nos2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nos2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42693 XM_018091491.1
Latest version!
Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa52170 XM_002935296.5
Latest version!
Xenopus tropicalis nitric oxide synthase 2 (nos2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa05725 XM_002935296.4
Latest version!
Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42693
    Clone ID Related Accession (Same CDS sequence) XM_018091491.1
    Accession Version XM_018091491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, inducible isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA TGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946980.1
    CDS310..3666
    Translation

    Target ORF information:

    RefSeq Version XM_018091491.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA TGCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52170
    Clone ID Related Accession (Same CDS sequence) XM_002935296.5
    Accession Version XM_002935296.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, inducible
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935296.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TAAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCCAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA
    TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT
    TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935342.2
    CDS275..3757
    Translation

    Target ORF information:

    RefSeq Version XM_002935296.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 2 (nos2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935296.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TAAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCCAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA
    TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT
    TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05725
    Clone ID Related Accession (Same CDS sequence) XM_002935296.4
    Accession Version XM_002935296.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, inducible isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA
    TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT
    TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935342.2
    CDS310..3792
    Translation

    Target ORF information:

    RefSeq Version XM_002935296.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935296.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
    AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC
    ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA
    TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA
    TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA
    GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG
    GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA
    ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT
    AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA
    GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA
    ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG
    TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT
    ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA
    CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC
    ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG
    ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA
    GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC
    CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG
    TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT
    TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA
    CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG
    TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT
    ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG
    CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC
    ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT
    GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA
    TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT
    GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC
    GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA
    AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG
    AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT
    TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA
    AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT
    GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA
    TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC
    TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT
    CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG
    CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG
    GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA
    GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG
    GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT
    TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA
    AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC
    AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA
    CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT
    CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC
    CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT
    GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG
    AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA
    GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG
    AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT
    GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC
    ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG
    AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC
    GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG
    CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA
    TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT
    TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.