slc12a3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a3
Entrez Gene ID	100494755
Full Name	solute carrier family 12 member 3
Synonyms	ncc,ncct,tsc
General protein information	Preferred Names solute carrier family 12 member 3 Names solute carrier family 12 member 3 solute carrier family 12 (sodium/chloride transporters), member 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937171.4	XP_002937217.2	solute carrier family 12 member 3
XM_002937171.5	XP_002937217.2	solute carrier family 12 member 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology

The following slc12a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa56824	XM_002937171.5 Latest version!	Xenopus tropicalis solute carrier family 12 member 3 (slc12a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa08755	XM_002937171.4 Latest version!	Xenopus tropicalis solute carrier family 12 (sodium/chloride transporters), member 3 (slc12a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a3 ( XM_002937171.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937217.2 Xenopus tropicalis solute carrier family 12 member 3 (slc12a3), mRNA.

Price & Availability↑

CloneID	OXa56824
Clone ID Related Accession (Same CDS sequence)	XM_002937171.5
Accession Version	XM_002937171.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937171.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGA ATAGGAATGA TAATCCAGTG GAGAATGCTC CCTCCAATGG AAGGTTCACC 
ATCCGCTCCC TGATCAGCAC TGAGGACAGC ATCAAAGCTC AATATGGGCA ACAAGAATCA 
GCATATGATA CATTTAACCC CAATAATCTT ACACAGACCA GCACCCTTTA CATGAGAACA 
TTTGGCTACA ATACTGTGGA TGCAATTCCA AATTATGACC ATTATGCCAA CACTGAAATC 
TCTGGACAAA TAAAGAAGGT CAGACCTACA CTGGAAGATC TTCATTCCAT CCTAAAGCAG 
GATTCAAGTC ATCATCTGGA CCCTCCTGAT TATGACAACA CAGTGGAACC TCCTGATGGT 
GAGGCAGAAA ACACTGAGCA GAAGCCAGCT GGAGAACCTG TCCGGTTTGG ATGGATTAAA 
GGGGTGATGA TCCGCTGCAT GCTGAATATT TGGGGAGTTA TCCTGTATCT CCGGCTGCCA 
TGGATAACAG CACAAGCAGG AATAGCACTA ACCTGGCTCA TCATCTTTAT GTCTGTGACT 
GTAACAAGCA TCACAGGACT CTCCATTTCA GCTATTTCCA CCAATGGCAA AGTCAAGTCA 
GGTGGGACCT ATTTTCTCAT CTCAAGAAGC CTTGGCCCAG AGTTAGGAGG ATCAATTGGC 
CTTATATTTG CCTTTGCAAA TGCTGTGGCA GTAGCGATGC ACACAGTGGG ATTTGCTGAA 
ACTGTCCGAG ATCTGTTGAG GGATTATGAC TCGACTATTG TTGACCCAAT AAATGACATC 
AGGATCATTG GGATAATAAC GGTTATAGTT CTTCTTGGAA TTTCACTTGC TGGCATGGAA 
TGGGAGGCAA AGGCTCAGAT ACTTTTCTTC ATTGTGATCA TGATCTCATT TGCAAACTAC 
TTGGTTGGCA CAGTGATACC CGCCACAGAA GAGAAACAGG CCAAGGGTTT TTTCAGTTAT 
CGAGCTGATA TTTTTGCTGA AAATTTTGTG CCTAAATGGC GTGGACAAGA TGGAAGTTTC 
TTTGGAATGT TTTCTATTTT CTTCCCATCA GCCACTGGAA TTCTTGCTGG AGCCAATATT 
TCTGGGGATC TTAAGGACCC AACTGTTGCT ATTCCTAAAG GAACGCTCAT GTCAATATTC 
TGGACCACTG TATCCTATCT GGTCATATCA GCCACTATAG GATCCTGTGT TGTTCGTGAT 
GCTTCTGGCA GTCTTAATGA CACTATAACA GATGGGGGCT TAAACTGTGT TGGACTTGCC 
TGCGGGTATG GGTGGAACTT CACTGACTGT GCAACAAAAC AGAACTGTAA ATATGGGCTG 
ATCAATCAGT ACCAGTCCAT GAGCATGGTG TCTGGATTTG CGCCACTTGT GACTGCTGGA 
ATCTTTGGAG CAACACTGTC ATCTGCTCTG GCTTGCCTTG TGTCTGCTCC CAAAGTGTTT 
CAGTGTCTCT GTAAGGACAA CCTTTATCCA CTCATTGGAT TCTTTGGGAA AGGCTATGGA 
AAGAACAATG AGCCAATTCG TGGATATCTA CTAACTTTCC TCATTGCATC TGCCTTCATC 
TTGATTGGGG AACTGAACAC CATTGCTCCC ATCATTTCTA ATTTCTTTCT GTGCTCCTAC 
GCTCTGATTA ATTTTAGCTG TTTCCACGCA TCGATCACAA ACTCTCCAGG CTGGAGGCCA 
TCATTTAAAT ATTACAGTAA GTGGGCATCT CTCTTTGGAG CTGTAGCATC TGTTGTGATC 
ATGTTCCTGC TGGCCTGGGA ATCAGCTCTA ATTGCTATTG GCATTGTAAT TTTCCTTCTT 
GGCTATGTCT TGTACAAAAA GCCCGAAGCA AACTGGGGCT CATCAGTACA AGCAGGATCT 
TATAACATGG CCTTGTCATA CTGCGTGGGG CTCAACCAAG TAGAAGAACA CATAAAAAAT 
TTTAGGCCAC AGTGTCTGGT TTTGACAGGT CCTCCAAACT TCCGTCCTGC CCTTGTGGAT 
TTTGTGGGAA CCTTTACTAA AAAACAAAGC CTGATGATCT GTGGGAATGT GATTATTGGT 
CCACGTAAAC AGAAACTCCA GGAGATAAGC CCAGAAGGAC ACATCAAGTG GCTGAACAAG 
AGGAAGATAA AAGCTTTTTA CACTGGACTG ATTGCAGATG ACTTGCGGAG TGGAACGCAG 
ATGCTTATTC AGGCAGCAGG TCTTGGACAT ATGAGGCCAA ATGTCCTCGT CATGGGATTT 
AAAAAAAACT GGTCAACATC TCACCCAAGA AGCACTGAGA ATTATGTGGG TATACTGCAT 
GATGCACTTG ACTTTAAATG TGGTCTGTGT CTGCTAAGGA TGAAAGAAGG TATAAATATA 
TCTCGTGTCA TGCAAGCTCA CATTAACCCC GTATATGAAC AAACAGAAGA ACCAAAACTG 
AAAGATGGGG GAGAGAATGG AGCCAGCTGT TCAGCACAAT CAACATTGGA TCCAGAAGCT 
GTCACAGTTG AACAACAAGC AAGCACCATC TTCCAGATTA AACAGGATAA AAAGACAATT 
GATATCTATT GGCTTTTTGA TGATGGAGGA CTGACACTTC TGATCCCCTA TTTGCTCAGC 
CGTAAAAAGA GATGGCAGAA AGCTAAAATC AGAGTGTTTG TAGGTGGACA GATCAACAGA 
ATGGATGAAG AGTGCAAAGC GATGATATCC CTATTAAGTA AATTTAGAAT TGGATTTCAT 
GAAGTTCACG TTCTTCCTGA TATCAACCAA AAGCCTCGGC CTGAGCACAT CAAGCGATTT 
GAGGATCTAA TTGCTCCATA TATGCTAAAC GACGGTTTCA AAGATGAGCT GACAGTAAAT 
GAAATGAGGC GTGACTGTCC TTGGAAGATC TCAGATGAGG AGATAAAAAA GAACAGAGCA 
AAGTCCCTAA GACAAATACG TCTAAATGAA GTGCTTCTGG ATTATTCCCG AGATGCGGGG 
TTGATTGTCA TAACAATGCC AATTGCCAGA AAAGGAAAGT GTCCCAGCAC CCTATACATG 
GCCTGGCTGG AAACTCTTTC ACAAGACCTC AGACCTCCTG TTCTGCTGAC CCGAGGGAAC 
CAACAGAATG TGCTGACCAT GTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937217.2
CDS	13..3105
Translation	MAENRNDNPVENAPSNGRFTIRSLISTEDSIKAQYGQQESAYDTFNPNNLTQTSTLYMRTFGYNTVDAIPNYDHYANTEISGQIKKVRPTLEDLHSILKQDSSHHLDPPDYDNTVEPPDGEAENTEQKPAGEPVRFGWIKGVMIRCMLNIWGVILYLRLPWITAQAGIALTWLIIFMSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVRDLLRDYDSTIVDPINDIRIIGIITVIVLLGISLAGMEWEAKAQILFFIVIMISFANYLVGTVIPATEEKQAKGFFSYRADIFAENFVPKWRGQDGSFFGMFSIFFPSATGILAGANISGDLKDPTVAIPKGTLMSIFWTTVSYLVISATIGSCVVRDASGSLNDTITDGGLNCVGLACGYGWNFTDCATKQNCKYGLINQYQSMSMVSGFAPLVTAGIFGATLSSALACLVSAPKVFQCLCKDNLYPLIGFFGKGYGKNNEPIRGYLLTFLIASAFILIGELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFKYYSKWASLFGAVASVVIMFLLAWESALIAIGIVIFLLGYVLYKKPEANWGSSVQAGSYNMALSYCVGLNQVEEHIKNFRPQCLVLTGPPNFRPALVDFVGTFTKKQSLMICGNVIIGPRKQKLQEISPEGHIKWLNKRKIKAFYTGLIADDLRSGTQMLIQAAGLGHMRPNVLVMGFKKNWSTSHPRSTENYVGILHDALDFKCGLCLLRMKEGINISRVMQAHINPVYEQTEEPKLKDGGENGASCSAQSTLDPEAVTVEQQASTIFQIKQDKKTIDIYWLFDDGGLTLLIPYLLSRKKRWQKAKIRVFVGGQINRMDEECKAMISLLSKFRIGFHEVHVLPDINQKPRPEHIKRFEDLIAPYMLNDGFKDELTVNEMRRDCPWKISDEEIKKNRAKSLRQIRLNEVLLDYSRDAGLIVITMPIARKGKCPSTLYMAWLETLSQDLRPPVLLTRGNQQNVLTMYCQ

Target ORF information:

RefSeq Version	XM_002937171.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 member 3 (slc12a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937171.5

ORF Insert Sequence:

ATGGCTGAGA ATAGGAATGA TAATCCAGTG GAGAATGCTC CCTCCAATGG AAGGTTCACC 
ATCCGCTCCC TGATCAGCAC TGAGGACAGC ATCAAAGCTC AATATGGGCA ACAAGAATCA 
GCATATGATA CATTTAACCC CAATAATCTT ACACAGACCA GCACCCTTTA CATGAGAACA 
TTTGGCTACA ATACTGTGGA TGCAATTCCA AATTATGACC ATTATGCCAA CACTGAAATC 
TCTGGACAAA TAAAGAAGGT CAGACCTACA CTGGAAGATC TTCATTCCAT CCTAAAGCAG 
GATTCAAGTC ATCATCTGGA CCCTCCTGAT TATGACAACA CAGTGGAACC TCCTGATGGT 
GAGGCAGAAA ACACTGAGCA GAAGCCAGCT GGAGAACCTG TCCGGTTTGG ATGGATTAAA 
GGGGTGATGA TCCGCTGCAT GCTGAATATT TGGGGAGTTA TCCTGTATCT CCGGCTGCCA 
TGGATAACAG CACAAGCAGG AATAGCACTA ACCTGGCTCA TCATCTTTAT GTCTGTGACT 
GTAACAAGCA TCACAGGACT CTCCATTTCA GCTATTTCCA CCAATGGCAA AGTCAAGTCA 
GGTGGGACCT ATTTTCTCAT CTCAAGAAGC CTTGGCCCAG AGTTAGGAGG ATCAATTGGC 
CTTATATTTG CCTTTGCAAA TGCTGTGGCA GTAGCGATGC ACACAGTGGG ATTTGCTGAA 
ACTGTCCGAG ATCTGTTGAG GGATTATGAC TCGACTATTG TTGACCCAAT AAATGACATC 
AGGATCATTG GGATAATAAC GGTTATAGTT CTTCTTGGAA TTTCACTTGC TGGCATGGAA 
TGGGAGGCAA AGGCTCAGAT ACTTTTCTTC ATTGTGATCA TGATCTCATT TGCAAACTAC 
TTGGTTGGCA CAGTGATACC CGCCACAGAA GAGAAACAGG CCAAGGGTTT TTTCAGTTAT 
CGAGCTGATA TTTTTGCTGA AAATTTTGTG CCTAAATGGC GTGGACAAGA TGGAAGTTTC 
TTTGGAATGT TTTCTATTTT CTTCCCATCA GCCACTGGAA TTCTTGCTGG AGCCAATATT 
TCTGGGGATC TTAAGGACCC AACTGTTGCT ATTCCTAAAG GAACGCTCAT GTCAATATTC 
TGGACCACTG TATCCTATCT GGTCATATCA GCCACTATAG GATCCTGTGT TGTTCGTGAT 
GCTTCTGGCA GTCTTAATGA CACTATAACA GATGGGGGCT TAAACTGTGT TGGACTTGCC 
TGCGGGTATG GGTGGAACTT CACTGACTGT GCAACAAAAC AGAACTGTAA ATATGGGCTG 
ATCAATCAGT ACCAGTCCAT GAGCATGGTG TCTGGATTTG CGCCACTTGT GACTGCTGGA 
ATCTTTGGAG CAACACTGTC ATCTGCTCTG GCTTGCCTTG TGTCTGCTCC CAAAGTGTTT 
CAGTGTCTCT GTAAGGACAA CCTTTATCCA CTCATTGGAT TCTTTGGGAA AGGCTATGGA 
AAGAACAATG AGCCAATTCG TGGATATCTA CTAACTTTCC TCATTGCATC TGCCTTCATC 
TTGATTGGGG AACTGAACAC CATTGCTCCC ATCATTTCTA ATTTCTTTCT GTGCTCCTAC 
GCTCTGATTA ATTTTAGCTG TTTCCACGCA TCGATCACAA ACTCTCCAGG CTGGAGGCCA 
TCATTTAAAT ATTACAGTAA GTGGGCATCT CTCTTTGGAG CTGTAGCATC TGTTGTGATC 
ATGTTCCTGC TGGCCTGGGA ATCAGCTCTA ATTGCTATTG GCATTGTAAT TTTCCTTCTT 
GGCTATGTCT TGTACAAAAA GCCCGAAGCA AACTGGGGCT CATCAGTACA AGCAGGATCT 
TATAACATGG CCTTGTCATA CTGCGTGGGG CTCAACCAAG TAGAAGAACA CATAAAAAAT 
TTTAGGCCAC AGTGTCTGGT TTTGACAGGT CCTCCAAACT TCCGTCCTGC CCTTGTGGAT 
TTTGTGGGAA CCTTTACTAA AAAACAAAGC CTGATGATCT GTGGGAATGT GATTATTGGT 
CCACGTAAAC AGAAACTCCA GGAGATAAGC CCAGAAGGAC ACATCAAGTG GCTGAACAAG 
AGGAAGATAA AAGCTTTTTA CACTGGACTG ATTGCAGATG ACTTGCGGAG TGGAACGCAG 
ATGCTTATTC AGGCAGCAGG TCTTGGACAT ATGAGGCCAA ATGTCCTCGT CATGGGATTT 
AAAAAAAACT GGTCAACATC TCACCCAAGA AGCACTGAGA ATTATGTGGG TATACTGCAT 
GATGCACTTG ACTTTAAATG TGGTCTGTGT CTGCTAAGGA TGAAAGAAGG TATAAATATA 
TCTCGTGTCA TGCAAGCTCA CATTAACCCC GTATATGAAC AAACAGAAGA ACCAAAACTG 
AAAGATGGGG GAGAGAATGG AGCCAGCTGT TCAGCACAAT CAACATTGGA TCCAGAAGCT 
GTCACAGTTG AACAACAAGC AAGCACCATC TTCCAGATTA AACAGGATAA AAAGACAATT 
GATATCTATT GGCTTTTTGA TGATGGAGGA CTGACACTTC TGATCCCCTA TTTGCTCAGC 
CGTAAAAAGA GATGGCAGAA AGCTAAAATC AGAGTGTTTG TAGGTGGACA GATCAACAGA 
ATGGATGAAG AGTGCAAAGC GATGATATCC CTATTAAGTA AATTTAGAAT TGGATTTCAT 
GAAGTTCACG TTCTTCCTGA TATCAACCAA AAGCCTCGGC CTGAGCACAT CAAGCGATTT 
GAGGATCTAA TTGCTCCATA TATGCTAAAC GACGGTTTCA AAGATGAGCT GACAGTAAAT 
GAAATGAGGC GTGACTGTCC TTGGAAGATC TCAGATGAGG AGATAAAAAA GAACAGAGCA 
AAGTCCCTAA GACAAATACG TCTAAATGAA GTGCTTCTGG ATTATTCCCG AGATGCGGGG 
TTGATTGTCA TAACAATGCC AATTGCCAGA AAAGGAAAGT GTCCCAGCAC CCTATACATG 
GCCTGGCTGG AAACTCTTTC ACAAGACCTC AGACCTCCTG TTCTGCTGAC CCGAGGGAAC 
CAACAGAATG TGCTGACCAT GTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a3 ( XM_002937171.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937217.2 Xenopus tropicalis solute carrier family 12 (sodium/chloride transporters), member 3 (slc12a3), mRNA.

Price & Availability↑

CloneID	OXa08755
Clone ID Related Accession (Same CDS sequence)	XM_002937171.4
Accession Version	XM_002937171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGA ATAGGAATGA TAATCCAGTG GAGAATGCTC CCTCCAATGG AAGGTTCACC 
ATCCGCTCCC TGATCAGCAC TGAGGACAGC ATCAAAGCTC AATATGGGCA ACAAGAATCA 
GCATATGATA CATTTAACCC CAATAATCTT ACACAGACCA GCACCCTTTA CATGAGAACA 
TTTGGCTACA ATACTGTGGA TGCAATTCCA AATTATGACC ATTATGCCAA CACTGAAATC 
TCTGGACAAA TAAAGAAGGT CAGACCTACA CTGGAAGATC TTCATTCCAT CCTAAAGCAG 
GATTCAAGTC ATCATCTGGA CCCTCCTGAT TATGACAACA CAGTGGAACC TCCTGATGGT 
GAGGCAGAAA ACACTGAGCA GAAGCCAGCT GGAGAACCTG TCCGGTTTGG ATGGATTAAA 
GGGGTGATGA TCCGCTGCAT GCTGAATATT TGGGGAGTTA TCCTGTATCT CCGGCTGCCA 
TGGATAACAG CACAAGCAGG AATAGCACTA ACCTGGCTCA TCATCTTTAT GTCTGTGACT 
GTAACAAGCA TCACAGGACT CTCCATTTCA GCTATTTCCA CCAATGGCAA AGTCAAGTCA 
GGTGGGACCT ATTTTCTCAT CTCAAGAAGC CTTGGCCCAG AGTTAGGAGG ATCAATTGGC 
CTTATATTTG CCTTTGCAAA TGCTGTGGCA GTAGCGATGC ACACAGTGGG ATTTGCTGAA 
ACTGTCCGAG ATCTGTTGAG GGATTATGAC TCGACTATTG TTGACCCAAT AAATGACATC 
AGGATCATTG GGATAATAAC GGTTATAGTT CTTCTTGGAA TTTCACTTGC TGGCATGGAA 
TGGGAGGCAA AGGCTCAGAT ACTTTTCTTC ATTGTGATCA TGATCTCATT TGCAAACTAC 
TTGGTTGGCA CAGTGATACC CGCCACAGAA GAGAAACAGG CCAAGGGTTT TTTCAGTTAT 
CGAGCTGATA TTTTTGCTGA AAATTTTGTG CCTAAATGGC GTGGACAAGA TGGAAGTTTC 
TTTGGAATGT TTTCTATTTT CTTCCCATCA GCCACTGGAA TTCTTGCTGG AGCCAATATT 
TCTGGGGATC TTAAGGACCC AACTGTTGCT ATTCCTAAAG GAACGCTCAT GTCAATATTC 
TGGACCACTG TATCCTATCT GGTCATATCA GCCACTATAG GATCCTGTGT TGTTCGTGAT 
GCTTCTGGCA GTCTTAATGA CACTATAACA GATGGGGGCT TAAACTGTGT TGGACTTGCC 
TGCGGGTATG GGTGGAACTT CACTGACTGT GCAACAAAAC AGAACTGTAA ATATGGGCTG 
ATCAATCAGT ACCAGTCCAT GAGCATGGTG TCTGGATTTG CGCCACTTGT GACTGCTGGA 
ATCTTTGGAG CAACACTGTC ATCTGCTCTG GCTTGCCTTG TGTCTGCTCC CAAAGTGTTT 
CAGTGTCTCT GTAAGGACAA CCTTTATCCA CTCATTGGAT TCTTTGGGAA AGGCTATGGA 
AAGAACAATG AGCCAATTCG TGGATATCTA CTAACTTTCC TCATTGCATC TGCCTTCATC 
TTGATTGGGG AACTGAACAC CATTGCTCCC ATCATTTCTA ATTTCTTTCT GTGCTCCTAC 
GCTCTGATTA ATTTTAGCTG TTTCCACGCA TCGATCACAA ACTCTCCAGG CTGGAGGCCA 
TCATTTAAAT ATTACAGTAA GTGGGCATCT CTCTTTGGAG CTGTAGCATC TGTTGTGATC 
ATGTTCCTGC TGGCCTGGGA ATCAGCTCTA ATTGCTATTG GCATTGTAAT TTTCCTTCTT 
GGCTATGTCT TGTACAAAAA GCCAGAAGCA AACTGGGGCT CATCAGTACA AGCAGGATCT 
TATAACATGG CCTTGTCATA CTGCGTGGGG CTCAACCAAG TAGAAGAACA CATAAAAAAT 
TTTAGGCCAC AGTGTCTGGT TTTGACAGGT CCTCCAAACT TCCGTCCTGC CCTTGTGGAT 
TTTGTGGGAA CCTTTACTAA AAAACAAAGC CTGATGATCT GTGGGAATGT GATTATTGGT 
CCACGTAAAC AGAAACTCCA GGAGATAAGC CCAGAAGGAC ACATCAAGTG GCTGAACAAG 
AGGAAGATAA AAGCTTTTTA CACTGGACTG ATTGCAGATG ACTTGCGGAG TGGAACGCAG 
ATGCTTATTC AGGCAGCAGG TCTTGGACAT ATGAGGCCAA ATGTCCTCGT CATGGGATTT 
AAAAAAAACT GGTCAACATC TCACCCAAGA AGCACTGAGA ATTATGTGGG TATACTGCAT 
GATGCACTTG ACTTTAAATG TGGTCTGTGT CTGCTAAGGA TGAAAGAAGG TATAAATATA 
TCTCGTGTCA TGCAAGCTCA CATTAACCCC GTATATGAAC AAACAGAAGA ACCAAAACTG 
AAAGATGGGG GAGAGAATGG AGCCAGCTGT TCAGCACAAT CAACATTGGA TCCAGAAGCT 
GTCACAGTTG AACAACAAGC AAGCACCATC TTCCAGATTA AACAGGATAA AAAGACAATT 
GATATCTATT GGCTTTTTGA TGATGGAGGA CTGACACTTC TGATCCCCTA TTTGCTCAGC 
CGTAAAAAGA GATGGCAGAA AGCTAAAATC AGAGTGTTTG TAGGTGGACA GATCAACAGA 
ATGGATGAAG AGTGCAAAGC GATGATATCC CTATTAAGTA AATTTAGAAT TGGATTTCAT 
GAAGTTCACG TTCTTCCTGA TATCAACCAA AAGCCTCGGC CTGAGCACAT CAAGCGATTT 
GAGGATCTAA TTGCTCCATA TATGCTAAAC GACGGTTTCA AAGATGAGCT GACAGTAAAT 
GAAATGAGGC GTGACTGTCC TTGGAAGATC TCAGATGAGG AGATAAAAAA GAACAGAGCA 
AAGTCCCTAA GACAAATACG TCTAAATGAA GTGCTTCTGG ATTATTCCCG AGATGCGGGG 
TTGATTGTCA TAACAATGCC AATTGCCAGA AAAGGAAAGT GTCCCAGCAC CCTATACATG 
GCCTGGCTGG AAACTCTTTC ACAAGACCTC AGACCTCCTG TTCTGCTGAC CCGAGGGAAC 
CAACAGAATG TGCTGACCAT GTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937217.2
CDS	35..3127
Translation	MAENRNDNPVENAPSNGRFTIRSLISTEDSIKAQYGQQESAYDTFNPNNLTQTSTLYMRTFGYNTVDAIPNYDHYANTEISGQIKKVRPTLEDLHSILKQDSSHHLDPPDYDNTVEPPDGEAENTEQKPAGEPVRFGWIKGVMIRCMLNIWGVILYLRLPWITAQAGIALTWLIIFMSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVRDLLRDYDSTIVDPINDIRIIGIITVIVLLGISLAGMEWEAKAQILFFIVIMISFANYLVGTVIPATEEKQAKGFFSYRADIFAENFVPKWRGQDGSFFGMFSIFFPSATGILAGANISGDLKDPTVAIPKGTLMSIFWTTVSYLVISATIGSCVVRDASGSLNDTITDGGLNCVGLACGYGWNFTDCATKQNCKYGLINQYQSMSMVSGFAPLVTAGIFGATLSSALACLVSAPKVFQCLCKDNLYPLIGFFGKGYGKNNEPIRGYLLTFLIASAFILIGELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFKYYSKWASLFGAVASVVIMFLLAWESALIAIGIVIFLLGYVLYKKPEANWGSSVQAGSYNMALSYCVGLNQVEEHIKNFRPQCLVLTGPPNFRPALVDFVGTFTKKQSLMICGNVIIGPRKQKLQEISPEGHIKWLNKRKIKAFYTGLIADDLRSGTQMLIQAAGLGHMRPNVLVMGFKKNWSTSHPRSTENYVGILHDALDFKCGLCLLRMKEGINISRVMQAHINPVYEQTEEPKLKDGGENGASCSAQSTLDPEAVTVEQQASTIFQIKQDKKTIDIYWLFDDGGLTLLIPYLLSRKKRWQKAKIRVFVGGQINRMDEECKAMISLLSKFRIGFHEVHVLPDINQKPRPEHIKRFEDLIAPYMLNDGFKDELTVNEMRRDCPWKISDEEIKKNRAKSLRQIRLNEVLLDYSRDAGLIVITMPIARKGKCPSTLYMAWLETLSQDLRPPVLLTRGNQQNVLTMYCQ

Target ORF information:

RefSeq Version	XM_002937171.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (sodium/chloride transporters), member 3 (slc12a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937171.4

ORF Insert Sequence:

ATGGCTGAGA ATAGGAATGA TAATCCAGTG GAGAATGCTC CCTCCAATGG AAGGTTCACC 
ATCCGCTCCC TGATCAGCAC TGAGGACAGC ATCAAAGCTC AATATGGGCA ACAAGAATCA 
GCATATGATA CATTTAACCC CAATAATCTT ACACAGACCA GCACCCTTTA CATGAGAACA 
TTTGGCTACA ATACTGTGGA TGCAATTCCA AATTATGACC ATTATGCCAA CACTGAAATC 
TCTGGACAAA TAAAGAAGGT CAGACCTACA CTGGAAGATC TTCATTCCAT CCTAAAGCAG 
GATTCAAGTC ATCATCTGGA CCCTCCTGAT TATGACAACA CAGTGGAACC TCCTGATGGT 
GAGGCAGAAA ACACTGAGCA GAAGCCAGCT GGAGAACCTG TCCGGTTTGG ATGGATTAAA 
GGGGTGATGA TCCGCTGCAT GCTGAATATT TGGGGAGTTA TCCTGTATCT CCGGCTGCCA 
TGGATAACAG CACAAGCAGG AATAGCACTA ACCTGGCTCA TCATCTTTAT GTCTGTGACT 
GTAACAAGCA TCACAGGACT CTCCATTTCA GCTATTTCCA CCAATGGCAA AGTCAAGTCA 
GGTGGGACCT ATTTTCTCAT CTCAAGAAGC CTTGGCCCAG AGTTAGGAGG ATCAATTGGC 
CTTATATTTG CCTTTGCAAA TGCTGTGGCA GTAGCGATGC ACACAGTGGG ATTTGCTGAA 
ACTGTCCGAG ATCTGTTGAG GGATTATGAC TCGACTATTG TTGACCCAAT AAATGACATC 
AGGATCATTG GGATAATAAC GGTTATAGTT CTTCTTGGAA TTTCACTTGC TGGCATGGAA 
TGGGAGGCAA AGGCTCAGAT ACTTTTCTTC ATTGTGATCA TGATCTCATT TGCAAACTAC 
TTGGTTGGCA CAGTGATACC CGCCACAGAA GAGAAACAGG CCAAGGGTTT TTTCAGTTAT 
CGAGCTGATA TTTTTGCTGA AAATTTTGTG CCTAAATGGC GTGGACAAGA TGGAAGTTTC 
TTTGGAATGT TTTCTATTTT CTTCCCATCA GCCACTGGAA TTCTTGCTGG AGCCAATATT 
TCTGGGGATC TTAAGGACCC AACTGTTGCT ATTCCTAAAG GAACGCTCAT GTCAATATTC 
TGGACCACTG TATCCTATCT GGTCATATCA GCCACTATAG GATCCTGTGT TGTTCGTGAT 
GCTTCTGGCA GTCTTAATGA CACTATAACA GATGGGGGCT TAAACTGTGT TGGACTTGCC 
TGCGGGTATG GGTGGAACTT CACTGACTGT GCAACAAAAC AGAACTGTAA ATATGGGCTG 
ATCAATCAGT ACCAGTCCAT GAGCATGGTG TCTGGATTTG CGCCACTTGT GACTGCTGGA 
ATCTTTGGAG CAACACTGTC ATCTGCTCTG GCTTGCCTTG TGTCTGCTCC CAAAGTGTTT 
CAGTGTCTCT GTAAGGACAA CCTTTATCCA CTCATTGGAT TCTTTGGGAA AGGCTATGGA 
AAGAACAATG AGCCAATTCG TGGATATCTA CTAACTTTCC TCATTGCATC TGCCTTCATC 
TTGATTGGGG AACTGAACAC CATTGCTCCC ATCATTTCTA ATTTCTTTCT GTGCTCCTAC 
GCTCTGATTA ATTTTAGCTG TTTCCACGCA TCGATCACAA ACTCTCCAGG CTGGAGGCCA 
TCATTTAAAT ATTACAGTAA GTGGGCATCT CTCTTTGGAG CTGTAGCATC TGTTGTGATC 
ATGTTCCTGC TGGCCTGGGA ATCAGCTCTA ATTGCTATTG GCATTGTAAT TTTCCTTCTT 
GGCTATGTCT TGTACAAAAA GCCAGAAGCA AACTGGGGCT CATCAGTACA AGCAGGATCT 
TATAACATGG CCTTGTCATA CTGCGTGGGG CTCAACCAAG TAGAAGAACA CATAAAAAAT 
TTTAGGCCAC AGTGTCTGGT TTTGACAGGT CCTCCAAACT TCCGTCCTGC CCTTGTGGAT 
TTTGTGGGAA CCTTTACTAA AAAACAAAGC CTGATGATCT GTGGGAATGT GATTATTGGT 
CCACGTAAAC AGAAACTCCA GGAGATAAGC CCAGAAGGAC ACATCAAGTG GCTGAACAAG 
AGGAAGATAA AAGCTTTTTA CACTGGACTG ATTGCAGATG ACTTGCGGAG TGGAACGCAG 
ATGCTTATTC AGGCAGCAGG TCTTGGACAT ATGAGGCCAA ATGTCCTCGT CATGGGATTT 
AAAAAAAACT GGTCAACATC TCACCCAAGA AGCACTGAGA ATTATGTGGG TATACTGCAT 
GATGCACTTG ACTTTAAATG TGGTCTGTGT CTGCTAAGGA TGAAAGAAGG TATAAATATA 
TCTCGTGTCA TGCAAGCTCA CATTAACCCC GTATATGAAC AAACAGAAGA ACCAAAACTG 
AAAGATGGGG GAGAGAATGG AGCCAGCTGT TCAGCACAAT CAACATTGGA TCCAGAAGCT 
GTCACAGTTG AACAACAAGC AAGCACCATC TTCCAGATTA AACAGGATAA AAAGACAATT 
GATATCTATT GGCTTTTTGA TGATGGAGGA CTGACACTTC TGATCCCCTA TTTGCTCAGC 
CGTAAAAAGA GATGGCAGAA AGCTAAAATC AGAGTGTTTG TAGGTGGACA GATCAACAGA 
ATGGATGAAG AGTGCAAAGC GATGATATCC CTATTAAGTA AATTTAGAAT TGGATTTCAT 
GAAGTTCACG TTCTTCCTGA TATCAACCAA AAGCCTCGGC CTGAGCACAT CAAGCGATTT 
GAGGATCTAA TTGCTCCATA TATGCTAAAC GACGGTTTCA AAGATGAGCT GACAGTAAAT 
GAAATGAGGC GTGACTGTCC TTGGAAGATC TCAGATGAGG AGATAAAAAA GAACAGAGCA 
AAGTCCCTAA GACAAATACG TCTAAATGAA GTGCTTCTGG ATTATTCCCG AGATGCGGGG 
TTGATTGTCA TAACAATGCC AATTGCCAGA AAAGGAAAGT GTCCCAGCAC CCTATACATG 
GCCTGGCTGG AAACTCTTTC ACAAGACCTC AGACCTCCTG TTCTGCTGAC CCGAGGGAAC 
CAACAGAATG TGCTGACCAT GTACTGCCAG TAA