agap2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	agap2
Entrez Gene ID	100496997
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Synonyms	centg1,ggap2,pike
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 centaurin gamma 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911920.4	XP_004911977.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
XM_002933688.4	XP_002933734.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
XM_002933688.5	XP_002933734.3	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
XM_012957846.3	XP_012813300.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
XM_012957847.2	XP_012813301.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X4
XM_004911920.3	XP_004911977.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
XM_012957846.2	XP_012813300.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
XM_012957847.3	XP_012813301.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100496997	XP_002933734.2
Bos taurus (cattle)	AGAP2	NP_001178968.1
Rattus norvegicus (Norway rat)	Agap2	NP_075415.2
Danio rerio (zebrafish)	agap2	NP_001073487.1
Pan troglodytes (chimpanzee)	AGAP2	XP_509171.3
Mus musculus (house mouse)	Agap2	NP_001028435.1
	AGAP2	XP_001116285.1
Homo sapiens (human)	AGAP2	NP_001116244.1

Gene Ontology

The following agap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36737	XM_012957846.2 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa36738	XM_012957847.2 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa52702	XM_012957846.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa52703	XM_012957847.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa52700	XM_002933688.5 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa52701	XM_004911920.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa06078	XM_002933688.4 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OXa06079	XM_004911920.3 Latest version!	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

agap2 ( XM_012957846.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813300.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa36737
Clone ID Related Accession (Same CDS sequence)	XM_012957846.2
Accession Version	XM_012957846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GCCAAGCGCA AAATGTGGAA GTTAAAAACA TTTGGTAGCT TGAGAAATAT TTATAAGACA 
GAAGAGAACT TCGAATTTAT CATTGTGTCT AGCACAGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ACAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG CATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813300.1
CDS	438..2924
Translation	MSGEFYRGRDGIHGSLKMSSGKQQMSAAIRGEVKRHEVAKAQLTRLLKLAEQVSEEHLKDNLVVSLRSLRECVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADAKRKMWKLKTFGSLRNIYKTEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_012957846.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957846.2

ORF Insert Sequence:

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GCCAAGCGCA AAATGTGGAA GTTAAAAACA TTTGGTAGCT TGAGAAATAT TTATAAGACA 
GAAGAGAACT TCGAATTTAT CATTGTGTCT AGCACAGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ACAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG CATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_012957847.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813301.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa36738
Clone ID Related Accession (Same CDS sequence)	XM_012957847.2
Accession Version	XM_012957847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GAAGAGAACT TCGAATTTAT CATTGTGTCT AGCACAGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ACAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG CATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813301.1
CDS	438..2864
Translation	MSGEFYRGRDGIHGSLKMSSGKQQMSAAIRGEVKRHEVAKAQLTRLLKLAEQVSEEHLKDNLVVSLRSLRECVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_012957847.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957847.2

ORF Insert Sequence:

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GAAGAGAACT TCGAATTTAT CATTGTGTCT AGCACAGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ACAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG CATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_012957846.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813300.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52702
Clone ID Related Accession (Same CDS sequence)	XM_012957846.3
Accession Version	XM_012957846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957846.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GCCAAGCGCA AAATGTGGAA GTTAAAAACA TTTGGTAGCT TGAGAAATAT TTATAAGACA 
GAAGAGAACT TCGAATTCAT CATTGTGTCT AGCACCGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ATAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG TATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813300.1
CDS	332..2818
Translation	MSGEFYRGRDGIHGSLKMSSGKQQMSAAIRGEVKRHEVAKAQLTRLLKLAEQVSEEHLKDNLVVSLRSLRECVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADAKRKMWKLKTFGSLRNIYKTEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_012957846.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957846.3

ORF Insert Sequence:

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GCCAAGCGCA AAATGTGGAA GTTAAAAACA TTTGGTAGCT TGAGAAATAT TTATAAGACA 
GAAGAGAACT TCGAATTCAT CATTGTGTCT AGCACCGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ATAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG TATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_012957847.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813301.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52703
Clone ID Related Accession (Same CDS sequence)	XM_012957847.3
Accession Version	XM_012957847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GAAGAGAACT TCGAATTCAT CATTGTGTCT AGCACCGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ATAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG TATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813301.1
CDS	333..2759
Translation	MSGEFYRGRDGIHGSLKMSSGKQQMSAAIRGEVKRHEVAKAQLTRLLKLAEQVSEEHLKDNLVVSLRSLRECVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_012957847.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957847.3

ORF Insert Sequence:

ATGAGCGGGG AGTTTTACAG AGGCAGAGAT GGCATTCATG GAAGTCTCAA GATGAGTTCA 
GGAAAGCAGC AAATGTCTGC AGCCATTCGA GGTGAAGTGA AGAGACATGA GGTGGCCAAG 
GCTCAGCTCA CTAGGCTGCT CAAACTAGCA GAGCAAGTGA GCGAAGAGCA TCTGAAAGAT 
AACCTAGTCG TCTCCCTGCG GAGCCTGAGA GAATGTGTGA TGAACAGCCA GGAATGGACC 
TTAAGTCGGA CTGTGCCTGA AATCAGAGTG GGAGTTTTGG GCAGTACTAA AAGTGCCAAG 
TCTGCTCTTG TGCATCGGTT TGTGACCGGC TCCTATCAAC CCCTAGAAGC ACCTGAAAGT 
GCTCAGTTCA AGAAAGAGAT CAGCGTGGAA GGGCAGAGCT ACCTTCTGCT AATTAGGGAA 
GAAGCCGGAG CTCCAGATGC CAAGTTTGCC AATTGGGTGG ATGCCGTCAT TTTTGTGTTC 
AGCCTGGAAA ATGAGGCAAG CTTTCAAGAT ATTTTCCAGT ATTATGCTCT CCTGGCAAAT 
TATCGCAATG TTGCAGACCT CGCTATGGCA CTTGTTGGGA CTCAAGATAA AATTAGTGCA 
AACAACAAGC GAGTGGTAGA GGACGTGCGG GCGAGAGCCC TCTGCAATGA TATCAAACGC 
TGTGTTTACT ATGAGACCTG TGCCACCTAT GGACTCAATG TGGATCGGGT GTTTAATGAA 
GTTGCTCAGA AGATGGTTGC AATAAAGAGG CAGCAGCAAC TCCTATCATG CAAGTCATTA 
CCAAGCTCTC CAAGTCACTC CTCTGGATCC ATTGCAATAT CAGTGCAGGC AAGTAATGGA 
GGAACAACAA GTGATTATTC ATCGTCACTG CCTTCCACAC CAAACATCAG TCACAGAGAA 
CTACGAAGTG AGGTGTCCAT TGGAGTCAGC ACTCCGGTGT CCCTCCATCG TGGCACTAAA 
AGGCGCACAA GCATATTTAC AAACCGAAGG GGAAGTGATT TGGAAAAGAG AAGTTTTGAT 
AGTCGTGCAG ATGGTGCTGG TGGGGGTCGG GCTCTACCAA TTAAACAGAG CTTCTTAATG 
AAACGAAGCG GCAACTCTCT GAACAAAGAA TGGAAGAAGA AGTATGTTAC CCTGACAAGC 
AATGGGGTCC TGCTCTACCA TCCAAGCATT AATGATTACC TACACAGTAC ACATGGGAAG 
GAGATGGATC TTTTACGAAC TACAGTGAAG GTCCCTGGAA AGAGGCCTCC AATAGCCATG 
TCTGCCTGTG GCCCATCCAA TAGCATCAAC GGCTTGTTAA AGGATGTGGG CAGCTCTCCA 
GGGGGAGATA CAGCAAGTAA CAAGGCGCAA TCAACAAAAA GCAAATCCAC TGGCGATGTC 
ATTTCCAGCC CCACCAGCAA CAAAGATCCC CCCTCTTCCC CAATGAGTGA TAAAAAGAAG 
AACAGAAGAA AAAAGAATAT GACCCCTTCC AAGACAGAAG GGTCCGCTGG ACAGGCAGAT 
GAAGAGAACT TCGAATTCAT CATTGTGTCT AGCACCGGGC AGACATGGCA TTTTGAGACA 
AGCAGCTTTG AAGAAAGGGA TTCCTGGGTA CAAGCCATTG AGAGCCAGAT CCTCGCTAGC 
CTGCAGTGCT GCGAGAGCAG CAAAAATAAG GCTCGAATGG ATAGTCAGAG CGAAGCGGTC 
GCCATCCAAG CCATTCGGAA TGCTAAAGGG AATTCATTTT GTGTGGATTG TGGTGCCCCG 
AATCCCACCT GGGCCAGCTT GAATCTGGGT GCCCTGATCT GCATTGAATG TTCAGGAATT 
CACAGAAACC TTGGCACCCA TTTATCTCGG GTCCGATCAC TGGATTTGGA TGACTGGCCC 
CTAGAGCTCA CACTTGTTCT AACCTCTATT GGTAATGAAA TGGCCAACAG TATATGGGAG 
ATGACAACTC ATGGAAGAAC CAAACCAGCA CCTGATTCCT CAAGGGAAGA GAGAGAATCA 
TGGATTAGAG CTAAGTATGA GCAGCGATTA TTTTTGGCCC CTTTGAAGAG TCCAGGGGTC 
CCCTTGGTGA AGCAGCTTTT TAAAGCTGTA CATGAGAAAA ATCTCGGCAA TGTACTTCTC 
CTCCTAGCCC ACAGCAACAA GGAGCAAATC AACGGAATCA CTGGAGACAG AGACAGACGC 
ACTGCCCTTC ATGTGGCCTG TGAACTGGGG GAAGTTGTGA CCACACAACT GCTTGTCTGG 
TATGGCATCG ATGTGAAGTC AAGAGACAGC AATGGCCACA CAGCCGTGTT TTATGCTAAG 
AAAAGTAACA GCCAAGAGTG CGTGGACATT CTGCTGCAAC ATGGCTGTCC CAATGAGTCC 
ACAAGCGCCC TGTCCACTCC CAGTCTAAGA AGGAAAAGCA GCTCTGCCAG CATCGTGAGG 
ACTGATTCCC GGACTGCCCT TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_002933688.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933734.3 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.

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CloneID	OXa52700
Clone ID Related Accession (Same CDS sequence)	XM_002933688.5
Accession Version	XM_002933688.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933688.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCAGT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC CTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGCC AAGCGCAAAA TGTGGAAGTT AAAAACATTT 
GGTAGCTTGA GAAATATTTA TAAGACAGAA GAGAACTTCG AATTCATCAT TGTGTCTAGC 
ACCGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGATA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGTAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933734.3
CDS	604..6717
Translation	MSRGATLQRRTTYHISLTLVKLEAVGEEKKGETREVYRRHDTPSLGSTPGLAHCLSLDREFPDAWVWGGPDAASRVRDQPSPGAGSAAAGDGSAAGTKVRGKVIRSQTQAPVPSKPTFPGHGQTATPGTAVPRPVPKGAAVPTSGPKGSGTSGSAKGTSSPAACAIDSRARTAATEEPGSKGTFRSAAPVPASKEASPSSGTPAPGPKPSNVASTARTAPQSTSSRPPSHAAPPPPHKHNGTSDPAALALTPSHPPLAPTAKDSGTSSPASLASIPKDSSAPDMASLAPNLTYNNTPSPSASIPLDSCAPCPSGPNLSAKESVSPAPTPSAPGQAGPVSKAKDTDMSGLAAPGKDTGRPGATCSPMTPAASAPKMKEKDAPRPTATASKVKEICTAEMTPVTEAASVSFVPAAKGSYTSATAALVPTSKGTGLSSSEGSVPTSKSYGTSIPATPVPPPLPMGNVTLISDPSAPTPKGNSQFVPAAPTTTPKCAGQSFPAAPTPKGRGQSISAAPAPTPKVSGTSISAAPTSTLKGSSSICTATTIPKASGISSLGTSVPTSKESNTLNSAAPSKDSGKSSPADPSTILQDKDVLHSADQVPTAAPSDKSSPDVPTPTAEDSNKKSSEPQLAEDKVITATSNSGPVKDTGTSSTATTVPAGQREFTFGTARVIRGSLIRRPALDTDVSRDLVSSPTVPVQGARITEGVNAEAPAIEARVSSRSFKAVRIPAGSTETGTASRANRASVKVEKPSKASEPEDKPCPVMTSVPRVTAPPISAARTAAALFSASRSGAPHNARPSEAKITAIRTAARGARAVSSGACTEDLAGRRTPNTGLGERAQTGDAGTYPGAKASVMGPKTATGTSPVKVAKIAPRPMSSCADPIIITGLRTKASTAVKAPLSGTGTYPGAKSSLSGSFTSLGSKALTDSSGIVAPSAEEKFSPGLVSPHVVRTPVPSAFSLPIRPEDILPGGEEEFTPDNEDPAKSKRQERLFLRQDAMWISTSVTSDYFLQSSPGRPGSGGSSPGGHRQELSPKFFSGPRCSFSSPGSPLLTAPCQPGTPYREAPINVSLTVSQEHAGGSSESCLLIERSASSGVSSVGVPEAGELGKGHPALLTPGKEKGGRILKLKASKSQVEKPTEQRDKLQSQKSFTNRLSWPENEGGKSRSKSSTKSSPRVETPKLSGMAGKNLLSPASCDPKGFRKGKSKTVDNSDLDPTAEDFKKSREGSSPTGDKARATAARDRKMLKFISGIFAKNTASVASSVSPSSLSPGDERDILHKCVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADAKRKMWKLKTFGSLRNIYKTEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_002933688.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933688.5

ORF Insert Sequence:

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCAGT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC CTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGCC AAGCGCAAAA TGTGGAAGTT AAAAACATTT 
GGTAGCTTGA GAAATATTTA TAAGACAGAA GAGAACTTCG AATTCATCAT TGTGTCTAGC 
ACCGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGATA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGTAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_004911920.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911977.2 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.

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CloneID	OXa52701
Clone ID Related Accession (Same CDS sequence)	XM_004911920.4
Accession Version	XM_004911920.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911920.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCAGT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC CTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGAA GAGAACTTCG AATTCATCAT TGTGTCTAGC 
ACCGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGATA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGTAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911977.2
CDS	606..6659
Translation	MSRGATLQRRTTYHISLTLVKLEAVGEEKKGETREVYRRHDTPSLGSTPGLAHCLSLDREFPDAWVWGGPDAASRVRDQPSPGAGSAAAGDGSAAGTKVRGKVIRSQTQAPVPSKPTFPGHGQTATPGTAVPRPVPKGAAVPTSGPKGSGTSGSAKGTSSPAACAIDSRARTAATEEPGSKGTFRSAAPVPASKEASPSSGTPAPGPKPSNVASTARTAPQSTSSRPPSHAAPPPPHKHNGTSDPAALALTPSHPPLAPTAKDSGTSSPASLASIPKDSSAPDMASLAPNLTYNNTPSPSASIPLDSCAPCPSGPNLSAKESVSPAPTPSAPGQAGPVSKAKDTDMSGLAAPGKDTGRPGATCSPMTPAASAPKMKEKDAPRPTATASKVKEICTAEMTPVTEAASVSFVPAAKGSYTSATAALVPTSKGTGLSSSEGSVPTSKSYGTSIPATPVPPPLPMGNVTLISDPSAPTPKGNSQFVPAAPTTTPKCAGQSFPAAPTPKGRGQSISAAPAPTPKVSGTSISAAPTSTLKGSSSICTATTIPKASGISSLGTSVPTSKESNTLNSAAPSKDSGKSSPADPSTILQDKDVLHSADQVPTAAPSDKSSPDVPTPTAEDSNKKSSEPQLAEDKVITATSNSGPVKDTGTSSTATTVPAGQREFTFGTARVIRGSLIRRPALDTDVSRDLVSSPTVPVQGARITEGVNAEAPAIEARVSSRSFKAVRIPAGSTETGTASRANRASVKVEKPSKASEPEDKPCPVMTSVPRVTAPPISAARTAAALFSASRSGAPHNARPSEAKITAIRTAARGARAVSSGACTEDLAGRRTPNTGLGERAQTGDAGTYPGAKASVMGPKTATGTSPVKVAKIAPRPMSSCADPIIITGLRTKASTAVKAPLSGTGTYPGAKSSLSGSFTSLGSKALTDSSGIVAPSAEEKFSPGLVSPHVVRTPVPSAFSLPIRPEDILPGGEEEFTPDNEDPAKSKRQERLFLRQDAMWISTSVTSDYFLQSSPGRPGSGGSSPGGHRQELSPKFFSGPRCSFSSPGSPLLTAPCQPGTPYREAPINVSLTVSQEHAGGSSESCLLIERSASSGVSSVGVPEAGELGKGHPALLTPGKEKGGRILKLKASKSQVEKPTEQRDKLQSQKSFTNRLSWPENEGGKSRSKSSTKSSPRVETPKLSGMAGKNLLSPASCDPKGFRKGKSKTVDNSDLDPTAEDFKKSREGSSPTGDKARATAARDRKMLKFISGIFAKNTASVASSVSPSSLSPGDERDILHKCVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_004911920.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911920.4

ORF Insert Sequence:

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCAGT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC CTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGAA GAGAACTTCG AATTCATCAT TGTGTCTAGC 
ACCGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGATA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGTAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_002933688.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933734.2 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.

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CloneID	OXa06078
Clone ID Related Accession (Same CDS sequence)	XM_002933688.4
Accession Version	XM_002933688.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933688.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCACT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC GTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGCC AAGCGCAAAA TGTGGAAGTT AAAAACATTT 
GGTAGCTTGA GAAATATTTA TAAGACAGAA GAGAACTTCG AATTTATCAT TGTGTCTAGC 
ACAGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGACA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGCAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933734.2
CDS	888..7001
Translation	MSRGATLQRRTTYHISLTLVKLEAVGEEKKGETREVYRRHDTPSLGSTPGLAHCLSLDREFPDAWVWGGPDAASRVRDQPSPGAGSAAAGDGSAAGTKVRGKVIRSQTQAPVPSKPTFPGHGQTATPGTAVPRPVPKGAAVPTSGPKGSGTSGSAKGTSSPAACAIDSRARTAATEEPGSKGTFRSAAPVPASKEASPSTGTPAPGPKPSNVASTARTAPQSTSSRPPSHAAPPPPHKHNGTSDPAALALTPSHPPLAPTAKDSGTSSPASLASIPKDSSAPDMASLAPNVTYNNTPSPSASIPLDSCAPCPSGPNLSAKESVSPAPTPSAPGQAGPVSKAKDTDMSGLAAPGKDTGRPGATCSPMTPAASAPKMKEKDAPRPTATASKVKEICTAEMTPVTEAASVSFVPAAKGSYTSATAALVPTSKGTGLSSSEGSVPTSKSYGTSIPATPVPPPLPMGNVTLISDPSAPTPKGNSQFVPAAPTTTPKCAGQSFPAAPTPKGRGQSISAAPAPTPKVSGTSISAAPTSTLKGSSSICTATTIPKASGISSLGTSVPTSKESNTLNSAAPSKDSGKSSPADPSTILQDKDVLHSADQVPTAAPSDKSSPDVPTPTAEDSNKKSSEPQLAEDKVITATSNSGPVKDTGTSSTATTVPAGQREFTFGTARVIRGSLIRRPALDTDVSRDLVSSPTVPVQGARITEGVNAEAPAIEARVSSRSFKAVRIPAGSTETGTASRANRASVKVEKPSKASEPEDKPCPVMTSVPRVTAPPISAARTAAALFSASRSGAPHNARPSEAKITAIRTAARGARAVSSGACTEDLAGRRTPNTGLGERAQTGDAGTYPGAKASVMGPKTATGTSPVKVAKIAPRPMSSCADPIIITGLRTKASTAVKAPLSGTGTYPGAKSSLSGSFTSLGSKALTDSSGIVAPSAEEKFSPGLVSPHVVRTPVPSAFSLPIRPEDILPGGEEEFTPDNEDPAKSKRQERLFLRQDAMWISTSVTSDYFLQSSPGRPGSGGSSPGGHRQELSPKFFSGPRCSFSSPGSPLLTAPCQPGTPYREAPINVSLTVSQEHAGGSSESCLLIERSASSGVSSVGVPEAGELGKGHPALLTPGKEKGGRILKLKASKSQVEKPTEQRDKLQSQKSFTNRLSWPENEGGKSRSKSSTKSSPRVETPKLSGMAGKNLLSPASCDPKGFRKGKSKTVDNSDLDPTAEDFKKSREGSSPTGDKARATAARDRKMLKFISGIFAKNTASVASSVSPSSLSPGDERDILHKCVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADAKRKMWKLKTFGSLRNIYKTEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_002933688.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933688.4

ORF Insert Sequence:

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCACT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC GTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGCC AAGCGCAAAA TGTGGAAGTT AAAAACATTT 
GGTAGCTTGA GAAATATTTA TAAGACAGAA GAGAACTTCG AATTTATCAT TGTGTCTAGC 
ACAGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGACA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGCAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agap2 ( XM_004911920.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911977.1 Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.

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CloneID	OXa06079
Clone ID Related Accession (Same CDS sequence)	XM_004911920.3
Accession Version	XM_004911920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCACT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC GTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGAA GAGAACTTCG AATTTATCAT TGTGTCTAGC 
ACAGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGACA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGCAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911977.1
CDS	888..6941
Translation	MSRGATLQRRTTYHISLTLVKLEAVGEEKKGETREVYRRHDTPSLGSTPGLAHCLSLDREFPDAWVWGGPDAASRVRDQPSPGAGSAAAGDGSAAGTKVRGKVIRSQTQAPVPSKPTFPGHGQTATPGTAVPRPVPKGAAVPTSGPKGSGTSGSAKGTSSPAACAIDSRARTAATEEPGSKGTFRSAAPVPASKEASPSTGTPAPGPKPSNVASTARTAPQSTSSRPPSHAAPPPPHKHNGTSDPAALALTPSHPPLAPTAKDSGTSSPASLASIPKDSSAPDMASLAPNVTYNNTPSPSASIPLDSCAPCPSGPNLSAKESVSPAPTPSAPGQAGPVSKAKDTDMSGLAAPGKDTGRPGATCSPMTPAASAPKMKEKDAPRPTATASKVKEICTAEMTPVTEAASVSFVPAAKGSYTSATAALVPTSKGTGLSSSEGSVPTSKSYGTSIPATPVPPPLPMGNVTLISDPSAPTPKGNSQFVPAAPTTTPKCAGQSFPAAPTPKGRGQSISAAPAPTPKVSGTSISAAPTSTLKGSSSICTATTIPKASGISSLGTSVPTSKESNTLNSAAPSKDSGKSSPADPSTILQDKDVLHSADQVPTAAPSDKSSPDVPTPTAEDSNKKSSEPQLAEDKVITATSNSGPVKDTGTSSTATTVPAGQREFTFGTARVIRGSLIRRPALDTDVSRDLVSSPTVPVQGARITEGVNAEAPAIEARVSSRSFKAVRIPAGSTETGTASRANRASVKVEKPSKASEPEDKPCPVMTSVPRVTAPPISAARTAAALFSASRSGAPHNARPSEAKITAIRTAARGARAVSSGACTEDLAGRRTPNTGLGERAQTGDAGTYPGAKASVMGPKTATGTSPVKVAKIAPRPMSSCADPIIITGLRTKASTAVKAPLSGTGTYPGAKSSLSGSFTSLGSKALTDSSGIVAPSAEEKFSPGLVSPHVVRTPVPSAFSLPIRPEDILPGGEEEFTPDNEDPAKSKRQERLFLRQDAMWISTSVTSDYFLQSSPGRPGSGGSSPGGHRQELSPKFFSGPRCSFSSPGSPLLTAPCQPGTPYREAPINVSLTVSQEHAGGSSESCLLIERSASSGVSSVGVPEAGELGKGHPALLTPGKEKGGRILKLKASKSQVEKPTEQRDKLQSQKSFTNRLSWPENEGGKSRSKSSTKSSPRVETPKLSGMAGKNLLSPASCDPKGFRKGKSKTVDNSDLDPTAEDFKKSREGSSPTGDKARATAARDRKMLKFISGIFAKNTASVASSVSPSSLSPGDERDILHKCVMNSQEWTLSRTVPEIRVGVLGSTKSAKSALVHRFVTGSYQPLEAPESAQFKKEISVEGQSYLLLIREEAGAPDAKFANWVDAVIFVFSLENEASFQDIFQYYALLANYRNVADLAMALVGTQDKISANNKRVVEDVRARALCNDIKRCVYYETCATYGLNVDRVFNEVAQKMVAIKRQQQLLSCKSLPSSPSHSSGSIAISVQASNGGTTSDYSSSLPSTPNISHRELRSEVSIGVSTPVSLHRGTKRRTSIFTNRRGSDLEKRSFDSRADGAGGGRALPIKQSFLMKRSGNSLNKEWKKKYVTLTSNGVLLYHPSINDYLHSTHGKEMDLLRTTVKVPGKRPPIAMSACGPSNSINGLLKDVGSSPGGDTASNKAQSTKSKSTGDVISSPTSNKDPPSSPMSDKKKNRRKKNMTPSKTEGSAGQADEENFEFIIVSSTGQTWHFETSSFEERDSWVQAIESQILASLQCCESSKNKARMDSQSEAVAIQAIRNAKGNSFCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPLELTLVLTSIGNEMANSIWEMTTHGRTKPAPDSSREERESWIRAKYEQRLFLAPLKSPGVPLVKQLFKAVHEKNLGNVLLLLAHSNKEQINGITGDRDRRTALHVACELGEVVTTQLLVWYGIDVKSRDSNGHTAVFYAKKSNSQECVDILLQHGCPNESTSALSTPSLRRKSSSASIVRTDSRTALV

Target ORF information:

RefSeq Version	XM_004911920.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (agap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911920.3

ORF Insert Sequence:

ATGAGCAGAG GAGCCACCCT GCAGCGCAGG ACCACCTACC ATATATCTCT TACTCTGGTC 
AAACTAGAGG CTGTAGGAGA AGAAAAGAAG GGAGAGACTC GGGAAGTCTA TCGGAGACAT 
GACACCCCGT CTCTCGGCAG CACCCCCGGT CTGGCCCATT GCCTCTCTCT GGACAGGGAA 
TTCCCAGATG CCTGGGTGTG GGGTGGGCCC GATGCTGCTT CCAGGGTGAG AGATCAGCCT 
AGTCCGGGAG CCGGTTCCGC AGCGGCCGGG GATGGCTCTG CCGCAGGGAC AAAGGTGAGA 
GGCAAGGTGA TCCGGAGCCA GACCCAAGCG CCTGTGCCCA GCAAGCCAAC TTTCCCCGGC 
CATGGGCAGA CTGCTACACC CGGCACTGCT GTGCCTCGGC CGGTACCGAA AGGAGCCGCG 
GTTCCCACAT CTGGACCTAA AGGGAGCGGT ACCTCCGGCT CTGCCAAGGG AACATCCAGC 
CCCGCAGCCT GTGCCATAGA CTCCCGGGCA CGTACCGCGG CCACTGAGGA ACCTGGCAGC 
AAAGGCACTT TCAGATCTGC CGCCCCGGTA CCGGCGAGTA AAGAAGCCTC TCCCAGCACT 
GGGACACCGG CACCGGGACC CAAGCCCAGC AATGTAGCCT CAACTGCTCG TACAGCGCCT 
CAATCTACTT CCAGCCGCCC CCCCAGTCAT GCAGCCCCGC CACCTCCTCA CAAACACAAT 
GGCACCTCTG ATCCAGCTGC CTTGGCACTC ACACCGTCAC ATCCACCCTT GGCACCAACA 
GCAAAGGATA GTGGCACTTC ATCGCCTGCA TCCTTGGCAT CTATACCAAA GGACAGCAGT 
GCACCAGATA TGGCATCCCT GGCTCCTAAC GTTACATACA ATAATACCCC AAGCCCTTCA 
GCCTCTATAC CTTTAGACAG CTGTGCACCA TGCCCTTCAG GCCCTAATCT GTCAGCTAAG 
GAAAGTGTCA GTCCAGCTCC TACACCAAGT GCACCAGGAC AAGCAGGTCC AGTTTCCAAA 
GCCAAGGACA CTGACATGTC TGGTCTTGCA GCCCCAGGTA AGGACACTGG GAGACCTGGT 
GCCACCTGTA GTCCTATGAC ACCTGCAGCA TCAGCACCAA AAATGAAGGA AAAGGACGCA 
CCAAGACCTA CAGCAACAGC ATCCAAAGTT AAGGAAATTT GTACTGCAGA AATGACACCT 
GTAACAGAAG CTGCTTCTGT AAGTTTTGTT CCAGCAGCTA AGGGCAGCTA CACATCAGCT 
ACTGCAGCTC TGGTACCCAC ATCAAAGGGC ACTGGCTTAT CAAGTTCTGA AGGCTCTGTA 
CCCACATCAA AGAGCTATGG CACATCAATA CCTGCAACTC CAGTACCACC ACCATTACCT 
ATGGGCAATG TCACATTAAT TTCTGACCCT TCAGCACCCA CACCAAAGGG CAATAGCCAG 
TTTGTTCCTG CAGCTCCAAC AACCACACCT AAGTGCGCTG GCCAATCATT TCCTGCAGCT 
CCAACACCTA AGGGCAGGGG ACAATCAATT TCTGCAGCTC CAGCACCCAC ACCTAAGGTC 
AGTGGGACAT CAATTTCTGC AGCTCCAACA TCCACTCTTA AGGGCAGCTC ATCAATTTGT 
ACTGCAACAA CCATACCCAA GGCCAGTGGT ATATCAAGCC TTGGAACCTC GGTACCCACA 
TCTAAAGAAA GTAACACCTT AAATTCTGCA GCACCATCAA AGGACAGTGG CAAATCAAGT 
CCTGCAGACC CATCAACAAT TCTTCAGGAC AAAGATGTTT TACATTCTGC TGACCAGGTA 
CCTACAGCTG CTCCTAGTGA TAAATCAAGT CCTGACGTCC CAACTCCAAC CGCAGAAGAT 
AGTAATAAAA AGAGTTCTGA ACCCCAGTTA GCAGAGGATA AGGTTATCAC TGCAACTAGT 
AATTCAGGCC CAGTCAAAGA CACTGGCACC TCCAGCACTG CCACAACAGT GCCAGCAGGA 
CAGAGAGAGT TTACCTTTGG AACAGCTCGG GTGATCCGAG GATCCCTAAT CAGAAGACCA 
GCCTTGGATA CTGATGTGTC GCGAGATTTA GTCAGCAGTC CTACTGTTCC TGTACAAGGG 
GCCCGGATAA CAGAAGGAGT TAATGCGGAG GCCCCAGCCA TTGAAGCTAG AGTCTCTTCA 
CGAAGCTTCA AGGCTGTGAG AATTCCAGCG GGTTCAACTG AGACTGGAAC TGCATCAAGG 
GCAAATAGAG CCTCAGTCAA GGTAGAAAAG CCCAGTAAAG CATCTGAGCC AGAGGACAAG 
CCATGCCCAG TCATGACCTC TGTTCCCCGG GTAACTGCTC CACCTATCAG TGCTGCCAGA 
ACAGCAGCCG CCCTGTTTTC TGCATCTCGA TCAGGAGCCC CTCATAATGC AAGACCCAGT 
GAGGCAAAAA TAACTGCTAT CCGAACCGCA GCTCGCGGAG CCAGAGCAGT TTCTTCAGGG 
GCATGTACTG AGGACCTTGC AGGAAGAAGA ACACCAAACA CAGGCTTAGG GGAAAGAGCT 
CAAACCGGAG ATGCAGGAAC CTATCCAGGG GCAAAAGCAT CTGTCATGGG ACCAAAAACA 
GCAACTGGGA CATCACCTGT AAAAGTTGCA AAAATTGCAC CCAGGCCCAT GAGCTCTTGT 
GCAGACCCTA TAATCATCAC TGGATTGCGA ACAAAGGCCT CAACTGCTGT AAAAGCACCA 
CTGTCTGGTA CAGGTACATA CCCAGGGGCC AAATCATCTC TCTCCGGGTC ATTCACATCC 
CTGGGAAGTA AAGCATTGAC TGATAGCTCA GGAATTGTGG CTCCATCCGC AGAAGAAAAG 
TTTAGTCCAG GGTTAGTTTC ACCACATGTT GTCCGAACTC CAGTCCCCTC TGCCTTCTCC 
CTCCCAATCA GGCCTGAGGA CATTTTGCCT GGGGGGGAGG AGGAGTTTAC CCCCGATAAT 
GAGGATCCTG CTAAGAGTAA GCGCCAGGAG CGGCTGTTCC TAAGGCAGGA TGCCATGTGG 
ATCAGCACAA GTGTTACTTC AGACTATTTC TTACAGTCAT CCCCTGGACG GCCTGGTAGT 
GGTGGAAGCT CCCCTGGAGG GCACCGCCAG GAGCTTAGCC CAAAATTCTT TTCAGGGCCT 
CGCTGTTCGT TTAGCTCCCC AGGCAGCCCA CTGCTTACTG CTCCTTGTCA GCCAGGCACA 
CCTTACAGGG AGGCTCCAAT CAATGTGTCC CTTACAGTAA GCCAAGAGCA TGCTGGGGGA 
TCTTCAGAAA GTTGCCTTCT GATTGAGCGT AGTGCCTCTT CAGGGGTGAG TTCAGTAGGA 
GTTCCTGAGG CAGGTGAGCT TGGTAAAGGA CACCCTGCCC TACTTACCCC TGGTAAAGAA 
AAGGGCGGTA GAATTCTGAA ACTTAAAGCT TCTAAAAGCC AGGTAGAGAA ACCAACAGAG 
CAACGAGATA AACTTCAAAG CCAGAAGAGT TTCACTAACA GGCTTTCTTG GCCTGAAAAT 
GAGGGGGGTA AGAGCCGCTC TAAATCCTCC ACAAAGAGCA GCCCTAGAGT GGAGACACCC 
AAACTGTCTG GCATGGCAGG CAAGAACCTA TTGTCCCCTG CCTCATGTGA CCCTAAGGGT 
TTTCGGAAGG GGAAAAGTAA GACTGTGGAC AATAGTGACT TGGACCCCAC TGCAGAGGAT 
TTCAAAAAAA GCAGAGAGGG AAGCTCACCG ACAGGGGACA AGGCCAGGGC CACAGCAGCC 
AGGGATAGGA AAATGCTTAA ATTCATCAGT GGGATCTTTG CCAAGAACAC TGCATCCGTG 
GCCTCATCCG TTTCACCTAG TAGTTTAAGC CCAGGAGATG AGAGGGATAT ACTTCACAAA 
TGTGTGATGA ACAGCCAGGA ATGGACCTTA AGTCGGACTG TGCCTGAAAT CAGAGTGGGA 
GTTTTGGGCA GTACTAAAAG TGCCAAGTCT GCTCTTGTGC ATCGGTTTGT GACCGGCTCC 
TATCAACCCC TAGAAGCACC TGAAAGTGCT CAGTTCAAGA AAGAGATCAG CGTGGAAGGG 
CAGAGCTACC TTCTGCTAAT TAGGGAAGAA GCCGGAGCTC CAGATGCCAA GTTTGCCAAT 
TGGGTGGATG CCGTCATTTT TGTGTTCAGC CTGGAAAATG AGGCAAGCTT TCAAGATATT 
TTCCAGTATT ATGCTCTCCT GGCAAATTAT CGCAATGTTG CAGACCTCGC TATGGCACTT 
GTTGGGACTC AAGATAAAAT TAGTGCAAAC AACAAGCGAG TGGTAGAGGA CGTGCGGGCG 
AGAGCCCTCT GCAATGATAT CAAACGCTGT GTTTACTATG AGACCTGTGC CACCTATGGA 
CTCAATGTGG ATCGGGTGTT TAATGAAGTT GCTCAGAAGA TGGTTGCAAT AAAGAGGCAG 
CAGCAACTCC TATCATGCAA GTCATTACCA AGCTCTCCAA GTCACTCCTC TGGATCCATT 
GCAATATCAG TGCAGGCAAG TAATGGAGGA ACAACAAGTG ATTATTCATC GTCACTGCCT 
TCCACACCAA ACATCAGTCA CAGAGAACTA CGAAGTGAGG TGTCCATTGG AGTCAGCACT 
CCGGTGTCCC TCCATCGTGG CACTAAAAGG CGCACAAGCA TATTTACAAA CCGAAGGGGA 
AGTGATTTGG AAAAGAGAAG TTTTGATAGT CGTGCAGATG GTGCTGGTGG GGGTCGGGCT 
CTACCAATTA AACAGAGCTT CTTAATGAAA CGAAGCGGCA ACTCTCTGAA CAAAGAATGG 
AAGAAGAAGT ATGTTACCCT GACAAGCAAT GGGGTCCTGC TCTACCATCC AAGCATTAAT 
GATTACCTAC ACAGTACACA TGGGAAGGAG ATGGATCTTT TACGAACTAC AGTGAAGGTC 
CCTGGAAAGA GGCCTCCAAT AGCCATGTCT GCCTGTGGCC CATCCAATAG CATCAACGGC 
TTGTTAAAGG ATGTGGGCAG CTCTCCAGGG GGAGATACAG CAAGTAACAA GGCGCAATCA 
ACAAAAAGCA AATCCACTGG CGATGTCATT TCCAGCCCCA CCAGCAACAA AGATCCCCCC 
TCTTCCCCAA TGAGTGATAA AAAGAAGAAC AGAAGAAAAA AGAATATGAC CCCTTCCAAG 
ACAGAAGGGT CCGCTGGACA GGCAGATGAA GAGAACTTCG AATTTATCAT TGTGTCTAGC 
ACAGGGCAGA CATGGCATTT TGAGACAAGC AGCTTTGAAG AAAGGGATTC CTGGGTACAA 
GCCATTGAGA GCCAGATCCT CGCTAGCCTG CAGTGCTGCG AGAGCAGCAA AAATAAGGCT 
CGAATGGACA GTCAGAGCGA AGCGGTCGCC ATCCAAGCCA TTCGGAATGC TAAAGGGAAT 
TCATTTTGTG TGGATTGTGG TGCCCCGAAT CCCACCTGGG CCAGCTTGAA TCTGGGTGCC 
CTGATCTGCA TTGAATGTTC AGGAATTCAC AGAAACCTTG GCACCCATTT ATCTCGGGTC 
CGATCACTGG ATTTGGATGA CTGGCCCCTA GAGCTCACAC TTGTTCTAAC CTCTATTGGT 
AATGAAATGG CCAACAGCAT ATGGGAGATG ACAACTCATG GAAGAACCAA ACCAGCACCT 
GATTCCTCAA GGGAAGAGAG AGAATCATGG ATTAGAGCTA AGTATGAGCA GCGATTATTT 
TTGGCCCCTT TGAAGAGTCC AGGGGTCCCC TTGGTGAAGC AGCTTTTTAA AGCTGTACAT 
GAGAAAAATC TCGGCAATGT ACTTCTCCTC CTAGCCCACA GCAACAAGGA GCAAATCAAC 
GGAATCACTG GAGACAGAGA CAGACGCACT GCCCTTCATG TGGCCTGTGA ACTGGGGGAA 
GTTGTGACCA CACAACTGCT TGTCTGGTAT GGCATCGATG TGAAGTCAAG AGACAGCAAT 
GGCCACACAG CCGTGTTTTA TGCTAAGAAA AGTAACAGCC AAGAGTGCGT GGACATTCTG 
CTGCAACATG GCTGTCCCAA TGAGTCCACA AGCGCCCTGT CCACTCCCAG TCTAAGAAGG 
AAAAGCAGCT CTGCCAGCAT CGTGAGGACT GATTCCCGGA CTGCCCTTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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agap2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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