npc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following npc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa59950 XM_004915212.4
Latest version!
Xenopus tropicalis NPC intracellular cholesterol transporter 1 (npc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa11603 XM_004915212.3
Latest version!
Xenopus tropicalis Niemann-Pick disease, type C1 (npc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa59950
    Clone ID Related Accession (Same CDS sequence) XM_004915212.4
    Accession Version XM_004915212.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product NPC intracellular cholesterol transporter 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915212.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCACCT GGAAGCACCA GTCCCTGATA CCGCTCTTCT TCCTAACAAC CCTGTGGCTT 
    CCTGGGCGGG TATCTTCCCA GCACTGTGTC TGGTATGGGG AGTGTGGCAA TTCAACATTA
    GAAGACAAAC GGTTAAACTG TCGATACTCG GGGCCTCCTA TCTCCTTGCC TGAGGAAGGA
    CATACACTGC TACAGCAACT TTGCCCATCA CTTTATTATG GAAATAACAC AGTTTGCTGT
    GATGTCCAGC AACTCAACAC ACTTAACAGC AATCTCCAGC TTCCATTGCA GTTTCTTTCA
    AGATGCCCTT CTTGTTTTTA TAATTTCATG ACCATGTTCT GTGAGCTGAC CTGCAGTCCC
    CATCAGTCAG AGTTTCTAAA TGTTACCCAG ACTGAACCAT TTGTTGGGCC TAGTCCTCAA
    AGGGCAAATG AAAGCATTGT GGCCATGGAG TATTACATAG GAGAACAATT TGCTAATGCG
    ATGTACAATG CATGCAAGGA TGTAGAAGCC CCCTCGAGTA ATGTCAAGGC TTTGGCTTTG
    CTGTGTGGAA AGGATGTCAA GGACTGTAAT GCTACTAACT GGATTCAGTA CATGTTCAGC
    AAAGATAATG GGCAGGTTCC TTTTACCATC ACACCTATCT TTTCAGATGA TCCTGTGACT
    TCCATGCTTC CCATGAACAA CGCCACCAAG GGCTGCGATG AGCCTGTGGA AGAAGGCGGG
    GCTTCCTGTA GTTGTCAGGA CTGCGCTCTT TCTTGTGGTC CTGTGCCCCA GCCTCCACCA
    CTTCCTCCTC CATGGCTTAT ATTTGGCTTG GATGCTATGG CTGTCATCAT GTGGATAGTT
    TATGTTGTGT TCTTACTACT CTTTTCTGCA GTCATTATTG GATCGTGGTG TTACAGGAAA
    AAGAATATTG TCTCAGAATA TGCTCCTATT GATAGCACTT TAGCATATTC TGTCAATTCC
    ACTCCAATAG CAGGAGAGCC TTCGTGCTGT GACCGGCTTG GTGAAAGACT CGAGAACTTT
    CTTCGAATTG GATTTACAAA GTGGGGTTCT ATTTGCGTCC GGAAACCATG GATTGTTATC
    TTTTTCTCTG TGCTTTTAAT TGTGATATGT TGCTTTGGCC TCAAATACAT GAGGATTACT
    ACTGATCCTG TTGAACTCTG GTCAGATCCT CACAGCCAGG CTCGGCAAGA AAAGGAATAT
    TTTGACTCTC ACTTTGGACC CTTCTTTCGC ACTGAACAAC TAATCATCAC TGCCCCCAAC
    AGTTCAGTCC ACAATTACTC ACCATATCCT TCTGGCAGTG ATGTGCCTTT TGGCCCACCT
    CTAGACTTTA ATATTCTTGC TCAAGTCTTG GAGTTACAAA ATGGCATTGA AAACCTAAAA
    GCTTATTACA ATAATGAGAC TGTAATGTTA AAAGACATCT GTTTGGCTCC ATTGGCCCCA
    TACAACAACA ACTGCACAAT TTTCAGCGTT CTCAACTATT TCCAGAACAG CTTCTCTGTG
    CTGAATCATT CCATACAGGA TGATTTCTAT GTTTATGCTG ATTACCACAC TCACTTCTTA
    TACTGTGTGC GATCTCCAGC ATCTTTGAAT GACACATCAG TACTCCATGA TCCCTGCCTG
    GGAACATTTG GTGGCCCCAT ATTTCCATGG CTGGTTCTTG GAGGATATGA TGATGAAAAT
    TATAATAATG CCACCGCCCT GGTGATTACT TTTCCAGTAA ATAATTTCCG CAACGACACA
    GAAAAATTGA ATAGAGCAAA GGCTTGGGAA TTAGAGTTTA TCAAATTTGT GGAAAATTAT
    AGCAATCCCA ATCTGTCCAT TTCATTCTCT GCCGAGAGAA GCATTGAAGA TGAGATAAAC
    CGTGAAAGTA AAAGTGATGT TACTACTGTT GTCATCAGTT ATGCCTTAAT GTTTGTCTAC
    ATATCCTTGG CTTTGGGTCA GATTGGCCAC TGCAGTAGTA TCTTGGTGGA CTCTAAGATC
    TCGCTGGGTA TTGCTGGTAT TCTGATTGTG CTCAGTTCAG TAGCCTGCTC TCTGGGTATC
    TTCAGCTATG CTGGCGTGTC ACTCACTCTT ATTGTCATTG AGGTGATCCC ATTTCTGGTG
    CTAGCTGTTG GAGTTGACAA CATATACATT ATTGTGCAGA GATATCAGAG AGATGAGCGT
    CTTCATGATG AGACTCTGGA CCAGCAGATT GGCAGAATAC TGGGAGATGT GGCGCCGAGT
    ATATTTCTGT CAGCTTTTGC CGAAACGGTT GCCTTTTTTT TGGGAGCATT GTCCTCTATG
    CCAGCCGTGC GGACATTTTC ACTCTTTGCT GGGATGGCCG TGTTTCTAGA CTTCCTCCTT
    CAGATCACCT GCTTCATCAG CCTTCTTAGT CTGGACATCA GGCGACAAGA GAAAAACCGC
    TTAGATATCC TTTGCTGTGT GCCTGGATGC AAGAGAAACC GTGGCACTGA CAAACCGAAG
    AGCTGGCTGT TTTTGTTCTT CAAAAAGCTT TATGCCCCTG TACTGATGAA GGATTGGATA
    AGACCTATTG TGGTATCCGT TTTTGTTGGA ATCCTGTCCT TTAGCATTGC GGTTGTCAAC
    AAAGTAGAAA TAGGTCTTGA TCAGTCTCTC TCCATGCCTG ATGATTCCTA CATGCTAGAT
    TATTTTGGCT CACTCAGCAC ATACCTCCAT ACTGGCCCCC CAGTGTATTT TGTAGTTGAG
    GAAGGACATA ATTATACCAC AAAGGAAGGA CAGGACAAGG TGTGTGGTGG AGCTGGATGC
    AATAACAATT CATTGGTGCA AGAGATCTAC ACCGCTGCTG GGATAAGCAA CTATACAAGG
    ATCGGATACG CACCTTCCTC TTGGATTGAC GATTACTTTG ATTGGGTGAA GCCCCAGTCT
    ACTTGCTGTC GAATATATAA TAACACAGAT CAATTCTGTA ATGCTTCAGT GGTTAACGCA
    TCCTGCTTAT CATGCCGGTC CTACACTCCA GAAGGAAAGC GCAAACCAGT TGGAGAGGAT
    TTTATGCATT TCCTGCCCAT GTTTCTCTCT GATAATCCAA ACCCCAAATG TGGGAAAGGG
    GGCCACGCGG CATATGGCTC TGCAGTTGAT CTCCTTGACA GTAACACTAA TGTTGGTGCA
    ACTTATTTTA TGACCTATCA TACAATTCTG AAGAACTCCA CAGACTTCAT TGACGCCATG
    AGGAAAGCCC GTACCATCGC ACAGAATATC ACTGATAATA TGGACATCCC AGGGAAGAAA
    TACAAAGTAT TCCCTTACAG TATTTTCTAT GTATTTTATG AGCAGTATTT AACCATTGTG
    GATGACACCA TCTTCAACTT GTGCGTGTCT CTGGCAGCAG TATTCTTTGT GACAGTGATC
    CTTCTGGGCT TTGAGCTCTG GTCAGCTGTC CTTATGTGCA TCACGATTTC TATGATCCTT
    GTTAACATGT TTGGAGTCAT GTGGCTCTGG GGCATCAGCT TAAATGCAGT GTCCCTTGTC
    AATTTAGTCA TGAGCTGTGG CATTTCAGTA GAGTTCTGCA GCCACGTGAC CAGAGCCTTT
    TCAGTTAGCA CAAGGGGAAA CCGGGTACAA CGTGCTGAGG ATGCCCTGGC TCATATGGGT
    AGCTCGGTGT TCAGTGGTAT AACACTCACA AAGTTTGTTG GTATCGGTGT TCTTGGCTTG
    TCCAAATCTC AGATATTCAA GATCTTCTAC TTCAGAATGT ATCTGGCTAT GGTGCTTCTT
    GGGGCAGCAC ATGGCTTAAT ATTTCTGCCT GTTCTACTTA GTTACTTAGG CCCCCAAGTG
    AATAAAGCAA AGGTGCAAGC TGCGCTGCAG AGAGCCCGAG GCACAGAGCA AGAGCGTCTT
    TTTAACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915269.2
    CDS382..4233
    Translation

    Target ORF information:

    RefSeq Version XM_004915212.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NPC intracellular cholesterol transporter 1 (npc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915212.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCACCT GGAAGCACCA GTCCCTGATA CCGCTCTTCT TCCTAACAAC CCTGTGGCTT 
    CCTGGGCGGG TATCTTCCCA GCACTGTGTC TGGTATGGGG AGTGTGGCAA TTCAACATTA
    GAAGACAAAC GGTTAAACTG TCGATACTCG GGGCCTCCTA TCTCCTTGCC TGAGGAAGGA
    CATACACTGC TACAGCAACT TTGCCCATCA CTTTATTATG GAAATAACAC AGTTTGCTGT
    GATGTCCAGC AACTCAACAC ACTTAACAGC AATCTCCAGC TTCCATTGCA GTTTCTTTCA
    AGATGCCCTT CTTGTTTTTA TAATTTCATG ACCATGTTCT GTGAGCTGAC CTGCAGTCCC
    CATCAGTCAG AGTTTCTAAA TGTTACCCAG ACTGAACCAT TTGTTGGGCC TAGTCCTCAA
    AGGGCAAATG AAAGCATTGT GGCCATGGAG TATTACATAG GAGAACAATT TGCTAATGCG
    ATGTACAATG CATGCAAGGA TGTAGAAGCC CCCTCGAGTA ATGTCAAGGC TTTGGCTTTG
    CTGTGTGGAA AGGATGTCAA GGACTGTAAT GCTACTAACT GGATTCAGTA CATGTTCAGC
    AAAGATAATG GGCAGGTTCC TTTTACCATC ACACCTATCT TTTCAGATGA TCCTGTGACT
    TCCATGCTTC CCATGAACAA CGCCACCAAG GGCTGCGATG AGCCTGTGGA AGAAGGCGGG
    GCTTCCTGTA GTTGTCAGGA CTGCGCTCTT TCTTGTGGTC CTGTGCCCCA GCCTCCACCA
    CTTCCTCCTC CATGGCTTAT ATTTGGCTTG GATGCTATGG CTGTCATCAT GTGGATAGTT
    TATGTTGTGT TCTTACTACT CTTTTCTGCA GTCATTATTG GATCGTGGTG TTACAGGAAA
    AAGAATATTG TCTCAGAATA TGCTCCTATT GATAGCACTT TAGCATATTC TGTCAATTCC
    ACTCCAATAG CAGGAGAGCC TTCGTGCTGT GACCGGCTTG GTGAAAGACT CGAGAACTTT
    CTTCGAATTG GATTTACAAA GTGGGGTTCT ATTTGCGTCC GGAAACCATG GATTGTTATC
    TTTTTCTCTG TGCTTTTAAT TGTGATATGT TGCTTTGGCC TCAAATACAT GAGGATTACT
    ACTGATCCTG TTGAACTCTG GTCAGATCCT CACAGCCAGG CTCGGCAAGA AAAGGAATAT
    TTTGACTCTC ACTTTGGACC CTTCTTTCGC ACTGAACAAC TAATCATCAC TGCCCCCAAC
    AGTTCAGTCC ACAATTACTC ACCATATCCT TCTGGCAGTG ATGTGCCTTT TGGCCCACCT
    CTAGACTTTA ATATTCTTGC TCAAGTCTTG GAGTTACAAA ATGGCATTGA AAACCTAAAA
    GCTTATTACA ATAATGAGAC TGTAATGTTA AAAGACATCT GTTTGGCTCC ATTGGCCCCA
    TACAACAACA ACTGCACAAT TTTCAGCGTT CTCAACTATT TCCAGAACAG CTTCTCTGTG
    CTGAATCATT CCATACAGGA TGATTTCTAT GTTTATGCTG ATTACCACAC TCACTTCTTA
    TACTGTGTGC GATCTCCAGC ATCTTTGAAT GACACATCAG TACTCCATGA TCCCTGCCTG
    GGAACATTTG GTGGCCCCAT ATTTCCATGG CTGGTTCTTG GAGGATATGA TGATGAAAAT
    TATAATAATG CCACCGCCCT GGTGATTACT TTTCCAGTAA ATAATTTCCG CAACGACACA
    GAAAAATTGA ATAGAGCAAA GGCTTGGGAA TTAGAGTTTA TCAAATTTGT GGAAAATTAT
    AGCAATCCCA ATCTGTCCAT TTCATTCTCT GCCGAGAGAA GCATTGAAGA TGAGATAAAC
    CGTGAAAGTA AAAGTGATGT TACTACTGTT GTCATCAGTT ATGCCTTAAT GTTTGTCTAC
    ATATCCTTGG CTTTGGGTCA GATTGGCCAC TGCAGTAGTA TCTTGGTGGA CTCTAAGATC
    TCGCTGGGTA TTGCTGGTAT TCTGATTGTG CTCAGTTCAG TAGCCTGCTC TCTGGGTATC
    TTCAGCTATG CTGGCGTGTC ACTCACTCTT ATTGTCATTG AGGTGATCCC ATTTCTGGTG
    CTAGCTGTTG GAGTTGACAA CATATACATT ATTGTGCAGA GATATCAGAG AGATGAGCGT
    CTTCATGATG AGACTCTGGA CCAGCAGATT GGCAGAATAC TGGGAGATGT GGCGCCGAGT
    ATATTTCTGT CAGCTTTTGC CGAAACGGTT GCCTTTTTTT TGGGAGCATT GTCCTCTATG
    CCAGCCGTGC GGACATTTTC ACTCTTTGCT GGGATGGCCG TGTTTCTAGA CTTCCTCCTT
    CAGATCACCT GCTTCATCAG CCTTCTTAGT CTGGACATCA GGCGACAAGA GAAAAACCGC
    TTAGATATCC TTTGCTGTGT GCCTGGATGC AAGAGAAACC GTGGCACTGA CAAACCGAAG
    AGCTGGCTGT TTTTGTTCTT CAAAAAGCTT TATGCCCCTG TACTGATGAA GGATTGGATA
    AGACCTATTG TGGTATCCGT TTTTGTTGGA ATCCTGTCCT TTAGCATTGC GGTTGTCAAC
    AAAGTAGAAA TAGGTCTTGA TCAGTCTCTC TCCATGCCTG ATGATTCCTA CATGCTAGAT
    TATTTTGGCT CACTCAGCAC ATACCTCCAT ACTGGCCCCC CAGTGTATTT TGTAGTTGAG
    GAAGGACATA ATTATACCAC AAAGGAAGGA CAGGACAAGG TGTGTGGTGG AGCTGGATGC
    AATAACAATT CATTGGTGCA AGAGATCTAC ACCGCTGCTG GGATAAGCAA CTATACAAGG
    ATCGGATACG CACCTTCCTC TTGGATTGAC GATTACTTTG ATTGGGTGAA GCCCCAGTCT
    ACTTGCTGTC GAATATATAA TAACACAGAT CAATTCTGTA ATGCTTCAGT GGTTAACGCA
    TCCTGCTTAT CATGCCGGTC CTACACTCCA GAAGGAAAGC GCAAACCAGT TGGAGAGGAT
    TTTATGCATT TCCTGCCCAT GTTTCTCTCT GATAATCCAA ACCCCAAATG TGGGAAAGGG
    GGCCACGCGG CATATGGCTC TGCAGTTGAT CTCCTTGACA GTAACACTAA TGTTGGTGCA
    ACTTATTTTA TGACCTATCA TACAATTCTG AAGAACTCCA CAGACTTCAT TGACGCCATG
    AGGAAAGCCC GTACCATCGC ACAGAATATC ACTGATAATA TGGACATCCC AGGGAAGAAA
    TACAAAGTAT TCCCTTACAG TATTTTCTAT GTATTTTATG AGCAGTATTT AACCATTGTG
    GATGACACCA TCTTCAACTT GTGCGTGTCT CTGGCAGCAG TATTCTTTGT GACAGTGATC
    CTTCTGGGCT TTGAGCTCTG GTCAGCTGTC CTTATGTGCA TCACGATTTC TATGATCCTT
    GTTAACATGT TTGGAGTCAT GTGGCTCTGG GGCATCAGCT TAAATGCAGT GTCCCTTGTC
    AATTTAGTCA TGAGCTGTGG CATTTCAGTA GAGTTCTGCA GCCACGTGAC CAGAGCCTTT
    TCAGTTAGCA CAAGGGGAAA CCGGGTACAA CGTGCTGAGG ATGCCCTGGC TCATATGGGT
    AGCTCGGTGT TCAGTGGTAT AACACTCACA AAGTTTGTTG GTATCGGTGT TCTTGGCTTG
    TCCAAATCTC AGATATTCAA GATCTTCTAC TTCAGAATGT ATCTGGCTAT GGTGCTTCTT
    GGGGCAGCAC ATGGCTTAAT ATTTCTGCCT GTTCTACTTA GTTACTTAGG CCCCCAAGTG
    AATAAAGCAA AGGTGCAAGC TGCGCTGCAG AGAGCCCGAG GCACAGAGCA AGAGCGTCTT
    TTTAACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11603
    Clone ID Related Accession (Same CDS sequence) XM_004915212.3
    Accession Version XM_004915212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Niemann-Pick C1 protein
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCACCT GGAAGCACCA GTCCCTGATA CCGCTCTTCT TCCTAATAAC CCTGTGGCTT 
    CCTGGGCGGG TATCTTCCCA GCACTGTGTC TGGTATGGGG AGTGTGGCAA TTCAACATTA
    GAAGACAAAC GGTTAAACTG TCGATACTCG GGGCCTCCTA TCTCCTTGCC TGAGGAAGGA
    CATACACTGC TACAGCAACT TTGCCCATCA CTTTATTATG GAAATAACAC AGTTTGCTGT
    GATGTCCAGC AACTCAACAC ACTTAACAGC AATCTTCAGC TTCCATTGCA GTTTCTTTCA
    AGATGCCCTT CTTGTTTTTA TAATTTCATG ACCATGTTCT GTGAGCTGAC CTGCAGTCCC
    TATCAGTCAG AGTTTCTAAA TGTTACCCAG ACTGAACCAT TTGTTGGGCC TAGTCCTCAA
    AGGGCAAATG AAAGCATTGT GGCCATGGAG TATTACATAG GAGAACAATT TGCTAATGCG
    ATGTACAATG CATGCAAGGA TGTAGAAGCC CCCTCGAGTA ATGTCAAGGC TTTGGCTTTG
    CTGTGTGGAA AGGATGTCAA GGACTGTAAT GCTACTAACT GGATTCAGTA CATGTTCAGC
    AAAGATAATG GGCAGGTTCC TTTTACCATC ACACCTATCT TTTCAGATGA TCCTGTGACT
    TCCATGCTTC CCATGAACAA CGCCACCAAG GGCTGCGATG AGCCTGTGGA AGAAGGCGGG
    GCTTCCTGTA GTTGTCAGGA CTGCGCTCTT TCTTGTGGTC CTGTGCCCCA GCCTCCACCA
    CTTCCTCCTC CATGGCTTAT ATTTGGCTTG GATGCTATGG CTGTCATCAT GTGGATAGTT
    TATGTTGTGT TCTTACTACT CTTTTCTGCA GTCATTATTG GATCGTGGTG TTACAGGAAA
    AAGAATATTG TCTCAGAATA TGCTCCTATT GATAGCACTT TAGCATATTC TGTCAATTCC
    ACTCCAATAG CAGGAGAGCC TTCGTGCTGT GACCGGCTTG GTGAAAGACT CGAGAACTTT
    CTTCGAATTG GATTTACAAA GTGGGGTTCA TTTTGCGTCC GGAAACCATG GATTGTTATC
    TTTTTCTCTG TGCTTTTAAT TGTGATATGT TGCTTTGGCC TCAAATACAT GAGGATTACT
    ACTGATCCTG TTGAACTCTG GTCAGATCCT CACAGCCAGG CTCGGCAAGA AAAGGAATAT
    TTTGACTCTC ACTTTGGACC CTTCTTTCGC ACTGAACAAC TAATCATCAC TGCCCCCAAC
    AGTTCAGTCC ACAATTACTC ACCATATCCT TCTGGCAGTG ATGTGCCTTT TGGCCCACCT
    CTAGACTTTA ATATTCTTGC TCAAGTCTTG GAGTTACAAA ATGGCATTGA AAACCTAAAA
    GCTTATTACA ATAATGAGAC TGTAATGTTA AAAGACATCT GTTTGGCTCC ATTGGCCCCA
    TACAACAACA ACTGCACAAT TTTCAGCGTT CTCAACTATT TCCAGAACAG CTTCTCTGTG
    CTGAATCATT CCATACAGGA TGATTTCTAT GTTTATGCTG ATTACCACAC TCACTTCTTA
    TACTGTGTGC GATCTCCAGC ATCTTTGAAT GACACATCAG TACTCCATGA TCCCTGCCTG
    GGAACATTTG GTGGCCCCAT ATTTCCATGG CTGGTTCTTG GAGGATATGA TGATGAAAAT
    TATAATAATG CCACCGCCCT GGTGATTACT TTTCCAGTAA ATAATTTCCG CAACGACACA
    GAAAAATTGA ATAGAGCAAA GGCTTGGGAA TTAGAGTTTA TCAAATTTGT GGAAAATTAT
    AGCAATCCCA ATCTGTCCAT TTCATTCTCT GCCGAGAGAA GCATTGAAGA TGAGATAAAC
    CGTGAAAGTA AAAGTGATGT TACTACTGTT GTCATCAGTT ATGCCTTAAT GTTTGTCTAC
    ATATCCTTGG CTTTGGGTCA GATTGGCCAC TGCAGTAGTA TCTTGGTGGA CTCTAAGATC
    TCGCTGGGTA TTGCTGGTAT TCTGATTGTG CTCAGTTCAG TAGCCTGCTC TCTGGGTATC
    TTCAGCTATG CTGGCGTGTC ACTCACTCTT ATTGTCATTG AGGTGATCCC ATTTCTGGTG
    CTAGCTGTTG GAGTTGACAA CATATACATT ATTGTGCAGA GATATCAGAG AGATGAGCGT
    CTTCATGATG AGACTCTGGA CCAGCAGATT GGCAGAATAC TGGGAGATGT GGCGCCGAGT
    ATATTTCTGT CAGCTTTTGC CGAAACGGTT GCCTTTTTTT TGGGAGCATT GTCCTCTATG
    CCAGCCGTGC GGACATTTTC ACTCTTTGCT GGGATGGCCG TGTTTCTAGA CTTCCTCCTT
    CAGATCACCT GCTTCATCAG CCTTCTTAGT CTGGACATCA GGCGACAAGA GAAAAACCGC
    TTAGATATCC TTTGCTGTGT GCCTGGATGC AAGAGAAACC GTGGCACTGA CAAACCGAAG
    AGCTGGCTGT TTTTGTTCTT CAAAAAGCTT TATGCCCCTG TACTGATGAA GGATTGGATA
    AGACCTATTG TGGTATCCGT TTTTGTTGGA ATCCTGTCCT TTAGCATTGC GGTTGTCAAC
    AAAGTAGAAA TAGGTCTTGA TCAGTCTCTC TCCATGCCTG ATGATTCCTA CATGCTAGAT
    TATTTTGGCT CACTCAGCAC ATACCTCCAT ACTGGCCCCC CAGTGTATTT TGTAGTTGAG
    GAAGGACATA ATTATACCAC AAAGGAAGGA CAGGACAAGG TGTGTGGTGG AGCTGGATGC
    AATAACAATT CATTGGTGCA AGAGATCTAC ACCGCTGCTG GGATAAGCAA CTATACAAGG
    ATCGGATACG CACCTTCCTC TTGGATTGAC GATTACTTTG ATTGGGTGAA GCCCCAGTCT
    ACTTGCTGTC GAATATATAA TAACACAGAT CAATTCTGTA ATGCTTCAGT GGTTAACGCA
    TCCTGCTTAT CATGCCGGTC CTACACTCCA GAAGGAAAGC GCAAACCAGT TGGAGAGGAT
    TTTATGCATT TCCTGCCCAT GTTTCTCTCT GATAATCCAA ACCCCAAATG TGGGAAAGGG
    GGCCACGCGG CATATGGCTC TGCAGTTGAT CTCCTTGACA GTAACACTAA TGTTGGTGCA
    ACTTATTTTA TGACCTATCA TACAATTCTG AAGAACTCCA CAGACTTCAT TGACGCCATG
    AGGAAAGCCC GTACCATCGC ACAGAATATC ACTGATAATA TGGACATCCC AGGGAAGAAA
    TACAAAGTAT TCCCTTACAG TATTTTCTAT GTATTTTATG AGCAGTATTT AACCATTGTG
    GATGACACCA TCTTCAACTT GTGCGTGTCT CTGGCAGCAG TATTCTTTGT GACAGTGATC
    CTTCTGGGCT TTGAGCTCTG GTCAGCTGTC CTTATGTGCA TCACGATTTC TATGATCCTT
    GTTAACATGT TTGGAGTCAT GTGGCTCTGG GGCATCAGCT TAAATGCAGT GTCCCTTGTC
    AATTTAGTCA TGAGCTGTGG CATTTCAGTA GAGTTCTGCA GCCACGTGAC CAGAGCCTTT
    TCAGTTAGCA CAAGGGGAAA CCGGGTACAA CGTGCTGAGG ATGCCCTGGC TCATATGGGT
    AGCTCGGTGT TCAGTGGTAT AACACTCACA AAGTTTGTTG GTATCGGTGT TCTTGGCTTG
    TCCAAATCTC AGATATTCAA GATCTTCTAC TTCAGAATGT ATCTGGCTAT GGTGCTTCTT
    GGGGCAGCAC ATGGCTTAAT ATTTCTGCCT GTTCTACTTA GTTACTTAGG CCCCCAAGTG
    AATAAAGCAA AGGTGCAAGC TGCGCTGCAG AGAGCCCGAG GCACAGAGCA AGAGCGTCTT
    TTTAACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004915269.1
    CDS408..4259
    Translation

    Target ORF information:

    RefSeq Version XM_004915212.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Niemann-Pick disease, type C1 (npc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004915212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTCACCT GGAAGCACCA GTCCCTGATA CCGCTCTTCT TCCTAATAAC CCTGTGGCTT 
    CCTGGGCGGG TATCTTCCCA GCACTGTGTC TGGTATGGGG AGTGTGGCAA TTCAACATTA
    GAAGACAAAC GGTTAAACTG TCGATACTCG GGGCCTCCTA TCTCCTTGCC TGAGGAAGGA
    CATACACTGC TACAGCAACT TTGCCCATCA CTTTATTATG GAAATAACAC AGTTTGCTGT
    GATGTCCAGC AACTCAACAC ACTTAACAGC AATCTTCAGC TTCCATTGCA GTTTCTTTCA
    AGATGCCCTT CTTGTTTTTA TAATTTCATG ACCATGTTCT GTGAGCTGAC CTGCAGTCCC
    TATCAGTCAG AGTTTCTAAA TGTTACCCAG ACTGAACCAT TTGTTGGGCC TAGTCCTCAA
    AGGGCAAATG AAAGCATTGT GGCCATGGAG TATTACATAG GAGAACAATT TGCTAATGCG
    ATGTACAATG CATGCAAGGA TGTAGAAGCC CCCTCGAGTA ATGTCAAGGC TTTGGCTTTG
    CTGTGTGGAA AGGATGTCAA GGACTGTAAT GCTACTAACT GGATTCAGTA CATGTTCAGC
    AAAGATAATG GGCAGGTTCC TTTTACCATC ACACCTATCT TTTCAGATGA TCCTGTGACT
    TCCATGCTTC CCATGAACAA CGCCACCAAG GGCTGCGATG AGCCTGTGGA AGAAGGCGGG
    GCTTCCTGTA GTTGTCAGGA CTGCGCTCTT TCTTGTGGTC CTGTGCCCCA GCCTCCACCA
    CTTCCTCCTC CATGGCTTAT ATTTGGCTTG GATGCTATGG CTGTCATCAT GTGGATAGTT
    TATGTTGTGT TCTTACTACT CTTTTCTGCA GTCATTATTG GATCGTGGTG TTACAGGAAA
    AAGAATATTG TCTCAGAATA TGCTCCTATT GATAGCACTT TAGCATATTC TGTCAATTCC
    ACTCCAATAG CAGGAGAGCC TTCGTGCTGT GACCGGCTTG GTGAAAGACT CGAGAACTTT
    CTTCGAATTG GATTTACAAA GTGGGGTTCA TTTTGCGTCC GGAAACCATG GATTGTTATC
    TTTTTCTCTG TGCTTTTAAT TGTGATATGT TGCTTTGGCC TCAAATACAT GAGGATTACT
    ACTGATCCTG TTGAACTCTG GTCAGATCCT CACAGCCAGG CTCGGCAAGA AAAGGAATAT
    TTTGACTCTC ACTTTGGACC CTTCTTTCGC ACTGAACAAC TAATCATCAC TGCCCCCAAC
    AGTTCAGTCC ACAATTACTC ACCATATCCT TCTGGCAGTG ATGTGCCTTT TGGCCCACCT
    CTAGACTTTA ATATTCTTGC TCAAGTCTTG GAGTTACAAA ATGGCATTGA AAACCTAAAA
    GCTTATTACA ATAATGAGAC TGTAATGTTA AAAGACATCT GTTTGGCTCC ATTGGCCCCA
    TACAACAACA ACTGCACAAT TTTCAGCGTT CTCAACTATT TCCAGAACAG CTTCTCTGTG
    CTGAATCATT CCATACAGGA TGATTTCTAT GTTTATGCTG ATTACCACAC TCACTTCTTA
    TACTGTGTGC GATCTCCAGC ATCTTTGAAT GACACATCAG TACTCCATGA TCCCTGCCTG
    GGAACATTTG GTGGCCCCAT ATTTCCATGG CTGGTTCTTG GAGGATATGA TGATGAAAAT
    TATAATAATG CCACCGCCCT GGTGATTACT TTTCCAGTAA ATAATTTCCG CAACGACACA
    GAAAAATTGA ATAGAGCAAA GGCTTGGGAA TTAGAGTTTA TCAAATTTGT GGAAAATTAT
    AGCAATCCCA ATCTGTCCAT TTCATTCTCT GCCGAGAGAA GCATTGAAGA TGAGATAAAC
    CGTGAAAGTA AAAGTGATGT TACTACTGTT GTCATCAGTT ATGCCTTAAT GTTTGTCTAC
    ATATCCTTGG CTTTGGGTCA GATTGGCCAC TGCAGTAGTA TCTTGGTGGA CTCTAAGATC
    TCGCTGGGTA TTGCTGGTAT TCTGATTGTG CTCAGTTCAG TAGCCTGCTC TCTGGGTATC
    TTCAGCTATG CTGGCGTGTC ACTCACTCTT ATTGTCATTG AGGTGATCCC ATTTCTGGTG
    CTAGCTGTTG GAGTTGACAA CATATACATT ATTGTGCAGA GATATCAGAG AGATGAGCGT
    CTTCATGATG AGACTCTGGA CCAGCAGATT GGCAGAATAC TGGGAGATGT GGCGCCGAGT
    ATATTTCTGT CAGCTTTTGC CGAAACGGTT GCCTTTTTTT TGGGAGCATT GTCCTCTATG
    CCAGCCGTGC GGACATTTTC ACTCTTTGCT GGGATGGCCG TGTTTCTAGA CTTCCTCCTT
    CAGATCACCT GCTTCATCAG CCTTCTTAGT CTGGACATCA GGCGACAAGA GAAAAACCGC
    TTAGATATCC TTTGCTGTGT GCCTGGATGC AAGAGAAACC GTGGCACTGA CAAACCGAAG
    AGCTGGCTGT TTTTGTTCTT CAAAAAGCTT TATGCCCCTG TACTGATGAA GGATTGGATA
    AGACCTATTG TGGTATCCGT TTTTGTTGGA ATCCTGTCCT TTAGCATTGC GGTTGTCAAC
    AAAGTAGAAA TAGGTCTTGA TCAGTCTCTC TCCATGCCTG ATGATTCCTA CATGCTAGAT
    TATTTTGGCT CACTCAGCAC ATACCTCCAT ACTGGCCCCC CAGTGTATTT TGTAGTTGAG
    GAAGGACATA ATTATACCAC AAAGGAAGGA CAGGACAAGG TGTGTGGTGG AGCTGGATGC
    AATAACAATT CATTGGTGCA AGAGATCTAC ACCGCTGCTG GGATAAGCAA CTATACAAGG
    ATCGGATACG CACCTTCCTC TTGGATTGAC GATTACTTTG ATTGGGTGAA GCCCCAGTCT
    ACTTGCTGTC GAATATATAA TAACACAGAT CAATTCTGTA ATGCTTCAGT GGTTAACGCA
    TCCTGCTTAT CATGCCGGTC CTACACTCCA GAAGGAAAGC GCAAACCAGT TGGAGAGGAT
    TTTATGCATT TCCTGCCCAT GTTTCTCTCT GATAATCCAA ACCCCAAATG TGGGAAAGGG
    GGCCACGCGG CATATGGCTC TGCAGTTGAT CTCCTTGACA GTAACACTAA TGTTGGTGCA
    ACTTATTTTA TGACCTATCA TACAATTCTG AAGAACTCCA CAGACTTCAT TGACGCCATG
    AGGAAAGCCC GTACCATCGC ACAGAATATC ACTGATAATA TGGACATCCC AGGGAAGAAA
    TACAAAGTAT TCCCTTACAG TATTTTCTAT GTATTTTATG AGCAGTATTT AACCATTGTG
    GATGACACCA TCTTCAACTT GTGCGTGTCT CTGGCAGCAG TATTCTTTGT GACAGTGATC
    CTTCTGGGCT TTGAGCTCTG GTCAGCTGTC CTTATGTGCA TCACGATTTC TATGATCCTT
    GTTAACATGT TTGGAGTCAT GTGGCTCTGG GGCATCAGCT TAAATGCAGT GTCCCTTGTC
    AATTTAGTCA TGAGCTGTGG CATTTCAGTA GAGTTCTGCA GCCACGTGAC CAGAGCCTTT
    TCAGTTAGCA CAAGGGGAAA CCGGGTACAA CGTGCTGAGG ATGCCCTGGC TCATATGGGT
    AGCTCGGTGT TCAGTGGTAT AACACTCACA AAGTTTGTTG GTATCGGTGT TCTTGGCTTG
    TCCAAATCTC AGATATTCAA GATCTTCTAC TTCAGAATGT ATCTGGCTAT GGTGCTTCTT
    GGGGCAGCAC ATGGCTTAAT ATTTCTGCCT GTTCTACTTA GTTACTTAGG CCCCCAAGTG
    AATAAAGCAA AGGTGCAAGC TGCGCTGCAG AGAGCCCGAG GCACAGAGCA AGAGCGTCTT
    TTTAACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.