ambra1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43965 XM_018093274.1
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa43966 XM_018093275.1
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa56464 XM_004913386.4
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa56465 XM_004913387.4
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa56466 XM_002934098.5
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa08550 XM_004913386.3
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa08551 XM_004913387.3
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa08552 XM_002934098.4
Latest version!
Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43965
    Clone ID Related Accession (Same CDS sequence) XM_018093274.1
    Accession Version XM_018093274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC
    GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG
    GAAGGAATCC CCTCATCATC GGAGGTACCT GGCTCAGGAG AGGGCCAGGA GGATGCTCTG
    AGCAGGATCC AGAGGCTTAT GGCTGAAGGA GGGATGACGG CCGTGGTGCA GAGAGAGCAG
    AGCACAACTA TGGCATCCAT GGGAGGCTTT GGCAACAACA TCATAGTCAG CCACAGAATC
    CACCGCGGCT CCCAGACTGC GTCTGACCCG GCCTCCCGCG CTTCTGCCCC ACGCTCCCCA
    CAGCCCTCCA CCTCCAGAGA GACGGTGGCA GAGCTAGAAC GCCTTCTTGC CCCGCCCCAA
    ACGAGTCAAT TACTCCTCCC CGAGACGCAG TTGCTGACCA ATAACAATAA TGATGGTGAA
    GTCAATGTGC AGCCTCTTAG TAGTGGCACC TTTCCTGGGG TGGAGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948763.1
    CDS325..4275
    Translation

    Target ORF information:

    RefSeq Version XM_018093274.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC
    GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG
    GAAGGAATCC CCTCATCATC GGAGGTACCT GGCTCAGGAG AGGGCCAGGA GGATGCTCTG
    AGCAGGATCC AGAGGCTTAT GGCTGAAGGA GGGATGACGG CCGTGGTGCA GAGAGAGCAG
    AGCACAACTA TGGCATCCAT GGGAGGCTTT GGCAACAACA TCATAGTCAG CCACAGAATC
    CACCGCGGCT CCCAGACTGC GTCTGACCCG GCCTCCCGCG CTTCTGCCCC ACGCTCCCCA
    CAGCCCTCCA CCTCCAGAGA GACGGTGGCA GAGCTAGAAC GCCTTCTTGC CCCGCCCCAA
    ACGAGTCAAT TACTCCTCCC CGAGACGCAG TTGCTGACCA ATAACAATAA TGATGGTGAA
    GTCAATGTGC AGCCTCTTAG TAGTGGCACC TTTCCTGGGG TGGAGAGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43966
    Clone ID Related Accession (Same CDS sequence) XM_018093275.1
    Accession Version XM_018093275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC
    GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG
    GAAGGAATCC CCTCATCATC GGAGGAGAGG GCCAGGAGGA TGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948764.1
    CDS325..3912
    Translation

    Target ORF information:

    RefSeq Version XM_018093275.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018093275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC
    GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG
    GAAGGAATCC CCTCATCATC GGAGGAGAGG GCCAGGAGGA TGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56464
    Clone ID Related Accession (Same CDS sequence) XM_004913386.4
    Accession Version XM_004913386.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913386.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA
    GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG
    ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC
    AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC
    CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA
    GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG
    ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT
    GGGGTGGAGA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913443.1
    CDS320..4234
    Translation

    Target ORF information:

    RefSeq Version XM_004913386.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913386.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA
    GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG
    ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC
    AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC
    CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA
    GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG
    ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT
    GGGGTGGAGA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56465
    Clone ID Related Accession (Same CDS sequence) XM_004913387.4
    Accession Version XM_004913387.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913387.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG
    CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA
    GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG
    GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC
    ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC
    GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA
    CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC
    AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG
    GTGGAGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913444.1
    CDS321..4232
    Translation

    Target ORF information:

    RefSeq Version XM_004913387.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913387.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG
    CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA
    GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG
    GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC
    ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC
    GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA
    CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC
    AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG
    GTGGAGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56466
    Clone ID Related Accession (Same CDS sequence) XM_002934098.5
    Accession Version XM_002934098.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934098.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG
    AGGATGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934144.2
    CDS320..3871
    Translation

    Target ORF information:

    RefSeq Version XM_002934098.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934098.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG
    AGGATGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa08550
    Clone ID Related Accession (Same CDS sequence) XM_004913386.3
    Accession Version XM_004913386.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA
    GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG
    ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC
    AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC
    CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA
    GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG
    ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT
    GGGGTGGAGA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913443.1
    CDS325..4239
    Translation

    Target ORF information:

    RefSeq Version XM_004913386.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913386.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA
    GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG
    ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC
    AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC
    CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA
    GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG
    ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT
    GGGGTGGAGA GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa08551
    Clone ID Related Accession (Same CDS sequence) XM_004913387.3
    Accession Version XM_004913387.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG
    CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA
    GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG
    GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC
    ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC
    GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA
    CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC
    AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG
    GTGGAGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913444.1
    CDS325..4236
    Translation

    Target ORF information:

    RefSeq Version XM_004913387.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913387.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG
    CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA
    GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG
    GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC
    ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC
    GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA
    CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC
    AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG
    GTGGAGAGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa08552
    Clone ID Related Accession (Same CDS sequence) XM_002934098.4
    Accession Version XM_002934098.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934098.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG
    AGGATGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934144.2
    CDS325..3876
    Translation

    Target ORF information:

    RefSeq Version XM_002934098.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934098.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
    CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG
    AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT
    TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA
    GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT
    GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT
    CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC
    GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT
    CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG
    GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA
    AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT
    CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA
    CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA
    CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC
    CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA
    GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT
    GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT
    TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA
    CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT
    TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG
    GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT
    TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA
    CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG
    CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT
    AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT
    GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG
    CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA
    CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC
    AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC
    AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT
    ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC
    AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA
    AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT
    AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT
    CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT
    TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC
    GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC
    GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG
    TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA
    GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC
    AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG
    TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA
    CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG
    TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC
    AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG
    ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC
    CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA
    CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT
    CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT
    CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA
    ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT
    GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA
    AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC
    TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT
    GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC
    CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC
    CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC
    CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG
    GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG
    AGGATGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.