map4k1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following map4k1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map4k1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa36311 XM_012968765.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa65385 XM_031891995.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa65386 XM_031891996.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa65387 XM_031891997.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa36311
    Clone ID Related Accession (Same CDS sequence) XM_012968765.1
    Accession Version XM_012968765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GGTAAGGCTA TCTTACAGCT CTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824219.1
    CDS90..2426
    Translation

    Target ORF information:

    RefSeq Version XM_012968765.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GGTAAGGCTA TCTTACAGCT CTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65385
    Clone ID Related Accession (Same CDS sequence) XM_031891995.1
    Accession Version XM_031891995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GCTGTTTTCA GAGACACGGT TCTGGTGTTC
    ATGGAGAAAG GTTTCCAAGT ATTAAACATA CAGTCTGCAG AGCTTATAGC TGACTGCCGA
    GATCGCAGTT TACGTGTGTT ATCATCTGAC AGCAGGATGG TTATTGTAGA AGCTCCACAG
    TCAAGTCATG GCAACACAAA AAATCTTTAT ATCCAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747855.1
    CDS82..2583
    Translation

    Target ORF information:

    RefSeq Version XM_031891995.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GCTGTTTTCA GAGACACGGT TCTGGTGTTC
    ATGGAGAAAG GTTTCCAAGT ATTAAACATA CAGTCTGCAG AGCTTATAGC TGACTGCCGA
    GATCGCAGTT TACGTGTGTT ATCATCTGAC AGCAGGATGG TTATTGTAGA AGCTCCACAG
    TCAAGTCATG GCAACACAAA AAATCTTTAT ATCCAGGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65386
    Clone ID Related Accession (Same CDS sequence) XM_031891996.1
    Accession Version XM_031891996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GCTGTTTTCA GAGACACGGT TCTGGTGTTC
    ATGGAGAAAG GTTTCCAAGT ATTAAACATA CAGTCTGCAG AGCTTATAGC TGACTGCCGA
    GATCGCAGTT TACGTGTGTT ATCATCTGAC AGGATGGTTA TTGTAGAAGC TCCACAGTCA
    AGTCATGGCA ACACAAAAAA TCTTTATATC CAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747856.1
    CDS82..2580
    Translation

    Target ORF information:

    RefSeq Version XM_031891996.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGAGAGCTCT
    TGTTCTGGCC CAATACAGGT GATACAAACA GGATCTGACA GTGTCTTGGT GCTAACTGAC
    AATACACTAA ACTATGTAAA TCTTCATGGA GAACCACTGC AGACTTCCTT ATTGACAGAG
    ATTCATTTTA AATTCTCAGT GAAGTCAGTA GCTGTTTTCA GAGACACGGT TCTGGTGTTC
    ATGGAGAAAG GTTTCCAAGT ATTAAACATA CAGTCTGCAG AGCTTATAGC TGACTGCCGA
    GATCGCAGTT TACGTGTGTT ATCATCTGAC AGGATGGTTA TTGTAGAAGC TCCACAGTCA
    AGTCATGGCA ACACAAAAAA TCTTTATATC CAGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65387
    Clone ID Related Accession (Same CDS sequence) XM_031891997.1
    Accession Version XM_031891997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGGTGAGAGC
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747857.1
    CDS83..2248
    Translation

    Target ORF information:

    RefSeq Version XM_031891997.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase kinase 1 (map4k1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031891997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGATGTCC AGGGCCGTTT AACTTTAGAT ATATCGGACA GGGATCCTCA GGATGATTTT 
    GAGCTAATCC AGAGACTTGG CAGTGGAAGC TACGGTGAAG TTTTTAAGGC CAGGAATAAA
    GCTACTGGAG AACTGGCAGC TGTGAAAATT GTTACAAAAG AACCTGATGA AGATTTCTCT
    GTAATCCAGC AAGAGGTGCT GATCGTAAAG AGCTGTTCTC AAAAAAACAT TGTGGCATAT
    TATGGAAGTT ACATGCAAGA AAACAAGCTA TGGATTTGTA TGGAATACTG TGGAGGTGGA
    TCACTACAGG ACATTTACCA AGTGACAGGA GCCCTCTCAG AACTACAGAC GGCCTATGTT
    TGCAGGGAGA CCCTGCAGGG TCTGTCTTAT CTGCACAATC AAGGGAAGAT TCACAGAGAT
    ATCAAGGGTG CTAACATCCT TCTGAATGAC AATGGAGATG TTAAAGTAGC GGATTTTGGG
    ATTTCGGCTC AGCTCACTGC AACTTTTGCA AGGAGAAAGT CATTTATTGG GACACCTTAC
    TGGATGGCAC CAGAGGTGGC TGCTGTAGAG CTGAAAGGGG GCTACACAGA ACTGTGCGAT
    GTCTGGTCTC TGGGGATCAC TGCAATTGAA CTGGCTGAGC TTCAGCCCCC AATGTTCCAC
    ATACATCCCC TAAGAGTTCT CTTCCTAATG TCCAAGAGTG GATTTCAGCC TCCCAAACTG
    AAAGAGAAGT CCAAATGGTC AGCATCCTTC CACAATTTTG TAAAAGCTGC ATTAACGAAA
    AGTCCAAAAA AGAGACCAAG TGCTTCAAAA CTGCTATCTC ATCAGTTTGT GGCTCAACCA
    ACACTCAATG CTTCCCTCAC TCAAGACTTA CTGGAAAAAC TGAGAAATCC AGAAAAACAC
    AAAGATATTC CTAACCCAGA TGAAGAAGAA GAGGCAGAGG CATTGCCCAC TTTCTCGCGC
    ATCCTTTCGA AAAATCGCCA CCCTAAAGCC ACCCGATCCT CTTCAGACAT TCAGTTGCAC
    CAGATCAATT TAAAGCAGAT GGCAATAAGT AGCGAAGGAG ATCCTGCATT AATATTGGAT
    AAATTTCCTG GCATGGATTA CGGCCTAAGG GTGAGACCGA TCAGCCCAGC CTCCACAAGG
    AGCTTTTCAG ATGAAGATGA TGACTATGAT GATGTTGACA TCCCTCAAAA TGTTGATCAA
    AGCAACGATA CATCACCCAC AGGGGATTCC CCTCCTCCAC TGCCCCCCAA GCCAAGATTC
    CGCACTTGTT CAGCAGACGC TAAAAGAGGC CAGCAGAGAC TGCATGCACC TGCCAATCTG
    GTACGCTGCT GTAGTGGCCC CAACATTCCA AGGAACCCAG CACATGCCTG GTCTGAGGGA
    ATAGGGAATC CACTGCTTGC TGCCAGTTCT GAGCCATCCC TTTTATCATT TGATCCCTCA
    TCAGCTGTGC CCCCAACTCT ACCCCCTAAA AAGGGAAAGA AACAGACAGG GGTTATCTTC
    AAAAAGATTT TTAATGGATG CCCTCTTAAA ATTCACTCTG CAACATGCTG GAGTCACCCT
    ACTACAAAAG ACATGCATGT CATTTTAGGA GCTGAGGAAG GAATATTTAC TCTTAACCTC
    ATGGAGTCAC AAGCTTCTCT AGAGCTGCTC TATCCTGCTC GAACAACTTG GGTTTATTCC
    ATCAACAATG TTCTCATGAC TATAACAGGT AAAGCCTGTC ACCTGTACTC ACACACTCTC
    CTTGCCCTTC ACGAGCAGTC TCGGAGAGAG CACCGCCTGG TTCAGATTCC TGCACACAGA
    CTATTGCCAA GGAGGAAGCT CACATCAAGC AAAGTGCCAG ACACTAAGGG CTGCAAGAGT
    TGCTGCGTGG TTCACAATAC TATGCGTGAT AGCTGCTTTC TGTGTGTAGC TCTAGAGGCC
    AGTATCCTTC TCCTGGAATG GTATGAACCA CTTCAAAAAT TCATGCTGCT TAAGCACTTT
    GATTTTCCTT TGCCCAACCC ACTCAGGGTT TTCCAGATGT TGGTTGTACC TGGCGAGCAG
    TATCCATTGG TGTGTATTGG AGTCAGCAAG AGGTCACCAC CCTGTACTAG TGTTCGATTC
    CAGACAATCA ACCTTAACTC ACTGACCTCC TGGTTTACAG ATTGCGTGGC CGGTGAGAGC
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.