arhgef28 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following arhgef28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa35296 XM_012952889.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa42210 XM_018090771.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa49100 XM_031893732.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa04015 XM_002934839.5
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa04016 XM_004910823.4
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35295 XM_012952887.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OXa04016 XM_004910823.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa04015 XM_002934839.4 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa35295 XM_012952887.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa35296 XM_012952889.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa35296
    Clone ID Related Accession (Same CDS sequence) XM_012952889.2 , XM_012952889.3
    Accession Version XM_012952889.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA
    GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC
    CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT
    CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC
    AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG
    AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG
    AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA
    GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG
    AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA
    CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC
    ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT
    GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA
    GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA
    GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA
    TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA
    CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT
    ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG
    GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC
    CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA
    ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC
    TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG
    AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG
    GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG
    GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC
    CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA
    GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG
    AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG
    GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA
    ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC
    CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG
    CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC
    AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT
    CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC
    AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT
    AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT
    GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG
    GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA
    CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT
    TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA
    AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT
    CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA
    GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT
    ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT
    AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC
    CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC
    TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG
    CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT
    GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT
    GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC
    CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA
    GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA
    ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT
    GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC
    TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT
    AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT
    GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA
    AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808343.1
    CDS181..4356
    Translation

    Target ORF information:

    RefSeq Version XM_012952889.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952889.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA
    GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC
    CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT
    CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC
    AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG
    AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG
    AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA
    GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG
    AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA
    CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC
    ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT
    GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA
    GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA
    GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA
    TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA
    CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT
    ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG
    GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC
    CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA
    ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC
    TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG
    AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG
    GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG
    GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC
    CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA
    GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG
    AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG
    GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA
    ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC
    CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG
    CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC
    AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT
    CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC
    AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT
    AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT
    GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG
    GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA
    CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT
    TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA
    AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT
    CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA
    GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT
    ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT
    AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC
    CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC
    TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG
    CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT
    GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT
    GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC
    CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA
    GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA
    ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT
    GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC
    TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT
    AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT
    GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA
    AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42210
    Clone ID Related Accession (Same CDS sequence) XM_018090771.1
    Accession Version XM_018090771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT AAGTAGCCCT
    GTGTTCCTAA CATTTTGTGG CAAGTTAAAG CTGCTTGCTG TGCACTCAGT TAGCCACAAA
    CATATGCTTT ATATTTGTAT GTCTGCATGC ACCGACTCTG CCAGCTCACT TGTCATAAAG
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946260.1
    CDS1083..5288
    Translation

    Target ORF information:

    RefSeq Version XM_018090771.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT AAGTAGCCCT
    GTGTTCCTAA CATTTTGTGG CAAGTTAAAG CTGCTTGCTG TGCACTCAGT TAGCCACAAA
    CATATGCTTT ATATTTGTAT GTCTGCATGC ACCGACTCTG CCAGCTCACT TGTCATAAAG
    ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49100
    Clone ID Related Accession (Same CDS sequence) XM_031893732.1
    Accession Version XM_031893732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGAACAGCGG GCGTATAGCC TCCCTGATCA ACTGAGAGAA
    AAAAGGATTG AGGAAGAGGA ATGGGATAAA TACGCAACTC CTACAAAACC AGAATCTGAA
    AAGAACAAAG TCAGCCGGAC ATTTAGCTTC TTGCGGAACA GGATGACCAG CACCAGGAAC
    AAAAACAAGG TGAAAAATAA GGATATGAAA GAGAAGGTGA ACAGGCATCA GTTTGTGACG
    GGCACGTTCT CTGGGGTGGT TCCATGTTTG GTGTGTGAGA AGGCACTCCT GGGGAAGGAG
    TCTCTGCAGT GCTCCAGTTG TAATGTGAAT GTACACAAAG GCTGCAAGGA ATCTGCTCCC
    CATTGCACCA AGCGATTTCA GGAACGATAT CACCCAAAGA ACAAGCAGTC GGCAGTTATA
    TCAAATTCAT CATATAAAGA TATCCCACAA CAAGTTCAAC CTACAATGCA TACTTCTACA
    TCTGTGCCTC TGGGTCTCTC CTCGGGCAAG AAAGAGACCA TTCACTCCAC TCCCCTGCAT
    TCTAAAACCA TCCCTGTGTT AAGCTCTGAT AGAAGGTCTG ACCCCTCTCT GGAATCAGAT
    GGGGACACCA GTGGCTGCAG ATCGAGATCT CAGTCGGAAG AGCTGCTGCA AACCTTGGGC
    ACACCTCCAT CCATGGACTG CTTCCCAATT GAAGATGTAG TAGATACAAC GTTATGGAGT
    GACTTCAGTA ATGACATGCA GGAATTCGAA GCCGAGTCAT GGAGTCTTGT GGTTGATCCA
    TTATTCTGTG AAAAGCAAAA GAAAGATGTT ATAAAGAGAC AAGATGTCAT TTTTGAACTG
    ATCCAGACAG AACTCCATCA CGTCCAGACA TTGTTGATTA TGTCTGAGAT ATTCAGAAAA
    GGAATGAAAG AGGAGCTTCA GTTGGACCAC AGCACAGTGG ATAAAATATT CCCTTGTCTG
    GATGAGCTGC TGGAGATCCA CAAGCAGTTC TTCTCCAACC TTAAAGAAAG GAAACAGGAA
    TCTAGGGAAG GAAACGACAA AAACTTTATC ATCAACAGAA TTGGAGATAT CCTCGTGCAA
    CAGTTTTCAG CAGACAGTGC CAATAAAATG AAGAAGATCT ATGGAGAGTT TTGCAGTCAC
    CATATCGAAG CACTGAACTT GTTTAAGGAG CTACAGCAGA ACAAGAAATT CCAGAACTTT
    ATTAAACTAA GGAACCGTAA TCTCCTGGCA CGCCGACGGG GTATCCCGGA ATGCATCCTT
    CTTGTAACTC AGCGCATCAC AAAGTATCCA GTGCTTGTGG AAAGGATATT AAAATACACC
    ACCGAAGGGA CAGAGGAGCA CACAGATCTC TGCAAAGCCC TGGTGTTAAT GAAGGAGCTG
    CTGGCAGCAG TGGATTTGCA GGTCAATAAG TTTGAGAAAG GACAAAAGCT GCTAGAGATT
    CTGAATAAAA TTGAAAATAA AACTTGCACC AAATTAAAGA ATGGCCTAGA ATTCAGAAAG
    CAGGACCTGA CGAGCAAAGA GCGGGCGCTG CTGCATGAGG GAGTGGTGTT TTGGAAAACG
    GCTACCGGGC GCTTTAAAGA TATTCAAGCA CTTCTGTTAA CGGACATCCT GCTGTTTCTA
    CAAGAGAAGG ATCAAAAGTA TATTTTTGCT GCAGTGGACC AGAAACCCCC AGTTATTTGC
    CTTCGGAAAC TGATTGTGCG AGAAGTAGCG AATGAGGAGC GCGGGATGTT TTTAATAAGT
    GCCTCATCCG CTGGGCCTGA AATGTATGAG ATCCACACCA ATTCCAAGGA AGAGCGCAAT
    GGCTGGATGA GGCAGATTCA GGAGGCTGTA CAGAGCTGTC CGCAGGAAGA TGAAGGTAAA
    AGCTGTGAAT CAGATGAAGA TAAACGGGTG GCAGAGGCCA GAGCAGTGAA GGCTCAAAAG
    TATCATGATA CGCTGACCCT CCAAGATCAG AATATCTGTA ATACTTTAGA GGAAAAATTA
    CAGATCTACG CAGAACTTGC AGCCTTAAAC ACAAAGAGTG ACATGCACTT GGAACCTCAC
    CTTTTTATCA AAGCCGATTC AGGGGAAATC CCTCAGGCGG CGGCTCTGCT GGCTGCTGCA
    CTCAAGGAAG CTGAAAATCT CCACTCAACC ATTAAATCAC AAATGGAGGA TCCATCTTGG
    TTACCTGAAT ATGAAAGAAG CACTCCGAGA AGATGTCGTT CTGTTGAAAC ATTAGATGAG
    GAACCGGAGA CATCATATAA CTCCCAGAGC CCAGATGCAA GGGAGAGAAG GAAGCTTTCC
    TATGATGCCA GTATTTCTCA TGTGAAGAGT GACAGGGATC TGGATTTCAC AGAAGAAATT
    GAGGAATGTA TGCAAAGTCC AAGCTATAAT CAGGATGAAG TTCTACAAGT GGTGCATAAC
    CTTACTCGCC TGCTGTACAG CATACAGGCT ACTGTTGCTA TGCAAGATAG CCACATAGAA
    CTGCATAAAG CTTTCCTACA GGATTATGAA TTGGGCAGTA GAGGTTCCCG TGGAAGTATA
    CTTAATGAAC ATGAGAAGTA TCGCAACCTA GAAAAGCACC GCTGCGAGAT GGTCAATTTA
    CATAAACTCC AGCAACAGTT GCATCAAGAA CAACAACGCT GGCTGCGCGA GTGCGACCAA
    AAACAGAGGG AAAACGAGGA GAAGGAGAGC ATGCTCCAGC ATAGGGAGCA AGAGTGTCAG
    AGTCAGGAAC ATCTCTTGCA CCAAAAGAGG GAGGAACTTG ACCAACAGCA GCAAGAATTC
    CAGCAAAACG TGGAGCGGCT ACAGGAGGGT CATAGACTTG TGGAGAGGGA GAAGGAGGAT
    CTGGCTGTGA AACTTAAGCT TTGGAGGCAC TTTAACAACC ATCAGCCAAG GAGTCTATTG
    ACTGAGGGTG TCGGAAGCCG TCGCTCTGTC AGTTTACAAG ACGAAGACTC TGTGTATATT
    AACAGTGCCT TGGCTCAGAT ATCACTCAAC ACCATCACAA CATTTCCAGC CCCAGACTAC
    CCGAATGCAC ATTTGTTTCA CAGCCCCAGT TCACTCCTTG CACAGTCTAC TGAGAAAAAC
    ACAACGGACA CTGCTAATGA GCCTTGGAGC TCTGTGGTCT CTCGCCACCA AAGACTTGAA
    GGATCTTCAC TCATTTCCCG GCACAACAAG GATGCATGTA ACAATGATCT GAATGCAGCT
    CATGTTATTT CCTGTGGTAC GCTCCTGTCT GTGCCTAATG CAACGCAGCC TGTGGAACCA
    CAGACCAAAC CCGCAGAAGA AACCTCACAT CATTTGGAAA ATGGCAAAAT TGAAGAGAAC
    ATAGTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031749592.1
    CDS181..4275
    Translation

    Target ORF information:

    RefSeq Version XM_031893732.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031893732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGAACAGCGG GCGTATAGCC TCCCTGATCA ACTGAGAGAA
    AAAAGGATTG AGGAAGAGGA ATGGGATAAA TACGCAACTC CTACAAAACC AGAATCTGAA
    AAGAACAAAG TCAGCCGGAC ATTTAGCTTC TTGCGGAACA GGATGACCAG CACCAGGAAC
    AAAAACAAGG TGAAAAATAA GGATATGAAA GAGAAGGTGA ACAGGCATCA GTTTGTGACG
    GGCACGTTCT CTGGGGTGGT TCCATGTTTG GTGTGTGAGA AGGCACTCCT GGGGAAGGAG
    TCTCTGCAGT GCTCCAGTTG TAATGTGAAT GTACACAAAG GCTGCAAGGA ATCTGCTCCC
    CATTGCACCA AGCGATTTCA GGAACGATAT CACCCAAAGA ACAAGCAGTC GGCAGTTATA
    TCAAATTCAT CATATAAAGA TATCCCACAA CAAGTTCAAC CTACAATGCA TACTTCTACA
    TCTGTGCCTC TGGGTCTCTC CTCGGGCAAG AAAGAGACCA TTCACTCCAC TCCCCTGCAT
    TCTAAAACCA TCCCTGTGTT AAGCTCTGAT AGAAGGTCTG ACCCCTCTCT GGAATCAGAT
    GGGGACACCA GTGGCTGCAG ATCGAGATCT CAGTCGGAAG AGCTGCTGCA AACCTTGGGC
    ACACCTCCAT CCATGGACTG CTTCCCAATT GAAGATGTAG TAGATACAAC GTTATGGAGT
    GACTTCAGTA ATGACATGCA GGAATTCGAA GCCGAGTCAT GGAGTCTTGT GGTTGATCCA
    TTATTCTGTG AAAAGCAAAA GAAAGATGTT ATAAAGAGAC AAGATGTCAT TTTTGAACTG
    ATCCAGACAG AACTCCATCA CGTCCAGACA TTGTTGATTA TGTCTGAGAT ATTCAGAAAA
    GGAATGAAAG AGGAGCTTCA GTTGGACCAC AGCACAGTGG ATAAAATATT CCCTTGTCTG
    GATGAGCTGC TGGAGATCCA CAAGCAGTTC TTCTCCAACC TTAAAGAAAG GAAACAGGAA
    TCTAGGGAAG GAAACGACAA AAACTTTATC ATCAACAGAA TTGGAGATAT CCTCGTGCAA
    CAGTTTTCAG CAGACAGTGC CAATAAAATG AAGAAGATCT ATGGAGAGTT TTGCAGTCAC
    CATATCGAAG CACTGAACTT GTTTAAGGAG CTACAGCAGA ACAAGAAATT CCAGAACTTT
    ATTAAACTAA GGAACCGTAA TCTCCTGGCA CGCCGACGGG GTATCCCGGA ATGCATCCTT
    CTTGTAACTC AGCGCATCAC AAAGTATCCA GTGCTTGTGG AAAGGATATT AAAATACACC
    ACCGAAGGGA CAGAGGAGCA CACAGATCTC TGCAAAGCCC TGGTGTTAAT GAAGGAGCTG
    CTGGCAGCAG TGGATTTGCA GGTCAATAAG TTTGAGAAAG GACAAAAGCT GCTAGAGATT
    CTGAATAAAA TTGAAAATAA AACTTGCACC AAATTAAAGA ATGGCCTAGA ATTCAGAAAG
    CAGGACCTGA CGAGCAAAGA GCGGGCGCTG CTGCATGAGG GAGTGGTGTT TTGGAAAACG
    GCTACCGGGC GCTTTAAAGA TATTCAAGCA CTTCTGTTAA CGGACATCCT GCTGTTTCTA
    CAAGAGAAGG ATCAAAAGTA TATTTTTGCT GCAGTGGACC AGAAACCCCC AGTTATTTGC
    CTTCGGAAAC TGATTGTGCG AGAAGTAGCG AATGAGGAGC GCGGGATGTT TTTAATAAGT
    GCCTCATCCG CTGGGCCTGA AATGTATGAG ATCCACACCA ATTCCAAGGA AGAGCGCAAT
    GGCTGGATGA GGCAGATTCA GGAGGCTGTA CAGAGCTGTC CGCAGGAAGA TGAAGGTAAA
    AGCTGTGAAT CAGATGAAGA TAAACGGGTG GCAGAGGCCA GAGCAGTGAA GGCTCAAAAG
    TATCATGATA CGCTGACCCT CCAAGATCAG AATATCTGTA ATACTTTAGA GGAAAAATTA
    CAGATCTACG CAGAACTTGC AGCCTTAAAC ACAAAGAGTG ACATGCACTT GGAACCTCAC
    CTTTTTATCA AAGCCGATTC AGGGGAAATC CCTCAGGCGG CGGCTCTGCT GGCTGCTGCA
    CTCAAGGAAG CTGAAAATCT CCACTCAACC ATTAAATCAC AAATGGAGGA TCCATCTTGG
    TTACCTGAAT ATGAAAGAAG CACTCCGAGA AGATGTCGTT CTGTTGAAAC ATTAGATGAG
    GAACCGGAGA CATCATATAA CTCCCAGAGC CCAGATGCAA GGGAGAGAAG GAAGCTTTCC
    TATGATGCCA GTATTTCTCA TGTGAAGAGT GACAGGGATC TGGATTTCAC AGAAGAAATT
    GAGGAATGTA TGCAAAGTCC AAGCTATAAT CAGGATGAAG TTCTACAAGT GGTGCATAAC
    CTTACTCGCC TGCTGTACAG CATACAGGCT ACTGTTGCTA TGCAAGATAG CCACATAGAA
    CTGCATAAAG CTTTCCTACA GGATTATGAA TTGGGCAGTA GAGGTTCCCG TGGAAGTATA
    CTTAATGAAC ATGAGAAGTA TCGCAACCTA GAAAAGCACC GCTGCGAGAT GGTCAATTTA
    CATAAACTCC AGCAACAGTT GCATCAAGAA CAACAACGCT GGCTGCGCGA GTGCGACCAA
    AAACAGAGGG AAAACGAGGA GAAGGAGAGC ATGCTCCAGC ATAGGGAGCA AGAGTGTCAG
    AGTCAGGAAC ATCTCTTGCA CCAAAAGAGG GAGGAACTTG ACCAACAGCA GCAAGAATTC
    CAGCAAAACG TGGAGCGGCT ACAGGAGGGT CATAGACTTG TGGAGAGGGA GAAGGAGGAT
    CTGGCTGTGA AACTTAAGCT TTGGAGGCAC TTTAACAACC ATCAGCCAAG GAGTCTATTG
    ACTGAGGGTG TCGGAAGCCG TCGCTCTGTC AGTTTACAAG ACGAAGACTC TGTGTATATT
    AACAGTGCCT TGGCTCAGAT ATCACTCAAC ACCATCACAA CATTTCCAGC CCCAGACTAC
    CCGAATGCAC ATTTGTTTCA CAGCCCCAGT TCACTCCTTG CACAGTCTAC TGAGAAAAAC
    ACAACGGACA CTGCTAATGA GCCTTGGAGC TCTGTGGTCT CTCGCCACCA AAGACTTGAA
    GGATCTTCAC TCATTTCCCG GCACAACAAG GATGCATGTA ACAATGATCT GAATGCAGCT
    CATGTTATTT CCTGTGGTAC GCTCCTGTCT GTGCCTAATG CAACGCAGCC TGTGGAACCA
    CAGACCAAAC CCGCAGAAGA AACCTCACAT CATTTGGAAA ATGGCAAAAT TGAAGAGAAC
    ATAGTTTACC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04015
    Clone ID Related Accession (Same CDS sequence) XM_002934839.4 , XM_002934839.5
    Accession Version XM_002934839.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934839.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG
    GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC
    CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT
    GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG
    GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG
    TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA
    GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG
    CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG
    AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG
    AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT
    GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC
    CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT
    GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA
    AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG
    AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT
    GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT
    GAAGAGAACA TAGTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934885.2
    CDS1071..6074
    Translation

    Target ORF information:

    RefSeq Version XM_002934839.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934839.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG
    GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC
    CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT
    GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG
    GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG
    TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA
    GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG
    CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG
    AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG
    AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT
    GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC
    CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT
    GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA
    AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG
    AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT
    GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT
    GAAGAGAACA TAGTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04016
    Clone ID Related Accession (Same CDS sequence) XM_004910823.3 , XM_004910823.4
    Accession Version XM_004910823.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA
    TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC
    TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA
    TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT
    GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT
    GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC
    AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT
    TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA
    CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC
    GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC
    AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG
    AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC
    ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA
    TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG
    GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA
    GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC
    AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG
    AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG
    TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT
    ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA
    ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG
    CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA
    GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT
    CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA
    AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA
    CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT
    GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA
    TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA
    GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG
    CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA
    GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG
    CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC
    ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA
    AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC
    AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC
    GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG
    TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA
    GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG
    GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT
    CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG
    TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA
    GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG
    AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA
    CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT
    GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG
    GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA
    GAGAACATAG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910880.1
    CDS1072..6072
    Translation

    Target ORF information:

    RefSeq Version XM_004910823.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910823.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA
    TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC
    TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA
    TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT
    GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT
    GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC
    AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT
    TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA
    CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC
    GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC
    AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG
    AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC
    ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA
    TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG
    GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA
    GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC
    AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG
    AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG
    TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT
    ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA
    ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG
    CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA
    GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT
    CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA
    AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA
    CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT
    GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA
    TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA
    GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG
    CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA
    GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG
    CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC
    ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA
    AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC
    AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC
    GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG
    TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA
    GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG
    GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT
    CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG
    TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA
    GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG
    AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA
    CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT
    GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG
    GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA
    GAGAACATAG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35295
    Clone ID Related Accession (Same CDS sequence) XM_012952887.3 , XM_012952887.2
    Accession Version XM_012952887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG
    GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC
    AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG
    AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT
    GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC
    TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG
    CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA
    TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG
    GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC
    CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT
    GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC
    ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT
    GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA
    AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA
    CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG
    GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG
    GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA
    AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA
    GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA
    CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG
    AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG
    CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA
    GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG
    GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT
    GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG
    AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC
    TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT
    CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG
    ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT
    GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG
    CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA
    GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG
    CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA
    GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA
    GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT
    GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT
    GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA
    TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT
    ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG
    CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG
    CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT
    TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT
    GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG
    CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA
    AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT
    CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG
    GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA
    CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC
    GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG
    GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT
    TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT
    GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC
    ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC
    TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC
    GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808341.1
    CDS1071..5993
    Translation

    Target ORF information:

    RefSeq Version XM_012952887.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG
    GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC
    AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG
    AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT
    GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC
    TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG
    CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA
    TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG
    GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC
    CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT
    GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC
    ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT
    GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA
    AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA
    CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG
    GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG
    GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA
    AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA
    GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA
    CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG
    AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG
    CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA
    GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG
    GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT
    GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG
    AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC
    TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT
    CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG
    ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT
    GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG
    CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA
    GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG
    CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA
    GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA
    GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT
    GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT
    GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA
    TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT
    ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG
    CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG
    CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT
    TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT
    GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG
    CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA
    AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT
    CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG
    GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA
    CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC
    GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG
    GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT
    TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT
    GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC
    ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC
    TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC
    GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04015
    Clone ID Related Accession (Same CDS sequence) XM_002934839.4 , XM_002934839.5
    Accession Version XM_002934839.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934839.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG
    GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC
    CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT
    GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG
    GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG
    TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA
    GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG
    CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG
    AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG
    AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT
    GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC
    CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT
    GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA
    AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG
    AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT
    GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT
    GAAGAGAACA TAGTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934885.2
    CDS1081..6084
    Translation

    Target ORF information:

    RefSeq Version XM_002934839.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934839.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG
    GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA
    ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG
    TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG
    GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT
    TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA
    TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC
    CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG
    AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC
    CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT
    TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC
    CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA
    TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA
    AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG
    AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG
    CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA
    TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT
    TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG
    CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA
    GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT
    TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA
    GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT
    GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG
    GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG
    GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG
    GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG
    GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT
    CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA
    TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG
    AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA
    GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG
    GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC
    CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT
    GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG
    GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG
    TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA
    GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG
    CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG
    AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG
    AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT
    GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC
    CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT
    GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA
    AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG
    AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT
    GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT
    GAAGAGAACA TAGTTTACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04016
    Clone ID Related Accession (Same CDS sequence) XM_004910823.3 , XM_004910823.4
    Accession Version XM_004910823.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA
    TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC
    TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA
    TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT
    GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT
    GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC
    AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT
    TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA
    CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC
    GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC
    AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG
    AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC
    ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA
    TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG
    GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA
    GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC
    AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG
    AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG
    TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT
    ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA
    ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG
    CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA
    GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT
    CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA
    AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA
    CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT
    GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA
    TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA
    GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG
    CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA
    GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG
    CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC
    ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA
    AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC
    AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC
    GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG
    TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA
    GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG
    GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT
    CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG
    TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA
    GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG
    AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA
    CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT
    GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG
    GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA
    GAGAACATAG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910880.1
    CDS1081..6081
    Translation

    Target ORF information:

    RefSeq Version XM_004910823.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910823.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG
    TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA
    CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA
    GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC
    ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG
    TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG
    GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA
    TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG
    GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT
    ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT
    CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA
    TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC
    TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA
    TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT
    GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT
    GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC
    AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT
    TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA
    CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC
    GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC
    AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG
    AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC
    ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA
    TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG
    GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA
    GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC
    AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG
    AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG
    TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT
    ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA
    ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG
    CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA
    GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT
    CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA
    AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA
    CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT
    GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA
    TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA
    GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG
    CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA
    GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG
    CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC
    ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA
    AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC
    AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC
    GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG
    TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA
    GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG
    GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT
    CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG
    TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA
    GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG
    AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA
    CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT
    GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG
    GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA
    GAGAACATAG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35295
    Clone ID Related Accession (Same CDS sequence) XM_012952887.3 , XM_012952887.2
    Accession Version XM_012952887.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG
    GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC
    AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG
    AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT
    GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC
    TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG
    CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA
    TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG
    GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC
    CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT
    GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC
    ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT
    GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA
    AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA
    CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG
    GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG
    GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA
    AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA
    GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA
    CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG
    AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG
    CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA
    GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG
    GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT
    GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG
    AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC
    TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT
    CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG
    ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT
    GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG
    CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA
    GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG
    CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA
    GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA
    GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT
    GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT
    GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA
    TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT
    ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG
    CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG
    CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT
    TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT
    GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG
    CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA
    AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT
    CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG
    GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA
    CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC
    GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG
    GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT
    TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT
    GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC
    ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC
    TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC
    GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808341.1
    CDS1081..6003
    Translation

    Target ORF information:

    RefSeq Version XM_012952887.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
    TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC
    AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT
    CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC
    TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC
    TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC
    ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC
    CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC
    AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT
    CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG
    GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA
    AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC
    TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG
    TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA
    AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA
    CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG
    CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT
    GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC
    AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA
    AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT
    CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA
    CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA
    AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA
    TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT
    GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA
    TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT
    TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC
    ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG
    GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC
    AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG
    AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT
    GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC
    TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG
    CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA
    TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG
    GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC
    CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT
    GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC
    ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT
    GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA
    AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA
    CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG
    GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG
    GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA
    AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA
    GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA
    CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG
    AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG
    CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA
    GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG
    GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT
    GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG
    AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC
    TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT
    CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG
    ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT
    GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG
    CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA
    GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG
    CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA
    GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA
    GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT
    GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT
    GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA
    TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT
    ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG
    CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG
    CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT
    TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT
    GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG
    CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA
    AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT
    CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG
    GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA
    CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC
    GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG
    GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT
    TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT
    GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC
    ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC
    TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC
    GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35296
    Clone ID Related Accession (Same CDS sequence) XM_012952889.2 , XM_012952889.3
    Accession Version XM_012952889.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA
    GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC
    CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT
    CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC
    AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG
    AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG
    AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA
    GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG
    AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA
    CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC
    ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT
    GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA
    GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA
    GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA
    TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA
    CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT
    ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG
    GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC
    CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA
    ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC
    TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG
    AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG
    GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG
    GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC
    CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA
    GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG
    AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG
    GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA
    ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC
    CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG
    CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC
    AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT
    CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC
    AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT
    AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT
    GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG
    GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA
    CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT
    TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA
    AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT
    CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA
    GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT
    ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT
    AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC
    CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC
    TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG
    CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT
    GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT
    GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC
    CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA
    GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA
    ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT
    GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC
    TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT
    AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT
    GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA
    AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808343.1
    CDS289..4464
    Translation

    Target ORF information:

    RefSeq Version XM_012952889.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
    ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG
    TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT
    GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC
    ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG
    ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA
    AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT
    GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT
    GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA
    ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA
    AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA
    GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT
    TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC
    TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA
    GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC
    CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT
    CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC
    AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG
    AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG
    AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA
    GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG
    AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA
    CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC
    ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT
    GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA
    GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA
    GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA
    TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA
    CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT
    ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG
    GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC
    CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA
    ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC
    TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG
    AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG
    GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG
    GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC
    CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA
    GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG
    AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG
    GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA
    ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC
    CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG
    CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC
    AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT
    CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC
    AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT
    AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT
    GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG
    GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA
    CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT
    TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA
    AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT
    CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA
    GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT
    ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT
    AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC
    CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC
    TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG
    CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT
    GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT
    GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC
    CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA
    GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA
    ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT
    GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC
    TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT
    AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT
    GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA
    AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.