col4a6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col4a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa65391 XM_012968993.3
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65392 XM_031892000.1
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65393 XM_031892001.1
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa14355 XM_012968993.2
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14356 XM_018096582.1
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14358 XM_018096583.1
Latest version!
Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa65391
    Clone ID Related Accession (Same CDS sequence) XM_012968993.3
    Accession Version XM_012968993.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTCGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG CTGCCAAGGT
    TTTCCTGGAA ATAAAGGTGT ACCTGGAGAC AGAGGCATTC CGGGCATAGC TGGGATGAAG
    GGACGCCCAG GAGCTCCAGG GGATGCAGGA CGTCCTGGTG AATTAGGTCT CCCTGGGCTA
    CCTGGAACAG GTGGCCGTGA AGGTCTTCCA GGGTTCCAAG GACTTAAAGG AGAGAAAGGC
    TTTCCTGGTG TGAGGGGATT TGCTGGAATG CCTGGTATTC CAGGACTTCC TGGAGTCAGT
    GGATATAAAG GAATTTGTGG CTCACCTGGA CAAGATGGAC AAAAAGGAAA TGATGGCATT
    AAGGGAGTAA AAGGTTTACC TGGCGATCGT GGTCCACCAG GTGCTGTCAT GCCACCACCA
    CCCGGCCTTC TTTCTAAAGG TGCTCAAGGA GAACAAGGAC CAAGTGGCCC TCCTGGATTT
    ACAGGACCAA GAGGTGATAA AGGCTTTCCT GGACTTCCAG GAATACCTGG GAACCCTGGT
    TTTCCAGGCA ACCCAGGCAG AGATAAAGGT TTTCCAGGTG ACCCAGGATT TCCCGGAGCA
    TTTGGAAGAC CAGGATTTCC TGGTCCAAAA GGAGAACCTG GAATAATTGG ATTTCCTGGA
    ATGCCTGGAC CAAATGGTGC TGACGGTATC CCGGGAGTTT CTGGCTTTCC TGGCAGTCCT
    GGCCTACCAG GACCCAAGGG AGAAAGAGGA GAGACTTTAG GACAACCTGG ACGTCCAGGA
    GTTAAAGGAG AGACTGGCAT ACCAGGAATT TCAGGTCTAA GAGGTTTTGA TGGCATGCCT
    GGAGATGTTG GGTCAATAGG AATATCTGGA AATCCTGGAC CAAAGGGTCC TCCAGGAGAG
    TTTGGACGGA TAGGACAACC TGGAAATCCG GGGCAACAGG GATATAGAGG AAACCCTGGA
    TTAGTGGGTA GTTCTGGATT ACCAGGTTCT CCAGGATCCC CAGGATACCC AGGGGCACCA
    GGTCTTAAAG GTTTTCCTGG ATCTCAAGGT ATTGGTGGCT TAAATGGCTT GGCTGGAAGT
    AAAGGGTCCC CTGGAACACC AGGGCTTAGT GAGGCTGGAC GACCAGGAAC CCCTGGATTT
    CCAGGGCCAA AAGGTGAAAG AGGTTCTCCC AGCACAATAT CGGGGCAACC AGGATTTCCT
    GGATCTAAAG GATTTCCTGG AGAAAGAGGT CTTCCTGGTG CTGTTGGCTT ACCTGGTCTT
    CCTGGACCAC CAGGCTTCTC ATTCCCCTCT GCCATCCCAG GGAGACCTGG TGACCCTGGC
    TTTCCTGGAC GAGATGGGGA AGCAGGACTT CCTGGCCTGC CAGGGCCACG TGGCCCACCT
    GGCCCAGGAT CTAATCAAGG GGATACAGGG GACTCAGGAT ACCCAGGCAT TCCTGGCAGG
    CCTGGCATAA AGGGTGAACC TGGAATTCCA GGAAACCCAG GAAGCCCTGG AGCTCCTGGA
    CTGAAAGGTC TAAGAGGTGA TCCAGGTTTC ATTGGTAGCC CAGGCAAGGA AGGTTCTGTA
    GGAGATCCTG GCTTTCCAGG ACTAAAGGGA GAAAGGGGCA ACCCAGGACT TCCTGGTCCC
    CAAGGACGAC CTGGCCCTCC CCCATCGATA GAGGATTACC AGATTCCTAT TGGATTACCA
    GGTCTGCCTG GAATTAATGG TTTTGATGGT GTTCTTGGGG AAATCGGATC ACCAGGACCA
    AATGGACCAC CAGGAGCAAA AGGATTTCCA GGCAGATCTG GGCAAATGGG AAGAAATGGG
    CCACCTGGTG CTCCTGGTCC TATTGGTGAT CCTGGCGAAA TTGGTATTCC TGGTACTTCT
    GGACTTGAAG GTTCTCCTGG TAAGCAGGGT GCAGTTGGCT TACCCGGAAT GCCCGGACGG
    AGTGTGGGTG TTGGATACAC ATTAGTAAAG CACAGCCAGT CTGAACAGAT TCCTCCATGC
    CCGCTTGGAA TGGCCAGATT GTGGGATGGC TATAGCTTGC TGTTCGTGGA AGGACAAGAA
    AAGGCTCACA ATCAAGACTT GGGTCTTGCT GGTTCCTGTT TGCCCCGATT CAGCACCATG
    CCTTTTATTT ACTGCAACAT TAATGAGGTC TGCTACTATG CTAGCAGGAA TGACAAATCT
    TACTGGCTCT CTACCACGGC CCCTATCCCC ATGATGCCTG TGGGCAGCAC TGAGATTAAA
    AACTACATTA GTCGCTGCTC AGTGTGTGAA GCTTCTTCCC AGGCTGTTGC TGTTCATAGC
    CAGGATAACA CAATCCCACA ATGCCCTGAT GGATGGAGGA GCCTCTGGAT TGGATACTCT
    TTCCTCATGC ACACAGCTGC TGGTGCTGAA GGTGGCGGTC AGTCCTTAGT ATCTCCAGGT
    TCTTGCCTTG AAGATTTTCG GGCTACCCCA TTTATTGAAT GCAGCGGAGC AAGAGGAACC
    TGCCATTACT TTGCCAACAA GTACAGCTTC TGGTTGACCA CTGTGGAAGA AACACGGCAG
    TTTGCTAGAC CGCCCACTTC TGAAACCTTG AAATCTGGGC AGTTGAGAAC ACGTGTGAGC
    CGCTGCCAGG TGTGCATGAA GAACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824447.1
    CDS247..5376
    Translation

    Target ORF information:

    RefSeq Version XM_012968993.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968993.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTCGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG CTGCCAAGGT
    TTTCCTGGAA ATAAAGGTGT ACCTGGAGAC AGAGGCATTC CGGGCATAGC TGGGATGAAG
    GGACGCCCAG GAGCTCCAGG GGATGCAGGA CGTCCTGGTG AATTAGGTCT CCCTGGGCTA
    CCTGGAACAG GTGGCCGTGA AGGTCTTCCA GGGTTCCAAG GACTTAAAGG AGAGAAAGGC
    TTTCCTGGTG TGAGGGGATT TGCTGGAATG CCTGGTATTC CAGGACTTCC TGGAGTCAGT
    GGATATAAAG GAATTTGTGG CTCACCTGGA CAAGATGGAC AAAAAGGAAA TGATGGCATT
    AAGGGAGTAA AAGGTTTACC TGGCGATCGT GGTCCACCAG GTGCTGTCAT GCCACCACCA
    CCCGGCCTTC TTTCTAAAGG TGCTCAAGGA GAACAAGGAC CAAGTGGCCC TCCTGGATTT
    ACAGGACCAA GAGGTGATAA AGGCTTTCCT GGACTTCCAG GAATACCTGG GAACCCTGGT
    TTTCCAGGCA ACCCAGGCAG AGATAAAGGT TTTCCAGGTG ACCCAGGATT TCCCGGAGCA
    TTTGGAAGAC CAGGATTTCC TGGTCCAAAA GGAGAACCTG GAATAATTGG ATTTCCTGGA
    ATGCCTGGAC CAAATGGTGC TGACGGTATC CCGGGAGTTT CTGGCTTTCC TGGCAGTCCT
    GGCCTACCAG GACCCAAGGG AGAAAGAGGA GAGACTTTAG GACAACCTGG ACGTCCAGGA
    GTTAAAGGAG AGACTGGCAT ACCAGGAATT TCAGGTCTAA GAGGTTTTGA TGGCATGCCT
    GGAGATGTTG GGTCAATAGG AATATCTGGA AATCCTGGAC CAAAGGGTCC TCCAGGAGAG
    TTTGGACGGA TAGGACAACC TGGAAATCCG GGGCAACAGG GATATAGAGG AAACCCTGGA
    TTAGTGGGTA GTTCTGGATT ACCAGGTTCT CCAGGATCCC CAGGATACCC AGGGGCACCA
    GGTCTTAAAG GTTTTCCTGG ATCTCAAGGT ATTGGTGGCT TAAATGGCTT GGCTGGAAGT
    AAAGGGTCCC CTGGAACACC AGGGCTTAGT GAGGCTGGAC GACCAGGAAC CCCTGGATTT
    CCAGGGCCAA AAGGTGAAAG AGGTTCTCCC AGCACAATAT CGGGGCAACC AGGATTTCCT
    GGATCTAAAG GATTTCCTGG AGAAAGAGGT CTTCCTGGTG CTGTTGGCTT ACCTGGTCTT
    CCTGGACCAC CAGGCTTCTC ATTCCCCTCT GCCATCCCAG GGAGACCTGG TGACCCTGGC
    TTTCCTGGAC GAGATGGGGA AGCAGGACTT CCTGGCCTGC CAGGGCCACG TGGCCCACCT
    GGCCCAGGAT CTAATCAAGG GGATACAGGG GACTCAGGAT ACCCAGGCAT TCCTGGCAGG
    CCTGGCATAA AGGGTGAACC TGGAATTCCA GGAAACCCAG GAAGCCCTGG AGCTCCTGGA
    CTGAAAGGTC TAAGAGGTGA TCCAGGTTTC ATTGGTAGCC CAGGCAAGGA AGGTTCTGTA
    GGAGATCCTG GCTTTCCAGG ACTAAAGGGA GAAAGGGGCA ACCCAGGACT TCCTGGTCCC
    CAAGGACGAC CTGGCCCTCC CCCATCGATA GAGGATTACC AGATTCCTAT TGGATTACCA
    GGTCTGCCTG GAATTAATGG TTTTGATGGT GTTCTTGGGG AAATCGGATC ACCAGGACCA
    AATGGACCAC CAGGAGCAAA AGGATTTCCA GGCAGATCTG GGCAAATGGG AAGAAATGGG
    CCACCTGGTG CTCCTGGTCC TATTGGTGAT CCTGGCGAAA TTGGTATTCC TGGTACTTCT
    GGACTTGAAG GTTCTCCTGG TAAGCAGGGT GCAGTTGGCT TACCCGGAAT GCCCGGACGG
    AGTGTGGGTG TTGGATACAC ATTAGTAAAG CACAGCCAGT CTGAACAGAT TCCTCCATGC
    CCGCTTGGAA TGGCCAGATT GTGGGATGGC TATAGCTTGC TGTTCGTGGA AGGACAAGAA
    AAGGCTCACA ATCAAGACTT GGGTCTTGCT GGTTCCTGTT TGCCCCGATT CAGCACCATG
    CCTTTTATTT ACTGCAACAT TAATGAGGTC TGCTACTATG CTAGCAGGAA TGACAAATCT
    TACTGGCTCT CTACCACGGC CCCTATCCCC ATGATGCCTG TGGGCAGCAC TGAGATTAAA
    AACTACATTA GTCGCTGCTC AGTGTGTGAA GCTTCTTCCC AGGCTGTTGC TGTTCATAGC
    CAGGATAACA CAATCCCACA ATGCCCTGAT GGATGGAGGA GCCTCTGGAT TGGATACTCT
    TTCCTCATGC ACACAGCTGC TGGTGCTGAA GGTGGCGGTC AGTCCTTAGT ATCTCCAGGT
    TCTTGCCTTG AAGATTTTCG GGCTACCCCA TTTATTGAAT GCAGCGGAGC AAGAGGAACC
    TGCCATTACT TTGCCAACAA GTACAGCTTC TGGTTGACCA CTGTGGAAGA AACACGGCAG
    TTTGCTAGAC CGCCCACTTC TGAAACCTTG AAATCTGGGC AGTTGAGAAC ACGTGTGAGC
    CGCTGCCAGG TGTGCATGAA GAACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65392
    Clone ID Related Accession (Same CDS sequence) XM_031892000.1
    Accession Version XM_031892000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTCGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG TTTTCCTGGA
    AATAAAGGTG TACCTGGAGA CAGAGGCATT CCGGGCATAG CTGGGATGAA GGGACGCCCA
    GGAGCTCCAG GGGATGCAGG ACGTCCTGGT GAATTAGGTC TCCCTGGGCT ACCTGGAACA
    GGTGGCCGTG AAGGTCTTCC AGGGTTCCAA GGACTTAAAG GAGAGAAAGG CTTTCCTGGT
    GTGAGGGGAT TTGCTGGAAT GCCTGGTATT CCAGGACTTC CTGGAGTCAG TGGATATAAA
    GGAATTTGTG GCTCACCTGG ACAAGATGGA CAAAAAGGAA ATGATGGCAT TAAGGGAGTA
    AAAGGTTTAC CTGGCGATCG TGGTCCACCA GGTGCTGTCA TGCCACCACC ACCCGGCCTT
    CTTTCTAAAG GTGCTCAAGG AGAACAAGGA CCAAGTGGCC CTCCTGGATT TACAGGACCA
    AGAGGTGATA AAGGCTTTCC TGGACTTCCA GGAATACCTG GGAACCCTGG TTTTCCAGGC
    AACCCAGGCA GAGATAAAGG TTTTCCAGGT GACCCAGGAT TTCCCGGAGC ATTTGGAAGA
    CCAGGATTTC CTGGTCCAAA AGGAGAACCT GGAATAATTG GATTTCCTGG AATGCCTGGA
    CCAAATGGTG CTGACGGTAT CCCGGGAGTT TCTGGCTTTC CTGGCAGTCC TGGCCTACCA
    GGACCCAAGG GAGAAAGAGG AGAGACTTTA GGACAACCTG GACGTCCAGG AGTTAAAGGA
    GAGACTGGCA TACCAGGAAT TTCAGGTCTA AGAGGTTTTG ATGGCATGCC TGGAGATGTT
    GGGTCAATAG GAATATCTGG AAATCCTGGA CCAAAGGGTC CTCCAGGAGA GTTTGGACGG
    ATAGGACAAC CTGGAAATCC GGGGCAACAG GGATATAGAG GAAACCCTGG ATTAGTGGGT
    AGTTCTGGAT TACCAGGTTC TCCAGGATCC CCAGGATACC CAGGGGCACC AGGTCTTAAA
    GGTTTTCCTG GATCTCAAGG TATTGGTGGC TTAAATGGCT TGGCTGGAAG TAAAGGGTCC
    CCTGGAACAC CAGGGCTTAG TGAGGCTGGA CGACCAGGAA CCCCTGGATT TCCAGGGCCA
    AAAGGTGAAA GAGGTTCTCC CAGCACAATA TCGGGGCAAC CAGGATTTCC TGGATCTAAA
    GGATTTCCTG GAGAAAGAGG TCTTCCTGGT GCTGTTGGCT TACCTGGTCT TCCTGGACCA
    CCAGGCTTCT CATTCCCCTC TGCCATCCCA GGGAGACCTG GTGACCCTGG CTTTCCTGGA
    CGAGATGGGG AAGCAGGACT TCCTGGCCTG CCAGGGCCAC GTGGCCCACC TGGCCCAGGA
    TCTAATCAAG GGGATACAGG GGACTCAGGA TACCCAGGCA TTCCTGGCAG GCCTGGCATA
    AAGGGTGAAC CTGGAATTCC AGGAAACCCA GGAAGCCCTG GAGCTCCTGG ACTGAAAGGT
    CTAAGAGGTG ATCCAGGTTT CATTGGTAGC CCAGGCAAGG AAGGTTCTGT AGGAGATCCT
    GGCTTTCCAG GACTAAAGGG AGAAAGGGGC AACCCAGGAC TTCCTGGTCC CCAAGGACGA
    CCTGGCCCTC CCCCATCGAT AGAGGATTAC CAGATTCCTA TTGGATTACC AGGTCTGCCT
    GGAATTAATG GTTTTGATGG TGTTCTTGGG GAAATCGGAT CACCAGGACC AAATGGACCA
    CCAGGAGCAA AAGGATTTCC AGGCAGATCT GGGCAAATGG GAAGAAATGG GCCACCTGGT
    GCTCCTGGTC CTATTGGTGA TCCTGGCGAA ATTGGTATTC CTGGTACTTC TGGACTTGAA
    GGTTCTCCTG GTAAGCAGGG TGCAGTTGGC TTACCCGGAA TGCCCGGACG GAGTGTGGGT
    GTTGGATACA CATTAGTAAA GCACAGCCAG TCTGAACAGA TTCCTCCATG CCCGCTTGGA
    ATGGCCAGAT TGTGGGATGG CTATAGCTTG CTGTTCGTGG AAGGACAAGA AAAGGCTCAC
    AATCAAGACT TGGGTCTTGC TGGTTCCTGT TTGCCCCGAT TCAGCACCAT GCCTTTTATT
    TACTGCAACA TTAATGAGGT CTGCTACTAT GCTAGCAGGA ATGACAAATC TTACTGGCTC
    TCTACCACGG CCCCTATCCC CATGATGCCT GTGGGCAGCA CTGAGATTAA AAACTACATT
    AGTCGCTGCT CAGTGTGTGA AGCTTCTTCC CAGGCTGTTG CTGTTCATAG CCAGGATAAC
    ACAATCCCAC AATGCCCTGA TGGATGGAGG AGCCTCTGGA TTGGATACTC TTTCCTCATG
    CACACAGCTG CTGGTGCTGA AGGTGGCGGT CAGTCCTTAG TATCTCCAGG TTCTTGCCTT
    GAAGATTTTC GGGCTACCCC ATTTATTGAA TGCAGCGGAG CAAGAGGAAC CTGCCATTAC
    TTTGCCAACA AGTACAGCTT CTGGTTGACC ACTGTGGAAG AAACACGGCA GTTTGCTAGA
    CCGCCCACTT CTGAAACCTT GAAATCTGGG CAGTTGAGAA CACGTGTGAG CCGCTGCCAG
    GTGTGCATGA AGAACTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747860.1
    CDS247..5367
    Translation

    Target ORF information:

    RefSeq Version XM_031892000.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTCGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG TTTTCCTGGA
    AATAAAGGTG TACCTGGAGA CAGAGGCATT CCGGGCATAG CTGGGATGAA GGGACGCCCA
    GGAGCTCCAG GGGATGCAGG ACGTCCTGGT GAATTAGGTC TCCCTGGGCT ACCTGGAACA
    GGTGGCCGTG AAGGTCTTCC AGGGTTCCAA GGACTTAAAG GAGAGAAAGG CTTTCCTGGT
    GTGAGGGGAT TTGCTGGAAT GCCTGGTATT CCAGGACTTC CTGGAGTCAG TGGATATAAA
    GGAATTTGTG GCTCACCTGG ACAAGATGGA CAAAAAGGAA ATGATGGCAT TAAGGGAGTA
    AAAGGTTTAC CTGGCGATCG TGGTCCACCA GGTGCTGTCA TGCCACCACC ACCCGGCCTT
    CTTTCTAAAG GTGCTCAAGG AGAACAAGGA CCAAGTGGCC CTCCTGGATT TACAGGACCA
    AGAGGTGATA AAGGCTTTCC TGGACTTCCA GGAATACCTG GGAACCCTGG TTTTCCAGGC
    AACCCAGGCA GAGATAAAGG TTTTCCAGGT GACCCAGGAT TTCCCGGAGC ATTTGGAAGA
    CCAGGATTTC CTGGTCCAAA AGGAGAACCT GGAATAATTG GATTTCCTGG AATGCCTGGA
    CCAAATGGTG CTGACGGTAT CCCGGGAGTT TCTGGCTTTC CTGGCAGTCC TGGCCTACCA
    GGACCCAAGG GAGAAAGAGG AGAGACTTTA GGACAACCTG GACGTCCAGG AGTTAAAGGA
    GAGACTGGCA TACCAGGAAT TTCAGGTCTA AGAGGTTTTG ATGGCATGCC TGGAGATGTT
    GGGTCAATAG GAATATCTGG AAATCCTGGA CCAAAGGGTC CTCCAGGAGA GTTTGGACGG
    ATAGGACAAC CTGGAAATCC GGGGCAACAG GGATATAGAG GAAACCCTGG ATTAGTGGGT
    AGTTCTGGAT TACCAGGTTC TCCAGGATCC CCAGGATACC CAGGGGCACC AGGTCTTAAA
    GGTTTTCCTG GATCTCAAGG TATTGGTGGC TTAAATGGCT TGGCTGGAAG TAAAGGGTCC
    CCTGGAACAC CAGGGCTTAG TGAGGCTGGA CGACCAGGAA CCCCTGGATT TCCAGGGCCA
    AAAGGTGAAA GAGGTTCTCC CAGCACAATA TCGGGGCAAC CAGGATTTCC TGGATCTAAA
    GGATTTCCTG GAGAAAGAGG TCTTCCTGGT GCTGTTGGCT TACCTGGTCT TCCTGGACCA
    CCAGGCTTCT CATTCCCCTC TGCCATCCCA GGGAGACCTG GTGACCCTGG CTTTCCTGGA
    CGAGATGGGG AAGCAGGACT TCCTGGCCTG CCAGGGCCAC GTGGCCCACC TGGCCCAGGA
    TCTAATCAAG GGGATACAGG GGACTCAGGA TACCCAGGCA TTCCTGGCAG GCCTGGCATA
    AAGGGTGAAC CTGGAATTCC AGGAAACCCA GGAAGCCCTG GAGCTCCTGG ACTGAAAGGT
    CTAAGAGGTG ATCCAGGTTT CATTGGTAGC CCAGGCAAGG AAGGTTCTGT AGGAGATCCT
    GGCTTTCCAG GACTAAAGGG AGAAAGGGGC AACCCAGGAC TTCCTGGTCC CCAAGGACGA
    CCTGGCCCTC CCCCATCGAT AGAGGATTAC CAGATTCCTA TTGGATTACC AGGTCTGCCT
    GGAATTAATG GTTTTGATGG TGTTCTTGGG GAAATCGGAT CACCAGGACC AAATGGACCA
    CCAGGAGCAA AAGGATTTCC AGGCAGATCT GGGCAAATGG GAAGAAATGG GCCACCTGGT
    GCTCCTGGTC CTATTGGTGA TCCTGGCGAA ATTGGTATTC CTGGTACTTC TGGACTTGAA
    GGTTCTCCTG GTAAGCAGGG TGCAGTTGGC TTACCCGGAA TGCCCGGACG GAGTGTGGGT
    GTTGGATACA CATTAGTAAA GCACAGCCAG TCTGAACAGA TTCCTCCATG CCCGCTTGGA
    ATGGCCAGAT TGTGGGATGG CTATAGCTTG CTGTTCGTGG AAGGACAAGA AAAGGCTCAC
    AATCAAGACT TGGGTCTTGC TGGTTCCTGT TTGCCCCGAT TCAGCACCAT GCCTTTTATT
    TACTGCAACA TTAATGAGGT CTGCTACTAT GCTAGCAGGA ATGACAAATC TTACTGGCTC
    TCTACCACGG CCCCTATCCC CATGATGCCT GTGGGCAGCA CTGAGATTAA AAACTACATT
    AGTCGCTGCT CAGTGTGTGA AGCTTCTTCC CAGGCTGTTG CTGTTCATAG CCAGGATAAC
    ACAATCCCAC AATGCCCTGA TGGATGGAGG AGCCTCTGGA TTGGATACTC TTTCCTCATG
    CACACAGCTG CTGGTGCTGA AGGTGGCGGT CAGTCCTTAG TATCTCCAGG TTCTTGCCTT
    GAAGATTTTC GGGCTACCCC ATTTATTGAA TGCAGCGGAG CAAGAGGAAC CTGCCATTAC
    TTTGCCAACA AGTACAGCTT CTGGTTGACC ACTGTGGAAG AAACACGGCA GTTTGCTAGA
    CCGCCCACTT CTGAAACCTT GAAATCTGGG CAGTTGAGAA CACGTGTGAG CCGCTGCCAG
    GTGTGCATGA AGAACTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65393
    Clone ID Related Accession (Same CDS sequence) XM_031892001.1
    Accession Version XM_031892001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGAACAACAT TACCCTGTGT TATTGCTGGA
    GAACCAGGCC CACCAGGAAT CCGTGGCACA CCAGGATTTC CAGGTCGTAA AGGTAATATG
    GGATTCCCAG GAGTTCCAGG ACAACAGGGC TTTGATGGTG CTAAAGGACA ACCAGGCAAT
    CCAGGTTTTG GTGGAATTCC AGGGCCTCCT GGATTTCCTG GACCCCGTGG AGAAGAAGGT
    GCTCCAGGGT TCAATGGATT GCCAGGTAGT GAAGGACTTC CTGGAAAGTT CGGGATCCCA
    GGGTCCCCTG GTTTAAAAGG ATTGCGAGGA GAAGTATTGG GAGCAACACC TGGAGCTCCT
    GGTGAGCCAG GCCTACCTGG AATCAATGGA GACAAAGGCT TACCAGGAGA TGCTGGTTTA
    TCAGGACAGA GAGGTTTTGA TGGAAGGCCT GGACTGATGG GTGCTAAAGG AGAACGGGGT
    AACTCTGGTT TCCCTGGCAG CCCAGGTCCC AAAGGTCAAC CTGGCTTTGG AGGGTCAATG
    GGGATTAAAG GAAAACAGGG TCCTCCTGGA GATATTGGAT ACCCAGGCTC ACCAGGCTGC
    CAAGGTTTTC CTGGAAATAA AGGTGTACCT GGAGACAGAG GCATTCCGGG CATAGCTGGG
    ATGAAGGGAC GCCCAGGAGC TCCAGGGGAT GCAGGACGTC CTGGTGAATT AGGTCTCCCT
    GGGCTACCTG GAACAGGTGG CCGTGAAGGT CTTCCAGGGT TCCAAGGACT TAAAGGAGAG
    AAAGGCTTTC CTGGTGTGAG GGGATTTGCT GGAATGCCTG GTATTCCAGG ACTTCCTGGA
    GTCAGTGGAT ATAAAGGAAT TTGTGGCTCA CCTGGACAAG ATGGACAAAA AGGAAATGAT
    GGCATTAAGG GAGTAAAAGG TTTACCTGGC GATCGTGGTC CACCAGGTGC TGTCATGCCA
    CCACCACCCG GCCTTCTTTC TAAAGGTGCT CAAGGAGAAC AAGGACCAAG TGGCCCTCCT
    GGATTTACAG GACCAAGAGG TGATAAAGGC TTTCCTGGAC TTCCAGGAAT ACCTGGGAAC
    CCTGGTTTTC CAGGCAACCC AGGCAGAGAT AAAGGTTTTC CAGGTGACCC AGGATTTCCC
    GGAGCATTTG GAAGACCAGG ATTTCCTGGT CCAAAAGGAG AACCTGGAAT AATTGGATTT
    CCTGGAATGC CTGGACCAAA TGGTGCTGAC GGTATCCCGG GAGTTTCTGG CTTTCCTGGC
    AGTCCTGGCC TACCAGGACC CAAGGGAGAA AGAGGAGAGA CTTTAGGACA ACCTGGACGT
    CCAGGAGTTA AAGGAGAGAC TGGCATACCA GGAATTTCAG GTCTAAGAGG TTTTGATGGC
    ATGCCTGGAG ATGTTGGGTC AATAGGAATA TCTGGAAATC CTGGACCAAA GGGTCCTCCA
    GGAGAGTTTG GACGGATAGG ACAACCTGGA AATCCGGGGC AACAGGGATA TAGAGGAAAC
    CCTGGATTAG TGGGTAGTTC TGGATTACCA GGTTCTCCAG GATCCCCAGG ATACCCAGGG
    GCACCAGGTC TTAAAGGTTT TCCTGGATCT CAAGGTATTG GTGGCTTAAA TGGCTTGGCT
    GGAAGTAAAG GGTCCCCTGG AACACCAGGG CTTAGTGAGG CTGGACGACC AGGAACCCCT
    GGATTTCCAG GGCCAAAAGG TGAAAGAGGT TCTCCCAGCA CAATATCGGG GCAACCAGGA
    TTTCCTGGAT CTAAAGGATT TCCTGGAGAA AGAGGTCTTC CTGGTGCTGT TGGCTTACCT
    GGTCTTCCTG GACCACCAGG CTTCTCATTC CCCTCTGCCA TCCCAGGGAG ACCTGGTGAC
    CCTGGCTTTC CTGGACGAGA TGGGGAAGCA GGACTTCCTG GCCTGCCAGG GCCACGTGGC
    CCACCTGGCC CAGGATCTAA TCAAGGGGAT ACAGGGGACT CAGGATACCC AGGCATTCCT
    GGCAGGCCTG GCATAAAGGG TGAACCTGGA ATTCCAGGAA ACCCAGGAAG CCCTGGAGCT
    CCTGGACTGA AAGGTCTAAG AGGTGATCCA GGTTTCATTG GTAGCCCAGG CAAGGAAGGT
    TCTGTAGGAG ATCCTGGCTT TCCAGGACTA AAGGGAGAAA GGGGCAACCC AGGACTTCCT
    GGTCCCCAAG GACGACCTGG CCCTCCCCCA TCGATAGAGG ATTACCAGAT TCCTATTGGA
    TTACCAGGTC TGCCTGGAAT TAATGGTTTT GATGGTGTTC TTGGGGAAAT CGGATCACCA
    GGACCAAATG GACCACCAGG AGCAAAAGGA TTTCCAGGCA GATCTGGGCA AATGGGAAGA
    AATGGGCCAC CTGGTGCTCC TGGTCCTATT GGTGATCCTG GCGAAATTGG TATTCCTGGT
    ACTTCTGGAC TTGAAGGTTC TCCTGGTAAG CAGGGTGCAG TTGGCTTACC CGGAATGCCC
    GGACGGAGTG TGGGTGTTGG ATACACATTA GTAAAGCACA GCCAGTCTGA ACAGATTCCT
    CCATGCCCGC TTGGAATGGC CAGATTGTGG GATGGCTATA GCTTGCTGTT CGTGGAAGGA
    CAAGAAAAGG CTCACAATCA AGACTTGGGT CTTGCTGGTT CCTGTTTGCC CCGATTCAGC
    ACCATGCCTT TTATTTACTG CAACATTAAT GAGGTCTGCT ACTATGCTAG CAGGAATGAC
    AAATCTTACT GGCTCTCTAC CACGGCCCCT ATCCCCATGA TGCCTGTGGG CAGCACTGAG
    ATTAAAAACT ACATTAGTCG CTGCTCAGTG TGTGAAGCTT CTTCCCAGGC TGTTGCTGTT
    CATAGCCAGG ATAACACAAT CCCACAATGC CCTGATGGAT GGAGGAGCCT CTGGATTGGA
    TACTCTTTCC TCATGCACAC AGCTGCTGGT GCTGAAGGTG GCGGTCAGTC CTTAGTATCT
    CCAGGTTCTT GCCTTGAAGA TTTTCGGGCT ACCCCATTTA TTGAATGCAG CGGAGCAAGA
    GGAACCTGCC ATTACTTTGC CAACAAGTAC AGCTTCTGGT TGACCACTGT GGAAGAAACA
    CGGCAGTTTG CTAGACCGCC CACTTCTGAA ACCTTGAAAT CTGGGCAGTT GAGAACACGT
    GTGAGCCGCT GCCAGGTGTG CATGAAGAAC TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031747861.1
    CDS247..5322
    Translation

    Target ORF information:

    RefSeq Version XM_031892001.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGAACAACAT TACCCTGTGT TATTGCTGGA
    GAACCAGGCC CACCAGGAAT CCGTGGCACA CCAGGATTTC CAGGTCGTAA AGGTAATATG
    GGATTCCCAG GAGTTCCAGG ACAACAGGGC TTTGATGGTG CTAAAGGACA ACCAGGCAAT
    CCAGGTTTTG GTGGAATTCC AGGGCCTCCT GGATTTCCTG GACCCCGTGG AGAAGAAGGT
    GCTCCAGGGT TCAATGGATT GCCAGGTAGT GAAGGACTTC CTGGAAAGTT CGGGATCCCA
    GGGTCCCCTG GTTTAAAAGG ATTGCGAGGA GAAGTATTGG GAGCAACACC TGGAGCTCCT
    GGTGAGCCAG GCCTACCTGG AATCAATGGA GACAAAGGCT TACCAGGAGA TGCTGGTTTA
    TCAGGACAGA GAGGTTTTGA TGGAAGGCCT GGACTGATGG GTGCTAAAGG AGAACGGGGT
    AACTCTGGTT TCCCTGGCAG CCCAGGTCCC AAAGGTCAAC CTGGCTTTGG AGGGTCAATG
    GGGATTAAAG GAAAACAGGG TCCTCCTGGA GATATTGGAT ACCCAGGCTC ACCAGGCTGC
    CAAGGTTTTC CTGGAAATAA AGGTGTACCT GGAGACAGAG GCATTCCGGG CATAGCTGGG
    ATGAAGGGAC GCCCAGGAGC TCCAGGGGAT GCAGGACGTC CTGGTGAATT AGGTCTCCCT
    GGGCTACCTG GAACAGGTGG CCGTGAAGGT CTTCCAGGGT TCCAAGGACT TAAAGGAGAG
    AAAGGCTTTC CTGGTGTGAG GGGATTTGCT GGAATGCCTG GTATTCCAGG ACTTCCTGGA
    GTCAGTGGAT ATAAAGGAAT TTGTGGCTCA CCTGGACAAG ATGGACAAAA AGGAAATGAT
    GGCATTAAGG GAGTAAAAGG TTTACCTGGC GATCGTGGTC CACCAGGTGC TGTCATGCCA
    CCACCACCCG GCCTTCTTTC TAAAGGTGCT CAAGGAGAAC AAGGACCAAG TGGCCCTCCT
    GGATTTACAG GACCAAGAGG TGATAAAGGC TTTCCTGGAC TTCCAGGAAT ACCTGGGAAC
    CCTGGTTTTC CAGGCAACCC AGGCAGAGAT AAAGGTTTTC CAGGTGACCC AGGATTTCCC
    GGAGCATTTG GAAGACCAGG ATTTCCTGGT CCAAAAGGAG AACCTGGAAT AATTGGATTT
    CCTGGAATGC CTGGACCAAA TGGTGCTGAC GGTATCCCGG GAGTTTCTGG CTTTCCTGGC
    AGTCCTGGCC TACCAGGACC CAAGGGAGAA AGAGGAGAGA CTTTAGGACA ACCTGGACGT
    CCAGGAGTTA AAGGAGAGAC TGGCATACCA GGAATTTCAG GTCTAAGAGG TTTTGATGGC
    ATGCCTGGAG ATGTTGGGTC AATAGGAATA TCTGGAAATC CTGGACCAAA GGGTCCTCCA
    GGAGAGTTTG GACGGATAGG ACAACCTGGA AATCCGGGGC AACAGGGATA TAGAGGAAAC
    CCTGGATTAG TGGGTAGTTC TGGATTACCA GGTTCTCCAG GATCCCCAGG ATACCCAGGG
    GCACCAGGTC TTAAAGGTTT TCCTGGATCT CAAGGTATTG GTGGCTTAAA TGGCTTGGCT
    GGAAGTAAAG GGTCCCCTGG AACACCAGGG CTTAGTGAGG CTGGACGACC AGGAACCCCT
    GGATTTCCAG GGCCAAAAGG TGAAAGAGGT TCTCCCAGCA CAATATCGGG GCAACCAGGA
    TTTCCTGGAT CTAAAGGATT TCCTGGAGAA AGAGGTCTTC CTGGTGCTGT TGGCTTACCT
    GGTCTTCCTG GACCACCAGG CTTCTCATTC CCCTCTGCCA TCCCAGGGAG ACCTGGTGAC
    CCTGGCTTTC CTGGACGAGA TGGGGAAGCA GGACTTCCTG GCCTGCCAGG GCCACGTGGC
    CCACCTGGCC CAGGATCTAA TCAAGGGGAT ACAGGGGACT CAGGATACCC AGGCATTCCT
    GGCAGGCCTG GCATAAAGGG TGAACCTGGA ATTCCAGGAA ACCCAGGAAG CCCTGGAGCT
    CCTGGACTGA AAGGTCTAAG AGGTGATCCA GGTTTCATTG GTAGCCCAGG CAAGGAAGGT
    TCTGTAGGAG ATCCTGGCTT TCCAGGACTA AAGGGAGAAA GGGGCAACCC AGGACTTCCT
    GGTCCCCAAG GACGACCTGG CCCTCCCCCA TCGATAGAGG ATTACCAGAT TCCTATTGGA
    TTACCAGGTC TGCCTGGAAT TAATGGTTTT GATGGTGTTC TTGGGGAAAT CGGATCACCA
    GGACCAAATG GACCACCAGG AGCAAAAGGA TTTCCAGGCA GATCTGGGCA AATGGGAAGA
    AATGGGCCAC CTGGTGCTCC TGGTCCTATT GGTGATCCTG GCGAAATTGG TATTCCTGGT
    ACTTCTGGAC TTGAAGGTTC TCCTGGTAAG CAGGGTGCAG TTGGCTTACC CGGAATGCCC
    GGACGGAGTG TGGGTGTTGG ATACACATTA GTAAAGCACA GCCAGTCTGA ACAGATTCCT
    CCATGCCCGC TTGGAATGGC CAGATTGTGG GATGGCTATA GCTTGCTGTT CGTGGAAGGA
    CAAGAAAAGG CTCACAATCA AGACTTGGGT CTTGCTGGTT CCTGTTTGCC CCGATTCAGC
    ACCATGCCTT TTATTTACTG CAACATTAAT GAGGTCTGCT ACTATGCTAG CAGGAATGAC
    AAATCTTACT GGCTCTCTAC CACGGCCCCT ATCCCCATGA TGCCTGTGGG CAGCACTGAG
    ATTAAAAACT ACATTAGTCG CTGCTCAGTG TGTGAAGCTT CTTCCCAGGC TGTTGCTGTT
    CATAGCCAGG ATAACACAAT CCCACAATGC CCTGATGGAT GGAGGAGCCT CTGGATTGGA
    TACTCTTTCC TCATGCACAC AGCTGCTGGT GCTGAAGGTG GCGGTCAGTC CTTAGTATCT
    CCAGGTTCTT GCCTTGAAGA TTTTCGGGCT ACCCCATTTA TTGAATGCAG CGGAGCAAGA
    GGAACCTGCC ATTACTTTGC CAACAAGTAC AGCTTCTGGT TGACCACTGT GGAAGAAACA
    CGGCAGTTTG CTAGACCGCC CACTTCTGAA ACCTTGAAAT CTGGGCAGTT GAGAACACGT
    GTGAGCCGCT GCCAGGTGTG CATGAAGAAC TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14355
    Clone ID Related Accession (Same CDS sequence) XM_012968993.2
    Accession Version XM_012968993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTTGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG CTGCCAAGGT
    TTTCCTGGAA ATAAAGGTGT ACCTGGAGAC AGAGGCATTC CGGGCATAGC TGGGATGAAG
    GGACGCCCAG GAGCTCCAGG GGATGCAGGA CGTCCTGGTG AATTAGGTCT CCCTGGGCTA
    CCTGGAACAG GTGGCCGTGA AGGTCTTCCA GGGTTCCAAG GACTTAAAGG AGAGAAAGGC
    TTTCCTGGTG TGAGGGGATT TGCTGGAATG CCTGGTATTC CAGGACTTCC TGGAGTCAGT
    GGATATAAAG GAATTTGTGG CTCACCTGGA CAAGATGGAC AAAAAGGAAA TGATGGCATT
    AAGGGAGTAA AAGGTTTACC TGGCGATCGT GGTCCACCAG GTGCTGTCAT GCCACCACCA
    CCCGGCCTTC TTTCTAAAGG TGCTCAAGGA GAACAAGGAC CAAGTGGCCC TCCTGGATTT
    ACAGGACCAA GAGGTGATAA AGGCTTTCCT GGACTTCCAG GAATACCTGG GAACCCTGGT
    TTTCCAGGCA ACCCAGGCAG AGATAAAGGT TTTCCAGGTG ACCCAGGATT TCCCGGAGCA
    TTTGGAAGAC CAGGATTTCC TGGTCCAAAA GGAGAACCTG GAATAATTGG ATTTCCTGGA
    ATGCCTGGAC CAAATGGTGC TGACGGTATC CCGGGAGTTT CTGGCTTTCC TGGCAGTCCT
    GGCCTACCAG GACCCAAGGG AGAAAGAGGA GAGACTTTAG GACAACCTGG ACGTCCAGGA
    GTTAAAGGAG AGACTGGCAT ACCAGGAATT TCAGGTCTAA GAGGTTTTGA TGGCATGCCT
    GGAGATGTTG GGTCAATAGG AATATCTGGA AATCCTGGAC CAAAGGGTCC TCCAGGAGAG
    TTTGGACGGA TAGGACAACC TGGAAATCCG GGGCAACAGG GATATAGAGG AAACCCTGGA
    TTAGTGGGTA GTTCTGGATT ACCAGGTTCT CCAGGATCCC CAGGATACCC AGGGGCACCA
    GGTCTTAAAG GTTTTCCTGG ATCTCAAGGT ATTGGTGGCT TAAATGGCTT GGCTGGAAGT
    AAAGGGTCCC CTGGAACACC AGGGCTTAGT GAGGCTGGAC GACCAGGAAC CCCTGGATTT
    CCAGGGCCAA AAGGTGAAAG AGGTTCTCCC AGCACAATAT CGGGGCAACC AGGATTTCCT
    GGATCTAAAG GATTTCCTGG AGAAAGAGGT CTTCCTGGTG CTGTTGGCTT ACCTGGTCTT
    CCTGGACCAC CAGGCTTCTC ATTCCCCTCT GCCATCCCAG GGAGACCTGG TGACCCTGGC
    TTTCCTGGAC GAGATGGGGA AGCAGGACTT CCTGGCCTGC CAGGGCCACG TGGCCCACCT
    GGCCCAGGAT CTAATCAAGG GGATACAGGG GACTCAGGAT ACCCAGGCAT TCCTGGCAGG
    CCTGGCATAA AGGGTGAACC TGGAATTCCA GGAAACCCAG GAAGCCCTGG AGCTCCTGGA
    CTGAAAGGTC TAAGAGGTGA TCCAGGTTTC ATTGGTAGCC CAGGCAAGGA AGGTTCTGTA
    GGAGATCCTG GCTTTCCAGG ACTAAAGGGA GAAAGGGGCA ACCCAGGACT TCCTGGTCCC
    CAAGGACGAC CTGGCCCTCC CCCATCGATA GAGGATTACC AGATTCCTAT TGGATTACCA
    GGTCTGCCTG GAATTAATGG TTTTGATGGT GTTCTTGGGG AAATCGGATC ACCAGGACCA
    AATGGACCAC CAGGAGCAAA AGGATTTCCA GGCAGATCTG GGCAAATGGG AAGAAATGGG
    CCACCTGGTG CTCCTGGTCC TATTGGTGAT CCTGGCGAAA TTGGTATTCC TGGTACTTCT
    GGACTTGAAG GTTCTCCTGG TAAGCAGGGT GCAGTTGGCT TACCCGGAAT GCCCGGACGG
    AGTGTGGGTG TTGGATACAC ATTAGTAAAG CACAGCCAGT CTGAACAGAT TCCTCCATGC
    CCGCTTGGAA TGGCCAGATT GTGGGATGGC TATAGCTTGC TGTTCGTGGA AGGACAAGAA
    AAGGCTCACA ATCAAGACTT GGGTCTTGCT GGTTCCTGTT TGCCCCGATT CAGCACCATG
    CCTTTTATTT ACTGCAACAT TAATGAGGTC TGCTACTATG CTAGCAGGAA TGACAAATCT
    TACTGGCTCT CTACCACGGC CCCTATCCCC ATGATGCCTG TGGGCAGCAC TGAGATTAAA
    AACTACATTA GTCGCTGCTC AGTGTGTGAA GCTTCTTCCC AGGCTGTTGC TGTTCATAGC
    CAGGATAACA CAATCCCACA ATGCCCTGAT GGATGGAGGA GCCTCTGGAT TGGATACTCT
    TTCCTCATGC ACACAGCTGC TGGTGCTGAA GGTGGCGGTC AGTCCTTAGT ATCTCCAGGT
    TCTTGCCTTG AAGATTTTCG GGCTACCCCA TTTATTGAAT GCAGCGGAGC AAGAGGAACC
    TGCCATTACT TTGCCAACAA GTACAGCTTC TGGTTGACCA CTGTGGAAGA AACACGGCAG
    TTTGCTAGAC CGCCCACTTC TGAAACCTTG AAATCTGGGC AGTTGAGAAC ACGTGTGAGC
    CGCTGCCAGG TGTGCATGAA GAACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824447.1
    CDS241..5370
    Translation

    Target ORF information:

    RefSeq Version XM_012968993.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012968993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTTGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG CTGCCAAGGT
    TTTCCTGGAA ATAAAGGTGT ACCTGGAGAC AGAGGCATTC CGGGCATAGC TGGGATGAAG
    GGACGCCCAG GAGCTCCAGG GGATGCAGGA CGTCCTGGTG AATTAGGTCT CCCTGGGCTA
    CCTGGAACAG GTGGCCGTGA AGGTCTTCCA GGGTTCCAAG GACTTAAAGG AGAGAAAGGC
    TTTCCTGGTG TGAGGGGATT TGCTGGAATG CCTGGTATTC CAGGACTTCC TGGAGTCAGT
    GGATATAAAG GAATTTGTGG CTCACCTGGA CAAGATGGAC AAAAAGGAAA TGATGGCATT
    AAGGGAGTAA AAGGTTTACC TGGCGATCGT GGTCCACCAG GTGCTGTCAT GCCACCACCA
    CCCGGCCTTC TTTCTAAAGG TGCTCAAGGA GAACAAGGAC CAAGTGGCCC TCCTGGATTT
    ACAGGACCAA GAGGTGATAA AGGCTTTCCT GGACTTCCAG GAATACCTGG GAACCCTGGT
    TTTCCAGGCA ACCCAGGCAG AGATAAAGGT TTTCCAGGTG ACCCAGGATT TCCCGGAGCA
    TTTGGAAGAC CAGGATTTCC TGGTCCAAAA GGAGAACCTG GAATAATTGG ATTTCCTGGA
    ATGCCTGGAC CAAATGGTGC TGACGGTATC CCGGGAGTTT CTGGCTTTCC TGGCAGTCCT
    GGCCTACCAG GACCCAAGGG AGAAAGAGGA GAGACTTTAG GACAACCTGG ACGTCCAGGA
    GTTAAAGGAG AGACTGGCAT ACCAGGAATT TCAGGTCTAA GAGGTTTTGA TGGCATGCCT
    GGAGATGTTG GGTCAATAGG AATATCTGGA AATCCTGGAC CAAAGGGTCC TCCAGGAGAG
    TTTGGACGGA TAGGACAACC TGGAAATCCG GGGCAACAGG GATATAGAGG AAACCCTGGA
    TTAGTGGGTA GTTCTGGATT ACCAGGTTCT CCAGGATCCC CAGGATACCC AGGGGCACCA
    GGTCTTAAAG GTTTTCCTGG ATCTCAAGGT ATTGGTGGCT TAAATGGCTT GGCTGGAAGT
    AAAGGGTCCC CTGGAACACC AGGGCTTAGT GAGGCTGGAC GACCAGGAAC CCCTGGATTT
    CCAGGGCCAA AAGGTGAAAG AGGTTCTCCC AGCACAATAT CGGGGCAACC AGGATTTCCT
    GGATCTAAAG GATTTCCTGG AGAAAGAGGT CTTCCTGGTG CTGTTGGCTT ACCTGGTCTT
    CCTGGACCAC CAGGCTTCTC ATTCCCCTCT GCCATCCCAG GGAGACCTGG TGACCCTGGC
    TTTCCTGGAC GAGATGGGGA AGCAGGACTT CCTGGCCTGC CAGGGCCACG TGGCCCACCT
    GGCCCAGGAT CTAATCAAGG GGATACAGGG GACTCAGGAT ACCCAGGCAT TCCTGGCAGG
    CCTGGCATAA AGGGTGAACC TGGAATTCCA GGAAACCCAG GAAGCCCTGG AGCTCCTGGA
    CTGAAAGGTC TAAGAGGTGA TCCAGGTTTC ATTGGTAGCC CAGGCAAGGA AGGTTCTGTA
    GGAGATCCTG GCTTTCCAGG ACTAAAGGGA GAAAGGGGCA ACCCAGGACT TCCTGGTCCC
    CAAGGACGAC CTGGCCCTCC CCCATCGATA GAGGATTACC AGATTCCTAT TGGATTACCA
    GGTCTGCCTG GAATTAATGG TTTTGATGGT GTTCTTGGGG AAATCGGATC ACCAGGACCA
    AATGGACCAC CAGGAGCAAA AGGATTTCCA GGCAGATCTG GGCAAATGGG AAGAAATGGG
    CCACCTGGTG CTCCTGGTCC TATTGGTGAT CCTGGCGAAA TTGGTATTCC TGGTACTTCT
    GGACTTGAAG GTTCTCCTGG TAAGCAGGGT GCAGTTGGCT TACCCGGAAT GCCCGGACGG
    AGTGTGGGTG TTGGATACAC ATTAGTAAAG CACAGCCAGT CTGAACAGAT TCCTCCATGC
    CCGCTTGGAA TGGCCAGATT GTGGGATGGC TATAGCTTGC TGTTCGTGGA AGGACAAGAA
    AAGGCTCACA ATCAAGACTT GGGTCTTGCT GGTTCCTGTT TGCCCCGATT CAGCACCATG
    CCTTTTATTT ACTGCAACAT TAATGAGGTC TGCTACTATG CTAGCAGGAA TGACAAATCT
    TACTGGCTCT CTACCACGGC CCCTATCCCC ATGATGCCTG TGGGCAGCAC TGAGATTAAA
    AACTACATTA GTCGCTGCTC AGTGTGTGAA GCTTCTTCCC AGGCTGTTGC TGTTCATAGC
    CAGGATAACA CAATCCCACA ATGCCCTGAT GGATGGAGGA GCCTCTGGAT TGGATACTCT
    TTCCTCATGC ACACAGCTGC TGGTGCTGAA GGTGGCGGTC AGTCCTTAGT ATCTCCAGGT
    TCTTGCCTTG AAGATTTTCG GGCTACCCCA TTTATTGAAT GCAGCGGAGC AAGAGGAACC
    TGCCATTACT TTGCCAACAA GTACAGCTTC TGGTTGACCA CTGTGGAAGA AACACGGCAG
    TTTGCTAGAC CGCCCACTTC TGAAACCTTG AAATCTGGGC AGTTGAGAAC ACGTGTGAGC
    CGCTGCCAGG TGTGCATGAA GAACTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14356
    Clone ID Related Accession (Same CDS sequence) XM_018096582.1
    Accession Version XM_018096582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTTGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG TTTTCCTGGA
    AATAAAGGTG TACCTGGAGA CAGAGGCATT CCGGGCATAG CTGGGATGAA GGGACGCCCA
    GGAGCTCCAG GGGATGCAGG ACGTCCTGGT GAATTAGGTC TCCCTGGGCT ACCTGGAACA
    GGTGGCCGTG AAGGTCTTCC AGGGTTCCAA GGACTTAAAG GAGAGAAAGG CTTTCCTGGT
    GTGAGGGGAT TTGCTGGAAT GCCTGGTATT CCAGGACTTC CTGGAGTCAG TGGATATAAA
    GGAATTTGTG GCTCACCTGG ACAAGATGGA CAAAAAGGAA ATGATGGCAT TAAGGGAGTA
    AAAGGTTTAC CTGGCGATCG TGGTCCACCA GGTGCTGTCA TGCCACCACC ACCCGGCCTT
    CTTTCTAAAG GTGCTCAAGG AGAACAAGGA CCAAGTGGCC CTCCTGGATT TACAGGACCA
    AGAGGTGATA AAGGCTTTCC TGGACTTCCA GGAATACCTG GGAACCCTGG TTTTCCAGGC
    AACCCAGGCA GAGATAAAGG TTTTCCAGGT GACCCAGGAT TTCCCGGAGC ATTTGGAAGA
    CCAGGATTTC CTGGTCCAAA AGGAGAACCT GGAATAATTG GATTTCCTGG AATGCCTGGA
    CCAAATGGTG CTGACGGTAT CCCGGGAGTT TCTGGCTTTC CTGGCAGTCC TGGCCTACCA
    GGACCCAAGG GAGAAAGAGG AGAGACTTTA GGACAACCTG GACGTCCAGG AGTTAAAGGA
    GAGACTGGCA TACCAGGAAT TTCAGGTCTA AGAGGTTTTG ATGGCATGCC TGGAGATGTT
    GGGTCAATAG GAATATCTGG AAATCCTGGA CCAAAGGGTC CTCCAGGAGA GTTTGGACGG
    ATAGGACAAC CTGGAAATCC GGGGCAACAG GGATATAGAG GAAACCCTGG ATTAGTGGGT
    AGTTCTGGAT TACCAGGTTC TCCAGGATCC CCAGGATACC CAGGGGCACC AGGTCTTAAA
    GGTTTTCCTG GATCTCAAGG TATTGGTGGC TTAAATGGCT TGGCTGGAAG TAAAGGGTCC
    CCTGGAACAC CAGGGCTTAG TGAGGCTGGA CGACCAGGAA CCCCTGGATT TCCAGGGCCA
    AAAGGTGAAA GAGGTTCTCC CAGCACAATA TCGGGGCAAC CAGGATTTCC TGGATCTAAA
    GGATTTCCTG GAGAAAGAGG TCTTCCTGGT GCTGTTGGCT TACCTGGTCT TCCTGGACCA
    CCAGGCTTCT CATTCCCCTC TGCCATCCCA GGGAGACCTG GTGACCCTGG CTTTCCTGGA
    CGAGATGGGG AAGCAGGACT TCCTGGCCTG CCAGGGCCAC GTGGCCCACC TGGCCCAGGA
    TCTAATCAAG GGGATACAGG GGACTCAGGA TACCCAGGCA TTCCTGGCAG GCCTGGCATA
    AAGGGTGAAC CTGGAATTCC AGGAAACCCA GGAAGCCCTG GAGCTCCTGG ACTGAAAGGT
    CTAAGAGGTG ATCCAGGTTT CATTGGTAGC CCAGGCAAGG AAGGTTCTGT AGGAGATCCT
    GGCTTTCCAG GACTAAAGGG AGAAAGGGGC AACCCAGGAC TTCCTGGTCC CCAAGGACGA
    CCTGGCCCTC CCCCATCGAT AGAGGATTAC CAGATTCCTA TTGGATTACC AGGTCTGCCT
    GGAATTAATG GTTTTGATGG TGTTCTTGGG GAAATCGGAT CACCAGGACC AAATGGACCA
    CCAGGAGCAA AAGGATTTCC AGGCAGATCT GGGCAAATGG GAAGAAATGG GCCACCTGGT
    GCTCCTGGTC CTATTGGTGA TCCTGGCGAA ATTGGTATTC CTGGTACTTC TGGACTTGAA
    GGTTCTCCTG GTAAGCAGGG TGCAGTTGGC TTACCCGGAA TGCCCGGACG GAGTGTGGGT
    GTTGGATACA CATTAGTAAA GCACAGCCAG TCTGAACAGA TTCCTCCATG CCCGCTTGGA
    ATGGCCAGAT TGTGGGATGG CTATAGCTTG CTGTTCGTGG AAGGACAAGA AAAGGCTCAC
    AATCAAGACT TGGGTCTTGC TGGTTCCTGT TTGCCCCGAT TCAGCACCAT GCCTTTTATT
    TACTGCAACA TTAATGAGGT CTGCTACTAT GCTAGCAGGA ATGACAAATC TTACTGGCTC
    TCTACCACGG CCCCTATCCC CATGATGCCT GTGGGCAGCA CTGAGATTAA AAACTACATT
    AGTCGCTGCT CAGTGTGTGA AGCTTCTTCC CAGGCTGTTG CTGTTCATAG CCAGGATAAC
    ACAATCCCAC AATGCCCTGA TGGATGGAGG AGCCTCTGGA TTGGATACTC TTTCCTCATG
    CACACAGCTG CTGGTGCTGA AGGTGGCGGT CAGTCCTTAG TATCTCCAGG TTCTTGCCTT
    GAAGATTTTC GGGCTACCCC ATTTATTGAA TGCAGCGGAG CAAGAGGAAC CTGCCATTAC
    TTTGCCAACA AGTACAGCTT CTGGTTGACC ACTGTGGAAG AAACACGGCA GTTTGCTAGA
    CCGCCCACTT CTGAAACCTT GAAATCTGGG CAGTTGAGAA CACGTGTGAG CCGCTGCCAG
    GTGTGCATGA AGAACTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952071.1
    CDS237..5357
    Translation

    Target ORF information:

    RefSeq Version XM_018096582.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGTGACTGCT GCTGCGGGGC CAATGTTGGT
    AATGGAGACT TAGTCACAGA GGGAGGAACA ACATTACCCT GTGTTATTGC TGGAGAACCA
    GGCCCACCAG GAATCCGTGG CACACCAGGA TTTCCAGGTC GTAAAGGTAA TATGGGATTC
    CCAGGAGTTC CAGGACAACA GGGCTTTGAT GGTGCTAAAG GACAACCAGG CAATCCAGGT
    TTTGGTGGAA TTCCAGGGCC TCCTGGATTT CCTGGACCCC GTGGAGAAGA AGGTGCTCCA
    GGGTTCAATG GATTGCCAGG TAGTGAAGGA CTTCCTGGAA AGTTTGGGAT CCCAGGGTCC
    CCTGGTTTAA AAGGATTGCG AGGAGAAGTA TTGGGAGCAA CACCTGGAGC TCCTGGTGAG
    CCAGGCCTAC CTGGAATCAA TGGAGACAAA GGCTTACCAG GAGATGCTGG TTTATCAGGA
    CAGAGAGGTT TTGATGGAAG GCCTGGACTG ATGGGTGCTA AAGGAGAACG GGGTAACTCT
    GGTTTCCCTG GCAGCCCAGG TCCCAAAGGT CAACCTGGCT TTGGAGGGTC AATGGGGATT
    AAAGGAAAAC AGGGTCCTCC TGGAGATATT GGATACCCAG GCTCACCAGG TTTTCCTGGA
    AATAAAGGTG TACCTGGAGA CAGAGGCATT CCGGGCATAG CTGGGATGAA GGGACGCCCA
    GGAGCTCCAG GGGATGCAGG ACGTCCTGGT GAATTAGGTC TCCCTGGGCT ACCTGGAACA
    GGTGGCCGTG AAGGTCTTCC AGGGTTCCAA GGACTTAAAG GAGAGAAAGG CTTTCCTGGT
    GTGAGGGGAT TTGCTGGAAT GCCTGGTATT CCAGGACTTC CTGGAGTCAG TGGATATAAA
    GGAATTTGTG GCTCACCTGG ACAAGATGGA CAAAAAGGAA ATGATGGCAT TAAGGGAGTA
    AAAGGTTTAC CTGGCGATCG TGGTCCACCA GGTGCTGTCA TGCCACCACC ACCCGGCCTT
    CTTTCTAAAG GTGCTCAAGG AGAACAAGGA CCAAGTGGCC CTCCTGGATT TACAGGACCA
    AGAGGTGATA AAGGCTTTCC TGGACTTCCA GGAATACCTG GGAACCCTGG TTTTCCAGGC
    AACCCAGGCA GAGATAAAGG TTTTCCAGGT GACCCAGGAT TTCCCGGAGC ATTTGGAAGA
    CCAGGATTTC CTGGTCCAAA AGGAGAACCT GGAATAATTG GATTTCCTGG AATGCCTGGA
    CCAAATGGTG CTGACGGTAT CCCGGGAGTT TCTGGCTTTC CTGGCAGTCC TGGCCTACCA
    GGACCCAAGG GAGAAAGAGG AGAGACTTTA GGACAACCTG GACGTCCAGG AGTTAAAGGA
    GAGACTGGCA TACCAGGAAT TTCAGGTCTA AGAGGTTTTG ATGGCATGCC TGGAGATGTT
    GGGTCAATAG GAATATCTGG AAATCCTGGA CCAAAGGGTC CTCCAGGAGA GTTTGGACGG
    ATAGGACAAC CTGGAAATCC GGGGCAACAG GGATATAGAG GAAACCCTGG ATTAGTGGGT
    AGTTCTGGAT TACCAGGTTC TCCAGGATCC CCAGGATACC CAGGGGCACC AGGTCTTAAA
    GGTTTTCCTG GATCTCAAGG TATTGGTGGC TTAAATGGCT TGGCTGGAAG TAAAGGGTCC
    CCTGGAACAC CAGGGCTTAG TGAGGCTGGA CGACCAGGAA CCCCTGGATT TCCAGGGCCA
    AAAGGTGAAA GAGGTTCTCC CAGCACAATA TCGGGGCAAC CAGGATTTCC TGGATCTAAA
    GGATTTCCTG GAGAAAGAGG TCTTCCTGGT GCTGTTGGCT TACCTGGTCT TCCTGGACCA
    CCAGGCTTCT CATTCCCCTC TGCCATCCCA GGGAGACCTG GTGACCCTGG CTTTCCTGGA
    CGAGATGGGG AAGCAGGACT TCCTGGCCTG CCAGGGCCAC GTGGCCCACC TGGCCCAGGA
    TCTAATCAAG GGGATACAGG GGACTCAGGA TACCCAGGCA TTCCTGGCAG GCCTGGCATA
    AAGGGTGAAC CTGGAATTCC AGGAAACCCA GGAAGCCCTG GAGCTCCTGG ACTGAAAGGT
    CTAAGAGGTG ATCCAGGTTT CATTGGTAGC CCAGGCAAGG AAGGTTCTGT AGGAGATCCT
    GGCTTTCCAG GACTAAAGGG AGAAAGGGGC AACCCAGGAC TTCCTGGTCC CCAAGGACGA
    CCTGGCCCTC CCCCATCGAT AGAGGATTAC CAGATTCCTA TTGGATTACC AGGTCTGCCT
    GGAATTAATG GTTTTGATGG TGTTCTTGGG GAAATCGGAT CACCAGGACC AAATGGACCA
    CCAGGAGCAA AAGGATTTCC AGGCAGATCT GGGCAAATGG GAAGAAATGG GCCACCTGGT
    GCTCCTGGTC CTATTGGTGA TCCTGGCGAA ATTGGTATTC CTGGTACTTC TGGACTTGAA
    GGTTCTCCTG GTAAGCAGGG TGCAGTTGGC TTACCCGGAA TGCCCGGACG GAGTGTGGGT
    GTTGGATACA CATTAGTAAA GCACAGCCAG TCTGAACAGA TTCCTCCATG CCCGCTTGGA
    ATGGCCAGAT TGTGGGATGG CTATAGCTTG CTGTTCGTGG AAGGACAAGA AAAGGCTCAC
    AATCAAGACT TGGGTCTTGC TGGTTCCTGT TTGCCCCGAT TCAGCACCAT GCCTTTTATT
    TACTGCAACA TTAATGAGGT CTGCTACTAT GCTAGCAGGA ATGACAAATC TTACTGGCTC
    TCTACCACGG CCCCTATCCC CATGATGCCT GTGGGCAGCA CTGAGATTAA AAACTACATT
    AGTCGCTGCT CAGTGTGTGA AGCTTCTTCC CAGGCTGTTG CTGTTCATAG CCAGGATAAC
    ACAATCCCAC AATGCCCTGA TGGATGGAGG AGCCTCTGGA TTGGATACTC TTTCCTCATG
    CACACAGCTG CTGGTGCTGA AGGTGGCGGT CAGTCCTTAG TATCTCCAGG TTCTTGCCTT
    GAAGATTTTC GGGCTACCCC ATTTATTGAA TGCAGCGGAG CAAGAGGAAC CTGCCATTAC
    TTTGCCAACA AGTACAGCTT CTGGTTGACC ACTGTGGAAG AAACACGGCA GTTTGCTAGA
    CCGCCCACTT CTGAAACCTT GAAATCTGGG CAGTTGAGAA CACGTGTGAG CCGCTGCCAG
    GTGTGCATGA AGAACTTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14358
    Clone ID Related Accession (Same CDS sequence) XM_018096583.1
    Accession Version XM_018096583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGAACAACAT TACCCTGTGT TATTGCTGGA
    GAACCAGGCC CACCAGGAAT CCGTGGCACA CCAGGATTTC CAGGTCGTAA AGGTAATATG
    GGATTCCCAG GAGTTCCAGG ACAACAGGGC TTTGATGGTG CTAAAGGACA ACCAGGCAAT
    CCAGGTTTTG GTGGAATTCC AGGGCCTCCT GGATTTCCTG GACCCCGTGG AGAAGAAGGT
    GCTCCAGGGT TCAATGGATT GCCAGGTAGT GAAGGACTTC CTGGAAAGTT TGGGATCCCA
    GGGTCCCCTG GTTTAAAAGG ATTGCGAGGA GAAGTATTGG GAGCAACACC TGGAGCTCCT
    GGTGAGCCAG GCCTACCTGG AATCAATGGA GACAAAGGCT TACCAGGAGA TGCTGGTTTA
    TCAGGACAGA GAGGTTTTGA TGGAAGGCCT GGACTGATGG GTGCTAAAGG AGAACGGGGT
    AACTCTGGTT TCCCTGGCAG CCCAGGTCCC AAAGGTCAAC CTGGCTTTGG AGGGTCAATG
    GGGATTAAAG GAAAACAGGG TCCTCCTGGA GATATTGGAT ACCCAGGCTC ACCAGGCTGC
    CAAGGTTTTC CTGGAAATAA AGGTGTACCT GGAGACAGAG GCATTCCGGG CATAGCTGGG
    ATGAAGGGAC GCCCAGGAGC TCCAGGGGAT GCAGGACGTC CTGGTGAATT AGGTCTCCCT
    GGGCTACCTG GAACAGGTGG CCGTGAAGGT CTTCCAGGGT TCCAAGGACT TAAAGGAGAG
    AAAGGCTTTC CTGGTGTGAG GGGATTTGCT GGAATGCCTG GTATTCCAGG ACTTCCTGGA
    GTCAGTGGAT ATAAAGGAAT TTGTGGCTCA CCTGGACAAG ATGGACAAAA AGGAAATGAT
    GGCATTAAGG GAGTAAAAGG TTTACCTGGC GATCGTGGTC CACCAGGTGC TGTCATGCCA
    CCACCACCCG GCCTTCTTTC TAAAGGTGCT CAAGGAGAAC AAGGACCAAG TGGCCCTCCT
    GGATTTACAG GACCAAGAGG TGATAAAGGC TTTCCTGGAC TTCCAGGAAT ACCTGGGAAC
    CCTGGTTTTC CAGGCAACCC AGGCAGAGAT AAAGGTTTTC CAGGTGACCC AGGATTTCCC
    GGAGCATTTG GAAGACCAGG ATTTCCTGGT CCAAAAGGAG AACCTGGAAT AATTGGATTT
    CCTGGAATGC CTGGACCAAA TGGTGCTGAC GGTATCCCGG GAGTTTCTGG CTTTCCTGGC
    AGTCCTGGCC TACCAGGACC CAAGGGAGAA AGAGGAGAGA CTTTAGGACA ACCTGGACGT
    CCAGGAGTTA AAGGAGAGAC TGGCATACCA GGAATTTCAG GTCTAAGAGG TTTTGATGGC
    ATGCCTGGAG ATGTTGGGTC AATAGGAATA TCTGGAAATC CTGGACCAAA GGGTCCTCCA
    GGAGAGTTTG GACGGATAGG ACAACCTGGA AATCCGGGGC AACAGGGATA TAGAGGAAAC
    CCTGGATTAG TGGGTAGTTC TGGATTACCA GGTTCTCCAG GATCCCCAGG ATACCCAGGG
    GCACCAGGTC TTAAAGGTTT TCCTGGATCT CAAGGTATTG GTGGCTTAAA TGGCTTGGCT
    GGAAGTAAAG GGTCCCCTGG AACACCAGGG CTTAGTGAGG CTGGACGACC AGGAACCCCT
    GGATTTCCAG GGCCAAAAGG TGAAAGAGGT TCTCCCAGCA CAATATCGGG GCAACCAGGA
    TTTCCTGGAT CTAAAGGATT TCCTGGAGAA AGAGGTCTTC CTGGTGCTGT TGGCTTACCT
    GGTCTTCCTG GACCACCAGG CTTCTCATTC CCCTCTGCCA TCCCAGGGAG ACCTGGTGAC
    CCTGGCTTTC CTGGACGAGA TGGGGAAGCA GGACTTCCTG GCCTGCCAGG GCCACGTGGC
    CCACCTGGCC CAGGATCTAA TCAAGGGGAT ACAGGGGACT CAGGATACCC AGGCATTCCT
    GGCAGGCCTG GCATAAAGGG TGAACCTGGA ATTCCAGGAA ACCCAGGAAG CCCTGGAGCT
    CCTGGACTGA AAGGTCTAAG AGGTGATCCA GGTTTCATTG GTAGCCCAGG CAAGGAAGGT
    TCTGTAGGAG ATCCTGGCTT TCCAGGACTA AAGGGAGAAA GGGGCAACCC AGGACTTCCT
    GGTCCCCAAG GACGACCTGG CCCTCCCCCA TCGATAGAGG ATTACCAGAT TCCTATTGGA
    TTACCAGGTC TGCCTGGAAT TAATGGTTTT GATGGTGTTC TTGGGGAAAT CGGATCACCA
    GGACCAAATG GACCACCAGG AGCAAAAGGA TTTCCAGGCA GATCTGGGCA AATGGGAAGA
    AATGGGCCAC CTGGTGCTCC TGGTCCTATT GGTGATCCTG GCGAAATTGG TATTCCTGGT
    ACTTCTGGAC TTGAAGGTTC TCCTGGTAAG CAGGGTGCAG TTGGCTTACC CGGAATGCCC
    GGACGGAGTG TGGGTGTTGG ATACACATTA GTAAAGCACA GCCAGTCTGA ACAGATTCCT
    CCATGCCCGC TTGGAATGGC CAGATTGTGG GATGGCTATA GCTTGCTGTT CGTGGAAGGA
    CAAGAAAAGG CTCACAATCA AGACTTGGGT CTTGCTGGTT CCTGTTTGCC CCGATTCAGC
    ACCATGCCTT TTATTTACTG CAACATTAAT GAGGTCTGCT ACTATGCTAG CAGGAATGAC
    AAATCTTACT GGCTCTCTAC CACGGCCCCT ATCCCCATGA TGCCTGTGGG CAGCACTGAG
    ATTAAAAACT ACATTAGTCG CTGCTCAGTG TGTGAAGCTT CTTCCCAGGC TGTTGCTGTT
    CATAGCCAGG ATAACACAAT CCCACAATGC CCTGATGGAT GGAGGAGCCT CTGGATTGGA
    TACTCTTTCC TCATGCACAC AGCTGCTGGT GCTGAAGGTG GCGGTCAGTC CTTAGTATCT
    CCAGGTTCTT GCCTTGAAGA TTTTCGGGCT ACCCCATTTA TTGAATGCAG CGGAGCAAGA
    GGAACCTGCC ATTACTTTGC CAACAAGTAC AGCTTCTGGT TGACCACTGT GGAAGAAACA
    CGGCAGTTTG CTAGACCGCC CACTTCTGAA ACCTTGAAAT CTGGGCAGTT GAGAACACGT
    GTGAGCCGCT GCCAGGTGTG CATGAAGAAC TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952072.1
    CDS237..5312
    Translation

    Target ORF information:

    RefSeq Version XM_018096583.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 6 (col4a6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGTGACAC GACTTTGCCT TCTGGTCGCT GCCGCCTGCT TAGCTGCAGA ACTGGTGCTG 
    GTGCAAGGGG GAGGAAAAAA GTTCTATCAG GGGCCATGTG CCGGACGAGA TTGTAGTGGG
    GGCTGCAAAT GTTTTCCAGA AAAAGGAGCT CGGGGTCGAC CAGGGCAACT TGGGCCTCAG
    GGTCCAATGG GTCCACCAGG ATTTTCAGGA CCTGAAGGAG TAGCAGGGCA AAAAGGTGAA
    AGAGGAGATT ATGGCTTTCA AGGAGAAGCT GGACCTAAAG GAGACCTGGG TCATAGGGGA
    GTTACTGGCT TTGATGGAAA TGATGGAACA CCGGGTCATG TTGGCCAGGC AGGCCATAGA
    GGGTTACCTG GACTTGATGG CTGCAATGGC ACCATAGGCG ACTCAGGCTT TCCTGGTAGT
    AATGGCTTCC CTGGGCAATC TGGATTACCA GGTCCTATTG GTCCTAAAGG TCAGAAAGGG
    GAACCTTCTT TTATTTCAAG TGATATAAGA GGGCTGAAGG GAGAAGATGG AATACCTGGA
    CAAGAAGGTT TGCCTGGACC ACGTGGGCCT CCTGGACCAG AGGGTATGTC TGGCACAGCG
    GGATTTCCTG GTGTGCCGGG TCCTCCGGGT CTACCTGGAA TGCCAGGTCC AACAGGAAAC
    AGGGGTGTTG GTTATTATGG AGAGAATGGA GATAAGGGAG ACGTTGGGCC TCCAGGACCC
    CCGGGGCCGA GAGCATCTAC TGGGCCAGTG GAGTTTACTG AGTCAGCAAA AGGAATTCAA
    GGAGACAAGG GTGATAGAGG TCTTCCAGGG TTCCGAGGGG AGCAGGGATT TCCTGGCCTT
    GAGGGGTTTG GATTCAGTGG AGAGAAGGGA GAAAAGGGAA TACTTGGCCT TCCTGGCCCA
    AGAGGTTTTC CAGGTCTGGA TGGATCCAAT GGATATCCAG GCAGGAAGGG ACAGCCGGGG
    CTACCTGGTT TTGATGGACT TCATGGGTTC ACAGGAATGA AGGGTGAACA AGGAAACATT
    GGCTTACCTG GGACACCAAT ATACATAGAT GAGGATGGTT TAGCCATTAC AGGCCCACCA
    GGAGATCCAG GTCCTTCTGG ATTTCTCGGA ATGCCAGGAG ATGACGGTAT CAGTGGCCCA
    CCTGGCCCTC CCGGTCAACC TGGTTTACCA TCGTTTGTTA GAGGCAATCC TGGGCCATCT
    GGTTTTCCAG GTGTTCAAGG TCTTAAAGGG GAACAAGGAA ACCCAGGAAA ACCAGGTTTT
    GGGCCACCTG GAAATACAGG AAGAGTTGGC CAACCCGGAC CACCTGGTCC CCCTGGAGAA
    GATGGACCTT CTGGAGAAAT TGCTGGACAT CAAACAGCTT TAGGAGAACC AGGTTTACCA
    GGAGTCCCAG GAAATAGAGG TCCCCCAGGA CAAATCGGAC CTAAGGGATA TAAAGGAGCT
    GACGGAGACT GTGCTTGTGA AGGAGGCGAT CTTAGCAAGG GTAGTCCTGG ATCACCAGGG
    CTACCTGGTC AAAGAGGAAG TCCTGGTTTT TCTGGCCTAA ATGGAATACC AGGTGATCCT
    GGAAGAAGAG GCTCTGATGG ACTACCTGGT CCACCAGGTT CTCCTGGTCT ACCCTGGTTT
    CCTGGACCAA AAGGAGAAAA AGGAGATCCT GTGTATTCAA CCGGTAAAGG TCCACGTGGT
    GACCCTGGTT CTGTAGGTCC CAGGGGAGCA TTTGGTGCTG TGGGAAAACC TGGAAGATAT
    GGTTACCCAG GATTACCTGG TCTTAAAGGA GCTCCTGGCA CTGGTGGTTC TGGTGAGAAG
    GGATACCCAG GTTTGCTTGG GCCAAAGGGG AGTCGTGGGG AAACAGGACC TCCAGGTATT
    GGTTACCCAG GTTCTCCTGG ACCTCGTGGG CTTCCTGGTG ACCCAGGACA AGCAGGATTA
    CCAGGACAAC CAGGTCTGGC AGGGCCACCA GGAACAACAT TACCCTGTGT TATTGCTGGA
    GAACCAGGCC CACCAGGAAT CCGTGGCACA CCAGGATTTC CAGGTCGTAA AGGTAATATG
    GGATTCCCAG GAGTTCCAGG ACAACAGGGC TTTGATGGTG CTAAAGGACA ACCAGGCAAT
    CCAGGTTTTG GTGGAATTCC AGGGCCTCCT GGATTTCCTG GACCCCGTGG AGAAGAAGGT
    GCTCCAGGGT TCAATGGATT GCCAGGTAGT GAAGGACTTC CTGGAAAGTT TGGGATCCCA
    GGGTCCCCTG GTTTAAAAGG ATTGCGAGGA GAAGTATTGG GAGCAACACC TGGAGCTCCT
    GGTGAGCCAG GCCTACCTGG AATCAATGGA GACAAAGGCT TACCAGGAGA TGCTGGTTTA
    TCAGGACAGA GAGGTTTTGA TGGAAGGCCT GGACTGATGG GTGCTAAAGG AGAACGGGGT
    AACTCTGGTT TCCCTGGCAG CCCAGGTCCC AAAGGTCAAC CTGGCTTTGG AGGGTCAATG
    GGGATTAAAG GAAAACAGGG TCCTCCTGGA GATATTGGAT ACCCAGGCTC ACCAGGCTGC
    CAAGGTTTTC CTGGAAATAA AGGTGTACCT GGAGACAGAG GCATTCCGGG CATAGCTGGG
    ATGAAGGGAC GCCCAGGAGC TCCAGGGGAT GCAGGACGTC CTGGTGAATT AGGTCTCCCT
    GGGCTACCTG GAACAGGTGG CCGTGAAGGT CTTCCAGGGT TCCAAGGACT TAAAGGAGAG
    AAAGGCTTTC CTGGTGTGAG GGGATTTGCT GGAATGCCTG GTATTCCAGG ACTTCCTGGA
    GTCAGTGGAT ATAAAGGAAT TTGTGGCTCA CCTGGACAAG ATGGACAAAA AGGAAATGAT
    GGCATTAAGG GAGTAAAAGG TTTACCTGGC GATCGTGGTC CACCAGGTGC TGTCATGCCA
    CCACCACCCG GCCTTCTTTC TAAAGGTGCT CAAGGAGAAC AAGGACCAAG TGGCCCTCCT
    GGATTTACAG GACCAAGAGG TGATAAAGGC TTTCCTGGAC TTCCAGGAAT ACCTGGGAAC
    CCTGGTTTTC CAGGCAACCC AGGCAGAGAT AAAGGTTTTC CAGGTGACCC AGGATTTCCC
    GGAGCATTTG GAAGACCAGG ATTTCCTGGT CCAAAAGGAG AACCTGGAAT AATTGGATTT
    CCTGGAATGC CTGGACCAAA TGGTGCTGAC GGTATCCCGG GAGTTTCTGG CTTTCCTGGC
    AGTCCTGGCC TACCAGGACC CAAGGGAGAA AGAGGAGAGA CTTTAGGACA ACCTGGACGT
    CCAGGAGTTA AAGGAGAGAC TGGCATACCA GGAATTTCAG GTCTAAGAGG TTTTGATGGC
    ATGCCTGGAG ATGTTGGGTC AATAGGAATA TCTGGAAATC CTGGACCAAA GGGTCCTCCA
    GGAGAGTTTG GACGGATAGG ACAACCTGGA AATCCGGGGC AACAGGGATA TAGAGGAAAC
    CCTGGATTAG TGGGTAGTTC TGGATTACCA GGTTCTCCAG GATCCCCAGG ATACCCAGGG
    GCACCAGGTC TTAAAGGTTT TCCTGGATCT CAAGGTATTG GTGGCTTAAA TGGCTTGGCT
    GGAAGTAAAG GGTCCCCTGG AACACCAGGG CTTAGTGAGG CTGGACGACC AGGAACCCCT
    GGATTTCCAG GGCCAAAAGG TGAAAGAGGT TCTCCCAGCA CAATATCGGG GCAACCAGGA
    TTTCCTGGAT CTAAAGGATT TCCTGGAGAA AGAGGTCTTC CTGGTGCTGT TGGCTTACCT
    GGTCTTCCTG GACCACCAGG CTTCTCATTC CCCTCTGCCA TCCCAGGGAG ACCTGGTGAC
    CCTGGCTTTC CTGGACGAGA TGGGGAAGCA GGACTTCCTG GCCTGCCAGG GCCACGTGGC
    CCACCTGGCC CAGGATCTAA TCAAGGGGAT ACAGGGGACT CAGGATACCC AGGCATTCCT
    GGCAGGCCTG GCATAAAGGG TGAACCTGGA ATTCCAGGAA ACCCAGGAAG CCCTGGAGCT
    CCTGGACTGA AAGGTCTAAG AGGTGATCCA GGTTTCATTG GTAGCCCAGG CAAGGAAGGT
    TCTGTAGGAG ATCCTGGCTT TCCAGGACTA AAGGGAGAAA GGGGCAACCC AGGACTTCCT
    GGTCCCCAAG GACGACCTGG CCCTCCCCCA TCGATAGAGG ATTACCAGAT TCCTATTGGA
    TTACCAGGTC TGCCTGGAAT TAATGGTTTT GATGGTGTTC TTGGGGAAAT CGGATCACCA
    GGACCAAATG GACCACCAGG AGCAAAAGGA TTTCCAGGCA GATCTGGGCA AATGGGAAGA
    AATGGGCCAC CTGGTGCTCC TGGTCCTATT GGTGATCCTG GCGAAATTGG TATTCCTGGT
    ACTTCTGGAC TTGAAGGTTC TCCTGGTAAG CAGGGTGCAG TTGGCTTACC CGGAATGCCC
    GGACGGAGTG TGGGTGTTGG ATACACATTA GTAAAGCACA GCCAGTCTGA ACAGATTCCT
    CCATGCCCGC TTGGAATGGC CAGATTGTGG GATGGCTATA GCTTGCTGTT CGTGGAAGGA
    CAAGAAAAGG CTCACAATCA AGACTTGGGT CTTGCTGGTT CCTGTTTGCC CCGATTCAGC
    ACCATGCCTT TTATTTACTG CAACATTAAT GAGGTCTGCT ACTATGCTAG CAGGAATGAC
    AAATCTTACT GGCTCTCTAC CACGGCCCCT ATCCCCATGA TGCCTGTGGG CAGCACTGAG
    ATTAAAAACT ACATTAGTCG CTGCTCAGTG TGTGAAGCTT CTTCCCAGGC TGTTGCTGTT
    CATAGCCAGG ATAACACAAT CCCACAATGC CCTGATGGAT GGAGGAGCCT CTGGATTGGA
    TACTCTTTCC TCATGCACAC AGCTGCTGGT GCTGAAGGTG GCGGTCAGTC CTTAGTATCT
    CCAGGTTCTT GCCTTGAAGA TTTTCGGGCT ACCCCATTTA TTGAATGCAG CGGAGCAAGA
    GGAACCTGCC ATTACTTTGC CAACAAGTAC AGCTTCTGGT TGACCACTGT GGAAGAAACA
    CGGCAGTTTG CTAGACCGCC CACTTCTGAA ACCTTGAAAT CTGGGCAGTT GAGAACACGT
    GTGAGCCGCT GCCAGGTGTG CATGAAGAAC TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.