nf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa42785 XM_018091614.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa42786 XM_018091615.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OXa05706 XM_002936819.4
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa35805 XM_012957569.2
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa42780 XM_018091609.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa42781 XM_018091610.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa42782 XM_018091611.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa42783 XM_018091612.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa42784 XM_018091613.1
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa52430 XM_002936819.5
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa52431 XM_018091610.2
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa52432 XM_012957569.3
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa52433 XM_018091613.2
Latest version!
Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa42785
    Clone ID Related Accession (Same CDS sequence) XM_018091614.1
    Accession Version XM_018091614.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGCCTCACAG ATGTGCAGCA GAGTGATGGA GGGCCAGGGC
    AGCAGTACAC AGGAAATGGG GCATCATCCC ACTCGACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947103.1
    CDS276..6617
    Translation

    Target ORF information:

    RefSeq Version XM_018091614.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091614.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGCCTCACAG ATGTGCAGCA GAGTGATGGA GGGCCAGGGC
    AGCAGTACAC AGGAAATGGG GCATCATCCC ACTCGACATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42786
    Clone ID Related Accession (Same CDS sequence) XM_018091615.1
    Accession Version XM_018091615.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGCCTGTTTT GCAGTATGCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947104.1
    CDS276..6560
    Translation

    Target ORF information:

    RefSeq Version XM_018091615.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091615.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGCCTGTTTT GCAGTATGCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05706
    Clone ID Related Accession (Same CDS sequence) XM_002936819.4
    Accession Version XM_002936819.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCATC
    GACAAGGAAA ACGTGGAACT GTCTCCCACC ACAGGCCATT CAAACAGCAG TAGAACACGC
    CACGGTTCAG CAAGCCAAGT GCAAAAGCAG AGAAGTGCTG GCAGCTTCAA ACGTCACAGC
    ATTAAAAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936865.2
    CDS274..8631
    Translation

    Target ORF information:

    RefSeq Version XM_002936819.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936819.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCATC
    GACAAGGAAA ACGTGGAACT GTCTCCCACC ACAGGCCATT CAAACAGCAG TAGAACACGC
    CACGGTTCAG CAAGCCAAGT GCAAAAGCAG AGAAGTGCTG GCAGCTTCAA ACGTCACAGC
    ATTAAAAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35805
    Clone ID Related Accession (Same CDS sequence) XM_012957569.2
    Accession Version XM_012957569.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTCGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGGCATCGAC
    AAGGAAAACG TGGAACTGTC TCCCACCACA GGCCATTCAA ACAGCAGTAG AACACGCCAC
    GGTTCAGCAA GCCAAGTGCA AAAGCAGAGA AGTGCTGGCA GCTTCAAACG TCACAGCATT
    AAAAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813023.1
    CDS274..8568
    Translation

    Target ORF information:

    RefSeq Version XM_012957569.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTCGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGGCATCGAC
    AAGGAAAACG TGGAACTGTC TCCCACCACA GGCCATTCAA ACAGCAGTAG AACACGCCAC
    GGTTCAGCAA GCCAAGTGCA AAAGCAGAGA AGTGCTGGCA GCTTCAAACG TCACAGCATT
    AAAAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42780
    Clone ID Related Accession (Same CDS sequence) XM_018091609.1
    Accession Version XM_018091609.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CATCGACAAG
    GAAAACGTGG AACTGTCTCC CACCACAGGC CATTCAAACA GCAGTAGAAC ACGCCACGGT
    TCAGCAAGCC AAGTGCAAAA GCAGAGAAGT GCTGGCAGCT TCAAACGTCA CAGCATTAAA
    AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947098.1
    CDS274..8685
    Translation

    Target ORF information:

    RefSeq Version XM_018091609.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091609.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CATCGACAAG
    GAAAACGTGG AACTGTCTCC CACCACAGGC CATTCAAACA GCAGTAGAAC ACGCCACGGT
    TCAGCAAGCC AAGTGCAAAA GCAGAGAAGT GCTGGCAGCT TCAAACGTCA CAGCATTAAA
    AAGATTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42781
    Clone ID Related Accession (Same CDS sequence) XM_018091610.1
    Accession Version XM_018091610.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CAGTGTGGTT
    CAGTGCTGTC TGTGTTTAGG AACAGCTCTA GCAACAGTCC CCAAGAGAGA ATATGTCAAC
    TGGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947099.1
    CDS275..8623
    Translation

    Target ORF information:

    RefSeq Version XM_018091610.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091610.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CAGTGTGGTT
    CAGTGCTGTC TGTGTTTAGG AACAGCTCTA GCAACAGTCC CCAAGAGAGA ATATGTCAAC
    TGGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42782
    Clone ID Related Accession (Same CDS sequence) XM_018091611.1
    Accession Version XM_018091611.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
    2101
    2161
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    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTCGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGCTTCTCTG
    CCTTGCTCTA AATCAGCCGT TTTCATGCAG CCACTCCCTC ATCAAGGCAT CGACAAGGAA
    AACGTGGAAC TGTCTCCCAC CACAGGCCAT TCAAACAGCA GTAGAACACG CCACGGTTCA
    GCAAGCCAAG TGCAAAAGCA GAGAAGTGCT GGCAGCTTCA AACGTCACAG CATTAAAAAG
    ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947100.1
    CDS274..8622
    Translation

    Target ORF information:

    RefSeq Version XM_018091611.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091611.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTCGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGCTTCTCTG
    CCTTGCTCTA AATCAGCCGT TTTCATGCAG CCACTCCCTC ATCAAGGCAT CGACAAGGAA
    AACGTGGAAC TGTCTCCCAC CACAGGCCAT TCAAACAGCA GTAGAACACG CCACGGTTCA
    GCAAGCCAAG TGCAAAAGCA GAGAAGTGCT GGCAGCTTCA AACGTCACAG CATTAAAAAG
    ATTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42783
    Clone ID Related Accession (Same CDS sequence) XM_018091612.1
    Accession Version XM_018091612.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGAGCACA ACAAAGAGTG TCTCATAAAC ATTTCAAAGT ACAAATTTTC TCTGGTCATA 
    AGTGGACTTA CGAACATCTT AAAAAATGTA AACCACATGA GGATATCTGG AGAGACTTCA
    GAAAAAAATT TATATTTATC TCAGTTGATT ATTTTGGATA CACTGGATAA ATGTCTTGCA
    GGGCAACCAA AGGATTCTAT GCGGTTAGAT GAAACAATGC TGGTTAAACA GTTACTGCCA
    GAGATCTGCC ATTTTATTCA CACATACAGA GAAGGCAATC AGTATGCAGC CGAACTCCGA
    ATGTCTGCTT CGGGGGTACT GTTCTCACTT AGCTGTAATA ACTTTAATGC CGTCTTCAGC
    CGTATTTCTA CAAGGCTTCA GGAACTGACA GTTTGCTCAG AGGACAATGC TGATGTTCAC
    GATATAGACT TGATACAATA TATCAATGTG GACTGTTCAA AACTTAAACG ACTTTTACAG
    GAAACAGTCT TTAAATTTAA GTCTCTGAAG AAAGTTGCTC AGTTGGCAGT TATTAACAGT
    CTCGAAAAGG CCTTTTGGAA TTGGGTTGAA AATTATCCAG ATGAATTTAC AAAGTTGTAC
    CAACGACCAC AGACTGATAT GGCAGACTGT GCAGAAAAGC TTTTTGACTT GGTGGACAGC
    TTTGCCGAGA GCAACAAGCG CAAAGCAGCA GTCTGGCCAC TGCAGATAAT TTTGTTGGTT
    CTGTGTCCTG AAATTATTCA GGATATTTCC AAGGATGTTG TAGAAGAAAG TAAAATGAAC
    AAGAAACTAT TCCTTGAAAA CCTCAGAAAA GCTTTAGCTG GACATGGAGG AACAAAGTCA
    CTCACAGAAA CTGCAGCCAT TGCTAGTGTC AAACTGTGCA AAGCCTCGAC ATACATCAAC
    TGGGAAGATA ATTCTGTCAT ATTTCACCTT GTGCAGTCTT TAGTCATTGA CTTAAAGAAT
    TTGCTGTTTA ATCCAAGCAA ACCTTTCTCC AGAGGAGCAG TGAGCCAGAA TGCTGATTTG
    GATCTTATGA TTGACTGCCT GGTTTCTTGT TTTCGAATCA ACCCTCACAA CAACCAGCAC
    TTTAAAACCT GTTTGGCGCA GTCATCACCA ACAACATTTC ATTATGTGCT TGTAAATTCC
    CTGCATAGAA TAATTACAAA TTCTGCATTG GATTGGTGGC CTAAGATTGA TGCTGTGTAT
    TGCTACTCAG GAGAACTGCG CAGCATGTTT TCTGAAACTC TCAAACTTGT TATGCAGCAG
    ACAGCAAGCC GCCTGACGCC GAGTCTAACT TTTAAGGAAA AAATGCCATA CCTGAAATTT
    AAGGACAGGT TAGCTGACCC AGAAACCAAG AGTTACAAGT ACTTGCTGCT GTCTGTGGTA
    AAGCTCATTC ATGGAGATCC AAAGCTGCTC TTATATAATC CTGGAAAAGC AGTTTCTGAT
    ACACAAAGCA GTACTGCAGA ATTAATTAAT GGACTGGTAC AGCTGGTGCC TCAGTCAAAT
    ATGCCAGAAA TTGCACAGGA AGCTATGGAG GCTCTGTTGG TTCTTCATCA GTCAGACAGC
    ATTGAGCTTT GGAATCCAGA GGCTCCAATA GAAACATTCT GGGAAATAAG CTCCCAAATG
    CTTTATTCCA TCTGTACCAA GCTAACTGGT CACCAGTTGC TTAACAGCAC AGAAATTTTA
    AAATGGCTGC GAGAAATCCT GATATGTAGG AATAAATTTC TTCTTAAATA TAAGGAAAGT
    GCTGCTGCGG GAAGTGGAAT CCCAATATGC CGTCAAGCCC AGACAAAATT AGAAGTTGCA
    CTTTACATGT TCCTCTGGAG TCCTGACACT GAGGCAGTTC TTGTTGCAAT GTCCTGTTTT
    CGAAGTTTGT GTGAAGAGGC AGACATCCGG TGTGGGGTTG ATGAAGTATC TGTTCATAAT
    TTTTTGCCAA ATTACAACAC ATTTATGGAG TTTGCATCAG TAAGCAACAT GGTGTCGACT
    GGGAGAGCAG CTCTGCAGAA GCGAGTTATG GCCTTATTAA GAAGAATAGA ACACCCTACT
    GCAGGAAACA CGGAGGCTTG GGAAGATACA CATGCAAAAT GGGATCATGC CACAAGACTG
    ATTCTAAACT ACCCCAAAAC TAAATTGGAG GATGGGCAGG CCCCAGAAAG CCTTCATAAA
    ACAATTGTGA AAAGGAGAAT GTCTCATGTA AGTGGAGGGG GATCTATAGA CCTGTCTGAC
    ACAGATTGTC TGCAGGAATG GATCAACATG ACTGGTTTCC TTTCTGCTCT TGGAGGAGTC
    TGCCTTCAAC ATCGAGGCAA TGCTGGACTA GTTACTTACA GTCCACCCAT GGGTCCAGTT
    AATGAACGCA AAGGTTCTGT AATATCAATG GTTTCCACTG AAGGAAATGC AGAAACGCCT
    GTCAGCAAAT TTCTTGATCG CTTACTGTCT CTGATGGTGT GCAACCATGA AAAAGTGGGG
    ATACAAATTC GAAATAACAT TAAGGATTTA GTTGGTTTGG AGTTAAGCCC GGCTCTCTAT
    CCAATGTTAT TTAATAAAAT GAAGAACAAC ATAAGCAAAT TTTTTGACTC TCAAGGACAG
    GTTCTCCTAA CGGATACAAA CACCCAGTTT GTCGAGCAGA CAATTACCAT ACTGAAGAAC
    TTGCTTGATA ATCACACAGA AGGCAGTTCA GAGCATTTGG GCCAAGCAAG TCTTGAAACA
    ATGATGCTAA ATTTAGTAAG GTACGTCCGG GTGCTAGGCA ACTTGGTCCA TGCCATCCAG
    ATTAAAACAA AGCTCTGTCA GTTGGTGGAA GTTATGATGG AAAGAAGAGA TGACCTCTCA
    TTCTGTCAAG AGATGAAATT TAGGAACAAA ATGGTGGAGT ACTTAACTGA CTGGGTAATG
    GGAACATCCA ATCAGGCAGC GGATGAAGAT GTAAAATGCC TGACAAGAGA CTTAGATCAG
    GCCAGCATGG AAGCAGTTGT ATCCCTACTC GCAGGTCTGC CTCTGCAGCC AGAGGAAGGG
    GATGGGGTGG AGTTAATGGA GGCAAAATCT CAGCTTTTCC TGAAGTACTT CACGTTGTTC
    ATGAACTTGT TAAATGACTG CAGCGAGGCT GAAGATGATG GAACCCAGAC CGGTGGTCGA
    AAAAGGGGGA TGTCTAGGAG ACTGGCTTCC TTAAGGCACT GTACTGTCCT GGCCATGTCT
    AATTTGCTTA ATGCTAATGT GGATAGTGGC CTTATGCATT CAATAGGGCT TGGCTATCAC
    AAGGATCTGC AGACAAGAGC GACATTTATG GAGGTTCTAA CAAAAATACT ACAGCAAGGA
    ACAGAGTTTG ATACACTTGC TGAAACTGTT CTGGCTGATC GTTTTGAAAG ACTAGTAGAA
    TTGGTGACCA TGATGGGAGA TCAAGGCGAA TTACCTATTG CAATGGCTTT AGCCAATGTT
    GTTCCATGCT CACAGTGGGA TGAGCTTGCT CGAGTTCTTG TGACACTTTT CGATTCTCGT
    CACCTACTCT ACCAGCTTTT ATGGAATATG TTTTCTAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TGTTCAGAGG AAACAGCTTG GCTAGTAAAA TAATGACATT TTGCTTTAAG
    GTCTATGGTG CCACATATCT ACAAAAGCTT CTGGAACCTT TACTAAGGAC TTTCATTACA
    TCACCTGAGT GGCAATATGT CAGTTTTGAG GTTGACTCAA CAAGGCTGGA GGGAAATGAA
    CTCTTGGAAG AAAATCAACG TCGCCTCCTA CAGATGACTG AGAAATTCTT TCATGCCATT
    ATTAACTCCA CAAATGAGTT CCCAACACAG CTTCGTAGTG TGTGCCATTG TCTGTACCAG
    GCAACTTGCC ACTCCCTACT GAGTAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTGA GTCAGAGATT TCCACAAAAT AGCATTGGTG CAGTGGGAAG TGCAATGTTC
    CTCAGGTTCA TAAATCCAGC CATTGTGTCA CCATACGAAG CTGGAATCTT AGATAAGAAG
    CCACCACCAC GGCTTGAGCG TGGTTTGAAG TTAATGTCAA AGATTTTACA AAGCATTGCT
    AATCATGTTC TGTTCACTAA AGAGGAACAC ATGCGTCCTT TTAATGAATT TGTCAAAAGT
    AACTTTGATG CTGCCAGGAG GTTCTTCTTT GACATAGCCA ACAATTGCCC TCCTAGCGAC
    ACGGTAAATC ACAGCCTCTC GTTTATAACT GATGGGAATG TACTCGCCTT ACATCGGTTG
    CTCTGGAACA ACCAGGAAAA GATTGGACAG TATCTATCCA GTAACAGGGA CCACAAAGCT
    GTAGGAAGAA GACCTTTTGA CAAAATGGCA ACTCTTCTTG CGTACCTTGG ACCTCCCGAA
    CACAAACCTG TAGCTGATAC ACATTGGTCA AGTCTTAACT TAACAAGCTC CAAGTTTGAA
    GAGTTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAAT TTAAAGCATT AAAAACCCTA
    AATATATTTT ACCAAGCAGG GACATCAAGA GCTGGTAATC CAGTTTTCTA CTATATTGCT
    AGAAGGTTCA AGACTGGTCA GATCAATGGT GACTTACTGA TTTACCACGT CTTGTTGACT
    CTAAAGCCAT ACTATGCCAA GCCATATGAA ATAGTGGTAG ATCTCACCCA TGCTGGGCCC
    AGCAATCGCT TCAAGACAGA CTTCCTTTCC AAATGGTTTG TGGTCTTTCC TGGTTTCGCT
    TATGAAAATG TGACTGCAGT TTATATTTAC AACTGCAACT CTTGGGTCAG GGAATACACT
    AAATACCATG AAAGGCTGTT AACCGGCCTA AAAGGCAGCA AAAGGCTAAT TTTCATTGAT
    CCATCTGGAA AGTTAACAGA GTATATTGAA CATGAACAGC AAAAACTCCC AGCAGCCACA
    TTGGCATTGG AAGAAGACCT AAAGTTGTTC CATAATGCAC TCAAATTAGC CCACAAAGAT
    ACCAAGGTCT CCATAAAAGT TGGTTCTACA GCTGTTCAGG TAACGTCAGC AGAGAGAACT
    CGAGTCCTCG GGCAGTCAGT CTTTTTGAAT GACATTTATT ACGCATCTGA GATTGAGGAG
    ATTTGTTTGG TGGATGAAAA CCAGTTTACT TTAACTATCG CCAACCAAGG AACCCCTCTC
    ACTTTCATGC ACCAGGAGTG TGAAGCCATC GTCCAATCTA TCATTCACAT ACGGACTCGA
    TGGGAACTGT CACAACCAGA TTCCATTCCT CAGCACACTA AAATCCGTCC AAAGGATGTA
    CCCGGAACAC TACTAAATAT TGCTTTATTA AATCTGGGAA GTTCAGACCC AAGTCTACGG
    TCTGCTGCAT ATAACCTTCT GTGTGCCTTA ACATGTACCT TTAATTTAAA GATTGAAGGG
    CAGTTACTAG AGACTTCGGG TCTGTGCATT CCTGCCAACA ATACTCTCTT CATAGTTTCC
    ATTAGTAAGA CACTGGCAGC TAATGAGCCA CATCTCACTC TTGAGTTTTT AGAAGAGTGC
    ATCTCTGGCT TCAGCAAATC CAGTATCGAA CTGAAGCATC TGTGTCTTGA ATACATGACG
    CCTTGGCTGC TAAACCTTGT TCGGTTCTGT AAACTAACAG ATGATGCTAA ACGTCAGCGT
    GTTAGTGCTA TTTTGGACAA ACTTATAACC ATGACTATAA ATGAGAAACA AATGTATCCA
    TCCATACAAG CGAAAATATG GGGCAGTCTT GGCCAGATCA CTGATCTCCT TGATGTTGTC
    CTGGACAGCT TTATCAAAAC CAGTGCAACG GGTGGCTTGG GTTCCATTAA GGCTGAGGTT
    ATGGCCGATA CAGCTGTTGC GCTTGCTTCG GGCAATGTGA AACTGGTTTC AAGCAAGGTT
    ATTGGGAGAA TGTGTAAAAT AATAGATAAG ACCTGCCTCT CCCCAACACC CACCCTTGAG
    CAGCATCTTA TGTGGGATGA CATTGCCATT CTTGCCCGGT ACATGCTGAT GCTGTCTTTT
    AACAACTCTT TGGATGTGGC CGCCCATCTT CCCTACCTCT TCCATGTTGT CACTCTGTTA
    GTGGCAACTG GCCCGCTGTC TCTTCGAGCT TCCACCCATG GGCTTGTCAT CAATATCATT
    CACTCCTTGT GCACTTGCTC TCAGCTGAAT TTCAGTGAGG AAACTAAACA GGTATTAAGG
    CTAAGCCTGA CTGAATTCTC ACTGCCCAAG TTTTACCTGT TGTTTGGAAT AAGCAAGGTT
    AAATCTGCAG CTGTGATAGC CTTCAGATCG AGTTATCGGG ATCGATCATT CTCACCTGGC
    TCTTATGAGA GGGAAACATT TGCTTTGACC TCATTGGAAA CTGTTACAGA AGCCTTATTG
    GAAATTATGG AGGCTTGCAT GAGAGATATC CCAACTTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AGAAGTTTGC CTTTCAGTAT AACCCATCAC TACAGCCACG AGCTCTTGTA
    GTTTTTGGGT GCATTAGCAA ACGGGTTTCT CATGGCCAAA TCAAACAGAT CATACGAATA
    CTTAGCAAGG GGCTTGAAAG CTGCTTGAAA GGTCCTGACA ATTACAACAG CCAAGTTTTA
    ATTGAAGCCA CCGTGATAGC ACTAACGAAA CTGCAGCCTC TCCTGAATAA GGACTCTCCA
    ATGCACAAAG CTCTCTTCTG GGTGGCTATG GCAGTATTGC AGCTGGATGA AGTTAATCTC
    TACTCAGCAG GAACAGCTCT CCTCGAACAA AATTTGCACA CTTTAGATAG CCTTAGAGTG
    TTCAATGACA AAAGTCCAGA AGAAGTTTTT ATGGAAATAA GAAATCCATT GGAGGAATGG
    CATTGTAAAC AAATGGACCA TTTTGTTGGG CTCAACTTTA ACTCTAATTT TAATTTTGCA
    CTAGTCGGAC ATTTACTCAA AGGTTATCGA CACCCATCTC CTACAACTGT GGCAAGGACA
    ATACGAATTC TTCACACCCT CTTAGCACTT GTCAACAAAC ACAGAAATTG TGATAAGTTT
    GAAGTGAACA CTGAGAGTGT TGCATACCTG GCAGCTTTGC TCACTGTTTC TGAAGAGGTG
    AGGAGCCGAT GCAGCCTGAA ACACAGAAAA TCTCTTTTAC TGACAGATGT TTCAATGGAG
    AATGTTCCTA TGGATACATA TCCAGTGCAT CATAATGACC CCACATGCAG AACATTGAAG
    GAAAACCAGC CCTGGTCATC ACCACAAGCA TCTGGTATGC ACCTAGCTGC TAGCTACCCA
    ACAGTGGGGC AGGTTAGCCC ACGAACCAGG AAGTCCATGA GCCTCGACAT GGGGCAACCA
    TCACAAGCAA ACACTAAAAA GCTTCTTGGC ACAAGGAAAA GCTTTGACCA CTTGATATCT
    GACACTAAGG CTCCTAAAAG ACAGGAGATG GAGTCTGGCA TCACTACACC ACCTAAGATG
    AGGAGAGTTG CAGAGCATGA TTATGATATT GACACACCAA GAATATCATC CCCGCAACAG
    CACCCTCACC TCCGGAAAGT ATCTGTGTCT GAGTCCACTG TACTTTTGGA TGAGGAGGTC
    CTGACGGATC CAAAAACTCA AGCTTTGCTC CTCACTGTTC TTGCTACACT TGTAAAATAC
    ACTACAGATG AGTTTGATCA ACGCATCCTG TATGAGTATT TAGCAGAAGC CAGTGTGGTT
    TTTCCTAAAG TATTCCCTGT GGTACATAAT TTGTTGGATT CTAAGATAAG CACACTTCTA
    TCTCTGTGCC AAGACCCAAA TTTATTAAAT CCAATTCATG GGATTGTTCA GAGTGTGGTG
    TACCATGAAG AGTCCCCTCC ACAGTATCAG CCATCATATT TACAAAGTTT TGGTTTTAAT
    GGTTTGTGGA GGTTTGCTGG ACCTTTCTCC AAGCAAACGC AAATCCCTGA CTACGCAGAG
    CTGATAGTAA AATTTCTTGA TGCCTTGATT GATAAATATT TGCCAGTAAT TGATGAAGAA
    GCCAGTGAAG AATCTCTCCT GACTCCTACG TCTCCTTACC CTCCTGCTGT GCAGAGTCAA
    TTGAGTATTA CTGCTAACCT TAATCTTTCC AACTCAATGA CCTCCCTTGC AACTTCCCAG
    CATTCCCCAG CTTCTCTGCC TTGCTCTAAA TCAGCCGTTT TCATGCAGCC ACTCCCTCAT
    CAAGGCATCG ACAAGGAAAA CGTGGAACTG TCTCCCACCA CAGGCCATTC AAACAGCAGT
    AGAACACGCC ACGGTTCAGC AAGCCAAGTG CAAAAGCAGA GAAGTGCTGG CAGCTTCAAA
    CGTCACAGCA TTAAAAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947101.1
    CDS339..8645
    Translation

    Target ORF information:

    RefSeq Version XM_018091612.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091612.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGAGCACA ACAAAGAGTG TCTCATAAAC ATTTCAAAGT ACAAATTTTC TCTGGTCATA 
    AGTGGACTTA CGAACATCTT AAAAAATGTA AACCACATGA GGATATCTGG AGAGACTTCA
    GAAAAAAATT TATATTTATC TCAGTTGATT ATTTTGGATA CACTGGATAA ATGTCTTGCA
    GGGCAACCAA AGGATTCTAT GCGGTTAGAT GAAACAATGC TGGTTAAACA GTTACTGCCA
    GAGATCTGCC ATTTTATTCA CACATACAGA GAAGGCAATC AGTATGCAGC CGAACTCCGA
    ATGTCTGCTT CGGGGGTACT GTTCTCACTT AGCTGTAATA ACTTTAATGC CGTCTTCAGC
    CGTATTTCTA CAAGGCTTCA GGAACTGACA GTTTGCTCAG AGGACAATGC TGATGTTCAC
    GATATAGACT TGATACAATA TATCAATGTG GACTGTTCAA AACTTAAACG ACTTTTACAG
    GAAACAGTCT TTAAATTTAA GTCTCTGAAG AAAGTTGCTC AGTTGGCAGT TATTAACAGT
    CTCGAAAAGG CCTTTTGGAA TTGGGTTGAA AATTATCCAG ATGAATTTAC AAAGTTGTAC
    CAACGACCAC AGACTGATAT GGCAGACTGT GCAGAAAAGC TTTTTGACTT GGTGGACAGC
    TTTGCCGAGA GCAACAAGCG CAAAGCAGCA GTCTGGCCAC TGCAGATAAT TTTGTTGGTT
    CTGTGTCCTG AAATTATTCA GGATATTTCC AAGGATGTTG TAGAAGAAAG TAAAATGAAC
    AAGAAACTAT TCCTTGAAAA CCTCAGAAAA GCTTTAGCTG GACATGGAGG AACAAAGTCA
    CTCACAGAAA CTGCAGCCAT TGCTAGTGTC AAACTGTGCA AAGCCTCGAC ATACATCAAC
    TGGGAAGATA ATTCTGTCAT ATTTCACCTT GTGCAGTCTT TAGTCATTGA CTTAAAGAAT
    TTGCTGTTTA ATCCAAGCAA ACCTTTCTCC AGAGGAGCAG TGAGCCAGAA TGCTGATTTG
    GATCTTATGA TTGACTGCCT GGTTTCTTGT TTTCGAATCA ACCCTCACAA CAACCAGCAC
    TTTAAAACCT GTTTGGCGCA GTCATCACCA ACAACATTTC ATTATGTGCT TGTAAATTCC
    CTGCATAGAA TAATTACAAA TTCTGCATTG GATTGGTGGC CTAAGATTGA TGCTGTGTAT
    TGCTACTCAG GAGAACTGCG CAGCATGTTT TCTGAAACTC TCAAACTTGT TATGCAGCAG
    ACAGCAAGCC GCCTGACGCC GAGTCTAACT TTTAAGGAAA AAATGCCATA CCTGAAATTT
    AAGGACAGGT TAGCTGACCC AGAAACCAAG AGTTACAAGT ACTTGCTGCT GTCTGTGGTA
    AAGCTCATTC ATGGAGATCC AAAGCTGCTC TTATATAATC CTGGAAAAGC AGTTTCTGAT
    ACACAAAGCA GTACTGCAGA ATTAATTAAT GGACTGGTAC AGCTGGTGCC TCAGTCAAAT
    ATGCCAGAAA TTGCACAGGA AGCTATGGAG GCTCTGTTGG TTCTTCATCA GTCAGACAGC
    ATTGAGCTTT GGAATCCAGA GGCTCCAATA GAAACATTCT GGGAAATAAG CTCCCAAATG
    CTTTATTCCA TCTGTACCAA GCTAACTGGT CACCAGTTGC TTAACAGCAC AGAAATTTTA
    AAATGGCTGC GAGAAATCCT GATATGTAGG AATAAATTTC TTCTTAAATA TAAGGAAAGT
    GCTGCTGCGG GAAGTGGAAT CCCAATATGC CGTCAAGCCC AGACAAAATT AGAAGTTGCA
    CTTTACATGT TCCTCTGGAG TCCTGACACT GAGGCAGTTC TTGTTGCAAT GTCCTGTTTT
    CGAAGTTTGT GTGAAGAGGC AGACATCCGG TGTGGGGTTG ATGAAGTATC TGTTCATAAT
    TTTTTGCCAA ATTACAACAC ATTTATGGAG TTTGCATCAG TAAGCAACAT GGTGTCGACT
    GGGAGAGCAG CTCTGCAGAA GCGAGTTATG GCCTTATTAA GAAGAATAGA ACACCCTACT
    GCAGGAAACA CGGAGGCTTG GGAAGATACA CATGCAAAAT GGGATCATGC CACAAGACTG
    ATTCTAAACT ACCCCAAAAC TAAATTGGAG GATGGGCAGG CCCCAGAAAG CCTTCATAAA
    ACAATTGTGA AAAGGAGAAT GTCTCATGTA AGTGGAGGGG GATCTATAGA CCTGTCTGAC
    ACAGATTGTC TGCAGGAATG GATCAACATG ACTGGTTTCC TTTCTGCTCT TGGAGGAGTC
    TGCCTTCAAC ATCGAGGCAA TGCTGGACTA GTTACTTACA GTCCACCCAT GGGTCCAGTT
    AATGAACGCA AAGGTTCTGT AATATCAATG GTTTCCACTG AAGGAAATGC AGAAACGCCT
    GTCAGCAAAT TTCTTGATCG CTTACTGTCT CTGATGGTGT GCAACCATGA AAAAGTGGGG
    ATACAAATTC GAAATAACAT TAAGGATTTA GTTGGTTTGG AGTTAAGCCC GGCTCTCTAT
    CCAATGTTAT TTAATAAAAT GAAGAACAAC ATAAGCAAAT TTTTTGACTC TCAAGGACAG
    GTTCTCCTAA CGGATACAAA CACCCAGTTT GTCGAGCAGA CAATTACCAT ACTGAAGAAC
    TTGCTTGATA ATCACACAGA AGGCAGTTCA GAGCATTTGG GCCAAGCAAG TCTTGAAACA
    ATGATGCTAA ATTTAGTAAG GTACGTCCGG GTGCTAGGCA ACTTGGTCCA TGCCATCCAG
    ATTAAAACAA AGCTCTGTCA GTTGGTGGAA GTTATGATGG AAAGAAGAGA TGACCTCTCA
    TTCTGTCAAG AGATGAAATT TAGGAACAAA ATGGTGGAGT ACTTAACTGA CTGGGTAATG
    GGAACATCCA ATCAGGCAGC GGATGAAGAT GTAAAATGCC TGACAAGAGA CTTAGATCAG
    GCCAGCATGG AAGCAGTTGT ATCCCTACTC GCAGGTCTGC CTCTGCAGCC AGAGGAAGGG
    GATGGGGTGG AGTTAATGGA GGCAAAATCT CAGCTTTTCC TGAAGTACTT CACGTTGTTC
    ATGAACTTGT TAAATGACTG CAGCGAGGCT GAAGATGATG GAACCCAGAC CGGTGGTCGA
    AAAAGGGGGA TGTCTAGGAG ACTGGCTTCC TTAAGGCACT GTACTGTCCT GGCCATGTCT
    AATTTGCTTA ATGCTAATGT GGATAGTGGC CTTATGCATT CAATAGGGCT TGGCTATCAC
    AAGGATCTGC AGACAAGAGC GACATTTATG GAGGTTCTAA CAAAAATACT ACAGCAAGGA
    ACAGAGTTTG ATACACTTGC TGAAACTGTT CTGGCTGATC GTTTTGAAAG ACTAGTAGAA
    TTGGTGACCA TGATGGGAGA TCAAGGCGAA TTACCTATTG CAATGGCTTT AGCCAATGTT
    GTTCCATGCT CACAGTGGGA TGAGCTTGCT CGAGTTCTTG TGACACTTTT CGATTCTCGT
    CACCTACTCT ACCAGCTTTT ATGGAATATG TTTTCTAAGG AAGTTGAACT GGCAGATTCC
    ATGCAGACAC TGTTCAGAGG AAACAGCTTG GCTAGTAAAA TAATGACATT TTGCTTTAAG
    GTCTATGGTG CCACATATCT ACAAAAGCTT CTGGAACCTT TACTAAGGAC TTTCATTACA
    TCACCTGAGT GGCAATATGT CAGTTTTGAG GTTGACTCAA CAAGGCTGGA GGGAAATGAA
    CTCTTGGAAG AAAATCAACG TCGCCTCCTA CAGATGACTG AGAAATTCTT TCATGCCATT
    ATTAACTCCA CAAATGAGTT CCCAACACAG CTTCGTAGTG TGTGCCATTG TCTGTACCAG
    GCAACTTGCC ACTCCCTACT GAGTAAAGCT ACCGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTTGTGA GTCAGAGATT TCCACAAAAT AGCATTGGTG CAGTGGGAAG TGCAATGTTC
    CTCAGGTTCA TAAATCCAGC CATTGTGTCA CCATACGAAG CTGGAATCTT AGATAAGAAG
    CCACCACCAC GGCTTGAGCG TGGTTTGAAG TTAATGTCAA AGATTTTACA AAGCATTGCT
    AATCATGTTC TGTTCACTAA AGAGGAACAC ATGCGTCCTT TTAATGAATT TGTCAAAAGT
    AACTTTGATG CTGCCAGGAG GTTCTTCTTT GACATAGCCA ACAATTGCCC TCCTAGCGAC
    ACGGTAAATC ACAGCCTCTC GTTTATAACT GATGGGAATG TACTCGCCTT ACATCGGTTG
    CTCTGGAACA ACCAGGAAAA GATTGGACAG TATCTATCCA GTAACAGGGA CCACAAAGCT
    GTAGGAAGAA GACCTTTTGA CAAAATGGCA ACTCTTCTTG CGTACCTTGG ACCTCCCGAA
    CACAAACCTG TAGCTGATAC ACATTGGTCA AGTCTTAACT TAACAAGCTC CAAGTTTGAA
    GAGTTTATGA CAAGGCATCA GGTACATGAA AAAGAAGAAT TTAAAGCATT AAAAACCCTA
    AATATATTTT ACCAAGCAGG GACATCAAGA GCTGGTAATC CAGTTTTCTA CTATATTGCT
    AGAAGGTTCA AGACTGGTCA GATCAATGGT GACTTACTGA TTTACCACGT CTTGTTGACT
    CTAAAGCCAT ACTATGCCAA GCCATATGAA ATAGTGGTAG ATCTCACCCA TGCTGGGCCC
    AGCAATCGCT TCAAGACAGA CTTCCTTTCC AAATGGTTTG TGGTCTTTCC TGGTTTCGCT
    TATGAAAATG TGACTGCAGT TTATATTTAC AACTGCAACT CTTGGGTCAG GGAATACACT
    AAATACCATG AAAGGCTGTT AACCGGCCTA AAAGGCAGCA AAAGGCTAAT TTTCATTGAT
    CCATCTGGAA AGTTAACAGA GTATATTGAA CATGAACAGC AAAAACTCCC AGCAGCCACA
    TTGGCATTGG AAGAAGACCT AAAGTTGTTC CATAATGCAC TCAAATTAGC CCACAAAGAT
    ACCAAGGTCT CCATAAAAGT TGGTTCTACA GCTGTTCAGG TAACGTCAGC AGAGAGAACT
    CGAGTCCTCG GGCAGTCAGT CTTTTTGAAT GACATTTATT ACGCATCTGA GATTGAGGAG
    ATTTGTTTGG TGGATGAAAA CCAGTTTACT TTAACTATCG CCAACCAAGG AACCCCTCTC
    ACTTTCATGC ACCAGGAGTG TGAAGCCATC GTCCAATCTA TCATTCACAT ACGGACTCGA
    TGGGAACTGT CACAACCAGA TTCCATTCCT CAGCACACTA AAATCCGTCC AAAGGATGTA
    CCCGGAACAC TACTAAATAT TGCTTTATTA AATCTGGGAA GTTCAGACCC AAGTCTACGG
    TCTGCTGCAT ATAACCTTCT GTGTGCCTTA ACATGTACCT TTAATTTAAA GATTGAAGGG
    CAGTTACTAG AGACTTCGGG TCTGTGCATT CCTGCCAACA ATACTCTCTT CATAGTTTCC
    ATTAGTAAGA CACTGGCAGC TAATGAGCCA CATCTCACTC TTGAGTTTTT AGAAGAGTGC
    ATCTCTGGCT TCAGCAAATC CAGTATCGAA CTGAAGCATC TGTGTCTTGA ATACATGACG
    CCTTGGCTGC TAAACCTTGT TCGGTTCTGT AAACTAACAG ATGATGCTAA ACGTCAGCGT
    GTTAGTGCTA TTTTGGACAA ACTTATAACC ATGACTATAA ATGAGAAACA AATGTATCCA
    TCCATACAAG CGAAAATATG GGGCAGTCTT GGCCAGATCA CTGATCTCCT TGATGTTGTC
    CTGGACAGCT TTATCAAAAC CAGTGCAACG GGTGGCTTGG GTTCCATTAA GGCTGAGGTT
    ATGGCCGATA CAGCTGTTGC GCTTGCTTCG GGCAATGTGA AACTGGTTTC AAGCAAGGTT
    ATTGGGAGAA TGTGTAAAAT AATAGATAAG ACCTGCCTCT CCCCAACACC CACCCTTGAG
    CAGCATCTTA TGTGGGATGA CATTGCCATT CTTGCCCGGT ACATGCTGAT GCTGTCTTTT
    AACAACTCTT TGGATGTGGC CGCCCATCTT CCCTACCTCT TCCATGTTGT CACTCTGTTA
    GTGGCAACTG GCCCGCTGTC TCTTCGAGCT TCCACCCATG GGCTTGTCAT CAATATCATT
    CACTCCTTGT GCACTTGCTC TCAGCTGAAT TTCAGTGAGG AAACTAAACA GGTATTAAGG
    CTAAGCCTGA CTGAATTCTC ACTGCCCAAG TTTTACCTGT TGTTTGGAAT AAGCAAGGTT
    AAATCTGCAG CTGTGATAGC CTTCAGATCG AGTTATCGGG ATCGATCATT CTCACCTGGC
    TCTTATGAGA GGGAAACATT TGCTTTGACC TCATTGGAAA CTGTTACAGA AGCCTTATTG
    GAAATTATGG AGGCTTGCAT GAGAGATATC CCAACTTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AGAAGTTTGC CTTTCAGTAT AACCCATCAC TACAGCCACG AGCTCTTGTA
    GTTTTTGGGT GCATTAGCAA ACGGGTTTCT CATGGCCAAA TCAAACAGAT CATACGAATA
    CTTAGCAAGG GGCTTGAAAG CTGCTTGAAA GGTCCTGACA ATTACAACAG CCAAGTTTTA
    ATTGAAGCCA CCGTGATAGC ACTAACGAAA CTGCAGCCTC TCCTGAATAA GGACTCTCCA
    ATGCACAAAG CTCTCTTCTG GGTGGCTATG GCAGTATTGC AGCTGGATGA AGTTAATCTC
    TACTCAGCAG GAACAGCTCT CCTCGAACAA AATTTGCACA CTTTAGATAG CCTTAGAGTG
    TTCAATGACA AAAGTCCAGA AGAAGTTTTT ATGGAAATAA GAAATCCATT GGAGGAATGG
    CATTGTAAAC AAATGGACCA TTTTGTTGGG CTCAACTTTA ACTCTAATTT TAATTTTGCA
    CTAGTCGGAC ATTTACTCAA AGGTTATCGA CACCCATCTC CTACAACTGT GGCAAGGACA
    ATACGAATTC TTCACACCCT CTTAGCACTT GTCAACAAAC ACAGAAATTG TGATAAGTTT
    GAAGTGAACA CTGAGAGTGT TGCATACCTG GCAGCTTTGC TCACTGTTTC TGAAGAGGTG
    AGGAGCCGAT GCAGCCTGAA ACACAGAAAA TCTCTTTTAC TGACAGATGT TTCAATGGAG
    AATGTTCCTA TGGATACATA TCCAGTGCAT CATAATGACC CCACATGCAG AACATTGAAG
    GAAAACCAGC CCTGGTCATC ACCACAAGCA TCTGGTATGC ACCTAGCTGC TAGCTACCCA
    ACAGTGGGGC AGGTTAGCCC ACGAACCAGG AAGTCCATGA GCCTCGACAT GGGGCAACCA
    TCACAAGCAA ACACTAAAAA GCTTCTTGGC ACAAGGAAAA GCTTTGACCA CTTGATATCT
    GACACTAAGG CTCCTAAAAG ACAGGAGATG GAGTCTGGCA TCACTACACC ACCTAAGATG
    AGGAGAGTTG CAGAGCATGA TTATGATATT GACACACCAA GAATATCATC CCCGCAACAG
    CACCCTCACC TCCGGAAAGT ATCTGTGTCT GAGTCCACTG TACTTTTGGA TGAGGAGGTC
    CTGACGGATC CAAAAACTCA AGCTTTGCTC CTCACTGTTC TTGCTACACT TGTAAAATAC
    ACTACAGATG AGTTTGATCA ACGCATCCTG TATGAGTATT TAGCAGAAGC CAGTGTGGTT
    TTTCCTAAAG TATTCCCTGT GGTACATAAT TTGTTGGATT CTAAGATAAG CACACTTCTA
    TCTCTGTGCC AAGACCCAAA TTTATTAAAT CCAATTCATG GGATTGTTCA GAGTGTGGTG
    TACCATGAAG AGTCCCCTCC ACAGTATCAG CCATCATATT TACAAAGTTT TGGTTTTAAT
    GGTTTGTGGA GGTTTGCTGG ACCTTTCTCC AAGCAAACGC AAATCCCTGA CTACGCAGAG
    CTGATAGTAA AATTTCTTGA TGCCTTGATT GATAAATATT TGCCAGTAAT TGATGAAGAA
    GCCAGTGAAG AATCTCTCCT GACTCCTACG TCTCCTTACC CTCCTGCTGT GCAGAGTCAA
    TTGAGTATTA CTGCTAACCT TAATCTTTCC AACTCAATGA CCTCCCTTGC AACTTCCCAG
    CATTCCCCAG CTTCTCTGCC TTGCTCTAAA TCAGCCGTTT TCATGCAGCC ACTCCCTCAT
    CAAGGCATCG ACAAGGAAAA CGTGGAACTG TCTCCCACCA CAGGCCATTC AAACAGCAGT
    AGAACACGCC ACGGTTCAGC AAGCCAAGTG CAAAAGCAGA GAAGTGCTGG CAGCTTCAAA
    CGTCACAGCA TTAAAAAGAT TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42784
    Clone ID Related Accession (Same CDS sequence) XM_018091613.1
    Accession Version XM_018091613.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCAGT
    GTGGTTCAGT GCTGTCTGTG TTTAGGAACA GCTCTAGCAA CAGTCCCCAA GAGAGAATAT
    GTCAACTGGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947102.1
    CDS274..8568
    Translation

    Target ORF information:

    RefSeq Version XM_018091613.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091613.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA GTTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTGGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTCG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCAGT
    GTGGTTCAGT GCTGTCTGTG TTTAGGAACA GCTCTAGCAA CAGTCCCCAA GAGAGAATAT
    GTCAACTGGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52430
    Clone ID Related Accession (Same CDS sequence) XM_002936819.5
    Accession Version XM_002936819.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936819.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCATC
    GACAAGGAGA ACGTGGAACT GTCTCCCACC ACAGGCCATT CAAACAGCAG TAGAACACGC
    CACGGTTCAG CAAGCCAAGT GCAAAAGCAG AGAAGTGCTG GCAGCTTCAA ACGTCACAGC
    ATTAAAAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936865.2
    CDS257..8614
    Translation

    Target ORF information:

    RefSeq Version XM_002936819.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936819.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCATC
    GACAAGGAGA ACGTGGAACT GTCTCCCACC ACAGGCCATT CAAACAGCAG TAGAACACGC
    CACGGTTCAG CAAGCCAAGT GCAAAAGCAG AGAAGTGCTG GCAGCTTCAA ACGTCACAGC
    ATTAAAAAGA TTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52431
    Clone ID Related Accession (Same CDS sequence) XM_018091610.2
    Accession Version XM_018091610.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CAGTGTGGTT
    CAGTGCTGTC TGTGTTTAGG AACAGCTCTA GCAACAGTCC CCAAGAGAGA ATATGTCAAC
    TGGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947099.1
    CDS257..8605
    Translation

    Target ORF information:

    RefSeq Version XM_018091610.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091610.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGCTTCT
    CTGCCTTGCT CTAAATCAGC CGTTTTCATG CAGCCACTCC CTCATCAAGG CAGTGTGGTT
    CAGTGCTGTC TGTGTTTAGG AACAGCTCTA GCAACAGTCC CCAAGAGAGA ATATGTCAAC
    TGGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52432
    Clone ID Related Accession (Same CDS sequence) XM_012957569.3
    Accession Version XM_012957569.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTTGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGGCATCGAC
    AAGGAGAACG TGGAACTGTC TCCCACCACA GGCCATTCAA ACAGCAGTAG AACACGCCAC
    GGTTCAGCAA GCCAAGTGCA AAAGCAGAGA AGTGCTGGCA GCTTCAAACG TCACAGCATT
    AAAAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813023.1
    CDS257..8551
    Translation

    Target ORF information:

    RefSeq Version XM_012957569.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957569.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGTTGT GAGTCAGAGA
    TTTCCACAAA ATAGCATTGG TGCAGTGGGA AGTGCAATGT TCCTCAGGTT CATAAATCCA
    GCCATTGTGT CACCATACGA AGCTGGAATC TTAGATAAGA AGCCACCACC ACGGCTTGAG
    CGTGGTTTGA AGTTAATGTC AAAGATTTTA CAAAGCATTG CTAATCATGT TCTGTTCACT
    AAAGAGGAAC ACATGCGTCC TTTTAATGAA TTTGTCAAAA GTAACTTTGA TGCTGCCAGG
    AGGTTCTTCT TTGACATAGC CAACAATTGC CCTCCTAGCG ACACGGTAAA TCACAGCCTC
    TCGTTTATAA CTGATGGGAA TGTACTCGCC TTACATCGGT TGCTCTGGAA CAACCAGGAA
    AAGATTGGAC AGTATCTATC CAGTAACAGG GACCACAAAG CTGTAGGAAG AAGACCTTTT
    GACAAAATGG CAACTCTTCT TGCGTACCTT GGACCTCCCG AACACAAACC TGTAGCTGAT
    ACACATTGGT CAAGTCTTAA CTTAACAAGC TCCAAGTTTG AAGAGTTTAT GACAAGGCAT
    CAGGTACATG AAAAAGAAGA ATTTAAAGCA TTAAAAACCC TAAATATATT TTACCAAGCA
    GGGACATCAA GAGCTGGTAA TCCAGTTTTC TACTATATTG CTAGAAGGTT CAAGACTGGT
    CAGATCAATG GTGACTTACT GATTTACCAC GTCTTGTTGA CTCTAAAGCC ATACTATGCC
    AAGCCATATG AAATAGTGGT AGATCTCACC CATGCTGGGC CCAGCAATCG CTTCAAGACA
    GACTTCCTTT CCAAATGGTT TGTGGTCTTT CCTGGTTTCG CTTATGAAAA TGTGACTGCA
    GTTTATATTT ACAACTGCAA CTCTTGGGTC AGGGAATACA CTAAATACCA TGAAAGGCTG
    TTAACCGGCC TAAAAGGCAG CAAAAGGCTA ATTTTCATTG ATCCATCTGG AAAGTTAACA
    GAGTATATTG AACATGAACA GCAAAAACTC CCAGCAGCCA CATTGGCATT GGAAGAAGAC
    CTAAAGTTGT TCCATAATGC ACTCAAATTA GCCCACAAAG ATACCAAGGT CTCCATAAAA
    GTTGGTTCTA CAGCTGTTCA GGTAACGTCA GCAGAGAGAA CTCGAGTCCT CGGGCAGTCA
    GTCTTTTTGA ATGACATTTA TTACGCATCT GAGATTGAGG AGATTTGTTT GGTGGATGAA
    AACCAGTTTA CTTTAACTAT CGCCAACCAA GGAACCCCTC TCACTTTCAT GCACCAGGAG
    TGTGAAGCCA TCGTCCAATC TATCATTCAC ATACGGACTC GATGGGAACT GTCACAACCA
    GATTCCATTC CTCAGCACAC TAAAATCCGT CCAAAGGATG TACCCGGAAC ACTACTAAAT
    ATTGCTTTAT TAAATCTGGG AAGTTCAGAC CCAAGTCTAC GGTCTGCTGC ATATAACCTT
    CTGTGTGCCT TAACATGTAC CTTTAATTTA AAGATTGAAG GGCAGTTACT AGAGACTTCG
    GGTCTGTGCA TTCCTGCCAA CAATACTCTC TTCATAGTTT CCATTAGTAA GACACTGGCA
    GCTAATGAGC CACATCTCAC TCTTGAGTTT TTAGAAGAGT GCATCTCTGG CTTCAGCAAA
    TCCAGTATCG AACTGAAGCA TCTGTGTCTT GAATACATGA CGCCTTGGCT GCTAAACCTT
    GTTCGGTTCT GTAAACTAAC AGATGATGCT AAACGTCAGC GTGTTAGTGC TATTTTGGAC
    AAACTTATAA CCATGACTAT AAATGAGAAA CAAATGTATC CATCCATACA AGCGAAAATA
    TGGGGCAGTC TTGGCCAGAT CACTGATCTC CTTGATGTTG TCCTGGACAG CTTTATCAAA
    ACCAGTGCAA CGGGTGGCTT GGGTTCCATT AAGGCTGAGG TTATGGCCGA TACAGCTGTT
    GCGCTTGCTT CGGGCAATGT GAAACTGGTT TCAAGCAAGG TTATTGGGAG AATGTGTAAA
    ATAATAGATA AGACCTGCCT CTCCCCAACA CCCACCCTTG AGCAGCATCT TATGTGGGAT
    GACATTGCCA TTCTTGCCCG GTACATGCTG ATGCTGTCTT TTAACAACTC TTTGGATGTG
    GCCGCCCATC TTCCCTACCT CTTCCATGTT GTCACTCTGT TAGTGGCAAC TGGCCCGCTG
    TCTCTTCGAG CTTCCACCCA TGGGCTTGTC ATCAATATCA TTCACTCCTT GTGCACTTGC
    TCTCAGCTGA ATTTCAGTGA GGAAACTAAA CAGGTATTAA GGCTAAGCCT GACTGAATTC
    TCACTGCCCA AGTTTTACCT GTTGTTTGGA ATAAGCAAGG TTAAATCTGC AGCTGTGATA
    GCCTTCAGAT CGAGTTATCG GGATCGATCA TTCTCACCTG GCTCTTATGA GAGGGAAACA
    TTTGCTTTGA CCTCATTGGA AACTGTTACA GAAGCCTTAT TGGAAATTAT GGAGGCTTGC
    ATGAGAGATA TCCCAACTTG CAAGTGGCTG GACCAGTGGA CAGAACTAGC TCAGAAGTTT
    GCCTTTCAGT ATAACCCATC ACTACAGCCA CGAGCTCTTG TAGTTTTTGG GTGCATTAGC
    AAACGGGTTT CTCATGGCCA AATCAAACAG ATCATACGAA TACTTAGCAA GGGGCTTGAA
    AGCTGCTTGA AAGGTCCTGA CAATTACAAC AGCCAAGTTT TAATTGAAGC CACCGTGATA
    GCACTAACGA AACTGCAGCC TCTCCTGAAT AAGGACTCTC CAATGCACAA AGCTCTCTTC
    TGGGTGGCTA TGGCAGTATT GCAGCTGGAT GAAGTTAATC TCTACTCAGC AGGAACAGCT
    CTCCTTGAAC AAAATTTGCA CACTTTAGAT AGCCTTAGAG TGTTCAATGA CAAAAGTCCA
    GAAGAAGTTT TTATGGAAAT AAGAAATCCA TTGGAGGAAT GGCATTGTAA ACAAATGGAC
    CATTTTGTTG GGCTCAACTT TAACTCTAAT TTTAATTTTG CACTAGTCGG ACATTTACTC
    AAAGGTTATC GACACCCATC TCCTACAACT GTGGCAAGGA CAATACGAAT TCTTCACACC
    CTCTTAGCAC TTGTCAACAA ACACAGAAAT TGTGATAAGT TTGAAGTGAA CACTGAGAGT
    GTTGCATACC TGGCAGCTTT GCTCACTGTT TCTGAAGAGG TGAGGAGCCG ATGCAGCCTG
    AAACACAGAA AATCTCTTTT ACTGACAGAT GTTTCAATGG AGAATGTTCC TATGGATACA
    TATCCAGTGC ATCATAATGA CCCCACATGC AGAACATTGA AGGAAAACCA GCCCTGGTCA
    TCACCACAAG CATCTGGTAT GCACCTAGCT GCTAGCTACC CAACAGTGGG GCAGGTTAGC
    CCACGAACCA GGAAGTCCAT GAGCCTCGAC ATGGGGCAAC CATCACAAGC AAACACTAAA
    AAGCTTCTTG GCACAAGGAA AAGCTTTGAC CACTTGATAT CTGACACTAA GGCTCCTAAA
    AGACAGGAGA TGGAGTCTGG CATCACTACA CCACCTAAGA TGAGGAGAGT TGCAGAGCAT
    GATTATGATA TTGACACACC AAGAATATCA TCCCCGCAAC AGCACCCTCA CCTCCGGAAA
    GTATCTGTGT CTGAGTCCAC TGTACTTTTG GATGAGGAGG TCCTGACGGA TCCAAAAACT
    CAAGCTTTGC TCCTCACTGT TCTTGCTACA CTTGTAAAAT ACACTACAGA TGAGTTTGAT
    CAACGCATCC TGTATGAGTA TTTAGCAGAA GCCAGTGTGG TTTTTCCTAA AGTATTCCCT
    GTGGTACATA ATTTGTTGGA TTCTAAGATA AGCACACTTC TATCTCTGTG CCAAGACCCA
    AATTTATTAA ATCCAATTCA TGGGATTGTT CAGAGTGTGG TGTACCATGA AGAGTCCCCT
    CCACAGTATC AGCCATCATA TTTACAAAGT TTTGGTTTTA ATGGTTTGTG GAGGTTTGCT
    GGACCTTTCT CCAAGCAAAC GCAAATCCCT GACTACGCAG AGCTGATAGT AAAATTTCTT
    GATGCCTTGA TTGATAAATA TTTGCCAGTA ATTGATGAAG AAGCCAGTGA AGAATCTCTC
    CTGACTCCTA CGTCTCCTTA CCCTCCTGCT GTGCAGAGTC AATTGAGTAT TACTGCTAAC
    CTTAATCTTT CCAACTCAAT GACCTCCCTT GCAACTTCCC AGCATTCCCC AGGCATCGAC
    AAGGAGAACG TGGAACTGTC TCCCACCACA GGCCATTCAA ACAGCAGTAG AACACGCCAC
    GGTTCAGCAA GCCAAGTGCA AAAGCAGAGA AGTGCTGGCA GCTTCAAACG TCACAGCATT
    AAAAAGATTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52433
    Clone ID Related Accession (Same CDS sequence) XM_018091613.2
    Accession Version XM_018091613.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCAGT
    GTGGTTCAGT GCTGTCTGTG TTTAGGAACA GCTCTAGCAA CAGTCCCCAA GAGAGAATAT
    GTCAACTGGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947102.1
    CDS257..8551
    Translation

    Target ORF information:

    RefSeq Version XM_018091613.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neurofibromin 1 (nf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091613.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCTC ACAAGCCAGT GGAGTGGGTG CAGGCGGTGG TAAACCGGTT CGATGAGCAG 
    CTTCCAATTA AAGCGAGTCA GCAGAATACC CACACCAAAG TCAGCATGGA GCACAACAAA
    GAGTGTCTCA TAAACATTTC AAAGTACAAA TTTTCTCTGG TCATAAGTGG ACTTACGAAC
    ATCTTAAAAA ATGTAAACCA CATGAGGATA TCTGGAGAGA CTTCAGAAAA AAATTTATAT
    TTATCTCAGT TGATTATTTT GGATACACTG GATAAATGTC TTGCAGGGCA ACCAAAGGAT
    TCTATGCGGT TAGATGAAAC AATGCTGGTT AAACAGTTAC TGCCAGAGAT CTGCCATTTT
    ATTCACACAT ACAGAGAAGG CAATCAGTAT GCAGCCGAAC TCCGAATGTC TGCTTCGGGG
    GTACTGTTCT CACTTAGCTG TAATAACTTT AATGCCGTCT TCAGCCGTAT TTCTACAAGG
    CTTCAGGAAC TGACAGTTTG CTCAGAGGAC AATGCTGATG TTCACGATAT AGACTTGATA
    CAATATATCA ATGTGGACTG TTCAAAACTT AAACGACTTT TACAGGAAAC AGTCTTTAAA
    TTTAAGTCTC TGAAGAAAGT TGCTCAGTTG GCAGTTATTA ACAGTCTCGA AAAGGCCTTT
    TGGAATTGGG TTGAAAATTA TCCAGATGAA TTTACAAAGT TGTACCAACG ACCACAGACT
    GATATGGCAG ACTGTGCAGA AAAGCTTTTT GACTTGGTGG ACAGCTTTGC CGAGAGCAAC
    AAGCGCAAAG CAGCAGTCTG GCCACTGCAG ATAATTTTGT TGGTTCTGTG TCCTGAAATT
    ATTCAGGATA TTTCCAAGGA TGTTGTAGAA GAAAGTAAAA TGAACAAGAA ACTATTCCTT
    GAAAACCTCA GAAAAGCTTT AGCTGGACAT GGAGGAACAA AGTCACTCAC AGAAACTGCA
    GCCATTGCTA GTGTCAAACT GTGCAAAGCC TCGACATACA TCAACTGGGA AGATAATTCT
    GTCATATTTC ACCTTGTGCA GTCTTTAGTC ATTGACTTAA AGAATTTGCT GTTTAATCCA
    AGCAAACCTT TCTCCAGAGG AGCAGTGAGC CAGAATGCTG ATTTGGATCT TATGATTGAC
    TGCCTGGTTT CTTGTTTTCG AATCAACCCT CACAACAACC AGCACTTTAA AACCTGTTTG
    GCGCAGTCAT CACCAACAAC ATTTCATTAT GTGCTTGTAA ATTCCCTGCA TAGAATAATT
    ACAAATTCTG CATTGGATTG GTGGCCTAAG ATTGATGCTG TGTATTGCTA CTCAGGAGAA
    CTGCGCAGCA TGTTTTCTGA AACTCTCAAA CTTGTTATGC AGCAGACAGC AAGCCGCCTG
    ACGCCGAGTC TAACTTTTAA GGAAAAAATG CCATACCTGA AATTTAAGGA CAGGTTAGCT
    GACCCAGAAA CCAAGAGTTA CAAGTACTTG CTGCTGTCTG TGGTAAAGCT CATTCATGGA
    GATCCAAAGC TGCTCTTATA TAATCCTGGA AAAGCAGTTT CTGATACACA AAGCAGTACT
    GCAGAATTAA TTAATGGACT GGTACAGCTG GTGCCTCAGT CAAATATGCC AGAAATTGCA
    CAGGAAGCTA TGGAGGCTCT GTTGGTTCTT CATCAGTCAG ACAGCATTGA GCTTTGGAAT
    CCAGAGGCTC CAATAGAAAC ATTCTGGGAA ATAAGCTCCC AAATGCTTTA TTCCATCTGT
    ACCAAGCTAA CTGGTCACCA GTTGCTTAAC AGCACAGAAA TTTTAAAATG GCTGCGAGAA
    ATCCTGATAT GTAGGAATAA ATTTCTTCTT AAATATAAGG AAAGTGCTGC TGCGGGAAGT
    GGAATCCCAA TATGCCGTCA AGCCCAGACA AAATTAGAAG TTGCACTTTA CATGTTCCTC
    TGGAGTCCTG ACACTGAGGC AGTTCTTGTT GCAATGTCCT GTTTTCGAAG TTTGTGTGAA
    GAGGCAGACA TCCGGTGTGG GGTTGATGAA GTATCTGTTC ATAATTTTTT GCCAAATTAC
    AACACATTTA TGGAGTTTGC ATCAGTAAGC AACATGGTGT CGACTGGGAG AGCAGCTCTG
    CAGAAGCGAG TTATGGCCTT ATTAAGAAGA ATAGAACACC CTACTGCAGG AAACACGGAG
    GCTTGGGAAG ATACACATGC AAAATGGGAT CATGCCACAA GACTGATTCT AAACTACCCC
    AAAACTAAAT TGGAGGATGG GCAGGCCCCA GAAAGCCTTC ATAAAACAAT TGTGAAAAGG
    AGAATGTCTC ATGTAAGTGG AGGGGGATCT ATAGACCTGT CTGACACAGA TTGTCTGCAG
    GAATGGATCA ACATGACTGG TTTCCTTTCT GCTCTTGGAG GAGTCTGCCT TCAACATCGA
    GGCAATGCTG GACTAGTTAC TTACAGTCCA CCCATGGGTC CAGTTAATGA ACGCAAAGGT
    TCTGTAATAT CAATGGTTTC CACTGAAGGA AATGCAGAAA CGCCTGTCAG CAAATTTCTT
    GATCGCTTAC TGTCTCTGAT GGTGTGCAAC CATGAAAAAG TGGGGATACA AATTCGAAAT
    AACATTAAGG ATTTAGTTGG TTTGGAGTTA AGCCCGGCTC TCTATCCAAT GTTATTTAAT
    AAAATGAAGA ACAACATAAG CAAATTTTTT GACTCTCAAG GACAGGTTCT CCTAACGGAT
    ACAAACACCC AGTTTGTCGA GCAGACAATT ACCATACTGA AGAACTTGCT TGATAATCAC
    ACAGAAGGCA GTTCAGAGCA TTTGGGCCAA GCAAGTCTTG AAACAATGAT GCTAAATTTA
    GTAAGGTACG TCCGGGTGCT AGGCAACTTG GTCCATGCCA TCCAGATTAA AACAAAGCTC
    TGTCAGTTGG TGGAAGTTAT GATGGAAAGA AGAGATGACC TCTCATTCTG TCAAGAGATG
    AAATTTAGGA ACAAAATGGT GGAGTACTTA ACTGACTGGG TAATGGGAAC ATCCAATCAG
    GCAGCGGATG AAGATGTAAA ATGCCTGACA AGAGACTTAG ATCAGGCCAG CATGGAAGCA
    GTTGTATCCC TACTCGCAGG TCTGCCTCTG CAGCCAGAGG AAGGGGATGG GGTGGAGTTA
    ATGGAGGCAA AATCTCAGCT TTTCCTGAAG TACTTCACGT TGTTCATGAA CTTGTTAAAT
    GACTGCAGCG AGGCTGAAGA TGATGGAACC CAGACCGGTG GTCGAAAAAG GGGGATGTCT
    AGGAGACTGG CTTCCTTAAG GCACTGTACT GTCCTGGCCA TGTCTAATTT GCTTAATGCT
    AATGTGGATA GTGGCCTTAT GCATTCAATA GGGCTTGGCT ATCACAAGGA TCTGCAGACA
    AGAGCGACAT TTATGGAGGT TCTAACAAAA ATACTACAGC AAGGAACAGA ATTTGATACA
    CTTGCTGAAA CTGTTCTGGC TGATCGTTTT GAAAGACTAG TAGAATTGGT GACCATGATG
    GGAGATCAAG GCGAATTACC TATTGCAATG GCTTTAGCCA ATGTTGTTCC ATGCTCACAG
    TGGGATGAGC TTGCTCGAGT TCTTGTGACA CTTTTCGATT CTCGTCACCT ACTCTACCAG
    CTTTTATGGA ATATGTTTTC TAAGGAAGTT GAACTGGCAG ATTCCATGCA GACACTGTTC
    AGAGGAAACA GCTTGGCTAG TAAAATAATG ACATTTTGCT TTAAGGTCTA TGGTGCCACA
    TATCTACAAA AGCTTCTGGA ACCTTTACTA AGGACTTTCA TTACATCACC TGAGTGGCAA
    TATGTCAGTT TTGAGGTTGA CTCAACAAGG CTTGAGGGAA ATGAACTCTT GGAAGAAAAT
    CAACGTCGCC TCCTACAGAT GACTGAGAAA TTCTTTCATG CCATTATTAA CTCCACAAAT
    GAGTTCCCAA CACAGCTTCG TAGTGTGTGC CATTGTCTGT ACCAGGCAAC TTGCCACTCC
    CTACTGAGTA AAGCTACCGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT TGTGAGTCAG
    AGATTTCCAC AAAATAGCAT TGGTGCAGTG GGAAGTGCAA TGTTCCTCAG GTTCATAAAT
    CCAGCCATTG TGTCACCATA CGAAGCTGGA ATCTTAGATA AGAAGCCACC ACCACGGCTT
    GAGCGTGGTT TGAAGTTAAT GTCAAAGATT TTACAAAGCA TTGCTAATCA TGTTCTGTTC
    ACTAAAGAGG AACACATGCG TCCTTTTAAT GAATTTGTCA AAAGTAACTT TGATGCTGCC
    AGGAGGTTCT TCTTTGACAT AGCCAACAAT TGCCCTCCTA GCGACACGGT AAATCACAGC
    CTCTCGTTTA TAACTGATGG GAATGTACTC GCCTTACATC GGTTGCTCTG GAACAACCAG
    GAAAAGATTG GACAGTATCT ATCCAGTAAC AGGGACCACA AAGCTGTAGG AAGAAGACCT
    TTTGACAAAA TGGCAACTCT TCTTGCGTAC CTTGGACCTC CCGAACACAA ACCTGTAGCT
    GATACACATT GGTCAAGTCT TAACTTAACA AGCTCCAAGT TTGAAGAGTT TATGACAAGG
    CATCAGGTAC ATGAAAAAGA AGAATTTAAA GCATTAAAAA CCCTAAATAT ATTTTACCAA
    GCAGGGACAT CAAGAGCTGG TAATCCAGTT TTCTACTATA TTGCTAGAAG GTTCAAGACT
    GGTCAGATCA ATGGTGACTT ACTGATTTAC CACGTCTTGT TGACTCTAAA GCCATACTAT
    GCCAAGCCAT ATGAAATAGT GGTAGATCTC ACCCATGCTG GGCCCAGCAA TCGCTTCAAG
    ACAGACTTCC TTTCCAAATG GTTTGTGGTC TTTCCTGGTT TCGCTTATGA AAATGTGACT
    GCAGTTTATA TTTACAACTG CAACTCTTGG GTCAGGGAAT ACACTAAATA CCATGAAAGG
    CTGTTAACCG GCCTAAAAGG CAGCAAAAGG CTAATTTTCA TTGATCCATC TGGAAAGTTA
    ACAGAGTATA TTGAACATGA ACAGCAAAAA CTCCCAGCAG CCACATTGGC ATTGGAAGAA
    GACCTAAAGT TGTTCCATAA TGCACTCAAA TTAGCCCACA AAGATACCAA GGTCTCCATA
    AAAGTTGGTT CTACAGCTGT TCAGGTAACG TCAGCAGAGA GAACTCGAGT CCTCGGGCAG
    TCAGTCTTTT TGAATGACAT TTATTACGCA TCTGAGATTG AGGAGATTTG TTTGGTGGAT
    GAAAACCAGT TTACTTTAAC TATCGCCAAC CAAGGAACCC CTCTCACTTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA ATCTATCATT CACATACGGA CTCGATGGGA ACTGTCACAA
    CCAGATTCCA TTCCTCAGCA CACTAAAATC CGTCCAAAGG ATGTACCCGG AACACTACTA
    AATATTGCTT TATTAAATCT GGGAAGTTCA GACCCAAGTC TACGGTCTGC TGCATATAAC
    CTTCTGTGTG CCTTAACATG TACCTTTAAT TTAAAGATTG AAGGGCAGTT ACTAGAGACT
    TCGGGTCTGT GCATTCCTGC CAACAATACT CTCTTCATAG TTTCCATTAG TAAGACACTG
    GCAGCTAATG AGCCACATCT CACTCTTGAG TTTTTAGAAG AGTGCATCTC TGGCTTCAGC
    AAATCCAGTA TCGAACTGAA GCATCTGTGT CTTGAATACA TGACGCCTTG GCTGCTAAAC
    CTTGTTCGGT TCTGTAAACT AACAGATGAT GCTAAACGTC AGCGTGTTAG TGCTATTTTG
    GACAAACTTA TAACCATGAC TATAAATGAG AAACAAATGT ATCCATCCAT ACAAGCGAAA
    ATATGGGGCA GTCTTGGCCA GATCACTGAT CTCCTTGATG TTGTCCTGGA CAGCTTTATC
    AAAACCAGTG CAACGGGTGG CTTGGGTTCC ATTAAGGCTG AGGTTATGGC CGATACAGCT
    GTTGCGCTTG CTTCGGGCAA TGTGAAACTG GTTTCAAGCA AGGTTATTGG GAGAATGTGT
    AAAATAATAG ATAAGACCTG CCTCTCCCCA ACACCCACCC TTGAGCAGCA TCTTATGTGG
    GATGACATTG CCATTCTTGC CCGGTACATG CTGATGCTGT CTTTTAACAA CTCTTTGGAT
    GTGGCCGCCC ATCTTCCCTA CCTCTTCCAT GTTGTCACTC TGTTAGTGGC AACTGGCCCG
    CTGTCTCTTC GAGCTTCCAC CCATGGGCTT GTCATCAATA TCATTCACTC CTTGTGCACT
    TGCTCTCAGC TGAATTTCAG TGAGGAAACT AAACAGGTAT TAAGGCTAAG CCTGACTGAA
    TTCTCACTGC CCAAGTTTTA CCTGTTGTTT GGAATAAGCA AGGTTAAATC TGCAGCTGTG
    ATAGCCTTCA GATCGAGTTA TCGGGATCGA TCATTCTCAC CTGGCTCTTA TGAGAGGGAA
    ACATTTGCTT TGACCTCATT GGAAACTGTT ACAGAAGCCT TATTGGAAAT TATGGAGGCT
    TGCATGAGAG ATATCCCAAC TTGCAAGTGG CTGGACCAGT GGACAGAACT AGCTCAGAAG
    TTTGCCTTTC AGTATAACCC ATCACTACAG CCACGAGCTC TTGTAGTTTT TGGGTGCATT
    AGCAAACGGG TTTCTCATGG CCAAATCAAA CAGATCATAC GAATACTTAG CAAGGGGCTT
    GAAAGCTGCT TGAAAGGTCC TGACAATTAC AACAGCCAAG TTTTAATTGA AGCCACCGTG
    ATAGCACTAA CGAAACTGCA GCCTCTCCTG AATAAGGACT CTCCAATGCA CAAAGCTCTC
    TTCTGGGTGG CTATGGCAGT ATTGCAGCTG GATGAAGTTA ATCTCTACTC AGCAGGAACA
    GCTCTCCTTG AACAAAATTT GCACACTTTA GATAGCCTTA GAGTGTTCAA TGACAAAAGT
    CCAGAAGAAG TTTTTATGGA AATAAGAAAT CCATTGGAGG AATGGCATTG TAAACAAATG
    GACCATTTTG TTGGGCTCAA CTTTAACTCT AATTTTAATT TTGCACTAGT CGGACATTTA
    CTCAAAGGTT ATCGACACCC ATCTCCTACA ACTGTGGCAA GGACAATACG AATTCTTCAC
    ACCCTCTTAG CACTTGTCAA CAAACACAGA AATTGTGATA AGTTTGAAGT GAACACTGAG
    AGTGTTGCAT ACCTGGCAGC TTTGCTCACT GTTTCTGAAG AGGTGAGGAG CCGATGCAGC
    CTGAAACACA GAAAATCTCT TTTACTGACA GATGTTTCAA TGGAGAATGT TCCTATGGAT
    ACATATCCAG TGCATCATAA TGACCCCACA TGCAGAACAT TGAAGGAAAA CCAGCCCTGG
    TCATCACCAC AAGCATCTGG TATGCACCTA GCTGCTAGCT ACCCAACAGT GGGGCAGGTT
    AGCCCACGAA CCAGGAAGTC CATGAGCCTC GACATGGGGC AACCATCACA AGCAAACACT
    AAAAAGCTTC TTGGCACAAG GAAAAGCTTT GACCACTTGA TATCTGACAC TAAGGCTCCT
    AAAAGACAGG AGATGGAGTC TGGCATCACT ACACCACCTA AGATGAGGAG AGTTGCAGAG
    CATGATTATG ATATTGACAC ACCAAGAATA TCATCCCCGC AACAGCACCC TCACCTCCGG
    AAAGTATCTG TGTCTGAGTC CACTGTACTT TTGGATGAGG AGGTCCTGAC GGATCCAAAA
    ACTCAAGCTT TGCTCCTCAC TGTTCTTGCT ACACTTGTAA AATACACTAC AGATGAGTTT
    GATCAACGCA TCCTGTATGA GTATTTAGCA GAAGCCAGTG TGGTTTTTCC TAAAGTATTC
    CCTGTGGTAC ATAATTTGTT GGATTCTAAG ATAAGCACAC TTCTATCTCT GTGCCAAGAC
    CCAAATTTAT TAAATCCAAT TCATGGGATT GTTCAGAGTG TGGTGTACCA TGAAGAGTCC
    CCTCCACAGT ATCAGCCATC ATATTTACAA AGTTTTGGTT TTAATGGTTT GTGGAGGTTT
    GCTGGACCTT TCTCCAAGCA AACGCAAATC CCTGACTACG CAGAGCTGAT AGTAAAATTT
    CTTGATGCCT TGATTGATAA ATATTTGCCA GTAATTGATG AAGAAGCCAG TGAAGAATCT
    CTCCTGACTC CTACGTCTCC TTACCCTCCT GCTGTGCAGA GTCAATTGAG TATTACTGCT
    AACCTTAATC TTTCCAACTC AATGACCTCC CTTGCAACTT CCCAGCATTC CCCAGGCAGT
    GTGGTTCAGT GCTGTCTGTG TTTAGGAACA GCTCTAGCAA CAGTCCCCAA GAGAGAATAT
    GTCAACTGGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.