dgkk gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004916780.3	XP_004916837.1	diacylglycerol kinase kappa isoform X2
XM_002939213.4	XP_002939259.2	diacylglycerol kinase kappa isoform X4
XM_031891294.1	XP_031747154.1	diacylglycerol kinase kappa isoform X5
XM_018096866.1	XP_017952355.1	diacylglycerol kinase kappa isoform X5
XM_004916780.4	XP_004916837.1	diacylglycerol kinase kappa isoform X2
XM_002939213.3	XP_002939259.2	diacylglycerol kinase kappa isoform X4
XM_004916779.4	XP_004916836.1	diacylglycerol kinase kappa isoform X1
XM_004916779.3	XP_004916836.1	diacylglycerol kinase kappa isoform X1
XM_004916781.3	XP_004916838.1	diacylglycerol kinase kappa isoform X3
XM_004916781.4	XP_004916838.1	diacylglycerol kinase kappa isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa46467	XM_018096866.1 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa64558	XM_004916779.4 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64559	XM_004916780.4 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64560	XM_004916781.4 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64561	XM_002939213.4 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa64562	XM_031891294.1 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa14246	XM_004916779.3 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa14247	XM_004916780.3 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa14248	XM_004916781.3 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa14249	XM_002939213.3 Latest version!	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

dgkk ( XM_018096866.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952355.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa46467
Clone ID Related Accession (Same CDS sequence)	XM_018096866.1
Accession Version	XM_018096866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT GGATTGGAGC CCTGAAATCA GTACAGAAAT GGGAGCTCAA TGAGACAACC 
CAGTTTAATA TGGAACACTT TTCTGGAATG CACAACTGGT ACGCTTGTTC CCATGCTCGT 
CCTACCTTCT GCAATGTGTG CAGAGAGGCA CTGTCTGGAG TCACATCCCA TGGGCTATCC 
TGTGAAGTCT GCAAGTTTAA AGCTCACAAG CGGTGCGCCG TAAGAGCCAC AAATAACTGC 
AAATGGACAA CACTAGCATC TGTGGGAAAT GACATCATAG AAGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGCTAGA AGGCAACCTG CCTGTCAGTG CCAAATGTGA AGTGTGTGAC 
AAGACATGTG GAAGTGTGCG GAGGCTGCAG GACTGGAGGT GCCTGTGGTG TAAAGCTATA 
GTGCACACAT CCTGTAAGGA GCAGCTGGGC AAAATCTGCC CTCTTGGACC ATGCAGAGTC 
TCTATTATCC CTCCTATTGC TCTTAATAGC ATTGACTCTG ATGGGTTCTG GAAGGCAACC 
TCACCTGCCT CGTGCGCCAG CCCCCTACTC GTCTTTGTGA ATTCTAAAAG CGGAGACAAC 
CAGGGTGTGA AATTTCTTCG AAAGTTCAAG CAGTTCCTTA ACCCCGCGCA GGTCTTTGAT 
CTCATGAATG GGGGGCCACA GCTGGGACTG CGCTTGTTCC AGAAATTCTC AAACTTTCGT 
ATACTGGTGT GTGGCGGAGA CGGCAGCGTT GGGTGGGTCT TGTCAGAGAT TGACAAGTTA 
GGACTGCAGA AACAGTGCCA GCTAGGAGTT CTGCCACTGG GCACAGGGAA TGACTTGGCA 
CGGGTGCTGG GTTGGGGAAG TCTATGTGAT GATGATGCTC AGCTACTCCA GATACTAGAG 
AAGCTGGAAC GTGCCACCAC CAAAATGCTT GACAGATGGA GCATATTAAC ATATGAATCC 
CCAAAGCACT CTCCCCCTGC CATCAGAGAG GAGGAGAATG GAGAGGCCAA ATTTCAGGTA 
CAGATATCCC AGTATGCCGA TTCTGTTGCC TTCCACCTGG CTAAAATTCT GGAATCAGAT 
AAGCACTGCG ATGTCATTTC ATCTGCCAAG TTCCTGTGCC TTTCAGTAAA TGATTTCATC 
ACTGAAGTTG GGAAGGCGTA CGCAAGGGCA GCAGAAAACC GTGAAGAGGC AGACAACATG 
GCTAGAAAGT GCTCAATGTT AAATGAGAAG CTGGAGTCTC TGGTTCGGTC CCTTAATGAA 
GAGTCACAAG CATTACTAGC TCCTCCCTTA CAGAGCCTTC ACCCTGACTC TGCCCTGAAG 
GAAAATAGCA AGGATGTGAA TGAAAAGGAA AATAACATGG AGGACATGGC TAAAATATTC 
AAATCTAAGG AGCAGCTCAT GCTAAGGGCC AATAGTCTAA AGAAAGCTCT GAAACAGATC 
ATAGAGCAGG CAGAGAAAGC TGTGGATGAA CAGAACAAAC ACACACAAGT TTACAGCACC 
CCCATGTTAT CCCCCATTCA GAAGGAGAGC TCTGAAGAAT TCAGCAAAGA AGGAGAGAGT 
CTGGCTTCCA GGCGTGGAAC TATCTCATCT ACCACATCCT CTATCATTCT GGACCGGCCG 
TATACCTTCA GTCCCCTGCA CTTTAGCGAG GATGCTATCC AGTTCTCAGA AAGATGCACT 
ATGAATAATT ATTTTGGCAT TGGCCTGGAT GCCAAGATAT CGCTAGAGTT TAATAACAAA 
CGAGATGAGC ACCCCAAGAA GTGCAGCAGT CGGACCCGGA ACATGATGTG GTATGGTGTT 
CTGGGCACCA AGGAACTTCT TCAGAAAACC TACAAGAATT TGGAGCAGCG TGTCAAGCTT 
GAGTGTGACG GGGAACCCAT CTCTCTGCCA AGCCTTCAGG GTATTGCTGT TCTGAACATC 
CCCAGCTACG CTGGTGGCAT AAACTTCTGG GGAGGAACCA AAGAGGACAA TAATTTTGGG 
GCTCCATCTT TTGACGACAA AAAGCTAGAG GTGGTGGCTG TGTTTGGGAG CATCCAGATG 
GCTGTGTCAC GTGTAATCAA TCTGCAGCAC CATCGTATTG CTCAGTGCCG GGTAGTGAAG 
ATCACGATCC TCGGAGAAGA AGGTGTACCT GTGCAGGTGG ATGGCGAGGC TTGGGTGCAG 
CCTCCTGGGA TCCTGAAAAT CCAACACAAG AACCGGGCAC AGATGCTCAC TAGAGACAGG 
GCTTTTGAGA ACACTCTGAA ATCCTGGGAA GACAAGAGAA AGGGAGACAG TTACAGGGTT 
ATGGCACGGC CGAGGCTTCA CTCCCAGCAG TCAATGGAGT ACCTTACAGA GGAAGAGTTT 
GCACATCTGC AGCACTTGGC TAACACTTCT GAGAATCTCA TCAGCCGGAT TCGGGAAGCT 
GCCAAGTATC ACAAGGCTGT AGAGCAGGAG TTGGCCCATG CTGTCAATGG AAGCTTTGGG 
GTGCTCAATG AAGCATTTTC CAACAAAACC AACAACTCTC CCGAGTTGCT GAGCAGAAAT 
ACGGCTGTGG ATGTTGCCAT AAGTGTGAAG GCATTATATA CAGAGACAAA GGCTTTCCTG 
GAGAGCAAGC AGCAATGGGA ATCTCCCCAG GAGGAGGAAG GGCTTCTCTG TTCCCTGAAT 
GCAGTGGGCC AAGACTTGGA GAGACTGGCT GATATCCACT GGATCTCACA CATCATCAAA 
CCAACTGAGG AGGAGAGCAG TGGAGGAGGC AACAAGGGGA GCTTTAAACT GCGAATCATT 
CCAAAACCAA AAAAGGAAAA GGAGAAGTGT CAGAAGCAGA AATCCAACAG CTCTCTAACA 
GTGGATAAAT GGGGCACAGA GGAGGTGGCT GCGTGGCTGG ACGTTTTGGG TTTAGGGGAA 
TACAAGGATA TATTTATCCG CCATGACATC CAAGGCTCAG AGCTGGTATT ACTGGAAAGG 
AGAGACCTCA AGGACCTGGG AATAGCGAAA GTTGGTCATA TGAAGAGAAT TCTACAGGGT 
ATTAAGGATA TAACTACCCC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952355.1
CDS	465..3491
Translation	MEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_018096866.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096866.1

ORF Insert Sequence:

ATGGAGGACT GGATTGGAGC CCTGAAATCA GTACAGAAAT GGGAGCTCAA TGAGACAACC 
CAGTTTAATA TGGAACACTT TTCTGGAATG CACAACTGGT ACGCTTGTTC CCATGCTCGT 
CCTACCTTCT GCAATGTGTG CAGAGAGGCA CTGTCTGGAG TCACATCCCA TGGGCTATCC 
TGTGAAGTCT GCAAGTTTAA AGCTCACAAG CGGTGCGCCG TAAGAGCCAC AAATAACTGC 
AAATGGACAA CACTAGCATC TGTGGGAAAT GACATCATAG AAGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGCTAGA AGGCAACCTG CCTGTCAGTG CCAAATGTGA AGTGTGTGAC 
AAGACATGTG GAAGTGTGCG GAGGCTGCAG GACTGGAGGT GCCTGTGGTG TAAAGCTATA 
GTGCACACAT CCTGTAAGGA GCAGCTGGGC AAAATCTGCC CTCTTGGACC ATGCAGAGTC 
TCTATTATCC CTCCTATTGC TCTTAATAGC ATTGACTCTG ATGGGTTCTG GAAGGCAACC 
TCACCTGCCT CGTGCGCCAG CCCCCTACTC GTCTTTGTGA ATTCTAAAAG CGGAGACAAC 
CAGGGTGTGA AATTTCTTCG AAAGTTCAAG CAGTTCCTTA ACCCCGCGCA GGTCTTTGAT 
CTCATGAATG GGGGGCCACA GCTGGGACTG CGCTTGTTCC AGAAATTCTC AAACTTTCGT 
ATACTGGTGT GTGGCGGAGA CGGCAGCGTT GGGTGGGTCT TGTCAGAGAT TGACAAGTTA 
GGACTGCAGA AACAGTGCCA GCTAGGAGTT CTGCCACTGG GCACAGGGAA TGACTTGGCA 
CGGGTGCTGG GTTGGGGAAG TCTATGTGAT GATGATGCTC AGCTACTCCA GATACTAGAG 
AAGCTGGAAC GTGCCACCAC CAAAATGCTT GACAGATGGA GCATATTAAC ATATGAATCC 
CCAAAGCACT CTCCCCCTGC CATCAGAGAG GAGGAGAATG GAGAGGCCAA ATTTCAGGTA 
CAGATATCCC AGTATGCCGA TTCTGTTGCC TTCCACCTGG CTAAAATTCT GGAATCAGAT 
AAGCACTGCG ATGTCATTTC ATCTGCCAAG TTCCTGTGCC TTTCAGTAAA TGATTTCATC 
ACTGAAGTTG GGAAGGCGTA CGCAAGGGCA GCAGAAAACC GTGAAGAGGC AGACAACATG 
GCTAGAAAGT GCTCAATGTT AAATGAGAAG CTGGAGTCTC TGGTTCGGTC CCTTAATGAA 
GAGTCACAAG CATTACTAGC TCCTCCCTTA CAGAGCCTTC ACCCTGACTC TGCCCTGAAG 
GAAAATAGCA AGGATGTGAA TGAAAAGGAA AATAACATGG AGGACATGGC TAAAATATTC 
AAATCTAAGG AGCAGCTCAT GCTAAGGGCC AATAGTCTAA AGAAAGCTCT GAAACAGATC 
ATAGAGCAGG CAGAGAAAGC TGTGGATGAA CAGAACAAAC ACACACAAGT TTACAGCACC 
CCCATGTTAT CCCCCATTCA GAAGGAGAGC TCTGAAGAAT TCAGCAAAGA AGGAGAGAGT 
CTGGCTTCCA GGCGTGGAAC TATCTCATCT ACCACATCCT CTATCATTCT GGACCGGCCG 
TATACCTTCA GTCCCCTGCA CTTTAGCGAG GATGCTATCC AGTTCTCAGA AAGATGCACT 
ATGAATAATT ATTTTGGCAT TGGCCTGGAT GCCAAGATAT CGCTAGAGTT TAATAACAAA 
CGAGATGAGC ACCCCAAGAA GTGCAGCAGT CGGACCCGGA ACATGATGTG GTATGGTGTT 
CTGGGCACCA AGGAACTTCT TCAGAAAACC TACAAGAATT TGGAGCAGCG TGTCAAGCTT 
GAGTGTGACG GGGAACCCAT CTCTCTGCCA AGCCTTCAGG GTATTGCTGT TCTGAACATC 
CCCAGCTACG CTGGTGGCAT AAACTTCTGG GGAGGAACCA AAGAGGACAA TAATTTTGGG 
GCTCCATCTT TTGACGACAA AAAGCTAGAG GTGGTGGCTG TGTTTGGGAG CATCCAGATG 
GCTGTGTCAC GTGTAATCAA TCTGCAGCAC CATCGTATTG CTCAGTGCCG GGTAGTGAAG 
ATCACGATCC TCGGAGAAGA AGGTGTACCT GTGCAGGTGG ATGGCGAGGC TTGGGTGCAG 
CCTCCTGGGA TCCTGAAAAT CCAACACAAG AACCGGGCAC AGATGCTCAC TAGAGACAGG 
GCTTTTGAGA ACACTCTGAA ATCCTGGGAA GACAAGAGAA AGGGAGACAG TTACAGGGTT 
ATGGCACGGC CGAGGCTTCA CTCCCAGCAG TCAATGGAGT ACCTTACAGA GGAAGAGTTT 
GCACATCTGC AGCACTTGGC TAACACTTCT GAGAATCTCA TCAGCCGGAT TCGGGAAGCT 
GCCAAGTATC ACAAGGCTGT AGAGCAGGAG TTGGCCCATG CTGTCAATGG AAGCTTTGGG 
GTGCTCAATG AAGCATTTTC CAACAAAACC AACAACTCTC CCGAGTTGCT GAGCAGAAAT 
ACGGCTGTGG ATGTTGCCAT AAGTGTGAAG GCATTATATA CAGAGACAAA GGCTTTCCTG 
GAGAGCAAGC AGCAATGGGA ATCTCCCCAG GAGGAGGAAG GGCTTCTCTG TTCCCTGAAT 
GCAGTGGGCC AAGACTTGGA GAGACTGGCT GATATCCACT GGATCTCACA CATCATCAAA 
CCAACTGAGG AGGAGAGCAG TGGAGGAGGC AACAAGGGGA GCTTTAAACT GCGAATCATT 
CCAAAACCAA AAAAGGAAAA GGAGAAGTGT CAGAAGCAGA AATCCAACAG CTCTCTAACA 
GTGGATAAAT GGGGCACAGA GGAGGTGGCT GCGTGGCTGG ACGTTTTGGG TTTAGGGGAA 
TACAAGGATA TATTTATCCG CCATGACATC CAAGGCTCAG AGCTGGTATT ACTGGAAAGG 
AGAGACCTCA AGGACCTGGG AATAGCGAAA GTTGGTCATA TGAAGAGAAT TCTACAGGGT 
ATTAAGGATA TAACTACCCC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916779.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916836.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa64558
Clone ID Related Accession (Same CDS sequence)	XM_004916779.4
Accession Version	XM_004916779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGTGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGT GGATAAATGG GGCACAGAGG AGGTGGCTGC GTGGCTGGAC 
GTTTTGGGTT TAGGGGAATA CAAGGATATA TTTATCCGCC ATGACATCCA AGGCTCAGAG 
CTGGTATTAC TGGAAAGGAG AGACCTCAAG GACCTGGGAA TAGCGAAAGT TGGTCATATG 
AAGAGAATTC TACAGGGTAT TAAGGATATA ACTACCCCTA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916836.1
CDS	349..3813
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916779.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916779.4

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGTGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGT GGATAAATGG GGCACAGAGG AGGTGGCTGC GTGGCTGGAC 
GTTTTGGGTT TAGGGGAATA CAAGGATATA TTTATCCGCC ATGACATCCA AGGCTCAGAG 
CTGGTATTAC TGGAAAGGAG AGACCTCAAG GACCTGGGAA TAGCGAAAGT TGGTCATATG 
AAGAGAATTC TACAGGGTAT TAAGGATATA ACTACCCCTA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916780.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916837.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.

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CloneID	OXa64559
Clone ID Related Accession (Same CDS sequence)	XM_004916780.4
Accession Version	XM_004916780.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916780.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGATATC CCAGTATGCC GATTCTGTTG CCTTCCACCT GGCTAAAATT 
CTGGAATCAG ATAAGCACTG TGATGTCATT TCATCTGCCA AGTTCCTGTG CCTTTCAGTA 
AATGATTTCA TCACTGAAGT TGGGAAGGCG TACGCAAGGG CAGCAGAAAA CCGTGAAGAG 
GCAGACAACA TGGCTAGAAA GTGCTCAATG TTAAATGAGA AGCTGGAGTC TCTGGTTCGG 
TCCCTTAATG AAGAGTCACA AGCATTACTA GCTCCTCCCT TACAGAGCCT TCACCCTGAC 
TCTGCCCTGA AGGAAAATAG CAAGGATGTG AATGAAAAGG AAAATAACAT GGAGGACATG 
GCTAAAATAT TCAAATCTAA GGAGCAGCTC ATGCTAAGGG CCAATAGTCT AAAGAAAGCT 
CTGAAACAGA TCATAGAGCA GGCAGAGAAA GCTGTGGATG AACAGAACAA ACACACACAA 
GTTTACAGCA CCCCCATGTT ATCCCCCATT CAGAAGGAGA GCTCTGAAGA ATTCAGCAAA 
GAAGGAGAGA GTCTGGCTTC CAGGCGTGGA ACTATCTCAT CTACCACATC CTCTATCATT 
CTGGACCGGC CGTATACCTT CAGTCCCCTG CACTTTAGCG AGGATGCTAT CCAGTTCTCA 
GAAAGATGCA CTATGAATAA TTATTTTGGC ATTGGCCTGG ATGCCAAGAT ATCGCTAGAG 
TTTAATAACA AACGAGATGA GCACCCCAAG AAGTGCAGCA GTCGGACCCG GAACATGATG 
TGGTATGGTG TTCTGGGCAC CAAGGAACTT CTTCAGAAAA CCTACAAGAA TTTGGAGCAG 
CGTGTCAAGC TTGAGTGTGA CGGGGAACCC ATCTCTCTGC CAAGCCTTCA GGGTATTGCT 
GTTCTGAACA TCCCCAGCTA CGCTGGTGGC ATAAACTTCT GGGGAGGAAC CAAAGAGGAC 
AATAATTTTG GGGCTCCATC TTTTGACGAC AAAAAGCTAG AGGTGGTGGC TGTGTTTGGG 
AGCATCCAGA TGGCTGTGTC ACGTGTAATC AATCTGCAGC ACCATCGTAT TGCTCAGTGC 
CGGGTAGTGA AGATCACGAT CCTCGGAGAA GAAGGTGTAC CTGTGCAGGT GGATGGCGAG 
GCTTGGGTGC AGCCTCCTGG GATCCTGAAA ATCCAACACA AGAACCGGGC ACAGATGCTC 
ACTAGAGACA GGGCTTTTGA GAACACTCTG AAATCCTGGG AAGACAAGAG AAAGGGAGAC 
AGTTACAGGG TTATGGCACG GCCGAGGCTT CACTCCCAGC AGTCAATGGA GTACCTTACA 
GAGGAAGAGT TTGCACATCT GCAGCACTTG GCTAACACTT CTGAGAATCT CATCAGCCGG 
ATTCGGGAAG CTGCCAAGTA TCACAAGGCT GTAGAGCAGG AGTTGGCCCA TGCTGTCAAT 
GGAAGCTTTG GGGTGCTCAA TGAAGCATTT TCCAACAAAA CCAACAACTC TCCCGAGTTG 
CTGAGCAGAA ATACGGCTGT GGATGTTGCC ATAAGTGTGA AGGCATTATA TACAGAGACA 
AAGGCTTTCC TGGAGAGCAA GCAGCAATGG GAATCTCCCC AGGAGGAGGA AGGGCTTCTC 
TGTTCCCTGA ATGCAGTGGG CCAAGACTTG GAGAGACTGG CTGATATCCA CTGGATCTCA 
CACATCATCA AACCAACTGA GGAGGAGAGC AGTGGAGGAG GCAACAAGGG GAGCTTTAAA 
CTGCGAATCA TTCCAAAACC AAAAAAGGAA AAGGAGAAGT GTCAGAAGCA GAAATCCAAC 
AGCTCTCTAA CAGTGGATAA ATGGGGCACA GAGGAGGTGG CTGCGTGGCT GGACGTTTTG 
GGTTTAGGGG AATACAAGGA TATATTTATC CGCCATGACA TCCAAGGCTC AGAGCTGGTA 
TTACTGGAAA GGAGAGACCT CAAGGACCTG GGAATAGCGA AAGTTGGTCA TATGAAGAGA 
ATTCTACAGG GTATTAAGGA TATAACTACC CCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916837.1
CDS	349..3807
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916780.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916780.4

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGATATC CCAGTATGCC GATTCTGTTG CCTTCCACCT GGCTAAAATT 
CTGGAATCAG ATAAGCACTG TGATGTCATT TCATCTGCCA AGTTCCTGTG CCTTTCAGTA 
AATGATTTCA TCACTGAAGT TGGGAAGGCG TACGCAAGGG CAGCAGAAAA CCGTGAAGAG 
GCAGACAACA TGGCTAGAAA GTGCTCAATG TTAAATGAGA AGCTGGAGTC TCTGGTTCGG 
TCCCTTAATG AAGAGTCACA AGCATTACTA GCTCCTCCCT TACAGAGCCT TCACCCTGAC 
TCTGCCCTGA AGGAAAATAG CAAGGATGTG AATGAAAAGG AAAATAACAT GGAGGACATG 
GCTAAAATAT TCAAATCTAA GGAGCAGCTC ATGCTAAGGG CCAATAGTCT AAAGAAAGCT 
CTGAAACAGA TCATAGAGCA GGCAGAGAAA GCTGTGGATG AACAGAACAA ACACACACAA 
GTTTACAGCA CCCCCATGTT ATCCCCCATT CAGAAGGAGA GCTCTGAAGA ATTCAGCAAA 
GAAGGAGAGA GTCTGGCTTC CAGGCGTGGA ACTATCTCAT CTACCACATC CTCTATCATT 
CTGGACCGGC CGTATACCTT CAGTCCCCTG CACTTTAGCG AGGATGCTAT CCAGTTCTCA 
GAAAGATGCA CTATGAATAA TTATTTTGGC ATTGGCCTGG ATGCCAAGAT ATCGCTAGAG 
TTTAATAACA AACGAGATGA GCACCCCAAG AAGTGCAGCA GTCGGACCCG GAACATGATG 
TGGTATGGTG TTCTGGGCAC CAAGGAACTT CTTCAGAAAA CCTACAAGAA TTTGGAGCAG 
CGTGTCAAGC TTGAGTGTGA CGGGGAACCC ATCTCTCTGC CAAGCCTTCA GGGTATTGCT 
GTTCTGAACA TCCCCAGCTA CGCTGGTGGC ATAAACTTCT GGGGAGGAAC CAAAGAGGAC 
AATAATTTTG GGGCTCCATC TTTTGACGAC AAAAAGCTAG AGGTGGTGGC TGTGTTTGGG 
AGCATCCAGA TGGCTGTGTC ACGTGTAATC AATCTGCAGC ACCATCGTAT TGCTCAGTGC 
CGGGTAGTGA AGATCACGAT CCTCGGAGAA GAAGGTGTAC CTGTGCAGGT GGATGGCGAG 
GCTTGGGTGC AGCCTCCTGG GATCCTGAAA ATCCAACACA AGAACCGGGC ACAGATGCTC 
ACTAGAGACA GGGCTTTTGA GAACACTCTG AAATCCTGGG AAGACAAGAG AAAGGGAGAC 
AGTTACAGGG TTATGGCACG GCCGAGGCTT CACTCCCAGC AGTCAATGGA GTACCTTACA 
GAGGAAGAGT TTGCACATCT GCAGCACTTG GCTAACACTT CTGAGAATCT CATCAGCCGG 
ATTCGGGAAG CTGCCAAGTA TCACAAGGCT GTAGAGCAGG AGTTGGCCCA TGCTGTCAAT 
GGAAGCTTTG GGGTGCTCAA TGAAGCATTT TCCAACAAAA CCAACAACTC TCCCGAGTTG 
CTGAGCAGAA ATACGGCTGT GGATGTTGCC ATAAGTGTGA AGGCATTATA TACAGAGACA 
AAGGCTTTCC TGGAGAGCAA GCAGCAATGG GAATCTCCCC AGGAGGAGGA AGGGCTTCTC 
TGTTCCCTGA ATGCAGTGGG CCAAGACTTG GAGAGACTGG CTGATATCCA CTGGATCTCA 
CACATCATCA AACCAACTGA GGAGGAGAGC AGTGGAGGAG GCAACAAGGG GAGCTTTAAA 
CTGCGAATCA TTCCAAAACC AAAAAAGGAA AAGGAGAAGT GTCAGAAGCA GAAATCCAAC 
AGCTCTCTAA CAGTGGATAA ATGGGGCACA GAGGAGGTGG CTGCGTGGCT GGACGTTTTG 
GGTTTAGGGG AATACAAGGA TATATTTATC CGCCATGACA TCCAAGGCTC AGAGCTGGTA 
TTACTGGAAA GGAGAGACCT CAAGGACCTG GGAATAGCGA AAGTTGGTCA TATGAAGAGA 
ATTCTACAGG GTATTAAGGA TATAACTACC CCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916781.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916838.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa64560
Clone ID Related Accession (Same CDS sequence)	XM_004916781.4
Accession Version	XM_004916781.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTTA TTTCAGACAC ATATCAACTT GGAGTAGGGC ATTCTTTTAC AACCTCTGTG 
CATGAAGAAG CCCCCAGGTT CTGGTGGAGT CTGGCGTGGC AGAACCCTAT CTTTATTATG 
TTGGAGAGAA CCCAAGATCA TCCATTTTTT CAGAAGAGCG TGAAAGAGGG AATCCTGCTA 
AAACAGACCA GCTCATTCCA GAGGTGGAAA AGGCGATATT TTAAACTGCG CGGAAGAACA 
CTTTACTATG CTAAGGATTC AAAGTCTCTC ATTTTTGATG AAGTTGATCT CTCAGATGCT 
AGTGTGGCTG AAACCAGTAC CAAGAATATT AATAACAGCT TCACGGTCAT CACCCCATTC 
CGCAAGCTTA TCCTGTGTGC TGAGAACAGA AAGGAGATGG AGGACTGGAT TGGAGCCCTG 
AAATCAGTAC AGAAATGGGA GCTCAATGAG ACAACCCAGT TTAATATGGA ACACTTTTCT 
GGAATGCACA ACTGGTACGC TTGTTCCCAT GCTCGTCCTA CCTTCTGCAA TGTGTGCAGA 
GAGGCACTGT CTGGAGTCAC ATCCCATGGG CTATCCTGTG AAGTCTGCAA GTTTAAAGCT 
CACAAGCGGT GCGCCGTAAG AGCCACAAAT AACTGCAAAT GGACAACACT AGCATCTGTG 
GGAAATGACA TCATAGAAGA TGAGGATGGA GTGTCAATGC CTCACCAGTG GCTAGAAGGC 
AACCTGCCTG TCAGTGCCAA ATGTGAAGTG TGTGACAAGA CATGTGGAAG TGTGCGGAGG 
CTGCAGGACT GGAGGTGCCT GTGGTGTAAA GCTATAGTGC ACACATCCTG TAAGGAGCAG 
CTGGGCAAAA TCTGCCCTCT TGGACCATGC AGAGTCTCTA TTATCCCTCC TATTGCTCTT 
AATAGCATTG ACTCTGATGG GTTCTGGAAG GCAACCTCAC CTGCCTCGTG CGCCAGCCCC 
CTACTCGTCT TTGTGAATTC TAAAAGCGGA GACAACCAGG GTGTGAAATT TCTTCGAAAG 
TTCAAGCAGT TCCTTAACCC CGCGCAGGTC TTTGATCTCA TGAATGGGGG GCCACAGCTG 
GGACTGCGCT TGTTCCAGAA ATTCTCAAAC TTTCGTATAC TGGTGTGTGG CGGAGACGGC 
AGCGTTGGGT GGGTCTTGTC AGAGATTGAC AAGTTAGGAC TGCAGAAACA GTGCCAGCTA 
GGAGTTCTGC CACTGGGCAC AGGGAATGAC TTGGCACGGG TGCTGGGTTG GGGAAGTCTA 
TGTGATGATG ATGCTCAGCT ACTCCAGATA CTAGAGAAGC TGGAACGTGC CACCACCAAA 
ATGCTTGACA GATGGAGCAT ATTAACATAT GAATCCCCAA AGCACTCTCC CCCTGCCATC 
AGAGAGGAGG AGAATGGAGA GGCCAAATTT CAGGTACAGA TATCCCAGTA TGCCGATTCT 
GTTGCCTTCC ACCTGGCTAA AATTCTGGAA TCAGATAAGC ACTGTGATGT CATTTCATCT 
GCCAAGTTCC TGTGCCTTTC AGTAAATGAT TTCATCACTG AAGTTGGGAA GGCGTACGCA 
AGGGCAGCAG AAAACCGTGA AGAGGCAGAC AACATGGCTA GAAAGTGCTC AATGTTAAAT 
GAGAAGCTGG AGTCTCTGGT TCGGTCCCTT AATGAAGAGT CACAAGCATT ACTAGCTCCT 
CCCTTACAGA GCCTTCACCC TGACTCTGCC CTGAAGGAAA ATAGCAAGGA TGTGAATGAA 
AAGGAAAATA ACATGGAGGA CATGGCTAAA ATATTCAAAT CTAAGGAGCA GCTCATGCTA 
AGGGCCAATA GTCTAAAGAA AGCTCTGAAA CAGATCATAG AGCAGGCAGA GAAAGCTGTG 
GATGAACAGA ACAAACACAC ACAAGTTTAC AGCACCCCCA TGTTATCCCC CATTCAGAAG 
GAGAGCTCTG AAGAATTCAG CAAAGAAGGA GAGAGTCTGG CTTCCAGGCG TGGAACTATC 
TCATCTACCA CATCCTCTAT CATTCTGGAC CGGCCGTATA CCTTCAGTCC CCTGCACTTT 
AGCGAGGATG CTATCCAGTT CTCAGAAAGA TGCACTATGA ATAATTATTT TGGCATTGGC 
CTGGATGCCA AGATATCGCT AGAGTTTAAT AACAAACGAG ATGAGCACCC CAAGAAGTGC 
AGCAGTCGGA CCCGGAACAT GATGTGGTAT GGTGTTCTGG GCACCAAGGA ACTTCTTCAG 
AAAACCTACA AGAATTTGGA GCAGCGTGTC AAGCTTGAGT GTGACGGGGA ACCCATCTCT 
CTGCCAAGCC TTCAGGGTAT TGCTGTTCTG AACATCCCCA GCTACGCTGG TGGCATAAAC 
TTCTGGGGAG GAACCAAAGA GGACAATAAT TTTGGGGCTC CATCTTTTGA CGACAAAAAG 
CTAGAGGTGG TGGCTGTGTT TGGGAGCATC CAGATGGCTG TGTCACGTGT AATCAATCTG 
CAGCACCATC GTATTGCTCA GTGCCGGGTA GTGAAGATCA CGATCCTCGG AGAAGAAGGT 
GTACCTGTGC AGGTGGATGG CGAGGCTTGG GTGCAGCCTC CTGGGATCCT GAAAATCCAA 
CACAAGAACC GGGCACAGAT GCTCACTAGA GACAGGGCTT TTGAGAACAC TCTGAAATCC 
TGGGAAGACA AGAGAAAGGG AGACAGTTAC AGGGTTATGG CACGGCCGAG GCTTCACTCC 
CAGCAGTCAA TGGAGTACCT TACAGAGGAA GAGTTTGCAC ATCTGCAGCA CTTGGCTAAC 
ACTTCTGAGA ATCTCATCAG CCGGATTCGG GAAGCTGCCA AGTATCACAA GGCTGTAGAG 
CAGGAGTTGG CCCATGCTGT CAATGGAAGC TTTGGGGTGC TCAATGAAGC ATTTTCCAAC 
AAAACCAACA ACTCTCCCGA GTTGCTGAGC AGAAATACGG CTGTGGATGT TGCCATAAGT 
GTGAAGGCAT TATATACAGA GACAAAGGCT TTCCTGGAGA GCAAGCAGCA ATGGGAATCT 
CCCCAGGAGG AGGAAGGGCT TCTCTGTTCC CTGAATGCAG TGGGCCAAGA CTTGGAGAGA 
CTGGCTGATA TCCACTGGAT CTCACACATC ATCAAACCAA CTGAGGAGGA GAGCAGTGGA 
GGAGGCAACA AGGGGAGCTT TAAACTGCGA ATCATTCCAA AACCAAAAAA GGAAAAGGAG 
AAGTGTCAGA AGCAGAAATC CAACAGCTCT CTAACAGTGG ATAAATGGGG CACAGAGGAG 
GTGGCTGCGT GGCTGGACGT TTTGGGTTTA GGGGAATACA AGGATATATT TATCCGCCAT 
GACATCCAAG GCTCAGAGCT GGTATTACTG GAAAGGAGAG ACCTCAAGGA CCTGGGAATA 
GCGAAAGTTG GTCATATGAA GAGAATTCTA CAGGGTATTA AGGATATAAC TACCCCTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916838.1
CDS	271..3693
Translation	MVLISDTYQLGVGHSFTTSVHEEAPRFWWSLAWQNPIFIMLERTQDHPFFQKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916781.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916781.4

ORF Insert Sequence:

ATGGTCCTTA TTTCAGACAC ATATCAACTT GGAGTAGGGC ATTCTTTTAC AACCTCTGTG 
CATGAAGAAG CCCCCAGGTT CTGGTGGAGT CTGGCGTGGC AGAACCCTAT CTTTATTATG 
TTGGAGAGAA CCCAAGATCA TCCATTTTTT CAGAAGAGCG TGAAAGAGGG AATCCTGCTA 
AAACAGACCA GCTCATTCCA GAGGTGGAAA AGGCGATATT TTAAACTGCG CGGAAGAACA 
CTTTACTATG CTAAGGATTC AAAGTCTCTC ATTTTTGATG AAGTTGATCT CTCAGATGCT 
AGTGTGGCTG AAACCAGTAC CAAGAATATT AATAACAGCT TCACGGTCAT CACCCCATTC 
CGCAAGCTTA TCCTGTGTGC TGAGAACAGA AAGGAGATGG AGGACTGGAT TGGAGCCCTG 
AAATCAGTAC AGAAATGGGA GCTCAATGAG ACAACCCAGT TTAATATGGA ACACTTTTCT 
GGAATGCACA ACTGGTACGC TTGTTCCCAT GCTCGTCCTA CCTTCTGCAA TGTGTGCAGA 
GAGGCACTGT CTGGAGTCAC ATCCCATGGG CTATCCTGTG AAGTCTGCAA GTTTAAAGCT 
CACAAGCGGT GCGCCGTAAG AGCCACAAAT AACTGCAAAT GGACAACACT AGCATCTGTG 
GGAAATGACA TCATAGAAGA TGAGGATGGA GTGTCAATGC CTCACCAGTG GCTAGAAGGC 
AACCTGCCTG TCAGTGCCAA ATGTGAAGTG TGTGACAAGA CATGTGGAAG TGTGCGGAGG 
CTGCAGGACT GGAGGTGCCT GTGGTGTAAA GCTATAGTGC ACACATCCTG TAAGGAGCAG 
CTGGGCAAAA TCTGCCCTCT TGGACCATGC AGAGTCTCTA TTATCCCTCC TATTGCTCTT 
AATAGCATTG ACTCTGATGG GTTCTGGAAG GCAACCTCAC CTGCCTCGTG CGCCAGCCCC 
CTACTCGTCT TTGTGAATTC TAAAAGCGGA GACAACCAGG GTGTGAAATT TCTTCGAAAG 
TTCAAGCAGT TCCTTAACCC CGCGCAGGTC TTTGATCTCA TGAATGGGGG GCCACAGCTG 
GGACTGCGCT TGTTCCAGAA ATTCTCAAAC TTTCGTATAC TGGTGTGTGG CGGAGACGGC 
AGCGTTGGGT GGGTCTTGTC AGAGATTGAC AAGTTAGGAC TGCAGAAACA GTGCCAGCTA 
GGAGTTCTGC CACTGGGCAC AGGGAATGAC TTGGCACGGG TGCTGGGTTG GGGAAGTCTA 
TGTGATGATG ATGCTCAGCT ACTCCAGATA CTAGAGAAGC TGGAACGTGC CACCACCAAA 
ATGCTTGACA GATGGAGCAT ATTAACATAT GAATCCCCAA AGCACTCTCC CCCTGCCATC 
AGAGAGGAGG AGAATGGAGA GGCCAAATTT CAGGTACAGA TATCCCAGTA TGCCGATTCT 
GTTGCCTTCC ACCTGGCTAA AATTCTGGAA TCAGATAAGC ACTGTGATGT CATTTCATCT 
GCCAAGTTCC TGTGCCTTTC AGTAAATGAT TTCATCACTG AAGTTGGGAA GGCGTACGCA 
AGGGCAGCAG AAAACCGTGA AGAGGCAGAC AACATGGCTA GAAAGTGCTC AATGTTAAAT 
GAGAAGCTGG AGTCTCTGGT TCGGTCCCTT AATGAAGAGT CACAAGCATT ACTAGCTCCT 
CCCTTACAGA GCCTTCACCC TGACTCTGCC CTGAAGGAAA ATAGCAAGGA TGTGAATGAA 
AAGGAAAATA ACATGGAGGA CATGGCTAAA ATATTCAAAT CTAAGGAGCA GCTCATGCTA 
AGGGCCAATA GTCTAAAGAA AGCTCTGAAA CAGATCATAG AGCAGGCAGA GAAAGCTGTG 
GATGAACAGA ACAAACACAC ACAAGTTTAC AGCACCCCCA TGTTATCCCC CATTCAGAAG 
GAGAGCTCTG AAGAATTCAG CAAAGAAGGA GAGAGTCTGG CTTCCAGGCG TGGAACTATC 
TCATCTACCA CATCCTCTAT CATTCTGGAC CGGCCGTATA CCTTCAGTCC CCTGCACTTT 
AGCGAGGATG CTATCCAGTT CTCAGAAAGA TGCACTATGA ATAATTATTT TGGCATTGGC 
CTGGATGCCA AGATATCGCT AGAGTTTAAT AACAAACGAG ATGAGCACCC CAAGAAGTGC 
AGCAGTCGGA CCCGGAACAT GATGTGGTAT GGTGTTCTGG GCACCAAGGA ACTTCTTCAG 
AAAACCTACA AGAATTTGGA GCAGCGTGTC AAGCTTGAGT GTGACGGGGA ACCCATCTCT 
CTGCCAAGCC TTCAGGGTAT TGCTGTTCTG AACATCCCCA GCTACGCTGG TGGCATAAAC 
TTCTGGGGAG GAACCAAAGA GGACAATAAT TTTGGGGCTC CATCTTTTGA CGACAAAAAG 
CTAGAGGTGG TGGCTGTGTT TGGGAGCATC CAGATGGCTG TGTCACGTGT AATCAATCTG 
CAGCACCATC GTATTGCTCA GTGCCGGGTA GTGAAGATCA CGATCCTCGG AGAAGAAGGT 
GTACCTGTGC AGGTGGATGG CGAGGCTTGG GTGCAGCCTC CTGGGATCCT GAAAATCCAA 
CACAAGAACC GGGCACAGAT GCTCACTAGA GACAGGGCTT TTGAGAACAC TCTGAAATCC 
TGGGAAGACA AGAGAAAGGG AGACAGTTAC AGGGTTATGG CACGGCCGAG GCTTCACTCC 
CAGCAGTCAA TGGAGTACCT TACAGAGGAA GAGTTTGCAC ATCTGCAGCA CTTGGCTAAC 
ACTTCTGAGA ATCTCATCAG CCGGATTCGG GAAGCTGCCA AGTATCACAA GGCTGTAGAG 
CAGGAGTTGG CCCATGCTGT CAATGGAAGC TTTGGGGTGC TCAATGAAGC ATTTTCCAAC 
AAAACCAACA ACTCTCCCGA GTTGCTGAGC AGAAATACGG CTGTGGATGT TGCCATAAGT 
GTGAAGGCAT TATATACAGA GACAAAGGCT TTCCTGGAGA GCAAGCAGCA ATGGGAATCT 
CCCCAGGAGG AGGAAGGGCT TCTCTGTTCC CTGAATGCAG TGGGCCAAGA CTTGGAGAGA 
CTGGCTGATA TCCACTGGAT CTCACACATC ATCAAACCAA CTGAGGAGGA GAGCAGTGGA 
GGAGGCAACA AGGGGAGCTT TAAACTGCGA ATCATTCCAA AACCAAAAAA GGAAAAGGAG 
AAGTGTCAGA AGCAGAAATC CAACAGCTCT CTAACAGTGG ATAAATGGGG CACAGAGGAG 
GTGGCTGCGT GGCTGGACGT TTTGGGTTTA GGGGAATACA AGGATATATT TATCCGCCAT 
GACATCCAAG GCTCAGAGCT GGTATTACTG GAAAGGAGAG ACCTCAAGGA CCTGGGAATA 
GCGAAAGTTG GTCATATGAA GAGAATTCTA CAGGGTATTA AGGATATAAC TACCCCTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_002939213.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939259.2 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.

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CloneID	OXa64561
Clone ID Related Accession (Same CDS sequence)	XM_002939213.4
Accession Version	XM_002939213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939213.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGTGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGG ACCTGGGAAT AGCGAAAGTT GGTCATATGA AGAGAATTCT 
ACAGGGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939259.2
CDS	349..3660
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTGPGNSESWSYEENSTGY

Target ORF information:

RefSeq Version	XM_002939213.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939213.4

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGTGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGG ACCTGGGAAT AGCGAAAGTT GGTCATATGA AGAGAATTCT 
ACAGGGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_031891294.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747154.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa64562
Clone ID Related Accession (Same CDS sequence)	XM_031891294.1
Accession Version	XM_031891294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT GGATTGGAGC CCTGAAATCA GTACAGAAAT GGGAGCTCAA TGAGACAACC 
CAGTTTAATA TGGAACACTT TTCTGGAATG CACAACTGGT ACGCTTGTTC CCATGCTCGT 
CCTACCTTCT GCAATGTGTG CAGAGAGGCA CTGTCTGGAG TCACATCCCA TGGGCTATCC 
TGTGAAGTCT GCAAGTTTAA AGCTCACAAG CGGTGCGCCG TAAGAGCCAC AAATAACTGC 
AAATGGACAA CACTAGCATC TGTGGGAAAT GACATCATAG AAGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGCTAGA AGGCAACCTG CCTGTCAGTG CCAAATGTGA AGTGTGTGAC 
AAGACATGTG GAAGTGTGCG GAGGCTGCAG GACTGGAGGT GCCTGTGGTG TAAAGCTATA 
GTGCACACAT CCTGTAAGGA GCAGCTGGGC AAAATCTGCC CTCTTGGACC ATGCAGAGTC 
TCTATTATCC CTCCTATTGC TCTTAATAGC ATTGACTCTG ATGGGTTCTG GAAGGCAACC 
TCACCTGCCT CGTGCGCCAG CCCCCTACTC GTCTTTGTGA ATTCTAAAAG CGGAGACAAC 
CAGGGTGTGA AATTTCTTCG AAAGTTCAAG CAGTTCCTTA ACCCCGCGCA GGTCTTTGAT 
CTCATGAATG GGGGGCCACA GCTGGGACTG CGCTTGTTCC AGAAATTCTC AAACTTTCGT 
ATACTGGTGT GTGGCGGAGA CGGCAGCGTT GGGTGGGTCT TGTCAGAGAT TGACAAGTTA 
GGACTGCAGA AACAGTGCCA GCTAGGAGTT CTGCCACTGG GCACAGGGAA TGACTTGGCA 
CGGGTGCTGG GTTGGGGAAG TCTATGTGAT GATGATGCTC AGCTACTCCA GATACTAGAG 
AAGCTGGAAC GTGCCACCAC CAAAATGCTT GACAGATGGA GCATATTAAC ATATGAATCC 
CCAAAGCACT CTCCCCCTGC CATCAGAGAG GAGGAGAATG GAGAGGCCAA ATTTCAGGTA 
CAGATATCCC AGTATGCCGA TTCTGTTGCC TTCCACCTGG CTAAAATTCT GGAATCAGAT 
AAGCACTGTG ATGTCATTTC ATCTGCCAAG TTCCTGTGCC TTTCAGTAAA TGATTTCATC 
ACTGAAGTTG GGAAGGCGTA CGCAAGGGCA GCAGAAAACC GTGAAGAGGC AGACAACATG 
GCTAGAAAGT GCTCAATGTT AAATGAGAAG CTGGAGTCTC TGGTTCGGTC CCTTAATGAA 
GAGTCACAAG CATTACTAGC TCCTCCCTTA CAGAGCCTTC ACCCTGACTC TGCCCTGAAG 
GAAAATAGCA AGGATGTGAA TGAAAAGGAA AATAACATGG AGGACATGGC TAAAATATTC 
AAATCTAAGG AGCAGCTCAT GCTAAGGGCC AATAGTCTAA AGAAAGCTCT GAAACAGATC 
ATAGAGCAGG CAGAGAAAGC TGTGGATGAA CAGAACAAAC ACACACAAGT TTACAGCACC 
CCCATGTTAT CCCCCATTCA GAAGGAGAGC TCTGAAGAAT TCAGCAAAGA AGGAGAGAGT 
CTGGCTTCCA GGCGTGGAAC TATCTCATCT ACCACATCCT CTATCATTCT GGACCGGCCG 
TATACCTTCA GTCCCCTGCA CTTTAGCGAG GATGCTATCC AGTTCTCAGA AAGATGCACT 
ATGAATAATT ATTTTGGCAT TGGCCTGGAT GCCAAGATAT CGCTAGAGTT TAATAACAAA 
CGAGATGAGC ACCCCAAGAA GTGCAGCAGT CGGACCCGGA ACATGATGTG GTATGGTGTT 
CTGGGCACCA AGGAACTTCT TCAGAAAACC TACAAGAATT TGGAGCAGCG TGTCAAGCTT 
GAGTGTGACG GGGAACCCAT CTCTCTGCCA AGCCTTCAGG GTATTGCTGT TCTGAACATC 
CCCAGCTACG CTGGTGGCAT AAACTTCTGG GGAGGAACCA AAGAGGACAA TAATTTTGGG 
GCTCCATCTT TTGACGACAA AAAGCTAGAG GTGGTGGCTG TGTTTGGGAG CATCCAGATG 
GCTGTGTCAC GTGTAATCAA TCTGCAGCAC CATCGTATTG CTCAGTGCCG GGTAGTGAAG 
ATCACGATCC TCGGAGAAGA AGGTGTACCT GTGCAGGTGG ATGGCGAGGC TTGGGTGCAG 
CCTCCTGGGA TCCTGAAAAT CCAACACAAG AACCGGGCAC AGATGCTCAC TAGAGACAGG 
GCTTTTGAGA ACACTCTGAA ATCCTGGGAA GACAAGAGAA AGGGAGACAG TTACAGGGTT 
ATGGCACGGC CGAGGCTTCA CTCCCAGCAG TCAATGGAGT ACCTTACAGA GGAAGAGTTT 
GCACATCTGC AGCACTTGGC TAACACTTCT GAGAATCTCA TCAGCCGGAT TCGGGAAGCT 
GCCAAGTATC ACAAGGCTGT AGAGCAGGAG TTGGCCCATG CTGTCAATGG AAGCTTTGGG 
GTGCTCAATG AAGCATTTTC CAACAAAACC AACAACTCTC CCGAGTTGCT GAGCAGAAAT 
ACGGCTGTGG ATGTTGCCAT AAGTGTGAAG GCATTATATA CAGAGACAAA GGCTTTCCTG 
GAGAGCAAGC AGCAATGGGA ATCTCCCCAG GAGGAGGAAG GGCTTCTCTG TTCCCTGAAT 
GCAGTGGGCC AAGACTTGGA GAGACTGGCT GATATCCACT GGATCTCACA CATCATCAAA 
CCAACTGAGG AGGAGAGCAG TGGAGGAGGC AACAAGGGGA GCTTTAAACT GCGAATCATT 
CCAAAACCAA AAAAGGAAAA GGAGAAGTGT CAGAAGCAGA AATCCAACAG CTCTCTAACA 
GTGGATAAAT GGGGCACAGA GGAGGTGGCT GCGTGGCTGG ACGTTTTGGG TTTAGGGGAA 
TACAAGGATA TATTTATCCG CCATGACATC CAAGGCTCAG AGCTGGTATT ACTGGAAAGG 
AGAGACCTCA AGGACCTGGG AATAGCGAAA GTTGGTCATA TGAAGAGAAT TCTACAGGGT 
ATTAAGGATA TAACTACCCC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747154.1
CDS	320..3346
Translation	MEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_031891294.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891294.1

ORF Insert Sequence:

ATGGAGGACT GGATTGGAGC CCTGAAATCA GTACAGAAAT GGGAGCTCAA TGAGACAACC 
CAGTTTAATA TGGAACACTT TTCTGGAATG CACAACTGGT ACGCTTGTTC CCATGCTCGT 
CCTACCTTCT GCAATGTGTG CAGAGAGGCA CTGTCTGGAG TCACATCCCA TGGGCTATCC 
TGTGAAGTCT GCAAGTTTAA AGCTCACAAG CGGTGCGCCG TAAGAGCCAC AAATAACTGC 
AAATGGACAA CACTAGCATC TGTGGGAAAT GACATCATAG AAGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGCTAGA AGGCAACCTG CCTGTCAGTG CCAAATGTGA AGTGTGTGAC 
AAGACATGTG GAAGTGTGCG GAGGCTGCAG GACTGGAGGT GCCTGTGGTG TAAAGCTATA 
GTGCACACAT CCTGTAAGGA GCAGCTGGGC AAAATCTGCC CTCTTGGACC ATGCAGAGTC 
TCTATTATCC CTCCTATTGC TCTTAATAGC ATTGACTCTG ATGGGTTCTG GAAGGCAACC 
TCACCTGCCT CGTGCGCCAG CCCCCTACTC GTCTTTGTGA ATTCTAAAAG CGGAGACAAC 
CAGGGTGTGA AATTTCTTCG AAAGTTCAAG CAGTTCCTTA ACCCCGCGCA GGTCTTTGAT 
CTCATGAATG GGGGGCCACA GCTGGGACTG CGCTTGTTCC AGAAATTCTC AAACTTTCGT 
ATACTGGTGT GTGGCGGAGA CGGCAGCGTT GGGTGGGTCT TGTCAGAGAT TGACAAGTTA 
GGACTGCAGA AACAGTGCCA GCTAGGAGTT CTGCCACTGG GCACAGGGAA TGACTTGGCA 
CGGGTGCTGG GTTGGGGAAG TCTATGTGAT GATGATGCTC AGCTACTCCA GATACTAGAG 
AAGCTGGAAC GTGCCACCAC CAAAATGCTT GACAGATGGA GCATATTAAC ATATGAATCC 
CCAAAGCACT CTCCCCCTGC CATCAGAGAG GAGGAGAATG GAGAGGCCAA ATTTCAGGTA 
CAGATATCCC AGTATGCCGA TTCTGTTGCC TTCCACCTGG CTAAAATTCT GGAATCAGAT 
AAGCACTGTG ATGTCATTTC ATCTGCCAAG TTCCTGTGCC TTTCAGTAAA TGATTTCATC 
ACTGAAGTTG GGAAGGCGTA CGCAAGGGCA GCAGAAAACC GTGAAGAGGC AGACAACATG 
GCTAGAAAGT GCTCAATGTT AAATGAGAAG CTGGAGTCTC TGGTTCGGTC CCTTAATGAA 
GAGTCACAAG CATTACTAGC TCCTCCCTTA CAGAGCCTTC ACCCTGACTC TGCCCTGAAG 
GAAAATAGCA AGGATGTGAA TGAAAAGGAA AATAACATGG AGGACATGGC TAAAATATTC 
AAATCTAAGG AGCAGCTCAT GCTAAGGGCC AATAGTCTAA AGAAAGCTCT GAAACAGATC 
ATAGAGCAGG CAGAGAAAGC TGTGGATGAA CAGAACAAAC ACACACAAGT TTACAGCACC 
CCCATGTTAT CCCCCATTCA GAAGGAGAGC TCTGAAGAAT TCAGCAAAGA AGGAGAGAGT 
CTGGCTTCCA GGCGTGGAAC TATCTCATCT ACCACATCCT CTATCATTCT GGACCGGCCG 
TATACCTTCA GTCCCCTGCA CTTTAGCGAG GATGCTATCC AGTTCTCAGA AAGATGCACT 
ATGAATAATT ATTTTGGCAT TGGCCTGGAT GCCAAGATAT CGCTAGAGTT TAATAACAAA 
CGAGATGAGC ACCCCAAGAA GTGCAGCAGT CGGACCCGGA ACATGATGTG GTATGGTGTT 
CTGGGCACCA AGGAACTTCT TCAGAAAACC TACAAGAATT TGGAGCAGCG TGTCAAGCTT 
GAGTGTGACG GGGAACCCAT CTCTCTGCCA AGCCTTCAGG GTATTGCTGT TCTGAACATC 
CCCAGCTACG CTGGTGGCAT AAACTTCTGG GGAGGAACCA AAGAGGACAA TAATTTTGGG 
GCTCCATCTT TTGACGACAA AAAGCTAGAG GTGGTGGCTG TGTTTGGGAG CATCCAGATG 
GCTGTGTCAC GTGTAATCAA TCTGCAGCAC CATCGTATTG CTCAGTGCCG GGTAGTGAAG 
ATCACGATCC TCGGAGAAGA AGGTGTACCT GTGCAGGTGG ATGGCGAGGC TTGGGTGCAG 
CCTCCTGGGA TCCTGAAAAT CCAACACAAG AACCGGGCAC AGATGCTCAC TAGAGACAGG 
GCTTTTGAGA ACACTCTGAA ATCCTGGGAA GACAAGAGAA AGGGAGACAG TTACAGGGTT 
ATGGCACGGC CGAGGCTTCA CTCCCAGCAG TCAATGGAGT ACCTTACAGA GGAAGAGTTT 
GCACATCTGC AGCACTTGGC TAACACTTCT GAGAATCTCA TCAGCCGGAT TCGGGAAGCT 
GCCAAGTATC ACAAGGCTGT AGAGCAGGAG TTGGCCCATG CTGTCAATGG AAGCTTTGGG 
GTGCTCAATG AAGCATTTTC CAACAAAACC AACAACTCTC CCGAGTTGCT GAGCAGAAAT 
ACGGCTGTGG ATGTTGCCAT AAGTGTGAAG GCATTATATA CAGAGACAAA GGCTTTCCTG 
GAGAGCAAGC AGCAATGGGA ATCTCCCCAG GAGGAGGAAG GGCTTCTCTG TTCCCTGAAT 
GCAGTGGGCC AAGACTTGGA GAGACTGGCT GATATCCACT GGATCTCACA CATCATCAAA 
CCAACTGAGG AGGAGAGCAG TGGAGGAGGC AACAAGGGGA GCTTTAAACT GCGAATCATT 
CCAAAACCAA AAAAGGAAAA GGAGAAGTGT CAGAAGCAGA AATCCAACAG CTCTCTAACA 
GTGGATAAAT GGGGCACAGA GGAGGTGGCT GCGTGGCTGG ACGTTTTGGG TTTAGGGGAA 
TACAAGGATA TATTTATCCG CCATGACATC CAAGGCTCAG AGCTGGTATT ACTGGAAAGG 
AGAGACCTCA AGGACCTGGG AATAGCGAAA GTTGGTCATA TGAAGAGAAT TCTACAGGGT 
ATTAAGGATA TAACTACCCC TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916779.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916836.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa14246
Clone ID Related Accession (Same CDS sequence)	XM_004916779.3
Accession Version	XM_004916779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGCGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGT GGATAAATGG GGCACAGAGG AGGTGGCTGC GTGGCTGGAC 
GTTTTGGGTT TAGGGGAATA CAAGGATATA TTTATCCGCC ATGACATCCA AGGCTCAGAG 
CTGGTATTAC TGGAAAGGAG AGACCTCAAG GACCTGGGAA TAGCGAAAGT TGGTCATATG 
AAGAGAATTC TACAGGGTAT TAAGGATATA ACTACCCCTA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916836.1
CDS	349..3813
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916779.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916779.3

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGCGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGT GGATAAATGG GGCACAGAGG AGGTGGCTGC GTGGCTGGAC 
GTTTTGGGTT TAGGGGAATA CAAGGATATA TTTATCCGCC ATGACATCCA AGGCTCAGAG 
CTGGTATTAC TGGAAAGGAG AGACCTCAAG GACCTGGGAA TAGCGAAAGT TGGTCATATG 
AAGAGAATTC TACAGGGTAT TAAGGATATA ACTACCCCTA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916780.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916837.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.

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CloneID	OXa14247
Clone ID Related Accession (Same CDS sequence)	XM_004916780.3
Accession Version	XM_004916780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGATATC CCAGTATGCC GATTCTGTTG CCTTCCACCT GGCTAAAATT 
CTGGAATCAG ATAAGCACTG CGATGTCATT TCATCTGCCA AGTTCCTGTG CCTTTCAGTA 
AATGATTTCA TCACTGAAGT TGGGAAGGCG TACGCAAGGG CAGCAGAAAA CCGTGAAGAG 
GCAGACAACA TGGCTAGAAA GTGCTCAATG TTAAATGAGA AGCTGGAGTC TCTGGTTCGG 
TCCCTTAATG AAGAGTCACA AGCATTACTA GCTCCTCCCT TACAGAGCCT TCACCCTGAC 
TCTGCCCTGA AGGAAAATAG CAAGGATGTG AATGAAAAGG AAAATAACAT GGAGGACATG 
GCTAAAATAT TCAAATCTAA GGAGCAGCTC ATGCTAAGGG CCAATAGTCT AAAGAAAGCT 
CTGAAACAGA TCATAGAGCA GGCAGAGAAA GCTGTGGATG AACAGAACAA ACACACACAA 
GTTTACAGCA CCCCCATGTT ATCCCCCATT CAGAAGGAGA GCTCTGAAGA ATTCAGCAAA 
GAAGGAGAGA GTCTGGCTTC CAGGCGTGGA ACTATCTCAT CTACCACATC CTCTATCATT 
CTGGACCGGC CGTATACCTT CAGTCCCCTG CACTTTAGCG AGGATGCTAT CCAGTTCTCA 
GAAAGATGCA CTATGAATAA TTATTTTGGC ATTGGCCTGG ATGCCAAGAT ATCGCTAGAG 
TTTAATAACA AACGAGATGA GCACCCCAAG AAGTGCAGCA GTCGGACCCG GAACATGATG 
TGGTATGGTG TTCTGGGCAC CAAGGAACTT CTTCAGAAAA CCTACAAGAA TTTGGAGCAG 
CGTGTCAAGC TTGAGTGTGA CGGGGAACCC ATCTCTCTGC CAAGCCTTCA GGGTATTGCT 
GTTCTGAACA TCCCCAGCTA CGCTGGTGGC ATAAACTTCT GGGGAGGAAC CAAAGAGGAC 
AATAATTTTG GGGCTCCATC TTTTGACGAC AAAAAGCTAG AGGTGGTGGC TGTGTTTGGG 
AGCATCCAGA TGGCTGTGTC ACGTGTAATC AATCTGCAGC ACCATCGTAT TGCTCAGTGC 
CGGGTAGTGA AGATCACGAT CCTCGGAGAA GAAGGTGTAC CTGTGCAGGT GGATGGCGAG 
GCTTGGGTGC AGCCTCCTGG GATCCTGAAA ATCCAACACA AGAACCGGGC ACAGATGCTC 
ACTAGAGACA GGGCTTTTGA GAACACTCTG AAATCCTGGG AAGACAAGAG AAAGGGAGAC 
AGTTACAGGG TTATGGCACG GCCGAGGCTT CACTCCCAGC AGTCAATGGA GTACCTTACA 
GAGGAAGAGT TTGCACATCT GCAGCACTTG GCTAACACTT CTGAGAATCT CATCAGCCGG 
ATTCGGGAAG CTGCCAAGTA TCACAAGGCT GTAGAGCAGG AGTTGGCCCA TGCTGTCAAT 
GGAAGCTTTG GGGTGCTCAA TGAAGCATTT TCCAACAAAA CCAACAACTC TCCCGAGTTG 
CTGAGCAGAA ATACGGCTGT GGATGTTGCC ATAAGTGTGA AGGCATTATA TACAGAGACA 
AAGGCTTTCC TGGAGAGCAA GCAGCAATGG GAATCTCCCC AGGAGGAGGA AGGGCTTCTC 
TGTTCCCTGA ATGCAGTGGG CCAAGACTTG GAGAGACTGG CTGATATCCA CTGGATCTCA 
CACATCATCA AACCAACTGA GGAGGAGAGC AGTGGAGGAG GCAACAAGGG GAGCTTTAAA 
CTGCGAATCA TTCCAAAACC AAAAAAGGAA AAGGAGAAGT GTCAGAAGCA GAAATCCAAC 
AGCTCTCTAA CAGTGGATAA ATGGGGCACA GAGGAGGTGG CTGCGTGGCT GGACGTTTTG 
GGTTTAGGGG AATACAAGGA TATATTTATC CGCCATGACA TCCAAGGCTC AGAGCTGGTA 
TTACTGGAAA GGAGAGACCT CAAGGACCTG GGAATAGCGA AAGTTGGTCA TATGAAGAGA 
ATTCTACAGG GTATTAAGGA TATAACTACC CCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916837.1
CDS	349..3807
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916780.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916780.3

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGATATC CCAGTATGCC GATTCTGTTG CCTTCCACCT GGCTAAAATT 
CTGGAATCAG ATAAGCACTG CGATGTCATT TCATCTGCCA AGTTCCTGTG CCTTTCAGTA 
AATGATTTCA TCACTGAAGT TGGGAAGGCG TACGCAAGGG CAGCAGAAAA CCGTGAAGAG 
GCAGACAACA TGGCTAGAAA GTGCTCAATG TTAAATGAGA AGCTGGAGTC TCTGGTTCGG 
TCCCTTAATG AAGAGTCACA AGCATTACTA GCTCCTCCCT TACAGAGCCT TCACCCTGAC 
TCTGCCCTGA AGGAAAATAG CAAGGATGTG AATGAAAAGG AAAATAACAT GGAGGACATG 
GCTAAAATAT TCAAATCTAA GGAGCAGCTC ATGCTAAGGG CCAATAGTCT AAAGAAAGCT 
CTGAAACAGA TCATAGAGCA GGCAGAGAAA GCTGTGGATG AACAGAACAA ACACACACAA 
GTTTACAGCA CCCCCATGTT ATCCCCCATT CAGAAGGAGA GCTCTGAAGA ATTCAGCAAA 
GAAGGAGAGA GTCTGGCTTC CAGGCGTGGA ACTATCTCAT CTACCACATC CTCTATCATT 
CTGGACCGGC CGTATACCTT CAGTCCCCTG CACTTTAGCG AGGATGCTAT CCAGTTCTCA 
GAAAGATGCA CTATGAATAA TTATTTTGGC ATTGGCCTGG ATGCCAAGAT ATCGCTAGAG 
TTTAATAACA AACGAGATGA GCACCCCAAG AAGTGCAGCA GTCGGACCCG GAACATGATG 
TGGTATGGTG TTCTGGGCAC CAAGGAACTT CTTCAGAAAA CCTACAAGAA TTTGGAGCAG 
CGTGTCAAGC TTGAGTGTGA CGGGGAACCC ATCTCTCTGC CAAGCCTTCA GGGTATTGCT 
GTTCTGAACA TCCCCAGCTA CGCTGGTGGC ATAAACTTCT GGGGAGGAAC CAAAGAGGAC 
AATAATTTTG GGGCTCCATC TTTTGACGAC AAAAAGCTAG AGGTGGTGGC TGTGTTTGGG 
AGCATCCAGA TGGCTGTGTC ACGTGTAATC AATCTGCAGC ACCATCGTAT TGCTCAGTGC 
CGGGTAGTGA AGATCACGAT CCTCGGAGAA GAAGGTGTAC CTGTGCAGGT GGATGGCGAG 
GCTTGGGTGC AGCCTCCTGG GATCCTGAAA ATCCAACACA AGAACCGGGC ACAGATGCTC 
ACTAGAGACA GGGCTTTTGA GAACACTCTG AAATCCTGGG AAGACAAGAG AAAGGGAGAC 
AGTTACAGGG TTATGGCACG GCCGAGGCTT CACTCCCAGC AGTCAATGGA GTACCTTACA 
GAGGAAGAGT TTGCACATCT GCAGCACTTG GCTAACACTT CTGAGAATCT CATCAGCCGG 
ATTCGGGAAG CTGCCAAGTA TCACAAGGCT GTAGAGCAGG AGTTGGCCCA TGCTGTCAAT 
GGAAGCTTTG GGGTGCTCAA TGAAGCATTT TCCAACAAAA CCAACAACTC TCCCGAGTTG 
CTGAGCAGAA ATACGGCTGT GGATGTTGCC ATAAGTGTGA AGGCATTATA TACAGAGACA 
AAGGCTTTCC TGGAGAGCAA GCAGCAATGG GAATCTCCCC AGGAGGAGGA AGGGCTTCTC 
TGTTCCCTGA ATGCAGTGGG CCAAGACTTG GAGAGACTGG CTGATATCCA CTGGATCTCA 
CACATCATCA AACCAACTGA GGAGGAGAGC AGTGGAGGAG GCAACAAGGG GAGCTTTAAA 
CTGCGAATCA TTCCAAAACC AAAAAAGGAA AAGGAGAAGT GTCAGAAGCA GAAATCCAAC 
AGCTCTCTAA CAGTGGATAA ATGGGGCACA GAGGAGGTGG CTGCGTGGCT GGACGTTTTG 
GGTTTAGGGG AATACAAGGA TATATTTATC CGCCATGACA TCCAAGGCTC AGAGCTGGTA 
TTACTGGAAA GGAGAGACCT CAAGGACCTG GGAATAGCGA AAGTTGGTCA TATGAAGAGA 
ATTCTACAGG GTATTAAGGA TATAACTACC CCTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_004916781.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916838.1 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa14248
Clone ID Related Accession (Same CDS sequence)	XM_004916781.3
Accession Version	XM_004916781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTTA TTTCAGACAC ATATCAACTT GGAGTAGGGC ATTCTTTTAC AACCTCTGTG 
CATGAAGAAG CCCCCAGGTT CTGGTGGAGT CTGGCGTGGC AGAACCCTAT CTTTATTATG 
TTGGAGAGAA CCCAAGATCA TCCATTTTTT CAGAAGAGCG TGAAAGAGGG AATCCTGCTA 
AAACAGACCA GCTCATTCCA GAGGTGGAAA AGGCGATATT TTAAACTGCG CGGAAGAACA 
CTTTACTATG CTAAGGATTC AAAGTCTCTC ATTTTTGATG AAGTTGATCT CTCAGATGCT 
AGTGTGGCTG AAACCAGTAC CAAGAATATT AATAACAGCT TCACGGTCAT CACCCCATTC 
CGCAAGCTTA TCCTGTGTGC TGAGAACAGA AAGGAGATGG AGGACTGGAT TGGAGCCCTG 
AAATCAGTAC AGAAATGGGA GCTCAATGAG ACAACCCAGT TTAATATGGA ACACTTTTCT 
GGAATGCACA ACTGGTACGC TTGTTCCCAT GCTCGTCCTA CCTTCTGCAA TGTGTGCAGA 
GAGGCACTGT CTGGAGTCAC ATCCCATGGG CTATCCTGTG AAGTCTGCAA GTTTAAAGCT 
CACAAGCGGT GCGCCGTAAG AGCCACAAAT AACTGCAAAT GGACAACACT AGCATCTGTG 
GGAAATGACA TCATAGAAGA TGAGGATGGA GTGTCAATGC CTCACCAGTG GCTAGAAGGC 
AACCTGCCTG TCAGTGCCAA ATGTGAAGTG TGTGACAAGA CATGTGGAAG TGTGCGGAGG 
CTGCAGGACT GGAGGTGCCT GTGGTGTAAA GCTATAGTGC ACACATCCTG TAAGGAGCAG 
CTGGGCAAAA TCTGCCCTCT TGGACCATGC AGAGTCTCTA TTATCCCTCC TATTGCTCTT 
AATAGCATTG ACTCTGATGG GTTCTGGAAG GCAACCTCAC CTGCCTCGTG CGCCAGCCCC 
CTACTCGTCT TTGTGAATTC TAAAAGCGGA GACAACCAGG GTGTGAAATT TCTTCGAAAG 
TTCAAGCAGT TCCTTAACCC CGCGCAGGTC TTTGATCTCA TGAATGGGGG GCCACAGCTG 
GGACTGCGCT TGTTCCAGAA ATTCTCAAAC TTTCGTATAC TGGTGTGTGG CGGAGACGGC 
AGCGTTGGGT GGGTCTTGTC AGAGATTGAC AAGTTAGGAC TGCAGAAACA GTGCCAGCTA 
GGAGTTCTGC CACTGGGCAC AGGGAATGAC TTGGCACGGG TGCTGGGTTG GGGAAGTCTA 
TGTGATGATG ATGCTCAGCT ACTCCAGATA CTAGAGAAGC TGGAACGTGC CACCACCAAA 
ATGCTTGACA GATGGAGCAT ATTAACATAT GAATCCCCAA AGCACTCTCC CCCTGCCATC 
AGAGAGGAGG AGAATGGAGA GGCCAAATTT CAGGTACAGA TATCCCAGTA TGCCGATTCT 
GTTGCCTTCC ACCTGGCTAA AATTCTGGAA TCAGATAAGC ACTGCGATGT CATTTCATCT 
GCCAAGTTCC TGTGCCTTTC AGTAAATGAT TTCATCACTG AAGTTGGGAA GGCGTACGCA 
AGGGCAGCAG AAAACCGTGA AGAGGCAGAC AACATGGCTA GAAAGTGCTC AATGTTAAAT 
GAGAAGCTGG AGTCTCTGGT TCGGTCCCTT AATGAAGAGT CACAAGCATT ACTAGCTCCT 
CCCTTACAGA GCCTTCACCC TGACTCTGCC CTGAAGGAAA ATAGCAAGGA TGTGAATGAA 
AAGGAAAATA ACATGGAGGA CATGGCTAAA ATATTCAAAT CTAAGGAGCA GCTCATGCTA 
AGGGCCAATA GTCTAAAGAA AGCTCTGAAA CAGATCATAG AGCAGGCAGA GAAAGCTGTG 
GATGAACAGA ACAAACACAC ACAAGTTTAC AGCACCCCCA TGTTATCCCC CATTCAGAAG 
GAGAGCTCTG AAGAATTCAG CAAAGAAGGA GAGAGTCTGG CTTCCAGGCG TGGAACTATC 
TCATCTACCA CATCCTCTAT CATTCTGGAC CGGCCGTATA CCTTCAGTCC CCTGCACTTT 
AGCGAGGATG CTATCCAGTT CTCAGAAAGA TGCACTATGA ATAATTATTT TGGCATTGGC 
CTGGATGCCA AGATATCGCT AGAGTTTAAT AACAAACGAG ATGAGCACCC CAAGAAGTGC 
AGCAGTCGGA CCCGGAACAT GATGTGGTAT GGTGTTCTGG GCACCAAGGA ACTTCTTCAG 
AAAACCTACA AGAATTTGGA GCAGCGTGTC AAGCTTGAGT GTGACGGGGA ACCCATCTCT 
CTGCCAAGCC TTCAGGGTAT TGCTGTTCTG AACATCCCCA GCTACGCTGG TGGCATAAAC 
TTCTGGGGAG GAACCAAAGA GGACAATAAT TTTGGGGCTC CATCTTTTGA CGACAAAAAG 
CTAGAGGTGG TGGCTGTGTT TGGGAGCATC CAGATGGCTG TGTCACGTGT AATCAATCTG 
CAGCACCATC GTATTGCTCA GTGCCGGGTA GTGAAGATCA CGATCCTCGG AGAAGAAGGT 
GTACCTGTGC AGGTGGATGG CGAGGCTTGG GTGCAGCCTC CTGGGATCCT GAAAATCCAA 
CACAAGAACC GGGCACAGAT GCTCACTAGA GACAGGGCTT TTGAGAACAC TCTGAAATCC 
TGGGAAGACA AGAGAAAGGG AGACAGTTAC AGGGTTATGG CACGGCCGAG GCTTCACTCC 
CAGCAGTCAA TGGAGTACCT TACAGAGGAA GAGTTTGCAC ATCTGCAGCA CTTGGCTAAC 
ACTTCTGAGA ATCTCATCAG CCGGATTCGG GAAGCTGCCA AGTATCACAA GGCTGTAGAG 
CAGGAGTTGG CCCATGCTGT CAATGGAAGC TTTGGGGTGC TCAATGAAGC ATTTTCCAAC 
AAAACCAACA ACTCTCCCGA GTTGCTGAGC AGAAATACGG CTGTGGATGT TGCCATAAGT 
GTGAAGGCAT TATATACAGA GACAAAGGCT TTCCTGGAGA GCAAGCAGCA ATGGGAATCT 
CCCCAGGAGG AGGAAGGGCT TCTCTGTTCC CTGAATGCAG TGGGCCAAGA CTTGGAGAGA 
CTGGCTGATA TCCACTGGAT CTCACACATC ATCAAACCAA CTGAGGAGGA GAGCAGTGGA 
GGAGGCAACA AGGGGAGCTT TAAACTGCGA ATCATTCCAA AACCAAAAAA GGAAAAGGAG 
AAGTGTCAGA AGCAGAAATC CAACAGCTCT CTAACAGTGG ATAAATGGGG CACAGAGGAG 
GTGGCTGCGT GGCTGGACGT TTTGGGTTTA GGGGAATACA AGGATATATT TATCCGCCAT 
GACATCCAAG GCTCAGAGCT GGTATTACTG GAAAGGAGAG ACCTCAAGGA CCTGGGAATA 
GCGAAAGTTG GTCATATGAA GAGAATTCTA CAGGGTATTA AGGATATAAC TACCCCTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916838.1
CDS	273..3695
Translation	MVLISDTYQLGVGHSFTTSVHEEAPRFWWSLAWQNPIFIMLERTQDHPFFQKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTVDKWGTEEVAAWLDVLGLGEYKDIFIRHDIQGSELVLLERRDLKDLGIAKVGHMKRILQGIKDITTPM

Target ORF information:

RefSeq Version	XM_004916781.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916781.3

ORF Insert Sequence:

ATGGTCCTTA TTTCAGACAC ATATCAACTT GGAGTAGGGC ATTCTTTTAC AACCTCTGTG 
CATGAAGAAG CCCCCAGGTT CTGGTGGAGT CTGGCGTGGC AGAACCCTAT CTTTATTATG 
TTGGAGAGAA CCCAAGATCA TCCATTTTTT CAGAAGAGCG TGAAAGAGGG AATCCTGCTA 
AAACAGACCA GCTCATTCCA GAGGTGGAAA AGGCGATATT TTAAACTGCG CGGAAGAACA 
CTTTACTATG CTAAGGATTC AAAGTCTCTC ATTTTTGATG AAGTTGATCT CTCAGATGCT 
AGTGTGGCTG AAACCAGTAC CAAGAATATT AATAACAGCT TCACGGTCAT CACCCCATTC 
CGCAAGCTTA TCCTGTGTGC TGAGAACAGA AAGGAGATGG AGGACTGGAT TGGAGCCCTG 
AAATCAGTAC AGAAATGGGA GCTCAATGAG ACAACCCAGT TTAATATGGA ACACTTTTCT 
GGAATGCACA ACTGGTACGC TTGTTCCCAT GCTCGTCCTA CCTTCTGCAA TGTGTGCAGA 
GAGGCACTGT CTGGAGTCAC ATCCCATGGG CTATCCTGTG AAGTCTGCAA GTTTAAAGCT 
CACAAGCGGT GCGCCGTAAG AGCCACAAAT AACTGCAAAT GGACAACACT AGCATCTGTG 
GGAAATGACA TCATAGAAGA TGAGGATGGA GTGTCAATGC CTCACCAGTG GCTAGAAGGC 
AACCTGCCTG TCAGTGCCAA ATGTGAAGTG TGTGACAAGA CATGTGGAAG TGTGCGGAGG 
CTGCAGGACT GGAGGTGCCT GTGGTGTAAA GCTATAGTGC ACACATCCTG TAAGGAGCAG 
CTGGGCAAAA TCTGCCCTCT TGGACCATGC AGAGTCTCTA TTATCCCTCC TATTGCTCTT 
AATAGCATTG ACTCTGATGG GTTCTGGAAG GCAACCTCAC CTGCCTCGTG CGCCAGCCCC 
CTACTCGTCT TTGTGAATTC TAAAAGCGGA GACAACCAGG GTGTGAAATT TCTTCGAAAG 
TTCAAGCAGT TCCTTAACCC CGCGCAGGTC TTTGATCTCA TGAATGGGGG GCCACAGCTG 
GGACTGCGCT TGTTCCAGAA ATTCTCAAAC TTTCGTATAC TGGTGTGTGG CGGAGACGGC 
AGCGTTGGGT GGGTCTTGTC AGAGATTGAC AAGTTAGGAC TGCAGAAACA GTGCCAGCTA 
GGAGTTCTGC CACTGGGCAC AGGGAATGAC TTGGCACGGG TGCTGGGTTG GGGAAGTCTA 
TGTGATGATG ATGCTCAGCT ACTCCAGATA CTAGAGAAGC TGGAACGTGC CACCACCAAA 
ATGCTTGACA GATGGAGCAT ATTAACATAT GAATCCCCAA AGCACTCTCC CCCTGCCATC 
AGAGAGGAGG AGAATGGAGA GGCCAAATTT CAGGTACAGA TATCCCAGTA TGCCGATTCT 
GTTGCCTTCC ACCTGGCTAA AATTCTGGAA TCAGATAAGC ACTGCGATGT CATTTCATCT 
GCCAAGTTCC TGTGCCTTTC AGTAAATGAT TTCATCACTG AAGTTGGGAA GGCGTACGCA 
AGGGCAGCAG AAAACCGTGA AGAGGCAGAC AACATGGCTA GAAAGTGCTC AATGTTAAAT 
GAGAAGCTGG AGTCTCTGGT TCGGTCCCTT AATGAAGAGT CACAAGCATT ACTAGCTCCT 
CCCTTACAGA GCCTTCACCC TGACTCTGCC CTGAAGGAAA ATAGCAAGGA TGTGAATGAA 
AAGGAAAATA ACATGGAGGA CATGGCTAAA ATATTCAAAT CTAAGGAGCA GCTCATGCTA 
AGGGCCAATA GTCTAAAGAA AGCTCTGAAA CAGATCATAG AGCAGGCAGA GAAAGCTGTG 
GATGAACAGA ACAAACACAC ACAAGTTTAC AGCACCCCCA TGTTATCCCC CATTCAGAAG 
GAGAGCTCTG AAGAATTCAG CAAAGAAGGA GAGAGTCTGG CTTCCAGGCG TGGAACTATC 
TCATCTACCA CATCCTCTAT CATTCTGGAC CGGCCGTATA CCTTCAGTCC CCTGCACTTT 
AGCGAGGATG CTATCCAGTT CTCAGAAAGA TGCACTATGA ATAATTATTT TGGCATTGGC 
CTGGATGCCA AGATATCGCT AGAGTTTAAT AACAAACGAG ATGAGCACCC CAAGAAGTGC 
AGCAGTCGGA CCCGGAACAT GATGTGGTAT GGTGTTCTGG GCACCAAGGA ACTTCTTCAG 
AAAACCTACA AGAATTTGGA GCAGCGTGTC AAGCTTGAGT GTGACGGGGA ACCCATCTCT 
CTGCCAAGCC TTCAGGGTAT TGCTGTTCTG AACATCCCCA GCTACGCTGG TGGCATAAAC 
TTCTGGGGAG GAACCAAAGA GGACAATAAT TTTGGGGCTC CATCTTTTGA CGACAAAAAG 
CTAGAGGTGG TGGCTGTGTT TGGGAGCATC CAGATGGCTG TGTCACGTGT AATCAATCTG 
CAGCACCATC GTATTGCTCA GTGCCGGGTA GTGAAGATCA CGATCCTCGG AGAAGAAGGT 
GTACCTGTGC AGGTGGATGG CGAGGCTTGG GTGCAGCCTC CTGGGATCCT GAAAATCCAA 
CACAAGAACC GGGCACAGAT GCTCACTAGA GACAGGGCTT TTGAGAACAC TCTGAAATCC 
TGGGAAGACA AGAGAAAGGG AGACAGTTAC AGGGTTATGG CACGGCCGAG GCTTCACTCC 
CAGCAGTCAA TGGAGTACCT TACAGAGGAA GAGTTTGCAC ATCTGCAGCA CTTGGCTAAC 
ACTTCTGAGA ATCTCATCAG CCGGATTCGG GAAGCTGCCA AGTATCACAA GGCTGTAGAG 
CAGGAGTTGG CCCATGCTGT CAATGGAAGC TTTGGGGTGC TCAATGAAGC ATTTTCCAAC 
AAAACCAACA ACTCTCCCGA GTTGCTGAGC AGAAATACGG CTGTGGATGT TGCCATAAGT 
GTGAAGGCAT TATATACAGA GACAAAGGCT TTCCTGGAGA GCAAGCAGCA ATGGGAATCT 
CCCCAGGAGG AGGAAGGGCT TCTCTGTTCC CTGAATGCAG TGGGCCAAGA CTTGGAGAGA 
CTGGCTGATA TCCACTGGAT CTCACACATC ATCAAACCAA CTGAGGAGGA GAGCAGTGGA 
GGAGGCAACA AGGGGAGCTT TAAACTGCGA ATCATTCCAA AACCAAAAAA GGAAAAGGAG 
AAGTGTCAGA AGCAGAAATC CAACAGCTCT CTAACAGTGG ATAAATGGGG CACAGAGGAG 
GTGGCTGCGT GGCTGGACGT TTTGGGTTTA GGGGAATACA AGGATATATT TATCCGCCAT 
GACATCCAAG GCTCAGAGCT GGTATTACTG GAAAGGAGAG ACCTCAAGGA CCTGGGAATA 
GCGAAAGTTG GTCATATGAA GAGAATTCTA CAGGGTATTA AGGATATAAC TACCCCTATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkk ( XM_002939213.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939259.2 Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.

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CloneID	OXa14249
Clone ID Related Accession (Same CDS sequence)	XM_002939213.3
Accession Version	XM_002939213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase kappa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGCGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGG ACCTGGGAAT AGCGAAAGTT GGTCATATGA AGAGAATTCT 
ACAGGGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939259.2
CDS	349..3660
Translation	MAQKLSPDDAFKRRARDSVSSLDSDKLPTAEATGEESSDSEGEEQEGISHKLIRKVSTSGQIRSKKSVKEGILLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLILCAENRKEMEDWIGALKSVQKWELNETTQFNMEHFSGMHNWYACSHARPTFCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASVGNDIIEDEDGVSMPHQWLEGNLPVSAKCEVCDKTCGSVRRLQDWRCLWCKAIVHTSCKEQLGKICPLGPCRVSIIPPIALNSIDSDGFWKATSPASCASPLLVFVNSKSGDNQGVKFLRKFKQFLNPAQVFDLMNGGPQLGLRLFQKFSNFRILVCGGDGSVGWVLSEIDKLGLQKQCQLGVLPLGTGNDLARVLGWGSLCDDDAQLLQILEKLERATTKMLDRWSILTYESPKHSPPAIREEENGEAKFQVQISQYADSVAFHLAKILESDKHCDVISSAKFLCLSVNDFITEVGKAYARAAENREEADNMARKCSMLNEKLESLVRSLNEESQALLAPPLQSLHPDSALKENSKDVNEKENNMEDMAKIFKSKEQLMLRANSLKKALKQIIEQAEKAVDEQNKHTQVYSTPMLSPIQKESSEEFSKEGESLASRRGTISSTTSSIILDRPYTFSPLHFSEDAIQFSERCTMNNYFGIGLDAKISLEFNNKRDEHPKKCSSRTRNMMWYGVLGTKELLQKTYKNLEQRVKLECDGEPISLPSLQGIAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSIQMAVSRVINLQHHRIAQCRVVKITILGEEGVPVQVDGEAWVQPPGILKIQHKNRAQMLTRDRAFENTLKSWEDKRKGDSYRVMARPRLHSQQSMEYLTEEEFAHLQHLANTSENLISRIREAAKYHKAVEQELAHAVNGSFGVLNEAFSNKTNNSPELLSRNTAVDVAISVKALYTETKAFLESKQQWESPQEEEGLLCSLNAVGQDLERLADIHWISHIIKPTEEESSGGGNKGSFKLRIIPKPKKEKEKCQKQKSNSSLTGPGNSESWSYEENSTGY

Target ORF information:

RefSeq Version	XM_002939213.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase kappa (dgkk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939213.3

ORF Insert Sequence:

ATGGCTCAGA AGCTGTCTCC AGATGATGCC TTTAAAAGAA GGGCACGGGA TTCTGTGTCT 
TCTCTTGATT CAGATAAACT GCCCACAGCA GAAGCAACTG GCGAGGAGTC ATCTGATAGT 
GAAGGAGAGG AACAAGAAGG TATTTCCCAC AAACTCATCC GCAAGGTGTC TACATCTGGA 
CAAATTCGGA GCAAGAAGAG CGTGAAAGAG GGAATCCTGC TAAAACAGAC CAGCTCATTC 
CAGAGGTGGA AAAGGCGATA TTTTAAACTG CGCGGAAGAA CACTTTACTA TGCTAAGGAT 
TCAAAGTCTC TCATTTTTGA TGAAGTTGAT CTCTCAGATG CTAGTGTGGC TGAAACCAGT 
ACCAAGAATA TTAATAACAG CTTCACGGTC ATCACCCCAT TCCGCAAGCT TATCCTGTGT 
GCTGAGAACA GAAAGGAGAT GGAGGACTGG ATTGGAGCCC TGAAATCAGT ACAGAAATGG 
GAGCTCAATG AGACAACCCA GTTTAATATG GAACACTTTT CTGGAATGCA CAACTGGTAC 
GCTTGTTCCC ATGCTCGTCC TACCTTCTGC AATGTGTGCA GAGAGGCACT GTCTGGAGTC 
ACATCCCATG GGCTATCCTG TGAAGTCTGC AAGTTTAAAG CTCACAAGCG GTGCGCCGTA 
AGAGCCACAA ATAACTGCAA ATGGACAACA CTAGCATCTG TGGGAAATGA CATCATAGAA 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGCTAGAAG GCAACCTGCC TGTCAGTGCC 
AAATGTGAAG TGTGTGACAA GACATGTGGA AGTGTGCGGA GGCTGCAGGA CTGGAGGTGC 
CTGTGGTGTA AAGCTATAGT GCACACATCC TGTAAGGAGC AGCTGGGCAA AATCTGCCCT 
CTTGGACCAT GCAGAGTCTC TATTATCCCT CCTATTGCTC TTAATAGCAT TGACTCTGAT 
GGGTTCTGGA AGGCAACCTC ACCTGCCTCG TGCGCCAGCC CCCTACTCGT CTTTGTGAAT 
TCTAAAAGCG GAGACAACCA GGGTGTGAAA TTTCTTCGAA AGTTCAAGCA GTTCCTTAAC 
CCCGCGCAGG TCTTTGATCT CATGAATGGG GGGCCACAGC TGGGACTGCG CTTGTTCCAG 
AAATTCTCAA ACTTTCGTAT ACTGGTGTGT GGCGGAGACG GCAGCGTTGG GTGGGTCTTG 
TCAGAGATTG ACAAGTTAGG ACTGCAGAAA CAGTGCCAGC TAGGAGTTCT GCCACTGGGC 
ACAGGGAATG ACTTGGCACG GGTGCTGGGT TGGGGAAGTC TATGTGATGA TGATGCTCAG 
CTACTCCAGA TACTAGAGAA GCTGGAACGT GCCACCACCA AAATGCTTGA CAGATGGAGC 
ATATTAACAT ATGAATCCCC AAAGCACTCT CCCCCTGCCA TCAGAGAGGA GGAGAATGGA 
GAGGCCAAAT TTCAGGTACA GATATCCCAG TATGCCGATT CTGTTGCCTT CCACCTGGCT 
AAAATTCTGG AATCAGATAA GCACTGCGAT GTCATTTCAT CTGCCAAGTT CCTGTGCCTT 
TCAGTAAATG ATTTCATCAC TGAAGTTGGG AAGGCGTACG CAAGGGCAGC AGAAAACCGT 
GAAGAGGCAG ACAACATGGC TAGAAAGTGC TCAATGTTAA ATGAGAAGCT GGAGTCTCTG 
GTTCGGTCCC TTAATGAAGA GTCACAAGCA TTACTAGCTC CTCCCTTACA GAGCCTTCAC 
CCTGACTCTG CCCTGAAGGA AAATAGCAAG GATGTGAATG AAAAGGAAAA TAACATGGAG 
GACATGGCTA AAATATTCAA ATCTAAGGAG CAGCTCATGC TAAGGGCCAA TAGTCTAAAG 
AAAGCTCTGA AACAGATCAT AGAGCAGGCA GAGAAAGCTG TGGATGAACA GAACAAACAC 
ACACAAGTTT ACAGCACCCC CATGTTATCC CCCATTCAGA AGGAGAGCTC TGAAGAATTC 
AGCAAAGAAG GAGAGAGTCT GGCTTCCAGG CGTGGAACTA TCTCATCTAC CACATCCTCT 
ATCATTCTGG ACCGGCCGTA TACCTTCAGT CCCCTGCACT TTAGCGAGGA TGCTATCCAG 
TTCTCAGAAA GATGCACTAT GAATAATTAT TTTGGCATTG GCCTGGATGC CAAGATATCG 
CTAGAGTTTA ATAACAAACG AGATGAGCAC CCCAAGAAGT GCAGCAGTCG GACCCGGAAC 
ATGATGTGGT ATGGTGTTCT GGGCACCAAG GAACTTCTTC AGAAAACCTA CAAGAATTTG 
GAGCAGCGTG TCAAGCTTGA GTGTGACGGG GAACCCATCT CTCTGCCAAG CCTTCAGGGT 
ATTGCTGTTC TGAACATCCC CAGCTACGCT GGTGGCATAA ACTTCTGGGG AGGAACCAAA 
GAGGACAATA ATTTTGGGGC TCCATCTTTT GACGACAAAA AGCTAGAGGT GGTGGCTGTG 
TTTGGGAGCA TCCAGATGGC TGTGTCACGT GTAATCAATC TGCAGCACCA TCGTATTGCT 
CAGTGCCGGG TAGTGAAGAT CACGATCCTC GGAGAAGAAG GTGTACCTGT GCAGGTGGAT 
GGCGAGGCTT GGGTGCAGCC TCCTGGGATC CTGAAAATCC AACACAAGAA CCGGGCACAG 
ATGCTCACTA GAGACAGGGC TTTTGAGAAC ACTCTGAAAT CCTGGGAAGA CAAGAGAAAG 
GGAGACAGTT ACAGGGTTAT GGCACGGCCG AGGCTTCACT CCCAGCAGTC AATGGAGTAC 
CTTACAGAGG AAGAGTTTGC ACATCTGCAG CACTTGGCTA ACACTTCTGA GAATCTCATC 
AGCCGGATTC GGGAAGCTGC CAAGTATCAC AAGGCTGTAG AGCAGGAGTT GGCCCATGCT 
GTCAATGGAA GCTTTGGGGT GCTCAATGAA GCATTTTCCA ACAAAACCAA CAACTCTCCC 
GAGTTGCTGA GCAGAAATAC GGCTGTGGAT GTTGCCATAA GTGTGAAGGC ATTATATACA 
GAGACAAAGG CTTTCCTGGA GAGCAAGCAG CAATGGGAAT CTCCCCAGGA GGAGGAAGGG 
CTTCTCTGTT CCCTGAATGC AGTGGGCCAA GACTTGGAGA GACTGGCTGA TATCCACTGG 
ATCTCACACA TCATCAAACC AACTGAGGAG GAGAGCAGTG GAGGAGGCAA CAAGGGGAGC 
TTTAAACTGC GAATCATTCC AAAACCAAAA AAGGAAAAGG AGAAGTGTCA GAAGCAGAAA 
TCCAACAGCT CTCTAACAGG ACCTGGGAAT AGCGAAAGTT GGTCATATGA AGAGAATTCT 
ACAGGGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	dgkk
Entrez Gene ID	100496859
Full Name	diacylglycerol kinase kappa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	si:dkey-172j4.3	XP_005172372.1
Gallus gallus (chicken)	DGKK	XP_420132.4
Xenopus tropicalis (tropical clawed frog)	LOC100496859	XP_004916837.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

dgkk cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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