kiaa1217 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012965644.2	XP_012821098.1	sickle tail protein homolog isoform X3
XM_012965645.2	XP_012821099.1	sickle tail protein homolog isoform X7
XM_018094778.1	XP_017950267.1	sickle tail protein homolog isoform X8
XM_004915443.3	XP_004915500.1	sickle tail protein homolog isoform X10
XM_012965648.2	XP_012821102.1	sickle tail protein homolog isoform X12
XM_004915438.4	XP_004915495.1	sickle tail protein homolog isoform X5
XM_012965645.3	XP_012821099.1	sickle tail protein homolog isoform X7
XM_012965649.3	XP_012821103.1	sickle tail protein homolog isoform X14
XM_004915435.3	XP_004915492.1	sickle tail protein homolog isoform X1
XM_012965647.2	XP_012821101.1	sickle tail protein homolog isoform X11
XM_012965649.2	XP_012821103.1	sickle tail protein homolog isoform X13
XM_002933147.5	XP_002933193.2	sickle tail protein homolog isoform X2
XM_004915436.4	XP_004915493.1	sickle tail protein homolog isoform X4
XM_012965646.3	XP_012821100.1	sickle tail protein homolog isoform X9
XM_031903539.1	XP_031759399.1	sickle tail protein homolog isoform X11
XM_002933147.4	XP_002933193.2	sickle tail protein homolog isoform X2
XM_004915439.3	XP_004915496.1	sickle tail protein homolog isoform X6
XM_004915435.4	XP_004915492.1	sickle tail protein homolog isoform X1
XM_012965644.3	XP_012821098.1	sickle tail protein homolog isoform X3
XM_004915436.3	XP_004915493.1	sickle tail protein homolog isoform X4
XM_004915438.3	XP_004915495.1	sickle tail protein homolog isoform X5
XM_004915440.3	XP_004915497.1	sickle tail protein homolog isoform X8
XM_012965646.2	XP_012821100.1	sickle tail protein homolog isoform X9
XM_004915439.4	XP_004915496.1	sickle tail protein homolog isoform X6
XM_012965647.3	XP_012821101.1	sickle tail protein homolog isoform X12
XM_012965648.3	XP_012821102.1	sickle tail protein homolog isoform X13
XM_004915440.4	XP_004915497.1	sickle tail protein homolog isoform X8
XM_018094778.2	XP_017950267.1	sickle tail protein homolog isoform X8
XM_004915443.4	XP_004915500.1	sickle tail protein homolog isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa36495	XM_012965647.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36496	XM_012965648.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa36497	XM_012965649.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa12038	XM_004915435.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12039	XM_002933147.5 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12040	XM_004915436.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12042	XM_004915438.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12043	XM_004915439.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12044	XM_018094778.2 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12044	XM_004915440.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa12045	XM_004915443.4 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa36492	XM_012965644.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa36493	XM_012965645.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa36494	XM_012965646.3 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa59883	XM_031903539.1 Latest version!	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa36493	XM_012965645.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36497	XM_012965649.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa12039	XM_002933147.4	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12038	XM_004915435.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36492	XM_012965644.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12040	XM_004915436.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12042	XM_004915438.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36494	XM_012965646.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12043	XM_004915439.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36495	XM_012965647.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa36496	XM_012965648.2	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa12044	XM_004915440.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12044	XM_018094778.1	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa12045	XM_004915443.3	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

kiaa1217 ( XM_012965647.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821101.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa36495
Clone ID Related Accession (Same CDS sequence)	XM_012965647.2 , XM_012965647.3
Accession Version	XM_012965647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965647.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGGTTC CAGTGGAGGA GCACAAACCA GCAGAATGCC AATCCCAATG 
TCTTCTAAAA GTAGGCAAGC AAACGTGGAC AAAAGTAGCA AACAGCCCAA ACTGCAGGAG 
CCGCAGCGCC AATACAGACA GGCTAACGGA GGTACTAAGA AAGCTGGTGG GGATGTTAAG 
GCTTCTGCCC CTTCTTTATC TGTTTCTAAG ATTCCAGGCC TTTCCTCTAA CACTGGGAAA 
AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG ACCTCTAACC CCCCTAATCC CTCTGTCAAG 
TCACATGTTG CCAATTCTAA CCCCCAAGCT AGCCGCACCG CTAACCCTTC AGCCCTTGTA 
CGTCCTGCTC ACAATGGCTC ATCCAAACTT CAGCCCCCGC CTACATATAA AGGTCACCAT 
CTTTCATTCT CACCGCAGGC CTCAAATGGC AGACCAGCTC TTCCCTCTAC TACTGCTTCT 
AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC ACTTCACTGA CTCAAGGTGT GAAGAGCATC 
CGCACAATCC ATACACCTAG CTTCACCAGC TACAAGTCAC AGAACGGAAG TGCAAGCAAA 
GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821101.1
CDS	315..4661
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965647.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965647.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGGTTC CAGTGGAGGA GCACAAACCA GCAGAATGCC AATCCCAATG 
TCTTCTAAAA GTAGGCAAGC AAACGTGGAC AAAAGTAGCA AACAGCCCAA ACTGCAGGAG 
CCGCAGCGCC AATACAGACA GGCTAACGGA GGTACTAAGA AAGCTGGTGG GGATGTTAAG 
GCTTCTGCCC CTTCTTTATC TGTTTCTAAG ATTCCAGGCC TTTCCTCTAA CACTGGGAAA 
AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG ACCTCTAACC CCCCTAATCC CTCTGTCAAG 
TCACATGTTG CCAATTCTAA CCCCCAAGCT AGCCGCACCG CTAACCCTTC AGCCCTTGTA 
CGTCCTGCTC ACAATGGCTC ATCCAAACTT CAGCCCCCGC CTACATATAA AGGTCACCAT 
CTTTCATTCT CACCGCAGGC CTCAAATGGC AGACCAGCTC TTCCCTCTAC TACTGCTTCT 
AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC ACTTCACTGA CTCAAGGTGT GAAGAGCATC 
CGCACAATCC ATACACCTAG CTTCACCAGC TACAAGTCAC AGAACGGAAG TGCAAGCAAA 
GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965648.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821102.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.

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CloneID	OXa36496
Clone ID Related Accession (Same CDS sequence)	XM_012965648.2 , XM_012965648.3
Accession Version	XM_012965648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGGTTCCAGT GGAGGAGCAC AAACCAGCAG AATGCCAATC 
CCAATGTCTT CTAAAAGTAG GCAAGCAAAC GTGGACAAAA GTAGCAAACA GCCCAAACTG 
CAGGAGCCGC AGCGCCAATA CAGACAGGCT AACGGAGGTA CTAAGAAAGC TGGTGGGGAT 
GTTAAGGCTT CTGCCCCTTC TTTATCTGTT TCTAAGATTC CAGGCCTTTC CTCTAACACT 
GGGAAAAGTG GCTCTGTGCC TGCTCAGTCT AGCGAGACCT CTAACCCCCC TAATCCCTCT 
GTCAAGTCAC ATGTTGCCAA TTCTAACCCC CAAGCTAGCC GCACCGCTAA CCCTTCAGCC 
CTTGTACGTC CTGCTCACAA TGGCTCATCC AAACTTCAGC CCCCGCCTAC ATATAAAGGT 
CACCATCTTT CATTCTCACC GCAGGCCTCA AATGGCAGAC CAGCTCTTCC CTCTACTACT 
GCTTCTAGTT CTTCTCCCCC TTCTTCTGTC TCTCCCACTT CACTGACTCA AGGTGTGAAG 
AGCATCCGCA CAATCCATAC ACCTAGCTTC ACCAGCTACA AGTCACAGAA CGGAAGTGCA 
AGCAAAGGCC TGAATGCCAA GGAGACCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821102.1
CDS	315..4547
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965648.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965648.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGGTTCCAGT GGAGGAGCAC AAACCAGCAG AATGCCAATC 
CCAATGTCTT CTAAAAGTAG GCAAGCAAAC GTGGACAAAA GTAGCAAACA GCCCAAACTG 
CAGGAGCCGC AGCGCCAATA CAGACAGGCT AACGGAGGTA CTAAGAAAGC TGGTGGGGAT 
GTTAAGGCTT CTGCCCCTTC TTTATCTGTT TCTAAGATTC CAGGCCTTTC CTCTAACACT 
GGGAAAAGTG GCTCTGTGCC TGCTCAGTCT AGCGAGACCT CTAACCCCCC TAATCCCTCT 
GTCAAGTCAC ATGTTGCCAA TTCTAACCCC CAAGCTAGCC GCACCGCTAA CCCTTCAGCC 
CTTGTACGTC CTGCTCACAA TGGCTCATCC AAACTTCAGC CCCCGCCTAC ATATAAAGGT 
CACCATCTTT CATTCTCACC GCAGGCCTCA AATGGCAGAC CAGCTCTTCC CTCTACTACT 
GCTTCTAGTT CTTCTCCCCC TTCTTCTGTC TCTCCCACTT CACTGACTCA AGGTGTGAAG 
AGCATCCGCA CAATCCATAC ACCTAGCTTC ACCAGCTACA AGTCACAGAA CGGAAGTGCA 
AGCAAAGGCC TGAATGCCAA GGAGACCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965649.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821103.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa36497
Clone ID Related Accession (Same CDS sequence)	XM_012965649.3 , XM_012965649.2
Accession Version	XM_012965649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGGTTCC 
AGTGGAGGAG CACAAACCAG CAGAATGCCA ATCCCAATGT CTTCTAAAAG TAGGCAAGCA 
AACGTGGACA AAAGTAGCAA ACAGCCCAAA CTGCAGGAGC CGCAGCGCCA ATACAGACAG 
GCTAACGGAG GTACTAAGAA AGCTGGTGGG GATGTTAAGG CTTCTGCCCC TTCTTTATCT 
GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG 
TCTAGCGAGA CCTCTAACCC CCCTAATCCC TCTGTCAAGT CACATGTTGC CAATTCTAAC 
CCCCAAGCTA GCCGCACCGC TAACCCTTCA GCCCTTGTAC GTCCTGCTCA CAATGGCTCA 
TCCAAACTTC AGCCCCCGCC TACATATAAA GGTCACCATC TTTCATTCTC ACCGCAGGCC 
TCAAATGGCA GACCAGCTCT TCCCTCTACT ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT 
GTCTCTCCCA CTTCACTGAC TCAAGGTGTG AAGAGCATCC GCACAATCCA TACACCTAGC 
TTCACCAGCT ACAAGTCACA GAACGGAAGT GCAAGCAAAG GCCTGAATGC CAAGGAGACC 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821103.1
CDS	314..4579
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965649.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965649.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGGTTCC 
AGTGGAGGAG CACAAACCAG CAGAATGCCA ATCCCAATGT CTTCTAAAAG TAGGCAAGCA 
AACGTGGACA AAAGTAGCAA ACAGCCCAAA CTGCAGGAGC CGCAGCGCCA ATACAGACAG 
GCTAACGGAG GTACTAAGAA AGCTGGTGGG GATGTTAAGG CTTCTGCCCC TTCTTTATCT 
GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG 
TCTAGCGAGA CCTCTAACCC CCCTAATCCC TCTGTCAAGT CACATGTTGC CAATTCTAAC 
CCCCAAGCTA GCCGCACCGC TAACCCTTCA GCCCTTGTAC GTCCTGCTCA CAATGGCTCA 
TCCAAACTTC AGCCCCCGCC TACATATAAA GGTCACCATC TTTCATTCTC ACCGCAGGCC 
TCAAATGGCA GACCAGCTCT TCCCTCTACT ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT 
GTCTCTCCCA CTTCACTGAC TCAAGGTGTG AAGAGCATCC GCACAATCCA TACACCTAGC 
TTCACCAGCT ACAAGTCACA GAACGGAAGT GCAAGCAAAG GCCTGAATGC CAAGGAGACC 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915435.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915492.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.

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CloneID	OXa12038
Clone ID Related Accession (Same CDS sequence)	XM_004915435.3 , XM_004915435.4
Accession Version	XM_004915435.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915435.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGTAAGGGT TCCAGTGGAG GAGCACAAAC CAGCAGAATG 
CCAATCCCAA TGTCTTCTAA AAGTAGGCAA GCAAACGTGG ACAAAAGTAG CAAACAGCCC 
AAACTGCAGG AGCCGCAGCG CCAATACAGA CAGGCTAACG GAGGTACTAA GAAAGCTGGT 
GGGGATGTTA AGGCTTCTGC CCCTTCTTTA TCTGTTTCTA AGATTCCAGG CCTTTCCTCT 
AACACTGGGA AAAGTGGCTC TGTGCCTGCT CAGTCTAGCG AGACCTCTAA CCCCCCTAAT 
CCCTCTGTCA AGTCACATGT TGCCAATTCT AACCCCCAAG CTAGCCGCAC CGCTAACCCT 
TCAGCCCTTG TACGTCCTGC TCACAATGGC TCATCCAAAC TTCAGCCCCC GCCTACATAT 
AAAGGTCACC ATCTTTCATT CTCACCGCAG GCCTCAAATG GCAGACCAGC TCTTCCCTCT 
ACTACTGCTT CTAGTTCTTC TCCCCCTTCT TCTGTCTCTC CCACTTCACT GACTCAAGGT 
GTGAAGAGCA TCCGCACAAT CCATACACCT AGCTTCACCA GCTACAAGTC ACAGAACGGA 
AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915492.1
CDS	316..6234
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915435.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915435.4

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGTAAGGGT TCCAGTGGAG GAGCACAAAC CAGCAGAATG 
CCAATCCCAA TGTCTTCTAA AAGTAGGCAA GCAAACGTGG ACAAAAGTAG CAAACAGCCC 
AAACTGCAGG AGCCGCAGCG CCAATACAGA CAGGCTAACG GAGGTACTAA GAAAGCTGGT 
GGGGATGTTA AGGCTTCTGC CCCTTCTTTA TCTGTTTCTA AGATTCCAGG CCTTTCCTCT 
AACACTGGGA AAAGTGGCTC TGTGCCTGCT CAGTCTAGCG AGACCTCTAA CCCCCCTAAT 
CCCTCTGTCA AGTCACATGT TGCCAATTCT AACCCCCAAG CTAGCCGCAC CGCTAACCCT 
TCAGCCCTTG TACGTCCTGC TCACAATGGC TCATCCAAAC TTCAGCCCCC GCCTACATAT 
AAAGGTCACC ATCTTTCATT CTCACCGCAG GCCTCAAATG GCAGACCAGC TCTTCCCTCT 
ACTACTGCTT CTAGTTCTTC TCCCCCTTCT TCTGTCTCTC CCACTTCACT GACTCAAGGT 
GTGAAGAGCA TCCGCACAAT CCATACACCT AGCTTCACCA GCTACAAGTC ACAGAACGGA 
AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_002933147.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933193.2 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.

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CloneID	OXa12039
Clone ID Related Accession (Same CDS sequence)	XM_002933147.5 , XM_002933147.4
Accession Version	XM_002933147.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933147.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGTTTCA 
AAAGAGGGTT TAAATGCTGA AAAGCCAGAA GAAAAGTGGA AAATACAAAA TCCAGCATCT 
GAATCTGCAA AAATTAGGCA GGGAGATACT GTAATTGTGA ATGAGACCTT CAGTCTGTTT 
ACAGGAAGGG AGAAATATGG TGAGTGCCAA ACTGATAAAG AGATCTTACC TGCCCAGGAA 
TGTGAGAAAG GAGATGGGTC CCCACTTGTG AGCCCAGCAA TCCTTGAGAA GAGACTCATC 
AGTTTTAGCA CATCAAAAGA AACAGAGAGT GGGATCCCTC AACCCAAAAG AGAAGTGTTA 
AATGTCAAAG TTGGGGAAAT TTTGCATGTA GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC 
ATGACAGCTT TAAAGTCATT AAATGAGCAT GAAATGCCTT ATATGGACAT GGGCCAAACT 
GTTGTATTGA GACCTAAGTC ATCGAGAAGA AGTCTTAGTC AACAACAGTC TGATGACTCC 
AATTCTCCAA CTACGTCACC GACTGAAACA ACATCTCCAG CAGATAATAT TGCGTTCATG 
ATAACTAAAA CTAAAGTCCA GGTGCTTTCA ACTGGGGAGG TTCAGGACAT TGTCAGCCGT 
AAAGGAGAAG ATGTACAAAC CTTCAACATT GATAGAGGGA ATAACGAGAA AATACTCTCT 
GGGAATTTAG AAAACATTGG ATCTGAAGAC CCAGTGGTCT GCACTGACAA GAAACCAGTC 
ATCATCATAT TTGATGAACC AATGGATATT AAATCAGCAT ATAAGAGGCT TTCAACAATA 
TTTGAAGAAG GTGATGACGA TCTAGAAAAA CACATGTCAG AGGCTAAAAT AGACGAAGAA 
GATGAAGAAG AGGAAGCAGA AGTTCCTTCA CCCCAAGAAT CGAAAAAGGA GTTACCAATG 
GAACATACCA CAAAAGCATC CTCGGAGAAT CCAGGTTTGG AAAACAAGTA CAGATTCAAC 
TATCCAACTT TAGCCGAAAT GAAAAAGACC ATCTTCGAAG ACGCCAATGC CATACAGACC 
CATTTCACAG AGGAAGACAG GTCTGAAGCT TCCGATGATC AGCTTGGTCA AAGGCAGGAT 
GTCAAGAAAA AATTCAAGTT TAAATTTCCC AAAAAACAGC TCGCTGCGCT GACTCAAGCA 
ATCCGGACAG GGACAAAAAC TGGTAAGAAA ACATTACAGG TTGTGGTCTA CGAAGAGGAA 
GAAGAAGCAG ATGGGACTAT AAAACAACAC AAAGAAGCAA AGAGATTTGA AATTGCAAGA 
TCAAGTAGCA AAGAGGACAA TGCCAAATAC GGCGCAGAAG CAGTGGGACT TGAGACGGAC 
ATTCAGGCGT CACGGACAGA TGAGATTAAA AGAACCACCT ACAAGATTCT AGACAGTCTT 
GAGCAAACCA TAAAGCAGCA GGAAAACCTG ATGAGCGAAA TGGCACCCAT AATAGGGGCA 
GAGGCTTTAG AATCTAGAGA GAAATGTAAA GTCTCCAGTG ACATCAAGAC TGGTGCAGCG 
GAACCAGTGG AAGAACCAAA ACCTGTTGTA GAGACTCCTT CCTCCATCCC TACTGCAGCA 
CGTAAGGGTT CCAGTGGAGG AGCACAAACC AGCAGAATGC CAATCCCAAT GTCTTCTAAA 
AGTAGGCAAG CAAACGTGGA CAAAAGTAGC AAACAGCCCA AACTGCAGGA GCCGCAGCGC 
CAATACAGAC AGGCTAACGG AGGTACTAAG AAAGCTGGTG GGGATGTTAA GGCTTCTGCC 
CCTTCTTTAT CTGTTTCTAA GATTCCAGGC CTTTCCTCTA ACACTGGGAA AAGTGGCTCT 
GTGCCTGCTC AGTCTAGCGA GACCTCTAAC CCCCCTAATC CCTCTGTCAA GTCACATGTT 
GCCAATTCTA ACCCCCAAGC TAGCCGCACC GCTAACCCTT CAGCCCTTGT ACGTCCTGCT 
CACAATGGCT CATCCAAACT TCAGCCCCCG CCTACATATA AAGGTCACCA TCTTTCATTC 
TCACCGCAGG CCTCAAATGG CAGACCAGCT CTTCCCTCTA CTACTGCTTC TAGTTCTTCT 
CCCCCTTCTT CTGTCTCTCC CACTTCACTG ACTCAAGGTG TGAAGAGCAT CCGCACAATC 
CATACACCTA GCTTCACCAG CTACAAGTCA CAGAACGGAA GTGCAAGCAA AGGCCTGAAT 
GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933193.2
CDS	315..6152
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_002933147.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933147.5

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGTTTCA 
AAAGAGGGTT TAAATGCTGA AAAGCCAGAA GAAAAGTGGA AAATACAAAA TCCAGCATCT 
GAATCTGCAA AAATTAGGCA GGGAGATACT GTAATTGTGA ATGAGACCTT CAGTCTGTTT 
ACAGGAAGGG AGAAATATGG TGAGTGCCAA ACTGATAAAG AGATCTTACC TGCCCAGGAA 
TGTGAGAAAG GAGATGGGTC CCCACTTGTG AGCCCAGCAA TCCTTGAGAA GAGACTCATC 
AGTTTTAGCA CATCAAAAGA AACAGAGAGT GGGATCCCTC AACCCAAAAG AGAAGTGTTA 
AATGTCAAAG TTGGGGAAAT TTTGCATGTA GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC 
ATGACAGCTT TAAAGTCATT AAATGAGCAT GAAATGCCTT ATATGGACAT GGGCCAAACT 
GTTGTATTGA GACCTAAGTC ATCGAGAAGA AGTCTTAGTC AACAACAGTC TGATGACTCC 
AATTCTCCAA CTACGTCACC GACTGAAACA ACATCTCCAG CAGATAATAT TGCGTTCATG 
ATAACTAAAA CTAAAGTCCA GGTGCTTTCA ACTGGGGAGG TTCAGGACAT TGTCAGCCGT 
AAAGGAGAAG ATGTACAAAC CTTCAACATT GATAGAGGGA ATAACGAGAA AATACTCTCT 
GGGAATTTAG AAAACATTGG ATCTGAAGAC CCAGTGGTCT GCACTGACAA GAAACCAGTC 
ATCATCATAT TTGATGAACC AATGGATATT AAATCAGCAT ATAAGAGGCT TTCAACAATA 
TTTGAAGAAG GTGATGACGA TCTAGAAAAA CACATGTCAG AGGCTAAAAT AGACGAAGAA 
GATGAAGAAG AGGAAGCAGA AGTTCCTTCA CCCCAAGAAT CGAAAAAGGA GTTACCAATG 
GAACATACCA CAAAAGCATC CTCGGAGAAT CCAGGTTTGG AAAACAAGTA CAGATTCAAC 
TATCCAACTT TAGCCGAAAT GAAAAAGACC ATCTTCGAAG ACGCCAATGC CATACAGACC 
CATTTCACAG AGGAAGACAG GTCTGAAGCT TCCGATGATC AGCTTGGTCA AAGGCAGGAT 
GTCAAGAAAA AATTCAAGTT TAAATTTCCC AAAAAACAGC TCGCTGCGCT GACTCAAGCA 
ATCCGGACAG GGACAAAAAC TGGTAAGAAA ACATTACAGG TTGTGGTCTA CGAAGAGGAA 
GAAGAAGCAG ATGGGACTAT AAAACAACAC AAAGAAGCAA AGAGATTTGA AATTGCAAGA 
TCAAGTAGCA AAGAGGACAA TGCCAAATAC GGCGCAGAAG CAGTGGGACT TGAGACGGAC 
ATTCAGGCGT CACGGACAGA TGAGATTAAA AGAACCACCT ACAAGATTCT AGACAGTCTT 
GAGCAAACCA TAAAGCAGCA GGAAAACCTG ATGAGCGAAA TGGCACCCAT AATAGGGGCA 
GAGGCTTTAG AATCTAGAGA GAAATGTAAA GTCTCCAGTG ACATCAAGAC TGGTGCAGCG 
GAACCAGTGG AAGAACCAAA ACCTGTTGTA GAGACTCCTT CCTCCATCCC TACTGCAGCA 
CGTAAGGGTT CCAGTGGAGG AGCACAAACC AGCAGAATGC CAATCCCAAT GTCTTCTAAA 
AGTAGGCAAG CAAACGTGGA CAAAAGTAGC AAACAGCCCA AACTGCAGGA GCCGCAGCGC 
CAATACAGAC AGGCTAACGG AGGTACTAAG AAAGCTGGTG GGGATGTTAA GGCTTCTGCC 
CCTTCTTTAT CTGTTTCTAA GATTCCAGGC CTTTCCTCTA ACACTGGGAA AAGTGGCTCT 
GTGCCTGCTC AGTCTAGCGA GACCTCTAAC CCCCCTAATC CCTCTGTCAA GTCACATGTT 
GCCAATTCTA ACCCCCAAGC TAGCCGCACC GCTAACCCTT CAGCCCTTGT ACGTCCTGCT 
CACAATGGCT CATCCAAACT TCAGCCCCCG CCTACATATA AAGGTCACCA TCTTTCATTC 
TCACCGCAGG CCTCAAATGG CAGACCAGCT CTTCCCTCTA CTACTGCTTC TAGTTCTTCT 
CCCCCTTCTT CTGTCTCTCC CACTTCACTG ACTCAAGGTG TGAAGAGCAT CCGCACAATC 
CATACACCTA GCTTCACCAG CTACAAGTCA CAGAACGGAA GTGCAAGCAA AGGCCTGAAT 
GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915436.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915493.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.

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CloneID	OXa12040
Clone ID Related Accession (Same CDS sequence)	XM_004915436.4 , XM_004915436.3
Accession Version	XM_004915436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915436.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGTTTCAAAA GAGGGTTTAA ATGCTGAAAA GCCAGAAGAA 
AAGTGGAAAA TACAAAATCC AGCATCTGAA TCTGCAAAAA TTAGGCAGGG AGATACTGTA 
ATTGTGAATG AGACCTTCAG TCTGTTTACA GGAAGGGAGA AATATGGTGA GTGCCAAACT 
GATAAAGAGA TCTTACCTGC CCAGGAATGT GAGAAAGGAG ATGGGTCCCC ACTTGTGAGC 
CCAGCAATCC TTGAGAAGAG ACTCATCAGT TTTAGCACAT CAAAAGAAAC AGAGAGTGGG 
ATCCCTCAAC CCAAAAGAGA AGTGTTAAAT GTCAAAGTTG GGGAAATTTT GCATGTAGAA 
AAGAAGGAAG AGGTGCTAGA AAGTCACATG ACAGCTTTAA AGTCATTAAA TGAGCATGAA 
ATGCCTTATA TGGACATGGG CCAAACTGTT GTATTGAGAC CTAAGTCATC GAGAAGAAGT 
CTTAGTCAAC AACAGTCTGA TGACTCCAAT TCTCCAACTA CGTCACCGAC TGAAACAACA 
TCTCCAGCAG ATAATATTGC GTTCATGATA ACTAAAACTA AAGTCCAGGT GCTTTCAACT 
GGGGAGGTTC AGGACATTGT CAGCCGTAAA GGAGAAGATG TACAAACCTT CAACATTGAT 
AGAGGGAATA ACGAGAAAAT ACTCTCTGGG AATTTAGAAA ACATTGGATC TGAAGACCCA 
GTGGTCTGCA CTGACAAGAA ACCAGTCATC ATCATATTTG ATGAACCAAT GGATATTAAA 
TCAGCATATA AGAGGCTTTC AACAATATTT GAAGAAGGTG ATGACGATCT AGAAAAACAC 
ATGTCAGAGG CTAAAATAGA CGAAGAAGAT GAAGAAGAGG AAGCAGAAGT TCCTTCACCC 
CAAGAATCGA AAAAGGAGTT ACCAATGGAA CATACCACAA AAGCATCCTC GGAGAATCCA 
GGTTTGGAAA ACAAGTACAG ATTCAACTAT CCAACTTTAG CCGAAATGAA AAAGACCATC 
TTCGAAGACG CCAATGCCAT ACAGACCCAT TTCACAGAGG AAGACAGGTC TGAAGCTTCC 
GATGATCAGC TTGGTCAAAG GCAGGATGTC AAGAAAAAAT TCAAGTTTAA ATTTCCCAAA 
AAACAGCTCG CTGCGCTGAC TCAAGCAATC CGGACAGGGA CAAAAACTGG TAAGAAAACA 
TTACAGGTTG TGGTCTACGA AGAGGAAGAA GAAGCAGATG GGACTATAAA ACAACACAAA 
GAAGCAAAGA GATTTGAAAT TGCAAGATCA AGTAGCAAAG AGGACAATGC CAAATACGGC 
GCAGAAGCAG TGGGACTTGA GACGGACATT CAGGCGTCAC GGACAGATGA GATTAAAAGA 
ACCACCTACA AGATTCTAGA CAGTCTTGAG CAAACCATAA AGCAGCAGGA AAACCTGATG 
AGCGAAATGG CACCCATAAT AGGGGCAGAG GCTTTAGAAT CTAGAGAGAA ATGTAAAGTC 
TCCAGTGACA TCAAGACTGG TGCAGCGGAA CCAGTGGAAG AACCAAAACC TGTTGTAGAG 
ACTCCTTCCT CCATCCCTAC TGCAGCACGT AAGGGTTCCA GTGGAGGAGC ACAAACCAGC 
AGAATGCCAA TCCCAATGTC TTCTAAAAGT AGGCAAGCAA ACGTGGACAA AAGTAGCAAA 
CAGCCCAAAC TGCAGGAGCC GCAGCGCCAA TACAGACAGG CTAACGGAGG TACTAAGAAA 
GCTGGTGGGG ATGTTAAGGC TTCTGCCCCT TCTTTATCTG TTTCTAAGAT TCCAGGCCTT 
TCCTCTAACA CTGGGAAAAG TGGCTCTGTG CCTGCTCAGT CTAGCGAGAC CTCTAACCCC 
CCTAATCCCT CTGTCAAGTC ACATGTTGCC AATTCTAACC CCCAAGCTAG CCGCACCGCT 
AACCCTTCAG CCCTTGTACG TCCTGCTCAC AATGGCTCAT CCAAACTTCA GCCCCCGCCT 
ACATATAAAG GTCACCATCT TTCATTCTCA CCGCAGGCCT CAAATGGCAG ACCAGCTCTT 
CCCTCTACTA CTGCTTCTAG TTCTTCTCCC CCTTCTTCTG TCTCTCCCAC TTCACTGACT 
CAAGGTGTGA AGAGCATCCG CACAATCCAT ACACCTAGCT TCACCAGCTA CAAGTCACAG 
AACGGAAGTG CAAGCAAAGG CCTGAATGCC AAGGAGACCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915493.1
CDS	315..6119
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915436.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915436.4

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGTTTCAAAA GAGGGTTTAA ATGCTGAAAA GCCAGAAGAA 
AAGTGGAAAA TACAAAATCC AGCATCTGAA TCTGCAAAAA TTAGGCAGGG AGATACTGTA 
ATTGTGAATG AGACCTTCAG TCTGTTTACA GGAAGGGAGA AATATGGTGA GTGCCAAACT 
GATAAAGAGA TCTTACCTGC CCAGGAATGT GAGAAAGGAG ATGGGTCCCC ACTTGTGAGC 
CCAGCAATCC TTGAGAAGAG ACTCATCAGT TTTAGCACAT CAAAAGAAAC AGAGAGTGGG 
ATCCCTCAAC CCAAAAGAGA AGTGTTAAAT GTCAAAGTTG GGGAAATTTT GCATGTAGAA 
AAGAAGGAAG AGGTGCTAGA AAGTCACATG ACAGCTTTAA AGTCATTAAA TGAGCATGAA 
ATGCCTTATA TGGACATGGG CCAAACTGTT GTATTGAGAC CTAAGTCATC GAGAAGAAGT 
CTTAGTCAAC AACAGTCTGA TGACTCCAAT TCTCCAACTA CGTCACCGAC TGAAACAACA 
TCTCCAGCAG ATAATATTGC GTTCATGATA ACTAAAACTA AAGTCCAGGT GCTTTCAACT 
GGGGAGGTTC AGGACATTGT CAGCCGTAAA GGAGAAGATG TACAAACCTT CAACATTGAT 
AGAGGGAATA ACGAGAAAAT ACTCTCTGGG AATTTAGAAA ACATTGGATC TGAAGACCCA 
GTGGTCTGCA CTGACAAGAA ACCAGTCATC ATCATATTTG ATGAACCAAT GGATATTAAA 
TCAGCATATA AGAGGCTTTC AACAATATTT GAAGAAGGTG ATGACGATCT AGAAAAACAC 
ATGTCAGAGG CTAAAATAGA CGAAGAAGAT GAAGAAGAGG AAGCAGAAGT TCCTTCACCC 
CAAGAATCGA AAAAGGAGTT ACCAATGGAA CATACCACAA AAGCATCCTC GGAGAATCCA 
GGTTTGGAAA ACAAGTACAG ATTCAACTAT CCAACTTTAG CCGAAATGAA AAAGACCATC 
TTCGAAGACG CCAATGCCAT ACAGACCCAT TTCACAGAGG AAGACAGGTC TGAAGCTTCC 
GATGATCAGC TTGGTCAAAG GCAGGATGTC AAGAAAAAAT TCAAGTTTAA ATTTCCCAAA 
AAACAGCTCG CTGCGCTGAC TCAAGCAATC CGGACAGGGA CAAAAACTGG TAAGAAAACA 
TTACAGGTTG TGGTCTACGA AGAGGAAGAA GAAGCAGATG GGACTATAAA ACAACACAAA 
GAAGCAAAGA GATTTGAAAT TGCAAGATCA AGTAGCAAAG AGGACAATGC CAAATACGGC 
GCAGAAGCAG TGGGACTTGA GACGGACATT CAGGCGTCAC GGACAGATGA GATTAAAAGA 
ACCACCTACA AGATTCTAGA CAGTCTTGAG CAAACCATAA AGCAGCAGGA AAACCTGATG 
AGCGAAATGG CACCCATAAT AGGGGCAGAG GCTTTAGAAT CTAGAGAGAA ATGTAAAGTC 
TCCAGTGACA TCAAGACTGG TGCAGCGGAA CCAGTGGAAG AACCAAAACC TGTTGTAGAG 
ACTCCTTCCT CCATCCCTAC TGCAGCACGT AAGGGTTCCA GTGGAGGAGC ACAAACCAGC 
AGAATGCCAA TCCCAATGTC TTCTAAAAGT AGGCAAGCAA ACGTGGACAA AAGTAGCAAA 
CAGCCCAAAC TGCAGGAGCC GCAGCGCCAA TACAGACAGG CTAACGGAGG TACTAAGAAA 
GCTGGTGGGG ATGTTAAGGC TTCTGCCCCT TCTTTATCTG TTTCTAAGAT TCCAGGCCTT 
TCCTCTAACA CTGGGAAAAG TGGCTCTGTG CCTGCTCAGT CTAGCGAGAC CTCTAACCCC 
CCTAATCCCT CTGTCAAGTC ACATGTTGCC AATTCTAACC CCCAAGCTAG CCGCACCGCT 
AACCCTTCAG CCCTTGTACG TCCTGCTCAC AATGGCTCAT CCAAACTTCA GCCCCCGCCT 
ACATATAAAG GTCACCATCT TTCATTCTCA CCGCAGGCCT CAAATGGCAG ACCAGCTCTT 
CCCTCTACTA CTGCTTCTAG TTCTTCTCCC CCTTCTTCTG TCTCTCCCAC TTCACTGACT 
CAAGGTGTGA AGAGCATCCG CACAATCCAT ACACCTAGCT TCACCAGCTA CAAGTCACAG 
AACGGAAGTG CAAGCAAAGG CCTGAATGCC AAGGAGACCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915438.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915495.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.

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CloneID	OXa12042
Clone ID Related Accession (Same CDS sequence)	XM_004915438.4 , XM_004915438.3
Accession Version	XM_004915438.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915438.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGACAAAA 
AGTCCGAAGC TGTCTTCAAG CCCACAGCCG AGCGTAGGAG ACCCGGCAGA ACATCTGTCA 
GAGGCGTCTG CTGATTCCGG AGACATGATG TTCGAAGCGG ATTCGGCAGT GCCGTTTGTC 
CGAGGCAGTC GCACAAGAGC CAGCCTTCCG GTGGTCAGAT CCACAAATCA AACGAAAGAG 
AGATCACTTG GTACGCTCTT CCTACAGTAT GGCGACGATA CCAAGAAAAT GCAGATGCCC 
AATGAAATCA CAAGCGTAGA CACGATCCGT GCCCTGTTCG TAAGTGCCTT CCCCCAGCAA 
CTGACAATGA AGATCCTGGA ATCGCCCAGC ACAGCCATTT ATATTAAGGA TGAGGGCCGA 
AATGTCTATT ATGAGCTCAG CGATGTCAGG AACATACAAG ACAGATGCTT CCTCAAAGTC 
TACAATAAAG ACCCTGCGCA TGCATTTAAC CACACTCGTG GGGCGGTCAA TGGAGATGTA 
AGGATGCAAC GAGATATTTC CTACTCGGTT CGGGAAGGCC CGGCGATCCA TAGGCCTGGC 
TCTGCTACAT ACCCCTCCCA TGCTGGCCCA GTGTCACCCC CTCCCACACC TGTTCCCCAC 
TCCATGCCAT CATCTCCATC AAGAATCCCC TATGGCGGTG GGAGGCCTGC AGTGGTACAG 
AGCAACACCA TGCAGAGGGA AAGAATACCA AGCGTGCCGG CACCTAGATC CATATCTCCT 
AGCCCCAGTG CTATTTTGGA AAGGAGAGAC GTAAAACCAG ATGAGGACAT GGGCAATAAA 
AATGTCCAGC TGCTAAGGAA TGACAGCATT TACCAAGACC CTTATTTATA CCATGAAGGA 
CGGATGAGTA TGGCATCATC ACATGGTGGG CACCCTCTCG ACGTCCCTGA TCATATTATT 
GCATACCACA GAGGGTCCTT GAGATCTGCA AGCACTTATT CTAATTCTTC TATGCAAATG 
GAGATGATGG AACAATCCAT GTACAGACAA AAAAGCCGTA AGCACGTAGA GAGTCACTTA 
CCGACACTTG GTTCTAAAAC TCCCCCAACC TCTCCTCACA GAGTGGCTGA TATAAGGATG 
ATGGAGATGC ATGCCCATAA TGCCCATCTG CCTCCCCACG GCATGCAGGC CGACAGATCT 
TCTCCTCTCC GTCAGTCATT TAAAAAGGAG CAAGGGCCTG GCATGTTTGT AGAAGCTAAA 
GTGCGAAACA CAGGTGGAAT AATGGGCATG GCGGACATTG TCCCATCTCC TACCGATAAG 
CAAGTATTTG GAGGGTATGG AGTAGCTGTG CCACCAAAAG ACCCCTATAC AAGAGAGAGG 
ATGCATGCCA TGGAGAAACA GATTGCCAGT TTAACTGGTC TTGTTCAGAC TGCACTTTTG 
AAAGGGCCCA GCCCAAACAA CTCTGCACCT AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915495.1
CDS	413..5872
Translation	MQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915438.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915438.4

ORF Insert Sequence:

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGACAAAA 
AGTCCGAAGC TGTCTTCAAG CCCACAGCCG AGCGTAGGAG ACCCGGCAGA ACATCTGTCA 
GAGGCGTCTG CTGATTCCGG AGACATGATG TTCGAAGCGG ATTCGGCAGT GCCGTTTGTC 
CGAGGCAGTC GCACAAGAGC CAGCCTTCCG GTGGTCAGAT CCACAAATCA AACGAAAGAG 
AGATCACTTG GTACGCTCTT CCTACAGTAT GGCGACGATA CCAAGAAAAT GCAGATGCCC 
AATGAAATCA CAAGCGTAGA CACGATCCGT GCCCTGTTCG TAAGTGCCTT CCCCCAGCAA 
CTGACAATGA AGATCCTGGA ATCGCCCAGC ACAGCCATTT ATATTAAGGA TGAGGGCCGA 
AATGTCTATT ATGAGCTCAG CGATGTCAGG AACATACAAG ACAGATGCTT CCTCAAAGTC 
TACAATAAAG ACCCTGCGCA TGCATTTAAC CACACTCGTG GGGCGGTCAA TGGAGATGTA 
AGGATGCAAC GAGATATTTC CTACTCGGTT CGGGAAGGCC CGGCGATCCA TAGGCCTGGC 
TCTGCTACAT ACCCCTCCCA TGCTGGCCCA GTGTCACCCC CTCCCACACC TGTTCCCCAC 
TCCATGCCAT CATCTCCATC AAGAATCCCC TATGGCGGTG GGAGGCCTGC AGTGGTACAG 
AGCAACACCA TGCAGAGGGA AAGAATACCA AGCGTGCCGG CACCTAGATC CATATCTCCT 
AGCCCCAGTG CTATTTTGGA AAGGAGAGAC GTAAAACCAG ATGAGGACAT GGGCAATAAA 
AATGTCCAGC TGCTAAGGAA TGACAGCATT TACCAAGACC CTTATTTATA CCATGAAGGA 
CGGATGAGTA TGGCATCATC ACATGGTGGG CACCCTCTCG ACGTCCCTGA TCATATTATT 
GCATACCACA GAGGGTCCTT GAGATCTGCA AGCACTTATT CTAATTCTTC TATGCAAATG 
GAGATGATGG AACAATCCAT GTACAGACAA AAAAGCCGTA AGCACGTAGA GAGTCACTTA 
CCGACACTTG GTTCTAAAAC TCCCCCAACC TCTCCTCACA GAGTGGCTGA TATAAGGATG 
ATGGAGATGC ATGCCCATAA TGCCCATCTG CCTCCCCACG GCATGCAGGC CGACAGATCT 
TCTCCTCTCC GTCAGTCATT TAAAAAGGAG CAAGGGCCTG GCATGTTTGT AGAAGCTAAA 
GTGCGAAACA CAGGTGGAAT AATGGGCATG GCGGACATTG TCCCATCTCC TACCGATAAG 
CAAGTATTTG GAGGGTATGG AGTAGCTGTG CCACCAAAAG ACCCCTATAC AAGAGAGAGG 
ATGCATGCCA TGGAGAAACA GATTGCCAGT TTAACTGGTC TTGTTCAGAC TGCACTTTTG 
AAAGGGCCCA GCCCAAACAA CTCTGCACCT AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915439.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915496.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.

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CloneID	OXa12043
Clone ID Related Accession (Same CDS sequence)	XM_004915439.3 , XM_004915439.4
Accession Version	XM_004915439.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915439.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAC AAGCTGCCCC TAAACTCCAA GAGGAGAAGG CTGAAAAGGG ATTTGTGTCA 
CTGTGCTGTG CAGCCCCCAG GCATCCATCA AGAATAAGCA AGACAAAAAG TCCGAAGCTG 
TCTTCAAGCC CACAGCCGAG CGTAGGAGAC CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT 
GATTCCGGAG ACATGATGTT CGAAGCGGAT TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC 
ACAAGAGCCA GCCTTCCGGT GGTCAGATCC ACAAATCAAA CGAAAGAGAG ATCACTTGGT 
ACGCTCTTCC TACAGTATGG CGACGATACC AAGAAAATGC AGATGCCCAA TGAAATCACA 
AGCGTAGACA CGATCCGTGC CCTGTTCGTA AGTGCCTTCC CCCAGCAACT GACAATGAAG 
ATCCTGGAAT CGCCCAGCAC AGCCATTTAT ATTAAGGATG AGGGCCGAAA TGTCTATTAT 
GAGCTCAGCG ATGTCAGGAA CATACAAGAC AGATGCTTCC TCAAAGTCTA CAATAAAGAC 
CCTGCGCATG CATTTAACCA CACTCGTGGG GCGGTCAATG GAGATGTAAG GATGCAACGA 
GATATTTCCT ACTCGGTTCG GGAAGGCCCG GCGATCCATA GGCCTGGCTC TGCTACATAC 
CCCTCCCATG CTGGCCCAGT GTCACCCCCT CCCACACCTG TTCCCCACTC CATGCCATCA 
TCTCCATCAA GAATCCCCTA TGGCGGTGGG AGGCCTGCAG TGGTACAGAG CAACACCATG 
CAGAGGGAAA GAATACCAAG CGTGCCGGCA CCTAGATCCA TATCTCCTAG CCCCAGTGCT 
ATTTTGGAAA GGAGAGACGT AAAACCAGAT GAGGACATGG GCAATAAAAA TGTCCAGCTG 
CTAAGGAATG ACAGCATTTA CCAAGACCCT TATTTATACC ATGAAGGACG GATGAGTATG 
GCATCATCAC ATGGTGGGCA CCCTCTCGAC GTCCCTGATC ATATTATTGC ATACCACAGA 
GGGTCCTTGA GATCTGCAAG CACTTATTCT AATTCTTCTA TGCAAATGGA GATGATGGAA 
CAATCCATGT ACAGACAAAA AAGCCGTAAG CACGTAGAGA GTCACTTACC GACACTTGGT 
TCTAAAACTC CCCCAACCTC TCCTCACAGA GTGGCTGATA TAAGGATGAT GGAGATGCAT 
GCCCATAATG CCCATCTGCC TCCCCACGGC ATGCAGGCCG ACAGATCTTC TCCTCTCCGT 
CAGTCATTTA AAAAGGAGCA AGGGCCTGGC ATGTTTGTAG AAGCTAAAGT GCGAAACACA 
GGTGGAATAA TGGGCATGGC GGACATTGTC CCATCTCCTA CCGATAAGCA AGTATTTGGA 
GGGTATGGAG TAGCTGTGCC ACCAAAAGAC CCCTATACAA GAGAGAGGAT GCATGCCATG 
GAGAAACAGA TTGCCAGTTT AACTGGTCTT GTTCAGACTG CACTTTTGAA AGGGCCCAGC 
CCAAACAACT CTGCACCTAG CGACAGAGTC TTAAAATCAG CCAACGGCAG CGCTTCAAGC 
TCAGATCGGC CAGGAAATCA AGGTGTTGCT GCTGCAAAGA ATTCATTGGC CATTTCAGAA 
ACAATAACTG TTATCAACCA GCAGTCGTCT ACCTGCTCGC AGGAAATGCA CGTCAGCCTA 
CAGGACATGA GGCGCAATGT GGCCGAACTT CGTCTTCAGC TGCATAAGAT GAGACAACTG 
CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG ATGATGAAGA AAGCGGAGAT GGAAATCAGC 
AGCAAAGTGA CTGAGATGGT CAAGGGCCTG GAGGACCCGG TGCAGAGACA GCGCGTCTTG 
GTGGAACAAG AGCGCCAGAA GTACCTCGGT GACGAGGAAC AAATTGTTGC AAAGTTGTGT 
GACCTTGAAG ACTTTGTGGA ATCCCTTAAA AAGGATCAGA GCACATCCCA GAGGTCGATC 
ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC ATGCTGCGCC AGATAGGAGA GGCTGTTTCC 
AACCTGAAAG GAGAGTTTCC GTACCTCCAG AATAAGATGC GGGCCGTCTT GCGTGTGGAG 
GTGGAGGCCG TCAGATTTCT GAAAGAGGAG CCCCACAAAC TGGACAGCTT ACTGAAAAGG 
ATCCGAAACA TGACAGATGC CCTTTCTGCC TTAAGAAGAC ACGCCACTGA CGGATCCTCC 
AAATCCATTG ACAACAATCA GACCAGCCAG GTTCTAATAA CAGAAAGAGT AACTGAGACT 
GATTCACTCC AGGTCCATGA GGAAAAGTCC CAAGTGTACC TTCAGCCAAC ACAAACTGAT 
GGAGGAGCAG CAGTGGAATC GCCGACCACA TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG 
GCCGGCCTCA GAGTCCATCA AGTTCAAAGT TCTCCGGTTC TCATGCAGCA GTCCCAGCAC 
TCGTCTGCAC TCGTGAACCA CACTCCTGCC CCCAGTACCC AGAGCAGTCA CAGCACAGAA 
TTACTAGTGA GCCATCAAAC TGCCAGCGTG CCACCCAGCG TCACCCCAGA AAGTGCAAGG 
AGGAACCCAC CGACGGCCGC ACAGGAAGGA TCTTCCAAGC AAAGTCTTTT TATTGAGGAA 
ATGAGTCAAG TGACTTACAG GAACCGAGCG GTGTCCATTG AGGAGGCAGA GAGAAAATTT 
GAAGAGAAGA GACAGAATCT TGATCATTAT AACGGAAAGG AATTTGAGAA AATGCTGGAA 
GAAGCACAAG CCAGTATCAT GAAGGCCATT CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG 
AAAGTGGAGA TTGTGGACAA AAAAGAGGTC ACGGAGGTAA AAACCATAAT GGAGCAGGAT 
AACGATAAAG TAACAAAGTC TCCCCCTCCA CCACCACCTC GTCGTATGTA CCCACCAGGA 
TCTGGACTGA ACAGTGTATG GACAGCAGAA TCAACGTTCT TGGTCAGGAA GGACAGCAGC 
AAGGATTCGG AGGAAGCCCT TCCTAAGGTG CCATCAAAGG CAGAAACTGA AGAGCCTCAT 
CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT TCTGCCGTAA AGGATGAGGA TGAAGAAGAG 
GAAGGAGCAA GGATTATGGC TGAACTTCAG GCCTTCCAGA AGTGTTCTTT TGTGGATGTA 
AATCCTAAAA GCCCAAATGA GCTTTCTAAA GCAGATAGCC AAGATTACAG AGCTACAGCT 
GTAACCCAAG CTAAAGAGAA GAAGGTTTCA AAAGAGGGTT TAAATGCTGA AAAGCCAGAA 
GAAAAGTGGA AAATACAAAA TCCAGCATCT GAATCTGCAA AAATTAGGCA GGGAGATACT 
GTAATTGTGA ATGAGACCTT CAGTCTGTTT ACAGGAAGGG AGAAATATGG TGAGTGCCAA 
ACTGATAAAG AGATCTTACC TGCCCAGGAA TGTGAGAAAG GAGATGGGTC CCCACTTGTG 
AGCCCAGCAA TCCTTGAGAA GAGACTCATC AGTTTTAGCA CATCAAAAGA AACAGAGAGT 
GGGATCCCTC AACCCAAAAG AGAAGTGTTA AATGTCAAAG TTGGGGAAAT TTTGCATGTA 
GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC ATGACAGCTT TAAAGTCATT AAATGAGCAT 
GAAATGCCTT ATATGGACAT GGGCCAAACT GTTGTATTGA GACCTAAGTC ATCGAGAAGA 
AGTCTTAGTC AACAACAGTC TGATGACTCC AATTCTCCAA CTACGTCACC GACTGAAACA 
ACATCTCCAG CAGATAATAT TGCGTTCATG ATAACTAAAA CTAAAGTCCA GGTGCTTTCA 
ACTGGGGAGG TTCAGGACAT TGTCAGCCGT AAAGGAGAAG ATGTACAAAC CTTCAACATT 
GATAGAGGGA ATAACGAGAA AATACTCTCT GGGAATTTAG AAAACATTGG ATCTGAAGAC 
CCAGTGGTCT GCACTGACAA GAAACCAGTC ATCATCATAT TTGATGAACC AATGGATATT 
AAATCAGCAT ATAAGAGGCT TTCAACAATA TTTGAAGAAG GTGATGACGA TCTAGAAAAA 
CACATGTCAG AGGCTAAAAT AGACGAAGAA GATGAAGAAG AGGAAGCAGA AGTTCCTTCA 
CCCCAAGAAT CGAAAAAGGA GTTACCAATG GAACATACCA CAAAAGCATC CTCGGAGAAT 
CCAGGTTTGG AAAACAAGTA CAGATTCAAC TATCCAACTT TAGCCGAAAT GAAAAAGACC 
ATCTTCGAAG ACGCCAATGC CATACAGACC CATTTCACAG AGGAAGACAG GTCTGAAGCT 
TCCGATGATC AGCTTGGTCA AAGGCAGGAT GTCAAGAAAA AATTCAAGTT TAAATTTCCC 
AAAAAACAGC TCGCTGCGCT GACTCAAGCA ATCCGGACAG GGACAAAAAC TGGTAAGAAA 
ACATTACAGG TTGTGGTCTA CGAAGAGGAA GAAGAAGCAG ATGGGACTAT AAAACAACAC 
AAAGAAGCAA AGAGATTTGA AATTGCAAGA TCAAGTAGCA AAGAGGACAA TGCCAAATAC 
GGCGCAGAAG CAGTGGGACT TGAGACGGAC ATTCAGGCGT CACGGACAGA TGAGATTAAA 
AGAACCACCT ACAAGATTCT AGACAGTCTT GAGCAAACCA TAAAGCAGCA GGAAAACCTG 
ATGAGCGAAA TGGCACCCAT AATAGGGGCA GAGGCTTTAG AATCTAGAGA GAAATGTAAA 
GTCTCCAGTG ACATCAAGAC TGGTGCAGCG GAACCAGTGG AAGAACCAAA ACCTGTTGTA 
GAGACTCCTT CCTCCATCCC TACTGCAGCA CGTAAGGGTT CCAGTGGAGG AGCACAAACC 
AGCAGAATGC CAATCCCAAT GTCTTCTAAA AGTAGGCAAG CAAACGTGGA CAAAAGTAGC 
AAACAGCCCA AACTGCAGGA GCCGCAGCGC CAATACAGAC AGGCTAACGG AGGTACTAAG 
AAAGCTGGTG GGGATGTTAA GGCTTCTGCC CCTTCTTTAT CTGTTTCTAA GATTCCAGGC 
CTTTCCTCTA ACACTGGGAA AAGTGGCTCT GTGCCTGCTC AGTCTAGCGA GACCTCTAAC 
CCCCCTAATC CCTCTGTCAA GTCACATGTT GCCAATTCTA ACCCCCAAGC TAGCCGCACC 
GCTAACCCTT CAGCCCTTGT ACGTCCTGCT CACAATGGCT CATCCAAACT TCAGCCCCCG 
CCTACATATA AAGGTCACCA TCTTTCATTC TCACCGCAGG CCTCAAATGG CAGACCAGCT 
CTTCCCTCTA CTACTGCTTC TAGTTCTTCT CCCCCTTCTT CTGTCTCTCC CACTTCACTG 
ACTCAAGGTG TGAAGAGCAT CCGCACAATC CATACACCTA GCTTCACCAG CTACAAGTCA 
CAGAACGGAA GTGCAAGCAA AGGCCTGAAT GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915496.1
CDS	129..5576
Translation	MEQQAAPKLQEEKAEKGFVSLCCAAPRHPSRISKTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915439.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915439.4

ORF Insert Sequence:

ATGGAACAAC AAGCTGCCCC TAAACTCCAA GAGGAGAAGG CTGAAAAGGG ATTTGTGTCA 
CTGTGCTGTG CAGCCCCCAG GCATCCATCA AGAATAAGCA AGACAAAAAG TCCGAAGCTG 
TCTTCAAGCC CACAGCCGAG CGTAGGAGAC CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT 
GATTCCGGAG ACATGATGTT CGAAGCGGAT TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC 
ACAAGAGCCA GCCTTCCGGT GGTCAGATCC ACAAATCAAA CGAAAGAGAG ATCACTTGGT 
ACGCTCTTCC TACAGTATGG CGACGATACC AAGAAAATGC AGATGCCCAA TGAAATCACA 
AGCGTAGACA CGATCCGTGC CCTGTTCGTA AGTGCCTTCC CCCAGCAACT GACAATGAAG 
ATCCTGGAAT CGCCCAGCAC AGCCATTTAT ATTAAGGATG AGGGCCGAAA TGTCTATTAT 
GAGCTCAGCG ATGTCAGGAA CATACAAGAC AGATGCTTCC TCAAAGTCTA CAATAAAGAC 
CCTGCGCATG CATTTAACCA CACTCGTGGG GCGGTCAATG GAGATGTAAG GATGCAACGA 
GATATTTCCT ACTCGGTTCG GGAAGGCCCG GCGATCCATA GGCCTGGCTC TGCTACATAC 
CCCTCCCATG CTGGCCCAGT GTCACCCCCT CCCACACCTG TTCCCCACTC CATGCCATCA 
TCTCCATCAA GAATCCCCTA TGGCGGTGGG AGGCCTGCAG TGGTACAGAG CAACACCATG 
CAGAGGGAAA GAATACCAAG CGTGCCGGCA CCTAGATCCA TATCTCCTAG CCCCAGTGCT 
ATTTTGGAAA GGAGAGACGT AAAACCAGAT GAGGACATGG GCAATAAAAA TGTCCAGCTG 
CTAAGGAATG ACAGCATTTA CCAAGACCCT TATTTATACC ATGAAGGACG GATGAGTATG 
GCATCATCAC ATGGTGGGCA CCCTCTCGAC GTCCCTGATC ATATTATTGC ATACCACAGA 
GGGTCCTTGA GATCTGCAAG CACTTATTCT AATTCTTCTA TGCAAATGGA GATGATGGAA 
CAATCCATGT ACAGACAAAA AAGCCGTAAG CACGTAGAGA GTCACTTACC GACACTTGGT 
TCTAAAACTC CCCCAACCTC TCCTCACAGA GTGGCTGATA TAAGGATGAT GGAGATGCAT 
GCCCATAATG CCCATCTGCC TCCCCACGGC ATGCAGGCCG ACAGATCTTC TCCTCTCCGT 
CAGTCATTTA AAAAGGAGCA AGGGCCTGGC ATGTTTGTAG AAGCTAAAGT GCGAAACACA 
GGTGGAATAA TGGGCATGGC GGACATTGTC CCATCTCCTA CCGATAAGCA AGTATTTGGA 
GGGTATGGAG TAGCTGTGCC ACCAAAAGAC CCCTATACAA GAGAGAGGAT GCATGCCATG 
GAGAAACAGA TTGCCAGTTT AACTGGTCTT GTTCAGACTG CACTTTTGAA AGGGCCCAGC 
CCAAACAACT CTGCACCTAG CGACAGAGTC TTAAAATCAG CCAACGGCAG CGCTTCAAGC 
TCAGATCGGC CAGGAAATCA AGGTGTTGCT GCTGCAAAGA ATTCATTGGC CATTTCAGAA 
ACAATAACTG TTATCAACCA GCAGTCGTCT ACCTGCTCGC AGGAAATGCA CGTCAGCCTA 
CAGGACATGA GGCGCAATGT GGCCGAACTT CGTCTTCAGC TGCATAAGAT GAGACAACTG 
CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG ATGATGAAGA AAGCGGAGAT GGAAATCAGC 
AGCAAAGTGA CTGAGATGGT CAAGGGCCTG GAGGACCCGG TGCAGAGACA GCGCGTCTTG 
GTGGAACAAG AGCGCCAGAA GTACCTCGGT GACGAGGAAC AAATTGTTGC AAAGTTGTGT 
GACCTTGAAG ACTTTGTGGA ATCCCTTAAA AAGGATCAGA GCACATCCCA GAGGTCGATC 
ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC ATGCTGCGCC AGATAGGAGA GGCTGTTTCC 
AACCTGAAAG GAGAGTTTCC GTACCTCCAG AATAAGATGC GGGCCGTCTT GCGTGTGGAG 
GTGGAGGCCG TCAGATTTCT GAAAGAGGAG CCCCACAAAC TGGACAGCTT ACTGAAAAGG 
ATCCGAAACA TGACAGATGC CCTTTCTGCC TTAAGAAGAC ACGCCACTGA CGGATCCTCC 
AAATCCATTG ACAACAATCA GACCAGCCAG GTTCTAATAA CAGAAAGAGT AACTGAGACT 
GATTCACTCC AGGTCCATGA GGAAAAGTCC CAAGTGTACC TTCAGCCAAC ACAAACTGAT 
GGAGGAGCAG CAGTGGAATC GCCGACCACA TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG 
GCCGGCCTCA GAGTCCATCA AGTTCAAAGT TCTCCGGTTC TCATGCAGCA GTCCCAGCAC 
TCGTCTGCAC TCGTGAACCA CACTCCTGCC CCCAGTACCC AGAGCAGTCA CAGCACAGAA 
TTACTAGTGA GCCATCAAAC TGCCAGCGTG CCACCCAGCG TCACCCCAGA AAGTGCAAGG 
AGGAACCCAC CGACGGCCGC ACAGGAAGGA TCTTCCAAGC AAAGTCTTTT TATTGAGGAA 
ATGAGTCAAG TGACTTACAG GAACCGAGCG GTGTCCATTG AGGAGGCAGA GAGAAAATTT 
GAAGAGAAGA GACAGAATCT TGATCATTAT AACGGAAAGG AATTTGAGAA AATGCTGGAA 
GAAGCACAAG CCAGTATCAT GAAGGCCATT CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG 
AAAGTGGAGA TTGTGGACAA AAAAGAGGTC ACGGAGGTAA AAACCATAAT GGAGCAGGAT 
AACGATAAAG TAACAAAGTC TCCCCCTCCA CCACCACCTC GTCGTATGTA CCCACCAGGA 
TCTGGACTGA ACAGTGTATG GACAGCAGAA TCAACGTTCT TGGTCAGGAA GGACAGCAGC 
AAGGATTCGG AGGAAGCCCT TCCTAAGGTG CCATCAAAGG CAGAAACTGA AGAGCCTCAT 
CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT TCTGCCGTAA AGGATGAGGA TGAAGAAGAG 
GAAGGAGCAA GGATTATGGC TGAACTTCAG GCCTTCCAGA AGTGTTCTTT TGTGGATGTA 
AATCCTAAAA GCCCAAATGA GCTTTCTAAA GCAGATAGCC AAGATTACAG AGCTACAGCT 
GTAACCCAAG CTAAAGAGAA GAAGGTTTCA AAAGAGGGTT TAAATGCTGA AAAGCCAGAA 
GAAAAGTGGA AAATACAAAA TCCAGCATCT GAATCTGCAA AAATTAGGCA GGGAGATACT 
GTAATTGTGA ATGAGACCTT CAGTCTGTTT ACAGGAAGGG AGAAATATGG TGAGTGCCAA 
ACTGATAAAG AGATCTTACC TGCCCAGGAA TGTGAGAAAG GAGATGGGTC CCCACTTGTG 
AGCCCAGCAA TCCTTGAGAA GAGACTCATC AGTTTTAGCA CATCAAAAGA AACAGAGAGT 
GGGATCCCTC AACCCAAAAG AGAAGTGTTA AATGTCAAAG TTGGGGAAAT TTTGCATGTA 
GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC ATGACAGCTT TAAAGTCATT AAATGAGCAT 
GAAATGCCTT ATATGGACAT GGGCCAAACT GTTGTATTGA GACCTAAGTC ATCGAGAAGA 
AGTCTTAGTC AACAACAGTC TGATGACTCC AATTCTCCAA CTACGTCACC GACTGAAACA 
ACATCTCCAG CAGATAATAT TGCGTTCATG ATAACTAAAA CTAAAGTCCA GGTGCTTTCA 
ACTGGGGAGG TTCAGGACAT TGTCAGCCGT AAAGGAGAAG ATGTACAAAC CTTCAACATT 
GATAGAGGGA ATAACGAGAA AATACTCTCT GGGAATTTAG AAAACATTGG ATCTGAAGAC 
CCAGTGGTCT GCACTGACAA GAAACCAGTC ATCATCATAT TTGATGAACC AATGGATATT 
AAATCAGCAT ATAAGAGGCT TTCAACAATA TTTGAAGAAG GTGATGACGA TCTAGAAAAA 
CACATGTCAG AGGCTAAAAT AGACGAAGAA GATGAAGAAG AGGAAGCAGA AGTTCCTTCA 
CCCCAAGAAT CGAAAAAGGA GTTACCAATG GAACATACCA CAAAAGCATC CTCGGAGAAT 
CCAGGTTTGG AAAACAAGTA CAGATTCAAC TATCCAACTT TAGCCGAAAT GAAAAAGACC 
ATCTTCGAAG ACGCCAATGC CATACAGACC CATTTCACAG AGGAAGACAG GTCTGAAGCT 
TCCGATGATC AGCTTGGTCA AAGGCAGGAT GTCAAGAAAA AATTCAAGTT TAAATTTCCC 
AAAAAACAGC TCGCTGCGCT GACTCAAGCA ATCCGGACAG GGACAAAAAC TGGTAAGAAA 
ACATTACAGG TTGTGGTCTA CGAAGAGGAA GAAGAAGCAG ATGGGACTAT AAAACAACAC 
AAAGAAGCAA AGAGATTTGA AATTGCAAGA TCAAGTAGCA AAGAGGACAA TGCCAAATAC 
GGCGCAGAAG CAGTGGGACT TGAGACGGAC ATTCAGGCGT CACGGACAGA TGAGATTAAA 
AGAACCACCT ACAAGATTCT AGACAGTCTT GAGCAAACCA TAAAGCAGCA GGAAAACCTG 
ATGAGCGAAA TGGCACCCAT AATAGGGGCA GAGGCTTTAG AATCTAGAGA GAAATGTAAA 
GTCTCCAGTG ACATCAAGAC TGGTGCAGCG GAACCAGTGG AAGAACCAAA ACCTGTTGTA 
GAGACTCCTT CCTCCATCCC TACTGCAGCA CGTAAGGGTT CCAGTGGAGG AGCACAAACC 
AGCAGAATGC CAATCCCAAT GTCTTCTAAA AGTAGGCAAG CAAACGTGGA CAAAAGTAGC 
AAACAGCCCA AACTGCAGGA GCCGCAGCGC CAATACAGAC AGGCTAACGG AGGTACTAAG 
AAAGCTGGTG GGGATGTTAA GGCTTCTGCC CCTTCTTTAT CTGTTTCTAA GATTCCAGGC 
CTTTCCTCTA ACACTGGGAA AAGTGGCTCT GTGCCTGCTC AGTCTAGCGA GACCTCTAAC 
CCCCCTAATC CCTCTGTCAA GTCACATGTT GCCAATTCTA ACCCCCAAGC TAGCCGCACC 
GCTAACCCTT CAGCCCTTGT ACGTCCTGCT CACAATGGCT CATCCAAACT TCAGCCCCCG 
CCTACATATA AAGGTCACCA TCTTTCATTC TCACCGCAGG CCTCAAATGG CAGACCAGCT 
CTTCCCTCTA CTACTGCTTC TAGTTCTTCT CCCCCTTCTT CTGTCTCTCC CACTTCACTG 
ACTCAAGGTG TGAAGAGCAT CCGCACAATC CATACACCTA GCTTCACCAG CTACAAGTCA 
CAGAACGGAA GTGCAAGCAA AGGCCTGAAT GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915440.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915497.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.

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CloneID	OXa12044
Clone ID Related Accession (Same CDS sequence)	XM_018094778.1 , XM_004915440.3 , XM_004915440.4 , XM_018094778.2
Accession Version	XM_004915440.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915497.1
CDS	482..5737
Translation	MMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915440.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915440.4

ORF Insert Sequence:

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_018094778.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950267.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.

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CloneID	OXa12044
Clone ID Related Accession (Same CDS sequence)	XM_018094778.1 , XM_004915440.3 , XM_004915440.4 , XM_018094778.2
Accession Version	XM_018094778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950267.1
CDS	106..5361
Translation	MMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_018094778.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094778.2

ORF Insert Sequence:

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915443.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915500.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.

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CloneID	OXa12045
Clone ID Related Accession (Same CDS sequence)	XM_004915443.3 , XM_004915443.4
Accession Version	XM_004915443.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915443.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAG AACTTAGGGA CCATGGAAAA GGCACTGTGA ACATACAAGA CAGATGCTTC 
CTCAAAGTCT ACAATAAAGA CCCTGCGCAT GCATTTAACC ACACTCGTGG GGCGGTCAAT 
GGAGATGTAA GGATGCAACG AGATATTTCC TACTCGGTTC GGGAAGGCCC GGCGATCCAT 
AGGCCTGGCT CTGCTACATA CCCCTCCCAT GCTGGCCCAG TGTCACCCCC TCCCACACCT 
GTTCCCCACT CCATGCCATC ATCTCCATCA AGAATCCCCT ATGGCGGTGG GAGGCCTGCA 
GTGGTACAGA GCAACACCAT GCAGAGGGAA AGAATACCAA GCGTGCCGGC ACCTAGATCC 
ATATCTCCTA GCCCCAGTGC TATTTTGGAA AGGAGAGACG TAAAACCAGA TGAGGACATG 
GGCAATAAAA ATGTCCAGCT GCTAAGGAAT GACAGCATTT ACCAAGACCC TTATTTATAC 
CATGAAGGAC GGATGAGTAT GGCATCATCA CATGGTGGGC ACCCTCTCGA CGTCCCTGAT 
CATATTATTG CATACCACAG AGGGTCCTTG AGATCTGCAA GCACTTATTC TAATTCTTCT 
ATGCAAATGG AGATGATGGA ACAATCCATG TACAGACAAA AAAGCCGTAA GCACGTAGAG 
AGTCACTTAC CGACACTTGG TTCTAAAACT CCCCCAACCT CTCCTCACAG AGTGGCTGAT 
ATAAGGATGA TGGAGATGCA TGCCCATAAT GCCCATCTGC CTCCCCACGG CATGCAGGCC 
GACAGATCTT CTCCTCTCCG TCAGTCATTT AAAAAGGAGC AAGGGCCTGG CATGTTTGTA 
GAAGCTAAAG TGCGAAACAC AGGTGGAATA ATGGGCATGG CGGACATTGT CCCATCTCCT 
ACCGATAAGC AAGTATTTGG AGGGTATGGA GTAGCTGTGC CACCAAAAGA CCCCTATACA 
AGAGAGAGGA TGCATGCCAT GGAGAAACAG ATTGCCAGTT TAACTGGTCT TGTTCAGACT 
GCACTTTTGA AAGGGCCCAG CCCAAACAAC TCTGCACCTA GCGACAGAGT CTTAAAATCA 
GCCAACGGCA GCGCTTCAAG CTCAGATCGG CCAGGAAATC AAGGTGTTGC TGCTGCAAAG 
AATTCATTGG CCATTTCAGA AACAATAACT GTTATCAACC AGCAGTCGTC TACCTGCTCG 
CAGGAAATGC ACGTCAGCCT ACAGGACATG AGGCGCAATG TGGCCGAACT TCGTCTTCAG 
CTGCATAAGA TGAGACAACT GCAGCTGCAG AACCAGGAGA CGGTGAGGCT GATGATGAAG 
AAAGCGGAGA TGGAAATCAG CAGCAAAGTG ACTGAGATGG TCAAGGGCCT GGAGGACCCG 
GTGCAGAGAC AGCGCGTCTT GGTGGAACAA GAGCGCCAGA AGTACCTCGG TGACGAGGAA 
CAAATTGTTG CAAAGTTGTG TGACCTTGAA GACTTTGTGG AATCCCTTAA AAAGGATCAG 
AGCACATCCC AGAGGTCGAT CACCCTAAAG GACGTTGAGG ATCGGGCCTT CATGCTGCGC 
CAGATAGGAG AGGCTGTTTC CAACCTGAAA GGAGAGTTTC CGTACCTCCA GAATAAGATG 
CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC GTCAGATTTC TGAAAGAGGA GCCCCACAAA 
CTGGACAGCT TACTGAAAAG GATCCGAAAC ATGACAGATG CCCTTTCTGC CTTAAGAAGA 
CACGCCACTG ACGGATCCTC CAAATCCATT GACAACAATC AGACCAGCCA GGTTCTAATA 
ACAGAAAGAG TAACTGAGAC TGATTCACTC CAGGTCCATG AGGAAAAGTC CCAAGTGTAC 
CTTCAGCCAA CACAAACTGA TGGAGGAGCA GCAGTGGAAT CGCCGACCAC ATCTGTTAAA 
TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC AGAGTCCATC AAGTTCAAAG TTCTCCGGTT 
CTCATGCAGC AGTCCCAGCA CTCGTCTGCA CTCGTGAACC ACACTCCTGC CCCCAGTACC 
CAGAGCAGTC ACAGCACAGA ATTACTAGTG AGCCATCAAA CTGCCAGCGT GCCACCCAGC 
GTCACCCCAG AAAGTGCAAG GAGGAACCCA CCGACGGCCG CACAGGAAGG ATCTTCCAAG 
CAAAGTCTTT TTATTGAGGA AATGAGTCAA GTGACTTACA GGAACCGAGC GGTGTCCATT 
GAGGAGGCAG AGAGAAAATT TGAAGAGAAG AGACAGAATC TTGATCATTA TAACGGAAAG 
GAATTTGAGA AAATGCTGGA AGAAGCACAA GCCAGTATCA TGAAGGCCAT TCCCAGCTTA 
GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG ATTGTGGACA AAAAAGAGGT CACGGAGGTA 
AAAACCATAA TGGAGCAGGA TAACGATAAA GTAACAAAGT CTCCCCCTCC ACCACCACCT 
CGTCGTATGT ACCCACCAGG ATCTGGACTG AACAGTGTAT GGACAGCAGA ATCAACGTTC 
TTGGTCAGGA AGGACAGCAG CAAGGATTCG GAGGAAGCCC TTCCTAAGGT GCCATCAAAG 
GCAGAAACTG AAGAGCCTCA TCTTCAGTCG GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA 
AAGGATGAGG ATGAAGAAGA GGAAGGAGCA AGGATTATGG CTGAACTTCA GGCCTTCCAG 
AAGTGTTCTT TTGTGGATGT AAATCCTAAA AGCCCAAATG AGCTTTCTAA AGCAGATAGC 
CAAGATTACA GAGCTACAGC TGTAACCCAA GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT 
TTAAATGCTG AAAAGCCAGA AGAAAAGTGG AAAATACAAA ATCCAGCATC TGAATCTGCA 
AAAATTAGGC AGGGAGATAC TGTAATTGTG AATGAGACCT TCAGTCTGTT TACAGGAAGG 
GAGAAATATG GTGAGTGCCA AACTGATAAA GAGATCTTAC CTGCCCAGGA ATGTGAGAAA 
GGAGATGGGT CCCCACTTGT GAGCCCAGCA ATCCTTGAGA AGAGACTCAT CAGTTTTAGC 
ACATCAAAAG AAACAGAGAG TGGGATCCCT CAACCCAAAA GAGAAGTGTT AAATGTCAAA 
GTTGGGGAAA TTTTGCATGT AGAAAAGAAG GAAGAGGTGC TAGAAAGTCA CATGACAGCT 
TTAAAGTCAT TAAATGAGCA TGAAATGCCT TATATGGACA TGGGCCAAAC TGTTGTATTG 
AGACCTAAGT CATCGAGAAG AAGTCTTAGT CAACAACAGT CTGATGACTC CAATTCTCCA 
ACTACGTCAC CGACTGAAAC AACATCTCCA GCAGATAATA TTGCGTTCAT GATAACTAAA 
ACTAAAGTCC AGGTGCTTTC AACTGGGGAG GTTCAGGACA TTGTCAGCCG TAAAGGAGAA 
GATGTACAAA CCTTCAACAT TGATAGAGGG AATAACGAGA AAATACTCTC TGGGAATTTA 
GAAAACATTG GATCTGAAGA CCCAGTGGTC TGCACTGACA AGAAACCAGT CATCATCATA 
TTTGATGAAC CAATGGATAT TAAATCAGCA TATAAGAGGC TTTCAACAAT ATTTGAAGAA 
GGTGATGACG ATCTAGAAAA ACACATGTCA GAGGCTAAAA TAGACGAAGA AGATGAAGAA 
GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA TCGAAAAAGG AGTTACCAAT GGAACATACC 
ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG GAAAACAAGT ACAGATTCAA CTATCCAACT 
TTAGCCGAAA TGAAAAAGAC CATCTTCGAA GACGCCAATG CCATACAGAC CCATTTCACA 
GAGGAAGACA GGTCTGAAGC TTCCGATGAT CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA 
AAATTCAAGT TTAAATTTCC CAAAAAACAG CTCGCTGCGC TGACTCAAGC AATCCGGACA 
GGGACAAAAA CTGGTAAGAA AACATTACAG GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA 
GATGGGACTA TAAAACAACA CAAAGAAGCA AAGAGATTTG AAATTGCAAG ATCAAGTAGC 
AAAGAGGACA ATGCCAAATA CGGCGCAGAA GCAGTGGGAC TTGAGACGGA CATTCAGGCG 
TCACGGACAG ATGAGATTAA AAGAACCACC TACAAGATTC TAGACAGTCT TGAGCAAACC 
ATAAAGCAGC AGGAAAACCT GATGAGCGAA ATGGCACCCA TAATAGGGGC AGAGGCTTTA 
GAATCTAGAG AGAAATGTAA AGTCTCCAGT GACATCAAGA CTGGTGCAGC GGAACCAGTG 
GAAGAACCAA AACCTGTTGT AGAGACTCCT TCCTCCATCC CTACTGCAGC ACGTAAGGGT 
TCCAGTGGAG GAGCACAAAC CAGCAGAATG CCAATCCCAA TGTCTTCTAA AAGTAGGCAA 
GCAAACGTGG ACAAAAGTAG CAAACAGCCC AAACTGCAGG AGCCGCAGCG CCAATACAGA 
CAGGCTAACG GAGGTACTAA GAAAGCTGGT GGGGATGTTA AGGCTTCTGC CCCTTCTTTA 
TCTGTTTCTA AGATTCCAGG CCTTTCCTCT AACACTGGGA AAAGTGGCTC TGTGCCTGCT 
CAGTCTAGCG AGACCTCTAA CCCCCCTAAT CCCTCTGTCA AGTCACATGT TGCCAATTCT 
AACCCCCAAG CTAGCCGCAC CGCTAACCCT TCAGCCCTTG TACGTCCTGC TCACAATGGC 
TCATCCAAAC TTCAGCCCCC GCCTACATAT AAAGGTCACC ATCTTTCATT CTCACCGCAG 
GCCTCAAATG GCAGACCAGC TCTTCCCTCT ACTACTGCTT CTAGTTCTTC TCCCCCTTCT 
TCTGTCTCTC CCACTTCACT GACTCAAGGT GTGAAGAGCA TCCGCACAAT CCATACACCT 
AGCTTCACCA GCTACAAGTC ACAGAACGGA AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG 
ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915500.1
CDS	236..5224
Translation	MQTELRDHGKGTVNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915443.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915443.4

ORF Insert Sequence:

ATGCAGACAG AACTTAGGGA CCATGGAAAA GGCACTGTGA ACATACAAGA CAGATGCTTC 
CTCAAAGTCT ACAATAAAGA CCCTGCGCAT GCATTTAACC ACACTCGTGG GGCGGTCAAT 
GGAGATGTAA GGATGCAACG AGATATTTCC TACTCGGTTC GGGAAGGCCC GGCGATCCAT 
AGGCCTGGCT CTGCTACATA CCCCTCCCAT GCTGGCCCAG TGTCACCCCC TCCCACACCT 
GTTCCCCACT CCATGCCATC ATCTCCATCA AGAATCCCCT ATGGCGGTGG GAGGCCTGCA 
GTGGTACAGA GCAACACCAT GCAGAGGGAA AGAATACCAA GCGTGCCGGC ACCTAGATCC 
ATATCTCCTA GCCCCAGTGC TATTTTGGAA AGGAGAGACG TAAAACCAGA TGAGGACATG 
GGCAATAAAA ATGTCCAGCT GCTAAGGAAT GACAGCATTT ACCAAGACCC TTATTTATAC 
CATGAAGGAC GGATGAGTAT GGCATCATCA CATGGTGGGC ACCCTCTCGA CGTCCCTGAT 
CATATTATTG CATACCACAG AGGGTCCTTG AGATCTGCAA GCACTTATTC TAATTCTTCT 
ATGCAAATGG AGATGATGGA ACAATCCATG TACAGACAAA AAAGCCGTAA GCACGTAGAG 
AGTCACTTAC CGACACTTGG TTCTAAAACT CCCCCAACCT CTCCTCACAG AGTGGCTGAT 
ATAAGGATGA TGGAGATGCA TGCCCATAAT GCCCATCTGC CTCCCCACGG CATGCAGGCC 
GACAGATCTT CTCCTCTCCG TCAGTCATTT AAAAAGGAGC AAGGGCCTGG CATGTTTGTA 
GAAGCTAAAG TGCGAAACAC AGGTGGAATA ATGGGCATGG CGGACATTGT CCCATCTCCT 
ACCGATAAGC AAGTATTTGG AGGGTATGGA GTAGCTGTGC CACCAAAAGA CCCCTATACA 
AGAGAGAGGA TGCATGCCAT GGAGAAACAG ATTGCCAGTT TAACTGGTCT TGTTCAGACT 
GCACTTTTGA AAGGGCCCAG CCCAAACAAC TCTGCACCTA GCGACAGAGT CTTAAAATCA 
GCCAACGGCA GCGCTTCAAG CTCAGATCGG CCAGGAAATC AAGGTGTTGC TGCTGCAAAG 
AATTCATTGG CCATTTCAGA AACAATAACT GTTATCAACC AGCAGTCGTC TACCTGCTCG 
CAGGAAATGC ACGTCAGCCT ACAGGACATG AGGCGCAATG TGGCCGAACT TCGTCTTCAG 
CTGCATAAGA TGAGACAACT GCAGCTGCAG AACCAGGAGA CGGTGAGGCT GATGATGAAG 
AAAGCGGAGA TGGAAATCAG CAGCAAAGTG ACTGAGATGG TCAAGGGCCT GGAGGACCCG 
GTGCAGAGAC AGCGCGTCTT GGTGGAACAA GAGCGCCAGA AGTACCTCGG TGACGAGGAA 
CAAATTGTTG CAAAGTTGTG TGACCTTGAA GACTTTGTGG AATCCCTTAA AAAGGATCAG 
AGCACATCCC AGAGGTCGAT CACCCTAAAG GACGTTGAGG ATCGGGCCTT CATGCTGCGC 
CAGATAGGAG AGGCTGTTTC CAACCTGAAA GGAGAGTTTC CGTACCTCCA GAATAAGATG 
CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC GTCAGATTTC TGAAAGAGGA GCCCCACAAA 
CTGGACAGCT TACTGAAAAG GATCCGAAAC ATGACAGATG CCCTTTCTGC CTTAAGAAGA 
CACGCCACTG ACGGATCCTC CAAATCCATT GACAACAATC AGACCAGCCA GGTTCTAATA 
ACAGAAAGAG TAACTGAGAC TGATTCACTC CAGGTCCATG AGGAAAAGTC CCAAGTGTAC 
CTTCAGCCAA CACAAACTGA TGGAGGAGCA GCAGTGGAAT CGCCGACCAC ATCTGTTAAA 
TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC AGAGTCCATC AAGTTCAAAG TTCTCCGGTT 
CTCATGCAGC AGTCCCAGCA CTCGTCTGCA CTCGTGAACC ACACTCCTGC CCCCAGTACC 
CAGAGCAGTC ACAGCACAGA ATTACTAGTG AGCCATCAAA CTGCCAGCGT GCCACCCAGC 
GTCACCCCAG AAAGTGCAAG GAGGAACCCA CCGACGGCCG CACAGGAAGG ATCTTCCAAG 
CAAAGTCTTT TTATTGAGGA AATGAGTCAA GTGACTTACA GGAACCGAGC GGTGTCCATT 
GAGGAGGCAG AGAGAAAATT TGAAGAGAAG AGACAGAATC TTGATCATTA TAACGGAAAG 
GAATTTGAGA AAATGCTGGA AGAAGCACAA GCCAGTATCA TGAAGGCCAT TCCCAGCTTA 
GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG ATTGTGGACA AAAAAGAGGT CACGGAGGTA 
AAAACCATAA TGGAGCAGGA TAACGATAAA GTAACAAAGT CTCCCCCTCC ACCACCACCT 
CGTCGTATGT ACCCACCAGG ATCTGGACTG AACAGTGTAT GGACAGCAGA ATCAACGTTC 
TTGGTCAGGA AGGACAGCAG CAAGGATTCG GAGGAAGCCC TTCCTAAGGT GCCATCAAAG 
GCAGAAACTG AAGAGCCTCA TCTTCAGTCG GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA 
AAGGATGAGG ATGAAGAAGA GGAAGGAGCA AGGATTATGG CTGAACTTCA GGCCTTCCAG 
AAGTGTTCTT TTGTGGATGT AAATCCTAAA AGCCCAAATG AGCTTTCTAA AGCAGATAGC 
CAAGATTACA GAGCTACAGC TGTAACCCAA GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT 
TTAAATGCTG AAAAGCCAGA AGAAAAGTGG AAAATACAAA ATCCAGCATC TGAATCTGCA 
AAAATTAGGC AGGGAGATAC TGTAATTGTG AATGAGACCT TCAGTCTGTT TACAGGAAGG 
GAGAAATATG GTGAGTGCCA AACTGATAAA GAGATCTTAC CTGCCCAGGA ATGTGAGAAA 
GGAGATGGGT CCCCACTTGT GAGCCCAGCA ATCCTTGAGA AGAGACTCAT CAGTTTTAGC 
ACATCAAAAG AAACAGAGAG TGGGATCCCT CAACCCAAAA GAGAAGTGTT AAATGTCAAA 
GTTGGGGAAA TTTTGCATGT AGAAAAGAAG GAAGAGGTGC TAGAAAGTCA CATGACAGCT 
TTAAAGTCAT TAAATGAGCA TGAAATGCCT TATATGGACA TGGGCCAAAC TGTTGTATTG 
AGACCTAAGT CATCGAGAAG AAGTCTTAGT CAACAACAGT CTGATGACTC CAATTCTCCA 
ACTACGTCAC CGACTGAAAC AACATCTCCA GCAGATAATA TTGCGTTCAT GATAACTAAA 
ACTAAAGTCC AGGTGCTTTC AACTGGGGAG GTTCAGGACA TTGTCAGCCG TAAAGGAGAA 
GATGTACAAA CCTTCAACAT TGATAGAGGG AATAACGAGA AAATACTCTC TGGGAATTTA 
GAAAACATTG GATCTGAAGA CCCAGTGGTC TGCACTGACA AGAAACCAGT CATCATCATA 
TTTGATGAAC CAATGGATAT TAAATCAGCA TATAAGAGGC TTTCAACAAT ATTTGAAGAA 
GGTGATGACG ATCTAGAAAA ACACATGTCA GAGGCTAAAA TAGACGAAGA AGATGAAGAA 
GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA TCGAAAAAGG AGTTACCAAT GGAACATACC 
ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG GAAAACAAGT ACAGATTCAA CTATCCAACT 
TTAGCCGAAA TGAAAAAGAC CATCTTCGAA GACGCCAATG CCATACAGAC CCATTTCACA 
GAGGAAGACA GGTCTGAAGC TTCCGATGAT CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA 
AAATTCAAGT TTAAATTTCC CAAAAAACAG CTCGCTGCGC TGACTCAAGC AATCCGGACA 
GGGACAAAAA CTGGTAAGAA AACATTACAG GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA 
GATGGGACTA TAAAACAACA CAAAGAAGCA AAGAGATTTG AAATTGCAAG ATCAAGTAGC 
AAAGAGGACA ATGCCAAATA CGGCGCAGAA GCAGTGGGAC TTGAGACGGA CATTCAGGCG 
TCACGGACAG ATGAGATTAA AAGAACCACC TACAAGATTC TAGACAGTCT TGAGCAAACC 
ATAAAGCAGC AGGAAAACCT GATGAGCGAA ATGGCACCCA TAATAGGGGC AGAGGCTTTA 
GAATCTAGAG AGAAATGTAA AGTCTCCAGT GACATCAAGA CTGGTGCAGC GGAACCAGTG 
GAAGAACCAA AACCTGTTGT AGAGACTCCT TCCTCCATCC CTACTGCAGC ACGTAAGGGT 
TCCAGTGGAG GAGCACAAAC CAGCAGAATG CCAATCCCAA TGTCTTCTAA AAGTAGGCAA 
GCAAACGTGG ACAAAAGTAG CAAACAGCCC AAACTGCAGG AGCCGCAGCG CCAATACAGA 
CAGGCTAACG GAGGTACTAA GAAAGCTGGT GGGGATGTTA AGGCTTCTGC CCCTTCTTTA 
TCTGTTTCTA AGATTCCAGG CCTTTCCTCT AACACTGGGA AAAGTGGCTC TGTGCCTGCT 
CAGTCTAGCG AGACCTCTAA CCCCCCTAAT CCCTCTGTCA AGTCACATGT TGCCAATTCT 
AACCCCCAAG CTAGCCGCAC CGCTAACCCT TCAGCCCTTG TACGTCCTGC TCACAATGGC 
TCATCCAAAC TTCAGCCCCC GCCTACATAT AAAGGTCACC ATCTTTCATT CTCACCGCAG 
GCCTCAAATG GCAGACCAGC TCTTCCCTCT ACTACTGCTT CTAGTTCTTC TCCCCCTTCT 
TCTGTCTCTC CCACTTCACT GACTCAAGGT GTGAAGAGCA TCCGCACAAT CCATACACCT 
AGCTTCACCA GCTACAAGTC ACAGAACGGA AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG 
ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965644.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821098.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.

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CloneID	OXa36492
Clone ID Related Accession (Same CDS sequence)	XM_012965644.2 , XM_012965644.3
Accession Version	XM_012965644.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821098.1
CDS	317..6136
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965644.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965644.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965645.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821099.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.

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CloneID	OXa36493
Clone ID Related Accession (Same CDS sequence)	XM_012965645.2 , XM_012965645.3
Accession Version	XM_012965645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGGTTCCAG TGGAGGAGCA CAAACCAGCA GAATGCCAAT 
CCCAATGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821099.1
CDS	318..5672
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARFQWRSTNQQNANPNVF

Target ORF information:

RefSeq Version	XM_012965645.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965645.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGGTTCCAG TGGAGGAGCA CAAACCAGCA GAATGCCAAT 
CCCAATGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965646.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821100.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.

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CloneID	OXa36494
Clone ID Related Accession (Same CDS sequence)	XM_012965646.3 , XM_012965646.2
Accession Version	XM_012965646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGGCCGTG 
CAGTGCATGT TGGCCACACT TCATTCTGAA CTTGACATTC AAAGGTACTT GATGAAAATT 
GAATCCTCTC AGAGAACAAA AAGTCCGAAG CTGTCTTCAA GCCCACAGCC GAGCGTAGGA 
GACCCGGCAG AACATCTGTC AGAGGCGTCT GCTGATTCCG GAGACATGAT GTTCGAAGCG 
GATTCGGCAG TGCCGTTTGT CCGAGGCAGT CGCACAAGAG CCAGCCTTCC GGTGGTCAGA 
TCCACAAATC AAACGAAAGA GAGATCACTT GGTACGCTCT TCCTACAGTA TGGCGACGAT 
ACCAAGAAAA TGCAGATGCC CAATGAAATC ACAAGCGTAG ACACGATCCG TGCCCTGTTC 
GTAAGTGCCT TCCCCCAGCA ACTGACAATG AAGATCCTGG AATCGCCCAG CACAGCCATT 
TATATTAAGG ATGAGGGCCG AAATGTCTAT TATGAGCTCA GCGATGTCAG GAACATACAA 
GACAGATGCT TCCTCAAAGT CTACAATAAA GACCCTGCGC ATGCATTTAA CCACACTCGT 
GGGGCGGTCA ATGGAGATGT AAGGATGCAA CGAGATATTT CCTACTCGGT TCGGGAAGGC 
CCGGCGATCC ATAGGCCTGG CTCTGCTACA TACCCCTCCC ATGCTGGCCC AGTGTCACCC 
CCTCCCACAC CTGTTCCCCA CTCCATGCCA TCATCTCCAT CAAGAATCCC CTATGGCGGT 
GGGAGGCCTG CAGTGGTACA GAGCAACACC ATGCAGAGGG AAAGAATACC AAGCGTGCCG 
GCACCTAGAT CCATATCTCC TAGCCCCAGT GCTATTTTGG AAAGGAGAGA CGTAAAACCA 
GATGAGGACA TGGGCAATAA AAATGTCCAG CTGCTAAGGA ATGACAGCAT TTACCAAGAC 
CCTTATTTAT ACCATGAAGG ACGGATGAGT ATGGCATCAT CACATGGTGG GCACCCTCTC 
GACGTCCCTG ATCATATTAT TGCATACCAC AGAGGGTCCT TGAGATCTGC AAGCACTTAT 
TCTAATTCTT CTATGCAAAT GGAGATGATG GAACAATCCA TGTACAGACA AAAAAGCCGT 
AAGCACGTAG AGAGTCACTT ACCGACACTT GGTTCTAAAA CTCCCCCAAC CTCTCCTCAC 
AGAGTGGCTG ATATAAGGAT GATGGAGATG CATGCCCATA ATGCCCATCT GCCTCCCCAC 
GGCATGCAGG CCGACAGATC TTCTCCTCTC CGTCAGTCAT TTAAAAAGGA GCAAGGGCCT 
GGCATGTTTG TAGAAGCTAA AGTGCGAAAC ACAGGTGGAA TAATGGGCAT GGCGGACATT 
GTCCCATCTC CTACCGATAA GCAAGTATTT GGAGGGTATG GAGTAGCTGT GCCACCAAAA 
GACCCCTATA CAAGAGAGAG GATGCATGCC ATGGAGAAAC AGATTGCCAG TTTAACTGGT 
CTTGTTCAGA CTGCACTTTT GAAAGGGCCC AGCCCAAACA ACTCTGCACC TAGCGACAGA 
GTCTTAAAAT CAGCCAACGG CAGCGCTTCA AGCTCAGATC GGCCAGGAAA TCAAGGTGTT 
GCTGCTGCAA AGAATTCATT GGCCATTTCA GAAACAATAA CTGTTATCAA CCAGCAGTCG 
TCTACCTGCT CGCAGGAAAT GCACGTCAGC CTACAGGACA TGAGGCGCAA TGTGGCCGAA 
CTTCGTCTTC AGCTGCATAA GATGAGACAA CTGCAGCTGC AGAACCAGGA GACGGTGAGG 
CTGATGATGA AGAAAGCGGA GATGGAAATC AGCAGCAAAG TGACTGAGAT GGTCAAGGGC 
CTGGAGGACC CGGTGCAGAG ACAGCGCGTC TTGGTGGAAC AAGAGCGCCA GAAGTACCTC 
GGTGACGAGG AACAAATTGT TGCAAAGTTG TGTGACCTTG AAGACTTTGT GGAATCCCTT 
AAAAAGGATC AGAGCACATC CCAGAGGTCG ATCACCCTAA AGGACGTTGA GGATCGGGCC 
TTCATGCTGC GCCAGATAGG AGAGGCTGTT TCCAACCTGA AAGGAGAGTT TCCGTACCTC 
CAGAATAAGA TGCGGGCCGT CTTGCGTGTG GAGGTGGAGG CCGTCAGATT TCTGAAAGAG 
GAGCCCCACA AACTGGACAG CTTACTGAAA AGGATCCGAA ACATGACAGA TGCCCTTTCT 
GCCTTAAGAA GACACGCCAC TGACGGATCC TCCAAATCCA TTGACAACAA TCAGACCAGC 
CAGGTTCTAA TAACAGAAAG AGTAACTGAG ACTGATTCAC TCCAGGTCCA TGAGGAAAAG 
TCCCAAGTGT ACCTTCAGCC AACACAAACT GATGGAGGAG CAGCAGTGGA ATCGCCGACC 
ACATCTGTTA AATCGGAAGT GGTTCCCCTG TCGGCCGGCC TCAGAGTCCA TCAAGTTCAA 
AGTTCTCCGG TTCTCATGCA GCAGTCCCAG CACTCGTCTG CACTCGTGAA CCACACTCCT 
GCCCCCAGTA CCCAGAGCAG TCACAGCACA GAATTACTAG TGAGCCATCA AACTGCCAGC 
GTGCCACCCA GCGTCACCCC AGAAAGTGCA AGGAGGAACC CACCGACGGC CGCACAGGAA 
GGATCTTCCA AGCAAAGTCT TTTTATTGAG GAAATGAGTC AAGTGACTTA CAGGAACCGA 
GCGGTGTCCA TTGAGGAGGC AGAGAGAAAA TTTGAAGAGA AGAGACAGAA TCTTGATCAT 
TATAACGGAA AGGAATTTGA GAAAATGCTG GAAGAAGCAC AAGCCAGTAT CATGAAGGCC 
ATTCCCAGCT TAGAGGTGCC AGCCCAGTCC CAGAAAGTGG AGATTGTGGA CAAAAAAGAG 
GTCACGGAGG TAAAAACCAT AATGGAGCAG GATAACGATA AAGTAACAAA GTCTCCCCCT 
CCACCACCAC CTCGTCGTAT GTACCCACCA GGATCTGGAC TGAACAGTGT ATGGACAGCA 
GAATCAACGT TCTTGGTCAG GAAGGACAGC AGCAAGGATT CGGAGGAAGC CCTTCCTAAG 
GTGCCATCAA AGGCAGAAAC TGAAGAGCCT CATCTTCAGT CGGTGGAGGC TACAGTCAAG 
TCTTCTGCCG TAAAGGATGA GGATGAAGAA GAGGAAGGAG CAAGGATTAT GGCTGAACTT 
CAGGCCTTCC AGAAGTGTTC TTTTGTGGAT GTAAATCCTA AAAGCCCAAA TGAGCTTTCT 
AAAGCAGATA GCCAAGATTA CAGAGCTACA GCTGTAACCC AAGCTAAAGA GAAGAAGGTT 
TCAAAAGAGG GTTTAAATGC TGAAAAGCCA GAAGAAAAGT GGAAAATACA AAATCCAGCA 
TCTGAATCTG CAAAAATTAG GCAGGGAGAT ACTGTAATTG TGAATGAGAC CTTCAGTCTG 
TTTACAGGAA GGGAGAAATA TGGTGAGTGC CAAACTGATA AAGAGATCTT ACCTGCCCAG 
GAATGTGAGA AAGGAGATGG GTCCCCACTT GTGAGCCCAG CAATCCTTGA GAAGAGACTC 
ATCAGTTTTA GCACATCAAA AGAAACAGAG AGTGGGATCC CTCAACCCAA AAGAGAAGTG 
TTAAATGTCA AAGTTGGGGA AATTTTGCAT GTAGAAAAGA AGGAAGAGGT GCTAGAAAGT 
CACATGACAG CTTTAAAGTC ATTAAATGAG CATGAAATGC CTTATATGGA CATGGGCCAA 
ACTGTTGTAT TGAGACCTAA GTCATCGAGA AGAAGTCTTA GTCAACAACA GTCTGATGAC 
TCCAATTCTC CAACTACGTC ACCGACTGAA ACAACATCTC CAGCAGATAA TATTGCGTTC 
ATGATAACTA AAACTAAAGT CCAGGTGCTT TCAACTGGGG AGGTTCAGGA CATTGTCAGC 
CGTAAAGGAG AAGATGTACA AACCTTCAAC ATTGATAGAG GGAATAACGA GAAAATACTC 
TCTGGGAATT TAGAAAACAT TGGATCTGAA GACCCAGTGG TCTGCACTGA CAAGAAACCA 
GTCATCATCA TATTTGATGA ACCAATGGAT ATTAAATCAG CATATAAGAG GCTTTCAACA 
ATATTTGAAG AAGGTGATGA CGATCTAGAA AAACACATGT CAGAGGCTAA AATAGACGAA 
GAAGATGAAG AAGAGGAAGC AGAAGTTCCT TCACCCCAAG AATCGAAAAA GGAGTTACCA 
ATGGAACATA CCACAAAAGC ATCCTCGGAG AATCCAGGTT TGGAAAACAA GTACAGATTC 
AACTATCCAA CTTTAGCCGA AATGAAAAAG ACCATCTTCG AAGACGCCAA TGCCATACAG 
ACCCATTTCA CAGAGGAAGA CAGGTCTGAA GCTTCCGATG ATCAGCTTGG TCAAAGGCAG 
GATGTCAAGA AAAAATTCAA GTTTAAATTT CCCAAAAAAC AGCTCGCTGC GCTGACTCAA 
GCAATCCGGA CAGGGACAAA AACTGGTAAG AAAACATTAC AGGTTGTGGT CTACGAAGAG 
GAAGAAGAAG CAGATGGGAC TATAAAACAA CACAAAGAAG CAAAGAGATT TGAAATTGCA 
AGATCAAGTA GCAAAGAGGA CAATGCCAAA TACGGCGCAG AAGCAGTGGG ACTTGAGACG 
GACATTCAGG CGTCACGGAC AGATGAGATT AAAAGAACCA CCTACAAGAT TCTAGACAGT 
CTTGAGCAAA CCATAAAGCA GCAGGAAAAC CTGATGAGCG AAATGGCACC CATAATAGGG 
GCAGAGGCTT TAGAATCTAG AGAGAAATGT AAAGTCTCCA GTGACATCAA GACTGGTGCA 
GCGGAACCAG TGGAAGAACC AAAACCTGTT GTAGAGACTC CTTCCTCCAT CCCTACTGCA 
GCACGTAAGG GTTCCAGTGG AGGAGCACAA ACCAGCAGAA TGCCAATCCC AATGTCTTCT 
AAAAGTAGGC AAGCAAACGT GGACAAAAGT AGCAAACAGC CCAAACTGCA GGAGCCGCAG 
CGCCAATACA GACAGGCTAA CGGAGGTACT AAGAAAGCTG GTGGGGATGT TAAGGCTTCT 
GCCCCTTCTT TATCTGTTTC TAAGATTCCA GGCCTTTCCT CTAACACTGG GAAAAGTGGC 
TCTGTGCCTG CTCAGTCTAG CGAGACCTCT AACCCCCCTA ATCCCTCTGT CAAGTCACAT 
GTTGCCAATT CTAACCCCCA AGCTAGCCGC ACCGCTAACC CTTCAGCCCT TGTACGTCCT 
GCTCACAATG GCTCATCCAA ACTTCAGCCC CCGCCTACAT ATAAAGGTCA CCATCTTTCA 
TTCTCACCGC AGGCCTCAAA TGGCAGACCA GCTCTTCCCT CTACTACTGC TTCTAGTTCT 
TCTCCCCCTT CTTCTGTCTC TCCCACTTCA CTGACTCAAG GTGTGAAGAG CATCCGCACA 
ATCCATACAC CTAGCTTCAC CAGCTACAAG TCACAGAACG GAAGTGCAAG CAAAGGCCTG 
AATGCCAAGG AGACCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821100.1
CDS	254..5794
Translation	MQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965646.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965646.3

ORF Insert Sequence:

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGGCCGTG 
CAGTGCATGT TGGCCACACT TCATTCTGAA CTTGACATTC AAAGGTACTT GATGAAAATT 
GAATCCTCTC AGAGAACAAA AAGTCCGAAG CTGTCTTCAA GCCCACAGCC GAGCGTAGGA 
GACCCGGCAG AACATCTGTC AGAGGCGTCT GCTGATTCCG GAGACATGAT GTTCGAAGCG 
GATTCGGCAG TGCCGTTTGT CCGAGGCAGT CGCACAAGAG CCAGCCTTCC GGTGGTCAGA 
TCCACAAATC AAACGAAAGA GAGATCACTT GGTACGCTCT TCCTACAGTA TGGCGACGAT 
ACCAAGAAAA TGCAGATGCC CAATGAAATC ACAAGCGTAG ACACGATCCG TGCCCTGTTC 
GTAAGTGCCT TCCCCCAGCA ACTGACAATG AAGATCCTGG AATCGCCCAG CACAGCCATT 
TATATTAAGG ATGAGGGCCG AAATGTCTAT TATGAGCTCA GCGATGTCAG GAACATACAA 
GACAGATGCT TCCTCAAAGT CTACAATAAA GACCCTGCGC ATGCATTTAA CCACACTCGT 
GGGGCGGTCA ATGGAGATGT AAGGATGCAA CGAGATATTT CCTACTCGGT TCGGGAAGGC 
CCGGCGATCC ATAGGCCTGG CTCTGCTACA TACCCCTCCC ATGCTGGCCC AGTGTCACCC 
CCTCCCACAC CTGTTCCCCA CTCCATGCCA TCATCTCCAT CAAGAATCCC CTATGGCGGT 
GGGAGGCCTG CAGTGGTACA GAGCAACACC ATGCAGAGGG AAAGAATACC AAGCGTGCCG 
GCACCTAGAT CCATATCTCC TAGCCCCAGT GCTATTTTGG AAAGGAGAGA CGTAAAACCA 
GATGAGGACA TGGGCAATAA AAATGTCCAG CTGCTAAGGA ATGACAGCAT TTACCAAGAC 
CCTTATTTAT ACCATGAAGG ACGGATGAGT ATGGCATCAT CACATGGTGG GCACCCTCTC 
GACGTCCCTG ATCATATTAT TGCATACCAC AGAGGGTCCT TGAGATCTGC AAGCACTTAT 
TCTAATTCTT CTATGCAAAT GGAGATGATG GAACAATCCA TGTACAGACA AAAAAGCCGT 
AAGCACGTAG AGAGTCACTT ACCGACACTT GGTTCTAAAA CTCCCCCAAC CTCTCCTCAC 
AGAGTGGCTG ATATAAGGAT GATGGAGATG CATGCCCATA ATGCCCATCT GCCTCCCCAC 
GGCATGCAGG CCGACAGATC TTCTCCTCTC CGTCAGTCAT TTAAAAAGGA GCAAGGGCCT 
GGCATGTTTG TAGAAGCTAA AGTGCGAAAC ACAGGTGGAA TAATGGGCAT GGCGGACATT 
GTCCCATCTC CTACCGATAA GCAAGTATTT GGAGGGTATG GAGTAGCTGT GCCACCAAAA 
GACCCCTATA CAAGAGAGAG GATGCATGCC ATGGAGAAAC AGATTGCCAG TTTAACTGGT 
CTTGTTCAGA CTGCACTTTT GAAAGGGCCC AGCCCAAACA ACTCTGCACC TAGCGACAGA 
GTCTTAAAAT CAGCCAACGG CAGCGCTTCA AGCTCAGATC GGCCAGGAAA TCAAGGTGTT 
GCTGCTGCAA AGAATTCATT GGCCATTTCA GAAACAATAA CTGTTATCAA CCAGCAGTCG 
TCTACCTGCT CGCAGGAAAT GCACGTCAGC CTACAGGACA TGAGGCGCAA TGTGGCCGAA 
CTTCGTCTTC AGCTGCATAA GATGAGACAA CTGCAGCTGC AGAACCAGGA GACGGTGAGG 
CTGATGATGA AGAAAGCGGA GATGGAAATC AGCAGCAAAG TGACTGAGAT GGTCAAGGGC 
CTGGAGGACC CGGTGCAGAG ACAGCGCGTC TTGGTGGAAC AAGAGCGCCA GAAGTACCTC 
GGTGACGAGG AACAAATTGT TGCAAAGTTG TGTGACCTTG AAGACTTTGT GGAATCCCTT 
AAAAAGGATC AGAGCACATC CCAGAGGTCG ATCACCCTAA AGGACGTTGA GGATCGGGCC 
TTCATGCTGC GCCAGATAGG AGAGGCTGTT TCCAACCTGA AAGGAGAGTT TCCGTACCTC 
CAGAATAAGA TGCGGGCCGT CTTGCGTGTG GAGGTGGAGG CCGTCAGATT TCTGAAAGAG 
GAGCCCCACA AACTGGACAG CTTACTGAAA AGGATCCGAA ACATGACAGA TGCCCTTTCT 
GCCTTAAGAA GACACGCCAC TGACGGATCC TCCAAATCCA TTGACAACAA TCAGACCAGC 
CAGGTTCTAA TAACAGAAAG AGTAACTGAG ACTGATTCAC TCCAGGTCCA TGAGGAAAAG 
TCCCAAGTGT ACCTTCAGCC AACACAAACT GATGGAGGAG CAGCAGTGGA ATCGCCGACC 
ACATCTGTTA AATCGGAAGT GGTTCCCCTG TCGGCCGGCC TCAGAGTCCA TCAAGTTCAA 
AGTTCTCCGG TTCTCATGCA GCAGTCCCAG CACTCGTCTG CACTCGTGAA CCACACTCCT 
GCCCCCAGTA CCCAGAGCAG TCACAGCACA GAATTACTAG TGAGCCATCA AACTGCCAGC 
GTGCCACCCA GCGTCACCCC AGAAAGTGCA AGGAGGAACC CACCGACGGC CGCACAGGAA 
GGATCTTCCA AGCAAAGTCT TTTTATTGAG GAAATGAGTC AAGTGACTTA CAGGAACCGA 
GCGGTGTCCA TTGAGGAGGC AGAGAGAAAA TTTGAAGAGA AGAGACAGAA TCTTGATCAT 
TATAACGGAA AGGAATTTGA GAAAATGCTG GAAGAAGCAC AAGCCAGTAT CATGAAGGCC 
ATTCCCAGCT TAGAGGTGCC AGCCCAGTCC CAGAAAGTGG AGATTGTGGA CAAAAAAGAG 
GTCACGGAGG TAAAAACCAT AATGGAGCAG GATAACGATA AAGTAACAAA GTCTCCCCCT 
CCACCACCAC CTCGTCGTAT GTACCCACCA GGATCTGGAC TGAACAGTGT ATGGACAGCA 
GAATCAACGT TCTTGGTCAG GAAGGACAGC AGCAAGGATT CGGAGGAAGC CCTTCCTAAG 
GTGCCATCAA AGGCAGAAAC TGAAGAGCCT CATCTTCAGT CGGTGGAGGC TACAGTCAAG 
TCTTCTGCCG TAAAGGATGA GGATGAAGAA GAGGAAGGAG CAAGGATTAT GGCTGAACTT 
CAGGCCTTCC AGAAGTGTTC TTTTGTGGAT GTAAATCCTA AAAGCCCAAA TGAGCTTTCT 
AAAGCAGATA GCCAAGATTA CAGAGCTACA GCTGTAACCC AAGCTAAAGA GAAGAAGGTT 
TCAAAAGAGG GTTTAAATGC TGAAAAGCCA GAAGAAAAGT GGAAAATACA AAATCCAGCA 
TCTGAATCTG CAAAAATTAG GCAGGGAGAT ACTGTAATTG TGAATGAGAC CTTCAGTCTG 
TTTACAGGAA GGGAGAAATA TGGTGAGTGC CAAACTGATA AAGAGATCTT ACCTGCCCAG 
GAATGTGAGA AAGGAGATGG GTCCCCACTT GTGAGCCCAG CAATCCTTGA GAAGAGACTC 
ATCAGTTTTA GCACATCAAA AGAAACAGAG AGTGGGATCC CTCAACCCAA AAGAGAAGTG 
TTAAATGTCA AAGTTGGGGA AATTTTGCAT GTAGAAAAGA AGGAAGAGGT GCTAGAAAGT 
CACATGACAG CTTTAAAGTC ATTAAATGAG CATGAAATGC CTTATATGGA CATGGGCCAA 
ACTGTTGTAT TGAGACCTAA GTCATCGAGA AGAAGTCTTA GTCAACAACA GTCTGATGAC 
TCCAATTCTC CAACTACGTC ACCGACTGAA ACAACATCTC CAGCAGATAA TATTGCGTTC 
ATGATAACTA AAACTAAAGT CCAGGTGCTT TCAACTGGGG AGGTTCAGGA CATTGTCAGC 
CGTAAAGGAG AAGATGTACA AACCTTCAAC ATTGATAGAG GGAATAACGA GAAAATACTC 
TCTGGGAATT TAGAAAACAT TGGATCTGAA GACCCAGTGG TCTGCACTGA CAAGAAACCA 
GTCATCATCA TATTTGATGA ACCAATGGAT ATTAAATCAG CATATAAGAG GCTTTCAACA 
ATATTTGAAG AAGGTGATGA CGATCTAGAA AAACACATGT CAGAGGCTAA AATAGACGAA 
GAAGATGAAG AAGAGGAAGC AGAAGTTCCT TCACCCCAAG AATCGAAAAA GGAGTTACCA 
ATGGAACATA CCACAAAAGC ATCCTCGGAG AATCCAGGTT TGGAAAACAA GTACAGATTC 
AACTATCCAA CTTTAGCCGA AATGAAAAAG ACCATCTTCG AAGACGCCAA TGCCATACAG 
ACCCATTTCA CAGAGGAAGA CAGGTCTGAA GCTTCCGATG ATCAGCTTGG TCAAAGGCAG 
GATGTCAAGA AAAAATTCAA GTTTAAATTT CCCAAAAAAC AGCTCGCTGC GCTGACTCAA 
GCAATCCGGA CAGGGACAAA AACTGGTAAG AAAACATTAC AGGTTGTGGT CTACGAAGAG 
GAAGAAGAAG CAGATGGGAC TATAAAACAA CACAAAGAAG CAAAGAGATT TGAAATTGCA 
AGATCAAGTA GCAAAGAGGA CAATGCCAAA TACGGCGCAG AAGCAGTGGG ACTTGAGACG 
GACATTCAGG CGTCACGGAC AGATGAGATT AAAAGAACCA CCTACAAGAT TCTAGACAGT 
CTTGAGCAAA CCATAAAGCA GCAGGAAAAC CTGATGAGCG AAATGGCACC CATAATAGGG 
GCAGAGGCTT TAGAATCTAG AGAGAAATGT AAAGTCTCCA GTGACATCAA GACTGGTGCA 
GCGGAACCAG TGGAAGAACC AAAACCTGTT GTAGAGACTC CTTCCTCCAT CCCTACTGCA 
GCACGTAAGG GTTCCAGTGG AGGAGCACAA ACCAGCAGAA TGCCAATCCC AATGTCTTCT 
AAAAGTAGGC AAGCAAACGT GGACAAAAGT AGCAAACAGC CCAAACTGCA GGAGCCGCAG 
CGCCAATACA GACAGGCTAA CGGAGGTACT AAGAAAGCTG GTGGGGATGT TAAGGCTTCT 
GCCCCTTCTT TATCTGTTTC TAAGATTCCA GGCCTTTCCT CTAACACTGG GAAAAGTGGC 
TCTGTGCCTG CTCAGTCTAG CGAGACCTCT AACCCCCCTA ATCCCTCTGT CAAGTCACAT 
GTTGCCAATT CTAACCCCCA AGCTAGCCGC ACCGCTAACC CTTCAGCCCT TGTACGTCCT 
GCTCACAATG GCTCATCCAA ACTTCAGCCC CCGCCTACAT ATAAAGGTCA CCATCTTTCA 
TTCTCACCGC AGGCCTCAAA TGGCAGACCA GCTCTTCCCT CTACTACTGC TTCTAGTTCT 
TCTCCCCCTT CTTCTGTCTC TCCCACTTCA CTGACTCAAG GTGTGAAGAG CATCCGCACA 
ATCCATACAC CTAGCTTCAC CAGCTACAAG TCACAGAACG GAAGTGCAAG CAAAGGCCTG 
AATGCCAAGG AGACCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_031903539.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759399.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.

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CloneID	OXa59883
Clone ID Related Accession (Same CDS sequence)	XM_031903539.1
Accession Version	XM_031903539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACGAG ATATTTCCTA CTCGGTTCGG GAAGGCCCGG CGATCCATAG GCCTGGCTCT 
GCTACATACC CCTCCCATGC TGGCCCAGTG TCACCCCCTC CCACACCTGT TCCCCACTCC 
ATGCCATCAT CTCCATCAAG AATCCCCTAT GGCGGTGGGA GGCCTGCAGT GGTACAGAGC 
AACACCATGC AGAGGGAAAG AATACCAAGC GTGCCGGCAC CTAGATCCAT ATCTCCTAGC 
CCCAGTGCTA TTTTGGAAAG GAGAGACGTA AAACCAGATG AGGACATGGG CAATAAAAAT 
GTCCAGCTGC TAAGGAATGA CAGCATTTAC CAAGACCCTT ATTTATACCA TGAAGGACGG 
ATGAGTATGG CATCATCACA TGGTGGGCAC CCTCTCGACG TCCCTGATCA TATTATTGCA 
TACCACAGAG GGTCCTTGAG ATCTGCAAGC ACTTATTCTA ATTCTTCTAT GCAAATGGAG 
ATGATGGAAC AATCCATGTA CAGACAAAAA AGCCGTAAGC ACGTAGAGAG TCACTTACCG 
ACACTTGGTT CTAAAACTCC CCCAACCTCT CCTCACAGAG TGGCTGATAT AAGGATGATG 
GAGATGCATG CCCATAATGC CCATCTGCCT CCCCACGGCA TGCAGGCCGA CAGATCTTCT 
CCTCTCCGTC AGTCATTTAA AAAGGAGCAA GGGCCTGGCA TGTTTGTAGA AGCTAAAGTG 
CGAAACACAG GTGGAATAAT GGGCATGGCG GACATTGTCC CATCTCCTAC CGATAAGCAA 
GTATTTGGAG GGTATGGAGT AGCTGTGCCA CCAAAAGACC CCTATACAAG AGAGAGGATG 
CATGCCATGG AGAAACAGAT TGCCAGTTTA ACTGGTCTTG TTCAGACTGC ACTTTTGAAA 
GGGCCCAGCC CAAACAACTC TGCACCTAGC GACAGAGTCT TAAAATCAGC CAACGGCAGC 
GCTTCAAGCT CAGATCGGCC AGGAAATCAA GGTGTTGCTG CTGCAAAGAA TTCATTGGCC 
ATTTCAGAAA CAATAACTGT TATCAACCAG CAGTCGTCTA CCTGCTCGCA GGAAATGCAC 
GTCAGCCTAC AGGACATGAG GCGCAATGTG GCCGAACTTC GTCTTCAGCT GCATAAGATG 
AGACAACTGC AGCTGCAGAA CCAGGAGACG GTGAGGCTGA TGATGAAGAA AGCGGAGATG 
GAAATCAGCA GCAAAGTGAC TGAGATGGTC AAGGGCCTGG AGGACCCGGT GCAGAGACAG 
CGCGTCTTGG TGGAACAAGA GCGCCAGAAG TACCTCGGTG ACGAGGAACA AATTGTTGCA 
AAGTTGTGTG ACCTTGAAGA CTTTGTGGAA TCCCTTAAAA AGGATCAGAG CACATCCCAG 
AGGTCGATCA CCCTAAAGGA CGTTGAGGAT CGGGCCTTCA TGCTGCGCCA GATAGGAGAG 
GCTGTTTCCA ACCTGAAAGG AGAGTTTCCG TACCTCCAGA ATAAGATGCG GGCCGTCTTG 
CGTGTGGAGG TGGAGGCCGT CAGATTTCTG AAAGAGGAGC CCCACAAACT GGACAGCTTA 
CTGAAAAGGA TCCGAAACAT GACAGATGCC CTTTCTGCCT TAAGAAGACA CGCCACTGAC 
GGATCCTCCA AATCCATTGA CAACAATCAG ACCAGCCAGG TTCTAATAAC AGAAAGAGTA 
ACTGAGACTG ATTCACTCCA GGTCCATGAG GAAAAGTCCC AAGTGTACCT TCAGCCAACA 
CAAACTGATG GAGGAGCAGC AGTGGAATCG CCGACCACAT CTGTTAAATC GGAAGTGGTT 
CCCCTGTCGG CCGGCCTCAG AGTCCATCAA GTTCAAAGTT CTCCGGTTCT CATGCAGCAG 
TCCCAGCACT CGTCTGCACT CGTGAACCAC ACTCCTGCCC CCAGTACCCA GAGCAGTCAC 
AGCACAGAAT TACTAGTGAG CCATCAAACT GCCAGCGTGC CACCCAGCGT CACCCCAGAA 
AGTGCAAGGA GGAACCCACC GACGGCCGCA CAGGAAGGAT CTTCCAAGCA AAGTCTTTTT 
ATTGAGGAAA TGAGTCAAGT GACTTACAGG AACCGAGCGG TGTCCATTGA GGAGGCAGAG 
AGAAAATTTG AAGAGAAGAG ACAGAATCTT GATCATTATA ACGGAAAGGA ATTTGAGAAA 
ATGCTGGAAG AAGCACAAGC CAGTATCATG AAGGCCATTC CCAGCTTAGA GGTGCCAGCC 
CAGTCCCAGA AAGTGGAGAT TGTGGACAAA AAAGAGGTCA CGGAGGTAAA AACCATAATG 
GAGCAGGATA ACGATAAAGT AACAAAGTCT CCCCCTCCAC CACCACCTCG TCGTATGTAC 
CCACCAGGAT CTGGACTGAA CAGTGTATGG ACAGCAGAAT CAACGTTCTT GGTCAGGAAG 
GACAGCAGCA AGGATTCGGA GGAAGCCCTT CCTAAGGTGC CATCAAAGGC AGAAACTGAA 
GAGCCTCATC TTCAGTCGGT GGAGGCTACA GTCAAGTCTT CTGCCGTAAA GGATGAGGAT 
GAAGAAGAGG AAGGAGCAAG GATTATGGCT GAACTTCAGG CCTTCCAGAA GTGTTCTTTT 
GTGGATGTAA ATCCTAAAAG CCCAAATGAG CTTTCTAAAG CAGATAGCCA AGATTACAGA 
GCTACAGCTG TAACCCAAGC TAAAGAGAAG AAGGTTTCAA AAGAGGGTTT AAATGCTGAA 
AAGCCAGAAG AAAAGTGGAA AATACAAAAT CCAGCATCTG AATCTGCAAA AATTAGGCAG 
GGAGATACTG TAATTGTGAA TGAGACCTTC AGTCTGTTTA CAGGAAGGGA GAAATATGGT 
GAGTGCCAAA CTGATAAAGA GATCTTACCT GCCCAGGAAT GTGAGAAAGG AGATGGGTCC 
CCACTTGTGA GCCCAGCAAT CCTTGAGAAG AGACTCATCA GTTTTAGCAC ATCAAAAGAA 
ACAGAGAGTG GGATCCCTCA ACCCAAAAGA GAAGTGTTAA ATGTCAAAGT TGGGGAAATT 
TTGCATGTAG AAAAGAAGGA AGAGGTGCTA GAAAGTCACA TGACAGCTTT AAAGTCATTA 
AATGAGCATG AAATGCCTTA TATGGACATG GGCCAAACTG TTGTATTGAG ACCTAAGTCA 
TCGAGAAGAA GTCTTAGTCA ACAACAGTCT GATGACTCCA ATTCTCCAAC TACGTCACCG 
ACTGAAACAA CATCTCCAGC AGATAATATT GCGTTCATGA TAACTAAAAC TAAAGTCCAG 
GTGCTTTCAA CTGGGGAGGT TCAGGACATT GTCAGCCGTA AAGGAGAAGA TGTACAAACC 
TTCAACATTG ATAGAGGGAA TAACGAGAAA ATACTCTCTG GGAATTTAGA AAACATTGGA 
TCTGAAGACC CAGTGGTCTG CACTGACAAG AAACCAGTCA TCATCATATT TGATGAACCA 
ATGGATATTA AATCAGCATA TAAGAGGCTT TCAACAATAT TTGAAGAAGG TGATGACGAT 
CTAGAAAAAC ACATGTCAGA GGCTAAAATA GACGAAGAAG ATGAAGAAGA GGAAGCAGAA 
GTTCCTTCAC CCCAAGAATC GAAAAAGGAG TTACCAATGG AACATACCAC AAAAGCATCC 
TCGGAGAATC CAGGTTTGGA AAACAAGTAC AGATTCAACT ATCCAACTTT AGCCGAAATG 
AAAAAGACCA TCTTCGAAGA CGCCAATGCC ATACAGACCC ATTTCACAGA GGAAGACAGG 
TCTGAAGCTT CCGATGATCA GCTTGGTCAA AGGCAGGATG TCAAGAAAAA ATTCAAGTTT 
AAATTTCCCA AAAAACAGCT CGCTGCGCTG ACTCAAGCAA TCCGGACAGG GACAAAAACT 
GGTAAGAAAA CATTACAGGT TGTGGTCTAC GAAGAGGAAG AAGAAGCAGA TGGGACTATA 
AAACAACACA AAGAAGCAAA GAGATTTGAA ATTGCAAGAT CAAGTAGCAA AGAGGACAAT 
GCCAAATACG GCGCAGAAGC AGTGGGACTT GAGACGGACA TTCAGGCGTC ACGGACAGAT 
GAGATTAAAA GAACCACCTA CAAGATTCTA GACAGTCTTG AGCAAACCAT AAAGCAGCAG 
GAAAACCTGA TGAGCGAAAT GGCACCCATA ATAGGGGCAG AGGCTTTAGA ATCTAGAGAG 
AAATGTAAAG TCTCCAGTGA CATCAAGACT GGTGCAGCGG AACCAGTGGA AGAACCAAAA 
CCTGTTGTAG AGACTCCTTC CTCCATCCCT ACTGCAGCAC GTAAGGGTTC CAGTGGAGGA 
GCACAAACCA GCAGAATGCC AATCCCAATG TCTTCTAAAA GTAGGCAAGC AAACGTGGAC 
AAAAGTAGCA AACAGCCCAA ACTGCAGGAG CCGCAGCGCC AATACAGACA GGCTAACGGA 
GGTACTAAGA AAGCTGGTGG GGATGTTAAG GCTTCTGCCC CTTCTTTATC TGTTTCTAAG 
ATTCCAGGCC TTTCCTCTAA CACTGGGAAA AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG 
ACCTCTAACC CCCCTAATCC CTCTGTCAAG TCACATGTTG CCAATTCTAA CCCCCAAGCT 
AGCCGCACCG CTAACCCTTC AGCCCTTGTA CGTCCTGCTC ACAATGGCTC ATCCAAACTT 
CAGCCCCCGC CTACATATAA AGGTCACCAT CTTTCATTCT CACCGCAGGC CTCAAATGGC 
AGACCAGCTC TTCCCTCTAC TACTGCTTCT AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC 
ACTTCACTGA CTCAAGGTGT GAAGAGCATC CGCACAATCC ATACACCTAG CTTCACCAGC 
TACAAGTCAC AGAACGGAAG TGCAAGCAAA GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759399.1
CDS	91..4947
Translation	MQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_031903539.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903539.1

ORF Insert Sequence:

ATGCAACGAG ATATTTCCTA CTCGGTTCGG GAAGGCCCGG CGATCCATAG GCCTGGCTCT 
GCTACATACC CCTCCCATGC TGGCCCAGTG TCACCCCCTC CCACACCTGT TCCCCACTCC 
ATGCCATCAT CTCCATCAAG AATCCCCTAT GGCGGTGGGA GGCCTGCAGT GGTACAGAGC 
AACACCATGC AGAGGGAAAG AATACCAAGC GTGCCGGCAC CTAGATCCAT ATCTCCTAGC 
CCCAGTGCTA TTTTGGAAAG GAGAGACGTA AAACCAGATG AGGACATGGG CAATAAAAAT 
GTCCAGCTGC TAAGGAATGA CAGCATTTAC CAAGACCCTT ATTTATACCA TGAAGGACGG 
ATGAGTATGG CATCATCACA TGGTGGGCAC CCTCTCGACG TCCCTGATCA TATTATTGCA 
TACCACAGAG GGTCCTTGAG ATCTGCAAGC ACTTATTCTA ATTCTTCTAT GCAAATGGAG 
ATGATGGAAC AATCCATGTA CAGACAAAAA AGCCGTAAGC ACGTAGAGAG TCACTTACCG 
ACACTTGGTT CTAAAACTCC CCCAACCTCT CCTCACAGAG TGGCTGATAT AAGGATGATG 
GAGATGCATG CCCATAATGC CCATCTGCCT CCCCACGGCA TGCAGGCCGA CAGATCTTCT 
CCTCTCCGTC AGTCATTTAA AAAGGAGCAA GGGCCTGGCA TGTTTGTAGA AGCTAAAGTG 
CGAAACACAG GTGGAATAAT GGGCATGGCG GACATTGTCC CATCTCCTAC CGATAAGCAA 
GTATTTGGAG GGTATGGAGT AGCTGTGCCA CCAAAAGACC CCTATACAAG AGAGAGGATG 
CATGCCATGG AGAAACAGAT TGCCAGTTTA ACTGGTCTTG TTCAGACTGC ACTTTTGAAA 
GGGCCCAGCC CAAACAACTC TGCACCTAGC GACAGAGTCT TAAAATCAGC CAACGGCAGC 
GCTTCAAGCT CAGATCGGCC AGGAAATCAA GGTGTTGCTG CTGCAAAGAA TTCATTGGCC 
ATTTCAGAAA CAATAACTGT TATCAACCAG CAGTCGTCTA CCTGCTCGCA GGAAATGCAC 
GTCAGCCTAC AGGACATGAG GCGCAATGTG GCCGAACTTC GTCTTCAGCT GCATAAGATG 
AGACAACTGC AGCTGCAGAA CCAGGAGACG GTGAGGCTGA TGATGAAGAA AGCGGAGATG 
GAAATCAGCA GCAAAGTGAC TGAGATGGTC AAGGGCCTGG AGGACCCGGT GCAGAGACAG 
CGCGTCTTGG TGGAACAAGA GCGCCAGAAG TACCTCGGTG ACGAGGAACA AATTGTTGCA 
AAGTTGTGTG ACCTTGAAGA CTTTGTGGAA TCCCTTAAAA AGGATCAGAG CACATCCCAG 
AGGTCGATCA CCCTAAAGGA CGTTGAGGAT CGGGCCTTCA TGCTGCGCCA GATAGGAGAG 
GCTGTTTCCA ACCTGAAAGG AGAGTTTCCG TACCTCCAGA ATAAGATGCG GGCCGTCTTG 
CGTGTGGAGG TGGAGGCCGT CAGATTTCTG AAAGAGGAGC CCCACAAACT GGACAGCTTA 
CTGAAAAGGA TCCGAAACAT GACAGATGCC CTTTCTGCCT TAAGAAGACA CGCCACTGAC 
GGATCCTCCA AATCCATTGA CAACAATCAG ACCAGCCAGG TTCTAATAAC AGAAAGAGTA 
ACTGAGACTG ATTCACTCCA GGTCCATGAG GAAAAGTCCC AAGTGTACCT TCAGCCAACA 
CAAACTGATG GAGGAGCAGC AGTGGAATCG CCGACCACAT CTGTTAAATC GGAAGTGGTT 
CCCCTGTCGG CCGGCCTCAG AGTCCATCAA GTTCAAAGTT CTCCGGTTCT CATGCAGCAG 
TCCCAGCACT CGTCTGCACT CGTGAACCAC ACTCCTGCCC CCAGTACCCA GAGCAGTCAC 
AGCACAGAAT TACTAGTGAG CCATCAAACT GCCAGCGTGC CACCCAGCGT CACCCCAGAA 
AGTGCAAGGA GGAACCCACC GACGGCCGCA CAGGAAGGAT CTTCCAAGCA AAGTCTTTTT 
ATTGAGGAAA TGAGTCAAGT GACTTACAGG AACCGAGCGG TGTCCATTGA GGAGGCAGAG 
AGAAAATTTG AAGAGAAGAG ACAGAATCTT GATCATTATA ACGGAAAGGA ATTTGAGAAA 
ATGCTGGAAG AAGCACAAGC CAGTATCATG AAGGCCATTC CCAGCTTAGA GGTGCCAGCC 
CAGTCCCAGA AAGTGGAGAT TGTGGACAAA AAAGAGGTCA CGGAGGTAAA AACCATAATG 
GAGCAGGATA ACGATAAAGT AACAAAGTCT CCCCCTCCAC CACCACCTCG TCGTATGTAC 
CCACCAGGAT CTGGACTGAA CAGTGTATGG ACAGCAGAAT CAACGTTCTT GGTCAGGAAG 
GACAGCAGCA AGGATTCGGA GGAAGCCCTT CCTAAGGTGC CATCAAAGGC AGAAACTGAA 
GAGCCTCATC TTCAGTCGGT GGAGGCTACA GTCAAGTCTT CTGCCGTAAA GGATGAGGAT 
GAAGAAGAGG AAGGAGCAAG GATTATGGCT GAACTTCAGG CCTTCCAGAA GTGTTCTTTT 
GTGGATGTAA ATCCTAAAAG CCCAAATGAG CTTTCTAAAG CAGATAGCCA AGATTACAGA 
GCTACAGCTG TAACCCAAGC TAAAGAGAAG AAGGTTTCAA AAGAGGGTTT AAATGCTGAA 
AAGCCAGAAG AAAAGTGGAA AATACAAAAT CCAGCATCTG AATCTGCAAA AATTAGGCAG 
GGAGATACTG TAATTGTGAA TGAGACCTTC AGTCTGTTTA CAGGAAGGGA GAAATATGGT 
GAGTGCCAAA CTGATAAAGA GATCTTACCT GCCCAGGAAT GTGAGAAAGG AGATGGGTCC 
CCACTTGTGA GCCCAGCAAT CCTTGAGAAG AGACTCATCA GTTTTAGCAC ATCAAAAGAA 
ACAGAGAGTG GGATCCCTCA ACCCAAAAGA GAAGTGTTAA ATGTCAAAGT TGGGGAAATT 
TTGCATGTAG AAAAGAAGGA AGAGGTGCTA GAAAGTCACA TGACAGCTTT AAAGTCATTA 
AATGAGCATG AAATGCCTTA TATGGACATG GGCCAAACTG TTGTATTGAG ACCTAAGTCA 
TCGAGAAGAA GTCTTAGTCA ACAACAGTCT GATGACTCCA ATTCTCCAAC TACGTCACCG 
ACTGAAACAA CATCTCCAGC AGATAATATT GCGTTCATGA TAACTAAAAC TAAAGTCCAG 
GTGCTTTCAA CTGGGGAGGT TCAGGACATT GTCAGCCGTA AAGGAGAAGA TGTACAAACC 
TTCAACATTG ATAGAGGGAA TAACGAGAAA ATACTCTCTG GGAATTTAGA AAACATTGGA 
TCTGAAGACC CAGTGGTCTG CACTGACAAG AAACCAGTCA TCATCATATT TGATGAACCA 
ATGGATATTA AATCAGCATA TAAGAGGCTT TCAACAATAT TTGAAGAAGG TGATGACGAT 
CTAGAAAAAC ACATGTCAGA GGCTAAAATA GACGAAGAAG ATGAAGAAGA GGAAGCAGAA 
GTTCCTTCAC CCCAAGAATC GAAAAAGGAG TTACCAATGG AACATACCAC AAAAGCATCC 
TCGGAGAATC CAGGTTTGGA AAACAAGTAC AGATTCAACT ATCCAACTTT AGCCGAAATG 
AAAAAGACCA TCTTCGAAGA CGCCAATGCC ATACAGACCC ATTTCACAGA GGAAGACAGG 
TCTGAAGCTT CCGATGATCA GCTTGGTCAA AGGCAGGATG TCAAGAAAAA ATTCAAGTTT 
AAATTTCCCA AAAAACAGCT CGCTGCGCTG ACTCAAGCAA TCCGGACAGG GACAAAAACT 
GGTAAGAAAA CATTACAGGT TGTGGTCTAC GAAGAGGAAG AAGAAGCAGA TGGGACTATA 
AAACAACACA AAGAAGCAAA GAGATTTGAA ATTGCAAGAT CAAGTAGCAA AGAGGACAAT 
GCCAAATACG GCGCAGAAGC AGTGGGACTT GAGACGGACA TTCAGGCGTC ACGGACAGAT 
GAGATTAAAA GAACCACCTA CAAGATTCTA GACAGTCTTG AGCAAACCAT AAAGCAGCAG 
GAAAACCTGA TGAGCGAAAT GGCACCCATA ATAGGGGCAG AGGCTTTAGA ATCTAGAGAG 
AAATGTAAAG TCTCCAGTGA CATCAAGACT GGTGCAGCGG AACCAGTGGA AGAACCAAAA 
CCTGTTGTAG AGACTCCTTC CTCCATCCCT ACTGCAGCAC GTAAGGGTTC CAGTGGAGGA 
GCACAAACCA GCAGAATGCC AATCCCAATG TCTTCTAAAA GTAGGCAAGC AAACGTGGAC 
AAAAGTAGCA AACAGCCCAA ACTGCAGGAG CCGCAGCGCC AATACAGACA GGCTAACGGA 
GGTACTAAGA AAGCTGGTGG GGATGTTAAG GCTTCTGCCC CTTCTTTATC TGTTTCTAAG 
ATTCCAGGCC TTTCCTCTAA CACTGGGAAA AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG 
ACCTCTAACC CCCCTAATCC CTCTGTCAAG TCACATGTTG CCAATTCTAA CCCCCAAGCT 
AGCCGCACCG CTAACCCTTC AGCCCTTGTA CGTCCTGCTC ACAATGGCTC ATCCAAACTT 
CAGCCCCCGC CTACATATAA AGGTCACCAT CTTTCATTCT CACCGCAGGC CTCAAATGGC 
AGACCAGCTC TTCCCTCTAC TACTGCTTCT AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC 
ACTTCACTGA CTCAAGGTGT GAAGAGCATC CGCACAATCC ATACACCTAG CTTCACCAGC 
TACAAGTCAC AGAACGGAAG TGCAAGCAAA GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915435.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915492.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.

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CloneID	OXa12038
Clone ID Related Accession (Same CDS sequence)	XM_004915435.3 , XM_004915435.4
Accession Version	XM_004915435.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGTAAGGGT TCCAGTGGAG GAGCACAAAC CAGCAGAATG 
CCAATCCCAA TGTCTTCTAA AAGTAGGCAA GCAAACGTGG ACAAAAGTAG CAAACAGCCC 
AAACTGCAGG AGCCGCAGCG CCAATACAGA CAGGCTAACG GAGGTACTAA GAAAGCTGGT 
GGGGATGTTA AGGCTTCTGC CCCTTCTTTA TCTGTTTCTA AGATTCCAGG CCTTTCCTCT 
AACACTGGGA AAAGTGGCTC TGTGCCTGCT CAGTCTAGCG AGACCTCTAA CCCCCCTAAT 
CCCTCTGTCA AGTCACATGT TGCCAATTCT AACCCCCAAG CTAGCCGCAC CGCTAACCCT 
TCAGCCCTTG TACGTCCTGC TCACAATGGC TCATCCAAAC TTCAGCCCCC GCCTACATAT 
AAAGGTCACC ATCTTTCATT CTCACCGCAG GCCTCAAATG GCAGACCAGC TCTTCCCTCT 
ACTACTGCTT CTAGTTCTTC TCCCCCTTCT TCTGTCTCTC CCACTTCACT GACTCAAGGT 
GTGAAGAGCA TCCGCACAAT CCATACACCT AGCTTCACCA GCTACAAGTC ACAGAACGGA 
AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915492.1
CDS	292..6210
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915435.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915435.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGTAAGGGT TCCAGTGGAG GAGCACAAAC CAGCAGAATG 
CCAATCCCAA TGTCTTCTAA AAGTAGGCAA GCAAACGTGG ACAAAAGTAG CAAACAGCCC 
AAACTGCAGG AGCCGCAGCG CCAATACAGA CAGGCTAACG GAGGTACTAA GAAAGCTGGT 
GGGGATGTTA AGGCTTCTGC CCCTTCTTTA TCTGTTTCTA AGATTCCAGG CCTTTCCTCT 
AACACTGGGA AAAGTGGCTC TGTGCCTGCT CAGTCTAGCG AGACCTCTAA CCCCCCTAAT 
CCCTCTGTCA AGTCACATGT TGCCAATTCT AACCCCCAAG CTAGCCGCAC CGCTAACCCT 
TCAGCCCTTG TACGTCCTGC TCACAATGGC TCATCCAAAC TTCAGCCCCC GCCTACATAT 
AAAGGTCACC ATCTTTCATT CTCACCGCAG GCCTCAAATG GCAGACCAGC TCTTCCCTCT 
ACTACTGCTT CTAGTTCTTC TCCCCCTTCT TCTGTCTCTC CCACTTCACT GACTCAAGGT 
GTGAAGAGCA TCCGCACAAT CCATACACCT AGCTTCACCA GCTACAAGTC ACAGAACGGA 
AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_002933147.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933193.2 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.

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CloneID	OXa12039
Clone ID Related Accession (Same CDS sequence)	XM_002933147.5 , XM_002933147.4
Accession Version	XM_002933147.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933147.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGTTTCA 
AAAGAGGGTT TAAATGCTGA AAAGCCAGAA GAAAAGTGGA AAATACAAAA TCCAGCATCT 
GAATCTGCAA AAATTAGGCA GGGAGATACT GTAATTGTGA ATGAGACCTT CAGTCTGTTT 
ACAGGAAGGG AGAAATATGG TGAGTGCCAA ACTGATAAAG AGATCTTACC TGCCCAGGAA 
TGTGAGAAAG GAGATGGGTC CCCACTTGTG AGCCCAGCAA TCCTTGAGAA GAGACTCATC 
AGTTTTAGCA CATCAAAAGA AACAGAGAGT GGGATCCCTC AACCCAAAAG AGAAGTGTTA 
AATGTCAAAG TTGGGGAAAT TTTGCATGTA GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC 
ATGACAGCTT TAAAGTCATT AAATGAGCAT GAAATGCCTT ATATGGACAT GGGCCAAACT 
GTTGTATTGA GACCTAAGTC ATCGAGAAGA AGTCTTAGTC AACAACAGTC TGATGACTCC 
AATTCTCCAA CTACGTCACC GACTGAAACA ACATCTCCAG CAGATAATAT TGCGTTCATG 
ATAACTAAAA CTAAAGTCCA GGTGCTTTCA ACTGGGGAGG TTCAGGACAT TGTCAGCCGT 
AAAGGAGAAG ATGTACAAAC CTTCAACATT GATAGAGGGA ATAACGAGAA AATACTCTCT 
GGGAATTTAG AAAACATTGG ATCTGAAGAC CCAGTGGTCT GCACTGACAA GAAACCAGTC 
ATCATCATAT TTGATGAACC AATGGATATT AAATCAGCAT ATAAGAGGCT TTCAACAATA 
TTTGAAGAAG GTGATGACGA TCTAGAAAAA CACATGTCAG AGGCTAAAAT AGACGAAGAA 
GATGAAGAAG AGGAAGCAGA AGTTCCTTCA CCCCAAGAAT CGAAAAAGGA GTTACCAATG 
GAACATACCA CAAAAGCATC CTCGGAGAAT CCAGGTTTGG AAAACAAGTA CAGATTCAAC 
TATCCAACTT TAGCCGAAAT GAAAAAGACC ATCTTCGAAG ACGCCAATGC CATACAGACC 
CATTTCACAG AGGAAGACAG GTCTGAAGCT TCCGATGATC AGCTTGGTCA AAGGCAGGAT 
GTCAAGAAAA AATTCAAGTT TAAATTTCCC AAAAAACAGC TCGCTGCGCT GACTCAAGCA 
ATCCGGACAG GGACAAAAAC TGGTAAGAAA ACATTACAGG TTGTGGTCTA CGAAGAGGAA 
GAAGAAGCAG ATGGGACTAT AAAACAACAC AAAGAAGCAA AGAGATTTGA AATTGCAAGA 
TCAAGTAGCA AAGAGGACAA TGCCAAATAC GGCGCAGAAG CAGTGGGACT TGAGACGGAC 
ATTCAGGCGT CACGGACAGA TGAGATTAAA AGAACCACCT ACAAGATTCT AGACAGTCTT 
GAGCAAACCA TAAAGCAGCA GGAAAACCTG ATGAGCGAAA TGGCACCCAT AATAGGGGCA 
GAGGCTTTAG AATCTAGAGA GAAATGTAAA GTCTCCAGTG ACATCAAGAC TGGTGCAGCG 
GAACCAGTGG AAGAACCAAA ACCTGTTGTA GAGACTCCTT CCTCCATCCC TACTGCAGCA 
CGTAAGGGTT CCAGTGGAGG AGCACAAACC AGCAGAATGC CAATCCCAAT GTCTTCTAAA 
AGTAGGCAAG CAAACGTGGA CAAAAGTAGC AAACAGCCCA AACTGCAGGA GCCGCAGCGC 
CAATACAGAC AGGCTAACGG AGGTACTAAG AAAGCTGGTG GGGATGTTAA GGCTTCTGCC 
CCTTCTTTAT CTGTTTCTAA GATTCCAGGC CTTTCCTCTA ACACTGGGAA AAGTGGCTCT 
GTGCCTGCTC AGTCTAGCGA GACCTCTAAC CCCCCTAATC CCTCTGTCAA GTCACATGTT 
GCCAATTCTA ACCCCCAAGC TAGCCGCACC GCTAACCCTT CAGCCCTTGT ACGTCCTGCT 
CACAATGGCT CATCCAAACT TCAGCCCCCG CCTACATATA AAGGTCACCA TCTTTCATTC 
TCACCGCAGG CCTCAAATGG CAGACCAGCT CTTCCCTCTA CTACTGCTTC TAGTTCTTCT 
CCCCCTTCTT CTGTCTCTCC CACTTCACTG ACTCAAGGTG TGAAGAGCAT CCGCACAATC 
CATACACCTA GCTTCACCAG CTACAAGTCA CAGAACGGAA GTGCAAGCAA AGGCCTGAAT 
GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933193.2
CDS	291..6128
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_002933147.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933147.4

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGTTTCA 
AAAGAGGGTT TAAATGCTGA AAAGCCAGAA GAAAAGTGGA AAATACAAAA TCCAGCATCT 
GAATCTGCAA AAATTAGGCA GGGAGATACT GTAATTGTGA ATGAGACCTT CAGTCTGTTT 
ACAGGAAGGG AGAAATATGG TGAGTGCCAA ACTGATAAAG AGATCTTACC TGCCCAGGAA 
TGTGAGAAAG GAGATGGGTC CCCACTTGTG AGCCCAGCAA TCCTTGAGAA GAGACTCATC 
AGTTTTAGCA CATCAAAAGA AACAGAGAGT GGGATCCCTC AACCCAAAAG AGAAGTGTTA 
AATGTCAAAG TTGGGGAAAT TTTGCATGTA GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC 
ATGACAGCTT TAAAGTCATT AAATGAGCAT GAAATGCCTT ATATGGACAT GGGCCAAACT 
GTTGTATTGA GACCTAAGTC ATCGAGAAGA AGTCTTAGTC AACAACAGTC TGATGACTCC 
AATTCTCCAA CTACGTCACC GACTGAAACA ACATCTCCAG CAGATAATAT TGCGTTCATG 
ATAACTAAAA CTAAAGTCCA GGTGCTTTCA ACTGGGGAGG TTCAGGACAT TGTCAGCCGT 
AAAGGAGAAG ATGTACAAAC CTTCAACATT GATAGAGGGA ATAACGAGAA AATACTCTCT 
GGGAATTTAG AAAACATTGG ATCTGAAGAC CCAGTGGTCT GCACTGACAA GAAACCAGTC 
ATCATCATAT TTGATGAACC AATGGATATT AAATCAGCAT ATAAGAGGCT TTCAACAATA 
TTTGAAGAAG GTGATGACGA TCTAGAAAAA CACATGTCAG AGGCTAAAAT AGACGAAGAA 
GATGAAGAAG AGGAAGCAGA AGTTCCTTCA CCCCAAGAAT CGAAAAAGGA GTTACCAATG 
GAACATACCA CAAAAGCATC CTCGGAGAAT CCAGGTTTGG AAAACAAGTA CAGATTCAAC 
TATCCAACTT TAGCCGAAAT GAAAAAGACC ATCTTCGAAG ACGCCAATGC CATACAGACC 
CATTTCACAG AGGAAGACAG GTCTGAAGCT TCCGATGATC AGCTTGGTCA AAGGCAGGAT 
GTCAAGAAAA AATTCAAGTT TAAATTTCCC AAAAAACAGC TCGCTGCGCT GACTCAAGCA 
ATCCGGACAG GGACAAAAAC TGGTAAGAAA ACATTACAGG TTGTGGTCTA CGAAGAGGAA 
GAAGAAGCAG ATGGGACTAT AAAACAACAC AAAGAAGCAA AGAGATTTGA AATTGCAAGA 
TCAAGTAGCA AAGAGGACAA TGCCAAATAC GGCGCAGAAG CAGTGGGACT TGAGACGGAC 
ATTCAGGCGT CACGGACAGA TGAGATTAAA AGAACCACCT ACAAGATTCT AGACAGTCTT 
GAGCAAACCA TAAAGCAGCA GGAAAACCTG ATGAGCGAAA TGGCACCCAT AATAGGGGCA 
GAGGCTTTAG AATCTAGAGA GAAATGTAAA GTCTCCAGTG ACATCAAGAC TGGTGCAGCG 
GAACCAGTGG AAGAACCAAA ACCTGTTGTA GAGACTCCTT CCTCCATCCC TACTGCAGCA 
CGTAAGGGTT CCAGTGGAGG AGCACAAACC AGCAGAATGC CAATCCCAAT GTCTTCTAAA 
AGTAGGCAAG CAAACGTGGA CAAAAGTAGC AAACAGCCCA AACTGCAGGA GCCGCAGCGC 
CAATACAGAC AGGCTAACGG AGGTACTAAG AAAGCTGGTG GGGATGTTAA GGCTTCTGCC 
CCTTCTTTAT CTGTTTCTAA GATTCCAGGC CTTTCCTCTA ACACTGGGAA AAGTGGCTCT 
GTGCCTGCTC AGTCTAGCGA GACCTCTAAC CCCCCTAATC CCTCTGTCAA GTCACATGTT 
GCCAATTCTA ACCCCCAAGC TAGCCGCACC GCTAACCCTT CAGCCCTTGT ACGTCCTGCT 
CACAATGGCT CATCCAAACT TCAGCCCCCG CCTACATATA AAGGTCACCA TCTTTCATTC 
TCACCGCAGG CCTCAAATGG CAGACCAGCT CTTCCCTCTA CTACTGCTTC TAGTTCTTCT 
CCCCCTTCTT CTGTCTCTCC CACTTCACTG ACTCAAGGTG TGAAGAGCAT CCGCACAATC 
CATACACCTA GCTTCACCAG CTACAAGTCA CAGAACGGAA GTGCAAGCAA AGGCCTGAAT 
GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915436.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915493.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.

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CloneID	OXa12040
Clone ID Related Accession (Same CDS sequence)	XM_004915436.4 , XM_004915436.3
Accession Version	XM_004915436.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGTTTCAAAA GAGGGTTTAA ATGCTGAAAA GCCAGAAGAA 
AAGTGGAAAA TACAAAATCC AGCATCTGAA TCTGCAAAAA TTAGGCAGGG AGATACTGTA 
ATTGTGAATG AGACCTTCAG TCTGTTTACA GGAAGGGAGA AATATGGTGA GTGCCAAACT 
GATAAAGAGA TCTTACCTGC CCAGGAATGT GAGAAAGGAG ATGGGTCCCC ACTTGTGAGC 
CCAGCAATCC TTGAGAAGAG ACTCATCAGT TTTAGCACAT CAAAAGAAAC AGAGAGTGGG 
ATCCCTCAAC CCAAAAGAGA AGTGTTAAAT GTCAAAGTTG GGGAAATTTT GCATGTAGAA 
AAGAAGGAAG AGGTGCTAGA AAGTCACATG ACAGCTTTAA AGTCATTAAA TGAGCATGAA 
ATGCCTTATA TGGACATGGG CCAAACTGTT GTATTGAGAC CTAAGTCATC GAGAAGAAGT 
CTTAGTCAAC AACAGTCTGA TGACTCCAAT TCTCCAACTA CGTCACCGAC TGAAACAACA 
TCTCCAGCAG ATAATATTGC GTTCATGATA ACTAAAACTA AAGTCCAGGT GCTTTCAACT 
GGGGAGGTTC AGGACATTGT CAGCCGTAAA GGAGAAGATG TACAAACCTT CAACATTGAT 
AGAGGGAATA ACGAGAAAAT ACTCTCTGGG AATTTAGAAA ACATTGGATC TGAAGACCCA 
GTGGTCTGCA CTGACAAGAA ACCAGTCATC ATCATATTTG ATGAACCAAT GGATATTAAA 
TCAGCATATA AGAGGCTTTC AACAATATTT GAAGAAGGTG ATGACGATCT AGAAAAACAC 
ATGTCAGAGG CTAAAATAGA CGAAGAAGAT GAAGAAGAGG AAGCAGAAGT TCCTTCACCC 
CAAGAATCGA AAAAGGAGTT ACCAATGGAA CATACCACAA AAGCATCCTC GGAGAATCCA 
GGTTTGGAAA ACAAGTACAG ATTCAACTAT CCAACTTTAG CCGAAATGAA AAAGACCATC 
TTCGAAGACG CCAATGCCAT ACAGACCCAT TTCACAGAGG AAGACAGGTC TGAAGCTTCC 
GATGATCAGC TTGGTCAAAG GCAGGATGTC AAGAAAAAAT TCAAGTTTAA ATTTCCCAAA 
AAACAGCTCG CTGCGCTGAC TCAAGCAATC CGGACAGGGA CAAAAACTGG TAAGAAAACA 
TTACAGGTTG TGGTCTACGA AGAGGAAGAA GAAGCAGATG GGACTATAAA ACAACACAAA 
GAAGCAAAGA GATTTGAAAT TGCAAGATCA AGTAGCAAAG AGGACAATGC CAAATACGGC 
GCAGAAGCAG TGGGACTTGA GACGGACATT CAGGCGTCAC GGACAGATGA GATTAAAAGA 
ACCACCTACA AGATTCTAGA CAGTCTTGAG CAAACCATAA AGCAGCAGGA AAACCTGATG 
AGCGAAATGG CACCCATAAT AGGGGCAGAG GCTTTAGAAT CTAGAGAGAA ATGTAAAGTC 
TCCAGTGACA TCAAGACTGG TGCAGCGGAA CCAGTGGAAG AACCAAAACC TGTTGTAGAG 
ACTCCTTCCT CCATCCCTAC TGCAGCACGT AAGGGTTCCA GTGGAGGAGC ACAAACCAGC 
AGAATGCCAA TCCCAATGTC TTCTAAAAGT AGGCAAGCAA ACGTGGACAA AAGTAGCAAA 
CAGCCCAAAC TGCAGGAGCC GCAGCGCCAA TACAGACAGG CTAACGGAGG TACTAAGAAA 
GCTGGTGGGG ATGTTAAGGC TTCTGCCCCT TCTTTATCTG TTTCTAAGAT TCCAGGCCTT 
TCCTCTAACA CTGGGAAAAG TGGCTCTGTG CCTGCTCAGT CTAGCGAGAC CTCTAACCCC 
CCTAATCCCT CTGTCAAGTC ACATGTTGCC AATTCTAACC CCCAAGCTAG CCGCACCGCT 
AACCCTTCAG CCCTTGTACG TCCTGCTCAC AATGGCTCAT CCAAACTTCA GCCCCCGCCT 
ACATATAAAG GTCACCATCT TTCATTCTCA CCGCAGGCCT CAAATGGCAG ACCAGCTCTT 
CCCTCTACTA CTGCTTCTAG TTCTTCTCCC CCTTCTTCTG TCTCTCCCAC TTCACTGACT 
CAAGGTGTGA AGAGCATCCG CACAATCCAT ACACCTAGCT TCACCAGCTA CAAGTCACAG 
AACGGAAGTG CAAGCAAAGG CCTGAATGCC AAGGAGACCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915493.1
CDS	291..6095
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915436.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915436.3

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGTTTCAAAA GAGGGTTTAA ATGCTGAAAA GCCAGAAGAA 
AAGTGGAAAA TACAAAATCC AGCATCTGAA TCTGCAAAAA TTAGGCAGGG AGATACTGTA 
ATTGTGAATG AGACCTTCAG TCTGTTTACA GGAAGGGAGA AATATGGTGA GTGCCAAACT 
GATAAAGAGA TCTTACCTGC CCAGGAATGT GAGAAAGGAG ATGGGTCCCC ACTTGTGAGC 
CCAGCAATCC TTGAGAAGAG ACTCATCAGT TTTAGCACAT CAAAAGAAAC AGAGAGTGGG 
ATCCCTCAAC CCAAAAGAGA AGTGTTAAAT GTCAAAGTTG GGGAAATTTT GCATGTAGAA 
AAGAAGGAAG AGGTGCTAGA AAGTCACATG ACAGCTTTAA AGTCATTAAA TGAGCATGAA 
ATGCCTTATA TGGACATGGG CCAAACTGTT GTATTGAGAC CTAAGTCATC GAGAAGAAGT 
CTTAGTCAAC AACAGTCTGA TGACTCCAAT TCTCCAACTA CGTCACCGAC TGAAACAACA 
TCTCCAGCAG ATAATATTGC GTTCATGATA ACTAAAACTA AAGTCCAGGT GCTTTCAACT 
GGGGAGGTTC AGGACATTGT CAGCCGTAAA GGAGAAGATG TACAAACCTT CAACATTGAT 
AGAGGGAATA ACGAGAAAAT ACTCTCTGGG AATTTAGAAA ACATTGGATC TGAAGACCCA 
GTGGTCTGCA CTGACAAGAA ACCAGTCATC ATCATATTTG ATGAACCAAT GGATATTAAA 
TCAGCATATA AGAGGCTTTC AACAATATTT GAAGAAGGTG ATGACGATCT AGAAAAACAC 
ATGTCAGAGG CTAAAATAGA CGAAGAAGAT GAAGAAGAGG AAGCAGAAGT TCCTTCACCC 
CAAGAATCGA AAAAGGAGTT ACCAATGGAA CATACCACAA AAGCATCCTC GGAGAATCCA 
GGTTTGGAAA ACAAGTACAG ATTCAACTAT CCAACTTTAG CCGAAATGAA AAAGACCATC 
TTCGAAGACG CCAATGCCAT ACAGACCCAT TTCACAGAGG AAGACAGGTC TGAAGCTTCC 
GATGATCAGC TTGGTCAAAG GCAGGATGTC AAGAAAAAAT TCAAGTTTAA ATTTCCCAAA 
AAACAGCTCG CTGCGCTGAC TCAAGCAATC CGGACAGGGA CAAAAACTGG TAAGAAAACA 
TTACAGGTTG TGGTCTACGA AGAGGAAGAA GAAGCAGATG GGACTATAAA ACAACACAAA 
GAAGCAAAGA GATTTGAAAT TGCAAGATCA AGTAGCAAAG AGGACAATGC CAAATACGGC 
GCAGAAGCAG TGGGACTTGA GACGGACATT CAGGCGTCAC GGACAGATGA GATTAAAAGA 
ACCACCTACA AGATTCTAGA CAGTCTTGAG CAAACCATAA AGCAGCAGGA AAACCTGATG 
AGCGAAATGG CACCCATAAT AGGGGCAGAG GCTTTAGAAT CTAGAGAGAA ATGTAAAGTC 
TCCAGTGACA TCAAGACTGG TGCAGCGGAA CCAGTGGAAG AACCAAAACC TGTTGTAGAG 
ACTCCTTCCT CCATCCCTAC TGCAGCACGT AAGGGTTCCA GTGGAGGAGC ACAAACCAGC 
AGAATGCCAA TCCCAATGTC TTCTAAAAGT AGGCAAGCAA ACGTGGACAA AAGTAGCAAA 
CAGCCCAAAC TGCAGGAGCC GCAGCGCCAA TACAGACAGG CTAACGGAGG TACTAAGAAA 
GCTGGTGGGG ATGTTAAGGC TTCTGCCCCT TCTTTATCTG TTTCTAAGAT TCCAGGCCTT 
TCCTCTAACA CTGGGAAAAG TGGCTCTGTG CCTGCTCAGT CTAGCGAGAC CTCTAACCCC 
CCTAATCCCT CTGTCAAGTC ACATGTTGCC AATTCTAACC CCCAAGCTAG CCGCACCGCT 
AACCCTTCAG CCCTTGTACG TCCTGCTCAC AATGGCTCAT CCAAACTTCA GCCCCCGCCT 
ACATATAAAG GTCACCATCT TTCATTCTCA CCGCAGGCCT CAAATGGCAG ACCAGCTCTT 
CCCTCTACTA CTGCTTCTAG TTCTTCTCCC CCTTCTTCTG TCTCTCCCAC TTCACTGACT 
CAAGGTGTGA AGAGCATCCG CACAATCCAT ACACCTAGCT TCACCAGCTA CAAGTCACAG 
AACGGAAGTG CAAGCAAAGG CCTGAATGCC AAGGAGACCT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915438.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915495.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.

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CloneID	OXa12042
Clone ID Related Accession (Same CDS sequence)	XM_004915438.4 , XM_004915438.3
Accession Version	XM_004915438.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGACAAAA 
AGTCCGAAGC TGTCTTCAAG CCCACAGCCG AGCGTAGGAG ACCCGGCAGA ACATCTGTCA 
GAGGCGTCTG CTGATTCCGG AGACATGATG TTCGAAGCGG ATTCGGCAGT GCCGTTTGTC 
CGAGGCAGTC GCACAAGAGC CAGCCTTCCG GTGGTCAGAT CCACAAATCA AACGAAAGAG 
AGATCACTTG GTACGCTCTT CCTACAGTAT GGCGACGATA CCAAGAAAAT GCAGATGCCC 
AATGAAATCA CAAGCGTAGA CACGATCCGT GCCCTGTTCG TAAGTGCCTT CCCCCAGCAA 
CTGACAATGA AGATCCTGGA ATCGCCCAGC ACAGCCATTT ATATTAAGGA TGAGGGCCGA 
AATGTCTATT ATGAGCTCAG CGATGTCAGG AACATACAAG ACAGATGCTT CCTCAAAGTC 
TACAATAAAG ACCCTGCGCA TGCATTTAAC CACACTCGTG GGGCGGTCAA TGGAGATGTA 
AGGATGCAAC GAGATATTTC CTACTCGGTT CGGGAAGGCC CGGCGATCCA TAGGCCTGGC 
TCTGCTACAT ACCCCTCCCA TGCTGGCCCA GTGTCACCCC CTCCCACACC TGTTCCCCAC 
TCCATGCCAT CATCTCCATC AAGAATCCCC TATGGCGGTG GGAGGCCTGC AGTGGTACAG 
AGCAACACCA TGCAGAGGGA AAGAATACCA AGCGTGCCGG CACCTAGATC CATATCTCCT 
AGCCCCAGTG CTATTTTGGA AAGGAGAGAC GTAAAACCAG ATGAGGACAT GGGCAATAAA 
AATGTCCAGC TGCTAAGGAA TGACAGCATT TACCAAGACC CTTATTTATA CCATGAAGGA 
CGGATGAGTA TGGCATCATC ACATGGTGGG CACCCTCTCG ACGTCCCTGA TCATATTATT 
GCATACCACA GAGGGTCCTT GAGATCTGCA AGCACTTATT CTAATTCTTC TATGCAAATG 
GAGATGATGG AACAATCCAT GTACAGACAA AAAAGCCGTA AGCACGTAGA GAGTCACTTA 
CCGACACTTG GTTCTAAAAC TCCCCCAACC TCTCCTCACA GAGTGGCTGA TATAAGGATG 
ATGGAGATGC ATGCCCATAA TGCCCATCTG CCTCCCCACG GCATGCAGGC CGACAGATCT 
TCTCCTCTCC GTCAGTCATT TAAAAAGGAG CAAGGGCCTG GCATGTTTGT AGAAGCTAAA 
GTGCGAAACA CAGGTGGAAT AATGGGCATG GCGGACATTG TCCCATCTCC TACCGATAAG 
CAAGTATTTG GAGGGTATGG AGTAGCTGTG CCACCAAAAG ACCCCTATAC AAGAGAGAGG 
ATGCATGCCA TGGAGAAACA GATTGCCAGT TTAACTGGTC TTGTTCAGAC TGCACTTTTG 
AAAGGGCCCA GCCCAAACAA CTCTGCACCT AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915495.1
CDS	418..5877
Translation	MQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915438.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915438.3

ORF Insert Sequence:

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGACAAAA 
AGTCCGAAGC TGTCTTCAAG CCCACAGCCG AGCGTAGGAG ACCCGGCAGA ACATCTGTCA 
GAGGCGTCTG CTGATTCCGG AGACATGATG TTCGAAGCGG ATTCGGCAGT GCCGTTTGTC 
CGAGGCAGTC GCACAAGAGC CAGCCTTCCG GTGGTCAGAT CCACAAATCA AACGAAAGAG 
AGATCACTTG GTACGCTCTT CCTACAGTAT GGCGACGATA CCAAGAAAAT GCAGATGCCC 
AATGAAATCA CAAGCGTAGA CACGATCCGT GCCCTGTTCG TAAGTGCCTT CCCCCAGCAA 
CTGACAATGA AGATCCTGGA ATCGCCCAGC ACAGCCATTT ATATTAAGGA TGAGGGCCGA 
AATGTCTATT ATGAGCTCAG CGATGTCAGG AACATACAAG ACAGATGCTT CCTCAAAGTC 
TACAATAAAG ACCCTGCGCA TGCATTTAAC CACACTCGTG GGGCGGTCAA TGGAGATGTA 
AGGATGCAAC GAGATATTTC CTACTCGGTT CGGGAAGGCC CGGCGATCCA TAGGCCTGGC 
TCTGCTACAT ACCCCTCCCA TGCTGGCCCA GTGTCACCCC CTCCCACACC TGTTCCCCAC 
TCCATGCCAT CATCTCCATC AAGAATCCCC TATGGCGGTG GGAGGCCTGC AGTGGTACAG 
AGCAACACCA TGCAGAGGGA AAGAATACCA AGCGTGCCGG CACCTAGATC CATATCTCCT 
AGCCCCAGTG CTATTTTGGA AAGGAGAGAC GTAAAACCAG ATGAGGACAT GGGCAATAAA 
AATGTCCAGC TGCTAAGGAA TGACAGCATT TACCAAGACC CTTATTTATA CCATGAAGGA 
CGGATGAGTA TGGCATCATC ACATGGTGGG CACCCTCTCG ACGTCCCTGA TCATATTATT 
GCATACCACA GAGGGTCCTT GAGATCTGCA AGCACTTATT CTAATTCTTC TATGCAAATG 
GAGATGATGG AACAATCCAT GTACAGACAA AAAAGCCGTA AGCACGTAGA GAGTCACTTA 
CCGACACTTG GTTCTAAAAC TCCCCCAACC TCTCCTCACA GAGTGGCTGA TATAAGGATG 
ATGGAGATGC ATGCCCATAA TGCCCATCTG CCTCCCCACG GCATGCAGGC CGACAGATCT 
TCTCCTCTCC GTCAGTCATT TAAAAAGGAG CAAGGGCCTG GCATGTTTGT AGAAGCTAAA 
GTGCGAAACA CAGGTGGAAT AATGGGCATG GCGGACATTG TCCCATCTCC TACCGATAAG 
CAAGTATTTG GAGGGTATGG AGTAGCTGTG CCACCAAAAG ACCCCTATAC AAGAGAGAGG 
ATGCATGCCA TGGAGAAACA GATTGCCAGT TTAACTGGTC TTGTTCAGAC TGCACTTTTG 
AAAGGGCCCA GCCCAAACAA CTCTGCACCT AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915439.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915496.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.

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CloneID	OXa12043
Clone ID Related Accession (Same CDS sequence)	XM_004915439.3 , XM_004915439.4
Accession Version	XM_004915439.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAC AAGCTGCCCC TAAACTCCAA GAGGAGAAGG CTGAAAAGGG ATTTGTGTCA 
CTGTGCTGTG CAGCCCCCAG GCATCCATCA AGAATAAGCA AGACAAAAAG TCCGAAGCTG 
TCTTCAAGCC CACAGCCGAG CGTAGGAGAC CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT 
GATTCCGGAG ACATGATGTT CGAAGCGGAT TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC 
ACAAGAGCCA GCCTTCCGGT GGTCAGATCC ACAAATCAAA CGAAAGAGAG ATCACTTGGT 
ACGCTCTTCC TACAGTATGG CGACGATACC AAGAAAATGC AGATGCCCAA TGAAATCACA 
AGCGTAGACA CGATCCGTGC CCTGTTCGTA AGTGCCTTCC CCCAGCAACT GACAATGAAG 
ATCCTGGAAT CGCCCAGCAC AGCCATTTAT ATTAAGGATG AGGGCCGAAA TGTCTATTAT 
GAGCTCAGCG ATGTCAGGAA CATACAAGAC AGATGCTTCC TCAAAGTCTA CAATAAAGAC 
CCTGCGCATG CATTTAACCA CACTCGTGGG GCGGTCAATG GAGATGTAAG GATGCAACGA 
GATATTTCCT ACTCGGTTCG GGAAGGCCCG GCGATCCATA GGCCTGGCTC TGCTACATAC 
CCCTCCCATG CTGGCCCAGT GTCACCCCCT CCCACACCTG TTCCCCACTC CATGCCATCA 
TCTCCATCAA GAATCCCCTA TGGCGGTGGG AGGCCTGCAG TGGTACAGAG CAACACCATG 
CAGAGGGAAA GAATACCAAG CGTGCCGGCA CCTAGATCCA TATCTCCTAG CCCCAGTGCT 
ATTTTGGAAA GGAGAGACGT AAAACCAGAT GAGGACATGG GCAATAAAAA TGTCCAGCTG 
CTAAGGAATG ACAGCATTTA CCAAGACCCT TATTTATACC ATGAAGGACG GATGAGTATG 
GCATCATCAC ATGGTGGGCA CCCTCTCGAC GTCCCTGATC ATATTATTGC ATACCACAGA 
GGGTCCTTGA GATCTGCAAG CACTTATTCT AATTCTTCTA TGCAAATGGA GATGATGGAA 
CAATCCATGT ACAGACAAAA AAGCCGTAAG CACGTAGAGA GTCACTTACC GACACTTGGT 
TCTAAAACTC CCCCAACCTC TCCTCACAGA GTGGCTGATA TAAGGATGAT GGAGATGCAT 
GCCCATAATG CCCATCTGCC TCCCCACGGC ATGCAGGCCG ACAGATCTTC TCCTCTCCGT 
CAGTCATTTA AAAAGGAGCA AGGGCCTGGC ATGTTTGTAG AAGCTAAAGT GCGAAACACA 
GGTGGAATAA TGGGCATGGC GGACATTGTC CCATCTCCTA CCGATAAGCA AGTATTTGGA 
GGGTATGGAG TAGCTGTGCC ACCAAAAGAC CCCTATACAA GAGAGAGGAT GCATGCCATG 
GAGAAACAGA TTGCCAGTTT AACTGGTCTT GTTCAGACTG CACTTTTGAA AGGGCCCAGC 
CCAAACAACT CTGCACCTAG CGACAGAGTC TTAAAATCAG CCAACGGCAG CGCTTCAAGC 
TCAGATCGGC CAGGAAATCA AGGTGTTGCT GCTGCAAAGA ATTCATTGGC CATTTCAGAA 
ACAATAACTG TTATCAACCA GCAGTCGTCT ACCTGCTCGC AGGAAATGCA CGTCAGCCTA 
CAGGACATGA GGCGCAATGT GGCCGAACTT CGTCTTCAGC TGCATAAGAT GAGACAACTG 
CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG ATGATGAAGA AAGCGGAGAT GGAAATCAGC 
AGCAAAGTGA CTGAGATGGT CAAGGGCCTG GAGGACCCGG TGCAGAGACA GCGCGTCTTG 
GTGGAACAAG AGCGCCAGAA GTACCTCGGT GACGAGGAAC AAATTGTTGC AAAGTTGTGT 
GACCTTGAAG ACTTTGTGGA ATCCCTTAAA AAGGATCAGA GCACATCCCA GAGGTCGATC 
ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC ATGCTGCGCC AGATAGGAGA GGCTGTTTCC 
AACCTGAAAG GAGAGTTTCC GTACCTCCAG AATAAGATGC GGGCCGTCTT GCGTGTGGAG 
GTGGAGGCCG TCAGATTTCT GAAAGAGGAG CCCCACAAAC TGGACAGCTT ACTGAAAAGG 
ATCCGAAACA TGACAGATGC CCTTTCTGCC TTAAGAAGAC ACGCCACTGA CGGATCCTCC 
AAATCCATTG ACAACAATCA GACCAGCCAG GTTCTAATAA CAGAAAGAGT AACTGAGACT 
GATTCACTCC AGGTCCATGA GGAAAAGTCC CAAGTGTACC TTCAGCCAAC ACAAACTGAT 
GGAGGAGCAG CAGTGGAATC GCCGACCACA TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG 
GCCGGCCTCA GAGTCCATCA AGTTCAAAGT TCTCCGGTTC TCATGCAGCA GTCCCAGCAC 
TCGTCTGCAC TCGTGAACCA CACTCCTGCC CCCAGTACCC AGAGCAGTCA CAGCACAGAA 
TTACTAGTGA GCCATCAAAC TGCCAGCGTG CCACCCAGCG TCACCCCAGA AAGTGCAAGG 
AGGAACCCAC CGACGGCCGC ACAGGAAGGA TCTTCCAAGC AAAGTCTTTT TATTGAGGAA 
ATGAGTCAAG TGACTTACAG GAACCGAGCG GTGTCCATTG AGGAGGCAGA GAGAAAATTT 
GAAGAGAAGA GACAGAATCT TGATCATTAT AACGGAAAGG AATTTGAGAA AATGCTGGAA 
GAAGCACAAG CCAGTATCAT GAAGGCCATT CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG 
AAAGTGGAGA TTGTGGACAA AAAAGAGGTC ACGGAGGTAA AAACCATAAT GGAGCAGGAT 
AACGATAAAG TAACAAAGTC TCCCCCTCCA CCACCACCTC GTCGTATGTA CCCACCAGGA 
TCTGGACTGA ACAGTGTATG GACAGCAGAA TCAACGTTCT TGGTCAGGAA GGACAGCAGC 
AAGGATTCGG AGGAAGCCCT TCCTAAGGTG CCATCAAAGG CAGAAACTGA AGAGCCTCAT 
CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT TCTGCCGTAA AGGATGAGGA TGAAGAAGAG 
GAAGGAGCAA GGATTATGGC TGAACTTCAG GCCTTCCAGA AGTGTTCTTT TGTGGATGTA 
AATCCTAAAA GCCCAAATGA GCTTTCTAAA GCAGATAGCC AAGATTACAG AGCTACAGCT 
GTAACCCAAG CTAAAGAGAA GAAGGTTTCA AAAGAGGGTT TAAATGCTGA AAAGCCAGAA 
GAAAAGTGGA AAATACAAAA TCCAGCATCT GAATCTGCAA AAATTAGGCA GGGAGATACT 
GTAATTGTGA ATGAGACCTT CAGTCTGTTT ACAGGAAGGG AGAAATATGG TGAGTGCCAA 
ACTGATAAAG AGATCTTACC TGCCCAGGAA TGTGAGAAAG GAGATGGGTC CCCACTTGTG 
AGCCCAGCAA TCCTTGAGAA GAGACTCATC AGTTTTAGCA CATCAAAAGA AACAGAGAGT 
GGGATCCCTC AACCCAAAAG AGAAGTGTTA AATGTCAAAG TTGGGGAAAT TTTGCATGTA 
GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC ATGACAGCTT TAAAGTCATT AAATGAGCAT 
GAAATGCCTT ATATGGACAT GGGCCAAACT GTTGTATTGA GACCTAAGTC ATCGAGAAGA 
AGTCTTAGTC AACAACAGTC TGATGACTCC AATTCTCCAA CTACGTCACC GACTGAAACA 
ACATCTCCAG CAGATAATAT TGCGTTCATG ATAACTAAAA CTAAAGTCCA GGTGCTTTCA 
ACTGGGGAGG TTCAGGACAT TGTCAGCCGT AAAGGAGAAG ATGTACAAAC CTTCAACATT 
GATAGAGGGA ATAACGAGAA AATACTCTCT GGGAATTTAG AAAACATTGG ATCTGAAGAC 
CCAGTGGTCT GCACTGACAA GAAACCAGTC ATCATCATAT TTGATGAACC AATGGATATT 
AAATCAGCAT ATAAGAGGCT TTCAACAATA TTTGAAGAAG GTGATGACGA TCTAGAAAAA 
CACATGTCAG AGGCTAAAAT AGACGAAGAA GATGAAGAAG AGGAAGCAGA AGTTCCTTCA 
CCCCAAGAAT CGAAAAAGGA GTTACCAATG GAACATACCA CAAAAGCATC CTCGGAGAAT 
CCAGGTTTGG AAAACAAGTA CAGATTCAAC TATCCAACTT TAGCCGAAAT GAAAAAGACC 
ATCTTCGAAG ACGCCAATGC CATACAGACC CATTTCACAG AGGAAGACAG GTCTGAAGCT 
TCCGATGATC AGCTTGGTCA AAGGCAGGAT GTCAAGAAAA AATTCAAGTT TAAATTTCCC 
AAAAAACAGC TCGCTGCGCT GACTCAAGCA ATCCGGACAG GGACAAAAAC TGGTAAGAAA 
ACATTACAGG TTGTGGTCTA CGAAGAGGAA GAAGAAGCAG ATGGGACTAT AAAACAACAC 
AAAGAAGCAA AGAGATTTGA AATTGCAAGA TCAAGTAGCA AAGAGGACAA TGCCAAATAC 
GGCGCAGAAG CAGTGGGACT TGAGACGGAC ATTCAGGCGT CACGGACAGA TGAGATTAAA 
AGAACCACCT ACAAGATTCT AGACAGTCTT GAGCAAACCA TAAAGCAGCA GGAAAACCTG 
ATGAGCGAAA TGGCACCCAT AATAGGGGCA GAGGCTTTAG AATCTAGAGA GAAATGTAAA 
GTCTCCAGTG ACATCAAGAC TGGTGCAGCG GAACCAGTGG AAGAACCAAA ACCTGTTGTA 
GAGACTCCTT CCTCCATCCC TACTGCAGCA CGTAAGGGTT CCAGTGGAGG AGCACAAACC 
AGCAGAATGC CAATCCCAAT GTCTTCTAAA AGTAGGCAAG CAAACGTGGA CAAAAGTAGC 
AAACAGCCCA AACTGCAGGA GCCGCAGCGC CAATACAGAC AGGCTAACGG AGGTACTAAG 
AAAGCTGGTG GGGATGTTAA GGCTTCTGCC CCTTCTTTAT CTGTTTCTAA GATTCCAGGC 
CTTTCCTCTA ACACTGGGAA AAGTGGCTCT GTGCCTGCTC AGTCTAGCGA GACCTCTAAC 
CCCCCTAATC CCTCTGTCAA GTCACATGTT GCCAATTCTA ACCCCCAAGC TAGCCGCACC 
GCTAACCCTT CAGCCCTTGT ACGTCCTGCT CACAATGGCT CATCCAAACT TCAGCCCCCG 
CCTACATATA AAGGTCACCA TCTTTCATTC TCACCGCAGG CCTCAAATGG CAGACCAGCT 
CTTCCCTCTA CTACTGCTTC TAGTTCTTCT CCCCCTTCTT CTGTCTCTCC CACTTCACTG 
ACTCAAGGTG TGAAGAGCAT CCGCACAATC CATACACCTA GCTTCACCAG CTACAAGTCA 
CAGAACGGAA GTGCAAGCAA AGGCCTGAAT GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915496.1
CDS	128..5575
Translation	MEQQAAPKLQEEKAEKGFVSLCCAAPRHPSRISKTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915439.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915439.3

ORF Insert Sequence:

ATGGAACAAC AAGCTGCCCC TAAACTCCAA GAGGAGAAGG CTGAAAAGGG ATTTGTGTCA 
CTGTGCTGTG CAGCCCCCAG GCATCCATCA AGAATAAGCA AGACAAAAAG TCCGAAGCTG 
TCTTCAAGCC CACAGCCGAG CGTAGGAGAC CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT 
GATTCCGGAG ACATGATGTT CGAAGCGGAT TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC 
ACAAGAGCCA GCCTTCCGGT GGTCAGATCC ACAAATCAAA CGAAAGAGAG ATCACTTGGT 
ACGCTCTTCC TACAGTATGG CGACGATACC AAGAAAATGC AGATGCCCAA TGAAATCACA 
AGCGTAGACA CGATCCGTGC CCTGTTCGTA AGTGCCTTCC CCCAGCAACT GACAATGAAG 
ATCCTGGAAT CGCCCAGCAC AGCCATTTAT ATTAAGGATG AGGGCCGAAA TGTCTATTAT 
GAGCTCAGCG ATGTCAGGAA CATACAAGAC AGATGCTTCC TCAAAGTCTA CAATAAAGAC 
CCTGCGCATG CATTTAACCA CACTCGTGGG GCGGTCAATG GAGATGTAAG GATGCAACGA 
GATATTTCCT ACTCGGTTCG GGAAGGCCCG GCGATCCATA GGCCTGGCTC TGCTACATAC 
CCCTCCCATG CTGGCCCAGT GTCACCCCCT CCCACACCTG TTCCCCACTC CATGCCATCA 
TCTCCATCAA GAATCCCCTA TGGCGGTGGG AGGCCTGCAG TGGTACAGAG CAACACCATG 
CAGAGGGAAA GAATACCAAG CGTGCCGGCA CCTAGATCCA TATCTCCTAG CCCCAGTGCT 
ATTTTGGAAA GGAGAGACGT AAAACCAGAT GAGGACATGG GCAATAAAAA TGTCCAGCTG 
CTAAGGAATG ACAGCATTTA CCAAGACCCT TATTTATACC ATGAAGGACG GATGAGTATG 
GCATCATCAC ATGGTGGGCA CCCTCTCGAC GTCCCTGATC ATATTATTGC ATACCACAGA 
GGGTCCTTGA GATCTGCAAG CACTTATTCT AATTCTTCTA TGCAAATGGA GATGATGGAA 
CAATCCATGT ACAGACAAAA AAGCCGTAAG CACGTAGAGA GTCACTTACC GACACTTGGT 
TCTAAAACTC CCCCAACCTC TCCTCACAGA GTGGCTGATA TAAGGATGAT GGAGATGCAT 
GCCCATAATG CCCATCTGCC TCCCCACGGC ATGCAGGCCG ACAGATCTTC TCCTCTCCGT 
CAGTCATTTA AAAAGGAGCA AGGGCCTGGC ATGTTTGTAG AAGCTAAAGT GCGAAACACA 
GGTGGAATAA TGGGCATGGC GGACATTGTC CCATCTCCTA CCGATAAGCA AGTATTTGGA 
GGGTATGGAG TAGCTGTGCC ACCAAAAGAC CCCTATACAA GAGAGAGGAT GCATGCCATG 
GAGAAACAGA TTGCCAGTTT AACTGGTCTT GTTCAGACTG CACTTTTGAA AGGGCCCAGC 
CCAAACAACT CTGCACCTAG CGACAGAGTC TTAAAATCAG CCAACGGCAG CGCTTCAAGC 
TCAGATCGGC CAGGAAATCA AGGTGTTGCT GCTGCAAAGA ATTCATTGGC CATTTCAGAA 
ACAATAACTG TTATCAACCA GCAGTCGTCT ACCTGCTCGC AGGAAATGCA CGTCAGCCTA 
CAGGACATGA GGCGCAATGT GGCCGAACTT CGTCTTCAGC TGCATAAGAT GAGACAACTG 
CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG ATGATGAAGA AAGCGGAGAT GGAAATCAGC 
AGCAAAGTGA CTGAGATGGT CAAGGGCCTG GAGGACCCGG TGCAGAGACA GCGCGTCTTG 
GTGGAACAAG AGCGCCAGAA GTACCTCGGT GACGAGGAAC AAATTGTTGC AAAGTTGTGT 
GACCTTGAAG ACTTTGTGGA ATCCCTTAAA AAGGATCAGA GCACATCCCA GAGGTCGATC 
ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC ATGCTGCGCC AGATAGGAGA GGCTGTTTCC 
AACCTGAAAG GAGAGTTTCC GTACCTCCAG AATAAGATGC GGGCCGTCTT GCGTGTGGAG 
GTGGAGGCCG TCAGATTTCT GAAAGAGGAG CCCCACAAAC TGGACAGCTT ACTGAAAAGG 
ATCCGAAACA TGACAGATGC CCTTTCTGCC TTAAGAAGAC ACGCCACTGA CGGATCCTCC 
AAATCCATTG ACAACAATCA GACCAGCCAG GTTCTAATAA CAGAAAGAGT AACTGAGACT 
GATTCACTCC AGGTCCATGA GGAAAAGTCC CAAGTGTACC TTCAGCCAAC ACAAACTGAT 
GGAGGAGCAG CAGTGGAATC GCCGACCACA TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG 
GCCGGCCTCA GAGTCCATCA AGTTCAAAGT TCTCCGGTTC TCATGCAGCA GTCCCAGCAC 
TCGTCTGCAC TCGTGAACCA CACTCCTGCC CCCAGTACCC AGAGCAGTCA CAGCACAGAA 
TTACTAGTGA GCCATCAAAC TGCCAGCGTG CCACCCAGCG TCACCCCAGA AAGTGCAAGG 
AGGAACCCAC CGACGGCCGC ACAGGAAGGA TCTTCCAAGC AAAGTCTTTT TATTGAGGAA 
ATGAGTCAAG TGACTTACAG GAACCGAGCG GTGTCCATTG AGGAGGCAGA GAGAAAATTT 
GAAGAGAAGA GACAGAATCT TGATCATTAT AACGGAAAGG AATTTGAGAA AATGCTGGAA 
GAAGCACAAG CCAGTATCAT GAAGGCCATT CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG 
AAAGTGGAGA TTGTGGACAA AAAAGAGGTC ACGGAGGTAA AAACCATAAT GGAGCAGGAT 
AACGATAAAG TAACAAAGTC TCCCCCTCCA CCACCACCTC GTCGTATGTA CCCACCAGGA 
TCTGGACTGA ACAGTGTATG GACAGCAGAA TCAACGTTCT TGGTCAGGAA GGACAGCAGC 
AAGGATTCGG AGGAAGCCCT TCCTAAGGTG CCATCAAAGG CAGAAACTGA AGAGCCTCAT 
CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT TCTGCCGTAA AGGATGAGGA TGAAGAAGAG 
GAAGGAGCAA GGATTATGGC TGAACTTCAG GCCTTCCAGA AGTGTTCTTT TGTGGATGTA 
AATCCTAAAA GCCCAAATGA GCTTTCTAAA GCAGATAGCC AAGATTACAG AGCTACAGCT 
GTAACCCAAG CTAAAGAGAA GAAGGTTTCA AAAGAGGGTT TAAATGCTGA AAAGCCAGAA 
GAAAAGTGGA AAATACAAAA TCCAGCATCT GAATCTGCAA AAATTAGGCA GGGAGATACT 
GTAATTGTGA ATGAGACCTT CAGTCTGTTT ACAGGAAGGG AGAAATATGG TGAGTGCCAA 
ACTGATAAAG AGATCTTACC TGCCCAGGAA TGTGAGAAAG GAGATGGGTC CCCACTTGTG 
AGCCCAGCAA TCCTTGAGAA GAGACTCATC AGTTTTAGCA CATCAAAAGA AACAGAGAGT 
GGGATCCCTC AACCCAAAAG AGAAGTGTTA AATGTCAAAG TTGGGGAAAT TTTGCATGTA 
GAAAAGAAGG AAGAGGTGCT AGAAAGTCAC ATGACAGCTT TAAAGTCATT AAATGAGCAT 
GAAATGCCTT ATATGGACAT GGGCCAAACT GTTGTATTGA GACCTAAGTC ATCGAGAAGA 
AGTCTTAGTC AACAACAGTC TGATGACTCC AATTCTCCAA CTACGTCACC GACTGAAACA 
ACATCTCCAG CAGATAATAT TGCGTTCATG ATAACTAAAA CTAAAGTCCA GGTGCTTTCA 
ACTGGGGAGG TTCAGGACAT TGTCAGCCGT AAAGGAGAAG ATGTACAAAC CTTCAACATT 
GATAGAGGGA ATAACGAGAA AATACTCTCT GGGAATTTAG AAAACATTGG ATCTGAAGAC 
CCAGTGGTCT GCACTGACAA GAAACCAGTC ATCATCATAT TTGATGAACC AATGGATATT 
AAATCAGCAT ATAAGAGGCT TTCAACAATA TTTGAAGAAG GTGATGACGA TCTAGAAAAA 
CACATGTCAG AGGCTAAAAT AGACGAAGAA GATGAAGAAG AGGAAGCAGA AGTTCCTTCA 
CCCCAAGAAT CGAAAAAGGA GTTACCAATG GAACATACCA CAAAAGCATC CTCGGAGAAT 
CCAGGTTTGG AAAACAAGTA CAGATTCAAC TATCCAACTT TAGCCGAAAT GAAAAAGACC 
ATCTTCGAAG ACGCCAATGC CATACAGACC CATTTCACAG AGGAAGACAG GTCTGAAGCT 
TCCGATGATC AGCTTGGTCA AAGGCAGGAT GTCAAGAAAA AATTCAAGTT TAAATTTCCC 
AAAAAACAGC TCGCTGCGCT GACTCAAGCA ATCCGGACAG GGACAAAAAC TGGTAAGAAA 
ACATTACAGG TTGTGGTCTA CGAAGAGGAA GAAGAAGCAG ATGGGACTAT AAAACAACAC 
AAAGAAGCAA AGAGATTTGA AATTGCAAGA TCAAGTAGCA AAGAGGACAA TGCCAAATAC 
GGCGCAGAAG CAGTGGGACT TGAGACGGAC ATTCAGGCGT CACGGACAGA TGAGATTAAA 
AGAACCACCT ACAAGATTCT AGACAGTCTT GAGCAAACCA TAAAGCAGCA GGAAAACCTG 
ATGAGCGAAA TGGCACCCAT AATAGGGGCA GAGGCTTTAG AATCTAGAGA GAAATGTAAA 
GTCTCCAGTG ACATCAAGAC TGGTGCAGCG GAACCAGTGG AAGAACCAAA ACCTGTTGTA 
GAGACTCCTT CCTCCATCCC TACTGCAGCA CGTAAGGGTT CCAGTGGAGG AGCACAAACC 
AGCAGAATGC CAATCCCAAT GTCTTCTAAA AGTAGGCAAG CAAACGTGGA CAAAAGTAGC 
AAACAGCCCA AACTGCAGGA GCCGCAGCGC CAATACAGAC AGGCTAACGG AGGTACTAAG 
AAAGCTGGTG GGGATGTTAA GGCTTCTGCC CCTTCTTTAT CTGTTTCTAA GATTCCAGGC 
CTTTCCTCTA ACACTGGGAA AAGTGGCTCT GTGCCTGCTC AGTCTAGCGA GACCTCTAAC 
CCCCCTAATC CCTCTGTCAA GTCACATGTT GCCAATTCTA ACCCCCAAGC TAGCCGCACC 
GCTAACCCTT CAGCCCTTGT ACGTCCTGCT CACAATGGCT CATCCAAACT TCAGCCCCCG 
CCTACATATA AAGGTCACCA TCTTTCATTC TCACCGCAGG CCTCAAATGG CAGACCAGCT 
CTTCCCTCTA CTACTGCTTC TAGTTCTTCT CCCCCTTCTT CTGTCTCTCC CACTTCACTG 
ACTCAAGGTG TGAAGAGCAT CCGCACAATC CATACACCTA GCTTCACCAG CTACAAGTCA 
CAGAACGGAA GTGCAAGCAA AGGCCTGAAT GCCAAGGAGA CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_018094778.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950267.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.

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CloneID	OXa12044
Clone ID Related Accession (Same CDS sequence)	XM_018094778.1 , XM_004915440.3 , XM_004915440.4 , XM_018094778.2
Accession Version	XM_018094778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950267.1
CDS	109..5364
Translation	MMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_018094778.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094778.1

ORF Insert Sequence:

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915440.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915497.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.

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CloneID	OXa12044
Clone ID Related Accession (Same CDS sequence)	XM_018094778.1 , XM_004915440.3 , XM_004915440.4 , XM_018094778.2
Accession Version	XM_004915440.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915497.1
CDS	492..5747
Translation	MMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915440.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915440.3

ORF Insert Sequence:

ATGATGTTCG AAGCGGATTC GGCAGTGCCG TTTGTCCGAG GCAGTCGCAC AAGAGCCAGC 
CTTCCGGTGG TCAGATCCAC AAATCAAACG AAAGAGAGAT CACTTGGTAC GCTCTTCCTA 
CAGTATGGCG ACGATACCAA GAAAATGCAG ATGCCCAATG AAATCACAAG CGTAGACACG 
ATCCGTGCCC TGTTCGTAAG TGCCTTCCCC CAGCAACTGA CAATGAAGAT CCTGGAATCG 
CCCAGCACAG CCATTTATAT TAAGGATGAG GGCCGAAATG TCTATTATGA GCTCAGCGAT 
GTCAGGAACA TACAAGACAG ATGCTTCCTC AAAGTCTACA ATAAAGACCC TGCGCATGCA 
TTTAACCACA CTCGTGGGGC GGTCAATGGA GATGTAAGGA TGCAACGAGA TATTTCCTAC 
TCGGTTCGGG AAGGCCCGGC GATCCATAGG CCTGGCTCTG CTACATACCC CTCCCATGCT 
GGCCCAGTGT CACCCCCTCC CACACCTGTT CCCCACTCCA TGCCATCATC TCCATCAAGA 
ATCCCCTATG GCGGTGGGAG GCCTGCAGTG GTACAGAGCA ACACCATGCA GAGGGAAAGA 
ATACCAAGCG TGCCGGCACC TAGATCCATA TCTCCTAGCC CCAGTGCTAT TTTGGAAAGG 
AGAGACGTAA AACCAGATGA GGACATGGGC AATAAAAATG TCCAGCTGCT AAGGAATGAC 
AGCATTTACC AAGACCCTTA TTTATACCAT GAAGGACGGA TGAGTATGGC ATCATCACAT 
GGTGGGCACC CTCTCGACGT CCCTGATCAT ATTATTGCAT ACCACAGAGG GTCCTTGAGA 
TCTGCAAGCA CTTATTCTAA TTCTTCTATG CAAATGGAGA TGATGGAACA ATCCATGTAC 
AGACAAAAAA GCCGTAAGCA CGTAGAGAGT CACTTACCGA CACTTGGTTC TAAAACTCCC 
CCAACCTCTC CTCACAGAGT GGCTGATATA AGGATGATGG AGATGCATGC CCATAATGCC 
CATCTGCCTC CCCACGGCAT GCAGGCCGAC AGATCTTCTC CTCTCCGTCA GTCATTTAAA 
AAGGAGCAAG GGCCTGGCAT GTTTGTAGAA GCTAAAGTGC GAAACACAGG TGGAATAATG 
GGCATGGCGG ACATTGTCCC ATCTCCTACC GATAAGCAAG TATTTGGAGG GTATGGAGTA 
GCTGTGCCAC CAAAAGACCC CTATACAAGA GAGAGGATGC ATGCCATGGA GAAACAGATT 
GCCAGTTTAA CTGGTCTTGT TCAGACTGCA CTTTTGAAAG GGCCCAGCCC AAACAACTCT 
GCACCTAGCG ACAGAGTCTT AAAATCAGCC AACGGCAGCG CTTCAAGCTC AGATCGGCCA 
GGAAATCAAG GTGTTGCTGC TGCAAAGAAT TCATTGGCCA TTTCAGAAAC AATAACTGTT 
ATCAACCAGC AGTCGTCTAC CTGCTCGCAG GAAATGCACG TCAGCCTACA GGACATGAGG 
CGCAATGTGG CCGAACTTCG TCTTCAGCTG CATAAGATGA GACAACTGCA GCTGCAGAAC 
CAGGAGACGG TGAGGCTGAT GATGAAGAAA GCGGAGATGG AAATCAGCAG CAAAGTGACT 
GAGATGGTCA AGGGCCTGGA GGACCCGGTG CAGAGACAGC GCGTCTTGGT GGAACAAGAG 
CGCCAGAAGT ACCTCGGTGA CGAGGAACAA ATTGTTGCAA AGTTGTGTGA CCTTGAAGAC 
TTTGTGGAAT CCCTTAAAAA GGATCAGAGC ACATCCCAGA GGTCGATCAC CCTAAAGGAC 
GTTGAGGATC GGGCCTTCAT GCTGCGCCAG ATAGGAGAGG CTGTTTCCAA CCTGAAAGGA 
GAGTTTCCGT ACCTCCAGAA TAAGATGCGG GCCGTCTTGC GTGTGGAGGT GGAGGCCGTC 
AGATTTCTGA AAGAGGAGCC CCACAAACTG GACAGCTTAC TGAAAAGGAT CCGAAACATG 
ACAGATGCCC TTTCTGCCTT AAGAAGACAC GCCACTGACG GATCCTCCAA ATCCATTGAC 
AACAATCAGA CCAGCCAGGT TCTAATAACA GAAAGAGTAA CTGAGACTGA TTCACTCCAG 
GTCCATGAGG AAAAGTCCCA AGTGTACCTT CAGCCAACAC AAACTGATGG AGGAGCAGCA 
GTGGAATCGC CGACCACATC TGTTAAATCG GAAGTGGTTC CCCTGTCGGC CGGCCTCAGA 
GTCCATCAAG TTCAAAGTTC TCCGGTTCTC ATGCAGCAGT CCCAGCACTC GTCTGCACTC 
GTGAACCACA CTCCTGCCCC CAGTACCCAG AGCAGTCACA GCACAGAATT ACTAGTGAGC 
CATCAAACTG CCAGCGTGCC ACCCAGCGTC ACCCCAGAAA GTGCAAGGAG GAACCCACCG 
ACGGCCGCAC AGGAAGGATC TTCCAAGCAA AGTCTTTTTA TTGAGGAAAT GAGTCAAGTG 
ACTTACAGGA ACCGAGCGGT GTCCATTGAG GAGGCAGAGA GAAAATTTGA AGAGAAGAGA 
CAGAATCTTG ATCATTATAA CGGAAAGGAA TTTGAGAAAA TGCTGGAAGA AGCACAAGCC 
AGTATCATGA AGGCCATTCC CAGCTTAGAG GTGCCAGCCC AGTCCCAGAA AGTGGAGATT 
GTGGACAAAA AAGAGGTCAC GGAGGTAAAA ACCATAATGG AGCAGGATAA CGATAAAGTA 
ACAAAGTCTC CCCCTCCACC ACCACCTCGT CGTATGTACC CACCAGGATC TGGACTGAAC 
AGTGTATGGA CAGCAGAATC AACGTTCTTG GTCAGGAAGG ACAGCAGCAA GGATTCGGAG 
GAAGCCCTTC CTAAGGTGCC ATCAAAGGCA GAAACTGAAG AGCCTCATCT TCAGTCGGTG 
GAGGCTACAG TCAAGTCTTC TGCCGTAAAG GATGAGGATG AAGAAGAGGA AGGAGCAAGG 
ATTATGGCTG AACTTCAGGC CTTCCAGAAG TGTTCTTTTG TGGATGTAAA TCCTAAAAGC 
CCAAATGAGC TTTCTAAAGC AGATAGCCAA GATTACAGAG CTACAGCTGT AACCCAAGCT 
AAAGAGAAGA AGGTTTCAAA AGAGGGTTTA AATGCTGAAA AGCCAGAAGA AAAGTGGAAA 
ATACAAAATC CAGCATCTGA ATCTGCAAAA ATTAGGCAGG GAGATACTGT AATTGTGAAT 
GAGACCTTCA GTCTGTTTAC AGGAAGGGAG AAATATGGTG AGTGCCAAAC TGATAAAGAG 
ATCTTACCTG CCCAGGAATG TGAGAAAGGA GATGGGTCCC CACTTGTGAG CCCAGCAATC 
CTTGAGAAGA GACTCATCAG TTTTAGCACA TCAAAAGAAA CAGAGAGTGG GATCCCTCAA 
CCCAAAAGAG AAGTGTTAAA TGTCAAAGTT GGGGAAATTT TGCATGTAGA AAAGAAGGAA 
GAGGTGCTAG AAAGTCACAT GACAGCTTTA AAGTCATTAA ATGAGCATGA AATGCCTTAT 
ATGGACATGG GCCAAACTGT TGTATTGAGA CCTAAGTCAT CGAGAAGAAG TCTTAGTCAA 
CAACAGTCTG ATGACTCCAA TTCTCCAACT ACGTCACCGA CTGAAACAAC ATCTCCAGCA 
GATAATATTG CGTTCATGAT AACTAAAACT AAAGTCCAGG TGCTTTCAAC TGGGGAGGTT 
CAGGACATTG TCAGCCGTAA AGGAGAAGAT GTACAAACCT TCAACATTGA TAGAGGGAAT 
AACGAGAAAA TACTCTCTGG GAATTTAGAA AACATTGGAT CTGAAGACCC AGTGGTCTGC 
ACTGACAAGA AACCAGTCAT CATCATATTT GATGAACCAA TGGATATTAA ATCAGCATAT 
AAGAGGCTTT CAACAATATT TGAAGAAGGT GATGACGATC TAGAAAAACA CATGTCAGAG 
GCTAAAATAG ACGAAGAAGA TGAAGAAGAG GAAGCAGAAG TTCCTTCACC CCAAGAATCG 
AAAAAGGAGT TACCAATGGA ACATACCACA AAAGCATCCT CGGAGAATCC AGGTTTGGAA 
AACAAGTACA GATTCAACTA TCCAACTTTA GCCGAAATGA AAAAGACCAT CTTCGAAGAC 
GCCAATGCCA TACAGACCCA TTTCACAGAG GAAGACAGGT CTGAAGCTTC CGATGATCAG 
CTTGGTCAAA GGCAGGATGT CAAGAAAAAA TTCAAGTTTA AATTTCCCAA AAAACAGCTC 
GCTGCGCTGA CTCAAGCAAT CCGGACAGGG ACAAAAACTG GTAAGAAAAC ATTACAGGTT 
GTGGTCTACG AAGAGGAAGA AGAAGCAGAT GGGACTATAA AACAACACAA AGAAGCAAAG 
AGATTTGAAA TTGCAAGATC AAGTAGCAAA GAGGACAATG CCAAATACGG CGCAGAAGCA 
GTGGGACTTG AGACGGACAT TCAGGCGTCA CGGACAGATG AGATTAAAAG AACCACCTAC 
AAGATTCTAG ACAGTCTTGA GCAAACCATA AAGCAGCAGG AAAACCTGAT GAGCGAAATG 
GCACCCATAA TAGGGGCAGA GGCTTTAGAA TCTAGAGAGA AATGTAAAGT CTCCAGTGAC 
ATCAAGACTG GTGCAGCGGA ACCAGTGGAA GAACCAAAAC CTGTTGTAGA GACTCCTTCC 
TCCATCCCTA CTGCAGCACG TAAGGGTTCC AGTGGAGGAG CACAAACCAG CAGAATGCCA 
ATCCCAATGT CTTCTAAAAG TAGGCAAGCA AACGTGGACA AAAGTAGCAA ACAGCCCAAA 
CTGCAGGAGC CGCAGCGCCA ATACAGACAG GCTAACGGAG GTACTAAGAA AGCTGGTGGG 
GATGTTAAGG CTTCTGCCCC TTCTTTATCT GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC 
ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG TCTAGCGAGA CCTCTAACCC CCCTAATCCC 
TCTGTCAAGT CACATGTTGC CAATTCTAAC CCCCAAGCTA GCCGCACCGC TAACCCTTCA 
GCCCTTGTAC GTCCTGCTCA CAATGGCTCA TCCAAACTTC AGCCCCCGCC TACATATAAA 
GGTCACCATC TTTCATTCTC ACCGCAGGCC TCAAATGGCA GACCAGCTCT TCCCTCTACT 
ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT GTCTCTCCCA CTTCACTGAC TCAAGGTGTG 
AAGAGCATCC GCACAATCCA TACACCTAGC TTCACCAGCT ACAAGTCACA GAACGGAAGT 
GCAAGCAAAG GCCTGAATGC CAAGGAGACC TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_004915443.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915500.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.

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CloneID	OXa12045
Clone ID Related Accession (Same CDS sequence)	XM_004915443.3 , XM_004915443.4
Accession Version	XM_004915443.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAG AACTTAGGGA CCATGGAAAA GGCACTGTGA ACATACAAGA CAGATGCTTC 
CTCAAAGTCT ACAATAAAGA CCCTGCGCAT GCATTTAACC ACACTCGTGG GGCGGTCAAT 
GGAGATGTAA GGATGCAACG AGATATTTCC TACTCGGTTC GGGAAGGCCC GGCGATCCAT 
AGGCCTGGCT CTGCTACATA CCCCTCCCAT GCTGGCCCAG TGTCACCCCC TCCCACACCT 
GTTCCCCACT CCATGCCATC ATCTCCATCA AGAATCCCCT ATGGCGGTGG GAGGCCTGCA 
GTGGTACAGA GCAACACCAT GCAGAGGGAA AGAATACCAA GCGTGCCGGC ACCTAGATCC 
ATATCTCCTA GCCCCAGTGC TATTTTGGAA AGGAGAGACG TAAAACCAGA TGAGGACATG 
GGCAATAAAA ATGTCCAGCT GCTAAGGAAT GACAGCATTT ACCAAGACCC TTATTTATAC 
CATGAAGGAC GGATGAGTAT GGCATCATCA CATGGTGGGC ACCCTCTCGA CGTCCCTGAT 
CATATTATTG CATACCACAG AGGGTCCTTG AGATCTGCAA GCACTTATTC TAATTCTTCT 
ATGCAAATGG AGATGATGGA ACAATCCATG TACAGACAAA AAAGCCGTAA GCACGTAGAG 
AGTCACTTAC CGACACTTGG TTCTAAAACT CCCCCAACCT CTCCTCACAG AGTGGCTGAT 
ATAAGGATGA TGGAGATGCA TGCCCATAAT GCCCATCTGC CTCCCCACGG CATGCAGGCC 
GACAGATCTT CTCCTCTCCG TCAGTCATTT AAAAAGGAGC AAGGGCCTGG CATGTTTGTA 
GAAGCTAAAG TGCGAAACAC AGGTGGAATA ATGGGCATGG CGGACATTGT CCCATCTCCT 
ACCGATAAGC AAGTATTTGG AGGGTATGGA GTAGCTGTGC CACCAAAAGA CCCCTATACA 
AGAGAGAGGA TGCATGCCAT GGAGAAACAG ATTGCCAGTT TAACTGGTCT TGTTCAGACT 
GCACTTTTGA AAGGGCCCAG CCCAAACAAC TCTGCACCTA GCGACAGAGT CTTAAAATCA 
GCCAACGGCA GCGCTTCAAG CTCAGATCGG CCAGGAAATC AAGGTGTTGC TGCTGCAAAG 
AATTCATTGG CCATTTCAGA AACAATAACT GTTATCAACC AGCAGTCGTC TACCTGCTCG 
CAGGAAATGC ACGTCAGCCT ACAGGACATG AGGCGCAATG TGGCCGAACT TCGTCTTCAG 
CTGCATAAGA TGAGACAACT GCAGCTGCAG AACCAGGAGA CGGTGAGGCT GATGATGAAG 
AAAGCGGAGA TGGAAATCAG CAGCAAAGTG ACTGAGATGG TCAAGGGCCT GGAGGACCCG 
GTGCAGAGAC AGCGCGTCTT GGTGGAACAA GAGCGCCAGA AGTACCTCGG TGACGAGGAA 
CAAATTGTTG CAAAGTTGTG TGACCTTGAA GACTTTGTGG AATCCCTTAA AAAGGATCAG 
AGCACATCCC AGAGGTCGAT CACCCTAAAG GACGTTGAGG ATCGGGCCTT CATGCTGCGC 
CAGATAGGAG AGGCTGTTTC CAACCTGAAA GGAGAGTTTC CGTACCTCCA GAATAAGATG 
CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC GTCAGATTTC TGAAAGAGGA GCCCCACAAA 
CTGGACAGCT TACTGAAAAG GATCCGAAAC ATGACAGATG CCCTTTCTGC CTTAAGAAGA 
CACGCCACTG ACGGATCCTC CAAATCCATT GACAACAATC AGACCAGCCA GGTTCTAATA 
ACAGAAAGAG TAACTGAGAC TGATTCACTC CAGGTCCATG AGGAAAAGTC CCAAGTGTAC 
CTTCAGCCAA CACAAACTGA TGGAGGAGCA GCAGTGGAAT CGCCGACCAC ATCTGTTAAA 
TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC AGAGTCCATC AAGTTCAAAG TTCTCCGGTT 
CTCATGCAGC AGTCCCAGCA CTCGTCTGCA CTCGTGAACC ACACTCCTGC CCCCAGTACC 
CAGAGCAGTC ACAGCACAGA ATTACTAGTG AGCCATCAAA CTGCCAGCGT GCCACCCAGC 
GTCACCCCAG AAAGTGCAAG GAGGAACCCA CCGACGGCCG CACAGGAAGG ATCTTCCAAG 
CAAAGTCTTT TTATTGAGGA AATGAGTCAA GTGACTTACA GGAACCGAGC GGTGTCCATT 
GAGGAGGCAG AGAGAAAATT TGAAGAGAAG AGACAGAATC TTGATCATTA TAACGGAAAG 
GAATTTGAGA AAATGCTGGA AGAAGCACAA GCCAGTATCA TGAAGGCCAT TCCCAGCTTA 
GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG ATTGTGGACA AAAAAGAGGT CACGGAGGTA 
AAAACCATAA TGGAGCAGGA TAACGATAAA GTAACAAAGT CTCCCCCTCC ACCACCACCT 
CGTCGTATGT ACCCACCAGG ATCTGGACTG AACAGTGTAT GGACAGCAGA ATCAACGTTC 
TTGGTCAGGA AGGACAGCAG CAAGGATTCG GAGGAAGCCC TTCCTAAGGT GCCATCAAAG 
GCAGAAACTG AAGAGCCTCA TCTTCAGTCG GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA 
AAGGATGAGG ATGAAGAAGA GGAAGGAGCA AGGATTATGG CTGAACTTCA GGCCTTCCAG 
AAGTGTTCTT TTGTGGATGT AAATCCTAAA AGCCCAAATG AGCTTTCTAA AGCAGATAGC 
CAAGATTACA GAGCTACAGC TGTAACCCAA GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT 
TTAAATGCTG AAAAGCCAGA AGAAAAGTGG AAAATACAAA ATCCAGCATC TGAATCTGCA 
AAAATTAGGC AGGGAGATAC TGTAATTGTG AATGAGACCT TCAGTCTGTT TACAGGAAGG 
GAGAAATATG GTGAGTGCCA AACTGATAAA GAGATCTTAC CTGCCCAGGA ATGTGAGAAA 
GGAGATGGGT CCCCACTTGT GAGCCCAGCA ATCCTTGAGA AGAGACTCAT CAGTTTTAGC 
ACATCAAAAG AAACAGAGAG TGGGATCCCT CAACCCAAAA GAGAAGTGTT AAATGTCAAA 
GTTGGGGAAA TTTTGCATGT AGAAAAGAAG GAAGAGGTGC TAGAAAGTCA CATGACAGCT 
TTAAAGTCAT TAAATGAGCA TGAAATGCCT TATATGGACA TGGGCCAAAC TGTTGTATTG 
AGACCTAAGT CATCGAGAAG AAGTCTTAGT CAACAACAGT CTGATGACTC CAATTCTCCA 
ACTACGTCAC CGACTGAAAC AACATCTCCA GCAGATAATA TTGCGTTCAT GATAACTAAA 
ACTAAAGTCC AGGTGCTTTC AACTGGGGAG GTTCAGGACA TTGTCAGCCG TAAAGGAGAA 
GATGTACAAA CCTTCAACAT TGATAGAGGG AATAACGAGA AAATACTCTC TGGGAATTTA 
GAAAACATTG GATCTGAAGA CCCAGTGGTC TGCACTGACA AGAAACCAGT CATCATCATA 
TTTGATGAAC CAATGGATAT TAAATCAGCA TATAAGAGGC TTTCAACAAT ATTTGAAGAA 
GGTGATGACG ATCTAGAAAA ACACATGTCA GAGGCTAAAA TAGACGAAGA AGATGAAGAA 
GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA TCGAAAAAGG AGTTACCAAT GGAACATACC 
ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG GAAAACAAGT ACAGATTCAA CTATCCAACT 
TTAGCCGAAA TGAAAAAGAC CATCTTCGAA GACGCCAATG CCATACAGAC CCATTTCACA 
GAGGAAGACA GGTCTGAAGC TTCCGATGAT CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA 
AAATTCAAGT TTAAATTTCC CAAAAAACAG CTCGCTGCGC TGACTCAAGC AATCCGGACA 
GGGACAAAAA CTGGTAAGAA AACATTACAG GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA 
GATGGGACTA TAAAACAACA CAAAGAAGCA AAGAGATTTG AAATTGCAAG ATCAAGTAGC 
AAAGAGGACA ATGCCAAATA CGGCGCAGAA GCAGTGGGAC TTGAGACGGA CATTCAGGCG 
TCACGGACAG ATGAGATTAA AAGAACCACC TACAAGATTC TAGACAGTCT TGAGCAAACC 
ATAAAGCAGC AGGAAAACCT GATGAGCGAA ATGGCACCCA TAATAGGGGC AGAGGCTTTA 
GAATCTAGAG AGAAATGTAA AGTCTCCAGT GACATCAAGA CTGGTGCAGC GGAACCAGTG 
GAAGAACCAA AACCTGTTGT AGAGACTCCT TCCTCCATCC CTACTGCAGC ACGTAAGGGT 
TCCAGTGGAG GAGCACAAAC CAGCAGAATG CCAATCCCAA TGTCTTCTAA AAGTAGGCAA 
GCAAACGTGG ACAAAAGTAG CAAACAGCCC AAACTGCAGG AGCCGCAGCG CCAATACAGA 
CAGGCTAACG GAGGTACTAA GAAAGCTGGT GGGGATGTTA AGGCTTCTGC CCCTTCTTTA 
TCTGTTTCTA AGATTCCAGG CCTTTCCTCT AACACTGGGA AAAGTGGCTC TGTGCCTGCT 
CAGTCTAGCG AGACCTCTAA CCCCCCTAAT CCCTCTGTCA AGTCACATGT TGCCAATTCT 
AACCCCCAAG CTAGCCGCAC CGCTAACCCT TCAGCCCTTG TACGTCCTGC TCACAATGGC 
TCATCCAAAC TTCAGCCCCC GCCTACATAT AAAGGTCACC ATCTTTCATT CTCACCGCAG 
GCCTCAAATG GCAGACCAGC TCTTCCCTCT ACTACTGCTT CTAGTTCTTC TCCCCCTTCT 
TCTGTCTCTC CCACTTCACT GACTCAAGGT GTGAAGAGCA TCCGCACAAT CCATACACCT 
AGCTTCACCA GCTACAAGTC ACAGAACGGA AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG 
ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915500.1
CDS	232..5220
Translation	MQTELRDHGKGTVNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_004915443.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915443.3

ORF Insert Sequence:

ATGCAGACAG AACTTAGGGA CCATGGAAAA GGCACTGTGA ACATACAAGA CAGATGCTTC 
CTCAAAGTCT ACAATAAAGA CCCTGCGCAT GCATTTAACC ACACTCGTGG GGCGGTCAAT 
GGAGATGTAA GGATGCAACG AGATATTTCC TACTCGGTTC GGGAAGGCCC GGCGATCCAT 
AGGCCTGGCT CTGCTACATA CCCCTCCCAT GCTGGCCCAG TGTCACCCCC TCCCACACCT 
GTTCCCCACT CCATGCCATC ATCTCCATCA AGAATCCCCT ATGGCGGTGG GAGGCCTGCA 
GTGGTACAGA GCAACACCAT GCAGAGGGAA AGAATACCAA GCGTGCCGGC ACCTAGATCC 
ATATCTCCTA GCCCCAGTGC TATTTTGGAA AGGAGAGACG TAAAACCAGA TGAGGACATG 
GGCAATAAAA ATGTCCAGCT GCTAAGGAAT GACAGCATTT ACCAAGACCC TTATTTATAC 
CATGAAGGAC GGATGAGTAT GGCATCATCA CATGGTGGGC ACCCTCTCGA CGTCCCTGAT 
CATATTATTG CATACCACAG AGGGTCCTTG AGATCTGCAA GCACTTATTC TAATTCTTCT 
ATGCAAATGG AGATGATGGA ACAATCCATG TACAGACAAA AAAGCCGTAA GCACGTAGAG 
AGTCACTTAC CGACACTTGG TTCTAAAACT CCCCCAACCT CTCCTCACAG AGTGGCTGAT 
ATAAGGATGA TGGAGATGCA TGCCCATAAT GCCCATCTGC CTCCCCACGG CATGCAGGCC 
GACAGATCTT CTCCTCTCCG TCAGTCATTT AAAAAGGAGC AAGGGCCTGG CATGTTTGTA 
GAAGCTAAAG TGCGAAACAC AGGTGGAATA ATGGGCATGG CGGACATTGT CCCATCTCCT 
ACCGATAAGC AAGTATTTGG AGGGTATGGA GTAGCTGTGC CACCAAAAGA CCCCTATACA 
AGAGAGAGGA TGCATGCCAT GGAGAAACAG ATTGCCAGTT TAACTGGTCT TGTTCAGACT 
GCACTTTTGA AAGGGCCCAG CCCAAACAAC TCTGCACCTA GCGACAGAGT CTTAAAATCA 
GCCAACGGCA GCGCTTCAAG CTCAGATCGG CCAGGAAATC AAGGTGTTGC TGCTGCAAAG 
AATTCATTGG CCATTTCAGA AACAATAACT GTTATCAACC AGCAGTCGTC TACCTGCTCG 
CAGGAAATGC ACGTCAGCCT ACAGGACATG AGGCGCAATG TGGCCGAACT TCGTCTTCAG 
CTGCATAAGA TGAGACAACT GCAGCTGCAG AACCAGGAGA CGGTGAGGCT GATGATGAAG 
AAAGCGGAGA TGGAAATCAG CAGCAAAGTG ACTGAGATGG TCAAGGGCCT GGAGGACCCG 
GTGCAGAGAC AGCGCGTCTT GGTGGAACAA GAGCGCCAGA AGTACCTCGG TGACGAGGAA 
CAAATTGTTG CAAAGTTGTG TGACCTTGAA GACTTTGTGG AATCCCTTAA AAAGGATCAG 
AGCACATCCC AGAGGTCGAT CACCCTAAAG GACGTTGAGG ATCGGGCCTT CATGCTGCGC 
CAGATAGGAG AGGCTGTTTC CAACCTGAAA GGAGAGTTTC CGTACCTCCA GAATAAGATG 
CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC GTCAGATTTC TGAAAGAGGA GCCCCACAAA 
CTGGACAGCT TACTGAAAAG GATCCGAAAC ATGACAGATG CCCTTTCTGC CTTAAGAAGA 
CACGCCACTG ACGGATCCTC CAAATCCATT GACAACAATC AGACCAGCCA GGTTCTAATA 
ACAGAAAGAG TAACTGAGAC TGATTCACTC CAGGTCCATG AGGAAAAGTC CCAAGTGTAC 
CTTCAGCCAA CACAAACTGA TGGAGGAGCA GCAGTGGAAT CGCCGACCAC ATCTGTTAAA 
TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC AGAGTCCATC AAGTTCAAAG TTCTCCGGTT 
CTCATGCAGC AGTCCCAGCA CTCGTCTGCA CTCGTGAACC ACACTCCTGC CCCCAGTACC 
CAGAGCAGTC ACAGCACAGA ATTACTAGTG AGCCATCAAA CTGCCAGCGT GCCACCCAGC 
GTCACCCCAG AAAGTGCAAG GAGGAACCCA CCGACGGCCG CACAGGAAGG ATCTTCCAAG 
CAAAGTCTTT TTATTGAGGA AATGAGTCAA GTGACTTACA GGAACCGAGC GGTGTCCATT 
GAGGAGGCAG AGAGAAAATT TGAAGAGAAG AGACAGAATC TTGATCATTA TAACGGAAAG 
GAATTTGAGA AAATGCTGGA AGAAGCACAA GCCAGTATCA TGAAGGCCAT TCCCAGCTTA 
GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG ATTGTGGACA AAAAAGAGGT CACGGAGGTA 
AAAACCATAA TGGAGCAGGA TAACGATAAA GTAACAAAGT CTCCCCCTCC ACCACCACCT 
CGTCGTATGT ACCCACCAGG ATCTGGACTG AACAGTGTAT GGACAGCAGA ATCAACGTTC 
TTGGTCAGGA AGGACAGCAG CAAGGATTCG GAGGAAGCCC TTCCTAAGGT GCCATCAAAG 
GCAGAAACTG AAGAGCCTCA TCTTCAGTCG GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA 
AAGGATGAGG ATGAAGAAGA GGAAGGAGCA AGGATTATGG CTGAACTTCA GGCCTTCCAG 
AAGTGTTCTT TTGTGGATGT AAATCCTAAA AGCCCAAATG AGCTTTCTAA AGCAGATAGC 
CAAGATTACA GAGCTACAGC TGTAACCCAA GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT 
TTAAATGCTG AAAAGCCAGA AGAAAAGTGG AAAATACAAA ATCCAGCATC TGAATCTGCA 
AAAATTAGGC AGGGAGATAC TGTAATTGTG AATGAGACCT TCAGTCTGTT TACAGGAAGG 
GAGAAATATG GTGAGTGCCA AACTGATAAA GAGATCTTAC CTGCCCAGGA ATGTGAGAAA 
GGAGATGGGT CCCCACTTGT GAGCCCAGCA ATCCTTGAGA AGAGACTCAT CAGTTTTAGC 
ACATCAAAAG AAACAGAGAG TGGGATCCCT CAACCCAAAA GAGAAGTGTT AAATGTCAAA 
GTTGGGGAAA TTTTGCATGT AGAAAAGAAG GAAGAGGTGC TAGAAAGTCA CATGACAGCT 
TTAAAGTCAT TAAATGAGCA TGAAATGCCT TATATGGACA TGGGCCAAAC TGTTGTATTG 
AGACCTAAGT CATCGAGAAG AAGTCTTAGT CAACAACAGT CTGATGACTC CAATTCTCCA 
ACTACGTCAC CGACTGAAAC AACATCTCCA GCAGATAATA TTGCGTTCAT GATAACTAAA 
ACTAAAGTCC AGGTGCTTTC AACTGGGGAG GTTCAGGACA TTGTCAGCCG TAAAGGAGAA 
GATGTACAAA CCTTCAACAT TGATAGAGGG AATAACGAGA AAATACTCTC TGGGAATTTA 
GAAAACATTG GATCTGAAGA CCCAGTGGTC TGCACTGACA AGAAACCAGT CATCATCATA 
TTTGATGAAC CAATGGATAT TAAATCAGCA TATAAGAGGC TTTCAACAAT ATTTGAAGAA 
GGTGATGACG ATCTAGAAAA ACACATGTCA GAGGCTAAAA TAGACGAAGA AGATGAAGAA 
GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA TCGAAAAAGG AGTTACCAAT GGAACATACC 
ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG GAAAACAAGT ACAGATTCAA CTATCCAACT 
TTAGCCGAAA TGAAAAAGAC CATCTTCGAA GACGCCAATG CCATACAGAC CCATTTCACA 
GAGGAAGACA GGTCTGAAGC TTCCGATGAT CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA 
AAATTCAAGT TTAAATTTCC CAAAAAACAG CTCGCTGCGC TGACTCAAGC AATCCGGACA 
GGGACAAAAA CTGGTAAGAA AACATTACAG GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA 
GATGGGACTA TAAAACAACA CAAAGAAGCA AAGAGATTTG AAATTGCAAG ATCAAGTAGC 
AAAGAGGACA ATGCCAAATA CGGCGCAGAA GCAGTGGGAC TTGAGACGGA CATTCAGGCG 
TCACGGACAG ATGAGATTAA AAGAACCACC TACAAGATTC TAGACAGTCT TGAGCAAACC 
ATAAAGCAGC AGGAAAACCT GATGAGCGAA ATGGCACCCA TAATAGGGGC AGAGGCTTTA 
GAATCTAGAG AGAAATGTAA AGTCTCCAGT GACATCAAGA CTGGTGCAGC GGAACCAGTG 
GAAGAACCAA AACCTGTTGT AGAGACTCCT TCCTCCATCC CTACTGCAGC ACGTAAGGGT 
TCCAGTGGAG GAGCACAAAC CAGCAGAATG CCAATCCCAA TGTCTTCTAA AAGTAGGCAA 
GCAAACGTGG ACAAAAGTAG CAAACAGCCC AAACTGCAGG AGCCGCAGCG CCAATACAGA 
CAGGCTAACG GAGGTACTAA GAAAGCTGGT GGGGATGTTA AGGCTTCTGC CCCTTCTTTA 
TCTGTTTCTA AGATTCCAGG CCTTTCCTCT AACACTGGGA AAAGTGGCTC TGTGCCTGCT 
CAGTCTAGCG AGACCTCTAA CCCCCCTAAT CCCTCTGTCA AGTCACATGT TGCCAATTCT 
AACCCCCAAG CTAGCCGCAC CGCTAACCCT TCAGCCCTTG TACGTCCTGC TCACAATGGC 
TCATCCAAAC TTCAGCCCCC GCCTACATAT AAAGGTCACC ATCTTTCATT CTCACCGCAG 
GCCTCAAATG GCAGACCAGC TCTTCCCTCT ACTACTGCTT CTAGTTCTTC TCCCCCTTCT 
TCTGTCTCTC CCACTTCACT GACTCAAGGT GTGAAGAGCA TCCGCACAAT CCATACACCT 
AGCTTCACCA GCTACAAGTC ACAGAACGGA AGTGCAAGCA AAGGCCTGAA TGCCAAGGAG 
ACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965644.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821098.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.

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CloneID	OXa36492
Clone ID Related Accession (Same CDS sequence)	XM_012965644.2 , XM_012965644.3
Accession Version	XM_012965644.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821098.1
CDS	292..6111
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965644.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965644.2

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGCGACAGAG TCTTAAAATC AGCCAACGGC 
AGCGCTTCAA GCTCAGATCG GCCAGGAAAT CAAGGTGTTG CTGCTGCAAA GAATTCATTG 
GCCATTTCAG AAACAATAAC TGTTATCAAC CAGCAGTCGT CTACCTGCTC GCAGGAAATG 
CACGTCAGCC TACAGGACAT GAGGCGCAAT GTGGCCGAAC TTCGTCTTCA GCTGCATAAG 
ATGAGACAAC TGCAGCTGCA GAACCAGGAG ACGGTGAGGC TGATGATGAA GAAAGCGGAG 
ATGGAAATCA GCAGCAAAGT GACTGAGATG GTCAAGGGCC TGGAGGACCC GGTGCAGAGA 
CAGCGCGTCT TGGTGGAACA AGAGCGCCAG AAGTACCTCG GTGACGAGGA ACAAATTGTT 
GCAAAGTTGT GTGACCTTGA AGACTTTGTG GAATCCCTTA AAAAGGATCA GAGCACATCC 
CAGAGGTCGA TCACCCTAAA GGACGTTGAG GATCGGGCCT TCATGCTGCG CCAGATAGGA 
GAGGCTGTTT CCAACCTGAA AGGAGAGTTT CCGTACCTCC AGAATAAGAT GCGGGCCGTC 
TTGCGTGTGG AGGTGGAGGC CGTCAGATTT CTGAAAGAGG AGCCCCACAA ACTGGACAGC 
TTACTGAAAA GGATCCGAAA CATGACAGAT GCCCTTTCTG CCTTAAGAAG ACACGCCACT 
GACGGATCCT CCAAATCCAT TGACAACAAT CAGACCAGCC AGGTTCTAAT AACAGAAAGA 
GTAACTGAGA CTGATTCACT CCAGGTCCAT GAGGAAAAGT CCCAAGTGTA CCTTCAGCCA 
ACACAAACTG ATGGAGGAGC AGCAGTGGAA TCGCCGACCA CATCTGTTAA ATCGGAAGTG 
GTTCCCCTGT CGGCCGGCCT CAGAGTCCAT CAAGTTCAAA GTTCTCCGGT TCTCATGCAG 
CAGTCCCAGC ACTCGTCTGC ACTCGTGAAC CACACTCCTG CCCCCAGTAC CCAGAGCAGT 
CACAGCACAG AATTACTAGT GAGCCATCAA ACTGCCAGCG TGCCACCCAG CGTCACCCCA 
GAAAGTGCAA GGAGGAACCC ACCGACGGCC GCACAGGAAG GATCTTCCAA GCAAAGTCTT 
TTTATTGAGG AAATGAGTCA AGTGACTTAC AGGAACCGAG CGGTGTCCAT TGAGGAGGCA 
GAGAGAAAAT TTGAAGAGAA GAGACAGAAT CTTGATCATT ATAACGGAAA GGAATTTGAG 
AAAATGCTGG AAGAAGCACA AGCCAGTATC ATGAAGGCCA TTCCCAGCTT AGAGGTGCCA 
GCCCAGTCCC AGAAAGTGGA GATTGTGGAC AAAAAAGAGG TCACGGAGGT AAAAACCATA 
ATGGAGCAGG ATAACGATAA AGTAACAAAG TCTCCCCCTC CACCACCACC TCGTCGTATG 
TACCCACCAG GATCTGGACT GAACAGTGTA TGGACAGCAG AATCAACGTT CTTGGTCAGG 
AAGGACAGCA GCAAGGATTC GGAGGAAGCC CTTCCTAAGG TGCCATCAAA GGCAGAAACT 
GAAGAGCCTC ATCTTCAGTC GGTGGAGGCT ACAGTCAAGT CTTCTGCCGT AAAGGATGAG 
GATGAAGAAG AGGAAGGAGC AAGGATTATG GCTGAACTTC AGGCCTTCCA GAAGTGTTCT 
TTTGTGGATG TAAATCCTAA AAGCCCAAAT GAGCTTTCTA AAGCAGATAG CCAAGATTAC 
AGAGCTACAG CTGTAACCCA AGCTAAAGAG AAGAAGGTTT CAAAAGAGGG TTTAAATGCT 
GAAAAGCCAG AAGAAAAGTG GAAAATACAA AATCCAGCAT CTGAATCTGC AAAAATTAGG 
CAGGGAGATA CTGTAATTGT GAATGAGACC TTCAGTCTGT TTACAGGAAG GGAGAAATAT 
GGTGAGTGCC AAACTGATAA AGAGATCTTA CCTGCCCAGG AATGTGAGAA AGGAGATGGG 
TCCCCACTTG TGAGCCCAGC AATCCTTGAG AAGAGACTCA TCAGTTTTAG CACATCAAAA 
GAAACAGAGA GTGGGATCCC TCAACCCAAA AGAGAAGTGT TAAATGTCAA AGTTGGGGAA 
ATTTTGCATG TAGAAAAGAA GGAAGAGGTG CTAGAAAGTC ACATGACAGC TTTAAAGTCA 
TTAAATGAGC ATGAAATGCC TTATATGGAC ATGGGCCAAA CTGTTGTATT GAGACCTAAG 
TCATCGAGAA GAAGTCTTAG TCAACAACAG TCTGATGACT CCAATTCTCC AACTACGTCA 
CCGACTGAAA CAACATCTCC AGCAGATAAT ATTGCGTTCA TGATAACTAA AACTAAAGTC 
CAGGTGCTTT CAACTGGGGA GGTTCAGGAC ATTGTCAGCC GTAAAGGAGA AGATGTACAA 
ACCTTCAACA TTGATAGAGG GAATAACGAG AAAATACTCT CTGGGAATTT AGAAAACATT 
GGATCTGAAG ACCCAGTGGT CTGCACTGAC AAGAAACCAG TCATCATCAT ATTTGATGAA 
CCAATGGATA TTAAATCAGC ATATAAGAGG CTTTCAACAA TATTTGAAGA AGGTGATGAC 
GATCTAGAAA AACACATGTC AGAGGCTAAA ATAGACGAAG AAGATGAAGA AGAGGAAGCA 
GAAGTTCCTT CACCCCAAGA ATCGAAAAAG GAGTTACCAA TGGAACATAC CACAAAAGCA 
TCCTCGGAGA ATCCAGGTTT GGAAAACAAG TACAGATTCA ACTATCCAAC TTTAGCCGAA 
ATGAAAAAGA CCATCTTCGA AGACGCCAAT GCCATACAGA CCCATTTCAC AGAGGAAGAC 
AGGTCTGAAG CTTCCGATGA TCAGCTTGGT CAAAGGCAGG ATGTCAAGAA AAAATTCAAG 
TTTAAATTTC CCAAAAAACA GCTCGCTGCG CTGACTCAAG CAATCCGGAC AGGGACAAAA 
ACTGGTAAGA AAACATTACA GGTTGTGGTC TACGAAGAGG AAGAAGAAGC AGATGGGACT 
ATAAAACAAC ACAAAGAAGC AAAGAGATTT GAAATTGCAA GATCAAGTAG CAAAGAGGAC 
AATGCCAAAT ACGGCGCAGA AGCAGTGGGA CTTGAGACGG ACATTCAGGC GTCACGGACA 
GATGAGATTA AAAGAACCAC CTACAAGATT CTAGACAGTC TTGAGCAAAC CATAAAGCAG 
CAGGAAAACC TGATGAGCGA AATGGCACCC ATAATAGGGG CAGAGGCTTT AGAATCTAGA 
GAGAAATGTA AAGTCTCCAG TGACATCAAG ACTGGTGCAG CGGAACCAGT GGAAGAACCA 
AAACCTGTTG TAGAGACTCC TTCCTCCATC CCTACTGCAG CACGTAAGGG TTCCAGTGGA 
GGAGCACAAA CCAGCAGAAT GCCAATCCCA ATGTCTTCTA AAAGTAGGCA AGCAAACGTG 
GACAAAAGTA GCAAACAGCC CAAACTGCAG GAGCCGCAGC GCCAATACAG ACAGGCTAAC 
GGAGGTACTA AGAAAGCTGG TGGGGATGTT AAGGCTTCTG CCCCTTCTTT ATCTGTTTCT 
AAGATTCCAG GCCTTTCCTC TAACACTGGG AAAAGTGGCT CTGTGCCTGC TCAGTCTAGC 
GAGACCTCTA ACCCCCCTAA TCCCTCTGTC AAGTCACATG TTGCCAATTC TAACCCCCAA 
GCTAGCCGCA CCGCTAACCC TTCAGCCCTT GTACGTCCTG CTCACAATGG CTCATCCAAA 
CTTCAGCCCC CGCCTACATA TAAAGGTCAC CATCTTTCAT TCTCACCGCA GGCCTCAAAT 
GGCAGACCAG CTCTTCCCTC TACTACTGCT TCTAGTTCTT CTCCCCCTTC TTCTGTCTCT 
CCCACTTCAC TGACTCAAGG TGTGAAGAGC ATCCGCACAA TCCATACACC TAGCTTCACC 
AGCTACAAGT CACAGAACGG AAGTGCAAGC AAAGGCCTGA ATGCCAAGGA GACCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965645.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821099.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.

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CloneID	OXa36493
Clone ID Related Accession (Same CDS sequence)	XM_012965645.2 , XM_012965645.3
Accession Version	XM_012965645.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGGTTCCAG TGGAGGAGCA CAAACCAGCA GAATGCCAAT 
CCCAATGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821099.1
CDS	296..5650
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARFQWRSTNQQNANPNVF

Target ORF information:

RefSeq Version	XM_012965645.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965645.2

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGTTTC AAAAGAGGGT TTAAATGCTG AAAAGCCAGA AGAAAAGTGG 
AAAATACAAA ATCCAGCATC TGAATCTGCA AAAATTAGGC AGGGAGATAC TGTAATTGTG 
AATGAGACCT TCAGTCTGTT TACAGGAAGG GAGAAATATG GTGAGTGCCA AACTGATAAA 
GAGATCTTAC CTGCCCAGGA ATGTGAGAAA GGAGATGGGT CCCCACTTGT GAGCCCAGCA 
ATCCTTGAGA AGAGACTCAT CAGTTTTAGC ACATCAAAAG AAACAGAGAG TGGGATCCCT 
CAACCCAAAA GAGAAGTGTT AAATGTCAAA GTTGGGGAAA TTTTGCATGT AGAAAAGAAG 
GAAGAGGTGC TAGAAAGTCA CATGACAGCT TTAAAGTCAT TAAATGAGCA TGAAATGCCT 
TATATGGACA TGGGCCAAAC TGTTGTATTG AGACCTAAGT CATCGAGAAG AAGTCTTAGT 
CAACAACAGT CTGATGACTC CAATTCTCCA ACTACGTCAC CGACTGAAAC AACATCTCCA 
GCAGATAATA TTGCGTTCAT GATAACTAAA ACTAAAGTCC AGGTGCTTTC AACTGGGGAG 
GTTCAGGACA TTGTCAGCCG TAAAGGAGAA GATGTACAAA CCTTCAACAT TGATAGAGGG 
AATAACGAGA AAATACTCTC TGGGAATTTA GAAAACATTG GATCTGAAGA CCCAGTGGTC 
TGCACTGACA AGAAACCAGT CATCATCATA TTTGATGAAC CAATGGATAT TAAATCAGCA 
TATAAGAGGC TTTCAACAAT ATTTGAAGAA GGTGATGACG ATCTAGAAAA ACACATGTCA 
GAGGCTAAAA TAGACGAAGA AGATGAAGAA GAGGAAGCAG AAGTTCCTTC ACCCCAAGAA 
TCGAAAAAGG AGTTACCAAT GGAACATACC ACAAAAGCAT CCTCGGAGAA TCCAGGTTTG 
GAAAACAAGT ACAGATTCAA CTATCCAACT TTAGCCGAAA TGAAAAAGAC CATCTTCGAA 
GACGCCAATG CCATACAGAC CCATTTCACA GAGGAAGACA GGTCTGAAGC TTCCGATGAT 
CAGCTTGGTC AAAGGCAGGA TGTCAAGAAA AAATTCAAGT TTAAATTTCC CAAAAAACAG 
CTCGCTGCGC TGACTCAAGC AATCCGGACA GGGACAAAAA CTGGTAAGAA AACATTACAG 
GTTGTGGTCT ACGAAGAGGA AGAAGAAGCA GATGGGACTA TAAAACAACA CAAAGAAGCA 
AAGAGATTTG AAATTGCAAG ATCAAGTAGC AAAGAGGACA ATGCCAAATA CGGCGCAGAA 
GCAGTGGGAC TTGAGACGGA CATTCAGGCG TCACGGACAG ATGAGATTAA AAGAACCACC 
TACAAGATTC TAGACAGTCT TGAGCAAACC ATAAAGCAGC AGGAAAACCT GATGAGCGAA 
ATGGCACCCA TAATAGGGGC AGAGGCTTTA GAATCTAGAG AGAAATGTAA AGTCTCCAGT 
GACATCAAGA CTGGTGCAGC GGAACCAGTG GAAGAACCAA AACCTGTTGT AGAGACTCCT 
TCCTCCATCC CTACTGCAGC ACGGTTCCAG TGGAGGAGCA CAAACCAGCA GAATGCCAAT 
CCCAATGTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965646.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821100.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.

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CloneID	OXa36494
Clone ID Related Accession (Same CDS sequence)	XM_012965646.3 , XM_012965646.2
Accession Version	XM_012965646.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGGCCGTG 
CAGTGCATGT TGGCCACACT TCATTCTGAA CTTGACATTC AAAGGTACTT GATGAAAATT 
GAATCCTCTC AGAGAACAAA AAGTCCGAAG CTGTCTTCAA GCCCACAGCC GAGCGTAGGA 
GACCCGGCAG AACATCTGTC AGAGGCGTCT GCTGATTCCG GAGACATGAT GTTCGAAGCG 
GATTCGGCAG TGCCGTTTGT CCGAGGCAGT CGCACAAGAG CCAGCCTTCC GGTGGTCAGA 
TCCACAAATC AAACGAAAGA GAGATCACTT GGTACGCTCT TCCTACAGTA TGGCGACGAT 
ACCAAGAAAA TGCAGATGCC CAATGAAATC ACAAGCGTAG ACACGATCCG TGCCCTGTTC 
GTAAGTGCCT TCCCCCAGCA ACTGACAATG AAGATCCTGG AATCGCCCAG CACAGCCATT 
TATATTAAGG ATGAGGGCCG AAATGTCTAT TATGAGCTCA GCGATGTCAG GAACATACAA 
GACAGATGCT TCCTCAAAGT CTACAATAAA GACCCTGCGC ATGCATTTAA CCACACTCGT 
GGGGCGGTCA ATGGAGATGT AAGGATGCAA CGAGATATTT CCTACTCGGT TCGGGAAGGC 
CCGGCGATCC ATAGGCCTGG CTCTGCTACA TACCCCTCCC ATGCTGGCCC AGTGTCACCC 
CCTCCCACAC CTGTTCCCCA CTCCATGCCA TCATCTCCAT CAAGAATCCC CTATGGCGGT 
GGGAGGCCTG CAGTGGTACA GAGCAACACC ATGCAGAGGG AAAGAATACC AAGCGTGCCG 
GCACCTAGAT CCATATCTCC TAGCCCCAGT GCTATTTTGG AAAGGAGAGA CGTAAAACCA 
GATGAGGACA TGGGCAATAA AAATGTCCAG CTGCTAAGGA ATGACAGCAT TTACCAAGAC 
CCTTATTTAT ACCATGAAGG ACGGATGAGT ATGGCATCAT CACATGGTGG GCACCCTCTC 
GACGTCCCTG ATCATATTAT TGCATACCAC AGAGGGTCCT TGAGATCTGC AAGCACTTAT 
TCTAATTCTT CTATGCAAAT GGAGATGATG GAACAATCCA TGTACAGACA AAAAAGCCGT 
AAGCACGTAG AGAGTCACTT ACCGACACTT GGTTCTAAAA CTCCCCCAAC CTCTCCTCAC 
AGAGTGGCTG ATATAAGGAT GATGGAGATG CATGCCCATA ATGCCCATCT GCCTCCCCAC 
GGCATGCAGG CCGACAGATC TTCTCCTCTC CGTCAGTCAT TTAAAAAGGA GCAAGGGCCT 
GGCATGTTTG TAGAAGCTAA AGTGCGAAAC ACAGGTGGAA TAATGGGCAT GGCGGACATT 
GTCCCATCTC CTACCGATAA GCAAGTATTT GGAGGGTATG GAGTAGCTGT GCCACCAAAA 
GACCCCTATA CAAGAGAGAG GATGCATGCC ATGGAGAAAC AGATTGCCAG TTTAACTGGT 
CTTGTTCAGA CTGCACTTTT GAAAGGGCCC AGCCCAAACA ACTCTGCACC TAGCGACAGA 
GTCTTAAAAT CAGCCAACGG CAGCGCTTCA AGCTCAGATC GGCCAGGAAA TCAAGGTGTT 
GCTGCTGCAA AGAATTCATT GGCCATTTCA GAAACAATAA CTGTTATCAA CCAGCAGTCG 
TCTACCTGCT CGCAGGAAAT GCACGTCAGC CTACAGGACA TGAGGCGCAA TGTGGCCGAA 
CTTCGTCTTC AGCTGCATAA GATGAGACAA CTGCAGCTGC AGAACCAGGA GACGGTGAGG 
CTGATGATGA AGAAAGCGGA GATGGAAATC AGCAGCAAAG TGACTGAGAT GGTCAAGGGC 
CTGGAGGACC CGGTGCAGAG ACAGCGCGTC TTGGTGGAAC AAGAGCGCCA GAAGTACCTC 
GGTGACGAGG AACAAATTGT TGCAAAGTTG TGTGACCTTG AAGACTTTGT GGAATCCCTT 
AAAAAGGATC AGAGCACATC CCAGAGGTCG ATCACCCTAA AGGACGTTGA GGATCGGGCC 
TTCATGCTGC GCCAGATAGG AGAGGCTGTT TCCAACCTGA AAGGAGAGTT TCCGTACCTC 
CAGAATAAGA TGCGGGCCGT CTTGCGTGTG GAGGTGGAGG CCGTCAGATT TCTGAAAGAG 
GAGCCCCACA AACTGGACAG CTTACTGAAA AGGATCCGAA ACATGACAGA TGCCCTTTCT 
GCCTTAAGAA GACACGCCAC TGACGGATCC TCCAAATCCA TTGACAACAA TCAGACCAGC 
CAGGTTCTAA TAACAGAAAG AGTAACTGAG ACTGATTCAC TCCAGGTCCA TGAGGAAAAG 
TCCCAAGTGT ACCTTCAGCC AACACAAACT GATGGAGGAG CAGCAGTGGA ATCGCCGACC 
ACATCTGTTA AATCGGAAGT GGTTCCCCTG TCGGCCGGCC TCAGAGTCCA TCAAGTTCAA 
AGTTCTCCGG TTCTCATGCA GCAGTCCCAG CACTCGTCTG CACTCGTGAA CCACACTCCT 
GCCCCCAGTA CCCAGAGCAG TCACAGCACA GAATTACTAG TGAGCCATCA AACTGCCAGC 
GTGCCACCCA GCGTCACCCC AGAAAGTGCA AGGAGGAACC CACCGACGGC CGCACAGGAA 
GGATCTTCCA AGCAAAGTCT TTTTATTGAG GAAATGAGTC AAGTGACTTA CAGGAACCGA 
GCGGTGTCCA TTGAGGAGGC AGAGAGAAAA TTTGAAGAGA AGAGACAGAA TCTTGATCAT 
TATAACGGAA AGGAATTTGA GAAAATGCTG GAAGAAGCAC AAGCCAGTAT CATGAAGGCC 
ATTCCCAGCT TAGAGGTGCC AGCCCAGTCC CAGAAAGTGG AGATTGTGGA CAAAAAAGAG 
GTCACGGAGG TAAAAACCAT AATGGAGCAG GATAACGATA AAGTAACAAA GTCTCCCCCT 
CCACCACCAC CTCGTCGTAT GTACCCACCA GGATCTGGAC TGAACAGTGT ATGGACAGCA 
GAATCAACGT TCTTGGTCAG GAAGGACAGC AGCAAGGATT CGGAGGAAGC CCTTCCTAAG 
GTGCCATCAA AGGCAGAAAC TGAAGAGCCT CATCTTCAGT CGGTGGAGGC TACAGTCAAG 
TCTTCTGCCG TAAAGGATGA GGATGAAGAA GAGGAAGGAG CAAGGATTAT GGCTGAACTT 
CAGGCCTTCC AGAAGTGTTC TTTTGTGGAT GTAAATCCTA AAAGCCCAAA TGAGCTTTCT 
AAAGCAGATA GCCAAGATTA CAGAGCTACA GCTGTAACCC AAGCTAAAGA GAAGAAGGTT 
TCAAAAGAGG GTTTAAATGC TGAAAAGCCA GAAGAAAAGT GGAAAATACA AAATCCAGCA 
TCTGAATCTG CAAAAATTAG GCAGGGAGAT ACTGTAATTG TGAATGAGAC CTTCAGTCTG 
TTTACAGGAA GGGAGAAATA TGGTGAGTGC CAAACTGATA AAGAGATCTT ACCTGCCCAG 
GAATGTGAGA AAGGAGATGG GTCCCCACTT GTGAGCCCAG CAATCCTTGA GAAGAGACTC 
ATCAGTTTTA GCACATCAAA AGAAACAGAG AGTGGGATCC CTCAACCCAA AAGAGAAGTG 
TTAAATGTCA AAGTTGGGGA AATTTTGCAT GTAGAAAAGA AGGAAGAGGT GCTAGAAAGT 
CACATGACAG CTTTAAAGTC ATTAAATGAG CATGAAATGC CTTATATGGA CATGGGCCAA 
ACTGTTGTAT TGAGACCTAA GTCATCGAGA AGAAGTCTTA GTCAACAACA GTCTGATGAC 
TCCAATTCTC CAACTACGTC ACCGACTGAA ACAACATCTC CAGCAGATAA TATTGCGTTC 
ATGATAACTA AAACTAAAGT CCAGGTGCTT TCAACTGGGG AGGTTCAGGA CATTGTCAGC 
CGTAAAGGAG AAGATGTACA AACCTTCAAC ATTGATAGAG GGAATAACGA GAAAATACTC 
TCTGGGAATT TAGAAAACAT TGGATCTGAA GACCCAGTGG TCTGCACTGA CAAGAAACCA 
GTCATCATCA TATTTGATGA ACCAATGGAT ATTAAATCAG CATATAAGAG GCTTTCAACA 
ATATTTGAAG AAGGTGATGA CGATCTAGAA AAACACATGT CAGAGGCTAA AATAGACGAA 
GAAGATGAAG AAGAGGAAGC AGAAGTTCCT TCACCCCAAG AATCGAAAAA GGAGTTACCA 
ATGGAACATA CCACAAAAGC ATCCTCGGAG AATCCAGGTT TGGAAAACAA GTACAGATTC 
AACTATCCAA CTTTAGCCGA AATGAAAAAG ACCATCTTCG AAGACGCCAA TGCCATACAG 
ACCCATTTCA CAGAGGAAGA CAGGTCTGAA GCTTCCGATG ATCAGCTTGG TCAAAGGCAG 
GATGTCAAGA AAAAATTCAA GTTTAAATTT CCCAAAAAAC AGCTCGCTGC GCTGACTCAA 
GCAATCCGGA CAGGGACAAA AACTGGTAAG AAAACATTAC AGGTTGTGGT CTACGAAGAG 
GAAGAAGAAG CAGATGGGAC TATAAAACAA CACAAAGAAG CAAAGAGATT TGAAATTGCA 
AGATCAAGTA GCAAAGAGGA CAATGCCAAA TACGGCGCAG AAGCAGTGGG ACTTGAGACG 
GACATTCAGG CGTCACGGAC AGATGAGATT AAAAGAACCA CCTACAAGAT TCTAGACAGT 
CTTGAGCAAA CCATAAAGCA GCAGGAAAAC CTGATGAGCG AAATGGCACC CATAATAGGG 
GCAGAGGCTT TAGAATCTAG AGAGAAATGT AAAGTCTCCA GTGACATCAA GACTGGTGCA 
GCGGAACCAG TGGAAGAACC AAAACCTGTT GTAGAGACTC CTTCCTCCAT CCCTACTGCA 
GCACGTAAGG GTTCCAGTGG AGGAGCACAA ACCAGCAGAA TGCCAATCCC AATGTCTTCT 
AAAAGTAGGC AAGCAAACGT GGACAAAAGT AGCAAACAGC CCAAACTGCA GGAGCCGCAG 
CGCCAATACA GACAGGCTAA CGGAGGTACT AAGAAAGCTG GTGGGGATGT TAAGGCTTCT 
GCCCCTTCTT TATCTGTTTC TAAGATTCCA GGCCTTTCCT CTAACACTGG GAAAAGTGGC 
TCTGTGCCTG CTCAGTCTAG CGAGACCTCT AACCCCCCTA ATCCCTCTGT CAAGTCACAT 
GTTGCCAATT CTAACCCCCA AGCTAGCCGC ACCGCTAACC CTTCAGCCCT TGTACGTCCT 
GCTCACAATG GCTCATCCAA ACTTCAGCCC CCGCCTACAT ATAAAGGTCA CCATCTTTCA 
TTCTCACCGC AGGCCTCAAA TGGCAGACCA GCTCTTCCCT CTACTACTGC TTCTAGTTCT 
TCTCCCCCTT CTTCTGTCTC TCCCACTTCA CTGACTCAAG GTGTGAAGAG CATCCGCACA 
ATCCATACAC CTAGCTTCAC CAGCTACAAG TCACAGAACG GAAGTGCAAG CAAAGGCCTG 
AATGCCAAGG AGACCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821100.1
CDS	230..5770
Translation	MQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKVSKEGLNAEKPEEKWKIQNPASESAKIRQGDTVIVNETFSLFTGREKYGECQTDKEILPAQECEKGDGSPLVSPAILEKRLISFSTSKETESGIPQPKREVLNVKVGEILHVEKKEEVLESHMTALKSLNEHEMPYMDMGQTVVLRPKSSRRSLSQQQSDDSNSPTTSPTETTSPADNIAFMITKTKVQVLSTGEVQDIVSRKGEDVQTFNIDRGNNEKILSGNLENIGSEDPVVCTDKKPVIIIFDEPMDIKSAYKRLSTIFEEGDDDLEKHMSEAKIDEEDEEEEAEVPSPQESKKELPMEHTTKASSENPGLENKYRFNYPTLAEMKKTIFEDANAIQTHFTEEDRSEASDDQLGQRQDVKKKFKFKFPKKQLAALTQAIRTGTKTGKKTLQVVVYEEEEEADGTIKQHKEAKRFEIARSSSKEDNAKYGAEAVGLETDIQASRTDEIKRTTYKILDSLEQTIKQQENLMSEMAPIIGAEALESREKCKVSSDIKTGAAEPVEEPKPVVETPSSIPTAARKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965646.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965646.2

ORF Insert Sequence:

ATGCAGACAT CGGAAATGGA CAGGAAAAGA GAGGCTTTTC TAGAACACCT CAAATACAAA 
TATCCTCATC ATGCGACTGC AATTATGGGC CACCAGGAAC GGCTGAGAGA TCAGGCCGTG 
CAGTGCATGT TGGCCACACT TCATTCTGAA CTTGACATTC AAAGGTACTT GATGAAAATT 
GAATCCTCTC AGAGAACAAA AAGTCCGAAG CTGTCTTCAA GCCCACAGCC GAGCGTAGGA 
GACCCGGCAG AACATCTGTC AGAGGCGTCT GCTGATTCCG GAGACATGAT GTTCGAAGCG 
GATTCGGCAG TGCCGTTTGT CCGAGGCAGT CGCACAAGAG CCAGCCTTCC GGTGGTCAGA 
TCCACAAATC AAACGAAAGA GAGATCACTT GGTACGCTCT TCCTACAGTA TGGCGACGAT 
ACCAAGAAAA TGCAGATGCC CAATGAAATC ACAAGCGTAG ACACGATCCG TGCCCTGTTC 
GTAAGTGCCT TCCCCCAGCA ACTGACAATG AAGATCCTGG AATCGCCCAG CACAGCCATT 
TATATTAAGG ATGAGGGCCG AAATGTCTAT TATGAGCTCA GCGATGTCAG GAACATACAA 
GACAGATGCT TCCTCAAAGT CTACAATAAA GACCCTGCGC ATGCATTTAA CCACACTCGT 
GGGGCGGTCA ATGGAGATGT AAGGATGCAA CGAGATATTT CCTACTCGGT TCGGGAAGGC 
CCGGCGATCC ATAGGCCTGG CTCTGCTACA TACCCCTCCC ATGCTGGCCC AGTGTCACCC 
CCTCCCACAC CTGTTCCCCA CTCCATGCCA TCATCTCCAT CAAGAATCCC CTATGGCGGT 
GGGAGGCCTG CAGTGGTACA GAGCAACACC ATGCAGAGGG AAAGAATACC AAGCGTGCCG 
GCACCTAGAT CCATATCTCC TAGCCCCAGT GCTATTTTGG AAAGGAGAGA CGTAAAACCA 
GATGAGGACA TGGGCAATAA AAATGTCCAG CTGCTAAGGA ATGACAGCAT TTACCAAGAC 
CCTTATTTAT ACCATGAAGG ACGGATGAGT ATGGCATCAT CACATGGTGG GCACCCTCTC 
GACGTCCCTG ATCATATTAT TGCATACCAC AGAGGGTCCT TGAGATCTGC AAGCACTTAT 
TCTAATTCTT CTATGCAAAT GGAGATGATG GAACAATCCA TGTACAGACA AAAAAGCCGT 
AAGCACGTAG AGAGTCACTT ACCGACACTT GGTTCTAAAA CTCCCCCAAC CTCTCCTCAC 
AGAGTGGCTG ATATAAGGAT GATGGAGATG CATGCCCATA ATGCCCATCT GCCTCCCCAC 
GGCATGCAGG CCGACAGATC TTCTCCTCTC CGTCAGTCAT TTAAAAAGGA GCAAGGGCCT 
GGCATGTTTG TAGAAGCTAA AGTGCGAAAC ACAGGTGGAA TAATGGGCAT GGCGGACATT 
GTCCCATCTC CTACCGATAA GCAAGTATTT GGAGGGTATG GAGTAGCTGT GCCACCAAAA 
GACCCCTATA CAAGAGAGAG GATGCATGCC ATGGAGAAAC AGATTGCCAG TTTAACTGGT 
CTTGTTCAGA CTGCACTTTT GAAAGGGCCC AGCCCAAACA ACTCTGCACC TAGCGACAGA 
GTCTTAAAAT CAGCCAACGG CAGCGCTTCA AGCTCAGATC GGCCAGGAAA TCAAGGTGTT 
GCTGCTGCAA AGAATTCATT GGCCATTTCA GAAACAATAA CTGTTATCAA CCAGCAGTCG 
TCTACCTGCT CGCAGGAAAT GCACGTCAGC CTACAGGACA TGAGGCGCAA TGTGGCCGAA 
CTTCGTCTTC AGCTGCATAA GATGAGACAA CTGCAGCTGC AGAACCAGGA GACGGTGAGG 
CTGATGATGA AGAAAGCGGA GATGGAAATC AGCAGCAAAG TGACTGAGAT GGTCAAGGGC 
CTGGAGGACC CGGTGCAGAG ACAGCGCGTC TTGGTGGAAC AAGAGCGCCA GAAGTACCTC 
GGTGACGAGG AACAAATTGT TGCAAAGTTG TGTGACCTTG AAGACTTTGT GGAATCCCTT 
AAAAAGGATC AGAGCACATC CCAGAGGTCG ATCACCCTAA AGGACGTTGA GGATCGGGCC 
TTCATGCTGC GCCAGATAGG AGAGGCTGTT TCCAACCTGA AAGGAGAGTT TCCGTACCTC 
CAGAATAAGA TGCGGGCCGT CTTGCGTGTG GAGGTGGAGG CCGTCAGATT TCTGAAAGAG 
GAGCCCCACA AACTGGACAG CTTACTGAAA AGGATCCGAA ACATGACAGA TGCCCTTTCT 
GCCTTAAGAA GACACGCCAC TGACGGATCC TCCAAATCCA TTGACAACAA TCAGACCAGC 
CAGGTTCTAA TAACAGAAAG AGTAACTGAG ACTGATTCAC TCCAGGTCCA TGAGGAAAAG 
TCCCAAGTGT ACCTTCAGCC AACACAAACT GATGGAGGAG CAGCAGTGGA ATCGCCGACC 
ACATCTGTTA AATCGGAAGT GGTTCCCCTG TCGGCCGGCC TCAGAGTCCA TCAAGTTCAA 
AGTTCTCCGG TTCTCATGCA GCAGTCCCAG CACTCGTCTG CACTCGTGAA CCACACTCCT 
GCCCCCAGTA CCCAGAGCAG TCACAGCACA GAATTACTAG TGAGCCATCA AACTGCCAGC 
GTGCCACCCA GCGTCACCCC AGAAAGTGCA AGGAGGAACC CACCGACGGC CGCACAGGAA 
GGATCTTCCA AGCAAAGTCT TTTTATTGAG GAAATGAGTC AAGTGACTTA CAGGAACCGA 
GCGGTGTCCA TTGAGGAGGC AGAGAGAAAA TTTGAAGAGA AGAGACAGAA TCTTGATCAT 
TATAACGGAA AGGAATTTGA GAAAATGCTG GAAGAAGCAC AAGCCAGTAT CATGAAGGCC 
ATTCCCAGCT TAGAGGTGCC AGCCCAGTCC CAGAAAGTGG AGATTGTGGA CAAAAAAGAG 
GTCACGGAGG TAAAAACCAT AATGGAGCAG GATAACGATA AAGTAACAAA GTCTCCCCCT 
CCACCACCAC CTCGTCGTAT GTACCCACCA GGATCTGGAC TGAACAGTGT ATGGACAGCA 
GAATCAACGT TCTTGGTCAG GAAGGACAGC AGCAAGGATT CGGAGGAAGC CCTTCCTAAG 
GTGCCATCAA AGGCAGAAAC TGAAGAGCCT CATCTTCAGT CGGTGGAGGC TACAGTCAAG 
TCTTCTGCCG TAAAGGATGA GGATGAAGAA GAGGAAGGAG CAAGGATTAT GGCTGAACTT 
CAGGCCTTCC AGAAGTGTTC TTTTGTGGAT GTAAATCCTA AAAGCCCAAA TGAGCTTTCT 
AAAGCAGATA GCCAAGATTA CAGAGCTACA GCTGTAACCC AAGCTAAAGA GAAGAAGGTT 
TCAAAAGAGG GTTTAAATGC TGAAAAGCCA GAAGAAAAGT GGAAAATACA AAATCCAGCA 
TCTGAATCTG CAAAAATTAG GCAGGGAGAT ACTGTAATTG TGAATGAGAC CTTCAGTCTG 
TTTACAGGAA GGGAGAAATA TGGTGAGTGC CAAACTGATA AAGAGATCTT ACCTGCCCAG 
GAATGTGAGA AAGGAGATGG GTCCCCACTT GTGAGCCCAG CAATCCTTGA GAAGAGACTC 
ATCAGTTTTA GCACATCAAA AGAAACAGAG AGTGGGATCC CTCAACCCAA AAGAGAAGTG 
TTAAATGTCA AAGTTGGGGA AATTTTGCAT GTAGAAAAGA AGGAAGAGGT GCTAGAAAGT 
CACATGACAG CTTTAAAGTC ATTAAATGAG CATGAAATGC CTTATATGGA CATGGGCCAA 
ACTGTTGTAT TGAGACCTAA GTCATCGAGA AGAAGTCTTA GTCAACAACA GTCTGATGAC 
TCCAATTCTC CAACTACGTC ACCGACTGAA ACAACATCTC CAGCAGATAA TATTGCGTTC 
ATGATAACTA AAACTAAAGT CCAGGTGCTT TCAACTGGGG AGGTTCAGGA CATTGTCAGC 
CGTAAAGGAG AAGATGTACA AACCTTCAAC ATTGATAGAG GGAATAACGA GAAAATACTC 
TCTGGGAATT TAGAAAACAT TGGATCTGAA GACCCAGTGG TCTGCACTGA CAAGAAACCA 
GTCATCATCA TATTTGATGA ACCAATGGAT ATTAAATCAG CATATAAGAG GCTTTCAACA 
ATATTTGAAG AAGGTGATGA CGATCTAGAA AAACACATGT CAGAGGCTAA AATAGACGAA 
GAAGATGAAG AAGAGGAAGC AGAAGTTCCT TCACCCCAAG AATCGAAAAA GGAGTTACCA 
ATGGAACATA CCACAAAAGC ATCCTCGGAG AATCCAGGTT TGGAAAACAA GTACAGATTC 
AACTATCCAA CTTTAGCCGA AATGAAAAAG ACCATCTTCG AAGACGCCAA TGCCATACAG 
ACCCATTTCA CAGAGGAAGA CAGGTCTGAA GCTTCCGATG ATCAGCTTGG TCAAAGGCAG 
GATGTCAAGA AAAAATTCAA GTTTAAATTT CCCAAAAAAC AGCTCGCTGC GCTGACTCAA 
GCAATCCGGA CAGGGACAAA AACTGGTAAG AAAACATTAC AGGTTGTGGT CTACGAAGAG 
GAAGAAGAAG CAGATGGGAC TATAAAACAA CACAAAGAAG CAAAGAGATT TGAAATTGCA 
AGATCAAGTA GCAAAGAGGA CAATGCCAAA TACGGCGCAG AAGCAGTGGG ACTTGAGACG 
GACATTCAGG CGTCACGGAC AGATGAGATT AAAAGAACCA CCTACAAGAT TCTAGACAGT 
CTTGAGCAAA CCATAAAGCA GCAGGAAAAC CTGATGAGCG AAATGGCACC CATAATAGGG 
GCAGAGGCTT TAGAATCTAG AGAGAAATGT AAAGTCTCCA GTGACATCAA GACTGGTGCA 
GCGGAACCAG TGGAAGAACC AAAACCTGTT GTAGAGACTC CTTCCTCCAT CCCTACTGCA 
GCACGTAAGG GTTCCAGTGG AGGAGCACAA ACCAGCAGAA TGCCAATCCC AATGTCTTCT 
AAAAGTAGGC AAGCAAACGT GGACAAAAGT AGCAAACAGC CCAAACTGCA GGAGCCGCAG 
CGCCAATACA GACAGGCTAA CGGAGGTACT AAGAAAGCTG GTGGGGATGT TAAGGCTTCT 
GCCCCTTCTT TATCTGTTTC TAAGATTCCA GGCCTTTCCT CTAACACTGG GAAAAGTGGC 
TCTGTGCCTG CTCAGTCTAG CGAGACCTCT AACCCCCCTA ATCCCTCTGT CAAGTCACAT 
GTTGCCAATT CTAACCCCCA AGCTAGCCGC ACCGCTAACC CTTCAGCCCT TGTACGTCCT 
GCTCACAATG GCTCATCCAA ACTTCAGCCC CCGCCTACAT ATAAAGGTCA CCATCTTTCA 
TTCTCACCGC AGGCCTCAAA TGGCAGACCA GCTCTTCCCT CTACTACTGC TTCTAGTTCT 
TCTCCCCCTT CTTCTGTCTC TCCCACTTCA CTGACTCAAG GTGTGAAGAG CATCCGCACA 
ATCCATACAC CTAGCTTCAC CAGCTACAAG TCACAGAACG GAAGTGCAAG CAAAGGCCTG 
AATGCCAAGG AGACCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965647.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821101.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.

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CloneID	OXa36495
Clone ID Related Accession (Same CDS sequence)	XM_012965647.2 , XM_012965647.3
Accession Version	XM_012965647.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGGTTC CAGTGGAGGA GCACAAACCA GCAGAATGCC AATCCCAATG 
TCTTCTAAAA GTAGGCAAGC AAACGTGGAC AAAAGTAGCA AACAGCCCAA ACTGCAGGAG 
CCGCAGCGCC AATACAGACA GGCTAACGGA GGTACTAAGA AAGCTGGTGG GGATGTTAAG 
GCTTCTGCCC CTTCTTTATC TGTTTCTAAG ATTCCAGGCC TTTCCTCTAA CACTGGGAAA 
AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG ACCTCTAACC CCCCTAATCC CTCTGTCAAG 
TCACATGTTG CCAATTCTAA CCCCCAAGCT AGCCGCACCG CTAACCCTTC AGCCCTTGTA 
CGTCCTGCTC ACAATGGCTC ATCCAAACTT CAGCCCCCGC CTACATATAA AGGTCACCAT 
CTTTCATTCT CACCGCAGGC CTCAAATGGC AGACCAGCTC TTCCCTCTAC TACTGCTTCT 
AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC ACTTCACTGA CTCAAGGTGT GAAGAGCATC 
CGCACAATCC ATACACCTAG CTTCACCAGC TACAAGTCAC AGAACGGAAG TGCAAGCAAA 
GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821101.1
CDS	288..4634
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965647.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965647.2

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGCCTTCCAG AAGTGTTCTT TTGTGGATGT AAATCCTAAA 
AGCCCAAATG AGCTTTCTAA AGCAGATAGC CAAGATTACA GAGCTACAGC TGTAACCCAA 
GCTAAAGAGA AGAAGGGTTC CAGTGGAGGA GCACAAACCA GCAGAATGCC AATCCCAATG 
TCTTCTAAAA GTAGGCAAGC AAACGTGGAC AAAAGTAGCA AACAGCCCAA ACTGCAGGAG 
CCGCAGCGCC AATACAGACA GGCTAACGGA GGTACTAAGA AAGCTGGTGG GGATGTTAAG 
GCTTCTGCCC CTTCTTTATC TGTTTCTAAG ATTCCAGGCC TTTCCTCTAA CACTGGGAAA 
AGTGGCTCTG TGCCTGCTCA GTCTAGCGAG ACCTCTAACC CCCCTAATCC CTCTGTCAAG 
TCACATGTTG CCAATTCTAA CCCCCAAGCT AGCCGCACCG CTAACCCTTC AGCCCTTGTA 
CGTCCTGCTC ACAATGGCTC ATCCAAACTT CAGCCCCCGC CTACATATAA AGGTCACCAT 
CTTTCATTCT CACCGCAGGC CTCAAATGGC AGACCAGCTC TTCCCTCTAC TACTGCTTCT 
AGTTCTTCTC CCCCTTCTTC TGTCTCTCCC ACTTCACTGA CTCAAGGTGT GAAGAGCATC 
CGCACAATCC ATACACCTAG CTTCACCAGC TACAAGTCAC AGAACGGAAG TGCAAGCAAA 
GGCCTGAATG CCAAGGAGAC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965648.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821102.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.

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CloneID	OXa36496
Clone ID Related Accession (Same CDS sequence)	XM_012965648.2 , XM_012965648.3
Accession Version	XM_012965648.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGGTTCCAGT GGAGGAGCAC AAACCAGCAG AATGCCAATC 
CCAATGTCTT CTAAAAGTAG GCAAGCAAAC GTGGACAAAA GTAGCAAACA GCCCAAACTG 
CAGGAGCCGC AGCGCCAATA CAGACAGGCT AACGGAGGTA CTAAGAAAGC TGGTGGGGAT 
GTTAAGGCTT CTGCCCCTTC TTTATCTGTT TCTAAGATTC CAGGCCTTTC CTCTAACACT 
GGGAAAAGTG GCTCTGTGCC TGCTCAGTCT AGCGAGACCT CTAACCCCCC TAATCCCTCT 
GTCAAGTCAC ATGTTGCCAA TTCTAACCCC CAAGCTAGCC GCACCGCTAA CCCTTCAGCC 
CTTGTACGTC CTGCTCACAA TGGCTCATCC AAACTTCAGC CCCCGCCTAC ATATAAAGGT 
CACCATCTTT CATTCTCACC GCAGGCCTCA AATGGCAGAC CAGCTCTTCC CTCTACTACT 
GCTTCTAGTT CTTCTCCCCC TTCTTCTGTC TCTCCCACTT CACTGACTCA AGGTGTGAAG 
AGCATCCGCA CAATCCATAC ACCTAGCTTC ACCAGCTACA AGTCACAGAA CGGAAGTGCA 
AGCAAAGGCC TGAATGCCAA GGAGACCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821102.1
CDS	287..4519
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQAVQCMLATLHSELDIQRYLMKIESSQRTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965648.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965648.2

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGGCCGTGCA GTGCATGTTG GCCACACTTC ATTCTGAACT TGACATTCAA 
AGGTACTTGA TGAAAATTGA ATCCTCTCAG AGAACAAAAA GTCCGAAGCT GTCTTCAAGC 
CCACAGCCGA GCGTAGGAGA CCCGGCAGAA CATCTGTCAG AGGCGTCTGC TGATTCCGGA 
GACATGATGT TCGAAGCGGA TTCGGCAGTG CCGTTTGTCC GAGGCAGTCG CACAAGAGCC 
AGCCTTCCGG TGGTCAGATC CACAAATCAA ACGAAAGAGA GATCACTTGG TACGCTCTTC 
CTACAGTATG GCGACGATAC CAAGAAAATG CAGATGCCCA ATGAAATCAC AAGCGTAGAC 
ACGATCCGTG CCCTGTTCGT AAGTGCCTTC CCCCAGCAAC TGACAATGAA GATCCTGGAA 
TCGCCCAGCA CAGCCATTTA TATTAAGGAT GAGGGCCGAA ATGTCTATTA TGAGCTCAGC 
GATGTCAGGA ACATACAAGA CAGATGCTTC CTCAAAGTCT ACAATAAAGA CCCTGCGCAT 
GCATTTAACC ACACTCGTGG GGCGGTCAAT GGAGATGTAA GGATGCAACG AGATATTTCC 
TACTCGGTTC GGGAAGGCCC GGCGATCCAT AGGCCTGGCT CTGCTACATA CCCCTCCCAT 
GCTGGCCCAG TGTCACCCCC TCCCACACCT GTTCCCCACT CCATGCCATC ATCTCCATCA 
AGAATCCCCT ATGGCGGTGG GAGGCCTGCA GTGGTACAGA GCAACACCAT GCAGAGGGAA 
AGAATACCAA GCGTGCCGGC ACCTAGATCC ATATCTCCTA GCCCCAGTGC TATTTTGGAA 
AGGAGAGACG TAAAACCAGA TGAGGACATG GGCAATAAAA ATGTCCAGCT GCTAAGGAAT 
GACAGCATTT ACCAAGACCC TTATTTATAC CATGAAGGAC GGATGAGTAT GGCATCATCA 
CATGGTGGGC ACCCTCTCGA CGTCCCTGAT CATATTATTG CATACCACAG AGGGTCCTTG 
AGATCTGCAA GCACTTATTC TAATTCTTCT ATGCAAATGG AGATGATGGA ACAATCCATG 
TACAGACAAA AAAGCCGTAA GCACGTAGAG AGTCACTTAC CGACACTTGG TTCTAAAACT 
CCCCCAACCT CTCCTCACAG AGTGGCTGAT ATAAGGATGA TGGAGATGCA TGCCCATAAT 
GCCCATCTGC CTCCCCACGG CATGCAGGCC GACAGATCTT CTCCTCTCCG TCAGTCATTT 
AAAAAGGAGC AAGGGCCTGG CATGTTTGTA GAAGCTAAAG TGCGAAACAC AGGTGGAATA 
ATGGGCATGG CGGACATTGT CCCATCTCCT ACCGATAAGC AAGTATTTGG AGGGTATGGA 
GTAGCTGTGC CACCAAAAGA CCCCTATACA AGAGAGAGGA TGCATGCCAT GGAGAAACAG 
ATTGCCAGTT TAACTGGTCT TGTTCAGACT GCACTTTTGA AAGGGCCCAG CCCAAACAAC 
TCTGCACCTA GCGACAGAGT CTTAAAATCA GCCAACGGCA GCGCTTCAAG CTCAGATCGG 
CCAGGAAATC AAGGTGTTGC TGCTGCAAAG AATTCATTGG CCATTTCAGA AACAATAACT 
GTTATCAACC AGCAGTCGTC TACCTGCTCG CAGGAAATGC ACGTCAGCCT ACAGGACATG 
AGGCGCAATG TGGCCGAACT TCGTCTTCAG CTGCATAAGA TGAGACAACT GCAGCTGCAG 
AACCAGGAGA CGGTGAGGCT GATGATGAAG AAAGCGGAGA TGGAAATCAG CAGCAAAGTG 
ACTGAGATGG TCAAGGGCCT GGAGGACCCG GTGCAGAGAC AGCGCGTCTT GGTGGAACAA 
GAGCGCCAGA AGTACCTCGG TGACGAGGAA CAAATTGTTG CAAAGTTGTG TGACCTTGAA 
GACTTTGTGG AATCCCTTAA AAAGGATCAG AGCACATCCC AGAGGTCGAT CACCCTAAAG 
GACGTTGAGG ATCGGGCCTT CATGCTGCGC CAGATAGGAG AGGCTGTTTC CAACCTGAAA 
GGAGAGTTTC CGTACCTCCA GAATAAGATG CGGGCCGTCT TGCGTGTGGA GGTGGAGGCC 
GTCAGATTTC TGAAAGAGGA GCCCCACAAA CTGGACAGCT TACTGAAAAG GATCCGAAAC 
ATGACAGATG CCCTTTCTGC CTTAAGAAGA CACGCCACTG ACGGATCCTC CAAATCCATT 
GACAACAATC AGACCAGCCA GGTTCTAATA ACAGAAAGAG TAACTGAGAC TGATTCACTC 
CAGGTCCATG AGGAAAAGTC CCAAGTGTAC CTTCAGCCAA CACAAACTGA TGGAGGAGCA 
GCAGTGGAAT CGCCGACCAC ATCTGTTAAA TCGGAAGTGG TTCCCCTGTC GGCCGGCCTC 
AGAGTCCATC AAGTTCAAAG TTCTCCGGTT CTCATGCAGC AGTCCCAGCA CTCGTCTGCA 
CTCGTGAACC ACACTCCTGC CCCCAGTACC CAGAGCAGTC ACAGCACAGA ATTACTAGTG 
AGCCATCAAA CTGCCAGCGT GCCACCCAGC GTCACCCCAG AAAGTGCAAG GAGGAACCCA 
CCGACGGCCG CACAGGAAGG ATCTTCCAAG CAAAGTCTTT TTATTGAGGA AATGAGTCAA 
GTGACTTACA GGAACCGAGC GGTGTCCATT GAGGAGGCAG AGAGAAAATT TGAAGAGAAG 
AGACAGAATC TTGATCATTA TAACGGAAAG GAATTTGAGA AAATGCTGGA AGAAGCACAA 
GCCAGTATCA TGAAGGCCAT TCCCAGCTTA GAGGTGCCAG CCCAGTCCCA GAAAGTGGAG 
ATTGTGGACA AAAAAGAGGT CACGGAGGTA AAAACCATAA TGGAGCAGGA TAACGATAAA 
GTAACAAAGT CTCCCCCTCC ACCACCACCT CGTCGTATGT ACCCACCAGG ATCTGGACTG 
AACAGTGTAT GGACAGCAGA ATCAACGTTC TTGGTCAGGA AGGACAGCAG CAAGGATTCG 
GAGGAAGCCC TTCCTAAGGT GCCATCAAAG GCAGAAACTG AAGAGCCTCA TCTTCAGTCG 
GTGGAGGCTA CAGTCAAGTC TTCTGCCGTA AAGGATGAGG ATGAAGAAGA GGAAGGAGCA 
AGGATTATGG CTGAACTTCA GGGTTCCAGT GGAGGAGCAC AAACCAGCAG AATGCCAATC 
CCAATGTCTT CTAAAAGTAG GCAAGCAAAC GTGGACAAAA GTAGCAAACA GCCCAAACTG 
CAGGAGCCGC AGCGCCAATA CAGACAGGCT AACGGAGGTA CTAAGAAAGC TGGTGGGGAT 
GTTAAGGCTT CTGCCCCTTC TTTATCTGTT TCTAAGATTC CAGGCCTTTC CTCTAACACT 
GGGAAAAGTG GCTCTGTGCC TGCTCAGTCT AGCGAGACCT CTAACCCCCC TAATCCCTCT 
GTCAAGTCAC ATGTTGCCAA TTCTAACCCC CAAGCTAGCC GCACCGCTAA CCCTTCAGCC 
CTTGTACGTC CTGCTCACAA TGGCTCATCC AAACTTCAGC CCCCGCCTAC ATATAAAGGT 
CACCATCTTT CATTCTCACC GCAGGCCTCA AATGGCAGAC CAGCTCTTCC CTCTACTACT 
GCTTCTAGTT CTTCTCCCCC TTCTTCTGTC TCTCCCACTT CACTGACTCA AGGTGTGAAG 
AGCATCCGCA CAATCCATAC ACCTAGCTTC ACCAGCTACA AGTCACAGAA CGGAAGTGCA 
AGCAAAGGCC TGAATGCCAA GGAGACCTCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kiaa1217 ( XM_012965649.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821103.1 Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.

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CloneID	OXa36497
Clone ID Related Accession (Same CDS sequence)	XM_012965649.3 , XM_012965649.2
Accession Version	XM_012965649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sickle tail protein homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGGTTCC 
AGTGGAGGAG CACAAACCAG CAGAATGCCA ATCCCAATGT CTTCTAAAAG TAGGCAAGCA 
AACGTGGACA AAAGTAGCAA ACAGCCCAAA CTGCAGGAGC CGCAGCGCCA ATACAGACAG 
GCTAACGGAG GTACTAAGAA AGCTGGTGGG GATGTTAAGG CTTCTGCCCC TTCTTTATCT 
GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG 
TCTAGCGAGA CCTCTAACCC CCCTAATCCC TCTGTCAAGT CACATGTTGC CAATTCTAAC 
CCCCAAGCTA GCCGCACCGC TAACCCTTCA GCCCTTGTAC GTCCTGCTCA CAATGGCTCA 
TCCAAACTTC AGCCCCCGCC TACATATAAA GGTCACCATC TTTCATTCTC ACCGCAGGCC 
TCAAATGGCA GACCAGCTCT TCCCTCTACT ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT 
GTCTCTCCCA CTTCACTGAC TCAAGGTGTG AAGAGCATCC GCACAATCCA TACACCTAGC 
TTCACCAGCT ACAAGTCACA GAACGGAAGT GCAAGCAAAG GCCTGAATGC CAAGGAGACC 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821103.1
CDS	287..4552
Translation	MSKPSRLARPLPSHVPSKPPMNRKEDFSNRGRKSSLGEKILRAGSEGNLTRQQGQAAQIASRQAGSSSSTKGQSKHIQGSSLENIEFHQSKENLTNGNSHIPQKSGRNIPRRHTVGGPRSSKEILGMQTSEMDRKREAFLEHLKYKYPHHATAIMGHQERLRDQTKSPKLSSSPQPSVGDPAEHLSEASADSGDMMFEADSAVPFVRGSRTRASLPVVRSTNQTKERSLGTLFLQYGDDTKKMQMPNEITSVDTIRALFVSAFPQQLTMKILESPSTAIYIKDEGRNVYYELSDVRNIQDRCFLKVYNKDPAHAFNHTRGAVNGDVRMQRDISYSVREGPAIHRPGSATYPSHAGPVSPPPTPVPHSMPSSPSRIPYGGGRPAVVQSNTMQRERIPSVPAPRSISPSPSAILERRDVKPDEDMGNKNVQLLRNDSIYQDPYLYHEGRMSMASSHGGHPLDVPDHIIAYHRGSLRSASTYSNSSMQMEMMEQSMYRQKSRKHVESHLPTLGSKTPPTSPHRVADIRMMEMHAHNAHLPPHGMQADRSSPLRQSFKKEQGPGMFVEAKVRNTGGIMGMADIVPSPTDKQVFGGYGVAVPPKDPYTRERMHAMEKQIASLTGLVQTALLKGPSPNNSAPSDRVLKSANGSASSSDRPGNQGVAAAKNSLAISETITVINQQSSTCSQEMHVSLQDMRRNVAELRLQLHKMRQLQLQNQETVRLMMKKAEMEISSKVTEMVKGLEDPVQRQRVLVEQERQKYLGDEEQIVAKLCDLEDFVESLKKDQSTSQRSITLKDVEDRAFMLRQIGEAVSNLKGEFPYLQNKMRAVLRVEVEAVRFLKEEPHKLDSLLKRIRNMTDALSALRRHATDGSSKSIDNNQTSQVLITERVTETDSLQVHEEKSQVYLQPTQTDGGAAVESPTTSVKSEVVPLSAGLRVHQVQSSPVLMQQSQHSSALVNHTPAPSTQSSHSTELLVSHQTASVPPSVTPESARRNPPTAAQEGSSKQSLFIEEMSQVTYRNRAVSIEEAERKFEEKRQNLDHYNGKEFEKMLEEAQASIMKAIPSLEVPAQSQKVEIVDKKEVTEVKTIMEQDNDKVTKSPPPPPPRRMYPPGSGLNSVWTAESTFLVRKDSSKDSEEALPKVPSKAETEEPHLQSVEATVKSSAVKDEDEEEEGARIMAELQAFQKCSFVDVNPKSPNELSKADSQDYRATAVTQAKEKKGSSGGAQTSRMPIPMSSKSRQANVDKSSKQPKLQEPQRQYRQANGGTKKAGGDVKASAPSLSVSKIPGLSSNTGKSGSVPAQSSETSNPPNPSVKSHVANSNPQASRTANPSALVRPAHNGSSKLQPPPTYKGHHLSFSPQASNGRPALPSTTASSSSPPSSVSPTSLTQGVKSIRTIHTPSFTSYKSQNGSASKGLNAKETS

Target ORF information:

RefSeq Version	XM_012965649.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis KIAA1217 (kiaa1217), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965649.2

ORF Insert Sequence:

ATGTCCAAGC CATCACGTTT GGCCAGGCCC TTGCCGTCTC ATGTACCCTC TAAGCCGCCT 
ATGAATAGGA AAGAAGATTT TAGCAACCGA GGAAGGAAAA GCAGCTTAGG AGAGAAGATT 
CTGCGAGCAG GCAGCGAAGG GAACCTGACT AGGCAGCAAG GGCAGGCAGC CCAAATAGCC 
AGCAGGCAAG CCGGCTCCAG CAGCAGCACC AAAGGCCAAT CAAAACATAT TCAAGGCTCA 
TCCCTGGAAA ATATAGAATT TCATCAGTCA AAAGAGAATC TTACTAATGG GAACAGTCAC 
ATTCCACAAA AATCTGGGCG GAATATTCCA AGGAGACATA CTGTAGGGGG CCCTCGAAGT 
TCTAAAGAGA TTTTAGGAAT GCAGACATCG GAAATGGACA GGAAAAGAGA GGCTTTTCTA 
GAACACCTCA AATACAAATA TCCTCATCAT GCGACTGCAA TTATGGGCCA CCAGGAACGG 
CTGAGAGATC AGACAAAAAG TCCGAAGCTG TCTTCAAGCC CACAGCCGAG CGTAGGAGAC 
CCGGCAGAAC ATCTGTCAGA GGCGTCTGCT GATTCCGGAG ACATGATGTT CGAAGCGGAT 
TCGGCAGTGC CGTTTGTCCG AGGCAGTCGC ACAAGAGCCA GCCTTCCGGT GGTCAGATCC 
ACAAATCAAA CGAAAGAGAG ATCACTTGGT ACGCTCTTCC TACAGTATGG CGACGATACC 
AAGAAAATGC AGATGCCCAA TGAAATCACA AGCGTAGACA CGATCCGTGC CCTGTTCGTA 
AGTGCCTTCC CCCAGCAACT GACAATGAAG ATCCTGGAAT CGCCCAGCAC AGCCATTTAT 
ATTAAGGATG AGGGCCGAAA TGTCTATTAT GAGCTCAGCG ATGTCAGGAA CATACAAGAC 
AGATGCTTCC TCAAAGTCTA CAATAAAGAC CCTGCGCATG CATTTAACCA CACTCGTGGG 
GCGGTCAATG GAGATGTAAG GATGCAACGA GATATTTCCT ACTCGGTTCG GGAAGGCCCG 
GCGATCCATA GGCCTGGCTC TGCTACATAC CCCTCCCATG CTGGCCCAGT GTCACCCCCT 
CCCACACCTG TTCCCCACTC CATGCCATCA TCTCCATCAA GAATCCCCTA TGGCGGTGGG 
AGGCCTGCAG TGGTACAGAG CAACACCATG CAGAGGGAAA GAATACCAAG CGTGCCGGCA 
CCTAGATCCA TATCTCCTAG CCCCAGTGCT ATTTTGGAAA GGAGAGACGT AAAACCAGAT 
GAGGACATGG GCAATAAAAA TGTCCAGCTG CTAAGGAATG ACAGCATTTA CCAAGACCCT 
TATTTATACC ATGAAGGACG GATGAGTATG GCATCATCAC ATGGTGGGCA CCCTCTCGAC 
GTCCCTGATC ATATTATTGC ATACCACAGA GGGTCCTTGA GATCTGCAAG CACTTATTCT 
AATTCTTCTA TGCAAATGGA GATGATGGAA CAATCCATGT ACAGACAAAA AAGCCGTAAG 
CACGTAGAGA GTCACTTACC GACACTTGGT TCTAAAACTC CCCCAACCTC TCCTCACAGA 
GTGGCTGATA TAAGGATGAT GGAGATGCAT GCCCATAATG CCCATCTGCC TCCCCACGGC 
ATGCAGGCCG ACAGATCTTC TCCTCTCCGT CAGTCATTTA AAAAGGAGCA AGGGCCTGGC 
ATGTTTGTAG AAGCTAAAGT GCGAAACACA GGTGGAATAA TGGGCATGGC GGACATTGTC 
CCATCTCCTA CCGATAAGCA AGTATTTGGA GGGTATGGAG TAGCTGTGCC ACCAAAAGAC 
CCCTATACAA GAGAGAGGAT GCATGCCATG GAGAAACAGA TTGCCAGTTT AACTGGTCTT 
GTTCAGACTG CACTTTTGAA AGGGCCCAGC CCAAACAACT CTGCACCTAG CGACAGAGTC 
TTAAAATCAG CCAACGGCAG CGCTTCAAGC TCAGATCGGC CAGGAAATCA AGGTGTTGCT 
GCTGCAAAGA ATTCATTGGC CATTTCAGAA ACAATAACTG TTATCAACCA GCAGTCGTCT 
ACCTGCTCGC AGGAAATGCA CGTCAGCCTA CAGGACATGA GGCGCAATGT GGCCGAACTT 
CGTCTTCAGC TGCATAAGAT GAGACAACTG CAGCTGCAGA ACCAGGAGAC GGTGAGGCTG 
ATGATGAAGA AAGCGGAGAT GGAAATCAGC AGCAAAGTGA CTGAGATGGT CAAGGGCCTG 
GAGGACCCGG TGCAGAGACA GCGCGTCTTG GTGGAACAAG AGCGCCAGAA GTACCTCGGT 
GACGAGGAAC AAATTGTTGC AAAGTTGTGT GACCTTGAAG ACTTTGTGGA ATCCCTTAAA 
AAGGATCAGA GCACATCCCA GAGGTCGATC ACCCTAAAGG ACGTTGAGGA TCGGGCCTTC 
ATGCTGCGCC AGATAGGAGA GGCTGTTTCC AACCTGAAAG GAGAGTTTCC GTACCTCCAG 
AATAAGATGC GGGCCGTCTT GCGTGTGGAG GTGGAGGCCG TCAGATTTCT GAAAGAGGAG 
CCCCACAAAC TGGACAGCTT ACTGAAAAGG ATCCGAAACA TGACAGATGC CCTTTCTGCC 
TTAAGAAGAC ACGCCACTGA CGGATCCTCC AAATCCATTG ACAACAATCA GACCAGCCAG 
GTTCTAATAA CAGAAAGAGT AACTGAGACT GATTCACTCC AGGTCCATGA GGAAAAGTCC 
CAAGTGTACC TTCAGCCAAC ACAAACTGAT GGAGGAGCAG CAGTGGAATC GCCGACCACA 
TCTGTTAAAT CGGAAGTGGT TCCCCTGTCG GCCGGCCTCA GAGTCCATCA AGTTCAAAGT 
TCTCCGGTTC TCATGCAGCA GTCCCAGCAC TCGTCTGCAC TCGTGAACCA CACTCCTGCC 
CCCAGTACCC AGAGCAGTCA CAGCACAGAA TTACTAGTGA GCCATCAAAC TGCCAGCGTG 
CCACCCAGCG TCACCCCAGA AAGTGCAAGG AGGAACCCAC CGACGGCCGC ACAGGAAGGA 
TCTTCCAAGC AAAGTCTTTT TATTGAGGAA ATGAGTCAAG TGACTTACAG GAACCGAGCG 
GTGTCCATTG AGGAGGCAGA GAGAAAATTT GAAGAGAAGA GACAGAATCT TGATCATTAT 
AACGGAAAGG AATTTGAGAA AATGCTGGAA GAAGCACAAG CCAGTATCAT GAAGGCCATT 
CCCAGCTTAG AGGTGCCAGC CCAGTCCCAG AAAGTGGAGA TTGTGGACAA AAAAGAGGTC 
ACGGAGGTAA AAACCATAAT GGAGCAGGAT AACGATAAAG TAACAAAGTC TCCCCCTCCA 
CCACCACCTC GTCGTATGTA CCCACCAGGA TCTGGACTGA ACAGTGTATG GACAGCAGAA 
TCAACGTTCT TGGTCAGGAA GGACAGCAGC AAGGATTCGG AGGAAGCCCT TCCTAAGGTG 
CCATCAAAGG CAGAAACTGA AGAGCCTCAT CTTCAGTCGG TGGAGGCTAC AGTCAAGTCT 
TCTGCCGTAA AGGATGAGGA TGAAGAAGAG GAAGGAGCAA GGATTATGGC TGAACTTCAG 
GCCTTCCAGA AGTGTTCTTT TGTGGATGTA AATCCTAAAA GCCCAAATGA GCTTTCTAAA 
GCAGATAGCC AAGATTACAG AGCTACAGCT GTAACCCAAG CTAAAGAGAA GAAGGGTTCC 
AGTGGAGGAG CACAAACCAG CAGAATGCCA ATCCCAATGT CTTCTAAAAG TAGGCAAGCA 
AACGTGGACA AAAGTAGCAA ACAGCCCAAA CTGCAGGAGC CGCAGCGCCA ATACAGACAG 
GCTAACGGAG GTACTAAGAA AGCTGGTGGG GATGTTAAGG CTTCTGCCCC TTCTTTATCT 
GTTTCTAAGA TTCCAGGCCT TTCCTCTAAC ACTGGGAAAA GTGGCTCTGT GCCTGCTCAG 
TCTAGCGAGA CCTCTAACCC CCCTAATCCC TCTGTCAAGT CACATGTTGC CAATTCTAAC 
CCCCAAGCTA GCCGCACCGC TAACCCTTCA GCCCTTGTAC GTCCTGCTCA CAATGGCTCA 
TCCAAACTTC AGCCCCCGCC TACATATAAA GGTCACCATC TTTCATTCTC ACCGCAGGCC 
TCAAATGGCA GACCAGCTCT TCCCTCTACT ACTGCTTCTA GTTCTTCTCC CCCTTCTTCT 
GTCTCTCCCA CTTCACTGAC TCAAGGTGTG AAGAGCATCC GCACAATCCA TACACCTAGC 
TTCACCAGCT ACAAGTCACA GAACGGAAGT GCAAGCAAAG GCCTGAATGC CAAGGAGACC 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kiaa1217
Entrez Gene ID	100496212
Full Name	KIAA1217
General protein information	Preferred Names KIAA1217 Names sickle tail protein homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	LOC705851	XP_001099259.1
Homo sapiens (human)	KIAA1217	NP_062536.2
Rattus norvegicus (Norway rat)	Etl4	XP_006254390.1
Gallus gallus (chicken)	KIAA1217	XP_004939283.1
Danio rerio (zebrafish)	si:ch211-285f17.1	XP_005162642.1
Mus musculus (house mouse)	Etl4	NP_001074475.1
Xenopus tropicalis (tropical clawed frog)	kiaa1217	XP_002933193.2
Pan troglodytes (chimpanzee)	KIAA1217	XP_003951649.1
Canis lupus familiaris (dog)	KIAA1217	XP_005617059.1
Bos taurus (cattle)	KIAA1217	XP_002692115.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kiaa1217 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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