vars2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following vars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa64480 XM_002940950.5
Latest version!
Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa64480 XM_004917033.4
Latest version!
Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa14617 XM_004917033.3
Latest version!
Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa14617 XM_002940950.4
Latest version!
Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa64480
    Clone ID Related Accession (Same CDS sequence) XM_004917033.4 , XM_002940950.5
    Accession Version XM_004917033.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product valine--tRNA ligase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCACCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAGGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAGTTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG ACAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AAAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTCGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGTTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACACCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917090.2
    CDS150..3251
    Translation

    Target ORF information:

    RefSeq Version XM_004917033.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917033.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCACCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAGGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAGTTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG ACAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AAAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTCGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGTTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACACCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64480
    Clone ID Related Accession (Same CDS sequence) XM_004917033.4 , XM_002940950.5
    Accession Version XM_002940950.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product valine--tRNA ligase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940950.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCACCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAGGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAGTTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG ACAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AAAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTCGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGTTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACACCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940996.3
    CDS147..3248
    Translation

    Target ORF information:

    RefSeq Version XM_002940950.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940950.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCACCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAGGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAGTTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG ACAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AAAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTCGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGTTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACACCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14617
    Clone ID Related Accession (Same CDS sequence) XM_004917033.3 , XM_002940950.4
    Accession Version XM_004917033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product valine--tRNA ligase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCTCCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAAGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAATTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG GCAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AGAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTTGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGCTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACTCCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917090.1
    CDS155..3256
    Translation

    Target ORF information:

    RefSeq Version XM_004917033.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCTCCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAAGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAATTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG GCAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AGAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTTGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGCTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACTCCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14617
    Clone ID Related Accession (Same CDS sequence) XM_004917033.3 , XM_002940950.4
    Accession Version XM_002940950.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product valine--tRNA ligase, mitochondrial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940950.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCTCCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAAGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAATTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG GCAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AGAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTTGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGCTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACTCCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940996.2
    CDS152..3253
    Translation

    Target ORF information:

    RefSeq Version XM_002940950.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis valyl-tRNA synthetase 2, mitochondrial (vars2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940950.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCAACGCC TCTTTTCTAA CCCCAGCCAA AATGTGGTGT CCACATTGTC CCGTAGGGGA 
    GTTGCTGGAA GTAGAGAAAG AGCCAGACGT GCTGAGCGTC AACAACGAAA ACAGAATTTG
    TTGGATGAGG TTGCTGAACA GGTACCCCAT GTCAAAGGCC AAAACTGGAA AGAGAAGATA
    GTGCTAGAAT ATGAAGCAGT GACGGAGCCA GGAAAAAAGA AAGATATCTC AGTTCCTTTA
    CCACATTCAT ACAGTCCTCG TTATGTTGAA GCTGCTTGGT ATCCGTGGTG GGTAAGACAG
    GGTTTTTTCA AGCCAGAGTA CCAGAGTCAG CAGCCTCACA ATGGATCAGA GACCTACTCA
    CTTTGCATAC CTCCTCCAAA TGTCACAGGC TCCCTTCACA TTGGACATGC ACTTACTGTA
    GCCATTGAGG ATTCACTTGT TCGATGGAAA AGGATGCTGG GCCACAAGGT GCTTTGGGTC
    CCTGGGTCAG ATCATGCTGG AATTGCCACC CAGGCTGTGG TTGAAAAGAA ACTGTTGAAG
    GAAAAGGGTC TCCTACGCCA TGATTTTAGT AGAGAGGAAT TTTTAAAAGA AGTTTGGAAG
    TGGAAAGAAG AGAAAGGTGA CATAATTTAC CATCAGCTAA GATCCCTAGG TGCCTCTCTT
    GACTGGGATA GATGCTGCTT TACAATGGAC AAGATGTTCT CTCGTGCTGT ATCTGAAGCA
    TTTGTCCGCC TGCATAATGA TGGTTTAGTG TATCGGCACC TGCGTCTGGT GAATTGGTCC
    TGTGCCCTGC GCTCTGCAAT ATCTGACATA GAGGTAGACA GTCGGCAGTT GACTGGAAAG
    ACATATATTT CTGTCCCAGG TTATGAAAGG AAAATCCCCT TTGGACTTCT ATTCAGCTTT
    GCCTATGTTA CTGAAGGAGG AGATGATGAG ATCATTGTAT CTACAACTCG ACCAGAAACG
    ATGTTGGGGG ATACTGCAGT GGCTGTTCAT CCTAATGACC CAAGATATTT GAGATTCCAT
    GGAACAAGAC TTCACCATCC TTTCACAGGG CAGCTGATAC CTGTTGTAAC TCATGATTTT
    GTGAACCCAG ACTTTGGAAC TGGAGCAGTG AAGGTTACTC CTGCCCACAG CCTGGCAGAT
    TTTGAGATGG CACAAAGCCT GGATTTGCCA GTGGTATCAG TGATTGGAGA GGATGGCTTA
    ATGACAGAGA ACAGTGGAGA ATGGATTCAG GGTGTGAAAA GATTTGATGC CAGGGAGCAG
    ATACTAAGTG CTCTGAAAGA AAAAGGTCTT TACAGAGGTG ACTATGAGCA TGCCATGGTG
    CTTCCAGTAT GCAGCCGCTC TGGTGATGTA ATTGAGCAGC TGTTAAAGAG GCAGTGGTTT
    GTGAATTGCA AGAAGATGGC ACAAAAGGCT CTAGAGGCTG TAAACGTAGG GCAGCTGAAG
    ATTATCCCTT CTTTTCACCA GAAGAACTGG AACCACTGGC TCTCAAACAT CAGTGATTGG
    TGTGTATCAC GACAACTTTG GTGGGGACAT CAGATTCCAG CTTACCATGT GGCAGTGTCA
    GAGGCACAGT CAGACAAGGA TATACGTGAT GAGCAAGAAG AAATTTGGGT AGTGGCTCAT
    AATGAATCTG AAGCCAAAAA AAAAGCTTCC AGAAAACTAA GAAGGCCAGA AAAGGATCTT
    TCCGTGAAGA GGGATGAAGA TGTTCTGGAT ACATGGTTTT CATCTGCCCT TTTCCCATTT
    GCTATGCTGG GCTGGCCAGA AAATACAAGT GACCTGAAGG ATTTTTACCC AAACTCTCTC
    TTGGAGACTG GTAGTGATTT ACTTTTCTTC TGGGTGGCTC GAATGGTAAT GTTGGGAGAG
    CATCTAACTG GGAAGTTACC ATTCAAAGAG GTATTTCTGC ATTCTATGGT TCGAGATGCT
    CATGGAAGGA AAATGAGCAA ATCTTTAGGA AATGTGATTG ATCCATTAGA TGTGATAACT
    GGAATATCAC TAGAGGAATT GCAGAATAGG TTGCAAGATG GCAATTTAGA TCCTCGAGAG
    CTGAAAGTTG CAGAGGCTGG GTTGTTAAAG GCTTTTCCTC GTGGGATTCC TGAGTGTGGC
    ACAGATGCTT TGCGATTTGC CCTTTGTTCC TACAAGGTAC AAGGGGATGA TATAAATCTA
    GATGTGCCAT CTGTTCTTGC TGTCCGCCAT TTTTGTAATA AAATCTGGAA TGGGATTAAA
    TTTACCCTCT CTTCTCTTGG GGAAAGCTTC TCACCTATTC CTGTGGAAGA GTTCAAACCT
    CAGGGCCCCA TGGATCGCTG GTTACTAGAT CACTTGCGTC TTTTGACAGA AGAATGTGAG
    AGAAGGTTTC AGGCTTATGA ACTTCATGGA GCAACAGGAG CCATCCAGAC ATTTTGGGTG
    CAGAGCTACT GTGACTTCTA TGTGGAAGCA GTAAAGCCTA TCTTAAAAGG GCCACATGGC
    AGTGATGCAC AACAGACTTT GTACTGGGGA GCAGAGATTG TCCTTCGTCT TCTTGCCCCC
    TTTACTCCTT TTTTATGTGA AGAGCTGTGG CAGCGGCTTC CTTCAGCCTC TGTGAATTGT
    GCACCAAGCA TCTGTGTTGC CCAGTATCCC ACTCCTAGCC AGATGGCTCA TTGGAGGTAC
    CCAGAAGAAG TGCAACAGTT TGATTTTGTG CAGTCAGTCA TTAGAACTGT GCGCAGTCTG
    CGAAGCGACT ATCACATGAC TAAGGCACGA CCAGAGGTTC ATATTTTCTG CTCCATGGAA
    GAACATCTCA ATGCTCTTCA GTCATTTCAC TCACATTTGC CGACTCTGTC TCACTCTGGA
    CCCATCCAAG TCCATATGTT GCAAGATGCT TCTCAGTTCA GGGAGGACCC TGTTGGCTGG
    GCTACAGAAA AAGTGTCTGA GGGCTGTTAC CTTCGTGTTA AACTCCAGGG TTTACTAGAC
    CCCCACACAG AGCTTGCAAA ACTGCACAAA AAGCATCAGA ATCTTCAGCT TCAGTTTATA
    AACATGTCAA AATATGAGAA TGGCGATAAT GAGGATAAAT CAATTCAGCA GAAAATCTCC
    AAGGTTCAAT GTACAATGGA AACATTACAG AGAATGCTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.