cacna1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1a
Entrez Gene ID	100492135
Full Name	calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Synonyms	ca(v)2.1,cav2.1
General protein information	Preferred Names calcium channel, voltage-dependent, P/Q type, alpha 1A subunit Names voltage-dependent P/Q-type calcium channel subunit alpha-1A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031899454.1	XP_031755314.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_031899452.1	XP_031755312.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_031899456.1	XP_031755316.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
XM_018092693.1	XP_017948182.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A
XM_031899451.1	XP_031755311.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_031899455.1	XP_031755315.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
XM_031899453.1	XP_031755313.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_031899457.1	XP_031755317.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1A	NP_001120694.1
Pan troglodytes (chimpanzee)	CACNA1A	XP_003316202.1
Xenopus tropicalis (tropical clawed frog)	cacna1a	XP_004919196.1
	CACNA1A	XP_002801141.1
Danio rerio (zebrafish)	cacna1ab	XP_698014.6
Canis lupus familiaris (dog)	CACNA1A	XP_005632867.1
Bos taurus (cattle)	CACNA1A	NP_001068597.1
Mus musculus (house mouse)	Cacna1a	NP_031604.3
Rattus norvegicus (Norway rat)	Cacna1a	NP_037050.2

Gene Ontology

The following cacna1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa55401	XM_031899451.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55402	XM_031899452.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55403	XM_031899453.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55404	XM_031899454.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55405	XM_031899455.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55406	XM_031899456.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa55407	XM_031899457.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa43582	XM_018092693.1 Latest version!	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1a ( XM_031899451.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755311.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55401
Clone ID Related Accession (Same CDS sequence)	XM_031899451.1
Accession Version	XM_031899451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGGGCAGT AGTTCAGTGG ATGGCAGCCC CGTGCCCTCA 
ACCTCTGGGG CCAGCACACC CCGCAGAGGA AGGCGACAGC TGCCCCAGCT GCCAACAACT 
CCCAGGCCAC GAGTTACTTA TTCTCCAGTG GTCCGCCGGC CTGCCAGCGT TTGCTCTCCC 
CAGCAGCAGT CCTTGTGCAG AATGACCACC CCTATCCAAC GGAGATATCC CCAGACTGAG 
AACCGTCCAA GCAGCAATGA AAGACGGTCC GTTAACAATG ATTCCCCCCG ATCCCATCGT 
CACACAAGAG GACACTGCAC GGAAGTCCCC CGCGCTGGAT GCAGGCACAG CGGCCCTTAT 
CGAGATTCCT CTTTTGAGGA CCCTCATCCC TGCGATCAGC GCTCCTCCAG GACTGCCGAG 
TCTGGGGGGG TACCCCCTCT CTCACCGCGC GGGCGCAGGT TGCCCAATGG TTACTCTCAT 
GGGACCCCGC GATCCACAAA ATCACGTCAT GAACGTTACA GTGAGAGTGA GGAGGATGAC 
TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755311.1
CDS	9..6917
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899451.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899451.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGGGCAGT AGTTCAGTGG ATGGCAGCCC CGTGCCCTCA 
ACCTCTGGGG CCAGCACACC CCGCAGAGGA AGGCGACAGC TGCCCCAGCT GCCAACAACT 
CCCAGGCCAC GAGTTACTTA TTCTCCAGTG GTCCGCCGGC CTGCCAGCGT TTGCTCTCCC 
CAGCAGCAGT CCTTGTGCAG AATGACCACC CCTATCCAAC GGAGATATCC CCAGACTGAG 
AACCGTCCAA GCAGCAATGA AAGACGGTCC GTTAACAATG ATTCCCCCCG ATCCCATCGT 
CACACAAGAG GACACTGCAC GGAAGTCCCC CGCGCTGGAT GCAGGCACAG CGGCCCTTAT 
CGAGATTCCT CTTTTGAGGA CCCTCATCCC TGCGATCAGC GCTCCTCCAG GACTGCCGAG 
TCTGGGGGGG TACCCCCTCT CTCACCGCGC GGGCGCAGGT TGCCCAATGG TTACTCTCAT 
GGGACCCCGC GATCCACAAA ATCACGTCAT GAACGTTACA GTGAGAGTGA GGAGGATGAC 
TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899452.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755312.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X2, mRNA.

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CloneID	OXa55402
Clone ID Related Accession (Same CDS sequence)	XM_031899452.1
Accession Version	XM_031899452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGCGGGC GTATTCATTA TAAGGACATG TACAGTTTGT TACGGGTTAT CTCCCCTCCG 
CTGGGGTTGG GGAAGAAATG CCCCCACAGG GTGGCCTGCA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGGGCAGT AGTTCAGTGG ATGGCAGCCC CGTGCCCTCA 
ACCTCTGGGG CCAGCACACC CCGCAGAGGA AGGCGACAGC TGCCCCAGCT GCCAACAACT 
CCCAGGCCAC GAGTTACTTA TTCTCCAGTG GTCCGCCGGC CTGCCAGCGT TTGCTCTCCC 
CAGCAGCAGT CCTTGTGCAG AATGACCACC CCTATCCAAC GGAGATATCC CCAGACTGAG 
AACCGTCCAA GCAGCAATGA AAGACGGTCC GTTAACAATG ATTCCCCCCG ATCCCATCGT 
CACACAAGAG GACACTGCAC GGAAGTCCCC CGCGCTGGAT GCAGGCACAG CGGCCCTTAT 
CGAGATTCCT CTTTTGAGGA CCCTCATCCC TGCGATCAGC GCTCCTCCAG GACTGCCGAG 
TCTGGGGGGG TACCCCCTCT CTCACCGCGC GGGCGCAGGT TGCCCAATGG TTACTCTCAT 
GGGACCCCGC GATCCACAAA ATCACGTCAT GAACGTTACA GTGAGAGTGA GGAGGATGAC 
TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755312.1
CDS	9..6917
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899452.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899452.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGCGGGC GTATTCATTA TAAGGACATG TACAGTTTGT TACGGGTTAT CTCCCCTCCG 
CTGGGGTTGG GGAAGAAATG CCCCCACAGG GTGGCCTGCA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGGGCAGT AGTTCAGTGG ATGGCAGCCC CGTGCCCTCA 
ACCTCTGGGG CCAGCACACC CCGCAGAGGA AGGCGACAGC TGCCCCAGCT GCCAACAACT 
CCCAGGCCAC GAGTTACTTA TTCTCCAGTG GTCCGCCGGC CTGCCAGCGT TTGCTCTCCC 
CAGCAGCAGT CCTTGTGCAG AATGACCACC CCTATCCAAC GGAGATATCC CCAGACTGAG 
AACCGTCCAA GCAGCAATGA AAGACGGTCC GTTAACAATG ATTCCCCCCG ATCCCATCGT 
CACACAAGAG GACACTGCAC GGAAGTCCCC CGCGCTGGAT GCAGGCACAG CGGCCCTTAT 
CGAGATTCCT CTTTTGAGGA CCCTCATCCC TGCGATCAGC GCTCCTCCAG GACTGCCGAG 
TCTGGGGGGG TACCCCCTCT CTCACCGCGC GGGCGCAGGT TGCCCAATGG TTACTCTCAT 
GGGACCCCGC GATCCACAAA ATCACGTCAT GAACGTTACA GTGAGAGTGA GGAGGATGAC 
TGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899453.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755313.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X3, mRNA.

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CloneID	OXa55403
Clone ID Related Accession (Same CDS sequence)	XM_031899453.1
Accession Version	XM_031899453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAATCT 
GTAGAGATGA GTGACATGGG TCGGGACGGT TACTCAGACA GCGAGCAGTA CCTGGTCATG 
GAGGGACATG GAAGAGCCGC TTCAATGCCA CGGCTTCCAG CTGATAACCA GCAGCGAAGG 
AAAACCCGAA CCAGAGGGAG TAATCTCAGT ACTATTACGG ACGGGAGTCC TATGAAACGT 
TCCGCCTCCA TGCTCGGCCA CTCCCAGTCT AAAGGCATCA GATTGGATGA GTATTCATTA 
GAGAGGGTTA CACCTGAGGA AGGACCTCGC CATGGGCACC AACGCAGGAG GGAGAGGGGA 
CACAGGGTCT CTGACAGATC CCTCACCCGA TATGTGGATC TGGAGTCAGG ATTGGGTACA 
GAGCTCAGCA CTACAACACA GTCATGTGAT CTCCCCACTG AAGAGCGTGG ACGCACTCGA 
GATCGGAAGC ACCACCATCA CCACCACCAC CATCATCATC ACCATCATCA CCACCCCAGC 
ACTGAACGGG AGAGATTAGA GTACGGAAGG CCAAGCTCTC GCTCCCCCAG CACAGGGCGA 
GATTATGCTC CACACAGACA GGGCAGTAGT TCAGTGGATG GCAGCCCCGT GCCCTCAACC 
TCTGGGGCCA GCACACCCCG CAGAGGAAGG CGACAGCTGC CCCAGCTGCC AACAACTCCC 
AGGCCACGAG TTACTTATTC TCCAGTGGTC CGCCGGCCTG CCAGCGTTTG CTCTCCCCAG 
CAGCAGTCCT TGTGCAGAAT GACCACCCCT ATCCAACGGA GATATCCCCA GACTGAGAAC 
CGTCCAAGCA GCAATGAAAG ACGGTCCGTT AACAATGATT CCCCCCGATC CCATCGTCAC 
ACAAGAGGAC ACTGCACGGA AGTCCCCCGC GCTGGATGCA GGCACAGCGG CCCTTATCGA 
GATTCCTCTT TTGAGGACCC TCATCCCTGC GATCAGCGCT CCTCCAGGAC TGCCGAGTCT 
GGGGGGGTAC CCCCTCTCTC ACCGCGCGGG CGCAGGTTGC CCAATGGTTA CTCTCATGGG 
ACCCCGCGAT CCACAAAATC ACGTCATGAA CGTTACAGTG AGAGTGAGGA GGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755313.1
CDS	9..6914
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899453.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899453.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAATCT 
GTAGAGATGA GTGACATGGG TCGGGACGGT TACTCAGACA GCGAGCAGTA CCTGGTCATG 
GAGGGACATG GAAGAGCCGC TTCAATGCCA CGGCTTCCAG CTGATAACCA GCAGCGAAGG 
AAAACCCGAA CCAGAGGGAG TAATCTCAGT ACTATTACGG ACGGGAGTCC TATGAAACGT 
TCCGCCTCCA TGCTCGGCCA CTCCCAGTCT AAAGGCATCA GATTGGATGA GTATTCATTA 
GAGAGGGTTA CACCTGAGGA AGGACCTCGC CATGGGCACC AACGCAGGAG GGAGAGGGGA 
CACAGGGTCT CTGACAGATC CCTCACCCGA TATGTGGATC TGGAGTCAGG ATTGGGTACA 
GAGCTCAGCA CTACAACACA GTCATGTGAT CTCCCCACTG AAGAGCGTGG ACGCACTCGA 
GATCGGAAGC ACCACCATCA CCACCACCAC CATCATCATC ACCATCATCA CCACCCCAGC 
ACTGAACGGG AGAGATTAGA GTACGGAAGG CCAAGCTCTC GCTCCCCCAG CACAGGGCGA 
GATTATGCTC CACACAGACA GGGCAGTAGT TCAGTGGATG GCAGCCCCGT GCCCTCAACC 
TCTGGGGCCA GCACACCCCG CAGAGGAAGG CGACAGCTGC CCCAGCTGCC AACAACTCCC 
AGGCCACGAG TTACTTATTC TCCAGTGGTC CGCCGGCCTG CCAGCGTTTG CTCTCCCCAG 
CAGCAGTCCT TGTGCAGAAT GACCACCCCT ATCCAACGGA GATATCCCCA GACTGAGAAC 
CGTCCAAGCA GCAATGAAAG ACGGTCCGTT AACAATGATT CCCCCCGATC CCATCGTCAC 
ACAAGAGGAC ACTGCACGGA AGTCCCCCGC GCTGGATGCA GGCACAGCGG CCCTTATCGA 
GATTCCTCTT TTGAGGACCC TCATCCCTGC GATCAGCGCT CCTCCAGGAC TGCCGAGTCT 
GGGGGGGTAC CCCCTCTCTC ACCGCGCGGG CGCAGGTTGC CCAATGGTTA CTCTCATGGG 
ACCCCGCGAT CCACAAAATC ACGTCATGAA CGTTACAGTG AGAGTGAGGA GGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899454.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755314.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X4, mRNA.

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CloneID	OXa55404
Clone ID Related Accession (Same CDS sequence)	XM_031899454.1
Accession Version	XM_031899454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCGAAGG 
AAAACCCGAA CCAGAGGGAG TAATCTCAGT ACTATTACGG ACGGGAGTCC TATGAAACGT 
TCCGCCTCCA TGCTCGGCCA CTCCCAGTCT AAAGGCATCA GATTGGATGA GTATTCATTA 
GAGAGGGTTA CACCTGAGGA AGGACCTCGC CATGGGCACC AACGCAGGAG GGAGAGGGGA 
CACAGGGTCT CTGACAGATC CCTCACCCGA TATGTGGATC TGGAGTCAGG ATTGGGTACA 
GAGCTCAGCA CTACAACACA GTCATGTGAT CTCCCCACTG AAGAGCGTGG ACGCACTCGA 
GATCGGAAGC ACCACCATCA CCACCACCAC CATCATCATC ACCATCATCA CCACCCCAGC 
ACTGAACGGG AGAGATTAGA GTACGGAAGG CCAAGCTCTC GCTCCCCCAG CACAGGGCGA 
GATTATGCTC CACACAGACA GGGCAGTAGT TCAGTGGATG GCAGCCCCGT GCCCTCAACC 
TCTGGGGCCA GCACACCCCG CAGAGGAAGG CGACAGCTGC CCCAGCTGCC AACAACTCCC 
AGGCCACGAG TTACTTATTC TCCAGTGGTC CGCCGGCCTG CCAGCGTTTG CTCTCCCCAG 
CAGCAGTCCT TGTGCAGAAT GACCACCCCT ATCCAACGGA GATATCCCCA GACTGAGAAC 
CGTCCAAGCA GCAATGAAAG ACGGTCCGTT AACAATGATT CCCCCCGATC CCATCGTCAC 
ACAAGAGGAC ACTGCACGGA AGTCCCCCGC GCTGGATGCA GGCACAGCGG CCCTTATCGA 
GATTCCTCTT TTGAGGACCC TCATCCCTGC GATCAGCGCT CCTCCAGGAC TGCCGAGTCT 
GGGGGGGTAC CCCCTCTCTC ACCGCGCGGG CGCAGGTTGC CCAATGGTTA CTCTCATGGG 
ACCCCGCGAT CCACAAAATC ACGTCATGAA CGTTACAGTG AGAGTGAGGA GGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755314.1
CDS	9..6914
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899454.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899454.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCGAAGG 
AAAACCCGAA CCAGAGGGAG TAATCTCAGT ACTATTACGG ACGGGAGTCC TATGAAACGT 
TCCGCCTCCA TGCTCGGCCA CTCCCAGTCT AAAGGCATCA GATTGGATGA GTATTCATTA 
GAGAGGGTTA CACCTGAGGA AGGACCTCGC CATGGGCACC AACGCAGGAG GGAGAGGGGA 
CACAGGGTCT CTGACAGATC CCTCACCCGA TATGTGGATC TGGAGTCAGG ATTGGGTACA 
GAGCTCAGCA CTACAACACA GTCATGTGAT CTCCCCACTG AAGAGCGTGG ACGCACTCGA 
GATCGGAAGC ACCACCATCA CCACCACCAC CATCATCATC ACCATCATCA CCACCCCAGC 
ACTGAACGGG AGAGATTAGA GTACGGAAGG CCAAGCTCTC GCTCCCCCAG CACAGGGCGA 
GATTATGCTC CACACAGACA GGGCAGTAGT TCAGTGGATG GCAGCCCCGT GCCCTCAACC 
TCTGGGGCCA GCACACCCCG CAGAGGAAGG CGACAGCTGC CCCAGCTGCC AACAACTCCC 
AGGCCACGAG TTACTTATTC TCCAGTGGTC CGCCGGCCTG CCAGCGTTTG CTCTCCCCAG 
CAGCAGTCCT TGTGCAGAAT GACCACCCCT ATCCAACGGA GATATCCCCA GACTGAGAAC 
CGTCCAAGCA GCAATGAAAG ACGGTCCGTT AACAATGATT CCCCCCGATC CCATCGTCAC 
ACAAGAGGAC ACTGCACGGA AGTCCCCCGC GCTGGATGCA GGCACAGCGG CCCTTATCGA 
GATTCCTCTT TTGAGGACCC TCATCCCTGC GATCAGCGCT CCTCCAGGAC TGCCGAGTCT 
GGGGGGGTAC CCCCTCTCTC ACCGCGCGGG CGCAGGTTGC CCAATGGTTA CTCTCATGGG 
ACCCCGCGAT CCACAAAATC ACGTCATGAA CGTTACAGTG AGAGTGAGGA GGATGACTGG 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899455.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755315.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X5, mRNA.

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CloneID	OXa55405
Clone ID Related Accession (Same CDS sequence)	XM_031899455.1
Accession Version	XM_031899455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCA GGTGGAAGCA AAGGAAAGGA TATAAGTACC 
ATCAAGTCAC TGCGTGTTCT GCGTGTCCTG CGTCCTCTGA AAACAATCAA ACGGCTTCCC 
AAGCTAAAGG CCGTGTTCGA CTGCGTAGTC AACTCTCTGA AGAACGTTCT GAACATTCTC 
ATTGTCTACA TGCTCTTCAT GTTCATCTTC GCTGTTGTAG CCGTCCAGCT TTTCAAGGGG 
AAGTTCTTTT ATTGTACAGA TGAGTCCAAG GAATTCCAGA GAGACTGCAG GGGAGAATAC 
CTGGTCTATG ACAAAAGTGA AGTTAAAGCA CAGAAACGAG AGTGGAAGAA ATGGCCGTTC 
CATTATGACA ATGTGCTATG GGCACTTCTC ACCCTCTTTA CTGTATCTAC AGGGGAAGGT 
TGGCCAGATG TGCTCAGACA TTCTGTGGAT GCCACCTATG AGGATCAGGG TCCCAGGCCT 
GGTTTTCGGA TGGAGATGTC TATATTTTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAACA TCTTTGTGGC CTTGATTATC ATAACTTTCC AGGAGCAGGG TGACAAAATA 
ATGGAGGAGT ACAGTTTGGA AAAGAATGAG AGAGCTTGCA TAGACTTTGC TATCAGTGCC 
AAGCCGCTGA CGCGCCATAT GCCGCAGAAC AAGCAGAGCT TTCAGTATCG CATGTGGCAG 
TTTGTGGTGT CTCCCCCCTT TGAATACACC ATCATGGCTC TCATCGCACT CAATACGATT 
GTGTTAATGA TGAAGTTCCA CGGATCTTCT AATACATATG AAAATGTGCT GAAAACATTC 
AACATGGTCT TCACCTCCCT CTTCTCTCTT GAATGCCTTC TCAAAATAAT GGCTTTCGGT 
GCTCCGAATT ATTTCCGGGA CGCCTGGAAC ATCTTTGATT TTGTGACGGT TCTTGGGAGC 
ATCACGGATA TTGTAGTGAC TGAGCTCTGG GACAGCATCA TCAACCTCAG CTTCCTCCGT 
CTGTTTCGAG CTGCCCGACT TATTAAACTC CTGCGGCAGG GATACACCAT TCGTATTCTA 
CTGTGGACCT TCGTCCAGTC CTTCAAGGCT CTACCCTACG TCTGCCTCCT GATCGCTATG 
CTGTTCTTTA TTTATGCCAT CATCGGCATG CAGGTGTTCG GTAACATTGC CTTGGATGAA 
GAAGACGACG ACACAGCTAT AACCGAGCAC AATAATTTCC GCAACTTTTT CCAAGCTCTT 
ATGCTTCTCT TCCGCAGTGC CACGGGCGAG GCTTGGCATG AAATCATGCT GTCCTGTTTG 
AGTGGGAGAC CATGTGACGT CGGGTCCGGA TTAAAGGAGA AGGAGTGCGG CAGTGAATTT 
GCTTATTTTT ACTTTGTGTC GTTCATATTC CTCTGTTCCT TCCTGATGTT AAACCTGTTT 
GTTGCTGTCA TCATGGACAA CTTTGAATAT CTGACCCGTG ATTCATCCAT CTTGGGCCCT 
CATCACCTTG ATGAATACGT GCGAGTATGG GCGGAGTATG ATCCTGCCGC TTGGGGTCGC 
ATGAAATACA CAGACTTGTA TGAGATGCTC AGACTTATCT CGCCTCCCTT GGGTCTGGGG 
AAGAAATGCC CTGCTCGAGT TGCTTATAAG AGGTTACTCC GAATGGATCT TCCTGTTGCT 
GACGATAACA CTGTGCATTT CAACTCTACT CTGATGGCTC TGATTAGAAC TGCGTTGGAC 
ATCAAGATTG CCAAGGGTGG GGCAGACAAG CAGCAGATGG ACAGTCAACT CAGAAAGGAA 
ATGATGGCTA TTTGGCCAAA CATGTCCCAG AAAACACTGG ACCTATTGGT GACCCCACAT 
AAGTCTACTG ACCTCACTGT TGGAAAGATC TATGCTGCCA TGATGATTAT GGAGTATTAC 
AGGCAGAGCA AAGCAAAAAG GCTTCAGGCT CTGCAGGAGG AACAGAACCG CACCCCTCTC 
ATGTTCCAGC GAATGGAACC ACCATCGCCC ACACAGGATG GACCCTTAGG AATCAATGCT 
TTGCCGTCCT CCCAAGCTGG AGAAATGGGG CTGCCTGAAG GGGGCATGAA GGAGAGCCAG 
TCTTGGATGA CCCAACGGGC CCAGCAGATG TTTCAGAAAA CAGGGACCTG GAGCCCAGAG 
AGGGCGCGCA CACAGGAAAA TACCAACAGC TGGCCAAATT CCCAACAGTC TGTAGAGATG 
AGTGACATGG GTCGGGACGG TTACTCAGAC AGCGAGCAGT ACCTGGTCAT GGAGGGACAT 
GGAAGAGCCG CTTCAATGCC ACGGCTTCCA GCTGATAACC AGCAGCGAAG GAAAACCCGA 
ACCAGAGGGA GTAATCTCAG TACTATTACG GACGGGAGTC CTATGAAACG TTCCGCCTCC 
ATGCTCGGCC ACTCCCAGTC TAAAGGCATC AGATTGGATG AGTATTCATT AGAGAGGGTT 
ACACCTGAGG AAGGACCTCG CCATGGGCAC CAACGCAGGA GGGAGAGGGG ACACAGGGTC 
TCTGACAGAT CCCTCACCCG ATATGTGGAT CTGGAGTCAG GATTGGGTAC AGAGCTCAGC 
ACTACAACAC AGTCATGTGA TCTCCCCACT GAAGAGCGTG GACGCACTCG AGATCGGAAG 
CACCACCATC ACCACCACCA CCATCATCAT CACCATCATC ACCACCCCAG CACTGAACGG 
GAGAGATTAG AGTACGGAAG GCCAAGCTCT CGCTCCCCCA GCACAGGGCG AGATTATGCT 
CCACACAGAC AGGGCAGTAG TTCAGTGGAT GGCAGCCCCG TGCCCTCAAC CTCTGGGGCC 
AGCACACCCC GCAGAGGAAG GCGACAGCTG CCCCAGCTGC CAACAACTCC CAGGCCACGA 
GTTACTTATT CTCCAGTGGT CCGCCGGCCT GCCAGCGTTT GCTCTCCCCA GCAGCAGTCC 
TTGTGCAGAA TGACCACCCC TATCCAACGG AGATATCCCC AGACTGAGAA CCGTCCAAGC 
AGCAATGAAA GACGGTCCGT TAACAATGAT TCCCCCCGAT CCCATCGTCA CACAAGAGGA 
CACTGCACGG AAGTCCCCCG CGCTGGATGC AGGCACAGCG GCCCTTATCG AGATTCCTCT 
TTTGAGGACC CTCATCCCTG CGATCAGCGC TCCTCCAGGA CTGCCGAGTC TGGGGGGGTA 
CCCCCTCTCT CACCGCGCGG GCGCAGGTTG CCCAATGGTT ACTCTCATGG GACCCCGCGA 
TCCACAAAAT CACGTCATGA ACGTTACAGT GAGAGTGAGG AGGATGACTG GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755315.1
CDS	9..6905
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899455.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899455.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCA GGTGGAAGCA AAGGAAAGGA TATAAGTACC 
ATCAAGTCAC TGCGTGTTCT GCGTGTCCTG CGTCCTCTGA AAACAATCAA ACGGCTTCCC 
AAGCTAAAGG CCGTGTTCGA CTGCGTAGTC AACTCTCTGA AGAACGTTCT GAACATTCTC 
ATTGTCTACA TGCTCTTCAT GTTCATCTTC GCTGTTGTAG CCGTCCAGCT TTTCAAGGGG 
AAGTTCTTTT ATTGTACAGA TGAGTCCAAG GAATTCCAGA GAGACTGCAG GGGAGAATAC 
CTGGTCTATG ACAAAAGTGA AGTTAAAGCA CAGAAACGAG AGTGGAAGAA ATGGCCGTTC 
CATTATGACA ATGTGCTATG GGCACTTCTC ACCCTCTTTA CTGTATCTAC AGGGGAAGGT 
TGGCCAGATG TGCTCAGACA TTCTGTGGAT GCCACCTATG AGGATCAGGG TCCCAGGCCT 
GGTTTTCGGA TGGAGATGTC TATATTTTAT GTGGTCTACT TTGTGGTCTT CCCTTTCTTC 
TTTGTCAACA TCTTTGTGGC CTTGATTATC ATAACTTTCC AGGAGCAGGG TGACAAAATA 
ATGGAGGAGT ACAGTTTGGA AAAGAATGAG AGAGCTTGCA TAGACTTTGC TATCAGTGCC 
AAGCCGCTGA CGCGCCATAT GCCGCAGAAC AAGCAGAGCT TTCAGTATCG CATGTGGCAG 
TTTGTGGTGT CTCCCCCCTT TGAATACACC ATCATGGCTC TCATCGCACT CAATACGATT 
GTGTTAATGA TGAAGTTCCA CGGATCTTCT AATACATATG AAAATGTGCT GAAAACATTC 
AACATGGTCT TCACCTCCCT CTTCTCTCTT GAATGCCTTC TCAAAATAAT GGCTTTCGGT 
GCTCCGAATT ATTTCCGGGA CGCCTGGAAC ATCTTTGATT TTGTGACGGT TCTTGGGAGC 
ATCACGGATA TTGTAGTGAC TGAGCTCTGG GACAGCATCA TCAACCTCAG CTTCCTCCGT 
CTGTTTCGAG CTGCCCGACT TATTAAACTC CTGCGGCAGG GATACACCAT TCGTATTCTA 
CTGTGGACCT TCGTCCAGTC CTTCAAGGCT CTACCCTACG TCTGCCTCCT GATCGCTATG 
CTGTTCTTTA TTTATGCCAT CATCGGCATG CAGGTGTTCG GTAACATTGC CTTGGATGAA 
GAAGACGACG ACACAGCTAT AACCGAGCAC AATAATTTCC GCAACTTTTT CCAAGCTCTT 
ATGCTTCTCT TCCGCAGTGC CACGGGCGAG GCTTGGCATG AAATCATGCT GTCCTGTTTG 
AGTGGGAGAC CATGTGACGT CGGGTCCGGA TTAAAGGAGA AGGAGTGCGG CAGTGAATTT 
GCTTATTTTT ACTTTGTGTC GTTCATATTC CTCTGTTCCT TCCTGATGTT AAACCTGTTT 
GTTGCTGTCA TCATGGACAA CTTTGAATAT CTGACCCGTG ATTCATCCAT CTTGGGCCCT 
CATCACCTTG ATGAATACGT GCGAGTATGG GCGGAGTATG ATCCTGCCGC TTGGGGTCGC 
ATGAAATACA CAGACTTGTA TGAGATGCTC AGACTTATCT CGCCTCCCTT GGGTCTGGGG 
AAGAAATGCC CTGCTCGAGT TGCTTATAAG AGGTTACTCC GAATGGATCT TCCTGTTGCT 
GACGATAACA CTGTGCATTT CAACTCTACT CTGATGGCTC TGATTAGAAC TGCGTTGGAC 
ATCAAGATTG CCAAGGGTGG GGCAGACAAG CAGCAGATGG ACAGTCAACT CAGAAAGGAA 
ATGATGGCTA TTTGGCCAAA CATGTCCCAG AAAACACTGG ACCTATTGGT GACCCCACAT 
AAGTCTACTG ACCTCACTGT TGGAAAGATC TATGCTGCCA TGATGATTAT GGAGTATTAC 
AGGCAGAGCA AAGCAAAAAG GCTTCAGGCT CTGCAGGAGG AACAGAACCG CACCCCTCTC 
ATGTTCCAGC GAATGGAACC ACCATCGCCC ACACAGGATG GACCCTTAGG AATCAATGCT 
TTGCCGTCCT CCCAAGCTGG AGAAATGGGG CTGCCTGAAG GGGGCATGAA GGAGAGCCAG 
TCTTGGATGA CCCAACGGGC CCAGCAGATG TTTCAGAAAA CAGGGACCTG GAGCCCAGAG 
AGGGCGCGCA CACAGGAAAA TACCAACAGC TGGCCAAATT CCCAACAGTC TGTAGAGATG 
AGTGACATGG GTCGGGACGG TTACTCAGAC AGCGAGCAGT ACCTGGTCAT GGAGGGACAT 
GGAAGAGCCG CTTCAATGCC ACGGCTTCCA GCTGATAACC AGCAGCGAAG GAAAACCCGA 
ACCAGAGGGA GTAATCTCAG TACTATTACG GACGGGAGTC CTATGAAACG TTCCGCCTCC 
ATGCTCGGCC ACTCCCAGTC TAAAGGCATC AGATTGGATG AGTATTCATT AGAGAGGGTT 
ACACCTGAGG AAGGACCTCG CCATGGGCAC CAACGCAGGA GGGAGAGGGG ACACAGGGTC 
TCTGACAGAT CCCTCACCCG ATATGTGGAT CTGGAGTCAG GATTGGGTAC AGAGCTCAGC 
ACTACAACAC AGTCATGTGA TCTCCCCACT GAAGAGCGTG GACGCACTCG AGATCGGAAG 
CACCACCATC ACCACCACCA CCATCATCAT CACCATCATC ACCACCCCAG CACTGAACGG 
GAGAGATTAG AGTACGGAAG GCCAAGCTCT CGCTCCCCCA GCACAGGGCG AGATTATGCT 
CCACACAGAC AGGGCAGTAG TTCAGTGGAT GGCAGCCCCG TGCCCTCAAC CTCTGGGGCC 
AGCACACCCC GCAGAGGAAG GCGACAGCTG CCCCAGCTGC CAACAACTCC CAGGCCACGA 
GTTACTTATT CTCCAGTGGT CCGCCGGCCT GCCAGCGTTT GCTCTCCCCA GCAGCAGTCC 
TTGTGCAGAA TGACCACCCC TATCCAACGG AGATATCCCC AGACTGAGAA CCGTCCAAGC 
AGCAATGAAA GACGGTCCGT TAACAATGAT TCCCCCCGAT CCCATCGTCA CACAAGAGGA 
CACTGCACGG AAGTCCCCCG CGCTGGATGC AGGCACAGCG GCCCTTATCG AGATTCCTCT 
TTTGAGGACC CTCATCCCTG CGATCAGCGC TCCTCCAGGA CTGCCGAGTC TGGGGGGGTA 
CCCCCTCTCT CACCGCGCGG GCGCAGGTTG CCCAATGGTT ACTCTCATGG GACCCCGCGA 
TCCACAAAAT CACGTCATGA ACGTTACAGT GAGAGTGAGG AGGATGACTG GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899456.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755316.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X6, mRNA.

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CloneID	OXa55406
Clone ID Related Accession (Same CDS sequence)	XM_031899456.1
Accession Version	XM_031899456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGACTATT 
ACGGACGGGA GTCCTATGAA ACGTTCCGCC TCCATGCTCG GCCACTCCCA GTCTAAAGGC 
ATCAGATTGG ATGAGTATTC ATTAGAGAGG GTTACACCTG AGGAAGGACC TCGCCATGGG 
CACCAACGCA GGAGGGAGAG GGGACACAGG GTCTCTGACA GATCCCTCAC CCGATATGTG 
GATCTGGAGT CAGGATTGGG TACAGAGCTC AGCACTACAA CACAGTCATG TGATCTCCCC 
ACTGAAGAGC GTGGACGCAC TCGAGATCGG AAGCACCACC ATCACCACCA CCACCATCAT 
CATCACCATC ATCACCACCC CAGCACTGAA CGGGAGAGAT TAGAGTACGG AAGGCCAAGC 
TCTCGCTCCC CCAGCACAGG GCGAGATTAT GCTCCACACA GACAGGGCAG TAGTTCAGTG 
GATGGCAGCC CCGTGCCCTC AACCTCTGGG GCCAGCACAC CCCGCAGAGG AAGGCGACAG 
CTGCCCCAGC TGCCAACAAC TCCCAGGCCA CGAGTTACTT ATTCTCCAGT GGTCCGCCGG 
CCTGCCAGCG TTTGCTCTCC CCAGCAGCAG TCCTTGTGCA GAATGACCAC CCCTATCCAA 
CGGAGATATC CCCAGACTGA GAACCGTCCA AGCAGCAATG AAAGACGGTC CGTTAACAAT 
GATTCCCCCC GATCCCATCG TCACACAAGA GGACACTGCA CGGAAGTCCC CCGCGCTGGA 
TGCAGGCACA GCGGCCCTTA TCGAGATTCC TCTTTTGAGG ACCCTCATCC CTGCGATCAG 
CGCTCCTCCA GGACTGCCGA GTCTGGGGGG GTACCCCCTC TCTCACCGCG CGGGCGCAGG 
TTGCCCAATG GTTACTCTCA TGGGACCCCG CGATCCACAA AATCACGTCA TGAACGTTAC 
AGTGAGAGTG AGGAGGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755316.1
CDS	9..6878
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_031899456.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899456.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGACTATT 
ACGGACGGGA GTCCTATGAA ACGTTCCGCC TCCATGCTCG GCCACTCCCA GTCTAAAGGC 
ATCAGATTGG ATGAGTATTC ATTAGAGAGG GTTACACCTG AGGAAGGACC TCGCCATGGG 
CACCAACGCA GGAGGGAGAG GGGACACAGG GTCTCTGACA GATCCCTCAC CCGATATGTG 
GATCTGGAGT CAGGATTGGG TACAGAGCTC AGCACTACAA CACAGTCATG TGATCTCCCC 
ACTGAAGAGC GTGGACGCAC TCGAGATCGG AAGCACCACC ATCACCACCA CCACCATCAT 
CATCACCATC ATCACCACCC CAGCACTGAA CGGGAGAGAT TAGAGTACGG AAGGCCAAGC 
TCTCGCTCCC CCAGCACAGG GCGAGATTAT GCTCCACACA GACAGGGCAG TAGTTCAGTG 
GATGGCAGCC CCGTGCCCTC AACCTCTGGG GCCAGCACAC CCCGCAGAGG AAGGCGACAG 
CTGCCCCAGC TGCCAACAAC TCCCAGGCCA CGAGTTACTT ATTCTCCAGT GGTCCGCCGG 
CCTGCCAGCG TTTGCTCTCC CCAGCAGCAG TCCTTGTGCA GAATGACCAC CCCTATCCAA 
CGGAGATATC CCCAGACTGA GAACCGTCCA AGCAGCAATG AAAGACGGTC CGTTAACAAT 
GATTCCCCCC GATCCCATCG TCACACAAGA GGACACTGCA CGGAAGTCCC CCGCGCTGGA 
TGCAGGCACA GCGGCCCTTA TCGAGATTCC TCTTTTGAGG ACCCTCATCC CTGCGATCAG 
CGCTCCTCCA GGACTGCCGA GTCTGGGGGG GTACCCCCTC TCTCACCGCG CGGGCGCAGG 
TTGCCCAATG GTTACTCTCA TGGGACCCCG CGATCCACAA AATCACGTCA TGAACGTTAC 
AGTGAGAGTG AGGAGGATGA CTGGTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_031899457.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755317.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X7, mRNA.

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CloneID	OXa55407
Clone ID Related Accession (Same CDS sequence)	XM_031899457.1
Accession Version	XM_031899457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755317.1
CDS	9..6395
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSSNQRGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMKYTDLYEMLRLISPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQ

Target ORF information:

RefSeq Version	XM_031899457.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899457.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTCTTCGGC GACTGTGCCA TTACATAGTC 
ACTATGCGAT ACTTTGAAAT GTGCATCTTG ATGGTGATCG CAATGAGCAG TATAGCCCTC 
GCTGCTGAAG ACCCAGTGCA GCCAGATGCC CCCAGGAATA ACGTCTTGCG TTATTTTGAC 
TACGTCTTTA CGGGAGTCTT CACTTTTGAG ATGGTTATCA AGATGGTCGA TCTGGGCCTC 
ATTCTACATC AGGGAGCCTA CTTCCGGGAT CTGTGGAACT TTTTAGATTT CATCGTCGTC 
TGCGGTGCTC TAGTGGCCTT CGCCTTTTCG AGCAATCAGC GAGGTGGAAG CAAAGGAAAG 
GATATAAGTA CCATCAAGTC ACTGCGTGTT CTGCGTGTCC TGCGTCCTCT GAAAACAATC 
AAACGGCTTC CCAAGCTAAA GGCCGTGTTC GACTGCGTAG TCAACTCTCT GAAGAACGTT 
CTGAACATTC TCATTGTCTA CATGCTCTTC ATGTTCATCT TCGCTGTTGT AGCCGTCCAG 
CTTTTCAAGG GGAAGTTCTT TTATTGTACA GATGAGTCCA AGGAATTCCA GAGAGACTGC 
AGGGGAGAAT ACCTGGTCTA TGACAAAAGT GAAGTTAAAG CACAGAAACG AGAGTGGAAG 
AAATGGCCGT TCCATTATGA CAATGTGCTA TGGGCACTTC TCACCCTCTT TACTGTATCT 
ACAGGGGAAG GTTGGCCAGA TGTGCTCAGA CATTCTGTGG ATGCCACCTA TGAGGATCAG 
GGTCCCAGGC CTGGTTTTCG GATGGAGATG TCTATATTTT ATGTGGTCTA CTTTGTGGTC 
TTCCCTTTCT TCTTTGTCAA CATCTTTGTG GCCTTGATTA TCATAACTTT CCAGGAGCAG 
GGTGACAAAA TAATGGAGGA GTACAGTTTG GAAAAGAATG AGAGAGCTTG CATAGACTTT 
GCTATCAGTG CCAAGCCGCT GACGCGCCAT ATGCCGCAGA ACAAGCAGAG CTTTCAGTAT 
CGCATGTGGC AGTTTGTGGT GTCTCCCCCC TTTGAATACA CCATCATGGC TCTCATCGCA 
CTCAATACGA TTGTGTTAAT GATGAAGTTC CACGGATCTT CTAATACATA TGAAAATGTG 
CTGAAAACAT TCAACATGGT CTTCACCTCC CTCTTCTCTC TTGAATGCCT TCTCAAAATA 
ATGGCTTTCG GTGCTCCGAA TTATTTCCGG GACGCCTGGA ACATCTTTGA TTTTGTGACG 
GTTCTTGGGA GCATCACGGA TATTGTAGTG ACTGAGCTCT GGGACAGCAT CATCAACCTC 
AGCTTCCTCC GTCTGTTTCG AGCTGCCCGA CTTATTAAAC TCCTGCGGCA GGGATACACC 
ATTCGTATTC TACTGTGGAC CTTCGTCCAG TCCTTCAAGG CTCTACCCTA CGTCTGCCTC 
CTGATCGCTA TGCTGTTCTT TATTTATGCC ATCATCGGCA TGCAGGTGTT CGGTAACATT 
GCCTTGGATG AAGAAGACGA CGACACAGCT ATAACCGAGC ACAATAATTT CCGCAACTTT 
TTCCAAGCTC TTATGCTTCT CTTCCGCAGT GCCACGGGCG AGGCTTGGCA TGAAATCATG 
CTGTCCTGTT TGAGTGGGAG ACCATGTGAC GTCGGGTCCG GATTAAAGGA GAAGGAGTGC 
GGCAGTGAAT TTGCTTATTT TTACTTTGTG TCGTTCATAT TCCTCTGTTC CTTCCTGATG 
TTAAACCTGT TTGTTGCTGT CATCATGGAC AACTTTGAAT ATCTGACCCG TGATTCATCC 
ATCTTGGGCC CTCATCACCT TGATGAATAC GTGCGAGTAT GGGCGGAGTA TGATCCTGCC 
GCTTGGGGTC GCATGAAATA CACAGACTTG TATGAGATGC TCAGACTTAT CTCGCCTCCC 
TTGGGTCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTATA AGAGGTTACT CCGAATGGAT 
CTTCCTGTTG CTGACGATAA CACTGTGCAT TTCAACTCTA CTCTGATGGC TCTGATTAGA 
ACTGCGTTGG ACATCAAGAT TGCCAAGGGT GGGGCAGACA AGCAGCAGAT GGACAGTCAA 
CTCAGAAAGG AAATGATGGC TATTTGGCCA AACATGTCCC AGAAAACACT GGACCTATTG 
GTGACCCCAC ATAAGTCTAC TGACCTCACT GTTGGAAAGA TCTATGCTGC CATGATGATT 
ATGGAGTATT ACAGGCAGAG CAAAGCAAAA AGGCTTCAGG CTCTGCAGGA GGAACAGAAC 
CGCACCCCTC TCATGTTCCA GCGAATGGAA CCACCATCGC CCACACAGGA TGGACCCTTA 
GGAATCAATG CTTTGCCGTC CTCCCAAGCT GGAGAAATGG GGCTGCCTGA AGGGGGCATG 
AAGGAGAGCC AGTCTTGGAT GACCCAACGG GCCCAGCAGA TGTTTCAGAA AACAGGGACC 
TGGAGCCCAG AGAGGGCGCG CACACAGGAA AATACCAACA GCTGGCCAAA TTCCCAACAG 
TCTGTAGAGA TGAGTGACAT GGGTCGGGAC GGTTACTCAG ACAGCGAGCA GTACCTGGTC 
ATGGAGGGAC ATGGAAGAGC CGCTTCAATG CCACGGCTTC CAGCTGATAA CCAGCAGCGA 
AGGAAAACCC GAACCAGAGG GAGTAATCTC AGTACTATTA CGGACGGGAG TCCTATGAAA 
CGTTCCGCCT CCATGCTCGG CCACTCCCAG TCTAAAGGCA TCAGATTGGA TGAGTATTCA 
TTAGAGAGGG TTACACCTGA GGAAGGACCT CGCCATGGGC ACCAACGCAG GAGGGAGAGG 
GGACACAGGG TCTCTGACAG ATCCCTCACC CGATATGTGG ATCTGGAGTC AGGATTGGGT 
ACAGAGCTCA GCACTACAAC ACAGTCATGT GATCTCCCCA CTGAAGAGCG TGGACGCACT 
CGAGATCGGA AGCACCACCA TCACCACCAC CACCATCATC ATCACCATCA TCACCACCCC 
AGCACTGAAC GGGAGAGATT AGAGTACGGA AGGCCAAGCT CTCGCTCCCC CAGCACAGGG 
CGAGATTATG CTCCACACAG ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1a ( XM_018092693.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948182.1 Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), mRNA.

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CloneID	OXa43582
Clone ID Related Accession (Same CDS sequence)	XM_018092693.1
Accession Version	XM_018092693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTGCATTCC TTTCTCTTAT CACAGCCAAT 
AAGATTGTTG CAGTCTCCCC GCTTCCTAAA AAAGACGATG AGAAAAAAGA AGAAGAGGAG 
GATGATGATG ATGGAGAAAA GGGCCCTAAG CCGATGCCCC CCTACAGCTC CATGTTTATT 
TTGTCTACTA CTAACCCTCT TCGGCGACTG TGCCATTACA TAGTCACTAT GCGATACTTT 
GAAATGTGCA TCTTGATGGT GATCGCAATG AGCAGTATAG CCCTCGCTGC TGAAGACCCA 
GTGCAGCCAG ATGCCCCCAG GAATAACGTC TTGCGTTATT TTGACTACGT CTTTACGGGA 
GTCTTCACTT TTGAGATGGT TATCAAGATG GTCGATCTGG GCCTCATTCT ACATCAGGGA 
GCCTACTTCC GGGATCTGTG GAACTTTTTA GATTTCATCG TCGTCTGCGG TGCTCTAGTG 
GCCTTCGCCT TTTCAGGTGG AAGCAAAGGA AAGGATATAA GTACCATCAA GTCACTGCGT 
GTTCTGCGTG TCCTGCGTCC TCTGAAAACA ATCAAACGGC TTCCCAAGCT AAAGGCCGTG 
TTCGACTGCG TAGTCAACTC TCTGAAGAAC GTTCTGAACA TTCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTCGCTGT TGTAGCCGTC CAGCTTTTCA AGGGGAAGTT CTTTTATTGT 
ACAGATGAGT CCAAGGAATT CCAGAGAGAC TGCAGGGGAG AATACCTGGT CTATGACAAA 
AGTGAAGTTA AAGCACAGAA ACGAGAGTGG AAGAAATGGC CGTTCCATTA TGACAATGTG 
CTATGGGCAC TTCTCACCCT CTTTACTGTA TCTACAGGGG AAGGTTGGCC AGATGTGCTC 
AGACATTCTG TGGATGCCAC CTATGAGGAT CAGGGTCCCA GGCCTGGTTT TCGGATGGAG 
ATGTCTATAT TTTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAACATCTTT 
GTGGCCTTGA TTATCATAAC TTTCCAGGAG CAGGGTGACA AAATAATGGA GGAGTACAGT 
TTGGAAAAGA ATGAGAGAGC TTGCATAGAC TTTGCTATCA GTGCCAAGCC GCTGACGCGC 
CATATGCCGC AGAACAAGCA GAGCTTTCAG TATCGCATGT GGCAGTTTGT GGTGTCTCCC 
CCCTTTGAAT ACACCATCAT GGCTCTCATC GCACTCAATA CGATTGTGTT AATGATGAAG 
TTCCACGGAT CTTCTAATAC ATATGAAAAT GTGCTGAAAA CATTCAACAT GGTCTTCACC 
TCCCTCTTCT CTCTTGAATG CCTTCTCAAA ATAATGGCTT TCGGTGCTCC GAATTATTTC 
CGGGACGCCT GGAACATCTT TGATTTTGTG ACGGTTCTTG GGAGCATCAC GGATATTGTA 
GTGACTGAGC TCTGGGACAG CATCATCAAC CTCAGCTTCC TCCGTCTGTT TCGAGCTGCC 
CGACTTATTA AACTCCTGCG GCAGGGATAC ACCATTCGTA TTCTACTGTG GACCTTCGTC 
CAGTCCTTCA AGGCTCTACC CTACGTCTGC CTCCTGATCG CTATGCTGTT CTTTATTTAT 
GCCATCATCG GCATGCAGGT GTTCGGTAAC ATTGCCTTGG ATGAAGAAGA CGACGACACA 
GCTATAACCG AGCACAATAA TTTCCGCAAC TTTTTCCAAG CTCTTATGCT TCTCTTCCGC 
AGTGCCACGG GCGAGGCTTG GCATGAAATC ATGCTGTCCT GTTTGAGTGG GAGACCATGT 
GACGTCGGGT CCGGATTAAA GGAGAAGGAG TGCGGCAGTG AATTTGCTTA TTTTTACTTT 
GTGTCGTTCA TATTCCTCTG TTCCTTCCTG ATGTTAAACC TGTTTGTTGC TGTCATCATG 
GACAACTTTG AATATCTGAC CCGTGATTCA TCCATCTTGG GCCCTCATCA CCTTGATGAA 
TACGTGCGAG TATGGGCGGA GTATGATCCT GCCGCTTGCG GGCGTATTCA TTATAAGGAC 
ATGTACAGTT TGTTACGGGT TATCTCCCCT CCGCTGGGGT TGGGGAAGAA ATGCCCCCAC 
AGGGTGGCCT GCAAGAGGTT ACTCCGAATG GATCTTCCTG TTGCTGACGA TAACACTGTG 
CATTTCAACT CTACTCTGAT GGCTCTGATT AGAACTGCGT TGGACATCAA GATTGCCAAG 
GGTGGGGCAG ACAAGCAGCA GATGGACAGT CAACTCAGAA AGGAAATGAT GGCTATTTGG 
CCAAACATGT CCCAGAAAAC ACTGGACCTA TTGGTGACCC CACATAAGTC TACTGACCTC 
ACTGTTGGAA AGATCTATGC TGCCATGATG ATTATGGAGT ATTACAGGCA GAGCAAAGCA 
AAAAGGCTTC AGGCTCTGCA GGAGGAACAG AACCGCACCC CTCTCATGTT CCAGCGAATG 
GAACCACCAT CGCCCACACA GGATGGACCC TTAGGAATCA ATGCTTTGCC GTCCTCCCAA 
GCTGGAGAAA TGGGGCTGCC TGAAGGGGGC ATGAAGGAGA GCCAGTCTTG GATGACCCAA 
CGGGCCCAGC AGATGTTTCA GAAAACAGGG ACCTGGAGCC CAGAGAGGGC GCGCACACAG 
GAAAATACCA ACAGCTGGCC AAATTCCCAA CAGTCTGTAG AGATGAGTGA CATGGGTCGG 
GACGGTTACT CAGACAGCGA GCAGTACCTG GTCATGGAGG GACATGGAAG AGCCGCTTCA 
ATGCCACGGC TTCCAGCTGA TAACCAGCAG CGAAGGAAAA CCCGAACCAG AGGGAGTAAT 
CTCAGTACTA TTACGGACGG GAGTCCTATG AAACGTTCCG CCTCCATGCT CGGCCACTCC 
CAGTCTAAAG GCATCAGATT GGATGAGTAT TCATTAGAGA GGGTTACACC TGAGGAAGGA 
CCTCGCCATG GGCACCAACG CAGGAGGGAG AGGGGACACA GGGTCTCTGA CAGATCCCTC 
ACCCGATATG TGGATCTGGA GTCAGGATTG GGTACAGAGC TCAGCACTAC AACACAGTCA 
TGTGATCTCC CCACTGAAGA GCGTGGACGC ACTCGAGATC GGAAGCACCA CCATCACCAC 
CACCACCATC ATCATCACCA TCATCACCAC CCCAGCACTG AACGGGAGAG ATTAGAGTAC 
GGAAGGCCAA GCTCTCGCTC CCCCAGCACA GGGCGAGATT ATGCTCCACA CAGACAGGGC 
AGTAGTTCAG TGGATGGCAG CCCCGTGCCC TCAACCTCTG GGGCCAGCAC ACCCCGCAGA 
GGAAGGCGAC AGCTGCCCCA GCTGCCAACA ACTCCCAGGC CACGAGTTAC TTATTCTCCA 
GTGGTCCGCC GGCCTGCCAG CGTTTGCTCT CCCCAGCAGC AGTCCTTGTG CAGAATGACC 
ACCCCTATCC AACGGAGATA TCCCCAGACT GAGAACCGTC CAAGCAGCAA TGAAAGACGG 
TCCGTTAACA ATGATTCCCC CCGATCCCAT CGTCACACAA GAGGACACTG CACGGAAGTC 
CCCCGCGCTG GATGCAGGCA CAGCGGCCCT TATCGAGATT CCTCTTTTGA GGACCCTCAT 
CCCTGCGATC AGCGCTCCTC CAGGACTGCC GAGTCTGGGG GGGTACCCCC TCTCTCACCG 
CGCGGGCGCA GGTTGCCCAA TGGTTACTCT CATGGGACCC CGCGATCCAC AAAATCACGT 
CATGAACGTT ACAGTGAGAG TGAGGAGGAT GACTGGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948182.1
CDS	9..7070
Translation	MILATIIANCIVLAMEQHLPVGDKTPMSERLDDTEPYFIGIFCFETGIKIIALGFAFHKGSYLRNGWNIMDFVVVLSGIVASTGPEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLDFYIGKFHTSCYDMAGEDIREEVLCGNDSSSRRCPENTQCLSKWEGPNNGITQFDNILFAILTVFQCITMEGWTDILYYSNDVFGSAFNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYMEWISKAEVILAEDDSDIEQRLPYDGALRRPTIKKNKTELLNAEEGEEAIGDIASVGSPFARASIKSAKMENSSYMHKKERRIRFLIRRMVKTQAFYWVVLGLVALNTLCVAIVHYGQDDRLSEFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCAVITGSIFEVVWAMVKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVISLLSSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEKTPATNFDTFPAAIMTVFQILTGEDWNAVMYDGIKSQGGVKTGMVFSIYFIILTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLAMQKAKEVAEVSPLSATNMSIAAKEQQKNQKTSMSVWEQRTNEIRRQNLMNSREALYSELDPEERWKLSFMRPDVKTHLDRPLVVDPQENRNNNTNKTRPGEGRNPIEQRLSQQRAEDYLRKQARFQERGGRGYDMGQEPGRRPRIKEQDIIRDGQDLGMQDRTGEMDGGIRPPQDPHRRHRGGGSKESRSGSPLPRGDGDHRRHRPHRRTGEDNEDAGGGGGGGGRGGGGGGDRRPRHGRDGMRVREGGGGESEGPEGERRRRHRHAAQSTYESDAKRDDRERRHRRRKEPTAPTPGSGGPNLSTTRPIQDRSRQENQNQQPENMDNIRNTKLVTSEPPAGGGVGGLNHSPAKIGNHTNCANNNSVPTGNATPRRTPHPTNSGPPPSAPDPSHTFSNPTETAKGTKTTEHTTVDIPPPFPPPVENAIGQANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPAFLSLITANKIVAVSPLPKKDDEKKEEEEDDDDGEKGPKPMPPYSSMFILSTTNPLRRLCHYIVTMRYFEMCILMVIAMSSIALAAEDPVQPDAPRNNVLRYFDYVFTGVFTFEMVIKMVDLGLILHQGAYFRDLWNFLDFIVVCGALVAFAFSGGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGKFFYCTDESKEFQRDCRGEYLVYDKSEVKAQKREWKKWPFHYDNVLWALLTLFTVSTGEGWPDVLRHSVDATYEDQGPRPGFRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKIMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMALIALNTIVLMMKFHGSSNTYENVLKTFNMVFTSLFSLECLLKIMAFGAPNYFRDAWNIFDFVTVLGSITDIVVTELWDSIINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDEEDDDTAITEHNNFRNFFQALMLLFRSATGEAWHEIMLSCLSGRPCDVGSGLKEKECGSEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDSQLRKEMMAIWPNMSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRLQALQEEQNRTPLMFQRMEPPSPTQDGPLGINALPSSQAGEMGLPEGGMKESQSWMTQRAQQMFQKTGTWSPERARTQENTNSWPNSQQSVEMSDMGRDGYSDSEQYLVMEGHGRAASMPRLPADNQQRRKTRTRGSNLSTITDGSPMKRSASMLGHSQSKGIRLDEYSLERVTPEEGPRHGHQRRRERGHRVSDRSLTRYVDLESGLGTELSTTTQSCDLPTEERGRTRDRKHHHHHHHHHHHHHHHPSTERERLEYGRPSSRSPSTGRDYAPHRQGSSSVDGSPVPSTSGASTPRRGRRQLPQLPTTPRPRVTYSPVVRRPASVCSPQQQSLCRMTTPIQRRYPQTENRPSSNERRSVNNDSPRSHRHTRGHCTEVPRAGCRHSGPYRDSSFEDPHPCDQRSSRTAESGGVPPLSPRGRRLPNGYSHGTPRSTKSRHERYSESEEDDWC

Target ORF information:

RefSeq Version	XM_018092693.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (cacna1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092693.1

ORF Insert Sequence:

ATGATTTTAG CAACTATCAT AGCTAACTGC ATCGTACTGG CTATGGAGCA ACACTTACCC 
GTGGGGGACA AGACACCAAT GTCGGAACGT CTGGATGACA CTGAACCCTA TTTTATTGGA 
ATTTTCTGTT TTGAGACGGG GATTAAAATC ATCGCCTTGG GATTTGCCTT TCACAAAGGA 
TCCTATTTGC GGAATGGCTG GAATATCATG GATTTTGTGG TTGTGTTATC AGGCATTGTG 
GCATCTACAG GCCCAGAGTT TGATCTGAGG ACCCTCCGAG CTGTACGTGT CCTGCGACCC 
CTCAAACTGG TTTCTGGGAT TCCAAGTTTA CAAGTTGTGC TGAAGTCGAT CATGAAAGCC 
ATGATCCCTC TGCTCCAGAT TGGACTGCTG CTCTTCTTTG CTATCCTCAT CTTTGCCATC 
ATTGGCCTCG ACTTCTACAT TGGCAAATTC CACACATCCT GTTATGACAT GGCTGGAGAA 
GATATAAGAG AGGAGGTTCT CTGCGGCAAT GACAGCTCCT CTCGTCGCTG CCCTGAAAAT 
ACGCAGTGCC TGAGCAAATG GGAGGGTCCT AATAATGGAA TCACGCAGTT TGACAACATT 
CTGTTTGCAA TTCTCACAGT ATTCCAGTGT ATAACTATGG AGGGATGGAC AGATATCCTG 
TATTATAGTA ACGATGTGTT CGGCAGCGCA TTCAACTGGC TTTATTTCAT TCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTAAATCTT GTCCTTGGTG TCCTTTCTGG GGAGTTTGCC 
AAAGAGAGGG AGCGCGTGGA GAACCGCCGA GCCTTCATGA AACTGAGGAG GCAGCAGCAG 
ATTGAGAGGG AACTGAATGG CTATATGGAA TGGATCTCTA AAGCAGAAGT CATCCTTGCT 
GAAGATGATA GTGATATTGA GCAGAGACTT CCTTATGATG GAGCCCTTCG GAGACCCACT 
ATCAAGAAGA ACAAGACAGA ACTGCTGAAT GCTGAGGAGG GGGAAGAGGC CATTGGGGAC 
ATAGCTTCTG TGGGCTCCCC TTTTGCACGT GCCAGCATTA AAAGTGCCAA GATGGAGAAC 
TCATCATACA TGCACAAGAA GGAGCGTCGC ATCCGTTTCC TGATTCGACG CATGGTGAAG 
ACTCAGGCCT TTTACTGGGT TGTTCTGGGT CTTGTGGCAC TGAACACGCT GTGCGTGGCA 
ATTGTGCACT ATGGCCAAGA TGACCGGCTT TCCGAATTTC TCTATTATGC AGAATTCATA 
TTCCTTGGGC TCTTTATGTC CGAAATGTTT ATAAAAATGT ACGGTCTTGG GACCAGGCCT 
TATTTTCATT CATCGTTTAA CTGCTTTGAC TGTGCCGTAA TCACCGGAAG CATCTTTGAG 
GTGGTTTGGG CAATGGTGAA GCCTGGAACC TCCTTTGGGA TCAGCGTCTT AAGAGCCCTT 
CGCTTGCTGC GTATCTTCAA AGTTACAAAG TATTGGGCTT CTCTCCGCAA TTTGGTGATC 
TCCCTACTCA GCTCAATGAA GTCCATTATC AGTCTGCTCT TCCTGCTCTT CCTTTTTATT 
GTTGTCTTTG CCTTATTGGG GATGCAGCTG TTTGGCGGGC AGTTCAATTT TGACGAGAAA 
ACTCCTGCTA CCAATTTCGA CACCTTTCCT GCAGCAATAA TGACAGTTTT TCAGATCTTA 
ACTGGGGAAG ACTGGAATGC AGTGATGTAT GATGGCATCA AGTCTCAGGG TGGAGTGAAA 
ACTGGCATGG TATTTTCCAT CTATTTCATC ATCCTTACGC TTTTTGGGAA CTACACTCTG 
CTGAATGTCT TCTTGGCCAT CGCTGTGGAC AATCTTGCTA ATGCTCAGGA GCTGACGAAG 
GATGAACAAG AAGAAGAAGA GGCAGCCAAT CAGAAGCTGG CCATGCAAAA AGCAAAAGAA 
GTAGCAGAGG TCAGCCCCTT GTCTGCTACC AACATGTCCA TTGCTGCAAA AGAGCAACAG 
AAGAACCAAA AGACTTCTAT GTCTGTTTGG GAGCAGCGCA CAAATGAGAT CCGCCGACAA 
AACTTGATGA ATAGCAGGGA GGCTTTATAC AGTGAGCTTG ATCCAGAGGA GCGTTGGAAG 
CTCTCCTTCA TGAGGCCGGA TGTCAAGACT CATTTGGATC GGCCACTGGT GGTTGACCCC 
CAGGAGAACC GCAACAACAA CACCAATAAA ACCCGTCCTG GTGAGGGAAG GAACCCCATA 
GAACAACGCT TATCCCAACA GCGAGCAGAA GACTATTTAA GAAAACAAGC ACGATTTCAG 
GAACGTGGTG GGAGGGGATA TGACATGGGC CAAGAACCTG GGCGCCGTCC TCGAATCAAG 
GAGCAGGATA TAATACGGGA TGGGCAGGAC CTAGGGATGC AAGACAGGAC AGGAGAAATG 
GATGGAGGGA TTCGTCCCCC ACAGGATCCA CACCGGCGAC ACCGGGGTGG TGGGAGCAAG 
GAAAGTCGAA GTGGTTCACC TCTGCCTAGG GGGGATGGAG ACCACAGAAG GCACAGGCCA 
CATCGCAGAA CTGGAGAGGA CAATGAGGAT GCGGGTGGTG GTGGTGGTGG AGGGGGCAGG 
GGCGGAGGTG GTGGGGGAGA CAGGAGACCT CGTCATGGTA GGGATGGAAT GAGGGTACGA 
GAAGGGGGTG GAGGAGAAAG TGAGGGTCCT GAGGGTGAGA GGAGGAGGAG GCATCGACAC 
GCGGCACAGT CCACTTACGA GAGCGATGCC AAGAGGGATG ACAGAGAGAG GAGGCATCGC 
AGAAGGAAGG AACCCACAGC CCCAACCCCA GGCTCTGGTG GGCCAAATCT TTCCACAACG 
CGACCTATTC AAGACCGTTC CCGGCAGGAG AACCAGAACC AGCAGCCTGA AAACATGGAT 
AACATTCGTA ACACCAAACT TGTGACCAGT GAGCCGCCCG CAGGTGGTGG TGTGGGTGGT 
CTCAATCACA GCCCAGCCAA AATCGGTAAC CACACCAATT GTGCCAACAA CAACAGTGTG 
CCCACTGGAA ATGCCACCCC CAGACGGACG CCTCATCCAA CCAATTCAGG CCCTCCTCCT 
TCTGCTCCTG ACCCCAGCCA CACCTTTAGC AACCCTACAG AGACTGCCAA GGGGACGAAA 
ACCACAGAAC ACACAACTGT GGATATTCCA CCCCCATTCC CTCCACCTGT GGAGAATGCT 
ATTGGGCAAG CCAATAAGAT TGTTGCAGTC TCCCCGCTTC CTAAAAAAGA CGATGAGAAA 
AAAGAAGAAG AGGAGGATGA TGATGATGGA GAAAAGGGCC CTAAGCCGAT GCCCCCCTAC 
AGCTCCATGT TTATTTTGTC TACTACTAAC CCTGCATTCC TTTCTCTTAT CACAGCCAAT 
AAGATTGTTG CAGTCTCCCC GCTTCCTAAA AAAGACGATG AGAAAAAAGA AGAAGAGGAG 
GATGATGATG ATGGAGAAAA GGGCCCTAAG CCGATGCCCC CCTACAGCTC CATGTTTATT 
TTGTCTACTA CTAACCCTCT TCGGCGACTG TGCCATTACA TAGTCACTAT GCGATACTTT 
GAAATGTGCA TCTTGATGGT GATCGCAATG AGCAGTATAG CCCTCGCTGC TGAAGACCCA 
GTGCAGCCAG ATGCCCCCAG GAATAACGTC TTGCGTTATT TTGACTACGT CTTTACGGGA 
GTCTTCACTT TTGAGATGGT TATCAAGATG GTCGATCTGG GCCTCATTCT ACATCAGGGA 
GCCTACTTCC GGGATCTGTG GAACTTTTTA GATTTCATCG TCGTCTGCGG TGCTCTAGTG 
GCCTTCGCCT TTTCAGGTGG AAGCAAAGGA AAGGATATAA GTACCATCAA GTCACTGCGT 
GTTCTGCGTG TCCTGCGTCC TCTGAAAACA ATCAAACGGC TTCCCAAGCT AAAGGCCGTG 
TTCGACTGCG TAGTCAACTC TCTGAAGAAC GTTCTGAACA TTCTCATTGT CTACATGCTC 
TTCATGTTCA TCTTCGCTGT TGTAGCCGTC CAGCTTTTCA AGGGGAAGTT CTTTTATTGT 
ACAGATGAGT CCAAGGAATT CCAGAGAGAC TGCAGGGGAG AATACCTGGT CTATGACAAA 
AGTGAAGTTA AAGCACAGAA ACGAGAGTGG AAGAAATGGC CGTTCCATTA TGACAATGTG 
CTATGGGCAC TTCTCACCCT CTTTACTGTA TCTACAGGGG AAGGTTGGCC AGATGTGCTC 
AGACATTCTG TGGATGCCAC CTATGAGGAT CAGGGTCCCA GGCCTGGTTT TCGGATGGAG 
ATGTCTATAT TTTATGTGGT CTACTTTGTG GTCTTCCCTT TCTTCTTTGT CAACATCTTT 
GTGGCCTTGA TTATCATAAC TTTCCAGGAG CAGGGTGACA AAATAATGGA GGAGTACAGT 
TTGGAAAAGA ATGAGAGAGC TTGCATAGAC TTTGCTATCA GTGCCAAGCC GCTGACGCGC 
CATATGCCGC AGAACAAGCA GAGCTTTCAG TATCGCATGT GGCAGTTTGT GGTGTCTCCC 
CCCTTTGAAT ACACCATCAT GGCTCTCATC GCACTCAATA CGATTGTGTT AATGATGAAG 
TTCCACGGAT CTTCTAATAC ATATGAAAAT GTGCTGAAAA CATTCAACAT GGTCTTCACC 
TCCCTCTTCT CTCTTGAATG CCTTCTCAAA ATAATGGCTT TCGGTGCTCC GAATTATTTC 
CGGGACGCCT GGAACATCTT TGATTTTGTG ACGGTTCTTG GGAGCATCAC GGATATTGTA 
GTGACTGAGC TCTGGGACAG CATCATCAAC CTCAGCTTCC TCCGTCTGTT TCGAGCTGCC 
CGACTTATTA AACTCCTGCG GCAGGGATAC ACCATTCGTA TTCTACTGTG GACCTTCGTC 
CAGTCCTTCA AGGCTCTACC CTACGTCTGC CTCCTGATCG CTATGCTGTT CTTTATTTAT 
GCCATCATCG GCATGCAGGT GTTCGGTAAC ATTGCCTTGG ATGAAGAAGA CGACGACACA 
GCTATAACCG AGCACAATAA TTTCCGCAAC TTTTTCCAAG CTCTTATGCT TCTCTTCCGC 
AGTGCCACGG GCGAGGCTTG GCATGAAATC ATGCTGTCCT GTTTGAGTGG GAGACCATGT 
GACGTCGGGT CCGGATTAAA GGAGAAGGAG TGCGGCAGTG AATTTGCTTA TTTTTACTTT 
GTGTCGTTCA TATTCCTCTG TTCCTTCCTG ATGTTAAACC TGTTTGTTGC TGTCATCATG 
GACAACTTTG AATATCTGAC CCGTGATTCA TCCATCTTGG GCCCTCATCA CCTTGATGAA 
TACGTGCGAG TATGGGCGGA GTATGATCCT GCCGCTTGCG GGCGTATTCA TTATAAGGAC 
ATGTACAGTT TGTTACGGGT TATCTCCCCT CCGCTGGGGT TGGGGAAGAA ATGCCCCCAC 
AGGGTGGCCT GCAAGAGGTT ACTCCGAATG GATCTTCCTG TTGCTGACGA TAACACTGTG 
CATTTCAACT CTACTCTGAT GGCTCTGATT AGAACTGCGT TGGACATCAA GATTGCCAAG 
GGTGGGGCAG ACAAGCAGCA GATGGACAGT CAACTCAGAA AGGAAATGAT GGCTATTTGG 
CCAAACATGT CCCAGAAAAC ACTGGACCTA TTGGTGACCC CACATAAGTC TACTGACCTC 
ACTGTTGGAA AGATCTATGC TGCCATGATG ATTATGGAGT ATTACAGGCA GAGCAAAGCA 
AAAAGGCTTC AGGCTCTGCA GGAGGAACAG AACCGCACCC CTCTCATGTT CCAGCGAATG 
GAACCACCAT CGCCCACACA GGATGGACCC TTAGGAATCA ATGCTTTGCC GTCCTCCCAA 
GCTGGAGAAA TGGGGCTGCC TGAAGGGGGC ATGAAGGAGA GCCAGTCTTG GATGACCCAA 
CGGGCCCAGC AGATGTTTCA GAAAACAGGG ACCTGGAGCC CAGAGAGGGC GCGCACACAG 
GAAAATACCA ACAGCTGGCC AAATTCCCAA CAGTCTGTAG AGATGAGTGA CATGGGTCGG 
GACGGTTACT CAGACAGCGA GCAGTACCTG GTCATGGAGG GACATGGAAG AGCCGCTTCA 
ATGCCACGGC TTCCAGCTGA TAACCAGCAG CGAAGGAAAA CCCGAACCAG AGGGAGTAAT 
CTCAGTACTA TTACGGACGG GAGTCCTATG AAACGTTCCG CCTCCATGCT CGGCCACTCC 
CAGTCTAAAG GCATCAGATT GGATGAGTAT TCATTAGAGA GGGTTACACC TGAGGAAGGA 
CCTCGCCATG GGCACCAACG CAGGAGGGAG AGGGGACACA GGGTCTCTGA CAGATCCCTC 
ACCCGATATG TGGATCTGGA GTCAGGATTG GGTACAGAGC TCAGCACTAC AACACAGTCA 
TGTGATCTCC CCACTGAAGA GCGTGGACGC ACTCGAGATC GGAAGCACCA CCATCACCAC 
CACCACCATC ATCATCACCA TCATCACCAC CCCAGCACTG AACGGGAGAG ATTAGAGTAC 
GGAAGGCCAA GCTCTCGCTC CCCCAGCACA GGGCGAGATT ATGCTCCACA CAGACAGGGC 
AGTAGTTCAG TGGATGGCAG CCCCGTGCCC TCAACCTCTG GGGCCAGCAC ACCCCGCAGA 
GGAAGGCGAC AGCTGCCCCA GCTGCCAACA ACTCCCAGGC CACGAGTTAC TTATTCTCCA 
GTGGTCCGCC GGCCTGCCAG CGTTTGCTCT CCCCAGCAGC AGTCCTTGTG CAGAATGACC 
ACCCCTATCC AACGGAGATA TCCCCAGACT GAGAACCGTC CAAGCAGCAA TGAAAGACGG 
TCCGTTAACA ATGATTCCCC CCGATCCCAT CGTCACACAA GAGGACACTG CACGGAAGTC 
CCCCGCGCTG GATGCAGGCA CAGCGGCCCT TATCGAGATT CCTCTTTTGA GGACCCTCAT 
CCCTGCGATC AGCGCTCCTC CAGGACTGCC GAGTCTGGGG GGGTACCCCC TCTCTCACCG 
CGCGGGCGCA GGTTGCCCAA TGGTTACTCT CATGGGACCC CGCGATCCAC AAAATCACGT 
CATGAACGTT ACAGTGAGAG TGAGGAGGAT GACTGGTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cacna1a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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