git1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	git1
Entrez Gene ID	100492231
Full Name	G protein-coupled receptor kinase interacting ArfGAP 1
General protein information	Preferred Names G protein-coupled receptor kinase interacting ArfGAP 1 Names ARF GTPase-activating protein GIT1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091795.1	XP_017947284.1	ARF GTPase-activating protein GIT1
XM_031896668.1	XP_031752528.1	ARF GTPase-activating protein GIT1 isoform X1
XM_031896670.1	XP_031752530.1	ARF GTPase-activating protein GIT1 isoform X3
XM_031896669.1	XP_031752529.1	ARF GTPase-activating protein GIT1 isoform X2

Pathways from BioSystems
Gene Ontology

The following git1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the git1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42917	XM_018091795.1 Latest version!	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa52057	XM_031896668.1 Latest version!	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa52058	XM_031896669.1 Latest version!	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa52059	XM_031896670.1 Latest version!	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

git1 ( XM_018091795.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947284.1 Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), mRNA.

Price & Availability↑

CloneID	OXa42917
Clone ID Related Accession (Same CDS sequence)	XM_018091795.1
Accession Version	XM_018091795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ARF GTPase-activating protein GIT1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGATGAGC TTGGGTGATG TAACAGAACT CATCTTAATT 
TATTGTTTAT ACCTAATGTT CTTGTCCGTG AGTGTGTGTC CATTGCTGTT TAACTTTAGC 
TTTCTCTTCC TTTTCCTTCT TCGCTGCCCT GGATCTAGAA CCCGGCCTCG AGGTGCGCCT 
CAGAGTTTGG ACCTGTCGGA GCTTGCAAAA GCAGCAAAGA AAAAGCTACA AGCACTCAGT 
AACCGGCTGT TTGAGGAGCT CGCTATGGAT GTCTATGATG AAGTTGACAG AAGAGAAAAT 
GATGCAGTTT GGCTGACCAC ACAGAACCAC AGCACTTTGG TCACGGAACG CAGTGCTGTC 
CCCTTTCTTC CAGTTAACCC TGAGTATTCA GCCACAAGGA ATCAGGGGCG CCAAAAGCTG 
GCACGATTCA ATGCACGGGA ATTTGCTACT CTGATCATAG ACATCCTAAG CGAGGCCAAG 
AGGCGACAGC AAGGAAAGAC CCCCGTAATC CCCACAGACA ATCTAGACTA CTCATTCCGG 
AATGCGAATG ACTGCGATGA CCAGCATGAC TATGATAGTG TAGCATCAGA TGAAGACACC 
GACCAGGAGG TGCTCAGGAA CTCCAGAAAC AACAGAGCAA GGAGTATGGA CTCCTCAGAC 
CTGTCAGACG GCCCCATCAC GCTCCAAGAG TACTTAGAAG TTAAAAAAGC ACTTGCGACA 
TCAGACGCCA AAGTGCAGCA ACTGATGAAG GTGAATAACA ATCTGAGTGA GGAGCTGCGT 
CGGCTGCAGA GGGAGATCCA AAAGCTACAA TCAGAGAACA CACAAATTAG ACATCAGACA 
GGTCCTTCAC CTCCACCACC TGCAAACCCT GACCGGACTG AACACCCCGT GTCCGCTGCC 
CACCAACGGG TCCGCCAGGC GTTTTCTATG TATGAGCCGG GCTCTGCACT CAAGCCTTTT 
GGGCAGTCCA CAGATGAACT AAGTAACAGA CTGCAGCCTT TTCACTCTGG GATCCGGAAA 
GGCATTTCTG CCTCTTCTGT GCCCTTTCCC CCCTTGTCTT CCCCTCTGTT CTCTGGACCC 
CCGGACACTG GCCGGAACAT GCCTACAAAA CCGGAGCGGC ATGGCAGCGG CACAGACAGT 
GACTATGATA ATACGCAGAG CACAGAAACC AGCAGCGGGC TCGATGGCAG GCGGTACCTG 
GATGTGTCCC GGGATGAGGA TCCCCACACG GAGGCTGAAG ATGTGGAACT GGAGTTAGAC 
CCTGCCCTGC CCAGCACAGA GGATGTGATC CTTCGAACAG AGCAAGTTAC CAAAAACATC 
CAGGAGCTGC TGCGGGCTGC ACAGGAGTTC AAGCACGACA GCTTTGTCCC TTGCTCGGAA 
AAGATTCACT CAGCAGTAAC GGAGATGGCA TCTCTCTTCC CCAAGAAACC TGCCCTGGAG 
ACAGTGCGAT CATCACTGCG GCTACTGAAC GCTAGCGCCT ACCGACTGCA AAGTGAGTGC 
CGCAAAACCA TCCCGCCAGA ACCTGGGGCC TCTGTGGACT TCCAACTTCT GACACAGCAG 
GTCATCCAGT GCGCTTATGA CATCGCAAAA GCTGCCAAAC AGCTGGTCAC CATCACCACC 
CGCGAGAAGA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947284.1
CDS	418..2655
Translation	MSRKVQRGEVCADCSAPDPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSTWPSSLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKLHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPDKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYGRMSLGDVTELILIYCLYLMFLSVSVCPLLFNFSFLFLFLLRCPGSRTRPRGAPQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKTPVIPTDNLDYSFRNANDCDDQHDYDSVASDEDTDQEVLRNSRNNRARSMDSSDLSDGPITLQEYLEVKKALATSDAKVQQLMKVNNNLSEELRRLQREIQKLQSENTQIRHQTGPSPPPPANPDRTEHPVSAAHQRVRQAFSMYEPGSALKPFGQSTDELSNRLQPFHSGIRKGISASSVPFPPLSSPLFSGPPDTGRNMPTKPERHGSGTDSDYDNTQSTETSSGLDGRRYLDVSRDEDPHTEAEDVELELDPALPSTEDVILRTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTIPPEPGASVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_018091795.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091795.1

ORF Insert Sequence:

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGATGAGC TTGGGTGATG TAACAGAACT CATCTTAATT 
TATTGTTTAT ACCTAATGTT CTTGTCCGTG AGTGTGTGTC CATTGCTGTT TAACTTTAGC 
TTTCTCTTCC TTTTCCTTCT TCGCTGCCCT GGATCTAGAA CCCGGCCTCG AGGTGCGCCT 
CAGAGTTTGG ACCTGTCGGA GCTTGCAAAA GCAGCAAAGA AAAAGCTACA AGCACTCAGT 
AACCGGCTGT TTGAGGAGCT CGCTATGGAT GTCTATGATG AAGTTGACAG AAGAGAAAAT 
GATGCAGTTT GGCTGACCAC ACAGAACCAC AGCACTTTGG TCACGGAACG CAGTGCTGTC 
CCCTTTCTTC CAGTTAACCC TGAGTATTCA GCCACAAGGA ATCAGGGGCG CCAAAAGCTG 
GCACGATTCA ATGCACGGGA ATTTGCTACT CTGATCATAG ACATCCTAAG CGAGGCCAAG 
AGGCGACAGC AAGGAAAGAC CCCCGTAATC CCCACAGACA ATCTAGACTA CTCATTCCGG 
AATGCGAATG ACTGCGATGA CCAGCATGAC TATGATAGTG TAGCATCAGA TGAAGACACC 
GACCAGGAGG TGCTCAGGAA CTCCAGAAAC AACAGAGCAA GGAGTATGGA CTCCTCAGAC 
CTGTCAGACG GCCCCATCAC GCTCCAAGAG TACTTAGAAG TTAAAAAAGC ACTTGCGACA 
TCAGACGCCA AAGTGCAGCA ACTGATGAAG GTGAATAACA ATCTGAGTGA GGAGCTGCGT 
CGGCTGCAGA GGGAGATCCA AAAGCTACAA TCAGAGAACA CACAAATTAG ACATCAGACA 
GGTCCTTCAC CTCCACCACC TGCAAACCCT GACCGGACTG AACACCCCGT GTCCGCTGCC 
CACCAACGGG TCCGCCAGGC GTTTTCTATG TATGAGCCGG GCTCTGCACT CAAGCCTTTT 
GGGCAGTCCA CAGATGAACT AAGTAACAGA CTGCAGCCTT TTCACTCTGG GATCCGGAAA 
GGCATTTCTG CCTCTTCTGT GCCCTTTCCC CCCTTGTCTT CCCCTCTGTT CTCTGGACCC 
CCGGACACTG GCCGGAACAT GCCTACAAAA CCGGAGCGGC ATGGCAGCGG CACAGACAGT 
GACTATGATA ATACGCAGAG CACAGAAACC AGCAGCGGGC TCGATGGCAG GCGGTACCTG 
GATGTGTCCC GGGATGAGGA TCCCCACACG GAGGCTGAAG ATGTGGAACT GGAGTTAGAC 
CCTGCCCTGC CCAGCACAGA GGATGTGATC CTTCGAACAG AGCAAGTTAC CAAAAACATC 
CAGGAGCTGC TGCGGGCTGC ACAGGAGTTC AAGCACGACA GCTTTGTCCC TTGCTCGGAA 
AAGATTCACT CAGCAGTAAC GGAGATGGCA TCTCTCTTCC CCAAGAAACC TGCCCTGGAG 
ACAGTGCGAT CATCACTGCG GCTACTGAAC GCTAGCGCCT ACCGACTGCA AAGTGAGTGC 
CGCAAAACCA TCCCGCCAGA ACCTGGGGCC TCTGTGGACT TCCAACTTCT GACACAGCAG 
GTCATCCAGT GCGCTTATGA CATCGCAAAA GCTGCCAAAC AGCTGGTCAC CATCACCACC 
CGCGAGAAGA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_031896668.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752528.1 Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52057
Clone ID Related Accession (Same CDS sequence)	XM_031896668.1
Accession Version	XM_031896668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GAACCCGGCC TCGAGGTGCG 
CCTCAGAGTT TGGACCTGTC GGAGCTTGCA AAAGCAGCAA AGAAAAAGCT ACAAGCACTC 
AGTAACCGGC TGTTTGAGGA GCTCGCTATG GATGTCTATG ATGAAGTTGA CAGAAGAGAA 
AATGATGCAG TTTGGCTGAC CACACAGAAC CACAGCACTT TGGTCACGGA ACGCAGTGCT 
GTCCCCTTTC TTCCAGTTAA CCCTGAGTAT TCAGCCACAA GGAATCAGGG GCGCCAAAAG 
CTGGCACGAT TCAATGCACG GGAATTTGCT ACTCTGATCA TAGACATCCT AAGCGAGGCC 
AAGAGGCGAC AGCAAGGAAA GACCCCCGTA ATCCCCACAG ACAATCTAGA CTACTCATTC 
CGGAATGCGA ATGACTGCGA TGACCAGCAT GACTATGATA GTGTAGCATC AGATGAAGAC 
ACCGACCAGG AGGTGCTCAG GAACTCCAGA AACAACAGAG CAAGGAGTAT GGACTCCTCA 
GACCTGTCAG ACGGCCCCAT CACGCTCCAA GAGTACTTAG AAGTTAAAAA AGCACTTGCG 
ACATCAGACG CCAAAGTGCA GCAACTGATG AAGGTGAATA ACAATCTGAG TGAGGAGCTG 
CGTCGGCTGC AGAGGGAGAT CCAAAAGCTA CAATCAGAGA ACACACAAAT TAGACATCAG 
ACAGGTCCTT CACCTCCACC ACCTGCAAAC CCTGACCGGA CTGAACACCC CGTGTCCGCT 
GCCCACCAAC GGGTCCGCCA GGCGTTTTCT ATGTATGAGC CGGGCTCTGC ACTCAAGCCT 
TTTGGGCAGT CCACAGATGA ACTAAGTAAC AGACTGCAGC CTTTTCACTC TGGGATCCGG 
AAAGGCATTT CTGCCTCTTC TGTGCCCTTT CCCCCCTTGT CTTCCCCTCT GTTCTCTGGA 
CCCCCGGACA CTGGCCGGAA CATGCCTACA AAACCGGAGC GGCATGGCAG CGGCACAGAC 
AGTGACTATG ATAATACGCA GAGCACAGAA ACCAGCAGCG GGCTCGATGG CAGGCGGTAC 
CTGGATGTGT CCCGGGATGA GGATCCCCAC ACGGAGGCTG AAGATGTGGA ACTGGAGTTA 
GACCCTGCCC TGCCCAGCAC AGAGGATGTG ATCCTTCGAA CAGAGCAAGT TACCAAAAAC 
ATCCAGGAGC TGCTGCGGGC TGCACAGGAG TTCAAGCACG ACAGCTTTGT CCCTTGCTCG 
GAAAAGATTC ACTCAGCAGT AACGGAGATG GCATCTCTCT TCCCCAAGAA ACCTGCCCTG 
GAGACAGTGC GATCATCACT GCGGCTACTG AACGCTAGCG CCTACCGACT GCAAAGTGAG 
TGCCGCAAAA CCATCCCGCC AGAACCTGGG GCCTCTGTGG ACTTCCAACT TCTGACACAG 
CAGGTCATCC AGTGCGCTTA TGACATCGCA AAAGCTGCCA AACAGCTGGT CACCATCACC 
ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752528.1
CDS	344..2584
Translation	MSRKVQRGEVCADCSAPDPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSTWPSSLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKLHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPDKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYGRQAGHHDLADRLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRTRPRGAPQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKTPVIPTDNLDYSFRNANDCDDQHDYDSVASDEDTDQEVLRNSRNNRARSMDSSDLSDGPITLQEYLEVKKALATSDAKVQQLMKVNNNLSEELRRLQREIQKLQSENTQIRHQTGPSPPPPANPDRTEHPVSAAHQRVRQAFSMYEPGSALKPFGQSTDELSNRLQPFHSGIRKGISASSVPFPPLSSPLFSGPPDTGRNMPTKPERHGSGTDSDYDNTQSTETSSGLDGRRYLDVSRDEDPHTEAEDVELELDPALPSTEDVILRTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTIPPEPGASVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_031896668.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896668.1

ORF Insert Sequence:

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GAACCCGGCC TCGAGGTGCG 
CCTCAGAGTT TGGACCTGTC GGAGCTTGCA AAAGCAGCAA AGAAAAAGCT ACAAGCACTC 
AGTAACCGGC TGTTTGAGGA GCTCGCTATG GATGTCTATG ATGAAGTTGA CAGAAGAGAA 
AATGATGCAG TTTGGCTGAC CACACAGAAC CACAGCACTT TGGTCACGGA ACGCAGTGCT 
GTCCCCTTTC TTCCAGTTAA CCCTGAGTAT TCAGCCACAA GGAATCAGGG GCGCCAAAAG 
CTGGCACGAT TCAATGCACG GGAATTTGCT ACTCTGATCA TAGACATCCT AAGCGAGGCC 
AAGAGGCGAC AGCAAGGAAA GACCCCCGTA ATCCCCACAG ACAATCTAGA CTACTCATTC 
CGGAATGCGA ATGACTGCGA TGACCAGCAT GACTATGATA GTGTAGCATC AGATGAAGAC 
ACCGACCAGG AGGTGCTCAG GAACTCCAGA AACAACAGAG CAAGGAGTAT GGACTCCTCA 
GACCTGTCAG ACGGCCCCAT CACGCTCCAA GAGTACTTAG AAGTTAAAAA AGCACTTGCG 
ACATCAGACG CCAAAGTGCA GCAACTGATG AAGGTGAATA ACAATCTGAG TGAGGAGCTG 
CGTCGGCTGC AGAGGGAGAT CCAAAAGCTA CAATCAGAGA ACACACAAAT TAGACATCAG 
ACAGGTCCTT CACCTCCACC ACCTGCAAAC CCTGACCGGA CTGAACACCC CGTGTCCGCT 
GCCCACCAAC GGGTCCGCCA GGCGTTTTCT ATGTATGAGC CGGGCTCTGC ACTCAAGCCT 
TTTGGGCAGT CCACAGATGA ACTAAGTAAC AGACTGCAGC CTTTTCACTC TGGGATCCGG 
AAAGGCATTT CTGCCTCTTC TGTGCCCTTT CCCCCCTTGT CTTCCCCTCT GTTCTCTGGA 
CCCCCGGACA CTGGCCGGAA CATGCCTACA AAACCGGAGC GGCATGGCAG CGGCACAGAC 
AGTGACTATG ATAATACGCA GAGCACAGAA ACCAGCAGCG GGCTCGATGG CAGGCGGTAC 
CTGGATGTGT CCCGGGATGA GGATCCCCAC ACGGAGGCTG AAGATGTGGA ACTGGAGTTA 
GACCCTGCCC TGCCCAGCAC AGAGGATGTG ATCCTTCGAA CAGAGCAAGT TACCAAAAAC 
ATCCAGGAGC TGCTGCGGGC TGCACAGGAG TTCAAGCACG ACAGCTTTGT CCCTTGCTCG 
GAAAAGATTC ACTCAGCAGT AACGGAGATG GCATCTCTCT TCCCCAAGAA ACCTGCCCTG 
GAGACAGTGC GATCATCACT GCGGCTACTG AACGCTAGCG CCTACCGACT GCAAAGTGAG 
TGCCGCAAAA CCATCCCGCC AGAACCTGGG GCCTCTGTGG ACTTCCAACT TCTGACACAG 
CAGGTCATCC AGTGCGCTTA TGACATCGCA AAAGCTGCCA AACAGCTGGT CACCATCACC 
ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_031896669.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752529.1 Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52058
Clone ID Related Accession (Same CDS sequence)	XM_031896669.1
Accession Version	XM_031896669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GTTTGGACCT GTCGGAGCTT 
GCAAAAGCAG CAAAGAAAAA GCTACAAGCA CTCAGTAACC GGCTGTTTGA GGAGCTCGCT 
ATGGATGTCT ATGATGAAGT TGACAGAAGA GAAAATGATG CAGTTTGGCT GACCACACAG 
AACCACAGCA CTTTGGTCAC GGAACGCAGT GCTGTCCCCT TTCTTCCAGT TAACCCTGAG 
TATTCAGCCA CAAGGAATCA GGGGCGCCAA AAGCTGGCAC GATTCAATGC ACGGGAATTT 
GCTACTCTGA TCATAGACAT CCTAAGCGAG GCCAAGAGGC GACAGCAAGG AAAGACCCCC 
GTAATCCCCA CAGACAATCT AGACTACTCA TTCCGGAATG CGAATGACTG CGATGACCAG 
CATGACTATG ATAGTGTAGC ATCAGATGAA GACACCGACC AGGAGGTGCT CAGGAACTCC 
AGAAACAACA GAGCAAGGAG TATGGACTCC TCAGACCTGT CAGACGGCCC CATCACGCTC 
CAAGAGTACT TAGAAGTTAA AAAAGCACTT GCGACATCAG ACGCCAAAGT GCAGCAACTG 
ATGAAGGTGA ATAACAATCT GAGTGAGGAG CTGCGTCGGC TGCAGAGGGA GATCCAAAAG 
CTACAATCAG AGAACACACA AATTAGACAT CAGACAGGTC CTTCACCTCC ACCACCTGCA 
AACCCTGACC GGACTGAACA CCCCGTGTCC GCTGCCCACC AACGGGTCCG CCAGGCGTTT 
TCTATGTATG AGCCGGGCTC TGCACTCAAG CCTTTTGGGC AGTCCACAGA TGAACTAAGT 
AACAGACTGC AGCCTTTTCA CTCTGGGATC CGGAAAGGCA TTTCTGCCTC TTCTGTGCCC 
TTTCCCCCCT TGTCTTCCCC TCTGTTCTCT GGACCCCCGG ACACTGGCCG GAACATGCCT 
ACAAAACCGG AGCGGCATGG CAGCGGCACA GACAGTGACT ATGATAATAC GCAGAGCACA 
GAAACCAGCA GCGGGCTCGA TGGCAGGCGG TACCTGGATG TGTCCCGGGA TGAGGATCCC 
CACACGGAGG CTGAAGATGT GGAACTGGAG TTAGACCCTG CCCTGCCCAG CACAGAGGAT 
GTGATCCTTC GAACAGAGCA AGTTACCAAA AACATCCAGG AGCTGCTGCG GGCTGCACAG 
GAGTTCAAGC ACGACAGCTT TGTCCCTTGC TCGGAAAAGA TTCACTCAGC AGTAACGGAG 
ATGGCATCTC TCTTCCCCAA GAAACCTGCC CTGGAGACAG TGCGATCATC ACTGCGGCTA 
CTGAACGCTA GCGCCTACCG ACTGCAAAGT GAGTGCCGCA AAACCATCCC GCCAGAACCT 
GGGGCCTCTG TGGACTTCCA ACTTCTGACA CAGCAGGTCA TCCAGTGCGC TTATGACATC 
GCAAAAGCTG CCAAACAGCT GGTCACCATC ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752529.1
CDS	341..2554
Translation	MSRKVQRGEVCADCSAPDPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSTWPSSLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKLHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPDKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYGRQAGHHDLADRLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKTPVIPTDNLDYSFRNANDCDDQHDYDSVASDEDTDQEVLRNSRNNRARSMDSSDLSDGPITLQEYLEVKKALATSDAKVQQLMKVNNNLSEELRRLQREIQKLQSENTQIRHQTGPSPPPPANPDRTEHPVSAAHQRVRQAFSMYEPGSALKPFGQSTDELSNRLQPFHSGIRKGISASSVPFPPLSSPLFSGPPDTGRNMPTKPERHGSGTDSDYDNTQSTETSSGLDGRRYLDVSRDEDPHTEAEDVELELDPALPSTEDVILRTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTIPPEPGASVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_031896669.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896669.1

ORF Insert Sequence:

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GTTTGGACCT GTCGGAGCTT 
GCAAAAGCAG CAAAGAAAAA GCTACAAGCA CTCAGTAACC GGCTGTTTGA GGAGCTCGCT 
ATGGATGTCT ATGATGAAGT TGACAGAAGA GAAAATGATG CAGTTTGGCT GACCACACAG 
AACCACAGCA CTTTGGTCAC GGAACGCAGT GCTGTCCCCT TTCTTCCAGT TAACCCTGAG 
TATTCAGCCA CAAGGAATCA GGGGCGCCAA AAGCTGGCAC GATTCAATGC ACGGGAATTT 
GCTACTCTGA TCATAGACAT CCTAAGCGAG GCCAAGAGGC GACAGCAAGG AAAGACCCCC 
GTAATCCCCA CAGACAATCT AGACTACTCA TTCCGGAATG CGAATGACTG CGATGACCAG 
CATGACTATG ATAGTGTAGC ATCAGATGAA GACACCGACC AGGAGGTGCT CAGGAACTCC 
AGAAACAACA GAGCAAGGAG TATGGACTCC TCAGACCTGT CAGACGGCCC CATCACGCTC 
CAAGAGTACT TAGAAGTTAA AAAAGCACTT GCGACATCAG ACGCCAAAGT GCAGCAACTG 
ATGAAGGTGA ATAACAATCT GAGTGAGGAG CTGCGTCGGC TGCAGAGGGA GATCCAAAAG 
CTACAATCAG AGAACACACA AATTAGACAT CAGACAGGTC CTTCACCTCC ACCACCTGCA 
AACCCTGACC GGACTGAACA CCCCGTGTCC GCTGCCCACC AACGGGTCCG CCAGGCGTTT 
TCTATGTATG AGCCGGGCTC TGCACTCAAG CCTTTTGGGC AGTCCACAGA TGAACTAAGT 
AACAGACTGC AGCCTTTTCA CTCTGGGATC CGGAAAGGCA TTTCTGCCTC TTCTGTGCCC 
TTTCCCCCCT TGTCTTCCCC TCTGTTCTCT GGACCCCCGG ACACTGGCCG GAACATGCCT 
ACAAAACCGG AGCGGCATGG CAGCGGCACA GACAGTGACT ATGATAATAC GCAGAGCACA 
GAAACCAGCA GCGGGCTCGA TGGCAGGCGG TACCTGGATG TGTCCCGGGA TGAGGATCCC 
CACACGGAGG CTGAAGATGT GGAACTGGAG TTAGACCCTG CCCTGCCCAG CACAGAGGAT 
GTGATCCTTC GAACAGAGCA AGTTACCAAA AACATCCAGG AGCTGCTGCG GGCTGCACAG 
GAGTTCAAGC ACGACAGCTT TGTCCCTTGC TCGGAAAAGA TTCACTCAGC AGTAACGGAG 
ATGGCATCTC TCTTCCCCAA GAAACCTGCC CTGGAGACAG TGCGATCATC ACTGCGGCTA 
CTGAACGCTA GCGCCTACCG ACTGCAAAGT GAGTGCCGCA AAACCATCCC GCCAGAACCT 
GGGGCCTCTG TGGACTTCCA ACTTCTGACA CAGCAGGTCA TCCAGTGCGC TTATGACATC 
GCAAAAGCTG CCAAACAGCT GGTCACCATC ACCACCCGCG AGAAGAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_031896670.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752530.1 Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

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CloneID	OXa52059
Clone ID Related Accession (Same CDS sequence)	XM_031896670.1
Accession Version	XM_031896670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GAACCCGGCC TCGAGGTGCG 
CCTCAGAGTT TGGACCTGTC GGAGCTTGCA AAAGCAGCAA AGAAAAAGCT ACAAGCACTC 
AGTAACCGGC TGTTTGAGGA GCTCGCTATG GATGTCTATG ATGAAGTTGA CAGAAGAGAA 
AATGATGCAG TTTGGCTGAC CACACAGAAC CACAGCACTT TGGTCACGGA ACGCAGTGCT 
GTCCCCTTTC TTCCAGTTAA CCCTGAGTAT TCAGCCACAA GGAATCAGGG GCGCCAAAAG 
CTGGCACGAT TCAATGCACG GGAATTTGCT ACTCTGATCA TAGACATCCT AAGCGAGGCC 
AAGAGGCGAC AGCAAGGAAA GACCCCCGTA ATCCCCACAG ACAATCTAGA CTACTCATTC 
CGGAATGCGA ATGACTGCGA TGACCAGCAT GACTATGATA GTGTAGCATC AGATGAAGAC 
ACCGACCAGG AGGTGCTCAG GAACTCCAGA AACAACAGAG CAAGGAGTAT GGACTCCTCA 
GACCTGTCAG ACGGCCCCAT CACGCTCCAA GAGTACTTAG AAGTTAAAAA AGCACTTGCG 
ACATCAGACG CCAAAGTGCA GCAACTGATG AAGGTGAATA ACAATCTGAG TGAGGAGCTG 
CGTCGGCTGC AGAGGGAGAT CCAAAAGCTA CAATCAGAGA ACACACAAAT TAGACATCAG 
ACAGGTCCTT CACCTCCACC ACCTGCAAAC CCTGACCGGA CTGAACACCC CGTGTCCGCT 
GCCCACCAAC GGGTCCGCCA GGCGTTTTCT ATGTATGAGC CGGGCTCTGC ACTCAAGCCT 
TTTGGGCAGT CCACAGATGA ACTAAGTAAC AGACTGCAGC CTTTTCACTC TGGGCCTACA 
AAACCGGAGC GGCATGGCAG CGGCACAGAC AGTGACTATG ATAATACGCA GAGCACAGAA 
ACCAGCAGCG GGCTCGATGG CAGGCGGTAC CTGGATGTGT CCCGGGATGA GGATCCCCAC 
ACGGAGGCTG AAGATGTGGA ACTGGAGTTA GACCCTGCCC TGCCCAGCAC AGAGGATGTG 
ATCCTTCGAA CAGAGCAAGT TACCAAAAAC ATCCAGGAGC TGCTGCGGGC TGCACAGGAG 
TTCAAGCACG ACAGCTTTGT CCCTTGCTCG GAAAAGATTC ACTCAGCAGT AACGGAGATG 
GCATCTCTCT TCCCCAAGAA ACCTGCCCTG GAGACAGTGC GATCATCACT GCGGCTACTG 
AACGCTAGCG CCTACCGACT GCAAAGTGAG TGCCGCAAAA CCATCCCGCC AGAACCTGGG 
GCCTCTGTGG ACTTCCAACT TCTGACACAG CAGGTCATCC AGTGCGCTTA TGACATCGCA 
AAAGCTGCCA AACAGCTGGT CACCATCACC ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752530.1
CDS	345..2495
Translation	MSRKVQRGEVCADCSAPDPGWASINRGVLICDECCSVHRSLGRHISIVKHLRHSTWPSSLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKLHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPDKGTTPLHVAAKAGQILQAELLVVYGADPGAPDVNGRTPIDYGRQAGHHDLADRLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRTRPRGAPQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKTPVIPTDNLDYSFRNANDCDDQHDYDSVASDEDTDQEVLRNSRNNRARSMDSSDLSDGPITLQEYLEVKKALATSDAKVQQLMKVNNNLSEELRRLQREIQKLQSENTQIRHQTGPSPPPPANPDRTEHPVSAAHQRVRQAFSMYEPGSALKPFGQSTDELSNRLQPFHSGPTKPERHGSGTDSDYDNTQSTETSSGLDGRRYLDVSRDEDPHTEAEDVELELDPALPSTEDVILRTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHSAVTEMASLFPKKPALETVRSSLRLLNASAYRLQSECRKTIPPEPGASVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_031896670.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896670.1

ORF Insert Sequence:

ATGTCTCGGA AGGTGCAGAG AGGCGAGGTG TGTGCAGACT GCAGCGCTCC AGATCCTGGG 
TGGGCCTCTA TAAATCGTGG CGTTCTGATC TGTGATGAGT GTTGTAGTGT GCACCGAAGC 
TTGGGGAGAC ATATCTCCAT TGTGAAACAT CTCCGGCACA GTACATGGCC ATCGTCTCTC 
TTGCAGATGG TGCACACTTT GGCCAGTAAT GGTGCCAACT CCATTTGGGA GCACTCACTT 
CTGGATCCAG CCCAGGTACA GAGCGGTCGA CGTAAAGCCA ACCCCCAGGA CAAATTACAC 
CCCACAAAGT CCGAGTTCAT TCGTGCCAAG TATCAGATGC TAGCGTTTGT ACATAAGCTA 
CCATGTCGGG ATGACGATGG CGTGACGGCT AAAGATCTGA GCAAGCAACT TCACTCCAGC 
GTGAGGACTG GGAACCTGGA AACCTGCTTG CGGCTGCTAT CTCTCGGGGC TCAGGCCAAT 
TTCTTCCACC CGGACAAAGG AACCACCCCA TTGCATGTTG CAGCCAAGGC TGGACAAATC 
CTTCAGGCAG AGCTGTTAGT GGTCTATGGA GCAGATCCTG GGGCACCGGA TGTGAATGGC 
AGGACACCCA TTGATTATGG CAGGCAGGCT GGTCATCATG ATCTGGCAGA CCGATTGGTG 
GAGTGTCAGT ATGAACTGAC GGATAGGTTG GCCTTTTACC TTTGTGGACG CAAACCAGAT 
CATAAAAATG GTCATTACAT TATCCCCCAG ATGGCTGACA GAACCCGGCC TCGAGGTGCG 
CCTCAGAGTT TGGACCTGTC GGAGCTTGCA AAAGCAGCAA AGAAAAAGCT ACAAGCACTC 
AGTAACCGGC TGTTTGAGGA GCTCGCTATG GATGTCTATG ATGAAGTTGA CAGAAGAGAA 
AATGATGCAG TTTGGCTGAC CACACAGAAC CACAGCACTT TGGTCACGGA ACGCAGTGCT 
GTCCCCTTTC TTCCAGTTAA CCCTGAGTAT TCAGCCACAA GGAATCAGGG GCGCCAAAAG 
CTGGCACGAT TCAATGCACG GGAATTTGCT ACTCTGATCA TAGACATCCT AAGCGAGGCC 
AAGAGGCGAC AGCAAGGAAA GACCCCCGTA ATCCCCACAG ACAATCTAGA CTACTCATTC 
CGGAATGCGA ATGACTGCGA TGACCAGCAT GACTATGATA GTGTAGCATC AGATGAAGAC 
ACCGACCAGG AGGTGCTCAG GAACTCCAGA AACAACAGAG CAAGGAGTAT GGACTCCTCA 
GACCTGTCAG ACGGCCCCAT CACGCTCCAA GAGTACTTAG AAGTTAAAAA AGCACTTGCG 
ACATCAGACG CCAAAGTGCA GCAACTGATG AAGGTGAATA ACAATCTGAG TGAGGAGCTG 
CGTCGGCTGC AGAGGGAGAT CCAAAAGCTA CAATCAGAGA ACACACAAAT TAGACATCAG 
ACAGGTCCTT CACCTCCACC ACCTGCAAAC CCTGACCGGA CTGAACACCC CGTGTCCGCT 
GCCCACCAAC GGGTCCGCCA GGCGTTTTCT ATGTATGAGC CGGGCTCTGC ACTCAAGCCT 
TTTGGGCAGT CCACAGATGA ACTAAGTAAC AGACTGCAGC CTTTTCACTC TGGGCCTACA 
AAACCGGAGC GGCATGGCAG CGGCACAGAC AGTGACTATG ATAATACGCA GAGCACAGAA 
ACCAGCAGCG GGCTCGATGG CAGGCGGTAC CTGGATGTGT CCCGGGATGA GGATCCCCAC 
ACGGAGGCTG AAGATGTGGA ACTGGAGTTA GACCCTGCCC TGCCCAGCAC AGAGGATGTG 
ATCCTTCGAA CAGAGCAAGT TACCAAAAAC ATCCAGGAGC TGCTGCGGGC TGCACAGGAG 
TTCAAGCACG ACAGCTTTGT CCCTTGCTCG GAAAAGATTC ACTCAGCAGT AACGGAGATG 
GCATCTCTCT TCCCCAAGAA ACCTGCCCTG GAGACAGTGC GATCATCACT GCGGCTACTG 
AACGCTAGCG CCTACCGACT GCAAAGTGAG TGCCGCAAAA CCATCCCGCC AGAACCTGGG 
GCCTCTGTGG ACTTCCAACT TCTGACACAG CAGGTCATCC AGTGCGCTTA TGACATCGCA 
AAAGCTGCCA AACAGCTGGT CACCATCACC ACCCGCGAGA AGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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git1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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