nalcn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nalcn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nalcn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa52534 XM_012957711.3
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52534 XM_012957710.3
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52534 XM_002937079.5
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa52535 XM_012957712.3
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa05901 XM_012957710.2
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa05901 XM_002937079.4
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa05901 XM_012957711.2
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa35339 XM_012957712.2
Latest version!
Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa52534
    Clone ID Related Accession (Same CDS sequence) XM_012957711.3 , XM_002937079.5 , XM_012957710.3
    Accession Version XM_012957711.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813165.1
    CDS349..5583
    Translation

    Target ORF information:

    RefSeq Version XM_012957711.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957711.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52534
    Clone ID Related Accession (Same CDS sequence) XM_012957711.3 , XM_002937079.5 , XM_012957710.3
    Accession Version XM_002937079.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937079.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937125.2
    CDS549..5783
    Translation

    Target ORF information:

    RefSeq Version XM_002937079.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937079.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52534
    Clone ID Related Accession (Same CDS sequence) XM_012957711.3 , XM_002937079.5 , XM_012957710.3
    Accession Version XM_012957710.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813164.1
    CDS384..5618
    Translation

    Target ORF information:

    RefSeq Version XM_012957710.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957710.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TTATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CGCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa52535
    Clone ID Related Accession (Same CDS sequence) XM_012957712.3
    Accession Version XM_012957712.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
    GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT
    TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA
    TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT
    CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT
    TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA
    TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC
    ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT
    GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT
    GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT
    GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT
    AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT
    GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT
    GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA
    TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT
    TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT
    ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT
    ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA
    GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG
    AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT
    GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC
    GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC
    TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT
    ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG
    GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA
    CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT
    GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA
    AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT
    CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT
    TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT
    CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC
    GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG
    CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT
    TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT
    CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG
    TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA
    AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA
    TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA
    CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT
    CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA
    GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG
    CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC
    CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA
    AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG
    TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA
    TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA
    GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATTAT GGCAGATGGC
    TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT
    TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA
    ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA
    CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT
    ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG
    AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT
    AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG
    CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA
    GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA
    GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT
    CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG
    AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA
    CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC
    CGCATGTATG ACATAACGCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA
    GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG
    GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA
    GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA
    TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG
    ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG
    AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA
    ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA
    TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG
    CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG
    CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA
    GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA
    CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC
    CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA
    GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG
    GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA
    CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA
    GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC
    ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA
    CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA
    CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA
    GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT
    CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA
    CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT
    GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG
    ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA
    CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813166.1
    CDS443..5653
    Translation

    Target ORF information:

    RefSeq Version XM_012957712.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957712.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
    GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT
    TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA
    TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT
    CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT
    TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA
    TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC
    ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT
    GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT
    GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT
    GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT
    AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT
    GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT
    GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA
    TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT
    TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT
    ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT
    ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA
    GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG
    AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT
    GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC
    GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC
    TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT
    ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG
    GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA
    CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT
    GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA
    AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT
    CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT
    TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT
    CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC
    GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG
    CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT
    TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT
    CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG
    TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA
    AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA
    TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA
    CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT
    CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA
    GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG
    CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC
    CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA
    AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG
    TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA
    TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA
    GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATTAT GGCAGATGGC
    TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT
    TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA
    ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA
    CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT
    ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG
    AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT
    AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG
    CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA
    GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA
    GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT
    CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG
    AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA
    CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC
    CGCATGTATG ACATAACGCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA
    GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG
    GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA
    GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA
    TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG
    ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG
    AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA
    ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA
    TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG
    CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG
    CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA
    GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA
    CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC
    CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA
    GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG
    GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA
    CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA
    GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC
    ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA
    CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA
    CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA
    GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT
    CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA
    CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT
    GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG
    ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA
    CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05901
    Clone ID Related Accession (Same CDS sequence) XM_002937079.4 , XM_012957711.2 , XM_012957710.2
    Accession Version XM_002937079.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937125.2
    CDS730..5964
    Translation

    Target ORF information:

    RefSeq Version XM_002937079.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937079.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05901
    Clone ID Related Accession (Same CDS sequence) XM_002937079.4 , XM_012957711.2 , XM_012957710.2
    Accession Version XM_012957711.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813165.1
    CDS340..5574
    Translation

    Target ORF information:

    RefSeq Version XM_012957711.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957711.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa05901
    Clone ID Related Accession (Same CDS sequence) XM_002937079.4 , XM_012957711.2 , XM_012957710.2
    Accession Version XM_012957710.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813164.1
    CDS899..6133
    Translation

    Target ORF information:

    RefSeq Version XM_012957710.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957710.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAACCA AAAGTGACAA CAGTATGTTG AAACGAAAGC AAAGCTCAAG AGTGGAAGCA 
    CAGCCAGTCA CAGACTTTGG CCCCGATGAA TCTCTGTCAG ACAATGCTGA TATCCTGTGG
    ATTAACAAAC CATGGGTTCA CTCTTTACTG CGCATCTGCG CAATCATTAG TGTTATTTCT
    GTTTGTATGA ACACCCCAAA GACATTTGAA CATTACCCAC CCCTCCAGTA TGTCACGTTT
    GCACTCGATA CCCTACTTAT GTTTCTTTAC ACCGCAGAAA TGATTGCAAA AATGCACATA
    AGAGGCATTG TAAAGGGTGA TACTTCCTAT GTAAAGGATC GCTGGTGCGT CTTTGATGGA
    TTCATGGTCT TTTGCCTTTG GGTATCATTG GTGTTGCAGG TATTTGAAAT CGCCAAAATC
    GTTGACCAAA TGTCCCCATG GGGCATGCTG CGGATCCCAC GACCTCTGAT AATGATTCGT
    GCCTTCCGTA TATACTTCCG CTTTGAACTC CCAAGAACGA GGATAACAAA TATCTTGAAG
    CGTTCAGGAG AGCAAATTTG GAGTGTTTCC ATATTTTTAT TATTCTTTCT TCTATTATAC
    GGGATTCTCG GAGTTCAGAT GTTTGGAACA TTTACCTTCC ATTGTGTTAC AAATGACACA
    GTACATGGAA ATCTGACTTG GAATAATTTA GCCATTCCAG ATACTCACTG CTCCCCTGAA
    GGTGAAGGAT ACCAGTGTCC TCGTGGTTTT AGGTGCGTGG ATCTGAAAGA ACTTGGACTG
    AGCAGGCAAG AGCTTGGCTA CAGTGGCTTT AATGAAATAG GAACCAGCAT ATTTACGGTT
    TATGAAGCTG CATCTCAAGA AGGATGGGTT TTTCTTATGT ATAGAGCAAT AGACAGCTTC
    CCCAGATGGC GATCGTACTT CTATTTTATT ACCTTAATTT TCTTCCTTGC CTGGCTTGTC
    AAGAATGTCT TCATTGCTGT AATTATTGAA ACATTTGCTG AAATTCGAGT GCAGTTTCAA
    CAGATGTGGG GTTCTCGAAG TAGTACTACC TCAACAGCTA CCACACAGAT GTTTCACGAG
    GATGCAGCAG GGGGCTGGCA GCTAGTGGCA GTTGATGTGA ACAAGCCCCA AGGCAGAGCA
    CCAGCTTGTC TACAGAAAAT GATGAGATCT TCCATTTTCC ACATGTTTAT TCTGAGCATG
    GTTGCTGTGG ATGTAATTGT TGCTGCCAGC AATTACTACA AAGGAGAGAA TTTTAAAAGA
    CAATATGATG AGTTTTATCT GGCCGAGGTG GCATTCACCA TGCTGTTTGA TTTGGAAGCT
    CTTTTAAAGA TTTGGTGCCT GGGCTTTACA GGATATATCA GTTCATCACT ACACAAATTT
    GAGCTTTTGC TTGTAGTTGG AACTACTCTT CATATTTATC CTGATCTTTA CCATTCACAG
    TTTACATACT TTCAGGTTCT AAGGGTGGTG CGACTGATTA AGATTTCTCC TGCCTTAGAA
    GACTTTGTAT ATAAAATATT TGGACCTGGC AAGAAATTAG GAAGTCTTGT GGTTTTCACC
    GCTAGCCTAT TAATTGTAAT GTCTGCCATC AGTTTACAGA TGTTCTGCTT TGTGGAAGAG
    CTGGACCGTT TTACAACATT TCCAAGGGCA TTTATGTCAA TGTTTCAGAT TCTGACACAG
    GAAGGATGGG TGGATGTCAT GGATCAAACA CTTAATGCCG TTGGGCATAT GTGGGCACCA
    GTTGTTGCAA TTTACTTCAT ACTTTATCAT CTTTTTGCTA CTTTGATATT GCTTAGTTTG
    TTTGTGGCTG TTATCCTGGA CAATCTGGAG CTTGATGAAG ACTTGAAAAA GCTTAAACAG
    TTGAAACAAA GTGAAGCAAA TGCCGACACC AAAGAAAAAT TACCACTACG CCTACGTATC
    TTTGAGAAAT TCCCCAACAG ACCGCAAATG GTGAAGATTT CCAAGCTTCC TTCTGATTTC
    ACAGTTCCAA AAATAAGGGA GAGTTTCATG AAGCAGTTTA TTGACCGCCA GCAACAAGAC
    ACAAGCTGTT TATTAAGAAG GCTTCCATCT GCTTCTTCTT CTTCATGTGA TCATTCCAAA
    CGATCAGGAG TAGAAGAGAA TAAGTACATT GACCAAAAGC TTCGTAAATC AGTGTTTAGT
    ATAAGAGCAA GGAATCTTCT GGAAAAGGAA ACTGCAGTTA ATAAAATCCT CAGAGATTGC
    ACAAGACAGC GCATGCTGAG TGGATCCTTT GAAGGACAGC CTGCAAAGGA GAGATCAATA
    CTGAGTGTGC AGCACCACAT AAGACAAGAA CGGAGATCAC TAAGACATGG ATCAAACAGT
    CAGAGAATCA GCAGGGGGAA GTCTCTGGAA ACACTGACAC AAGACCACTC CAATACAGTC
    AGATACAGAA ATGCACAGAG GGAAGACAGT GAGATCAAGA TGATCCAGGA AAAAAAAGAA
    CAAGCAGAAA TGAAAAGGAA AGTGCAGGAA GAAGAGTTAA GAGAAAACCA CCCATACTTT
    GATAAGCCAC TGTTTATAGT TGGCCGTGAA CACCGGTTCC GAAACTTCTG CAGGGTAATA
    GTTCGGGCAC GATTTAATGC CTCAAAGACA GATCCTGTTA CAGGAGCAGT GAAAAACACC
    AAATATCATC AACTTTATGA TTTGTTGGGA CTAGTGACCT ATCTAGACTG GGTTATGATT
    GTTGTTACCA TATGTTCCTG CATATCAATG ATGTTTGAAT CTCCTTTTAG AAGAGTAATG
    CACACCCCCA CGTTACAGAT TGCAGAATAT GTTTTTGTCA TATTCATGAG CATTGAACTG
    AACTTGAAGA TCATGGCAGA TGGCTTATTT TTTACTCCAA CGGCTGTTAT ACGAGACTTT
    GGGGGTGTTA TGGATATCTT CATTTATCTT GTTAGTTTAA TCTTCTTATG CTGGATGCCT
    AATTATGTTC CCGCAAAATC TGGAACCCAA CTATTAATGG TGCTGCGCTG CTTGAGACCA
    CTGAGAATAT TCAAGCTTGT ACCACAGATG AGGAAAGTCG TACGGGAACT TTTCAGTGGG
    TTTAAAGAAA TATTTCTGGT TTCTATTCTT CTGCTGACAC TGATGCTTGT GTTTGCAAGC
    TTTGGCGTGC AGCTCTTTGC AGGGAAACTG GCAAAGTGCA ATGACCCACA CATACTCACC
    AGGGAAGACT GTCATGGAAT ATTTAGAATT AATGTCAGTG TTTCGAAAAA TTTAAATCTG
    AAATTAAAAC CAGAGGAAAA GAAGCCTGGA TTCTGGGTTC CAAGAGTCTG GGCAAATCCC
    CGTAATTTTA ATTTTGACAA TGTAGGAAAT GCCATGCTGG CCTTGTTTGA GGTATTGTCT
    TTGAAGGGCT GGGTTGAAGT GCGAGATGTT ATTATTCATC GAGTTGGACC GATCCATGGA
    ATTTATATTC ATGTCTTTGT GTTTCTTGGC TGTATGATTG GCTTGACTCT CTTTGTTGGT
    GTGGTTATTG CTAACTTTAA TGAGAATAAG GGTACAGCTT TACTGACTGT TGATCAAAGG
    AGATGGGAAG ATCTGAAAAG CAGACTTAAG ATAGCCCAAC CACTTCATCT CCCCCCACGT
    CCAGATAATG ATGGTTTCAG AGCCCGCATG TATGACATAA CTCAACACCC TTTTTTCAAG
    AGGACTATTG CTGTACTTGT TTTAGCACAG TCTGTGCTTC TGTCAGTAAA GTGGGATGTT
    GAAGACCCTG TGACGGTTCC ACTGGCAACT ATGTCTGTGG TTTTCACCTT CATCTTTGTA
    CTTGAGGTAA CCATGAAGAT AACAGCTATG TCACCAGCTG GATTTTGGCA AAGTAGAAGA
    AATCGCTATG ATCTCCTTGT CACATCTCTT GGTGTGATCT GGGTGATACT TCATTTTGCT
    TTACTGAATG AATACACTTA TATGATGGGT GCTTGTATTA TCGTCTTTCG TTTTTTCTCT
    ATCTGTGGCA AACATGTCAC ACTGAAAATG TTATTATTGA CTGTGGTAGT CAGCATGTAC
    AAGAGCTTCT TCATAATAGT AGGAATGTTT CTGCTGCTTC TCTGCTATGC TTTTGCCGGT
    GTTGTTCTGT TTGGAACAGT GAAATATGGA GAAAACATAA ACAGGCATGC TAACTTTTCC
    ACAGCAGGGA AAGCTATAAC TGTGCTATTC AGAATAGTAA CTGGAGAAGA CTGGAACAAA
    ATAATGCATG ACTGCATGGT ACAGCCTCCT TTTTGCACTC CAGATAACAG CAAATATTGG
    GAAACAGATT GTGGAAATTA TGCAGGGGCA CTTATGTACT TCTGTTCATT TTATGTCATC
    ATTGCTTACA TCATGTTAAA TCTACTGGTT GCCATAATTG TGGAAAACTT TTCCCTCTTC
    TACTCTACTG AAGAAGACCA GCTCCTAAGC CCTAATGATC TGCGCCATTA CCAAATAATA
    TGGAATATGG TTGATGATAA AAGAGAGGGG GTTATTCCAG CTTTTCGTGT GAAGTTCCTT
    CTGAGACTGC TACGCGGGAG GCTGGAGGTT GACTTAGATA AGGACAAGCT TTTGTTCAAA
    CACATGTGCT ATGAAATGGA GAGACTGCAC AGCGGGGGAG ATGTCACTTT CCATGATGTA
    CTTAGCATGC TTTCCTACAG ATCAGTGGAT ATCAGAAAGA GCCTTCAGCT GGAGGAGTTG
    CTAGCCAGAG AACAGCTAGA ATACACAATA GAAGAAGAAG TAGCTAAACA AACAATACGA
    ATGTGGTTGA AAAAGTGTTT GAAACGCATT CGAGCAAAAC AACAGCAATC ATGCAGTATT
    ATACACAGTT TGAGAGAGAG CCAACAGCAG GAACTGAGCA GGTTTCTGAA CCCTCCTAGC
    ATTGAGACCA CACAGCCAAG TGAAGATATG AACCTAAGCA ACCAAGACAA TGCACAGCCA
    GAGACAAGTA GCCAACAACA GCTTCTTAGC CCAACGCTTT CTGACAGAGG AGGTTATCGT
    CAGGATTCAA TTGAAGCTGG TAAACCTCAG AGAAAGTTTG GGCAGTGGCG ATTACCAGCA
    GCACCAAAAC CAATAAGCCA TTCTGTGTCC TCTGTGAACC TACGATTTGG AGGACGGTCA
    ACAATGAAGT CTGTAGTGTG CAAGATGAAC GCCATGTCTG ATGCTGCCTC CTGTGGTTCT
    GAAGTCAAGA AATGGTGGAC AAGACAACTG ACAGTAGAAA GTGATGAGAG TGGTGATGAC
    CTTCTTGATA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35339
    Clone ID Related Accession (Same CDS sequence) XM_012957712.2
    Accession Version XM_012957712.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium leak channel non-selective protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
    GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT
    TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA
    TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT
    CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT
    TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA
    TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC
    ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT
    GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT
    GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT
    GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT
    AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT
    GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT
    GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA
    TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT
    TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT
    ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT
    ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA
    GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG
    AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT
    GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC
    GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC
    TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT
    ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG
    GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA
    CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT
    GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA
    AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT
    CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT
    TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT
    CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC
    GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG
    CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT
    TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT
    CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG
    TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA
    AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA
    TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA
    CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT
    CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA
    GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG
    CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC
    CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA
    AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG
    TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA
    TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA
    GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATCAT GGCAGATGGC
    TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT
    TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA
    ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA
    CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT
    ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG
    AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT
    AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG
    CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA
    GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA
    GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT
    CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG
    AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA
    CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC
    CGCATGTATG ACATAACTCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA
    GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG
    GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA
    GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA
    TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG
    ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG
    AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA
    ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA
    TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG
    CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG
    CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA
    GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA
    CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC
    CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA
    GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG
    GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA
    CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA
    GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC
    ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA
    CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA
    CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA
    GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT
    CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA
    CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT
    GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG
    ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA
    CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012813166.1
    CDS625..5835
    Translation

    Target ORF information:

    RefSeq Version XM_012957712.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium leak channel, non selective (nalcn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957712.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTTGAAAC GAAAGCAAAG CTCAAGAGTG GAAGCACAGC CAGTCACAGA CTTTGGCCCC 
    GATGAATCTC TGTCAGACAA TGCTGATATC CTGTGGATTA ACAAACCATG GGTTCACTCT
    TTACTGCGCA TCTGCGCAAT CATTAGTGTT ATTTCTGTTT GTATGAACAC CCCAAAGACA
    TTTGAACATT ACCCACCCCT CCAGTATGTC ACGTTTGCAC TCGATACCCT ACTTATGTTT
    CTTTACACCG CAGAAATGAT TGCAAAAATG CACATAAGAG GCATTGTAAA GGGTGATACT
    TCCTATGTAA AGGATCGCTG GTGCGTCTTT GATGGATTCA TGGTCTTTTG CCTTTGGGTA
    TCATTGGTGT TGCAGGTATT TGAAATCGCC AAAATCGTTG ACCAAATGTC CCCATGGGGC
    ATGCTGCGGA TCCCACGACC TCTGATAATG ATTCGTGCCT TCCGTATATA CTTCCGCTTT
    GAACTCCCAA GAACGAGGAT AACAAATATC TTGAAGCGTT CAGGAGAGCA AATTTGGAGT
    GTTTCCATAT TTTTATTATT CTTTCTTCTA TTATACGGGA TTCTCGGAGT TCAGATGTTT
    GGAACATTTA CCTTCCATTG TGTTACAAAT GACACAGTAC ATGGAAATCT GACTTGGAAT
    AATTTAGCCA TTCCAGATAC TCACTGCTCC CCTGAAGGTG AAGGATACCA GTGTCCTCGT
    GGTTTTAGGT GCGTGGATCT GAAAGAACTT GGACTGAGCA GGCAAGAGCT TGGCTACAGT
    GGCTTTAATG AAATAGGAAC CAGCATATTT ACGGTTTATG AAGCTGCATC TCAAGAAGGA
    TGGGTTTTTC TTATGTATAG AGCAATAGAC AGCTTCCCCA GATGGCGATC GTACTTCTAT
    TTTATTACCT TAATTTTCTT CCTTGCCTGG CTTGTCAAGA ATGTCTTCAT TGCTGTAATT
    ATTGAAACAT TTGCTGAAAT TCGAGTGCAG TTTCAACAGA TGTGGGGTTC TCGAAGTAGT
    ACTACCTCAA CAGCTACCAC ACAGATGTTT CACGAGGATG CAGCAGGGGG CTGGCAGCTA
    GTGGCAGTTG ATGTGAACAA GCCCCAAGGC AGAGCACCAG CTTGTCTACA GAAAATGATG
    AGATCTTCCA TTTTCCACAT GTTTATTCTG AGCATGGTTG CTGTGGATGT AATTGTTGCT
    GCCAGCAATT ACTACAAAGG AGAGAATTTT AAAAGACAAT ATGATGAGTT TTATCTGGCC
    GAGGTGGCAT TCACCATGCT GTTTGATTTG GAAGCTCTTT TAAAGATTTG GTGCCTGGGC
    TTTACAGGAT ATATCAGTTC ATCACTACAC AAATTTGAGC TTTTGCTTGT AGTTGGAACT
    ACTCTTCATA TTTATCCTGA TCTTTACCAT TCACAGTTTA CATACTTTCA GGTTCTAAGG
    GTGGTGCGAC TGATTAAGAT TTCTCCTGCC TTAGAAGACT TTGTATATAA AATATTTGGA
    CCTGGCAAGA AATTAGGAAG TCTTGTGGTT TTCACCGCTA GCCTATTAAT TGTAATGTCT
    GCCATCAGTT TACAGATGTT CTGCTTTGTG GAAGAGCTGG ACCGTTTTAC AACATTTCCA
    AGGGCATTTA TGTCAATGTT TCAGATTCTG ACACAGGAAG GATGGGTGGA TGTCATGGAT
    CAAACACTTA ATGCCGTTGG GCATATGTGG GCACCAGTTG TTGCAATTTA CTTCATACTT
    TATCATCTTT TTGCTACTTT GATATTGCTT AGTTTGTTTG TGGCTGTTAT CCTGGACAAT
    CTGGAGCTTG ATGAAGACTT GAAAAAGCTT AAACAGTTGA AACAAAGTGA AGCAAATGCC
    GACACCAAAG AAAAATTACC ACTACGCCTA CGTATCTTTG AGAAATTCCC CAACAGACCG
    CAAATGGTGA AGATTTCCAA GCTTCCTTCT GATTTCACAG TTCCAAAAAT AAGGGAGAGT
    TTCATGAAGC AGTTTATTGA CCGCCAGCAA CAAGACACAA GCTGTTTATT AAGAAGGCTT
    CCATCTGCTT CTTCTTCTTC ATGTGATCAT TCCAAACGAT CAGGAGTAGA AGAGAATAAG
    TACATTGACC AAAAGCTTCG TAAATCAGTG TTTAGTATAA GAGCAAGGAA TCTTCTGGAA
    AAGGAAACTG CAGTTAATAA AATCCTCAGA GATTGCACAA GACAGCGCAT GCTGAGTGGA
    TCCTTTGAAG GACAGCCTGC AAAGGAGAGA TCAATACTGA GTGTGCAGCA CCACATAAGA
    CAAGAACGGA GATCACTAAG ACATGGATCA AACAGTCAGA GAATCAGCAG GGGGAAGTCT
    CTGGAAACAC TGACACAAGA CCACTCCAAT ACAGTCAGAT ACAGAAATGC ACAGAGGGAA
    GACAGTGAGA TCAAGATGAT CCAGGAAAAA AAAGAACAAG CAGAAATGAA AAGGAAAGTG
    CAGGAAGAAG AGTTAAGAGA AAACCACCCA TACTTTGATA AGCCACTGTT TATAGTTGGC
    CGTGAACACC GGTTCCGAAA CTTCTGCAGG GTAATAGTTC GGGCACGATT TAATGCCTCA
    AAGACAGATC CTGTTACAGG AGCAGTGAAA AACACCAAAT ATCATCAACT TTATGATTTG
    TTGGGACTAG TGACCTATCT AGACTGGGTT ATGATTGTTG TTACCATATG TTCCTGCATA
    TCAATGATGT TTGAATCTCC TTTTAGAAGA GTAATGCACA CCCCCACGTT ACAGATTGCA
    GAATATGTTT TTGTCATATT CATGAGCATT GAACTGAACT TGAAGATCAT GGCAGATGGC
    TTATTTTTTA CTCCAACGGC TGTTATACGA GACTTTGGGG GTGTTATGGA TATCTTCATT
    TATCTTGTTA GTTTAATCTT CTTATGCTGG ATGCCTAATT ATGTTCCCGC AAAATCTGGA
    ACCCAACTAT TAATGGTGCT GCGCTGCTTG AGACCACTGA GAATATTCAA GCTTGTACCA
    CAGATGAGGA AAGTCGTACG GGAACTTTTC AGTGGGTTTA AAGAAATATT TCTGGTTTCT
    ATTCTTCTGC TGACACTGAT GCTTGTGTTT GCAAGCTTTG GCGTGCAGCT CTTTGCAGGG
    AAACTGGCAA AGTGCAATGA CCCACACATA CTCACCAGGG AAGACTGTCA TGGAATATTT
    AGAATTAATG TCAGTGTTTC GAAAAATTTA AATCTGAAAT TAAAACCAGA GGAAAAGAAG
    CCTGGATTCT GGGTTCCAAG AGTCTGGGCA AATCCCCGTA ATTTTAATTT TGACAATGTA
    GGAAATGCCA TGCTGGCCTT GTTTGAGGTA TTGTCTTTGA AGGGCTGGGT TGAAGTGCGA
    GATGTTATTA TTCATCGAGT TGGACCGATC CATGGAATTT ATATTCATGT CTTTGTGTTT
    CTTGGCTGTA TGATTGGCTT GACTCTCTTT GTTGGTGTGG TTATTGCTAA CTTTAATGAG
    AATAAGGGTA CAGCTTTACT GACTGTTGAT CAAAGGAGAT GGGAAGATCT GAAAAGCAGA
    CTTAAGATAG CCCAACCACT TCATCTCCCC CCACGTCCAG ATAATGATGG TTTCAGAGCC
    CGCATGTATG ACATAACTCA ACACCCTTTT TTCAAGAGGA CTATTGCTGT ACTTGTTTTA
    GCACAGTCTG TGCTTCTGTC AGTAAAGTGG GATGTTGAAG ACCCTGTGAC GGTTCCACTG
    GCAACTATGT CTGTGGTTTT CACCTTCATC TTTGTACTTG AGGTAACCAT GAAGATAACA
    GCTATGTCAC CAGCTGGATT TTGGCAAAGT AGAAGAAATC GCTATGATCT CCTTGTCACA
    TCTCTTGGTG TGATCTGGGT GATACTTCAT TTTGCTTTAC TGAATGAATA CACTTATATG
    ATGGGTGCTT GTATTATCGT CTTTCGTTTT TTCTCTATCT GTGGCAAACA TGTCACACTG
    AAAATGTTAT TATTGACTGT GGTAGTCAGC ATGTACAAGA GCTTCTTCAT AATAGTAGGA
    ATGTTTCTGC TGCTTCTCTG CTATGCTTTT GCCGGTGTTG TTCTGTTTGG AACAGTGAAA
    TATGGAGAAA ACATAAACAG GCATGCTAAC TTTTCCACAG CAGGGAAAGC TATAACTGTG
    CTATTCAGAA TAGTAACTGG AGAAGACTGG AACAAAATAA TGCATGACTG CATGGTACAG
    CCTCCTTTTT GCACTCCAGA TAACAGCAAA TATTGGGAAA CAGATTGTGG AAATTATGCA
    GGGGCACTTA TGTACTTCTG TTCATTTTAT GTCATCATTG CTTACATCAT GTTAAATCTA
    CTGGTTGCCA TAATTGTGGA AAACTTTTCC CTCTTCTACT CTACTGAAGA AGACCAGCTC
    CTAAGCCCTA ATGATCTGCG CCATTACCAA ATAATATGGA ATATGGTTGA TGATAAAAGA
    GAGGGGGTTA TTCCAGCTTT TCGTGTGAAG TTCCTTCTGA GACTGCTACG CGGGAGGCTG
    GAGGTTGACT TAGATAAGGA CAAGCTTTTG TTCAAACACA TGTGCTATGA AATGGAGAGA
    CTGCACAGCG GGGGAGATGT CACTTTCCAT GATGTACTTA GCATGCTTTC CTACAGATCA
    GTGGATATCA GAAAGAGCCT TCAGCTGGAG GAGTTGCTAG CCAGAGAACA GCTAGAATAC
    ACAATAGAAG AAGAAGTAGC TAAACAAACA ATACGAATGT GGTTGAAAAA GTGTTTGAAA
    CGCATTCGAG CAAAACAACA GCAATCATGC AGTATTATAC ACAGTTTGAG AGAGAGCCAA
    CAGCAGGAAC TGAGCAGGTT TCTGAACCCT CCTAGCATTG AGACCACACA GCCAAGTGAA
    GATATGAACC TAAGCAACCA AGACAATGCA CAGCCAGAGA CAAGTAGCCA ACAACAGCTT
    CTTAGCCCAA CGCTTTCTGA CAGAGGAGGT TATCGTCAGG ATTCAATTGA AGCTGGTAAA
    CCTCAGAGAA AGTTTGGGCA GTGGCGATTA CCAGCAGCAC CAAAACCAAT AAGCCATTCT
    GTGTCCTCTG TGAACCTACG ATTTGGAGGA CGGTCAACAA TGAAGTCTGT AGTGTGCAAG
    ATGAACGCCA TGTCTGATGC TGCCTCCTGT GGTTCTGAAG TCAAGAAATG GTGGACAAGA
    CAACTGACAG TAGAAAGTGA TGAGAGTGGT GATGACCTTC TTGATATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.