gramd1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gramd1a
Entrez Gene ID	100492510
Full Name	GRAM domain containing 1A
General protein information	Preferred Names GRAM domain containing 1A Names GRAM domain-containing protein 1A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967321.2	XP_012822775.1	GRAM domain-containing protein 1A
XM_031905433.1	XP_031761293.1	protein Aster-A isoform X3
XM_031905435.1	XP_031761295.1	protein Aster-A isoform X5
XM_031905432.1	XP_031761292.1	protein Aster-A isoform X2
XM_031905431.1	XP_031761291.1	protein Aster-A isoform X1
XM_031905434.1	XP_031761294.1	protein Aster-A isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GRAMD1A	XP_005616784.1
Bos taurus (cattle)	GRAMD1A	XP_002694974.1
Rattus norvegicus (Norway rat)	Gramd1a	NP_001014182.1
Pan troglodytes (chimpanzee)	GRAMD1A	XP_001157243.2
Mus musculus (house mouse)	Gramd1a	NP_082174.3
Xenopus tropicalis (tropical clawed frog)	gramd1a	XP_002940577.2
Danio rerio (zebrafish)	gramd1a	XP_683776.6
Homo sapiens (human)	GRAMD1A	NP_065946.2

Gene Ontology

The following gramd1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gramd1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35397	XM_012967321.2 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa61773	XM_031905431.1 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa61774	XM_031905432.1 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa61775	XM_031905433.1 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa61776	XM_031905434.1 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa61777	XM_031905435.1 Latest version!	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gramd1a ( XM_012967321.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822775.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), mRNA.

Price & Availability↑

CloneID	OXa35397
Clone ID Related Accession (Same CDS sequence)	XM_012967321.2
Accession Version	XM_012967321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GRAM domain-containing protein 1A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGTGCC TGGCCTGTCA GGGATGGAGC GCCACTTTCC TGAGCACTGG CAGCATCACT 
CCCCGCAGCA CTCCCAGTAA CTCCCCTGCT GTGCGGAGGC GTCTGGTGAG CAGCGAAGTT 
GACAATATGG TGGAAAGGGG ATCCGAAGGC TCCGCAGAGC AGTCCCCCAA CACTTTAACC 
CCCACGCTAC CCCAGTCTGC CGCTGTGCCA GGGACCCAGG GCAATGCCCA GCAACCCACG 
CAGTCCAACT CCCAGCCTGG AACCCCACTC ACGGCGCAGC TCAGCGGGCG AGGATTCATA 
CGGAACAGCA AGAAGATGCA GAGCTGGTAC AGTATGCTGA GCCCCACGTA CAAGCAGCGC 
AACGAAAACT TTCGCAAGAT TTTCAAGAAG CTTCCGGATT CCGAGCGCCT CATTGTTGAC 
TACTCCTGTG CGCTGCAGAA GGATATCTTA CTCCAGGGAA GGCTCTATTT GTCGGAGAAC 
TGGATCTGTT TTTATAGCAA CATTTTCCGC TGGGAAACCA CGGTGAGTCC GACAGTTTGC 
CCATTGCGAT TGCCTGTCGC TGTAACTTTT TCCATTTCAC CAAACGTCTC ACCTTGCCCT 
TTTTTTATTT TCCAGCACTT TTTTACCTCC TTCGGCGCCC GAGATCGGTC GTTTCTGCTG 
ATTTTCCGTC TCTGGCAGAA TGCTCTTCTA GATAAGACCC TGTCCCCGCG GGAGCTGTGG 
CACATTGTAC ACCAGTGTTA CGGGACTGAG CTGGGACTGA CCAGTGAGGA TGAAGATTAT 
GTGTCGCCTC GAGACCTGCT GAACGGCTTG GGGGGGAGTG AGGATTTCCC TGAGAGCACC 
GACCTGAGTG ACACTTCATC CCGCGGTGAC ACCAAACTCA GTGCCAGCCC CCAACTGCCT 
GCGACCGACT CCTTCACCTC CCTCGCCAGC GCCGACAGCA TGATCCCTCC AGCGGAGGAT 
GCGGCCGCCC AATCAGATGC CCAGTCAGAC TCCCAGTTGG AAGGCTCGTC CAGTCAGACG 
GTGACTCCTC TGTCGGAGAT GGCTGGCTCC GCCCTCCTGG ACTCCCTTCC TGCCCTGGAT 
TTATTGCCCA GTGAAGAACT GCCCACTGAC CCCAGCAACA ACTCCACCCC CAGCTCCACG 
CAGGACGAGG AAGCCAGTAC AGTGCCTAAA GACACCCCCT CGTCGGACCT GACCGGCAGG 
CTACATATCA ATGCCGTGTA TCACATCAGC GCAGAGCACC TACAGCACGC ACTGACCACC 
GACACCCAAT TCATGAACGA TTTCATGGAG CAGCGGAAAT TCACAGATAT CACAGTGAAT 
CCCTGGATGC GGGACGGCAA CGGGAAACAG TCTCGGATAC TGAATTACAC AATCCCCATC 
AATAACCCTC TGGGGCCCAA ATCTGCCCCG GCTATAGAAA CCCAGATTCT GCACAGTGTG 
AAGGGATCAG TCTGTGTCCT CGACACCCAG GTTATAACTC AGGGCATCCC CTACCAGGAT 
TATTTCTACA CCTCTCACCG GTACTGCATC AGCAGCGTGG GCAAGAACAA AGCACGCCTC 
AGGGTTTCCT CTGAGATCTG CTACAGGAAG CAGCCATGGA GCCTGGTACG AGCCATCATA 
GAGAAGAACT CCTGGAATGG CATGGAGGAG CACTTCAGCC TGCTGGCCGA TGCGGTGGCA 
AAGCTGGACC AGTCCTGCGC GGAGGAGATG GGGGGCAGAG AAAAGCCTGC GCTACGGCGG 
AGGAAGCGCA CTTTAAGCTG GAAGTCCCAT CACAACGATA CCAATAACAC CGCCTCCCCC 
CAGATTGAAA GCGAAAGAGT TTCCCGTAAC TCCAGAGTTT CAGGCAGCAT CTCCTCTCAG 
CTCTCCCAGC TCTCCAACCG AGACACGTCC AGCAACATCC CCAACGCGCT GTTTGTTATC 
AGTATTGTGC TGCTGGTCCT GGTGGTCCTG AACATGATGC TCTTCTATCG CCTCTGGTCG 
CTGGAGCACA CGGCTCGGAC ATTCCAGACG TGGCAAAACT ACGCCGTGTC CCAAGGGAGG 
TTCCCCAAGA GTTCCGCCGA CTGGGCCGAG ATCCTCGAGC TACAGAAGCG CTTCCACAAC 
GCCGAGGTGC AGAAATGGAA GCAGACGCTC CGAGCCTCTG TGCAGCTGCT GGATGAGATG 
AGGATGTCTC TGCAGAGACT GCAGCAGGGA GTCCTGATGG CCGATTCCGA CACAGAACCT 
CACGAGGAAG ACGCCCTGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822775.1
CDS	246..2489
Translation	MECLACQGWSATFLSTGSITPRSTPSNSPAVRRRLVSSEVDNMVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKKMQSWYSMLSPTYKQRNENFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTVSPTVCPLRLPVAVTFSISPNVSPCPFFIFQHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTVPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPKSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_012967321.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967321.2

ORF Insert Sequence:

ATGGAGTGCC TGGCCTGTCA GGGATGGAGC GCCACTTTCC TGAGCACTGG CAGCATCACT 
CCCCGCAGCA CTCCCAGTAA CTCCCCTGCT GTGCGGAGGC GTCTGGTGAG CAGCGAAGTT 
GACAATATGG TGGAAAGGGG ATCCGAAGGC TCCGCAGAGC AGTCCCCCAA CACTTTAACC 
CCCACGCTAC CCCAGTCTGC CGCTGTGCCA GGGACCCAGG GCAATGCCCA GCAACCCACG 
CAGTCCAACT CCCAGCCTGG AACCCCACTC ACGGCGCAGC TCAGCGGGCG AGGATTCATA 
CGGAACAGCA AGAAGATGCA GAGCTGGTAC AGTATGCTGA GCCCCACGTA CAAGCAGCGC 
AACGAAAACT TTCGCAAGAT TTTCAAGAAG CTTCCGGATT CCGAGCGCCT CATTGTTGAC 
TACTCCTGTG CGCTGCAGAA GGATATCTTA CTCCAGGGAA GGCTCTATTT GTCGGAGAAC 
TGGATCTGTT TTTATAGCAA CATTTTCCGC TGGGAAACCA CGGTGAGTCC GACAGTTTGC 
CCATTGCGAT TGCCTGTCGC TGTAACTTTT TCCATTTCAC CAAACGTCTC ACCTTGCCCT 
TTTTTTATTT TCCAGCACTT TTTTACCTCC TTCGGCGCCC GAGATCGGTC GTTTCTGCTG 
ATTTTCCGTC TCTGGCAGAA TGCTCTTCTA GATAAGACCC TGTCCCCGCG GGAGCTGTGG 
CACATTGTAC ACCAGTGTTA CGGGACTGAG CTGGGACTGA CCAGTGAGGA TGAAGATTAT 
GTGTCGCCTC GAGACCTGCT GAACGGCTTG GGGGGGAGTG AGGATTTCCC TGAGAGCACC 
GACCTGAGTG ACACTTCATC CCGCGGTGAC ACCAAACTCA GTGCCAGCCC CCAACTGCCT 
GCGACCGACT CCTTCACCTC CCTCGCCAGC GCCGACAGCA TGATCCCTCC AGCGGAGGAT 
GCGGCCGCCC AATCAGATGC CCAGTCAGAC TCCCAGTTGG AAGGCTCGTC CAGTCAGACG 
GTGACTCCTC TGTCGGAGAT GGCTGGCTCC GCCCTCCTGG ACTCCCTTCC TGCCCTGGAT 
TTATTGCCCA GTGAAGAACT GCCCACTGAC CCCAGCAACA ACTCCACCCC CAGCTCCACG 
CAGGACGAGG AAGCCAGTAC AGTGCCTAAA GACACCCCCT CGTCGGACCT GACCGGCAGG 
CTACATATCA ATGCCGTGTA TCACATCAGC GCAGAGCACC TACAGCACGC ACTGACCACC 
GACACCCAAT TCATGAACGA TTTCATGGAG CAGCGGAAAT TCACAGATAT CACAGTGAAT 
CCCTGGATGC GGGACGGCAA CGGGAAACAG TCTCGGATAC TGAATTACAC AATCCCCATC 
AATAACCCTC TGGGGCCCAA ATCTGCCCCG GCTATAGAAA CCCAGATTCT GCACAGTGTG 
AAGGGATCAG TCTGTGTCCT CGACACCCAG GTTATAACTC AGGGCATCCC CTACCAGGAT 
TATTTCTACA CCTCTCACCG GTACTGCATC AGCAGCGTGG GCAAGAACAA AGCACGCCTC 
AGGGTTTCCT CTGAGATCTG CTACAGGAAG CAGCCATGGA GCCTGGTACG AGCCATCATA 
GAGAAGAACT CCTGGAATGG CATGGAGGAG CACTTCAGCC TGCTGGCCGA TGCGGTGGCA 
AAGCTGGACC AGTCCTGCGC GGAGGAGATG GGGGGCAGAG AAAAGCCTGC GCTACGGCGG 
AGGAAGCGCA CTTTAAGCTG GAAGTCCCAT CACAACGATA CCAATAACAC CGCCTCCCCC 
CAGATTGAAA GCGAAAGAGT TTCCCGTAAC TCCAGAGTTT CAGGCAGCAT CTCCTCTCAG 
CTCTCCCAGC TCTCCAACCG AGACACGTCC AGCAACATCC CCAACGCGCT GTTTGTTATC 
AGTATTGTGC TGCTGGTCCT GGTGGTCCTG AACATGATGC TCTTCTATCG CCTCTGGTCG 
CTGGAGCACA CGGCTCGGAC ATTCCAGACG TGGCAAAACT ACGCCGTGTC CCAAGGGAGG 
TTCCCCAAGA GTTCCGCCGA CTGGGCCGAG ATCCTCGAGC TACAGAAGCG CTTCCACAAC 
GCCGAGGTGC AGAAATGGAA GCAGACGCTC CGAGCCTCTG TGCAGCTGCT GGATGAGATG 
AGGATGTCTC TGCAGAGACT GCAGCAGGGA GTCCTGATGG CCGATTCCGA CACAGAACCT 
CACGAGGAAG ACGCCCTGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1a ( XM_031905431.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761291.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61773
Clone ID Related Accession (Same CDS sequence)	XM_031905431.1
Accession Version	XM_031905431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCA CCAGACCCAA TTCCTTTGCC GTAGCCATGG AAACCCTCAA GCAGTTGCCG 
TCCCTAAAGC CCCCGGAATC GTGGTCCTGT TCATCCACCC CGAGTGCCAG GCGGAGGCGC 
CTCAAGTCAA AGAAGGGTCA GAGGACCCCC GAGTCTGGAG GAAGAAGCGA CGCAGAGGGG 
TCGGCCGTAA GAGGGAGGAG CCTGAAACCC CCAGCTATAG AAGTGACCCC GTCCAGTGAG 
GATGAGGCCT GGTCCAACTG CTCCACACCC AGTGCTTCGC CACAGAGGCG CCGCGGCCTG 
TTTAGGAAGT GGCTAAAGAG GCGAGAGGCA AAAAGCTGCA CTGGCAGCAT CACTCCCCGC 
AGCACTCCCA GTAACTCCCC TGCTGTGCGG AGGCGTCTGG TGAGCAGCGA AGTTGACAAT 
ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGAAGA TGCAGAGCTG GTACAGTATG CTGAGCCCCA CGTACAAGCA GCGCAACGAA 
GACTTTCGCA AGATTTTCAA GAAGCTTCCG GATTCCGAGC GCCTCATTGT TGACTACTCC 
TGTGCGCTGC AGAAGGATAT CTTACTCCAG GGAAGGCTCT ATTTGTCGGA GAACTGGATC 
TGTTTTTATA GCAACATTTT CCGCTGGGAA ACCACGATCA TGATCCAGCT GAAGGACATA 
CAATGCATCA AGAAGGAGAA GACAGCCAAG CTGATTCCCA ACGCCATCCA AGTATGCACT 
GAAACCGAGA AGCACTTTTT TACCTCCTTC GGCGCCCGAG ATCGGTCGTT TCTGCTGATT 
TTCCGTCTCT GGCAGAATGC TCTTCTAGAT AAGACCCTGT CCCCGCGGGA GCTGTGGCAC 
ATTGTACACC AGTGTTACGG GACTGAGCTG GGACTGACCA GTGAGGATGA AGATTATGTG 
TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG GGGAGTGAGG ATTTCCCTGA GAGCACCGAC 
CTGAGTGACA CTTCATCCCG CGGTGACACC AAACTCAGTG CCAGCCCCCA ACTGCCTGCG 
ACCGACTCCT TCACCTCCCT CGCCAGCGCC GACAGCATGA TCCCTCCAGC GGAGGATGCG 
GCCGCCCAAT CAGATGCCCA GTCAGACTCC CAGTTGGAAG GCTCGTCCAG TCAGACGGTG 
ACTCCTCTGT CGGAGATGGC TGGCTCCGCC CTCCTGGACT CCCTTCCTGC CCTGGATTTA 
TTGCCCAGTG AAGAACTGCC CACTGACCCC AGCAACAACT CCACCCCCAG CTCCACGCAG 
GACGAGGAAG CCAGTACAGC GCCTAAAGAC ACCCCCTCGT CGGACCTGAC CGGCAGGCTA 
CATATCAATG CCGTGTATCA CATCAGCGCA GAGCACCTAC AGCACGCACT GACCACCGAC 
ACCCAATTCA TGAACGATTT CATGGAGCAG CGGAAATTCA CAGATATCAC AGTGAATCCC 
TGGATGCGGG ACGGCAACGG GAAACAGTCT CGGATACTGA ATTACACAAT CCCCATCAAT 
AACCCTCTGG GGCCCAAATC TGCCCCGGCT ATAGAAACCC AGATTCTGCA CAGTGTGAAG 
GGGTCAGTCT GTGTTCTCGA CACCCAGGTT ATAACTCAGG GCATCCCCTA CCAGGATTAT 
TTCTACACCT CTCACCGGTA CTGCATCAGC AGCGTGGGCA AGAACAAGGC ACGCCTCAGG 
GTTTCCTCTG AGATCTGCTA CAGGAAGCAG CCATGGAGCC TGGTACGAGC CATCATAGAG 
AAGAACTCCT GGAATGGCAT GGAGGAGCAC TTCAGCCTGC TGGCCGATGC GGTGGCAAAG 
CTGGACCAGT CCTGCGCGGA GGAGATGGGG GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG 
AAGCGCACTT TAAGCTGGAA GTCCCATCAC AACGATACCA ATAACACCGC CTCCCCCCAG 
ATTGAAAGCG AAAGAGTTTC CCGTAACTCC AGAGTTTCAG GCAGCATCTC CTCTCAGCTC 
TCCCAGCTCT CCAACCGAGA CACGTCCAGC AACATCCCCA ACGCGCTGTT TGTTATCAGT 
ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC ATGATGCTCT TCTACCGCCT CTGGTCGCTG 
GAGCACACGG CTCGGACATT CCAGACGTGG CAAAACTACG CCGTGTCCCA AGGGAGGTTC 
CCCCAGAGTT CCGCCGACTG GGCCGAGATC CTCGAGCTAC AGAAGCGCTT CCACAACGCC 
GAGGTGCAGA AATGGAAGCA GACGCTCCGA GCCTCTGTGC AGCTGCTGGA TGAGATGAGG 
ATGTCTCTGC AGAGACTGCA GCAGGGAGTC CTGATGGCCG ATTCCGACAC AGAACCTCAC 
GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761291.1
CDS	1276..3816
Translation	MRGTRPNSFAVAMETLKQLPSLKPPESWSCSSTPSARRRRLKSKKGQRTPESGGRSDAEGSAVRGRSLKPPAIEVTPSSEDEAWSNCSTPSASPQRRRGLFRKWLKRREAKSCTGSITPRSTPSNSPAVRRRLVSSEVDNMVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKKMQSWYSMLSPTYKQRNEDFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTIMIQLKDIQCIKKEKTAKLIPNAIQVCTETEKHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTAPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPQSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_031905431.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905431.1

ORF Insert Sequence:

ATGAGAGGCA CCAGACCCAA TTCCTTTGCC GTAGCCATGG AAACCCTCAA GCAGTTGCCG 
TCCCTAAAGC CCCCGGAATC GTGGTCCTGT TCATCCACCC CGAGTGCCAG GCGGAGGCGC 
CTCAAGTCAA AGAAGGGTCA GAGGACCCCC GAGTCTGGAG GAAGAAGCGA CGCAGAGGGG 
TCGGCCGTAA GAGGGAGGAG CCTGAAACCC CCAGCTATAG AAGTGACCCC GTCCAGTGAG 
GATGAGGCCT GGTCCAACTG CTCCACACCC AGTGCTTCGC CACAGAGGCG CCGCGGCCTG 
TTTAGGAAGT GGCTAAAGAG GCGAGAGGCA AAAAGCTGCA CTGGCAGCAT CACTCCCCGC 
AGCACTCCCA GTAACTCCCC TGCTGTGCGG AGGCGTCTGG TGAGCAGCGA AGTTGACAAT 
ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGAAGA TGCAGAGCTG GTACAGTATG CTGAGCCCCA CGTACAAGCA GCGCAACGAA 
GACTTTCGCA AGATTTTCAA GAAGCTTCCG GATTCCGAGC GCCTCATTGT TGACTACTCC 
TGTGCGCTGC AGAAGGATAT CTTACTCCAG GGAAGGCTCT ATTTGTCGGA GAACTGGATC 
TGTTTTTATA GCAACATTTT CCGCTGGGAA ACCACGATCA TGATCCAGCT GAAGGACATA 
CAATGCATCA AGAAGGAGAA GACAGCCAAG CTGATTCCCA ACGCCATCCA AGTATGCACT 
GAAACCGAGA AGCACTTTTT TACCTCCTTC GGCGCCCGAG ATCGGTCGTT TCTGCTGATT 
TTCCGTCTCT GGCAGAATGC TCTTCTAGAT AAGACCCTGT CCCCGCGGGA GCTGTGGCAC 
ATTGTACACC AGTGTTACGG GACTGAGCTG GGACTGACCA GTGAGGATGA AGATTATGTG 
TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG GGGAGTGAGG ATTTCCCTGA GAGCACCGAC 
CTGAGTGACA CTTCATCCCG CGGTGACACC AAACTCAGTG CCAGCCCCCA ACTGCCTGCG 
ACCGACTCCT TCACCTCCCT CGCCAGCGCC GACAGCATGA TCCCTCCAGC GGAGGATGCG 
GCCGCCCAAT CAGATGCCCA GTCAGACTCC CAGTTGGAAG GCTCGTCCAG TCAGACGGTG 
ACTCCTCTGT CGGAGATGGC TGGCTCCGCC CTCCTGGACT CCCTTCCTGC CCTGGATTTA 
TTGCCCAGTG AAGAACTGCC CACTGACCCC AGCAACAACT CCACCCCCAG CTCCACGCAG 
GACGAGGAAG CCAGTACAGC GCCTAAAGAC ACCCCCTCGT CGGACCTGAC CGGCAGGCTA 
CATATCAATG CCGTGTATCA CATCAGCGCA GAGCACCTAC AGCACGCACT GACCACCGAC 
ACCCAATTCA TGAACGATTT CATGGAGCAG CGGAAATTCA CAGATATCAC AGTGAATCCC 
TGGATGCGGG ACGGCAACGG GAAACAGTCT CGGATACTGA ATTACACAAT CCCCATCAAT 
AACCCTCTGG GGCCCAAATC TGCCCCGGCT ATAGAAACCC AGATTCTGCA CAGTGTGAAG 
GGGTCAGTCT GTGTTCTCGA CACCCAGGTT ATAACTCAGG GCATCCCCTA CCAGGATTAT 
TTCTACACCT CTCACCGGTA CTGCATCAGC AGCGTGGGCA AGAACAAGGC ACGCCTCAGG 
GTTTCCTCTG AGATCTGCTA CAGGAAGCAG CCATGGAGCC TGGTACGAGC CATCATAGAG 
AAGAACTCCT GGAATGGCAT GGAGGAGCAC TTCAGCCTGC TGGCCGATGC GGTGGCAAAG 
CTGGACCAGT CCTGCGCGGA GGAGATGGGG GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG 
AAGCGCACTT TAAGCTGGAA GTCCCATCAC AACGATACCA ATAACACCGC CTCCCCCCAG 
ATTGAAAGCG AAAGAGTTTC CCGTAACTCC AGAGTTTCAG GCAGCATCTC CTCTCAGCTC 
TCCCAGCTCT CCAACCGAGA CACGTCCAGC AACATCCCCA ACGCGCTGTT TGTTATCAGT 
ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC ATGATGCTCT TCTACCGCCT CTGGTCGCTG 
GAGCACACGG CTCGGACATT CCAGACGTGG CAAAACTACG CCGTGTCCCA AGGGAGGTTC 
CCCCAGAGTT CCGCCGACTG GGCCGAGATC CTCGAGCTAC AGAAGCGCTT CCACAACGCC 
GAGGTGCAGA AATGGAAGCA GACGCTCCGA GCCTCTGTGC AGCTGCTGGA TGAGATGAGG 
ATGTCTCTGC AGAGACTGCA GCAGGGAGTC CTGATGGCCG ATTCCGACAC AGAACCTCAC 
GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1a ( XM_031905432.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761292.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa61774
Clone ID Related Accession (Same CDS sequence)	XM_031905432.1
Accession Version	XM_031905432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCA CCAGACCCAA TTCCTTTGCC GTAGCCATGG AAACCCTCAA GCAGTTGCCG 
TCCCTAAAGC CCCCGGAATC GTGGTCCTGT TCATCCACCC CGAGTGCCAG GCGGAGGCGC 
CTCAAGTCAA AGAAGGGTCA GAGGACCCCC GAGTCTGGAG GAAGAAGCGA CGCAGAGGGG 
TCGGCCGTAA GAGGGAGGAG CCTGAAACCC CCAGCTATAG AAGTGACCCC GTCCAGTGAG 
GATGAGGCCT GGTCCAACTG CTCCACACCC AGTGCTTCGC CACAGAGGCG CCGCGGCCTG 
TTTAGGAAGT GGCTAAAGAG GCGAGAGGCA AAAAGCTGCA CTGGCAGCAT CACTCCCCGC 
AGCACTCCCA GTAACTCCCC TGCTGTGCGG AGGCGTCTGG TGAGCAGCGA AGTTGACAAT 
ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGATGC TGAGCCCCAC GTACAAGCAG CGCAACGAAG ACTTTCGCAA GATTTTCAAG 
AAGCTTCCGG ATTCCGAGCG CCTCATTGTT GACTACTCCT GTGCGCTGCA GAAGGATATC 
TTACTCCAGG GAAGGCTCTA TTTGTCGGAG AACTGGATCT GTTTTTATAG CAACATTTTC 
CGCTGGGAAA CCACGATCAT GATCCAGCTG AAGGACATAC AATGCATCAA GAAGGAGAAG 
ACAGCCAAGC TGATTCCCAA CGCCATCCAA GTATGCACTG AAACCGAGAA GCACTTTTTT 
ACCTCCTTCG GCGCCCGAGA TCGGTCGTTT CTGCTGATTT TCCGTCTCTG GCAGAATGCT 
CTTCTAGATA AGACCCTGTC CCCGCGGGAG CTGTGGCACA TTGTACACCA GTGTTACGGG 
ACTGAGCTGG GACTGACCAG TGAGGATGAA GATTATGTGT CGCCTCGAGA CCTGCTGAAC 
GGCTTGGGGG GGAGTGAGGA TTTCCCTGAG AGCACCGACC TGAGTGACAC TTCATCCCGC 
GGTGACACCA AACTCAGTGC CAGCCCCCAA CTGCCTGCGA CCGACTCCTT CACCTCCCTC 
GCCAGCGCCG ACAGCATGAT CCCTCCAGCG GAGGATGCGG CCGCCCAATC AGATGCCCAG 
TCAGACTCCC AGTTGGAAGG CTCGTCCAGT CAGACGGTGA CTCCTCTGTC GGAGATGGCT 
GGCTCCGCCC TCCTGGACTC CCTTCCTGCC CTGGATTTAT TGCCCAGTGA AGAACTGCCC 
ACTGACCCCA GCAACAACTC CACCCCCAGC TCCACGCAGG ACGAGGAAGC CAGTACAGCG 
CCTAAAGACA CCCCCTCGTC GGACCTGACC GGCAGGCTAC ATATCAATGC CGTGTATCAC 
ATCAGCGCAG AGCACCTACA GCACGCACTG ACCACCGACA CCCAATTCAT GAACGATTTC 
ATGGAGCAGC GGAAATTCAC AGATATCACA GTGAATCCCT GGATGCGGGA CGGCAACGGG 
AAACAGTCTC GGATACTGAA TTACACAATC CCCATCAATA ACCCTCTGGG GCCCAAATCT 
GCCCCGGCTA TAGAAACCCA GATTCTGCAC AGTGTGAAGG GGTCAGTCTG TGTTCTCGAC 
ACCCAGGTTA TAACTCAGGG CATCCCCTAC CAGGATTATT TCTACACCTC TCACCGGTAC 
TGCATCAGCA GCGTGGGCAA GAACAAGGCA CGCCTCAGGG TTTCCTCTGA GATCTGCTAC 
AGGAAGCAGC CATGGAGCCT GGTACGAGCC ATCATAGAGA AGAACTCCTG GAATGGCATG 
GAGGAGCACT TCAGCCTGCT GGCCGATGCG GTGGCAAAGC TGGACCAGTC CTGCGCGGAG 
GAGATGGGGG GCAGAGAAAA GCCTGCGCTA CGGCGGAGGA AGCGCACTTT AAGCTGGAAG 
TCCCATCACA ACGATACCAA TAACACCGCC TCCCCCCAGA TTGAAAGCGA AAGAGTTTCC 
CGTAACTCCA GAGTTTCAGG CAGCATCTCC TCTCAGCTCT CCCAGCTCTC CAACCGAGAC 
ACGTCCAGCA ACATCCCCAA CGCGCTGTTT GTTATCAGTA TTGTGCTGCT GGTCCTGGTG 
GTCCTGAACA TGATGCTCTT CTACCGCCTC TGGTCGCTGG AGCACACGGC TCGGACATTC 
CAGACGTGGC AAAACTACGC CGTGTCCCAA GGGAGGTTCC CCCAGAGTTC CGCCGACTGG 
GCCGAGATCC TCGAGCTACA GAAGCGCTTC CACAACGCCG AGGTGCAGAA ATGGAAGCAG 
ACGCTCCGAG CCTCTGTGCA GCTGCTGGAT GAGATGAGGA TGTCTCTGCA GAGACTGCAG 
CAGGGAGTCC TGATGGCCGA TTCCGACACA GAACCTCACG AGGAAGACGC CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761292.1
CDS	1282..3801
Translation	MRGTRPNSFAVAMETLKQLPSLKPPESWSCSSTPSARRRRLKSKKGQRTPESGGRSDAEGSAVRGRSLKPPAIEVTPSSEDEAWSNCSTPSASPQRRRGLFRKWLKRREAKSCTGSITPRSTPSNSPAVRRRLVSSEVDNMVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKMLSPTYKQRNEDFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTIMIQLKDIQCIKKEKTAKLIPNAIQVCTETEKHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTAPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPQSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_031905432.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905432.1

ORF Insert Sequence:

ATGAGAGGCA CCAGACCCAA TTCCTTTGCC GTAGCCATGG AAACCCTCAA GCAGTTGCCG 
TCCCTAAAGC CCCCGGAATC GTGGTCCTGT TCATCCACCC CGAGTGCCAG GCGGAGGCGC 
CTCAAGTCAA AGAAGGGTCA GAGGACCCCC GAGTCTGGAG GAAGAAGCGA CGCAGAGGGG 
TCGGCCGTAA GAGGGAGGAG CCTGAAACCC CCAGCTATAG AAGTGACCCC GTCCAGTGAG 
GATGAGGCCT GGTCCAACTG CTCCACACCC AGTGCTTCGC CACAGAGGCG CCGCGGCCTG 
TTTAGGAAGT GGCTAAAGAG GCGAGAGGCA AAAAGCTGCA CTGGCAGCAT CACTCCCCGC 
AGCACTCCCA GTAACTCCCC TGCTGTGCGG AGGCGTCTGG TGAGCAGCGA AGTTGACAAT 
ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGATGC TGAGCCCCAC GTACAAGCAG CGCAACGAAG ACTTTCGCAA GATTTTCAAG 
AAGCTTCCGG ATTCCGAGCG CCTCATTGTT GACTACTCCT GTGCGCTGCA GAAGGATATC 
TTACTCCAGG GAAGGCTCTA TTTGTCGGAG AACTGGATCT GTTTTTATAG CAACATTTTC 
CGCTGGGAAA CCACGATCAT GATCCAGCTG AAGGACATAC AATGCATCAA GAAGGAGAAG 
ACAGCCAAGC TGATTCCCAA CGCCATCCAA GTATGCACTG AAACCGAGAA GCACTTTTTT 
ACCTCCTTCG GCGCCCGAGA TCGGTCGTTT CTGCTGATTT TCCGTCTCTG GCAGAATGCT 
CTTCTAGATA AGACCCTGTC CCCGCGGGAG CTGTGGCACA TTGTACACCA GTGTTACGGG 
ACTGAGCTGG GACTGACCAG TGAGGATGAA GATTATGTGT CGCCTCGAGA CCTGCTGAAC 
GGCTTGGGGG GGAGTGAGGA TTTCCCTGAG AGCACCGACC TGAGTGACAC TTCATCCCGC 
GGTGACACCA AACTCAGTGC CAGCCCCCAA CTGCCTGCGA CCGACTCCTT CACCTCCCTC 
GCCAGCGCCG ACAGCATGAT CCCTCCAGCG GAGGATGCGG CCGCCCAATC AGATGCCCAG 
TCAGACTCCC AGTTGGAAGG CTCGTCCAGT CAGACGGTGA CTCCTCTGTC GGAGATGGCT 
GGCTCCGCCC TCCTGGACTC CCTTCCTGCC CTGGATTTAT TGCCCAGTGA AGAACTGCCC 
ACTGACCCCA GCAACAACTC CACCCCCAGC TCCACGCAGG ACGAGGAAGC CAGTACAGCG 
CCTAAAGACA CCCCCTCGTC GGACCTGACC GGCAGGCTAC ATATCAATGC CGTGTATCAC 
ATCAGCGCAG AGCACCTACA GCACGCACTG ACCACCGACA CCCAATTCAT GAACGATTTC 
ATGGAGCAGC GGAAATTCAC AGATATCACA GTGAATCCCT GGATGCGGGA CGGCAACGGG 
AAACAGTCTC GGATACTGAA TTACACAATC CCCATCAATA ACCCTCTGGG GCCCAAATCT 
GCCCCGGCTA TAGAAACCCA GATTCTGCAC AGTGTGAAGG GGTCAGTCTG TGTTCTCGAC 
ACCCAGGTTA TAACTCAGGG CATCCCCTAC CAGGATTATT TCTACACCTC TCACCGGTAC 
TGCATCAGCA GCGTGGGCAA GAACAAGGCA CGCCTCAGGG TTTCCTCTGA GATCTGCTAC 
AGGAAGCAGC CATGGAGCCT GGTACGAGCC ATCATAGAGA AGAACTCCTG GAATGGCATG 
GAGGAGCACT TCAGCCTGCT GGCCGATGCG GTGGCAAAGC TGGACCAGTC CTGCGCGGAG 
GAGATGGGGG GCAGAGAAAA GCCTGCGCTA CGGCGGAGGA AGCGCACTTT AAGCTGGAAG 
TCCCATCACA ACGATACCAA TAACACCGCC TCCCCCCAGA TTGAAAGCGA AAGAGTTTCC 
CGTAACTCCA GAGTTTCAGG CAGCATCTCC TCTCAGCTCT CCCAGCTCTC CAACCGAGAC 
ACGTCCAGCA ACATCCCCAA CGCGCTGTTT GTTATCAGTA TTGTGCTGCT GGTCCTGGTG 
GTCCTGAACA TGATGCTCTT CTACCGCCTC TGGTCGCTGG AGCACACGGC TCGGACATTC 
CAGACGTGGC AAAACTACGC CGTGTCCCAA GGGAGGTTCC CCCAGAGTTC CGCCGACTGG 
GCCGAGATCC TCGAGCTACA GAAGCGCTTC CACAACGCCG AGGTGCAGAA ATGGAAGCAG 
ACGCTCCGAG CCTCTGTGCA GCTGCTGGAT GAGATGAGGA TGTCTCTGCA GAGACTGCAG 
CAGGGAGTCC TGATGGCCGA TTCCGACACA GAACCTCACG AGGAAGACGC CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1a ( XM_031905433.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761293.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa61775
Clone ID Related Accession (Same CDS sequence)	XM_031905433.1
Accession Version	XM_031905433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGCC TGGCCTGTCA GGGATGGAGC GCCACTTTCC TGAGCACTGG CAGCATCACT 
CCCCGCAGCA CTCCCAGTAA CTCCCCTGCT GTGCGGAGGC GTCTGGTGAG CAGCGAAGTT 
GACAATATGG TGGAAAGGGG ATCCGAAGGC TCCGCAGAGC AGTCCCCCAA CACTTTAACC 
CCCACGCTAC CCCAGTCTGC CGCTGTGCCA GGGACCCAGG GCAATGCCCA GCAACCCACG 
CAGTCCAACT CCCAGCCTGG AACCCCACTC ACGGCGCAGC TCAGCGGGCG AGGATTCATA 
CGGAACAGCA AGAAGATGCA GAGCTGGTAC AGTATGCTGA GCCCCACGTA CAAGCAGCGC 
AACGAAGACT TTCGCAAGAT TTTCAAGAAG CTTCCGGATT CCGAGCGCCT CATTGTTGAC 
TACTCCTGTG CGCTGCAGAA GGATATCTTA CTCCAGGGAA GGCTCTATTT GTCGGAGAAC 
TGGATCTGTT TTTATAGCAA CATTTTCCGC TGGGAAACCA CGATCATGAT CCAGCTGAAG 
GACATACAAT GCATCAAGAA GGAGAAGACA GCCAAGCTGA TTCCCAACGC CATCCAAGTA 
TGCACTGAAA CCGAGAAGCA CTTTTTTACC TCCTTCGGCG CCCGAGATCG GTCGTTTCTG 
CTGATTTTCC GTCTCTGGCA GAATGCTCTT CTAGATAAGA CCCTGTCCCC GCGGGAGCTG 
TGGCACATTG TACACCAGTG TTACGGGACT GAGCTGGGAC TGACCAGTGA GGATGAAGAT 
TATGTGTCGC CTCGAGACCT GCTGAACGGC TTGGGGGGGA GTGAGGATTT CCCTGAGAGC 
ACCGACCTGA GTGACACTTC ATCCCGCGGT GACACCAAAC TCAGTGCCAG CCCCCAACTG 
CCTGCGACCG ACTCCTTCAC CTCCCTCGCC AGCGCCGACA GCATGATCCC TCCAGCGGAG 
GATGCGGCCG CCCAATCAGA TGCCCAGTCA GACTCCCAGT TGGAAGGCTC GTCCAGTCAG 
ACGGTGACTC CTCTGTCGGA GATGGCTGGC TCCGCCCTCC TGGACTCCCT TCCTGCCCTG 
GATTTATTGC CCAGTGAAGA ACTGCCCACT GACCCCAGCA ACAACTCCAC CCCCAGCTCC 
ACGCAGGACG AGGAAGCCAG TACAGCGCCT AAAGACACCC CCTCGTCGGA CCTGACCGGC 
AGGCTACATA TCAATGCCGT GTATCACATC AGCGCAGAGC ACCTACAGCA CGCACTGACC 
ACCGACACCC AATTCATGAA CGATTTCATG GAGCAGCGGA AATTCACAGA TATCACAGTG 
AATCCCTGGA TGCGGGACGG CAACGGGAAA CAGTCTCGGA TACTGAATTA CACAATCCCC 
ATCAATAACC CTCTGGGGCC CAAATCTGCC CCGGCTATAG AAACCCAGAT TCTGCACAGT 
GTGAAGGGGT CAGTCTGTGT TCTCGACACC CAGGTTATAA CTCAGGGCAT CCCCTACCAG 
GATTATTTCT ACACCTCTCA CCGGTACTGC ATCAGCAGCG TGGGCAAGAA CAAGGCACGC 
CTCAGGGTTT CCTCTGAGAT CTGCTACAGG AAGCAGCCAT GGAGCCTGGT ACGAGCCATC 
ATAGAGAAGA ACTCCTGGAA TGGCATGGAG GAGCACTTCA GCCTGCTGGC CGATGCGGTG 
GCAAAGCTGG ACCAGTCCTG CGCGGAGGAG ATGGGGGGCA GAGAAAAGCC TGCGCTACGG 
CGGAGGAAGC GCACTTTAAG CTGGAAGTCC CATCACAACG ATACCAATAA CACCGCCTCC 
CCCCAGATTG AAAGCGAAAG AGTTTCCCGT AACTCCAGAG TTTCAGGCAG CATCTCCTCT 
CAGCTCTCCC AGCTCTCCAA CCGAGACACG TCCAGCAACA TCCCCAACGC GCTGTTTGTT 
ATCAGTATTG TGCTGCTGGT CCTGGTGGTC CTGAACATGA TGCTCTTCTA CCGCCTCTGG 
TCGCTGGAGC ACACGGCTCG GACATTCCAG ACGTGGCAAA ACTACGCCGT GTCCCAAGGG 
AGGTTCCCCC AGAGTTCCGC CGACTGGGCC GAGATCCTCG AGCTACAGAA GCGCTTCCAC 
AACGCCGAGG TGCAGAAATG GAAGCAGACG CTCCGAGCCT CTGTGCAGCT GCTGGATGAG 
ATGAGGATGT CTCTGCAGAG ACTGCAGCAG GGAGTCCTGA TGGCCGATTC CGACACAGAA 
CCTCACGAGG AAGACGCCCT GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761293.1
CDS	167..2413
Translation	MECLACQGWSATFLSTGSITPRSTPSNSPAVRRRLVSSEVDNMVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKKMQSWYSMLSPTYKQRNEDFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTIMIQLKDIQCIKKEKTAKLIPNAIQVCTETEKHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTAPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPQSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_031905433.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905433.1

ORF Insert Sequence:

ATGGAGTGCC TGGCCTGTCA GGGATGGAGC GCCACTTTCC TGAGCACTGG CAGCATCACT 
CCCCGCAGCA CTCCCAGTAA CTCCCCTGCT GTGCGGAGGC GTCTGGTGAG CAGCGAAGTT 
GACAATATGG TGGAAAGGGG ATCCGAAGGC TCCGCAGAGC AGTCCCCCAA CACTTTAACC 
CCCACGCTAC CCCAGTCTGC CGCTGTGCCA GGGACCCAGG GCAATGCCCA GCAACCCACG 
CAGTCCAACT CCCAGCCTGG AACCCCACTC ACGGCGCAGC TCAGCGGGCG AGGATTCATA 
CGGAACAGCA AGAAGATGCA GAGCTGGTAC AGTATGCTGA GCCCCACGTA CAAGCAGCGC 
AACGAAGACT TTCGCAAGAT TTTCAAGAAG CTTCCGGATT CCGAGCGCCT CATTGTTGAC 
TACTCCTGTG CGCTGCAGAA GGATATCTTA CTCCAGGGAA GGCTCTATTT GTCGGAGAAC 
TGGATCTGTT TTTATAGCAA CATTTTCCGC TGGGAAACCA CGATCATGAT CCAGCTGAAG 
GACATACAAT GCATCAAGAA GGAGAAGACA GCCAAGCTGA TTCCCAACGC CATCCAAGTA 
TGCACTGAAA CCGAGAAGCA CTTTTTTACC TCCTTCGGCG CCCGAGATCG GTCGTTTCTG 
CTGATTTTCC GTCTCTGGCA GAATGCTCTT CTAGATAAGA CCCTGTCCCC GCGGGAGCTG 
TGGCACATTG TACACCAGTG TTACGGGACT GAGCTGGGAC TGACCAGTGA GGATGAAGAT 
TATGTGTCGC CTCGAGACCT GCTGAACGGC TTGGGGGGGA GTGAGGATTT CCCTGAGAGC 
ACCGACCTGA GTGACACTTC ATCCCGCGGT GACACCAAAC TCAGTGCCAG CCCCCAACTG 
CCTGCGACCG ACTCCTTCAC CTCCCTCGCC AGCGCCGACA GCATGATCCC TCCAGCGGAG 
GATGCGGCCG CCCAATCAGA TGCCCAGTCA GACTCCCAGT TGGAAGGCTC GTCCAGTCAG 
ACGGTGACTC CTCTGTCGGA GATGGCTGGC TCCGCCCTCC TGGACTCCCT TCCTGCCCTG 
GATTTATTGC CCAGTGAAGA ACTGCCCACT GACCCCAGCA ACAACTCCAC CCCCAGCTCC 
ACGCAGGACG AGGAAGCCAG TACAGCGCCT AAAGACACCC CCTCGTCGGA CCTGACCGGC 
AGGCTACATA TCAATGCCGT GTATCACATC AGCGCAGAGC ACCTACAGCA CGCACTGACC 
ACCGACACCC AATTCATGAA CGATTTCATG GAGCAGCGGA AATTCACAGA TATCACAGTG 
AATCCCTGGA TGCGGGACGG CAACGGGAAA CAGTCTCGGA TACTGAATTA CACAATCCCC 
ATCAATAACC CTCTGGGGCC CAAATCTGCC CCGGCTATAG AAACCCAGAT TCTGCACAGT 
GTGAAGGGGT CAGTCTGTGT TCTCGACACC CAGGTTATAA CTCAGGGCAT CCCCTACCAG 
GATTATTTCT ACACCTCTCA CCGGTACTGC ATCAGCAGCG TGGGCAAGAA CAAGGCACGC 
CTCAGGGTTT CCTCTGAGAT CTGCTACAGG AAGCAGCCAT GGAGCCTGGT ACGAGCCATC 
ATAGAGAAGA ACTCCTGGAA TGGCATGGAG GAGCACTTCA GCCTGCTGGC CGATGCGGTG 
GCAAAGCTGG ACCAGTCCTG CGCGGAGGAG ATGGGGGGCA GAGAAAAGCC TGCGCTACGG 
CGGAGGAAGC GCACTTTAAG CTGGAAGTCC CATCACAACG ATACCAATAA CACCGCCTCC 
CCCCAGATTG AAAGCGAAAG AGTTTCCCGT AACTCCAGAG TTTCAGGCAG CATCTCCTCT 
CAGCTCTCCC AGCTCTCCAA CCGAGACACG TCCAGCAACA TCCCCAACGC GCTGTTTGTT 
ATCAGTATTG TGCTGCTGGT CCTGGTGGTC CTGAACATGA TGCTCTTCTA CCGCCTCTGG 
TCGCTGGAGC ACACGGCTCG GACATTCCAG ACGTGGCAAA ACTACGCCGT GTCCCAAGGG 
AGGTTCCCCC AGAGTTCCGC CGACTGGGCC GAGATCCTCG AGCTACAGAA GCGCTTCCAC 
AACGCCGAGG TGCAGAAATG GAAGCAGACG CTCCGAGCCT CTGTGCAGCT GCTGGATGAG 
ATGAGGATGT CTCTGCAGAG ACTGCAGCAG GGAGTCCTGA TGGCCGATTC CGACACAGAA 
CCTCACGAGG AAGACGCCCT GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1a ( XM_031905434.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761294.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa61776
Clone ID Related Accession (Same CDS sequence)	XM_031905434.1
Accession Version	XM_031905434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGACA CTGGCAGCAT CACTCCCCGC AGCACTCCCA GTAACTCCCC TGCTGTGCGG 
AGGCGTCTGG TGAGCAGCGA AGTTGACAAT ATGGTGGAAA GGGGATCCGA AGGCTCCGCA 
GAGCAGTCCC CCAACACTTT AACCCCCACG CTACCCCAGT CTGCCGCTGT GCCAGGGACC 
CAGGGCAATG CCCAGCAACC CACGCAGTCC AACTCCCAGC CTGGAACCCC ACTCACGGCG 
CAGCTCAGCG GGCGAGGATT CATACGGAAC AGCAAGAAGA TGCAGAGCTG GTACAGTATG 
CTGAGCCCCA CGTACAAGCA GCGCAACGAA GACTTTCGCA AGATTTTCAA GAAGCTTCCG 
GATTCCGAGC GCCTCATTGT TGACTACTCC TGTGCGCTGC AGAAGGATAT CTTACTCCAG 
GGAAGGCTCT ATTTGTCGGA GAACTGGATC TGTTTTTATA GCAACATTTT CCGCTGGGAA 
ACCACGATCA TGATCCAGCT GAAGGACATA CAATGCATCA AGAAGGAGAA GACAGCCAAG 
CTGATTCCCA ACGCCATCCA AGTATGCACT GAAACCGAGA AGCACTTTTT TACCTCCTTC 
GGCGCCCGAG ATCGGTCGTT TCTGCTGATT TTCCGTCTCT GGCAGAATGC TCTTCTAGAT 
AAGACCCTGT CCCCGCGGGA GCTGTGGCAC ATTGTACACC AGTGTTACGG GACTGAGCTG 
GGACTGACCA GTGAGGATGA AGATTATGTG TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG 
GGGAGTGAGG ATTTCCCTGA GAGCACCGAC CTGAGTGACA CTTCATCCCG CGGTGACACC 
AAACTCAGTG CCAGCCCCCA ACTGCCTGCG ACCGACTCCT TCACCTCCCT CGCCAGCGCC 
GACAGCATGA TCCCTCCAGC GGAGGATGCG GCCGCCCAAT CAGATGCCCA GTCAGACTCC 
CAGTTGGAAG GCTCGTCCAG TCAGACGGTG ACTCCTCTGT CGGAGATGGC TGGCTCCGCC 
CTCCTGGACT CCCTTCCTGC CCTGGATTTA TTGCCCAGTG AAGAACTGCC CACTGACCCC 
AGCAACAACT CCACCCCCAG CTCCACGCAG GACGAGGAAG CCAGTACAGC GCCTAAAGAC 
ACCCCCTCGT CGGACCTGAC CGGCAGGCTA CATATCAATG CCGTGTATCA CATCAGCGCA 
GAGCACCTAC AGCACGCACT GACCACCGAC ACCCAATTCA TGAACGATTT CATGGAGCAG 
CGGAAATTCA CAGATATCAC AGTGAATCCC TGGATGCGGG ACGGCAACGG GAAACAGTCT 
CGGATACTGA ATTACACAAT CCCCATCAAT AACCCTCTGG GGCCCAAATC TGCCCCGGCT 
ATAGAAACCC AGATTCTGCA CAGTGTGAAG GGGTCAGTCT GTGTTCTCGA CACCCAGGTT 
ATAACTCAGG GCATCCCCTA CCAGGATTAT TTCTACACCT CTCACCGGTA CTGCATCAGC 
AGCGTGGGCA AGAACAAGGC ACGCCTCAGG GTTTCCTCTG AGATCTGCTA CAGGAAGCAG 
CCATGGAGCC TGGTACGAGC CATCATAGAG AAGAACTCCT GGAATGGCAT GGAGGAGCAC 
TTCAGCCTGC TGGCCGATGC GGTGGCAAAG CTGGACCAGT CCTGCGCGGA GGAGATGGGG 
GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG AAGCGCACTT TAAGCTGGAA GTCCCATCAC 
AACGATACCA ATAACACCGC CTCCCCCCAG ATTGAAAGCG AAAGAGTTTC CCGTAACTCC 
AGAGTTTCAG GCAGCATCTC CTCTCAGCTC TCCCAGCTCT CCAACCGAGA CACGTCCAGC 
AACATCCCCA ACGCGCTGTT TGTTATCAGT ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC 
ATGATGCTCT TCTACCGCCT CTGGTCGCTG GAGCACACGG CTCGGACATT CCAGACGTGG 
CAAAACTACG CCGTGTCCCA AGGGAGGTTC CCCCAGAGTT CCGCCGACTG GGCCGAGATC 
CTCGAGCTAC AGAAGCGCTT CCACAACGCC GAGGTGCAGA AATGGAAGCA GACGCTCCGA 
GCCTCTGTGC AGCTGCTGGA TGAGATGAGG ATGTCTCTGC AGAGACTGCA GCAGGGAGTC 
CTGATGGCCG ATTCCGACAC AGAACCTCAC GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761294.1
CDS	683..2893
Translation	MIDTGSITPRSTPSNSPAVRRRLVSSEVDNMVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKKMQSWYSMLSPTYKQRNEDFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTIMIQLKDIQCIKKEKTAKLIPNAIQVCTETEKHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTAPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPQSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_031905434.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905434.1

ORF Insert Sequence:

ATGATTGACA CTGGCAGCAT CACTCCCCGC AGCACTCCCA GTAACTCCCC TGCTGTGCGG 
AGGCGTCTGG TGAGCAGCGA AGTTGACAAT ATGGTGGAAA GGGGATCCGA AGGCTCCGCA 
GAGCAGTCCC CCAACACTTT AACCCCCACG CTACCCCAGT CTGCCGCTGT GCCAGGGACC 
CAGGGCAATG CCCAGCAACC CACGCAGTCC AACTCCCAGC CTGGAACCCC ACTCACGGCG 
CAGCTCAGCG GGCGAGGATT CATACGGAAC AGCAAGAAGA TGCAGAGCTG GTACAGTATG 
CTGAGCCCCA CGTACAAGCA GCGCAACGAA GACTTTCGCA AGATTTTCAA GAAGCTTCCG 
GATTCCGAGC GCCTCATTGT TGACTACTCC TGTGCGCTGC AGAAGGATAT CTTACTCCAG 
GGAAGGCTCT ATTTGTCGGA GAACTGGATC TGTTTTTATA GCAACATTTT CCGCTGGGAA 
ACCACGATCA TGATCCAGCT GAAGGACATA CAATGCATCA AGAAGGAGAA GACAGCCAAG 
CTGATTCCCA ACGCCATCCA AGTATGCACT GAAACCGAGA AGCACTTTTT TACCTCCTTC 
GGCGCCCGAG ATCGGTCGTT TCTGCTGATT TTCCGTCTCT GGCAGAATGC TCTTCTAGAT 
AAGACCCTGT CCCCGCGGGA GCTGTGGCAC ATTGTACACC AGTGTTACGG GACTGAGCTG 
GGACTGACCA GTGAGGATGA AGATTATGTG TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG 
GGGAGTGAGG ATTTCCCTGA GAGCACCGAC CTGAGTGACA CTTCATCCCG CGGTGACACC 
AAACTCAGTG CCAGCCCCCA ACTGCCTGCG ACCGACTCCT TCACCTCCCT CGCCAGCGCC 
GACAGCATGA TCCCTCCAGC GGAGGATGCG GCCGCCCAAT CAGATGCCCA GTCAGACTCC 
CAGTTGGAAG GCTCGTCCAG TCAGACGGTG ACTCCTCTGT CGGAGATGGC TGGCTCCGCC 
CTCCTGGACT CCCTTCCTGC CCTGGATTTA TTGCCCAGTG AAGAACTGCC CACTGACCCC 
AGCAACAACT CCACCCCCAG CTCCACGCAG GACGAGGAAG CCAGTACAGC GCCTAAAGAC 
ACCCCCTCGT CGGACCTGAC CGGCAGGCTA CATATCAATG CCGTGTATCA CATCAGCGCA 
GAGCACCTAC AGCACGCACT GACCACCGAC ACCCAATTCA TGAACGATTT CATGGAGCAG 
CGGAAATTCA CAGATATCAC AGTGAATCCC TGGATGCGGG ACGGCAACGG GAAACAGTCT 
CGGATACTGA ATTACACAAT CCCCATCAAT AACCCTCTGG GGCCCAAATC TGCCCCGGCT 
ATAGAAACCC AGATTCTGCA CAGTGTGAAG GGGTCAGTCT GTGTTCTCGA CACCCAGGTT 
ATAACTCAGG GCATCCCCTA CCAGGATTAT TTCTACACCT CTCACCGGTA CTGCATCAGC 
AGCGTGGGCA AGAACAAGGC ACGCCTCAGG GTTTCCTCTG AGATCTGCTA CAGGAAGCAG 
CCATGGAGCC TGGTACGAGC CATCATAGAG AAGAACTCCT GGAATGGCAT GGAGGAGCAC 
TTCAGCCTGC TGGCCGATGC GGTGGCAAAG CTGGACCAGT CCTGCGCGGA GGAGATGGGG 
GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG AAGCGCACTT TAAGCTGGAA GTCCCATCAC 
AACGATACCA ATAACACCGC CTCCCCCCAG ATTGAAAGCG AAAGAGTTTC CCGTAACTCC 
AGAGTTTCAG GCAGCATCTC CTCTCAGCTC TCCCAGCTCT CCAACCGAGA CACGTCCAGC 
AACATCCCCA ACGCGCTGTT TGTTATCAGT ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC 
ATGATGCTCT TCTACCGCCT CTGGTCGCTG GAGCACACGG CTCGGACATT CCAGACGTGG 
CAAAACTACG CCGTGTCCCA AGGGAGGTTC CCCCAGAGTT CCGCCGACTG GGCCGAGATC 
CTCGAGCTAC AGAAGCGCTT CCACAACGCC GAGGTGCAGA AATGGAAGCA GACGCTCCGA 
GCCTCTGTGC AGCTGCTGGA TGAGATGAGG ATGTCTCTGC AGAGACTGCA GCAGGGAGTC 
CTGATGGCCG ATTCCGACAC AGAACCTCAC GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gramd1a ( XM_031905435.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761295.1 Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X5, mRNA.

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CloneID	OXa61777
Clone ID Related Accession (Same CDS sequence)	XM_031905435.1
Accession Version	XM_031905435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein Aster-A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGAAGA TGCAGAGCTG GTACAGTATG CTGAGCCCCA CGTACAAGCA GCGCAACGAA 
GACTTTCGCA AGATTTTCAA GAAGCTTCCG GATTCCGAGC GCCTCATTGT TGACTACTCC 
TGTGCGCTGC AGAAGGATAT CTTACTCCAG GGAAGGCTCT ATTTGTCGGA GAACTGGATC 
TGTTTTTATA GCAACATTTT CCGCTGGGAA ACCACGATCA TGATCCAGCT GAAGGACATA 
CAATGCATCA AGAAGGAGAA GACAGCCAAG CTGATTCCCA ACGCCATCCA AGTATGCACT 
GAAACCGAGA AGCACTTTTT TACCTCCTTC GGCGCCCGAG ATCGGTCGTT TCTGCTGATT 
TTCCGTCTCT GGCAGAATGC TCTTCTAGAT AAGACCCTGT CCCCGCGGGA GCTGTGGCAC 
ATTGTACACC AGTGTTACGG GACTGAGCTG GGACTGACCA GTGAGGATGA AGATTATGTG 
TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG GGGAGTGAGG ATTTCCCTGA GAGCACCGAC 
CTGAGTGACA CTTCATCCCG CGGTGACACC AAACTCAGTG CCAGCCCCCA ACTGCCTGCG 
ACCGACTCCT TCACCTCCCT CGCCAGCGCC GACAGCATGA TCCCTCCAGC GGAGGATGCG 
GCCGCCCAAT CAGATGCCCA GTCAGACTCC CAGTTGGAAG GCTCGTCCAG TCAGACGGTG 
ACTCCTCTGT CGGAGATGGC TGGCTCCGCC CTCCTGGACT CCCTTCCTGC CCTGGATTTA 
TTGCCCAGTG AAGAACTGCC CACTGACCCC AGCAACAACT CCACCCCCAG CTCCACGCAG 
GACGAGGAAG CCAGTACAGC GCCTAAAGAC ACCCCCTCGT CGGACCTGAC CGGCAGGCTA 
CATATCAATG CCGTGTATCA CATCAGCGCA GAGCACCTAC AGCACGCACT GACCACCGAC 
ACCCAATTCA TGAACGATTT CATGGAGCAG CGGAAATTCA CAGATATCAC AGTGAATCCC 
TGGATGCGGG ACGGCAACGG GAAACAGTCT CGGATACTGA ATTACACAAT CCCCATCAAT 
AACCCTCTGG GGCCCAAATC TGCCCCGGCT ATAGAAACCC AGATTCTGCA CAGTGTGAAG 
GGGTCAGTCT GTGTTCTCGA CACCCAGGTT ATAACTCAGG GCATCCCCTA CCAGGATTAT 
TTCTACACCT CTCACCGGTA CTGCATCAGC AGCGTGGGCA AGAACAAGGC ACGCCTCAGG 
GTTTCCTCTG AGATCTGCTA CAGGAAGCAG CCATGGAGCC TGGTACGAGC CATCATAGAG 
AAGAACTCCT GGAATGGCAT GGAGGAGCAC TTCAGCCTGC TGGCCGATGC GGTGGCAAAG 
CTGGACCAGT CCTGCGCGGA GGAGATGGGG GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG 
AAGCGCACTT TAAGCTGGAA GTCCCATCAC AACGATACCA ATAACACCGC CTCCCCCCAG 
ATTGAAAGCG AAAGAGTTTC CCGTAACTCC AGAGTTTCAG GCAGCATCTC CTCTCAGCTC 
TCCCAGCTCT CCAACCGAGA CACGTCCAGC AACATCCCCA ACGCGCTGTT TGTTATCAGT 
ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC ATGATGCTCT TCTACCGCCT CTGGTCGCTG 
GAGCACACGG CTCGGACATT CCAGACGTGG CAAAACTACG CCGTGTCCCA AGGGAGGTTC 
CCCCAGAGTT CCGCCGACTG GGCCGAGATC CTCGAGCTAC AGAAGCGCTT CCACAACGCC 
GAGGTGCAGA AATGGAAGCA GACGCTCCGA GCCTCTGTGC AGCTGCTGGA TGAGATGAGG 
ATGTCTCTGC AGAGACTGCA GCAGGGAGTC CTGATGGCCG ATTCCGACAC AGAACCTCAC 
GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761295.1
CDS	203..2323
Translation	MVERGSEGSAEQSPNTLTPTLPQSAAVPGTQGNAQQPTQSNSQPGTPLTAQLSGRGFIRNSKKMQSWYSMLSPTYKQRNEDFRKIFKKLPDSERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTIMIQLKDIQCIKKEKTAKLIPNAIQVCTETEKHFFTSFGARDRSFLLIFRLWQNALLDKTLSPRELWHIVHQCYGTELGLTSEDEDYVSPRDLLNGLGGSEDFPESTDLSDTSSRGDTKLSASPQLPATDSFTSLASADSMIPPAEDAAAQSDAQSDSQLEGSSSQTVTPLSEMAGSALLDSLPALDLLPSEELPTDPSNNSTPSSTQDEEASTAPKDTPSSDLTGRLHINAVYHISAEHLQHALTTDTQFMNDFMEQRKFTDITVNPWMRDGNGKQSRILNYTIPINNPLGPKSAPAIETQILHSVKGSVCVLDTQVITQGIPYQDYFYTSHRYCISSVGKNKARLRVSSEICYRKQPWSLVRAIIEKNSWNGMEEHFSLLADAVAKLDQSCAEEMGGREKPALRRRKRTLSWKSHHNDTNNTASPQIESERVSRNSRVSGSISSQLSQLSNRDTSSNIPNALFVISIVLLVLVVLNMMLFYRLWSLEHTARTFQTWQNYAVSQGRFPQSSADWAEILELQKRFHNAEVQKWKQTLRASVQLLDEMRMSLQRLQQGVLMADSDTEPHEEDALS

Target ORF information:

RefSeq Version	XM_031905435.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GRAM domain containing 1A (gramd1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905435.1

ORF Insert Sequence:

ATGGTGGAAA GGGGATCCGA AGGCTCCGCA GAGCAGTCCC CCAACACTTT AACCCCCACG 
CTACCCCAGT CTGCCGCTGT GCCAGGGACC CAGGGCAATG CCCAGCAACC CACGCAGTCC 
AACTCCCAGC CTGGAACCCC ACTCACGGCG CAGCTCAGCG GGCGAGGATT CATACGGAAC 
AGCAAGAAGA TGCAGAGCTG GTACAGTATG CTGAGCCCCA CGTACAAGCA GCGCAACGAA 
GACTTTCGCA AGATTTTCAA GAAGCTTCCG GATTCCGAGC GCCTCATTGT TGACTACTCC 
TGTGCGCTGC AGAAGGATAT CTTACTCCAG GGAAGGCTCT ATTTGTCGGA GAACTGGATC 
TGTTTTTATA GCAACATTTT CCGCTGGGAA ACCACGATCA TGATCCAGCT GAAGGACATA 
CAATGCATCA AGAAGGAGAA GACAGCCAAG CTGATTCCCA ACGCCATCCA AGTATGCACT 
GAAACCGAGA AGCACTTTTT TACCTCCTTC GGCGCCCGAG ATCGGTCGTT TCTGCTGATT 
TTCCGTCTCT GGCAGAATGC TCTTCTAGAT AAGACCCTGT CCCCGCGGGA GCTGTGGCAC 
ATTGTACACC AGTGTTACGG GACTGAGCTG GGACTGACCA GTGAGGATGA AGATTATGTG 
TCGCCTCGAG ACCTGCTGAA CGGCTTGGGG GGGAGTGAGG ATTTCCCTGA GAGCACCGAC 
CTGAGTGACA CTTCATCCCG CGGTGACACC AAACTCAGTG CCAGCCCCCA ACTGCCTGCG 
ACCGACTCCT TCACCTCCCT CGCCAGCGCC GACAGCATGA TCCCTCCAGC GGAGGATGCG 
GCCGCCCAAT CAGATGCCCA GTCAGACTCC CAGTTGGAAG GCTCGTCCAG TCAGACGGTG 
ACTCCTCTGT CGGAGATGGC TGGCTCCGCC CTCCTGGACT CCCTTCCTGC CCTGGATTTA 
TTGCCCAGTG AAGAACTGCC CACTGACCCC AGCAACAACT CCACCCCCAG CTCCACGCAG 
GACGAGGAAG CCAGTACAGC GCCTAAAGAC ACCCCCTCGT CGGACCTGAC CGGCAGGCTA 
CATATCAATG CCGTGTATCA CATCAGCGCA GAGCACCTAC AGCACGCACT GACCACCGAC 
ACCCAATTCA TGAACGATTT CATGGAGCAG CGGAAATTCA CAGATATCAC AGTGAATCCC 
TGGATGCGGG ACGGCAACGG GAAACAGTCT CGGATACTGA ATTACACAAT CCCCATCAAT 
AACCCTCTGG GGCCCAAATC TGCCCCGGCT ATAGAAACCC AGATTCTGCA CAGTGTGAAG 
GGGTCAGTCT GTGTTCTCGA CACCCAGGTT ATAACTCAGG GCATCCCCTA CCAGGATTAT 
TTCTACACCT CTCACCGGTA CTGCATCAGC AGCGTGGGCA AGAACAAGGC ACGCCTCAGG 
GTTTCCTCTG AGATCTGCTA CAGGAAGCAG CCATGGAGCC TGGTACGAGC CATCATAGAG 
AAGAACTCCT GGAATGGCAT GGAGGAGCAC TTCAGCCTGC TGGCCGATGC GGTGGCAAAG 
CTGGACCAGT CCTGCGCGGA GGAGATGGGG GGCAGAGAAA AGCCTGCGCT ACGGCGGAGG 
AAGCGCACTT TAAGCTGGAA GTCCCATCAC AACGATACCA ATAACACCGC CTCCCCCCAG 
ATTGAAAGCG AAAGAGTTTC CCGTAACTCC AGAGTTTCAG GCAGCATCTC CTCTCAGCTC 
TCCCAGCTCT CCAACCGAGA CACGTCCAGC AACATCCCCA ACGCGCTGTT TGTTATCAGT 
ATTGTGCTGC TGGTCCTGGT GGTCCTGAAC ATGATGCTCT TCTACCGCCT CTGGTCGCTG 
GAGCACACGG CTCGGACATT CCAGACGTGG CAAAACTACG CCGTGTCCCA AGGGAGGTTC 
CCCCAGAGTT CCGCCGACTG GGCCGAGATC CTCGAGCTAC AGAAGCGCTT CCACAACGCC 
GAGGTGCAGA AATGGAAGCA GACGCTCCGA GCCTCTGTGC AGCTGCTGGA TGAGATGAGG 
ATGTCTCTGC AGAGACTGCA GCAGGGAGTC CTGATGGCCG ATTCCGACAC AGAACCTCAC 
GAGGAAGACG CCCTGTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gramd1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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