dgkd cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgkd
Entrez Gene ID	100492122
Full Name	diacylglycerol kinase delta
General protein information	Preferred Names diacylglycerol kinase delta Names diacylglycerol kinase delta diacylglycerol kinase, delta 130kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094445.1	XP_017949934.1	diacylglycerol kinase delta isoform X2
XM_031902541.1	XP_031758401.1	diacylglycerol kinase delta isoform X1
XM_004917864.4	XP_004917921.1	diacylglycerol kinase delta isoform X2
XM_004917864.3	XP_004917921.1	diacylglycerol kinase delta isoform X1
XM_031902542.1	XP_031758402.1	diacylglycerol kinase delta isoform X3
XM_004917865.3	XP_004917922.1	diacylglycerol kinase delta isoform X3
XM_012971151.2	XP_012826605.1	diacylglycerol kinase delta isoform X4
XM_031902543.1	XP_031758403.1	diacylglycerol kinase delta isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKD	NP_690618.2
Pan troglodytes (chimpanzee)	DGKD	XP_003309576.1
Gallus gallus (chicken)	DGKD	XP_004937002.1
Xenopus tropicalis (tropical clawed frog)	dgkd	XP_002936660.1
Rattus norvegicus (Norway rat)	Dgkd	XP_006245566.1
Mus musculus (house mouse)	Dgkd	NP_808314.2
	DGKD	XP_001114920.1
Canis lupus familiaris (dog)	DGKD	XP_005635924.1
Bos taurus (cattle)	DGKD	NP_001030359.2

Gene Ontology

The following dgkd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgkd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16156	XM_004917864.4 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa44792	XM_018094445.1 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa58863	XM_031902541.1 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58864	XM_031902542.1 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa58865	XM_031902543.1 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16158	XM_004917865.3 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa35278	XM_012971151.2 Latest version!	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa16156	XM_004917864.3	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgkd ( XM_004917864.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917921.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16156
Clone ID Related Accession (Same CDS sequence)	XM_004917864.4 , XM_004917864.3
Accession Version	XM_004917864.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917864.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGCTCATCTT 
TGGGGGACGG AAGAGGTGTC TCTGTGGCTG GAGCGGCTCA GCTTGTCTGA GTACAAGGAG 
GTCTTTACTC GCCATGACAT CAGAGGCTGT GAGCTCCTAC ATCTGGAGCG TCGAGACCTC 
AAGGAACTGG GTGTGACCAA AGTGGGCCAC ATGAAGAGAA TCCTTCACGG CATCCGGGAG 
CTGAGTCCGC CTCCTATCAA TGCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917921.1
CDS	191..3760
Translation	MAASLAAVAPGAPAEESSDSEPEPEPQKLRRKVSTSGQIRGKAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_004917864.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917864.4

ORF Insert Sequence:

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGCTCATCTT 
TGGGGGACGG AAGAGGTGTC TCTGTGGCTG GAGCGGCTCA GCTTGTCTGA GTACAAGGAG 
GTCTTTACTC GCCATGACAT CAGAGGCTGT GAGCTCCTAC ATCTGGAGCG TCGAGACCTC 
AAGGAACTGG GTGTGACCAA AGTGGGCCAC ATGAAGAGAA TCCTTCACGG CATCCGGGAG 
CTGAGTCCGC CTCCTATCAA TGCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_018094445.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949934.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa44792
Clone ID Related Accession (Same CDS sequence)	XM_018094445.1
Accession Version	XM_018094445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGGTA 
TTTACCCCCT TTCGGAAACT GATCCTCAGT GCAGACAACA GAAAGGACAT GGAGGAATGG 
ATGGCAGCAC TGAAGTCTGT GCAGAACCGT GAACTCTTTG AGTCAGCCCA GTACAGCATG 
GACCACTTCT CGGGAATGCA TAACTGGTAT GCTTGTTCCC ACGCGCGACC CACTTACTGT 
AATGTATGCC GTGAAGCCCT TTCTGGGGTT ACCTCTCATG GACTGTCATG TGAAGTGTGT 
AAATTTAAAG CTCACAAACG GTGTGCTGTA CGGGCCACCA ATAACTGTAA ATGGACTACC 
TTGGCATCTA TTGGCAAAGA CATTATAGAA GATGAAGATG GGGTATCAAT GCCGCACCAG 
TGGCTGGAAG GTAACCTACC TGTGAGTGCA AAATGCACAG TATGTGAAAA GACCTGTGGC 
AGTGTGCTCC GTTTACAGGA CTGGCAATGT CTGTGGTGCA AAGCAGCGGT ACACTCATTG 
TGTAAAGATC TCCTCCCTAA AAAGTGCCCT CTAGGCCAGT GCAAAGTTTC AGTCATTCCT 
CCCACGGCTC TCAACAGCAT TGACTCAGAT GGTTTCTGGA AGGCCAGCTG CCCACCATCC 
TGTACTAGCC CTTTACTTGT TTTTGTCAAT TCAAAAAGTG GGGACAACCA AGGGGTGAAG 
TTCTTACGGC GTTTCAAACA ACTTCTGAAC CCAGCTCAAG TTTTTGACCT CATGAACGGG 
GGGCCGCACC TTGGTTTACG ATTATTTCAA AAGTTTGACA CATTTCGAAT CCTGGTATGT 
GGTGGTGATG GAAGTGTTGG ATGGGTTCTC TCTGAAATAG ATACACTCAA TCTTCACAAA 
CAGTGCCAAC TTGGAGTGCT GCCCCTGGGA ACTGGGAATG ACCTTGCTCG TGTGCTTGGT 
TGGGGTTCTG CTTGTGATGA TGATACTCAA CTCCCACAAA TCCTAGAGAA GCTGGAGAGG 
GCAGGCACTA AGATGCTGGA TAGATGGAGT ATCATGGTGT ATGAGACTAA ATTGCCAGCT 
CAGTCCCCAG ATTCTTGCAC AGCTCCAGAA AACAATGAGG ATTGCGAGGT GCAACAAATC 
CTGTGTTATG AAGACTCCGT GGCAGCTCAT CTATCTCAGA TCCTCAGCTC AGACCAGCAC 
TCTGTGGTTA TCTCATCAGC CAAGGTTCTC TGTGAAACTG TGAAAGACTT TGTTGCTCGA 
GTTGGAAAAG CTTATGAGAG AATGACTGAG AGCTGCGAAG AATCTGAGGC CATGGCAGGA 
AAGTGTTCAC TTTTGAGAGA GAAATTGGAT TCACTCCTGA AGACCCTGAA TGAGGAGTCA 
CAAGCTACCT CAGTCATGTC TCAGCCCCCT CCTACCATTA ATGAGGAGGG GGAGGAAACG 
GAGTCCTGTT CACCAAATAT GTCTCCATCA CCAGCCCGCA CACAAAGTTT TCGCCCCCGG 
GAACAACTAA TGCTTAGAGC CAATAGCCTA AAGAAAGCTA TCCGACAAAT TATTGAGCAG 
GCAGAGAAAG CTGTAGATGA ACAGAATGTA CAGAATGCAC ACACTGAAGA TTTCCTACTG 
TCCTTTTCCT TTGATTCTGC AAAAGAGAAA AATTCATCAA GTTCCTCAAA CTTGCAAAGT 
GCAAATGCGC TTCCCAGGAT AAGGAGCTCA AATAGAGTTG CCAAGACTGA GCAAATGAGC 
AACAAAACCA GCCTGACTGC AGGAGCATCT GCATCTCTCC CAGCCCCTTC CTCACACAGA 
GATAATTTAC CAGTGCTGAA CTCCAAAATA CTCTACTCAA ATATGAGAGC TGGGATGTCT 
GGGTCTTTGC CTGGAAACTC TGTCATTAGC CGTCTGTTAA TTAGTGCTGA CCCCTTTGGT 
TCAGAACCAG ATAATGTGGA CTGTTACACA GAAAAGTGTG TGATGAACAA TTACTTTGGG 
ATTGGGCTGG ATGCAAAAAT TTCCTTGGAT TTTAACAACA AGAGAGATGA GCACCCAAAA 
AAGTGCAGGA GCCGCACAAA GAACATGATG TGGTATGGAG TGCTAGGAAC AAAGGAACTC 
CTCCAAAGGA CGTACAAGAA CCTGGAACAG AGAGTTTTAC TAGAGTGTGA TGGGGTTTCT 
ATTCCCCTGC CCAGCCTCCA AGGCATCGCT GTGCTGAACA TTCCAAGTTA CGCGGGGGGC 
ACTAACTTCT GGGGCGGCAG CAAGGAGAAT GATACTTTTG CTGCCCCTTC TTTTGATGAT 
AAAATTCTGG AAGTGGTTGC TGTGTTCGGC AGTATGCAGA TGGCTGTGTC CAGAGTCATA 
AAGCTACAGC ATCACAGAAT TGCACAGTGT CGCACAGTGA AGATTACAGT GCTTGGTGAT 
GAGGGAGTTC CTGTGCAAGT GGATGGAGAA GCCTGGATCC AGCCACCAGG GTTTATACGT 
ATAGTACATA AGAACAGAAC GCAGACACTC ACTAGAGACA GGGCTTTTGA AAGCACATTA 
AAGTCCTGGG AGGACAAGCA GAAATGTGAC ATTCCTCGCC CACCTGTATC GCCTGTGTCT 
CCCACAGCTT TGCAGCCTGA GGAGCTCTCT GAAGATGAGA CTGCTCAAGT ATCCCGGTTT 
GGCCACTCTG CCAGTGCACT TATTCAAAGC ATTAAGGAGA TAGCTGTTAC CCACCAGAGC 
ATGGAGCAGG AGCTAGCTCA TGCTGTCAAC GCCAGCTCAA AATCTATGGA AAAGGTTTAT 
AAGGACTCTG AGGGCCTGAG CTGTAGTCAT GTTATGGAGA TGGTTACAAA TGTTCGAGCA 
CTTCTTAATG AAACAGAACT ACTCCTTGCA GGGAAAATGG CATTGCTGCT GGATCCCCCT 
CAGAAGGAGC AGCTAGTGAC ATCCCTTAGC AACACAGAGC AGGAGCTGAG AAAACTGGGT 
GAAATCCCTC GACTGTGGCA AACTAATGAG GAAGAGGGTA CCCTAGACTT TTCCAAATTA 
AGAAGGAGTA GTCGGTTTCG ACTGGTAAAC AAGTTTAAAA AGGAAAAGAA TTCAAGGAAC 
AAGGAGACTC GAAGCAGTAC AGGGCCTCCA GCTCATCTTT GGGGGACGGA AGAGGTGTCT 
CTGTGGCTGG AGCGGCTCAG CTTGTCTGAG TACAAGGAGG TCTTTACTCG CCATGACATC 
AGAGGCTGTG AGCTCCTACA TCTGGAGCGT CGAGACCTCA AGGAACTGGG TGTGACCAAA 
GTGGGCCACA TGAAGAGAAT CCTTCACGGC ATCCGGGAGC TGAGTCCGCC TCCTATCAAT 
GCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949934.1
CDS	176..3664
Translation	MAASLAAVAPGAPAEESSDSEPEPEPQKLRRKVSTSGQIRGKAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_018094445.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094445.1

ORF Insert Sequence:

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGGTA 
TTTACCCCCT TTCGGAAACT GATCCTCAGT GCAGACAACA GAAAGGACAT GGAGGAATGG 
ATGGCAGCAC TGAAGTCTGT GCAGAACCGT GAACTCTTTG AGTCAGCCCA GTACAGCATG 
GACCACTTCT CGGGAATGCA TAACTGGTAT GCTTGTTCCC ACGCGCGACC CACTTACTGT 
AATGTATGCC GTGAAGCCCT TTCTGGGGTT ACCTCTCATG GACTGTCATG TGAAGTGTGT 
AAATTTAAAG CTCACAAACG GTGTGCTGTA CGGGCCACCA ATAACTGTAA ATGGACTACC 
TTGGCATCTA TTGGCAAAGA CATTATAGAA GATGAAGATG GGGTATCAAT GCCGCACCAG 
TGGCTGGAAG GTAACCTACC TGTGAGTGCA AAATGCACAG TATGTGAAAA GACCTGTGGC 
AGTGTGCTCC GTTTACAGGA CTGGCAATGT CTGTGGTGCA AAGCAGCGGT ACACTCATTG 
TGTAAAGATC TCCTCCCTAA AAAGTGCCCT CTAGGCCAGT GCAAAGTTTC AGTCATTCCT 
CCCACGGCTC TCAACAGCAT TGACTCAGAT GGTTTCTGGA AGGCCAGCTG CCCACCATCC 
TGTACTAGCC CTTTACTTGT TTTTGTCAAT TCAAAAAGTG GGGACAACCA AGGGGTGAAG 
TTCTTACGGC GTTTCAAACA ACTTCTGAAC CCAGCTCAAG TTTTTGACCT CATGAACGGG 
GGGCCGCACC TTGGTTTACG ATTATTTCAA AAGTTTGACA CATTTCGAAT CCTGGTATGT 
GGTGGTGATG GAAGTGTTGG ATGGGTTCTC TCTGAAATAG ATACACTCAA TCTTCACAAA 
CAGTGCCAAC TTGGAGTGCT GCCCCTGGGA ACTGGGAATG ACCTTGCTCG TGTGCTTGGT 
TGGGGTTCTG CTTGTGATGA TGATACTCAA CTCCCACAAA TCCTAGAGAA GCTGGAGAGG 
GCAGGCACTA AGATGCTGGA TAGATGGAGT ATCATGGTGT ATGAGACTAA ATTGCCAGCT 
CAGTCCCCAG ATTCTTGCAC AGCTCCAGAA AACAATGAGG ATTGCGAGGT GCAACAAATC 
CTGTGTTATG AAGACTCCGT GGCAGCTCAT CTATCTCAGA TCCTCAGCTC AGACCAGCAC 
TCTGTGGTTA TCTCATCAGC CAAGGTTCTC TGTGAAACTG TGAAAGACTT TGTTGCTCGA 
GTTGGAAAAG CTTATGAGAG AATGACTGAG AGCTGCGAAG AATCTGAGGC CATGGCAGGA 
AAGTGTTCAC TTTTGAGAGA GAAATTGGAT TCACTCCTGA AGACCCTGAA TGAGGAGTCA 
CAAGCTACCT CAGTCATGTC TCAGCCCCCT CCTACCATTA ATGAGGAGGG GGAGGAAACG 
GAGTCCTGTT CACCAAATAT GTCTCCATCA CCAGCCCGCA CACAAAGTTT TCGCCCCCGG 
GAACAACTAA TGCTTAGAGC CAATAGCCTA AAGAAAGCTA TCCGACAAAT TATTGAGCAG 
GCAGAGAAAG CTGTAGATGA ACAGAATGTA CAGAATGCAC ACACTGAAGA TTTCCTACTG 
TCCTTTTCCT TTGATTCTGC AAAAGAGAAA AATTCATCAA GTTCCTCAAA CTTGCAAAGT 
GCAAATGCGC TTCCCAGGAT AAGGAGCTCA AATAGAGTTG CCAAGACTGA GCAAATGAGC 
AACAAAACCA GCCTGACTGC AGGAGCATCT GCATCTCTCC CAGCCCCTTC CTCACACAGA 
GATAATTTAC CAGTGCTGAA CTCCAAAATA CTCTACTCAA ATATGAGAGC TGGGATGTCT 
GGGTCTTTGC CTGGAAACTC TGTCATTAGC CGTCTGTTAA TTAGTGCTGA CCCCTTTGGT 
TCAGAACCAG ATAATGTGGA CTGTTACACA GAAAAGTGTG TGATGAACAA TTACTTTGGG 
ATTGGGCTGG ATGCAAAAAT TTCCTTGGAT TTTAACAACA AGAGAGATGA GCACCCAAAA 
AAGTGCAGGA GCCGCACAAA GAACATGATG TGGTATGGAG TGCTAGGAAC AAAGGAACTC 
CTCCAAAGGA CGTACAAGAA CCTGGAACAG AGAGTTTTAC TAGAGTGTGA TGGGGTTTCT 
ATTCCCCTGC CCAGCCTCCA AGGCATCGCT GTGCTGAACA TTCCAAGTTA CGCGGGGGGC 
ACTAACTTCT GGGGCGGCAG CAAGGAGAAT GATACTTTTG CTGCCCCTTC TTTTGATGAT 
AAAATTCTGG AAGTGGTTGC TGTGTTCGGC AGTATGCAGA TGGCTGTGTC CAGAGTCATA 
AAGCTACAGC ATCACAGAAT TGCACAGTGT CGCACAGTGA AGATTACAGT GCTTGGTGAT 
GAGGGAGTTC CTGTGCAAGT GGATGGAGAA GCCTGGATCC AGCCACCAGG GTTTATACGT 
ATAGTACATA AGAACAGAAC GCAGACACTC ACTAGAGACA GGGCTTTTGA AAGCACATTA 
AAGTCCTGGG AGGACAAGCA GAAATGTGAC ATTCCTCGCC CACCTGTATC GCCTGTGTCT 
CCCACAGCTT TGCAGCCTGA GGAGCTCTCT GAAGATGAGA CTGCTCAAGT ATCCCGGTTT 
GGCCACTCTG CCAGTGCACT TATTCAAAGC ATTAAGGAGA TAGCTGTTAC CCACCAGAGC 
ATGGAGCAGG AGCTAGCTCA TGCTGTCAAC GCCAGCTCAA AATCTATGGA AAAGGTTTAT 
AAGGACTCTG AGGGCCTGAG CTGTAGTCAT GTTATGGAGA TGGTTACAAA TGTTCGAGCA 
CTTCTTAATG AAACAGAACT ACTCCTTGCA GGGAAAATGG CATTGCTGCT GGATCCCCCT 
CAGAAGGAGC AGCTAGTGAC ATCCCTTAGC AACACAGAGC AGGAGCTGAG AAAACTGGGT 
GAAATCCCTC GACTGTGGCA AACTAATGAG GAAGAGGGTA CCCTAGACTT TTCCAAATTA 
AGAAGGAGTA GTCGGTTTCG ACTGGTAAAC AAGTTTAAAA AGGAAAAGAA TTCAAGGAAC 
AAGGAGACTC GAAGCAGTAC AGGGCCTCCA GCTCATCTTT GGGGGACGGA AGAGGTGTCT 
CTGTGGCTGG AGCGGCTCAG CTTGTCTGAG TACAAGGAGG TCTTTACTCG CCATGACATC 
AGAGGCTGTG AGCTCCTACA TCTGGAGCGT CGAGACCTCA AGGAACTGGG TGTGACCAAA 
GTGGGCCACA TGAAGAGAAT CCTTCACGGC ATCCGGGAGC TGAGTCCGCC TCCTATCAAT 
GCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_031902541.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758401.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.

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CloneID	OXa58863
Clone ID Related Accession (Same CDS sequence)	XM_031902541.1
Accession Version	XM_031902541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGTTGCC 
AAGACTGAGC AAATGAGCAA CAAAACCAGC CTGACTGCAG GAGCATCTGC ATCTCTCCCA 
GCCCCTTCCT CACACAGAGA TAATTTACCA GTGCTGAACT CCAAAATACT CTACTCAAAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGC TCATCTTTGG 
GGGACGGAAG AGGTGTCTCT GTGGCTGGAG CGGCTCAGCT TGTCTGAGTA CAAGGAGGTC 
TTTACTCGCC ATGACATCAG AGGCTGTGAG CTCCTACATC TGGAGCGTCG AGACCTCAAG 
GAACTGGGTG TGACCAAAGT GGGCCACATG AAGAGAATCC TTCACGGCAT CCGGGAGCTG 
AGTCCGCCTC CTATCAATGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758401.1
CDS	16..3522
Translation	MFMADRTSILRADFGTGETGLAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_031902541.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902541.1

ORF Insert Sequence:

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGTTGCC 
AAGACTGAGC AAATGAGCAA CAAAACCAGC CTGACTGCAG GAGCATCTGC ATCTCTCCCA 
GCCCCTTCCT CACACAGAGA TAATTTACCA GTGCTGAACT CCAAAATACT CTACTCAAAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGC TCATCTTTGG 
GGGACGGAAG AGGTGTCTCT GTGGCTGGAG CGGCTCAGCT TGTCTGAGTA CAAGGAGGTC 
TTTACTCGCC ATGACATCAG AGGCTGTGAG CTCCTACATC TGGAGCGTCG AGACCTCAAG 
GAACTGGGTG TGACCAAAGT GGGCCACATG AAGAGAATCC TTCACGGCAT CCGGGAGCTG 
AGTCCGCCTC CTATCAATGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_031902542.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758402.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58864
Clone ID Related Accession (Same CDS sequence)	XM_031902542.1
Accession Version	XM_031902542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGTTGCC 
AAGACTGAGC AAATGAGCAA CAAAACCAGC CTGACTGCAG GAGCATCTGC ATCTCTCCCA 
GCCCCTTCCT CACACAGAGA TAATTTACCA GTGCTGAACT CCAAAATACT CTACTCAAAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGG AACTGGGTGT 
GACCAAAGTG GGCCACATGA AGAGAATCCT TCACGGCATC CGGGAGCTGA GTCCGCCTCC 
TATCAATGCG GATTAGGATC TGTCTTCACT TCATGTATCA CCCAGTCTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758402.1
CDS	16..3429
Translation	MFMADRTSILRADFGTGETGLAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPGTGCDQSGPHEENPSRHPGAESASYQCGLGSVFTSCITQSG

Target ORF information:

RefSeq Version	XM_031902542.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902542.1

ORF Insert Sequence:

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGTTGCC 
AAGACTGAGC AAATGAGCAA CAAAACCAGC CTGACTGCAG GAGCATCTGC ATCTCTCCCA 
GCCCCTTCCT CACACAGAGA TAATTTACCA GTGCTGAACT CCAAAATACT CTACTCAAAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGG AACTGGGTGT 
GACCAAAGTG GGCCACATGA AGAGAATCCT TCACGGCATC CGGGAGCTGA GTCCGCCTCC 
TATCAATGCG GATTAGGATC TGTCTTCACT TCATGTATCA CCCAGTCTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_031902543.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758403.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa58865
Clone ID Related Accession (Same CDS sequence)	XM_031902543.1
Accession Version	XM_031902543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGATATG 
AGAGCTGGGA TGTCTGGGTC TTTGCCTGGA AACTCTGTCA TTAGCCGTCT GTTAATTAGT 
GCTGACCCCT TTGGTTCAGA ACCAGATAAT GTGGACTGTT ACACAGAAAA GTGTGTGATG 
AACAATTACT TTGGGATTGG GCTGGATGCA AAAATTTCCT TGGATTTTAA CAACAAGAGA 
GATGAGCACC CAAAAAAGTG CAGGAGCCGC ACAAAGAACA TGATGTGGTA TGGAGTGCTA 
GGAACAAAGG AACTCCTCCA AAGGACGTAC AAGAACCTGG AACAGAGAGT TTTACTAGAG 
TGTGATGGGG TTTCTATTCC CCTGCCCAGC CTCCAAGGCA TCGCTGTGCT GAACATTCCA 
AGTTACGCGG GGGGCACTAA CTTCTGGGGC GGCAGCAAGG AGAATGATAC TTTTGCTGCC 
CCTTCTTTTG ATGATAAAAT TCTGGAAGTG GTTGCTGTGT TCGGCAGTAT GCAGATGGCT 
GTGTCCAGAG TCATAAAGCT ACAGCATCAC AGAATTGCAC AGTGTCGCAC AGTGAAGATT 
ACAGTGCTTG GTGATGAGGG AGTTCCTGTG CAAGTGGATG GAGAAGCCTG GATCCAGCCA 
CCAGGGTTTA TACGTATAGT ACATAAGAAC AGAACGCAGA CACTCACTAG AGACAGGGCT 
TTTGAAAGCA CATTAAAGTC CTGGGAGGAC AAGCAGAAAT GTGACATTCC TCGCCCACCT 
GTATCGCCTG TGTCTCCCAC AGCTTTGCAG CCTGAGGAGC TCTCTGAAGA TGAGACTGCT 
CAAGTATCCC GGTTTGGCCA CTCTGCCAGT GCACTTATTC AAAGCATTAA GGAGATAGCT 
GTTACCCACC AGAGCATGGA GCAGGAGCTA GCTCATGCTG TCAACGCCAG CTCAAAATCT 
ATGGAAAAGG TTTATAAGGA CTCTGAGGGC CTGAGCTGTA GTCATGTTAT GGAGATGGTT 
ACAAATGTTC GAGCACTTCT TAATGAAACA GAACTACTCC TTGCAGGGAA AATGGCATTG 
CTGCTGGATC CCCCTCAGAA GGAGCAGCTA GTGACATCCC TTAGCAACAC AGAGCAGGAG 
CTGAGAAAAC TGGGTGAAAT CCCTCGACTG TGGCAAACTA ATGAGGAAGA GGGTACCCTA 
GACTTTTCCA AATTAAGAAG GAGTAGTCGG TTTCGACTGG TAAACAAGTT TAAAAAGGAA 
AAGAATTCAA GGAACAAGGA GACTCGAAGC AGTACAGGGC CTCCAGCTCA TCTTTGGGGG 
ACGGAAGAGG TGTCTCTGTG GCTGGAGCGG CTCAGCTTGT CTGAGTACAA GGAGGTCTTT 
ACTCGCCATG ACATCAGAGG CTGTGAGCTC CTACATCTGG AGCGTCGAGA CCTCAAGGAA 
CTGGGTGTGA CCAAAGTGGG CCACATGAAG AGAATCCTTC ACGGCATCCG GGAGCTGAGT 
CCGCCTCCTA TCAATGCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758403.1
CDS	16..3399
Translation	MFMADRTSILRADFGTGETGLAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRDMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_031902543.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902543.1

ORF Insert Sequence:

ATGTTTATGG CTGATAGAAC ATCGATTCTG AGGGCTGATT TTGGCACTGG AGAAACTGGC 
CTGGCTATCA TAAAGGAGGG GCACCTGCAT AAGCAAACCA GTTCCTTTCA GAGATGGAAA 
CGGCGCTATT TCAAACTCAG AGGGAGAACT CTTTACTATG CCAAGACTGC CAAGTCAATC 
ATATTTGAAG AAGTTGATAT AACTGATGCA AGTGTTGCTG AATCCAGTAC CAAGAATCTT 
AATAACAGCT TCACCGTATT TACCCCCTTT CGGAAACTGA TCCTCAGTGC AGACAACAGA 
AAGGACATGG AGGAATGGAT GGCAGCACTG AAGTCTGTGC AGAACCGTGA ACTCTTTGAG 
TCAGCCCAGT ACAGCATGGA CCACTTCTCG GGAATGCATA ACTGGTATGC TTGTTCCCAC 
GCGCGACCCA CTTACTGTAA TGTATGCCGT GAAGCCCTTT CTGGGGTTAC CTCTCATGGA 
CTGTCATGTG AAGTGTGTAA ATTTAAAGCT CACAAACGGT GTGCTGTACG GGCCACCAAT 
AACTGTAAAT GGACTACCTT GGCATCTATT GGCAAAGACA TTATAGAAGA TGAAGATGGG 
GTATCAATGC CGCACCAGTG GCTGGAAGGT AACCTACCTG TGAGTGCAAA ATGCACAGTA 
TGTGAAAAGA CCTGTGGCAG TGTGCTCCGT TTACAGGACT GGCAATGTCT GTGGTGCAAA 
GCAGCGGTAC ACTCATTGTG TAAAGATCTC CTCCCTAAAA AGTGCCCTCT AGGCCAGTGC 
AAAGTTTCAG TCATTCCTCC CACGGCTCTC AACAGCATTG ACTCAGATGG TTTCTGGAAG 
GCCAGCTGCC CACCATCCTG TACTAGCCCT TTACTTGTTT TTGTCAATTC AAAAAGTGGG 
GACAACCAAG GGGTGAAGTT CTTACGGCGT TTCAAACAAC TTCTGAACCC AGCTCAAGTT 
TTTGACCTCA TGAACGGGGG GCCGCACCTT GGTTTACGAT TATTTCAAAA GTTTGACACA 
TTTCGAATCC TGGTATGTGG TGGTGATGGA AGTGTTGGAT GGGTTCTCTC TGAAATAGAT 
ACACTCAATC TTCACAAACA GTGCCAACTT GGAGTGCTGC CCCTGGGAAC TGGGAATGAC 
CTTGCTCGTG TGCTTGGTTG GGGTTCTGCT TGTGATGATG ATACTCAACT CCCACAAATC 
CTAGAGAAGC TGGAGAGGGC AGGCACTAAG ATGCTGGATA GATGGAGTAT CATGGTGTAT 
GAGACTAAAT TGCCAGCTCA GTCCCCAGAT TCTTGCACAG CTCCAGAAAA CAATGAGGAT 
TGCGAGGTGC AACAAATCCT GTGTTATGAA GACTCCGTGG CAGCTCATCT ATCTCAGATC 
CTCAGCTCAG ACCAGCACTC TGTGGTTATC TCATCAGCCA AGGTTCTCTG TGAAACTGTG 
AAAGACTTTG TTGCTCGAGT TGGAAAAGCT TATGAGAGAA TGACTGAGAG CTGCGAAGAA 
TCTGAGGCCA TGGCAGGAAA GTGTTCACTT TTGAGAGAGA AATTGGATTC ACTCCTGAAG 
ACCCTGAATG AGGAGTCACA AGCTACCTCA GTCATGTCTC AGCCCCCTCC TACCATTAAT 
GAGGAGGGGG AGGAAACGGA GTCCTGTTCA CCAAATATGT CTCCATCACC AGCCCGCACA 
CAAAGTTTTC GCCCCCGGGA ACAACTAATG CTTAGAGCCA ATAGCCTAAA GAAAGCTATC 
CGACAAATTA TTGAGCAGGC AGAGAAAGCT GTAGATGAAC AGAATGTACA GAATGCACAC 
ACTGAAGATT TCCTACTGTC CTTTTCCTTT GATTCTGCAA AAGAGAAAAA TTCATCAAGT 
TCCTCAAACT TGCAAAGTGC AAATGCGCTT CCCAGGATAA GGAGCTCAAA TAGAGATATG 
AGAGCTGGGA TGTCTGGGTC TTTGCCTGGA AACTCTGTCA TTAGCCGTCT GTTAATTAGT 
GCTGACCCCT TTGGTTCAGA ACCAGATAAT GTGGACTGTT ACACAGAAAA GTGTGTGATG 
AACAATTACT TTGGGATTGG GCTGGATGCA AAAATTTCCT TGGATTTTAA CAACAAGAGA 
GATGAGCACC CAAAAAAGTG CAGGAGCCGC ACAAAGAACA TGATGTGGTA TGGAGTGCTA 
GGAACAAAGG AACTCCTCCA AAGGACGTAC AAGAACCTGG AACAGAGAGT TTTACTAGAG 
TGTGATGGGG TTTCTATTCC CCTGCCCAGC CTCCAAGGCA TCGCTGTGCT GAACATTCCA 
AGTTACGCGG GGGGCACTAA CTTCTGGGGC GGCAGCAAGG AGAATGATAC TTTTGCTGCC 
CCTTCTTTTG ATGATAAAAT TCTGGAAGTG GTTGCTGTGT TCGGCAGTAT GCAGATGGCT 
GTGTCCAGAG TCATAAAGCT ACAGCATCAC AGAATTGCAC AGTGTCGCAC AGTGAAGATT 
ACAGTGCTTG GTGATGAGGG AGTTCCTGTG CAAGTGGATG GAGAAGCCTG GATCCAGCCA 
CCAGGGTTTA TACGTATAGT ACATAAGAAC AGAACGCAGA CACTCACTAG AGACAGGGCT 
TTTGAAAGCA CATTAAAGTC CTGGGAGGAC AAGCAGAAAT GTGACATTCC TCGCCCACCT 
GTATCGCCTG TGTCTCCCAC AGCTTTGCAG CCTGAGGAGC TCTCTGAAGA TGAGACTGCT 
CAAGTATCCC GGTTTGGCCA CTCTGCCAGT GCACTTATTC AAAGCATTAA GGAGATAGCT 
GTTACCCACC AGAGCATGGA GCAGGAGCTA GCTCATGCTG TCAACGCCAG CTCAAAATCT 
ATGGAAAAGG TTTATAAGGA CTCTGAGGGC CTGAGCTGTA GTCATGTTAT GGAGATGGTT 
ACAAATGTTC GAGCACTTCT TAATGAAACA GAACTACTCC TTGCAGGGAA AATGGCATTG 
CTGCTGGATC CCCCTCAGAA GGAGCAGCTA GTGACATCCC TTAGCAACAC AGAGCAGGAG 
CTGAGAAAAC TGGGTGAAAT CCCTCGACTG TGGCAAACTA ATGAGGAAGA GGGTACCCTA 
GACTTTTCCA AATTAAGAAG GAGTAGTCGG TTTCGACTGG TAAACAAGTT TAAAAAGGAA 
AAGAATTCAA GGAACAAGGA GACTCGAAGC AGTACAGGGC CTCCAGCTCA TCTTTGGGGG 
ACGGAAGAGG TGTCTCTGTG GCTGGAGCGG CTCAGCTTGT CTGAGTACAA GGAGGTCTTT 
ACTCGCCATG ACATCAGAGG CTGTGAGCTC CTACATCTGG AGCGTCGAGA CCTCAAGGAA 
CTGGGTGTGA CCAAAGTGGG CCACATGAAG AGAATCCTTC ACGGCATCCG GGAGCTGAGT 
CCGCCTCCTA TCAATGCGGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_004917864.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917921.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.

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CloneID	OXa16156
Clone ID Related Accession (Same CDS sequence)	XM_004917864.4 , XM_004917864.3
Accession Version	XM_004917864.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGCTCATCTT 
TGGGGGACGG AAGAGGTGTC TCTGTGGCTG GAGCGGCTCA GCTTGTCTGA GTACAAGGAG 
GTCTTTACTC GCCATGACAT CAGAGGCTGT GAGCTCCTAC ATCTGGAGCG TCGAGACCTC 
AAGGAACTGG GTGTGACCAA AGTGGGCCAC ATGAAGAGAA TCCTTCACGG CATCCGGGAG 
CTGAGTCCGC CTCCTATCAA TGCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917921.1
CDS	177..3746
Translation	MAASLAAVAPGAPAEESSDSEPEPEPQKLRRKVSTSGQIRGKAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_004917864.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917864.3

ORF Insert Sequence:

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGCTCATCTT 
TGGGGGACGG AAGAGGTGTC TCTGTGGCTG GAGCGGCTCA GCTTGTCTGA GTACAAGGAG 
GTCTTTACTC GCCATGACAT CAGAGGCTGT GAGCTCCTAC ATCTGGAGCG TCGAGACCTC 
AAGGAACTGG GTGTGACCAA AGTGGGCCAC ATGAAGAGAA TCCTTCACGG CATCCGGGAG 
CTGAGTCCGC CTCCTATCAA TGCGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_004917865.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917922.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16158
Clone ID Related Accession (Same CDS sequence)	XM_004917865.3
Accession Version	XM_004917865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGGAACTGGG 
TGTGACCAAA GTGGGCCACA TGAAGAGAAT CCTTCACGGC ATCCGGGAGC TGAGTCCGCC 
TCCTATCAAT GCGGATTAGG ATCTGTCTTC ACTTCATGTA TCACCCAGTC TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917922.1
CDS	177..3653
Translation	MAASLAAVAPGAPAEESSDSEPEPEPQKLRRKVSTSGQIRGKAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRVAKTEQMSNKTSLTAGASASLPAPSSHRDNLPVLNSKILYSNMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPGTGCDQSGPHEENPSRHPGAESASYQCGLGSVFTSCITQSG

Target ORF information:

RefSeq Version	XM_004917865.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917865.3

ORF Insert Sequence:

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGTT 
GCCAAGACTG AGCAAATGAG CAACAAAACC AGCCTGACTG CAGGAGCATC TGCATCTCTC 
CCAGCCCCTT CCTCACACAG AGATAATTTA CCAGTGCTGA ACTCCAAAAT ACTCTACTCA 
AATATGAGAG CTGGGATGTC TGGGTCTTTG CCTGGAAACT CTGTCATTAG CCGTCTGTTA 
ATTAGTGCTG ACCCCTTTGG TTCAGAACCA GATAATGTGG ACTGTTACAC AGAAAAGTGT 
GTGATGAACA ATTACTTTGG GATTGGGCTG GATGCAAAAA TTTCCTTGGA TTTTAACAAC 
AAGAGAGATG AGCACCCAAA AAAGTGCAGG AGCCGCACAA AGAACATGAT GTGGTATGGA 
GTGCTAGGAA CAAAGGAACT CCTCCAAAGG ACGTACAAGA ACCTGGAACA GAGAGTTTTA 
CTAGAGTGTG ATGGGGTTTC TATTCCCCTG CCCAGCCTCC AAGGCATCGC TGTGCTGAAC 
ATTCCAAGTT ACGCGGGGGG CACTAACTTC TGGGGCGGCA GCAAGGAGAA TGATACTTTT 
GCTGCCCCTT CTTTTGATGA TAAAATTCTG GAAGTGGTTG CTGTGTTCGG CAGTATGCAG 
ATGGCTGTGT CCAGAGTCAT AAAGCTACAG CATCACAGAA TTGCACAGTG TCGCACAGTG 
AAGATTACAG TGCTTGGTGA TGAGGGAGTT CCTGTGCAAG TGGATGGAGA AGCCTGGATC 
CAGCCACCAG GGTTTATACG TATAGTACAT AAGAACAGAA CGCAGACACT CACTAGAGAC 
AGGGCTTTTG AAAGCACATT AAAGTCCTGG GAGGACAAGC AGAAATGTGA CATTCCTCGC 
CCACCTGTAT CGCCTGTGTC TCCCACAGCT TTGCAGCCTG AGGAGCTCTC TGAAGATGAG 
ACTGCTCAAG TATCCCGGTT TGGCCACTCT GCCAGTGCAC TTATTCAAAG CATTAAGGAG 
ATAGCTGTTA CCCACCAGAG CATGGAGCAG GAGCTAGCTC ATGCTGTCAA CGCCAGCTCA 
AAATCTATGG AAAAGGTTTA TAAGGACTCT GAGGGCCTGA GCTGTAGTCA TGTTATGGAG 
ATGGTTACAA ATGTTCGAGC ACTTCTTAAT GAAACAGAAC TACTCCTTGC AGGGAAAATG 
GCATTGCTGC TGGATCCCCC TCAGAAGGAG CAGCTAGTGA CATCCCTTAG CAACACAGAG 
CAGGAGCTGA GAAAACTGGG TGAAATCCCT CGACTGTGGC AAACTAATGA GGAAGAGGGT 
ACCCTAGACT TTTCCAAATT AAGAAGGAGT AGTCGGTTTC GACTGGTAAA CAAGTTTAAA 
AAGGAAAAGA ATTCAAGGAA CAAGGAGACT CGAAGCAGTA CAGGGCCTCC AGGAACTGGG 
TGTGACCAAA GTGGGCCACA TGAAGAGAAT CCTTCACGGC ATCCGGGAGC TGAGTCCGCC 
TCCTATCAAT GCGGATTAGG ATCTGTCTTC ACTTCATGTA TCACCCAGTC TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkd ( XM_012971151.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826605.1 Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.

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CloneID	OXa35278
Clone ID Related Accession (Same CDS sequence)	XM_012971151.2
Accession Version	XM_012971151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase delta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGC TCATCTTTGG 
GGGACGGAAG AGGTGTCTCT GTGGCTGGAG CGGCTCAGCT TGTCTGAGTA CAAGGAGGTC 
TTTACTCGCC ATGACATCAG AGGCTGTGAG CTCCTACATC TGGAGCGTCG AGACCTCAAG 
GAACTGGGTG TGACCAAAGT GGGCCACATG AAGAGAATCC TTCACGGCAT CCGGGAGCTG 
AGTCCGCCTC CTATCAATGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826605.1
CDS	176..3622
Translation	MAASLAAVAPGAPAEESSDSEPEPEPQKLRRKVSTSGQIRGKAIIKEGHLHKQTSSFQRWKRRYFKLRGRTLYYAKTAKSIIFEEVDITDASVAESSTKNLNNSFTVFTPFRKLILSADNRKDMEEWMAALKSVQNRELFESAQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVSMPHQWLEGNLPVSAKCTVCEKTCGSVLRLQDWQCLWCKAAVHSLCKDLLPKKCPLGQCKVSVIPPTALNSIDSDGFWKASCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDTLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERAGTKMLDRWSIMVYETKLPAQSPDSCTAPENNEDCEVQQILCYEDSVAAHLSQILSSDQHSVVISSAKVLCETVKDFVARVGKAYERMTESCEESEAMAGKCSLLREKLDSLLKTLNEESQATSVMSQPPPTINEEGEETESCSPNMSPSPARTQSFRPREQLMLRANSLKKAIRQIIEQAEKAVDEQNVQNAHTEDFLLSFSFDSAKEKNSSSSSNLQSANALPRIRSSNRDMRAGMSGSLPGNSVISRLLISADPFGSEPDNVDCYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPKKCRSRTKNMMWYGVLGTKELLQRTYKNLEQRVLLECDGVSIPLPSLQGIAVLNIPSYAGGTNFWGGSKENDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKITVLGDEGVPVQVDGEAWIQPPGFIRIVHKNRTQTLTRDRAFESTLKSWEDKQKCDIPRPPVSPVSPTALQPEELSEDETAQVSRFGHSASALIQSIKEIAVTHQSMEQELAHAVNASSKSMEKVYKDSEGLSCSHVMEMVTNVRALLNETELLLAGKMALLLDPPQKEQLVTSLSNTEQELRKLGEIPRLWQTNEEEGTLDFSKLRRSSRFRLVNKFKKEKNSRNKETRSSTGPPAHLWGTEEVSLWLERLSLSEYKEVFTRHDIRGCELLHLERRDLKELGVTKVGHMKRILHGIRELSPPPINAD

Target ORF information:

RefSeq Version	XM_012971151.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase delta (dgkd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971151.2

ORF Insert Sequence:

ATGGCGGCCT CTTTGGCTGC GGTAGCTCCA GGAGCCCCAG CTGAGGAATC ATCGGATAGC 
GAACCGGAAC CCGAGCCGCA GAAACTTCGA AGGAAAGTGT CCACGTCGGG ACAGATCCGG 
GGGAAGGCTA TCATAAAGGA GGGGCACCTG CATAAGCAAA CCAGTTCCTT TCAGAGATGG 
AAACGGCGCT ATTTCAAACT CAGAGGGAGA ACTCTTTACT ATGCCAAGAC TGCCAAGTCA 
ATCATATTTG AAGAAGTTGA TATAACTGAT GCAAGTGTTG CTGAATCCAG TACCAAGAAT 
CTTAATAACA GCTTCACCGT ATTTACCCCC TTTCGGAAAC TGATCCTCAG TGCAGACAAC 
AGAAAGGACA TGGAGGAATG GATGGCAGCA CTGAAGTCTG TGCAGAACCG TGAACTCTTT 
GAGTCAGCCC AGTACAGCAT GGACCACTTC TCGGGAATGC ATAACTGGTA TGCTTGTTCC 
CACGCGCGAC CCACTTACTG TAATGTATGC CGTGAAGCCC TTTCTGGGGT TACCTCTCAT 
GGACTGTCAT GTGAAGTGTG TAAATTTAAA GCTCACAAAC GGTGTGCTGT ACGGGCCACC 
AATAACTGTA AATGGACTAC CTTGGCATCT ATTGGCAAAG ACATTATAGA AGATGAAGAT 
GGGGTATCAA TGCCGCACCA GTGGCTGGAA GGTAACCTAC CTGTGAGTGC AAAATGCACA 
GTATGTGAAA AGACCTGTGG CAGTGTGCTC CGTTTACAGG ACTGGCAATG TCTGTGGTGC 
AAAGCAGCGG TACACTCATT GTGTAAAGAT CTCCTCCCTA AAAAGTGCCC TCTAGGCCAG 
TGCAAAGTTT CAGTCATTCC TCCCACGGCT CTCAACAGCA TTGACTCAGA TGGTTTCTGG 
AAGGCCAGCT GCCCACCATC CTGTACTAGC CCTTTACTTG TTTTTGTCAA TTCAAAAAGT 
GGGGACAACC AAGGGGTGAA GTTCTTACGG CGTTTCAAAC AACTTCTGAA CCCAGCTCAA 
GTTTTTGACC TCATGAACGG GGGGCCGCAC CTTGGTTTAC GATTATTTCA AAAGTTTGAC 
ACATTTCGAA TCCTGGTATG TGGTGGTGAT GGAAGTGTTG GATGGGTTCT CTCTGAAATA 
GATACACTCA ATCTTCACAA ACAGTGCCAA CTTGGAGTGC TGCCCCTGGG AACTGGGAAT 
GACCTTGCTC GTGTGCTTGG TTGGGGTTCT GCTTGTGATG ATGATACTCA ACTCCCACAA 
ATCCTAGAGA AGCTGGAGAG GGCAGGCACT AAGATGCTGG ATAGATGGAG TATCATGGTG 
TATGAGACTA AATTGCCAGC TCAGTCCCCA GATTCTTGCA CAGCTCCAGA AAACAATGAG 
GATTGCGAGG TGCAACAAAT CCTGTGTTAT GAAGACTCCG TGGCAGCTCA TCTATCTCAG 
ATCCTCAGCT CAGACCAGCA CTCTGTGGTT ATCTCATCAG CCAAGGTTCT CTGTGAAACT 
GTGAAAGACT TTGTTGCTCG AGTTGGAAAA GCTTATGAGA GAATGACTGA GAGCTGCGAA 
GAATCTGAGG CCATGGCAGG AAAGTGTTCA CTTTTGAGAG AGAAATTGGA TTCACTCCTG 
AAGACCCTGA ATGAGGAGTC ACAAGCTACC TCAGTCATGT CTCAGCCCCC TCCTACCATT 
AATGAGGAGG GGGAGGAAAC GGAGTCCTGT TCACCAAATA TGTCTCCATC ACCAGCCCGC 
ACACAAAGTT TTCGCCCCCG GGAACAACTA ATGCTTAGAG CCAATAGCCT AAAGAAAGCT 
ATCCGACAAA TTATTGAGCA GGCAGAGAAA GCTGTAGATG AACAGAATGT ACAGAATGCA 
CACACTGAAG ATTTCCTACT GTCCTTTTCC TTTGATTCTG CAAAAGAGAA AAATTCATCA 
AGTTCCTCAA ACTTGCAAAG TGCAAATGCG CTTCCCAGGA TAAGGAGCTC AAATAGAGAT 
ATGAGAGCTG GGATGTCTGG GTCTTTGCCT GGAAACTCTG TCATTAGCCG TCTGTTAATT 
AGTGCTGACC CCTTTGGTTC AGAACCAGAT AATGTGGACT GTTACACAGA AAAGTGTGTG 
ATGAACAATT ACTTTGGGAT TGGGCTGGAT GCAAAAATTT CCTTGGATTT TAACAACAAG 
AGAGATGAGC ACCCAAAAAA GTGCAGGAGC CGCACAAAGA ACATGATGTG GTATGGAGTG 
CTAGGAACAA AGGAACTCCT CCAAAGGACG TACAAGAACC TGGAACAGAG AGTTTTACTA 
GAGTGTGATG GGGTTTCTAT TCCCCTGCCC AGCCTCCAAG GCATCGCTGT GCTGAACATT 
CCAAGTTACG CGGGGGGCAC TAACTTCTGG GGCGGCAGCA AGGAGAATGA TACTTTTGCT 
GCCCCTTCTT TTGATGATAA AATTCTGGAA GTGGTTGCTG TGTTCGGCAG TATGCAGATG 
GCTGTGTCCA GAGTCATAAA GCTACAGCAT CACAGAATTG CACAGTGTCG CACAGTGAAG 
ATTACAGTGC TTGGTGATGA GGGAGTTCCT GTGCAAGTGG ATGGAGAAGC CTGGATCCAG 
CCACCAGGGT TTATACGTAT AGTACATAAG AACAGAACGC AGACACTCAC TAGAGACAGG 
GCTTTTGAAA GCACATTAAA GTCCTGGGAG GACAAGCAGA AATGTGACAT TCCTCGCCCA 
CCTGTATCGC CTGTGTCTCC CACAGCTTTG CAGCCTGAGG AGCTCTCTGA AGATGAGACT 
GCTCAAGTAT CCCGGTTTGG CCACTCTGCC AGTGCACTTA TTCAAAGCAT TAAGGAGATA 
GCTGTTACCC ACCAGAGCAT GGAGCAGGAG CTAGCTCATG CTGTCAACGC CAGCTCAAAA 
TCTATGGAAA AGGTTTATAA GGACTCTGAG GGCCTGAGCT GTAGTCATGT TATGGAGATG 
GTTACAAATG TTCGAGCACT TCTTAATGAA ACAGAACTAC TCCTTGCAGG GAAAATGGCA 
TTGCTGCTGG ATCCCCCTCA GAAGGAGCAG CTAGTGACAT CCCTTAGCAA CACAGAGCAG 
GAGCTGAGAA AACTGGGTGA AATCCCTCGA CTGTGGCAAA CTAATGAGGA AGAGGGTACC 
CTAGACTTTT CCAAATTAAG AAGGAGTAGT CGGTTTCGAC TGGTAAACAA GTTTAAAAAG 
GAAAAGAATT CAAGGAACAA GGAGACTCGA AGCAGTACAG GGCCTCCAGC TCATCTTTGG 
GGGACGGAAG AGGTGTCTCT GTGGCTGGAG CGGCTCAGCT TGTCTGAGTA CAAGGAGGTC 
TTTACTCGCC ATGACATCAG AGGCTGTGAG CTCCTACATC TGGAGCGTCG AGACCTCAAG 
GAACTGGGTG TGACCAAAGT GGGCCACATG AAGAGAATCC TTCACGGCAT CCGGGAGCTG 
AGTCCGCCTC CTATCAATGC GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dgkd cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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