGGAAGAGG AGACACCAAC CCATGAATCA ACAAGTATCC TACCTCCTTC TGTTCTGGAC 
CAGGCCAGTG TTATTGCTGA GAGGTTTATA AGCAGCATGT CCAGGAGGAG TAGCATGGCT 
CTAGATGATG GGAAAGTCAT AGGCTACATC ACCCCCAGAT TAACCAGCCG CTGCAGTAGT 
TTGATCAATC TGGAATCTAC AGATACTATT ATTTATCAGA ACGGTTCAAG CGACTTCCAG 
GACTTCTCAG AAGTTTGTGG TGACACTGGA GTAGACATTC TTGACACCAC TTTTAGTAAG 
GTGAACTCTG CCAGTATGGA GAATGTGTTT GAGGAAAGAG AAAGGCCCAC ATGTAAGAGA 
AGAGACTCTA TTCTATCCAT GCAAGATAGA CAACTCATTG ATAAAATCAG AACTTACTAT 
GACGATGCAG AAAATCGGGA TGCATCCTTT AGCATTAAGC GCAGAGAGAG CTTGAACTAT 
ATTCCCACTG GACTTGTTAG AAACTCTGTA TTTAAGATTA ACAGCCTGCC CAGATTTGAT 
ACTACCCAGA GTTCTCTATC GAGGCAGAGA ATGTCCAGTT CATCTAGTAA TACCAGCGGT 
GTGAACAGTA GACATGCTTC CTTGACAGAT ACAAGGTCTA ATGCAAGAGA ATCAGAGCAG 
TTAGAGACGC AAACTGAACT GCTTTTGGAA ACAGCCACAA ATGGCACTTC TGACCATTCA 
GCCCCGATTA CTGATGAGGA GTTCCGGACA CCAGATGAAA TGATTAAAGT CTGGGATGAG 
TTTGAAAAGG TAAATGCTTG GAAAGAGGCC TCAGATATTT TAGAAGCCAA AAAAGATGTC 
AAACCAGACT CTGTGGGTGA ATGTACAGCA CACTGTGCCC TGGAGAATGG TTTGGAATCT 
CACGAACCAC TGCTCATATT GGAAGACAGC GATCTGAGTA CTATAACAGA GGAATCGCCT 
CTTCCTACAC CGGAAAGCAC ATCTCCTAAA CAGTTTGGCT GTGTTCCCAA TAACACAAGT 
TTTAGTGAGT ACAAAACGCC TGTGAAGCTA CACTCCAAGA TAATGCAAAT GGCCAGCTGC 
ATGGATGAAG ACATGTCGGA GAAGATGAAG AACAAGGTGT ACCAGCTGGC CAGGCAGTAC 
AGTCAGAGGA TCAAATGCAA CAAGCCAGTG TCCCAAAGGC AGCTAAAGGA GATAGAGGAG 
GATCTCAGAA GGAACAGCCT GCCTGCTGTG CAAGAGGAAA AACTGGATAA GAAAGAGAAA 
CGCAAGCCAA TACTGTCACT CCCCACATAC GACAGCGTTA TACTCCAGGA GCATGGCCCT 
ACAACCCCAA CATCGGCCCT GAGCAGAGAC ACAGCTCCAA AGCATCTTTC CTTTTCACCA 
TCCTGTGACA GCCCACGGGC TCTTTCCCCA GAGAGAGCTG ACTGCAGGAG CCCTCTGAGT 
CCAGTCAAGG CAGAGAGATT TCACTGGCCT GACGTCCGAG AACTGAGATC TAGGTACACA 
AGTGCAGCCA GCTCTAGCAA GGCTCCTCCC GTCAACAGGA GCCAGTCTGT GCCAGAAAGA 
ATAACTGTGC CCAATTTGAG GGGGCATTCT GACACACAAA GTAAAGGTTG CTTGATTCAC 
TCTCACAGCA TGAACGTGAA ACACACAGAA AATGTCAATG GCAGCGCTGA GGACTCGGCA 
CAGAATAACA AAGCACAGGT AGAAACTGAG CCGCCTTTGC CAGTGTCACC CAGTCTAAAA 
GGTAAAACTG ATTCACTACT TGGCTCTGCA AAGCCTGGCT ATTACATCAG TGCAGAGGCT 
CCTTTGGAAA ATAACAAGAA AGTTATCGTT ATGGAAAAAA TTCCTGCTGC CACAGACTCT 
AATGGCTCCC ATGATGTGGA AAGCGATGAC ACTTACGTAC AAATCCGATC CCCCACAAGC 
AGAGAGAAGA TCTCTCTCAA AGCCGTGATT GAGCGCTGCA AAGCCTACCA GGAGTCCGAG 
GAGTACCGGT CCAGGGAAGT AGCCTCCAAG GATGGGGAAA CCACTGAATG CACTGAATCT 
GTTCCCCATG AAGAGCCCAA TCTGGAGAGG CACGTGGCAC CAAGCTGCGA TGTCAACCAG 
CAGAACCGAG TCAAGAACTT GAGAGAAAAG TTTCAGACTA AGCACAACTC AAGTAGTTTG 
AAACAATCGG AGGTTCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969107.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824561.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.

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CloneID	OXa36860
Clone ID Related Accession (Same CDS sequence)	XM_012969107.2
Accession Version	XM_012969107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGGATTAA AGCATGCAGG CAGTGATGGA 
GATCTTCAGG AATTTGGGGA CATTCCACAG CCTTGTATTG GCAGCTTGGG CACCAGCCTT 
GAGCAGCCAG AGGCTGAGGG AGACAAGGAG CGTCCTGTCA GCACACAGCT GAGTAGAAGC 
TCAGTGGCAG AGCTCAGTGC CTCTGATGAG GATGAGGAGG AGATACAGTT GGGAGATGAT 
GATAATGATG ATGATGATGA GCAGACTAAA GATCCGGTGG AGGAAGAGGC ACCAAAGGGC 
TGCAAGAGAC AAAACAGCCA GTCATCTGGG AATGCAGAGA AGAGGAGGAG TGTGGAGGTC 
ACATCTGGGC ACCAGGAGTC TGTATATAAA ATGGATAAGG AGAATTCAGA GCCAGAAGAG 
ATCTACAAGG CTGTGGTCGT CAGCTCTTCT GAGGATGAAC AAGCTGTTCT GTTGGAAATC 
ACAGATGACA CAGAGCCAGC AAAAGATGAT AGTGCAGCTC ACCCAGAAGT CCTTGAGCCT 
GTGGAGAATG AAGAAGCTGA AAGTACAGAA GACTCAAAGA CTTTCAGCAG CGAAGAAGAG 
GAAGAGGAGA CACCAACCCA TGAATCAACA AGTATCCTAC CTCCTTCTGT TCTGGACCAG 
GCCAGTGTTA TTGCTGAGAG GTTTATAAGC AGCATGTCCA GGAGGAGTAG CATGGCTCTA 
GATGATGGGA AAGTCATAGG CTACATCACC CCCAGATTAA CCAGCCGCTG CAGTAGTTTG 
ATCAATCTGG AATCTACAGA TACTATTATT TATCAGAACG GTTCAAGCGA CTTCCAGGAC 
TTCTCAGAAG TTTGTGGTGA CACTGGAGTA GACATTCTTG ACACCACTTT TAGTAAGGTG 
AACTCTGCCA GTATGGAGAA TGTGTTTGAG GAAAGAGAAA GGCCCACATG TAAGAGAAGA 
GACTCTATTC TATCCATGCA AGATAGACAA CTCATTGATA AAATCAGAAC TTACTATGAC 
GATGCAGAAA ATCGGGATGC ATCCTTTAGC ATTAAGCGCA GAGAGAGCTT GAACTATATT 
CCCACTGGAC TTGTTAGAAA CTCTGTATTT AAGATTAACA GCCTGCCCAG ATTTGATACT 
ACCCAGAGTT CTCTATCGAG GCAGAGAATG TCCAGTTCAT CTAGTAATAC CAGCGGTGTG 
AACAGTAGAC ATGCTTCCTT GACAGATACA AGGTCTAATG CAAGAGAATC AGAGCAGTTA 
GAGACGCAAA CTGAACTGCT TTTGGAAACA GCCACAAATG GCACTTCTGA CCATTCAGCC 
CCGATTACTG ATGAGGAGTT CCGGACACCA GATGAAATGA TTAAAGTCTG GGATGAGTTT 
GAAAAGGTAA ATGCTTGGAA AGAGGCCTCA GATATTTTAG AAGCCAAAAA AGATGTCAAA 
CCAGACTCTG TGGGTGAATG TACAGCACAC TGTGCCCTGG AGAATGGTTT GGAATCTCAC 
GAACCACTGC TCATATTGGA AGACAGCGAT CTGAGTACTA TAACAGAGGA ATCGCCTCTT 
CCTACACCGG AAAGCACATC TCCTAAACAG TTTGGCTGTG TTCCCAATAA CACAAGTTTT 
AGTGAGTACA AAACGCCTGT GAAGCTACAC TCCAAGATAA TGCAAATGGC CAGCTGCATG 
GATGAAGACA TGTCGGAGAA GATGAAGAAC AAGGTGTACC AGCTGGCCAG GCAGTACAGT 
CAGAGGATCA AATGCAACAA GCCAGTGTCC CAAAGGCAGC TAAAGGAGAT AGAGGAGGAT 
CTCAGAAGGA ACAGCCTGCC TGCTGTGCAA GAGGAAAAAC TGGATAAGAA AGAGAAACGC 
AAGCCAATAC TGTCACTCCC CACATACGAC AGCGTTATAC TCCAGGAGCA TGGCCCTACA 
ACCCCAACAT CGGCCCTGAG CAGAGACACA GCTCCAAAGC ATCTTTCCTT TTCACCATCC 
TGTGACAGCC CACGGGCTCT TTCCCCAGAG AGAGCTGACT GCAGGAGCCC TCTGAGTCCA 
GTCAAGGCAG AGAGATTTCA CTGGCCTGAC GTCCGAGAAC TGAGATCTAG GTACACAAGT 
GCAGCCAGCT CTAGCAAGGC TCCTCCCGTC AACAGGAGCC AGTCTGTGCC AGAAAGAATA 
ACTGTGCCCA ATTTGAGGGG GCATTCTGAC ACACAAAGTA AAGGTTGCTT GATTCACTCT 
CACAGCATGA ACGTGAAACA CACAGAAAAT GTCAATGGCA GCGCTGAGGA CTCGGCACAG 
AATAACAAAG CACAGGTAGA AACTGAGCCG CCTTTGCCAG TGTCACCCAG TCTAAAAGGT 
AAAACTGATT CACTACTTGG CTCTGCAAAG CCTGGCTATT ACATCAGTGC AGAGGCTCCT 
TTGGAAAATA ACAAGAAAGT TATCGTTATG GAAAAAATTC CTGCTGCCAC AGACTCTAAT 
GGCTCCCATG ATGTGGAAAG CGATGACACT TACGTACAAA TCCGATCCCC CACAAGCAGA 
GAGAAGATCT CTCTCAAAGC CGTGATTGAG CGCTGCAAAG CCTACCAGGA GTCCGAGGAG 
TACCGGTCCA GGGAAGTAGC CTCCAAGGAT GGGGAAACCA CTGAATGCAC TGAATCTGTT 
CCCCATGAAG AGCCCAATCT GGAGAGGCAC GTGGCACCAA GCTGCGATGT CAACCAGCAG 
AACCGAGTCA AGAACTTGAG AGAAAAGTTT CAGACTAAGC ACAACTCAAG TAGTTTGAAA 
CAATCGGAGG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824561.1
CDS	59..4456
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969107.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969107.2

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGGATTAA AGCATGCAGG CAGTGATGGA 
GATCTTCAGG AATTTGGGGA CATTCCACAG CCTTGTATTG GCAGCTTGGG CACCAGCCTT 
GAGCAGCCAG AGGCTGAGGG AGACAAGGAG CGTCCTGTCA GCACACAGCT GAGTAGAAGC 
TCAGTGGCAG AGCTCAGTGC CTCTGATGAG GATGAGGAGG AGATACAGTT GGGAGATGAT 
GATAATGATG ATGATGATGA GCAGACTAAA GATCCGGTGG AGGAAGAGGC ACCAAAGGGC 
TGCAAGAGAC AAAACAGCCA GTCATCTGGG AATGCAGAGA AGAGGAGGAG TGTGGAGGTC 
ACATCTGGGC ACCAGGAGTC TGTATATAAA ATGGATAAGG AGAATTCAGA GCCAGAAGAG 
ATCTACAAGG CTGTGGTCGT CAGCTCTTCT GAGGATGAAC AAGCTGTTCT GTTGGAAATC 
ACAGATGACA CAGAGCCAGC AAAAGATGAT AGTGCAGCTC ACCCAGAAGT CCTTGAGCCT 
GTGGAGAATG AAGAAGCTGA AAGTACAGAA GACTCAAAGA CTTTCAGCAG CGAAGAAGAG 
GAAGAGGAGA CACCAACCCA TGAATCAACA AGTATCCTAC CTCCTTCTGT TCTGGACCAG 
GCCAGTGTTA TTGCTGAGAG GTTTATAAGC AGCATGTCCA GGAGGAGTAG CATGGCTCTA 
GATGATGGGA AAGTCATAGG CTACATCACC CCCAGATTAA CCAGCCGCTG CAGTAGTTTG 
ATCAATCTGG AATCTACAGA TACTATTATT TATCAGAACG GTTCAAGCGA CTTCCAGGAC 
TTCTCAGAAG TTTGTGGTGA CACTGGAGTA GACATTCTTG ACACCACTTT TAGTAAGGTG 
AACTCTGCCA GTATGGAGAA TGTGTTTGAG GAAAGAGAAA GGCCCACATG TAAGAGAAGA 
GACTCTATTC TATCCATGCA AGATAGACAA CTCATTGATA AAATCAGAAC TTACTATGAC 
GATGCAGAAA ATCGGGATGC ATCCTTTAGC ATTAAGCGCA GAGAGAGCTT GAACTATATT 
CCCACTGGAC TTGTTAGAAA CTCTGTATTT AAGATTAACA GCCTGCCCAG ATTTGATACT 
ACCCAGAGTT CTCTATCGAG GCAGAGAATG TCCAGTTCAT CTAGTAATAC CAGCGGTGTG 
AACAGTAGAC ATGCTTCCTT GACAGATACA AGGTCTAATG CAAGAGAATC AGAGCAGTTA 
GAGACGCAAA CTGAACTGCT TTTGGAAACA GCCACAAATG GCACTTCTGA CCATTCAGCC 
CCGATTACTG ATGAGGAGTT CCGGACACCA GATGAAATGA TTAAAGTCTG GGATGAGTTT 
GAAAAGGTAA ATGCTTGGAA AGAGGCCTCA GATATTTTAG AAGCCAAAAA AGATGTCAAA 
CCAGACTCTG TGGGTGAATG TACAGCACAC TGTGCCCTGG AGAATGGTTT GGAATCTCAC 
GAACCACTGC TCATATTGGA AGACAGCGAT CTGAGTACTA TAACAGAGGA ATCGCCTCTT 
CCTACACCGG AAAGCACATC TCCTAAACAG TTTGGCTGTG TTCCCAATAA CACAAGTTTT 
AGTGAGTACA AAACGCCTGT GAAGCTACAC TCCAAGATAA TGCAAATGGC CAGCTGCATG 
GATGAAGACA TGTCGGAGAA GATGAAGAAC AAGGTGTACC AGCTGGCCAG GCAGTACAGT 
CAGAGGATCA AATGCAACAA GCCAGTGTCC CAAAGGCAGC TAAAGGAGAT AGAGGAGGAT 
CTCAGAAGGA ACAGCCTGCC TGCTGTGCAA GAGGAAAAAC TGGATAAGAA AGAGAAACGC 
AAGCCAATAC TGTCACTCCC CACATACGAC AGCGTTATAC TCCAGGAGCA TGGCCCTACA 
ACCCCAACAT CGGCCCTGAG CAGAGACACA GCTCCAAAGC ATCTTTCCTT TTCACCATCC 
TGTGACAGCC CACGGGCTCT TTCCCCAGAG AGAGCTGACT GCAGGAGCCC TCTGAGTCCA 
GTCAAGGCAG AGAGATTTCA CTGGCCTGAC GTCCGAGAAC TGAGATCTAG GTACACAAGT 
GCAGCCAGCT CTAGCAAGGC TCCTCCCGTC AACAGGAGCC AGTCTGTGCC AGAAAGAATA 
ACTGTGCCCA ATTTGAGGGG GCATTCTGAC ACACAAAGTA AAGGTTGCTT GATTCACTCT 
CACAGCATGA ACGTGAAACA CACAGAAAAT GTCAATGGCA GCGCTGAGGA CTCGGCACAG 
AATAACAAAG CACAGGTAGA AACTGAGCCG CCTTTGCCAG TGTCACCCAG TCTAAAAGGT 
AAAACTGATT CACTACTTGG CTCTGCAAAG CCTGGCTATT ACATCAGTGC AGAGGCTCCT 
TTGGAAAATA ACAAGAAAGT TATCGTTATG GAAAAAATTC CTGCTGCCAC AGACTCTAAT 
GGCTCCCATG ATGTGGAAAG CGATGACACT TACGTACAAA TCCGATCCCC CACAAGCAGA 
GAGAAGATCT CTCTCAAAGC CGTGATTGAG CGCTGCAAAG CCTACCAGGA GTCCGAGGAG 
TACCGGTCCA GGGAAGTAGC CTCCAAGGAT GGGGAAACCA CTGAATGCAC TGAATCTGTT 
CCCCATGAAG AGCCCAATCT GGAGAGGCAC GTGGCACCAA GCTGCGATGT CAACCAGCAG 
AACCGAGTCA AGAACTTGAG AGAAAAGTTT CAGACTAAGC ACAACTCAAG TAGTTTGAAA 
CAATCGGAGG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_018096659.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952148.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.

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CloneID	OXa36861
Clone ID Related Accession (Same CDS sequence)	XM_018096659.1 , XM_012969108.2
Accession Version	XM_018096659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCCAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAAC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952148.1
CDS	80..4498
Translation	MSTAPGLQTLWLSKVGSEGEEPQTLHSKETSTDGDLLTSESLRRHSKDDRQLLFLEETSHQGNDESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_018096659.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096659.1

ORF Insert Sequence:

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCCAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAAC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969108.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824562.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.

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CloneID	OXa36861
Clone ID Related Accession (Same CDS sequence)	XM_018096659.1 , XM_012969108.2
Accession Version	XM_012969108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCCAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAAC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824562.1
CDS	149..4567
Translation	MSTAPGLQTLWLSKVGSEGEEPQTLHSKETSTDGDLLTSESLRRHSKDDRQLLFLEETSHQGNDESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969108.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969108.2

ORF Insert Sequence:

ATGTCTACAG CTCCTGGTCT ACAGACTCTG TGGCTGAGCA AAGTTGGCAG TGAGGGTGAA 
GAGCCCCAGA CACTGCACTC TAAAGAGACT TCTACAGACG GAGACCTCTT AACCTCGGAG 
TCCTTGCGCA GGCATTCTAA AGATGATAGA CAGCTTCTGT TCCTTGAGGA AACATCGCAC 
CAGGGCAATG ATGAATCCCC ACGGCTGTCT GCCGCATCCT CAAGCAGCAA TGAAAAGCTC 
TCCATCTCAG CTTCAATCTG CAGTGATTCT GATAGGCCCT TGAGTCTCTT CTCAACAGAG 
TCCACAGGCT CTTTACGTGA AGGCTGTGAT TCCTATGAAG ATAACAGCGA CGTACCGCTG 
GGAAACGGAA CAGTGGTTCA CAACATGGAA CTGGTGGCCA CTGCAGCAGA GAGCCTGCAG 
GCCACCTACA GGGAGATGCA GAACGGTCTG AGGAACAACC ACACACCCAA CAATAATTCT 
GCCAATCGAC GCAGCCACCA GGCGTCTAGG TCCCTTTCTC CATTTGGTAA CAAGGTCAAT 
GCCATTACTC ACAAACTCAG CTATGTGGAA CGGGTGGTTC TGGAGATCAA TGACACAGAA 
AGAATGTATG TCCAGGATCT GCGCAGCATT GTGGAGGATT ACCTGGGAGC AATCATAGAC 
AGACAGGAAG AGCTGCCTAT AACCCCAGAG CAAGTCTGTG CTCTCTTTGG AAACATTGAG 
GACATTTATG AACTGAACAG TGAGCTGCTC CAGGACCTGG ATAATTGCAG GAATGACCCC 
GTGGCCGTGG CCAGCTGCTT TGTCGAAAAG AGTCAGGACT TTGACATCTA CACCCAGTAC 
TGCACCAACT ATCCCAACTC CGTGGCCACC TTGACGGAGT GCATGAGGAA TAAAACCCTG 
GCCAAGTTCT TTCGAGAGAA GCAGGAGATG CTGAACCAAT CCCTTCCACT CGGCTCCTAC 
CTCCTAAAGC CAGTGCAGAG AATACTGAAG TATCACCTCC TATTGCAGGA AATAGCAAAA 
CATCTTGATG TGAAGGTGGA AGGGTATGAG GTGCTGGAGG AGGCTATTGA AACCATGACG 
GGAGTAGCCT GGTACATTAA CGACATGAAA AGAAAGCACG AACATGCTGT GCGGCAGCAG 
GAGATCCAGT CTTTGCTGTT GAACTGGAAA GGTATAGATC TCACTGCATA TGGAGAACTT 
GTGCTGGAGG GAACATTTCG GCTCCAGCGA GCACGGAGTG AGAGGACATT TTTCCTTTTC 
GATAAGGCCC TGTTTATCAC CAAGAGACGT GGGGATCACT ATGTCTACAA AACTCATATC 
CCGTGTTCAA GCTTGATGCT GATTGAGAGT GCCCGTGACT CCCTCTGCTT TAGTGTGACC 
CATTACAAGA ACAGCAAACA GCAGCATAAT GTGCAGGCCA AAACAGTTGA AGAGAAACAT 
GTGTGGACCC ATCATATAAA GAAACTCATA CTAGAGAACC ACCATGCAAT CATTCCCCAA 
AAGGCGAAGG AGGCAATACT GGAAATGGAT AACATGTATA AAGGAAGGTA CAAATACAGC 
CCAGAGAGGC AAAAGAAGAC CATGTCTGCC ATTGAGCCCT CCATGACACA CCGGTCAGGA 
CGCAGACAGT CTGAGCCCTC CAAAGACATA CTAAAGCAAC TGAATGACAA AGCAGGATTA 
AAGCATGCAG GCAGTGATGG AGATCTTCAG GAATTTGGGG ACATTCCACA GCCTTGTATT 
GGCAGCTTGG GCACCAGCCT TGAGCAGCCA GAGGCTGAGG GAGACAAGGA GCGTCCTGTC 
AGCACACAGC TGAGTAGAAG CTCAGTGGCA GAGCTCAGTG CCTCTGATGA GGATGAGGAG 
GAGATACAGT TGGGAGATGA TGATAATGAT GATGATGATG AGCAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969111.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824565.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa36862
Clone ID Related Accession (Same CDS sequence)	XM_012969111.2
Accession Version	XM_012969111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGATG ATCGTGGGGG ACGCTTCGAA GGGCTTAATC GCAGGATCTC AAGGTTATTT 
GAATCCCCAC GGCTGTCTGC CGCATCCTCA AGCAGCAATG AAAAGCTCTC CATCTCAGCT 
TCAATCTGCA GTGATTCTGA TAGGCCCTTG AGTCTCTTCT CAACAGAGTC CACAGGCTCT 
TTACGTGAAG GCTGTGATTC CTATGAAGAT AACAGCGACG TACCGCTGGG AAACGGAACA 
GTGGTTCACA ACATGGAACT GGTGGCCACT GCAGCAGAGA GCCTGCAGGC CACCTACAGG 
GAGATGCAGA ACGGTCTGAG GAACAACCAC ACACCCAACA ATAATTCTGC CAATCGACGC 
AGCCACCAGG CGTCTAGGTC CCTTTCTCCA TTTGGTAACA AGGTCAATGC CATTACTCAC 
AAACTCAGCT ATGTGGAACG GGTGGTTCTG GAGATCAATG ACACAGAAAG AATGTATGTC 
CAGGATCTGC GCAGCATTGT GGAGGATTAC CTGGGAGCAA TCATAGACAG ACAGGAAGAG 
CTGCCTATAA CCCCAGAGCA AGTCTGTGCT CTCTTTGGAA ACATTGAGGA CATTTATGAA 
CTGAACAGTG AGCTGCTCCA GGACCTGGAT AATTGCAGGA ATGACCCCGT GGCCGTGGCC 
AGCTGCTTTG TCGAAAAGAG TCAGGACTTT GACATCTACA CCCAGTACTG CACCAACTAT 
CCCAACTCCG TGGCCACCTT GACGGAGTGC ATGAGGAATA AAACCCTGGC CAAGTTCTTT 
CGAGAGAAGC AGGAGATGCT GAACCAATCC CTTCCACTCG GCTCCTACCT CCTAAAGCCA 
GTGCAGAGAA TACTGAAGTA TCACCTCCTA TTGCAGGAAA TAGCAAAACA TCTTGATGTG 
AAGGTGGAAG GGTATGAGGT GCTGGAGGAG GCTATTGAAA CCATGACGGG AGTAGCCTGG 
TACATTAACG ACATGAAAAG AAAGCACGAA CATGCTGTGC GGCAGCAGGA GATCCAGTCT 
TTGCTGTTGA ACTGGAAAGG TATAGATCTC ACTGCATATG GAGAACTTGT GCTGGAGGGA 
ACATTTCGGC TCCAGCGAGC ACGGAGTGAG AGGACATTTT TCCTTTTCGA TAAGGCCCTG 
TTTATCACCA AGAGACGTGG GGATCACTAT GTCTACAAAA CTCATATCCC GTGTTCAAGC 
TTGATGCTGA TTGAGAGTGC CCGTGACTCC CTCTGCTTTA GTGTGACCCA TTACAAGAAC 
AGCAAACAGC AGCATAATGT GCAGGCCAAA ACAGTTGAAG AGAAACATGT GTGGACCCAT 
CATATAAAGA AACTCATACT AGAGAACCAC CATGCAATCA TTCCCCAAAA GGCGAAGGAG 
GCAATACTGG AAATGGATAA CATGTATAAA GGAAGGTACA AATACAGCCC AGAGAGGCAA 
AAGAAGACCA TGTCTGCCAT TGAGCCCTCC ATGACACACC GGTCAGGACG CAGACAGTCT 
GAGCCCTCCA AAGACATACT AAAGCAACTG AATGACAAAG CAGGATTAAA GCATGCAGGC 
AGTGATGGAG ATCTTCAGGA ATTTGGGGAC ATTCCACAGC CTTGTATTGG CAGCTTGGGC 
ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA GACAAGGAGC GTCCTGTCAG CACACAGCTG 
AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC TCTGATGAGG ATGAGGAGGA GATACAGTTG 
GGAGATGATG ATAATGATGA TGATGATGAG CAGACTAAAG ATCCGGTGGA GGAAGAGGCA 
CCAAAGGGCT GCAAGAGACA AAACAGCCAG TCATCTGGGA ATGCAGAGAA GAGGAGGAGT 
GTGGAGGTCA CATCTGGGCA CCAGGAGTCT GTATATAAAA TGGATAAGGA GAATTCAGAG 
CCAGAAGAGA TCTACAAGGC TGTGGTCGTC AGCTCTTCTG AGGATGAACA AGCTGTTCTG 
TTGGAAATCA CAGATGACAC AGAGCCAGCA AAAGATGATA GTGCAGCTCA CCCAGAAGTC 
CTTGAGCCTG TGGAGAATGA AGAAGCTGAA AGTACAGAAG ACTCAAAGAC TTTCAGCAGC 
GAAGAAGAGG AAGAGGAGAC ACCAACCCAT GAATCAACAA GTATCCTACC TCCTTCTGTT 
CTGGACCAGG CCAGTGTTAT TGCTGAGAGG TTTATAAGCA GCATGTCCAG GAGGAGTAGC 
ATGGCTCTAG ATGATGGGAA AGTCATAGGC TACATCACCC CCAGATTAAC CAGCCGCTGC 
AGTAGTTTGA TCAATCTGGA ATCTACAGAT ACTATTATTT ATCAGAACGG TTCAAGCGAC 
TTCCAGGACT TCTCAGAAGT TTGTGGTGAC ACTGGAGTAG ACATTCTTGA CACCACTTTT 
AGTAAGGTGA ACTCTGCCAG TATGGAGAAT GTGTTTGAGG AAAGAGAAAG GCCCACATGT 
AAGAGAAGAG ACTCTATTCT ATCCATGCAA GATAGACAAC TCATTGATAA AATCAGAACT 
TACTATGACG ATGCAGAAAA TCGGGATGCA TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG 
AACTATATTC CCACTGGACT TGTTAGAAAC TCTGTATTTA AGATTAACAG CCTGCCCAGA 
TTTGATACTA CCCAGAGTTC TCTATCGAGG CAGAGAATGT CCAGTTCATC TAGTAATACC 
AGCGGTGTGA ACAGTAGACA TGCTTCCTTG ACAGATACAA GGTCTAATGC AAGAGAATCA 
GAGCAGTTAG AGACGCAAAC TGAACTGCTT TTGGAAACAG CCACAAATGG CACTTCTGAC 
CATTCAGCCC CGATTACTGA TGAGGAGTTC CGGACACCAG ATGAAATGAT TAAAGTCTGG 
GATGAGTTTG AAAAGGTAAA TGCTTGGAAA GAGGCCTCAG ATATTTTAGA AGCCAAAAAA 
GATGTCAAAC CAGACTCTGT GGGTGAATGT ACAGCACACT GTGCCCTGGA GAATGGTTTG 
GAATCTCACG AACCACTGCT CATATTGGAA GACAGCGATC TGAGTACTAT AACAGAGGAA 
TCGCCTCTTC CTACACCGGA AAGCACATCT CCTAAACAGT TTGGCTGTGT TCCCAATAAC 
ACAAGTTTTA GTGAGTACAA AACGCCTGTG AAGCTACACT CCAAGATAAT GCAAATGGCC 
AGCTGCATGG ATGAAGACAT GTCGGAGAAG ATGAAGAACA AGGTGTACCA GCTGGCCAGG 
CAGTACAGTC AGAGGATCAA ATGCAACAAG CCAGTGTCCC AAAGGCAGCT AAAGGAGATA 
GAGGAGGATC TCAGAAGGAA CAGCCTGCCT GCTGTGCAAG AGGAAAAACT GGATAAGAAA 
GAGAAACGCA AGCCAATACT GTCACTCCCC ACATACGACA GCGTTATACT CCAGGAGCAT 
GGCCCTACAA CCCCAACATC GGCCCTGAGC AGAGACACAG CTCCAAAGCA TCTTTCCTTT 
TCACCATCCT GTGACAGCCC ACGGGCTCTT TCCCCAGAGA GAGCTGACTG CAGGAGCCCT 
CTGAGTCCAG TCAAGGCAGA GAGATTTCAC TGGCCTGACG TCCGAGAACT GAGATCTAGG 
TACACAAGTG CAGCCAGCTC TAGCAAGGCT CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA 
GAAAGAATAA CTGTGCCCAA TTTGAGGGGG CATTCTGACA CACAAAGTAA AGGTTGCTTG 
ATTCACTCTC ACAGCATGAA CGTGAAACAC ACAGAAAATG TCAATGGCAG CGCTGAGGAC 
TCGGCACAGA ATAACAAAGC ACAGGTAGAA ACTGAGCCGC CTTTGCCAGT GTCACCCAGT 
CTAAAAGGTA AAACTGATTC ACTACTTGGC TCTGCAAAGC CTGGCTATTA CATCAGTGCA 
GAGGCTCCTT TGGAAAATAA CAAGAAAGTT ATCGTTATGG AAAAAATTCC TGCTGCCACA 
GACTCTAATG GCTCCCATGA TGTGGAAAGC GATGACACTT ACGTACAAAT CCGATCCCCC 
ACAAGCAGAG AGAAGATCTC TCTCAAAGCC GTGATTGAGC GCTGCAAAGC CTACCAGGAG 
TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC TCCAAGGATG GGGAAACCAC TGAATGCACT 
GAATCTGTTC CCCATGAAGA GCCCAATCTG GAGAGGCACG TGGCACCAAG CTGCGATGTC 
AACCAGCAGA ACCGAGTCAA GAACTTGAGA GAAAAGTTTC AGACTAAGCA CAACTCAAGT 
AGTTTGAAAC AATCGGAGGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824565.1
CDS	182..4468
Translation	MFDDRGGRFEGLNRRISRLFESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969111.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969111.2

ORF Insert Sequence:

ATGTTTGATG ATCGTGGGGG ACGCTTCGAA GGGCTTAATC GCAGGATCTC AAGGTTATTT 
GAATCCCCAC GGCTGTCTGC CGCATCCTCA AGCAGCAATG AAAAGCTCTC CATCTCAGCT 
TCAATCTGCA GTGATTCTGA TAGGCCCTTG AGTCTCTTCT CAACAGAGTC CACAGGCTCT 
TTACGTGAAG GCTGTGATTC CTATGAAGAT AACAGCGACG TACCGCTGGG AAACGGAACA 
GTGGTTCACA ACATGGAACT GGTGGCCACT GCAGCAGAGA GCCTGCAGGC CACCTACAGG 
GAGATGCAGA ACGGTCTGAG GAACAACCAC ACACCCAACA ATAATTCTGC CAATCGACGC 
AGCCACCAGG CGTCTAGGTC CCTTTCTCCA TTTGGTAACA AGGTCAATGC CATTACTCAC 
AAACTCAGCT ATGTGGAACG GGTGGTTCTG GAGATCAATG ACACAGAAAG AATGTATGTC 
CAGGATCTGC GCAGCATTGT GGAGGATTAC CTGGGAGCAA TCATAGACAG ACAGGAAGAG 
CTGCCTATAA CCCCAGAGCA AGTCTGTGCT CTCTTTGGAA ACATTGAGGA CATTTATGAA 
CTGAACAGTG AGCTGCTCCA GGACCTGGAT AATTGCAGGA ATGACCCCGT GGCCGTGGCC 
AGCTGCTTTG TCGAAAAGAG TCAGGACTTT GACATCTACA CCCAGTACTG CACCAACTAT 
CCCAACTCCG TGGCCACCTT GACGGAGTGC ATGAGGAATA AAACCCTGGC CAAGTTCTTT 
CGAGAGAAGC AGGAGATGCT GAACCAATCC CTTCCACTCG GCTCCTACCT CCTAAAGCCA 
GTGCAGAGAA TACTGAAGTA TCACCTCCTA TTGCAGGAAA TAGCAAAACA TCTTGATGTG 
AAGGTGGAAG GGTATGAGGT GCTGGAGGAG GCTATTGAAA CCATGACGGG AGTAGCCTGG 
TACATTAACG ACATGAAAAG AAAGCACGAA CATGCTGTGC GGCAGCAGGA GATCCAGTCT 
TTGCTGTTGA ACTGGAAAGG TATAGATCTC ACTGCATATG GAGAACTTGT GCTGGAGGGA 
ACATTTCGGC TCCAGCGAGC ACGGAGTGAG AGGACATTTT TCCTTTTCGA TAAGGCCCTG 
TTTATCACCA AGAGACGTGG GGATCACTAT GTCTACAAAA CTCATATCCC GTGTTCAAGC 
TTGATGCTGA TTGAGAGTGC CCGTGACTCC CTCTGCTTTA GTGTGACCCA TTACAAGAAC 
AGCAAACAGC AGCATAATGT GCAGGCCAAA ACAGTTGAAG AGAAACATGT GTGGACCCAT 
CATATAAAGA AACTCATACT AGAGAACCAC CATGCAATCA TTCCCCAAAA GGCGAAGGAG 
GCAATACTGG AAATGGATAA CATGTATAAA GGAAGGTACA AATACAGCCC AGAGAGGCAA 
AAGAAGACCA TGTCTGCCAT TGAGCCCTCC ATGACACACC GGTCAGGACG CAGACAGTCT 
GAGCCCTCCA AAGACATACT AAAGCAACTG AATGACAAAG CAGGATTAAA GCATGCAGGC 
AGTGATGGAG ATCTTCAGGA ATTTGGGGAC ATTCCACAGC CTTGTATTGG CAGCTTGGGC 
ACCAGCCTTG AGCAGCCAGA GGCTGAGGGA GACAAGGAGC GTCCTGTCAG CACACAGCTG 
AGTAGAAGCT CAGTGGCAGA GCTCAGTGCC TCTGATGAGG ATGAGGAGGA GATACAGTTG 
GGAGATGATG ATAATGATGA TGATGATGAG CAGACTAAAG ATCCGGTGGA GGAAGAGGCA 
CCAAAGGGCT GCAAGAGACA AAACAGCCAG TCATCTGGGA ATGCAGAGAA GAGGAGGAGT 
GTGGAGGTCA CATCTGGGCA CCAGGAGTCT GTATATAAAA TGGATAAGGA GAATTCAGAG 
CCAGAAGAGA TCTACAAGGC TGTGGTCGTC AGCTCTTCTG AGGATGAACA AGCTGTTCTG 
TTGGAAATCA CAGATGACAC AGAGCCAGCA AAAGATGATA GTGCAGCTCA CCCAGAAGTC 
CTTGAGCCTG TGGAGAATGA AGAAGCTGAA AGTACAGAAG ACTCAAAGAC TTTCAGCAGC 
GAAGAAGAGG AAGAGGAGAC ACCAACCCAT GAATCAACAA GTATCCTACC TCCTTCTGTT 
CTGGACCAGG CCAGTGTTAT TGCTGAGAGG TTTATAAGCA GCATGTCCAG GAGGAGTAGC 
ATGGCTCTAG ATGATGGGAA AGTCATAGGC TACATCACCC CCAGATTAAC CAGCCGCTGC 
AGTAGTTTGA TCAATCTGGA ATCTACAGAT ACTATTATTT ATCAGAACGG TTCAAGCGAC 
TTCCAGGACT TCTCAGAAGT TTGTGGTGAC ACTGGAGTAG ACATTCTTGA CACCACTTTT 
AGTAAGGTGA ACTCTGCCAG TATGGAGAAT GTGTTTGAGG AAAGAGAAAG GCCCACATGT 
AAGAGAAGAG ACTCTATTCT ATCCATGCAA GATAGACAAC TCATTGATAA AATCAGAACT 
TACTATGACG ATGCAGAAAA TCGGGATGCA TCCTTTAGCA TTAAGCGCAG AGAGAGCTTG 
AACTATATTC CCACTGGACT TGTTAGAAAC TCTGTATTTA AGATTAACAG CCTGCCCAGA 
TTTGATACTA CCCAGAGTTC TCTATCGAGG CAGAGAATGT CCAGTTCATC TAGTAATACC 
AGCGGTGTGA ACAGTAGACA TGCTTCCTTG ACAGATACAA GGTCTAATGC AAGAGAATCA 
GAGCAGTTAG AGACGCAAAC TGAACTGCTT TTGGAAACAG CCACAAATGG CACTTCTGAC 
CATTCAGCCC CGATTACTGA TGAGGAGTTC CGGACACCAG ATGAAATGAT TAAAGTCTGG 
GATGAGTTTG AAAAGGTAAA TGCTTGGAAA GAGGCCTCAG ATATTTTAGA AGCCAAAAAA 
GATGTCAAAC CAGACTCTGT GGGTGAATGT ACAGCACACT GTGCCCTGGA GAATGGTTTG 
GAATCTCACG AACCACTGCT CATATTGGAA GACAGCGATC TGAGTACTAT AACAGAGGAA 
TCGCCTCTTC CTACACCGGA AAGCACATCT CCTAAACAGT TTGGCTGTGT TCCCAATAAC 
ACAAGTTTTA GTGAGTACAA AACGCCTGTG AAGCTACACT CCAAGATAAT GCAAATGGCC 
AGCTGCATGG ATGAAGACAT GTCGGAGAAG ATGAAGAACA AGGTGTACCA GCTGGCCAGG 
CAGTACAGTC AGAGGATCAA ATGCAACAAG CCAGTGTCCC AAAGGCAGCT AAAGGAGATA 
GAGGAGGATC TCAGAAGGAA CAGCCTGCCT GCTGTGCAAG AGGAAAAACT GGATAAGAAA 
GAGAAACGCA AGCCAATACT GTCACTCCCC ACATACGACA GCGTTATACT CCAGGAGCAT 
GGCCCTACAA CCCCAACATC GGCCCTGAGC AGAGACACAG CTCCAAAGCA TCTTTCCTTT 
TCACCATCCT GTGACAGCCC ACGGGCTCTT TCCCCAGAGA GAGCTGACTG CAGGAGCCCT 
CTGAGTCCAG TCAAGGCAGA GAGATTTCAC TGGCCTGACG TCCGAGAACT GAGATCTAGG 
TACACAAGTG CAGCCAGCTC TAGCAAGGCT CCTCCCGTCA ACAGGAGCCA GTCTGTGCCA 
GAAAGAATAA CTGTGCCCAA TTTGAGGGGG CATTCTGACA CACAAAGTAA AGGTTGCTTG 
ATTCACTCTC ACAGCATGAA CGTGAAACAC ACAGAAAATG TCAATGGCAG CGCTGAGGAC 
TCGGCACAGA ATAACAAAGC ACAGGTAGAA ACTGAGCCGC CTTTGCCAGT GTCACCCAGT 
CTAAAAGGTA AAACTGATTC ACTACTTGGC TCTGCAAAGC CTGGCTATTA CATCAGTGCA 
GAGGCTCCTT TGGAAAATAA CAAGAAAGTT ATCGTTATGG AAAAAATTCC TGCTGCCACA 
GACTCTAATG GCTCCCATGA TGTGGAAAGC GATGACACTT ACGTACAAAT CCGATCCCCC 
ACAAGCAGAG AGAAGATCTC TCTCAAAGCC GTGATTGAGC GCTGCAAAGC CTACCAGGAG 
TCCGAGGAGT ACCGGTCCAG GGAAGTAGCC TCCAAGGATG GGGAAACCAC TGAATGCACT 
GAATCTGTTC CCCATGAAGA GCCCAATCTG GAGAGGCACG TGGCACCAAG CTGCGATGTC 
AACCAGCAGA ACCGAGTCAA GAACTTGAGA GAAAAGTTTC AGACTAAGCA CAACTCAAGT 
AGTTTGAAAC AATCGGAGGT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917285.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917342.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.

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CloneID	OXa36863
Clone ID Related Accession (Same CDS sequence)	XM_004917285.3
Accession Version	XM_004917285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGCAGGAT TAAAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917342.2
CDS	55..4233
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917285.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917285.3

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGCAGGAT TAAAGACTAA AGATCCGGTG 
GAGGAAGAGG CACCAAAGGG CTGCAAGAGA CAAAACAGCC AGTCATCTGG GAATGCAGAG 
AAGAGGAGGA GTGTGGAGGT CACATCTGGG CACCAGGAGT CTGTATATAA AATGGATAAG 
GAGAATTCAG AGCCAGAAGA GATCTACAAG GCTGTGGTCG TCAGCTCTTC TGAGGATGAA 
CAAGCTGTTC TGTTGGAAAT CACAGATGAC ACAGAGCCAG CAAAAGATGA TAGTGCAGCT 
CACCCAGAAG TCCTTGAGCC TGTGGAGAAT GAAGAAGCTG AAAGTACAGA AGACTCAAAG 
ACTTTCAGCA GCGAAGAAGA GGAAGAGGAG ACACCAACCC ATGAATCAAC AAGTATCCTA 
CCTCCTTCTG TTCTGGACCA GGCCAGTGTT ATTGCTGAGA GGTTTATAAG CAGCATGTCC 
AGGAGGAGTA GCATGGCTCT AGATGATGGG AAAGTCATAG GCTACATCAC CCCCAGATTA 
ACCAGCCGCT GCAGTAGTTT GATCAATCTG GAATCTACAG ATACTATTAT TTATCAGAAC 
GGTTCAAGCG ACTTCCAGGA CTTCTCAGAA GTTTGTGGTG ACACTGGAGT AGACATTCTT 
GACACCACTT TTAGTAAGGT GAACTCTGCC AGTATGGAGA ATGTGTTTGA GGAAAGAGAA 
AGGCCCACAT GTAAGAGAAG AGACTCTATT CTATCCATGC AAGATAGACA ACTCATTGAT 
AAAATCAGAA CTTACTATGA CGATGCAGAA AATCGGGATG CATCCTTTAG CATTAAGCGC 
AGAGAGAGCT TGAACTATAT TCCCACTGGA CTTGTTAGAA ACTCTGTATT TAAGATTAAC 
AGCCTGCCCA GATTTGATAC TACCCAGAGT TCTCTATCGA GGCAGAGAAT GTCCAGTTCA 
TCTAGTAATA CCAGCGGTGT GAACAGTAGA CATGCTTCCT TGACAGATAC AAGGTCTAAT 
GCAAGAGAAT CAGAGCAGTT AGAGACGCAA ACTGAACTGC TTTTGGAAAC AGCCACAAAT 
GGCACTTCTG ACCATTCAGC CCCGATTACT GATGAGGAGT TCCGGACACC AGATGAAATG 
ATTAAAGTCT GGGATGAGTT TGAAAAGGTA AATGCTTGGA AAGAGGCCTC AGATATTTTA 
GAAGCCAAAA AAGATGTCAA ACCAGACTCT GTGGGTGAAT GTACAGCACA CTGTGCCCTG 
GAGAATGGTT TGGAATCTCA CGAACCACTG CTCATATTGG AAGACAGCGA TCTGAGTACT 
ATAACAGAGG AATCGCCTCT TCCTACACCG GAAAGCACAT CTCCTAAACA GTTTGGCTGT 
GTTCCCAATA ACACAAGTTT TAGTGAGTAC AAAACGCCTG TGAAGCTACA CTCCAAGATA 
ATGCAAATGG CCAGCTGCAT GGATGAAGAC ATGTCGGAGA AGATGAAGAA CAAGGTGTAC 
CAGCTGGCCA GGCAGTACAG TCAGAGGATC AAATGCAACA AGCCAGTGTC CCAAAGGCAG 
CTAAAGGAGA TAGAGGAGGA TCTCAGAAGG AACAGCCTGC CTGCTGTGCA AGAGGAAAAA 
CTGGATAAGA AAGAGAAACG CAAGCCAATA CTGTCACTCC CCACATACGA CAGCGTTATA 
CTCCAGGAGC ATGGCCCTAC AACCCCAACA TCGGCCCTGA GCAGAGACAC AGCTCCAAAG 
CATCTTTCCT TTTCACCATC CTGTGACAGC CCACGGGCTC TTTCCCCAGA GAGAGCTGAC 
TGCAGGAGCC CTCTGAGTCC AGTCAAGGCA GAGAGATTTC ACTGGCCTGA CGTCCGAGAA 
CTGAGATCTA GGTACACAAG TGCAGCCAGC TCTAGCAAGG CTCCTCCCGT CAACAGGAGC 
CAGTCTGTGC CAGAAAGAAT AACTGTGCCC AATTTGAGGG GGCATTCTGA CACACAAAGT 
AAAGGTTGCT TGATTCACTC TCACAGCATG AACGTGAAAC ACACAGAAAA TGTCAATGGC 
AGCGCTGAGG ACTCGGCACA GAATAACAAA GCACAGGTAG AAACTGAGCC GCCTTTGCCA 
GTGTCACCCA GTCTAAAAGG TAAAACTGAT TCACTACTTG GCTCTGCAAA GCCTGGCTAT 
TACATCAGTG CAGAGGCTCC TTTGGAAAAT AACAAGAAAG TTATCGTTAT GGAAAAAATT 
CCTGCTGCCA CAGACTCTAA TGGCTCCCAT GATGTGGAAA GCGATGACAC TTACGTACAA 
ATCCGATCCC CCACAAGCAG AGAGAAGATC TCTCTCAAAG CCGTGATTGA GCGCTGCAAA 
GCCTACCAGG AGTCCGAGGA GTACCGGTCC AGGGAAGTAG CCTCCAAGGA TGGGGAAACC 
ACTGAATGCA CTGAATCTGT TCCCCATGAA GAGCCCAATC TGGAGAGGCA CGTGGCACCA 
AGCTGCGATG TCAACCAGCA GAACCGAGTC AAGAACTTGA GAGAAAAGTT TCAGACTAAG 
CACAACTCAA GTAGTTTGAA ACAATCGGAG GTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_004917286.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917343.2 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.

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CloneID	OXa36864
Clone ID Related Accession (Same CDS sequence)	XM_004917286.3
Accession Version	XM_004917286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGGATTAA AGACTAAAGA TCCGGTGGAG 
GAAGAGGCAC CAAAGGGCTG CAAGAGACAA AACAGCCAGT CATCTGGGAA TGCAGAGAAG 
AGGAGGAGTG TGGAGGTCAC ATCTGGGCAC CAGGAGTCTG TATATAAAAT GGATAAGGAG 
AATTCAGAGC CAGAAGAGAT CTACAAGGCT GTGGTCGTCA GCTCTTCTGA GGATGAACAA 
GCTGTTCTGT TGGAAATCAC AGATGACACA GAGCCAGCAA AAGATGATAG TGCAGCTCAC 
CCAGAAGTCC TTGAGCCTGT GGAGAATGAA GAAGCTGAAA GTACAGAAGA CTCAAAGACT 
TTCAGCAGCG AAGAAGAGGA AGAGGAGACA CCAACCCATG AATCAACAAG TATCCTACCT 
CCTTCTGTTC TGGACCAGGC CAGTGTTATT GCTGAGAGGT TTATAAGCAG CATGTCCAGG 
AGGAGTAGCA TGGCTCTAGA TGATGGGAAA GTCATAGGCT ACATCACCCC CAGATTAACC 
AGCCGCTGCA GTAGTTTGAT CAATCTGGAA TCTACAGATA CTATTATTTA TCAGAACGGT 
TCAAGCGACT TCCAGGACTT CTCAGAAGTT TGTGGTGACA CTGGAGTAGA CATTCTTGAC 
ACCACTTTTA GTAAGGTGAA CTCTGCCAGT ATGGAGAATG TGTTTGAGGA AAGAGAAAGG 
CCCACATGTA AGAGAAGAGA CTCTATTCTA TCCATGCAAG ATAGACAACT CATTGATAAA 
ATCAGAACTT ACTATGACGA TGCAGAAAAT CGGGATGCAT CCTTTAGCAT TAAGCGCAGA 
GAGAGCTTGA ACTATATTCC CACTGGACTT GTTAGAAACT CTGTATTTAA GATTAACAGC 
CTGCCCAGAT TTGATACTAC CCAGAGTTCT CTATCGAGGC AGAGAATGTC CAGTTCATCT 
AGTAATACCA GCGGTGTGAA CAGTAGACAT GCTTCCTTGA CAGATACAAG GTCTAATGCA 
AGAGAATCAG AGCAGTTAGA GACGCAAACT GAACTGCTTT TGGAAACAGC CACAAATGGC 
ACTTCTGACC ATTCAGCCCC GATTACTGAT GAGGAGTTCC GGACACCAGA TGAAATGATT 
AAAGTCTGGG ATGAGTTTGA AAAGGTAAAT GCTTGGAAAG AGGCCTCAGA TATTTTAGAA 
GCCAAAAAAG ATGTCAAACC AGACTCTGTG GGTGAATGTA CAGCACACTG TGCCCTGGAG 
AATGGTTTGG AATCTCACGA ACCACTGCTC ATATTGGAAG ACAGCGATCT GAGTACTATA 
ACAGAGGAAT CGCCTCTTCC TACACCGGAA AGCACATCTC CTAAACAGTT TGGCTGTGTT 
CCCAATAACA CAAGTTTTAG TGAGTACAAA ACGCCTGTGA AGCTACACTC CAAGATAATG 
CAAATGGCCA GCTGCATGGA TGAAGACATG TCGGAGAAGA TGAAGAACAA GGTGTACCAG 
CTGGCCAGGC AGTACAGTCA GAGGATCAAA TGCAACAAGC CAGTGTCCCA AAGGCAGCTA 
AAGGAGATAG AGGAGGATCT CAGAAGGAAC AGCCTGCCTG CTGTGCAAGA GGAAAAACTG 
GATAAGAAAG AGAAACGCAA GCCAATACTG TCACTCCCCA CATACGACAG CGTTATACTC 
CAGGAGCATG GCCCTACAAC CCCAACATCG GCCCTGAGCA GAGACACAGC TCCAAAGCAT 
CTTTCCTTTT CACCATCCTG TGACAGCCCA CGGGCTCTTT CCCCAGAGAG AGCTGACTGC 
AGGAGCCCTC TGAGTCCAGT CAAGGCAGAG AGATTTCACT GGCCTGACGT CCGAGAACTG 
AGATCTAGGT ACACAAGTGC AGCCAGCTCT AGCAAGGCTC CTCCCGTCAA CAGGAGCCAG 
TCTGTGCCAG AAAGAATAAC TGTGCCCAAT TTGAGGGGGC ATTCTGACAC ACAAAGTAAA 
GGTTGCTTGA TTCACTCTCA CAGCATGAAC GTGAAACACA CAGAAAATGT CAATGGCAGC 
GCTGAGGACT CGGCACAGAA TAACAAAGCA CAGGTAGAAA CTGAGCCGCC TTTGCCAGTG 
TCACCCAGTC TAAAAGGTAA AACTGATTCA CTACTTGGCT CTGCAAAGCC TGGCTATTAC 
ATCAGTGCAG AGGCTCCTTT GGAAAATAAC AAGAAAGTTA TCGTTATGGA AAAAATTCCT 
GCTGCCACAG ACTCTAATGG CTCCCATGAT GTGGAAAGCG ATGACACTTA CGTACAAATC 
CGATCCCCCA CAAGCAGAGA GAAGATCTCT CTCAAAGCCG TGATTGAGCG CTGCAAAGCC 
TACCAGGAGT CCGAGGAGTA CCGGTCCAGG GAAGTAGCCT CCAAGGATGG GGAAACCACT 
GAATGCACTG AATCTGTTCC CCATGAAGAG CCCAATCTGG AGAGGCACGT GGCACCAAGC 
TGCGATGTCA ACCAGCAGAA CCGAGTCAAG AACTTGAGAG AAAAGTTTCA GACTAAGCAC 
AACTCAAGTA GTTTGAAACA ATCGGAGGTT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917343.2
CDS	56..4231
Translation	MRAVRSPQFRTGTISVKGELGGVRRRRTPPGCGSRLQRAGGTEYGCGLGSGMRRSGRSESPRLSAASSSSNEKLSISASICSDSDRPLSLFSTESTGSLREGCDSYEDNSDVPLGNGTVVHNMELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKGLKTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_004917286.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917286.3

ORF Insert Sequence:

ATGCGAGCAG TCCGGTCCCC GCAGTTTAGA ACTGGGACCA TAAGCGTCAA AGGGGAGCTG 
GGCGGTGTCA GACGGAGGAG AACGCCCCCT GGGTGTGGCT CTCGGCTGCA GCGGGCTGGC 
GGCACAGAGT ACGGGTGCGG TTTAGGTAGC GGGATGCGGA GATCCGGCCG GTCCGAATCC 
CCACGGCTGT CTGCCGCATC CTCAAGCAGC AATGAAAAGC TCTCCATCTC AGCTTCAATC 
TGCAGTGATT CTGATAGGCC CTTGAGTCTC TTCTCAACAG AGTCCACAGG CTCTTTACGT 
GAAGGCTGTG ATTCCTATGA AGATAACAGC GACGTACCGC TGGGAAACGG AACAGTGGTT 
CACAACATGG AACTGGTGGC CACTGCAGCA GAGAGCCTGC AGGCCACCTA CAGGGAGATG 
CAGAACGGTC TGAGGAACAA CCACACACCC AACAATAATT CTGCCAATCG ACGCAGCCAC 
CAGGCGTCTA GGTCCCTTTC TCCATTTGGT AACAAGGTCA ATGCCATTAC TCACAAACTC 
AGCTATGTGG AACGGGTGGT TCTGGAGATC AATGACACAG AAAGAATGTA TGTCCAGGAT 
CTGCGCAGCA TTGTGGAGGA TTACCTGGGA GCAATCATAG ACAGACAGGA AGAGCTGCCT 
ATAACCCCAG AGCAAGTCTG TGCTCTCTTT GGAAACATTG AGGACATTTA TGAACTGAAC 
AGTGAGCTGC TCCAGGACCT GGATAATTGC AGGAATGACC CCGTGGCCGT GGCCAGCTGC 
TTTGTCGAAA AGAGTCAGGA CTTTGACATC TACACCCAGT ACTGCACCAA CTATCCCAAC 
TCCGTGGCCA CCTTGACGGA GTGCATGAGG AATAAAACCC TGGCCAAGTT CTTTCGAGAG 
AAGCAGGAGA TGCTGAACCA ATCCCTTCCA CTCGGCTCCT ACCTCCTAAA GCCAGTGCAG 
AGAATACTGA AGTATCACCT CCTATTGCAG GAAATAGCAA AACATCTTGA TGTGAAGGTG 
GAAGGGTATG AGGTGCTGGA GGAGGCTATT GAAACCATGA CGGGAGTAGC CTGGTACATT 
AACGACATGA AAAGAAAGCA CGAACATGCT GTGCGGCAGC AGGAGATCCA GTCTTTGCTG 
TTGAACTGGA AAGGTATAGA TCTCACTGCA TATGGAGAAC TTGTGCTGGA GGGAACATTT 
CGGCTCCAGC GAGCACGGAG TGAGAGGACA TTTTTCCTTT TCGATAAGGC CCTGTTTATC 
ACCAAGAGAC GTGGGGATCA CTATGTCTAC AAAACTCATA TCCCGTGTTC AAGCTTGATG 
CTGATTGAGA GTGCCCGTGA CTCCCTCTGC TTTAGTGTGA CCCATTACAA GAACAGCAAA 
CAGCAGCATA ATGTGCAGGC CAAAACAGTT GAAGAGAAAC ATGTGTGGAC CCATCATATA 
AAGAAACTCA TACTAGAGAA CCACCATGCA ATCATTCCCC AAAAGGCGAA GGAGGCAATA 
CTGGAAATGG ATAACATGTA TAAAGGAAGG TACAAATACA GCCCAGAGAG GCAAAAGAAG 
ACCATGTCTG CCATTGAGCC CTCCATGACA CACCGGTCAG GACGCAGACA GTCTGAGCCC 
TCCAAAGACA TACTAAAGCA ACTGAATGAC AAAGGATTAA AGACTAAAGA TCCGGTGGAG 
GAAGAGGCAC CAAAGGGCTG CAAGAGACAA AACAGCCAGT CATCTGGGAA TGCAGAGAAG 
AGGAGGAGTG TGGAGGTCAC ATCTGGGCAC CAGGAGTCTG TATATAAAAT GGATAAGGAG 
AATTCAGAGC CAGAAGAGAT CTACAAGGCT GTGGTCGTCA GCTCTTCTGA GGATGAACAA 
GCTGTTCTGT TGGAAATCAC AGATGACACA GAGCCAGCAA AAGATGATAG TGCAGCTCAC 
CCAGAAGTCC TTGAGCCTGT GGAGAATGAA GAAGCTGAAA GTACAGAAGA CTCAAAGACT 
TTCAGCAGCG AAGAAGAGGA AGAGGAGACA CCAACCCATG AATCAACAAG TATCCTACCT 
CCTTCTGTTC TGGACCAGGC CAGTGTTATT GCTGAGAGGT TTATAAGCAG CATGTCCAGG 
AGGAGTAGCA TGGCTCTAGA TGATGGGAAA GTCATAGGCT ACATCACCCC CAGATTAACC 
AGCCGCTGCA GTAGTTTGAT CAATCTGGAA TCTACAGATA CTATTATTTA TCAGAACGGT 
TCAAGCGACT TCCAGGACTT CTCAGAAGTT TGTGGTGACA CTGGAGTAGA CATTCTTGAC 
ACCACTTTTA GTAAGGTGAA CTCTGCCAGT ATGGAGAATG TGTTTGAGGA AAGAGAAAGG 
CCCACATGTA AGAGAAGAGA CTCTATTCTA TCCATGCAAG ATAGACAACT CATTGATAAA 
ATCAGAACTT ACTATGACGA TGCAGAAAAT CGGGATGCAT CCTTTAGCAT TAAGCGCAGA 
GAGAGCTTGA ACTATATTCC CACTGGACTT GTTAGAAACT CTGTATTTAA GATTAACAGC 
CTGCCCAGAT TTGATACTAC CCAGAGTTCT CTATCGAGGC AGAGAATGTC CAGTTCATCT 
AGTAATACCA GCGGTGTGAA CAGTAGACAT GCTTCCTTGA CAGATACAAG GTCTAATGCA 
AGAGAATCAG AGCAGTTAGA GACGCAAACT GAACTGCTTT TGGAAACAGC CACAAATGGC 
ACTTCTGACC ATTCAGCCCC GATTACTGAT GAGGAGTTCC GGACACCAGA TGAAATGATT 
AAAGTCTGGG ATGAGTTTGA AAAGGTAAAT GCTTGGAAAG AGGCCTCAGA TATTTTAGAA 
GCCAAAAAAG ATGTCAAACC AGACTCTGTG GGTGAATGTA CAGCACACTG TGCCCTGGAG 
AATGGTTTGG AATCTCACGA ACCACTGCTC ATATTGGAAG ACAGCGATCT GAGTACTATA 
ACAGAGGAAT CGCCTCTTCC TACACCGGAA AGCACATCTC CTAAACAGTT TGGCTGTGTT 
CCCAATAACA CAAGTTTTAG TGAGTACAAA ACGCCTGTGA AGCTACACTC CAAGATAATG 
CAAATGGCCA GCTGCATGGA TGAAGACATG TCGGAGAAGA TGAAGAACAA GGTGTACCAG 
CTGGCCAGGC AGTACAGTCA GAGGATCAAA TGCAACAAGC CAGTGTCCCA AAGGCAGCTA 
AAGGAGATAG AGGAGGATCT CAGAAGGAAC AGCCTGCCTG CTGTGCAAGA GGAAAAACTG 
GATAAGAAAG AGAAACGCAA GCCAATACTG TCACTCCCCA CATACGACAG CGTTATACTC 
CAGGAGCATG GCCCTACAAC CCCAACATCG GCCCTGAGCA GAGACACAGC TCCAAAGCAT 
CTTTCCTTTT CACCATCCTG TGACAGCCCA CGGGCTCTTT CCCCAGAGAG AGCTGACTGC 
AGGAGCCCTC TGAGTCCAGT CAAGGCAGAG AGATTTCACT GGCCTGACGT CCGAGAACTG 
AGATCTAGGT ACACAAGTGC AGCCAGCTCT AGCAAGGCTC CTCCCGTCAA CAGGAGCCAG 
TCTGTGCCAG AAAGAATAAC TGTGCCCAAT TTGAGGGGGC ATTCTGACAC ACAAAGTAAA 
GGTTGCTTGA TTCACTCTCA CAGCATGAAC GTGAAACACA CAGAAAATGT CAATGGCAGC 
GCTGAGGACT CGGCACAGAA TAACAAAGCA CAGGTAGAAA CTGAGCCGCC TTTGCCAGTG 
TCACCCAGTC TAAAAGGTAA AACTGATTCA CTACTTGGCT CTGCAAAGCC TGGCTATTAC 
ATCAGTGCAG AGGCTCCTTT GGAAAATAAC AAGAAAGTTA TCGTTATGGA AAAAATTCCT 
GCTGCCACAG ACTCTAATGG CTCCCATGAT GTGGAAAGCG ATGACACTTA CGTACAAATC 
CGATCCCCCA CAAGCAGAGA GAAGATCTCT CTCAAAGCCG TGATTGAGCG CTGCAAAGCC 
TACCAGGAGT CCGAGGAGTA CCGGTCCAGG GAAGTAGCCT CCAAGGATGG GGAAACCACT 
GAATGCACTG AATCTGTTCC CCATGAAGAG CCCAATCTGG AGAGGCACGT GGCACCAAGC 
TGCGATGTCA ACCAGCAGAA CCGAGTCAAG AACTTGAGAG AAAAGTTTCA GACTAAGCAC 
AACTCAAGTA GTTTGAAACA ATCGGAGGTT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_012969112.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824566.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa36865
Clone ID Related Accession (Same CDS sequence)	XM_012969112.2 , XM_018096660.1
Accession Version	XM_012969112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCCAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAACTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAATGTAC AGCACACTGT GCCCTGGAGA ATGGTTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATCTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC ACCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAAG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAGGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824566.1
CDS	424..4458
Translation	MELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_012969112.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969112.2

ORF Insert Sequence:

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCCAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAACTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAATGTAC AGCACACTGT GCCCTGGAGA ATGGTTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATCTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC ACCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAAG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAGGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plekhg3 ( XM_018096660.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952149.1 Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X9, mRNA.

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CloneID	OXa36865
Clone ID Related Accession (Same CDS sequence)	XM_012969112.2 , XM_018096660.1
Accession Version	XM_018096660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pleckstrin homology domain-containing family G member 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCCAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAACTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAATGTAC AGCACACTGT GCCCTGGAGA ATGGTTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATCTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC ACCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAAG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAGGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952149.1
CDS	310..4344
Translation	MELVATAAESLQATYREMQNGLRNNHTPNNNSANRRSHQASRSLSPFGNKVNAITHKLSYVERVVLEINDTERMYVQDLRSIVEDYLGAIIDRQEELPITPEQVCALFGNIEDIYELNSELLQDLDNCRNDPVAVASCFVEKSQDFDIYTQYCTNYPNSVATLTECMRNKTLAKFFREKQEMLNQSLPLGSYLLKPVQRILKYHLLLQEIAKHLDVKVEGYEVLEEAIETMTGVAWYINDMKRKHEHAVRQQEIQSLLLNWKGIDLTAYGELVLEGTFRLQRARSERTFFLFDKALFITKRRGDHYVYKTHIPCSSLMLIESARDSLCFSVTHYKNSKQQHNVQAKTVEEKHVWTHHIKKLILENHHAIIPQKAKEAILEMDNMYKGRYKYSPERQKKTMSAIEPSMTHRSGRRQSEPSKDILKQLNDKAGLKHAGSDGDLQEFGDIPQPCIGSLGTSLEQPEAEGDKERPVSTQLSRSSVAELSASDEDEEEIQLGDDDNDDDDEQTKDPVEEEAPKGCKRQNSQSSGNAEKRRSVEVTSGHQESVYKMDKENSEPEEIYKAVVVSSSEDEQAVLLEITDDTEPAKDDSAAHPEVLEPVENEEAESTEDSKTFSSEEEEEETPTHESTSILPPSVLDQASVIAERFISSMSRRSSMALDDGKVIGYITPRLTSRCSSLINLESTDTIIYQNGSSDFQDFSEVCGDTGVDILDTTFSKVNSASMENVFEERERPTCKRRDSILSMQDRQLIDKIRTYYDDAENRDASFSIKRRESLNYIPTGLVRNSVFKINSLPRFDTTQSSLSRQRMSSSSSNTSGVNSRHASLTDTRSNARESEQLETQTELLLETATNGTSDHSAPITDEEFRTPDEMIKVWDEFEKVNAWKEASDILEAKKDVKPDSVGECTAHCALENGLESHEPLLILEDSDLSTITEESPLPTPESTSPKQFGCVPNNTSFSEYKTPVKLHSKIMQMASCMDEDMSEKMKNKVYQLARQYSQRIKCNKPVSQRQLKEIEEDLRRNSLPAVQEEKLDKKEKRKPILSLPTYDSVILQEHGPTTPTSALSRDTAPKHLSFSPSCDSPRALSPERADCRSPLSPVKAERFHWPDVRELRSRYTSAASSSKAPPVNRSQSVPERITVPNLRGHSDTQSKGCLIHSHSMNVKHTENVNGSAEDSAQNNKAQVETEPPLPVSPSLKGKTDSLLGSAKPGYYISAEAPLENNKKVIVMEKIPAATDSNGSHDVESDDTYVQIRSPTSREKISLKAVIERCKAYQESEEYRSREVASKDGETTECTESVPHEEPNLERHVAPSCDVNQQNRVKNLREKFQTKHNSSSLKQSEVH

Target ORF information:

RefSeq Version	XM_018096660.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pleckstrin homology and RhoGEF domain containing G3 (plekhg3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096660.1

ORF Insert Sequence:

ATGGAACTGG TGGCCACTGC AGCAGAGAGC CTGCAGGCCA CCTACAGGGA GATGCAGAAC 
GGTCTGAGGA ACAACCACAC ACCCAACAAT AATTCTGCCA ATCGACGCAG CCACCAGGCG 
TCTAGGTCCC TTTCTCCATT TGGTAACAAG GTCAATGCCA TTACTCACAA ACTCAGCTAT 
GTGGAACGGG TGGTTCTGGA GATCAATGAC ACAGAAAGAA TGTATGTCCA GGATCTGCGC 
AGCATTGTGG AGGATTACCT GGGAGCAATC ATAGACAGAC AGGAAGAGCT GCCTATAACC 
CCAGAGCAAG TCTGTGCTCT CTTTGGAAAC ATTGAGGACA TTTATGAACT GAACAGTGAG 
CTGCTCCAGG ACCTGGATAA TTGCAGGAAT GACCCCGTGG CCGTGGCCAG CTGCTTTGTC 
GAAAAGAGTC AGGACTTTGA CATCTACACC CAGTACTGCA CCAACTATCC CAACTCCGTG 
GCCACCTTGA CGGAGTGCAT GAGGAATAAA ACCCTGGCCA AGTTCTTTCG AGAGAAGCAG 
GAGATGCTGA ACCAATCCCT TCCACTCGGC TCCTACCTCC TAAAGCCAGT GCAGAGAATA 
CTGAAGTATC ACCTCCTATT GCAGGAAATA GCAAAACATC TTGATGTGAA GGTGGAAGGG 
TATGAGGTGC TGGAGGAGGC TATTGAAACC ATGACGGGAG TAGCCTGGTA CATTAACGAC 
ATGAAAAGAA AGCACGAACA TGCTGTGCGG CAGCAGGAGA TCCAGTCTTT GCTGTTGAAC 
TGGAAAGGTA TAGATCTCAC TGCATATGGA GAACTTGTGC TGGAGGGAAC ATTTCGGCTC 
CAGCGAGCAC GGAGTGAGAG GACATTTTTC CTTTTCGATA AGGCCCTGTT TATCACCAAG 
AGACGTGGGG ATCACTATGT CTACAAAACT CATATCCCGT GTTCAAGCTT GATGCTGATT 
GAGAGTGCCC GTGACTCCCT CTGCTTTAGT GTGACCCATT ACAAGAACAG CAAACAGCAG 
CATAATGTGC AGGCCAAAAC AGTTGAAGAG AAACATGTGT GGACCCATCA TATAAAGAAA 
CTCATACTAG AGAACCACCA TGCAATCATT CCCCAAAAGG CGAAGGAGGC AATACTGGAA 
ATGGATAACA TGTATAAAGG AAGGTACAAA TACAGCCCAG AGAGGCAAAA GAAGACCATG 
TCTGCCATTG AGCCCTCCAT GACACACCGG TCAGGACGCA GACAGTCTGA GCCCTCCAAA 
GACATACTAA AGCAACTGAA TGACAAAGCA GGATTAAAGC ATGCAGGCAG TGATGGAGAT 
CTTCAGGAAT TTGGGGACAT TCCACAGCCT TGTATTGGCA GCTTGGGCAC CAGCCTTGAG 
CAGCCAGAGG CTGAGGGAGA CAAGGAGCGT CCTGTCAGCA CACAGCTGAG TAGAAGCTCA 
GTGGCAGAGC TCAGTGCCTC TGATGAGGAT GAGGAGGAGA TACAGTTGGG AGATGATGAT 
AATGATGATG ATGATGAGCA GACTAAAGAT CCGGTGGAGG AAGAGGCACC AAAGGGCTGC 
AAGAGACAAA ACAGCCAGTC ATCTGGGAAT GCAGAGAAGA GGAGGAGTGT GGAGGTCACA 
TCTGGGCACC AGGAGTCTGT ATATAAAATG GATAAGGAGA ATTCAGAGCC AGAAGAGATC 
TACAAGGCTG TGGTCGTCAG CTCTTCTGAG GATGAACAAG CTGTTCTGTT GGAAATCACA 
GATGACACAG AGCCAGCAAA AGATGATAGT GCAGCTCACC CAGAAGTCCT TGAGCCTGTG 
GAGAATGAAG AAGCTGAAAG TACAGAAGAC TCAAAGACTT TCAGCAGCGA AGAAGAGGAA 
GAGGAGACAC CAACCCATGA ATCAACAAGT ATCCTACCTC CTTCTGTTCT GGACCAGGCC 
AGTGTTATTG CTGAGAGGTT TATAAGCAGC ATGTCCAGGA GGAGTAGCAT GGCTCTAGAT 
GATGGGAAAG TCATAGGCTA CATCACCCCC AGATTAACCA GCCGCTGCAG TAGTTTGATC 
AATCTGGAAT CTACAGATAC TATTATTTAT CAGAACGGTT CAAGCGACTT CCAGGACTTC 
TCAGAAGTTT GTGGTGACAC TGGAGTAGAC ATTCTTGACA CCACTTTTAG TAAGGTGAAC 
TCTGCCAGTA TGGAGAATGT GTTTGAGGAA AGAGAAAGGC CCACATGTAA GAGAAGAGAC 
TCTATTCTAT CCATGCAAGA TAGACAACTC ATTGATAAAA TCAGAACTTA CTATGACGAT 
GCAGAAAATC GGGATGCATC CTTTAGCATT AAGCGCAGAG AGAGCTTGAA CTATATTCCC 
ACTGGACTTG TTAGAAACTC TGTATTTAAG ATTAACAGCC TGCCCAGATT TGATACTACC 
CAGAGTTCTC TATCGAGGCA GAGAATGTCC AGTTCATCTA GTAATACCAG CGGTGTGAAC 
AGTAGACATG CTTCCTTGAC AGATACAAGG TCTAATGCAA GAGAATCAGA GCAGTTAGAG 
ACGCAAACTG AACTGCTTTT GGAAACAGCC ACAAATGGCA CTTCTGACCA TTCAGCCCCG 
ATTACTGATG AGGAGTTCCG GACACCAGAT GAAATGATTA AAGTCTGGGA TGAGTTTGAA 
AAGGTAAATG CTTGGAAAGA GGCCTCAGAT ATTTTAGAAG CCAAAAAAGA TGTCAAACCA 
GACTCTGTGG GTGAATGTAC AGCACACTGT GCCCTGGAGA ATGGTTTGGA ATCTCACGAA 
CCACTGCTCA TATTGGAAGA CAGCGATCTG AGTACTATAA CAGAGGAATC GCCTCTTCCT 
ACACCGGAAA GCACATCTCC TAAACAGTTT GGCTGTGTTC CCAATAACAC AAGTTTTAGT 
GAGTACAAAA CGCCTGTGAA GCTACACTCC AAGATAATGC AAATGGCCAG CTGCATGGAT 
GAAGACATGT CGGAGAAGAT GAAGAACAAG GTGTACCAGC TGGCCAGGCA GTACAGTCAG 
AGGATCAAAT GCAACAAGCC AGTGTCCCAA AGGCAGCTAA AGGAGATAGA GGAGGATCTC 
AGAAGGAACA GCCTGCCTGC TGTGCAAGAG GAAAAACTGG ATAAGAAAGA GAAACGCAAG 
CCAATACTGT CACTCCCCAC ATACGACAGC GTTATACTCC AGGAGCATGG CCCTACAACC 
CCAACATCGG CCCTGAGCAG AGACACAGCT CCAAAGCATC TTTCCTTTTC ACCATCCTGT 
GACAGCCCAC GGGCTCTTTC CCCAGAGAGA GCTGACTGCA GGAGCCCTCT GAGTCCAGTC 
AAGGCAGAGA GATTTCACTG GCCTGACGTC CGAGAACTGA GATCTAGGTA CACAAGTGCA 
GCCAGCTCTA GCAAGGCTCC TCCCGTCAAC AGGAGCCAGT CTGTGCCAGA AAGAATAACT 
GTGCCCAATT TGAGGGGGCA TTCTGACACA CAAAGTAAAG GTTGCTTGAT TCACTCTCAC 
AGCATGAACG TGAAACACAC AGAAAATGTC AATGGCAGCG CTGAGGACTC GGCACAGAAT 
AACAAAGCAC AGGTAGAAAC TGAGCCGCCT TTGCCAGTGT CACCCAGTCT AAAAGGTAAA 
ACTGATTCAC TACTTGGCTC TGCAAAGCCT GGCTATTACA TCAGTGCAGA GGCTCCTTTG 
GAAAATAACA AGAAAGTTAT CGTTATGGAA AAAATTCCTG CTGCCACAGA CTCTAATGGC 
TCCCATGATG TGGAAAGCGA TGACACTTAC GTACAAATCC GATCCCCCAC AAGCAGAGAG 
AAGATCTCTC TCAAAGCCGT GATTGAGCGC TGCAAAGCCT ACCAGGAGTC CGAGGAGTAC 
CGGTCCAGGG AAGTAGCCTC CAAGGATGGG GAAACCACTG AATGCACTGA ATCTGTTCCC 
CATGAAGAGC CCAATCTGGA GAGGCACGTG GCACCAAGCT GCGATGTCAA CCAGCAGAAC 
CGAGTCAAGA ACTTGAGAGA AAAGTTTCAG ACTAAGCACA ACTCAAGTAG TTTGAAACAA 
TCGGAGGTTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	plekhg3
Entrez Gene ID	100497371
Full Name	pleckstrin homology and RhoGEF domain containing G3
Synonyms	arhgef43
General protein information	Preferred Names pleckstrin homology and RhoGEF domain containing G3 Names pleckstrin homology domain-containing family G member 3 pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	PLEKHG3	XP_002805135.1
Mus musculus (house mouse)	Plekhg3	NP_722499.4
Homo sapiens (human)	PLEKHG3	NP_056364.1
Danio rerio (zebrafish)	LOC101886323	XP_005160387.1
Xenopus tropicalis (tropical clawed frog)	LOC100497371	XP_004917341.1
Pan troglodytes (chimpanzee)	PLEKHG3	XP_510005.4
Canis lupus familiaris (dog)	PLEKHG3	XP_005623558.1
Bos taurus (cattle)	PLEKHG3	NP_001032547.2
Rattus norvegicus (Norway rat)	Plekhg3	XP_006240313.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

plekhg3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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