ptch1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ptch1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptch1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa48750 XM_031890957.1
Latest version!
Xenopus tropicalis patched 1 (ptch1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48751 XM_031890958.1
Latest version!
Xenopus tropicalis patched 1 (ptch1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48752 XM_031890959.1
Latest version!
Xenopus tropicalis patched 1 (ptch1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa03925 XM_002934973.4
Latest version!
Xenopus tropicalis patched 1 (ptch1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa48750
    Clone ID Related Accession (Same CDS sequence) XM_031890957.1
    Accession Version XM_031890957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein patched homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGCCTATGC CGCGGAATTG GGTGGCAGTG GGGAGCCAGC TGCCCAGCCG 
    AGGGTAGTCA GGAGGAGAGG AAGGAGCAGG GGGGAGCGCA GAATAGTAGC GCCGCCTGAC
    CAGGACTATC TGCAGCGGCC GAGCTACTGC GATGCGAATT TCGCCCTTCA GCAGATATCA
    GAGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCGTT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACGGTCCACT
    GCTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746817.1
    CDS1200..5510
    Translation

    Target ORF information:

    RefSeq Version XM_031890957.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis patched 1 (ptch1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGCCTATGC CGCGGAATTG GGTGGCAGTG GGGAGCCAGC TGCCCAGCCG 
    AGGGTAGTCA GGAGGAGAGG AAGGAGCAGG GGGGAGCGCA GAATAGTAGC GCCGCCTGAC
    CAGGACTATC TGCAGCGGCC GAGCTACTGC GATGCGAATT TCGCCCTTCA GCAGATATCA
    GAGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCGTT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACGGTCCACT
    GCTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48751
    Clone ID Related Accession (Same CDS sequence) XM_031890958.1
    Accession Version XM_031890958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein patched homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA 
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCGTT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACGGTCCACT
    GCTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746818.1
    CDS177..4307
    Translation

    Target ORF information:

    RefSeq Version XM_031890958.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis patched 1 (ptch1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA 
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCGTT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACGGTCCACT
    GCTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48752
    Clone ID Related Accession (Same CDS sequence) XM_031890959.1
    Accession Version XM_031890959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein patched homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCATACA TGCCAGGTAA AGGCTTTTTG CAGTGGACAA ACTTTGACCC TTTGGGGTTG 
    CTGGAAGAGT TAAAGAAAGG AAAACTTCAT ATAGACACCT GGGAGGAAAT GATAAACAAG
    GCAGAAGTTG GCCACGGTTA TATGGACAGA CCTTGTTTGA ACCCATCAGA TGATAAGTGT
    CCAAACACTG CACCCAACAA AAATTCAACC AAACCTGTAG ATGTAGCCCT TATTCTAAAT
    GGTGGATGCT ATGGACTCTC AAGAAAATAT ATGCACTGGC AAGAGGAGTT GATCATTGGT
    GGCACAGTGA AGAATGCTAG TGGACAAAGT GTCAGCGCCC TGGCATTGCA GACTATGTTT
    CAACTAATGA CTCCCAAACA AATGTATGAG CACTTCAAGG GTTATGAAGA GGTTTCCCAC
    ATAAATTGGA ATGAAGACAA AGCGGCTGCA ATCTTGGAGG CCTGGCAAAG GACCTATGTT
    CAGGTTGTAC ATCAAAGTGT TCCACAGAAC TCCTCACAGA AAGTTCTCCC TTTTACTACA
    ACTACACTGG ATGATATTCT CAAGTCATTC TCCGATGTGA GCGTTATCAG AGTTGCAAGT
    GGCTATCTGC TGATGCTTGC CTATGCCTGT TTAACCATGC TGAGGTGGGA CTGTGCCAAG
    TCTCAGGGTG CCGTAGGGCT AGCAGGAGTC TTGCTGGTTG CGCTTTCAGT GGCTGCTGGA
    TTGGGTCTGT GTTCATTGAT TGGAATTTCC TTTAATGCAG CAACAACTCA GGTTTTGCCT
    TTTCTCGCTC TTGGGGTTGG TGTAGATGAT GTCTTCCTTT TGGCACATGC TTTTAGTGAA
    ACTGGGCAGA ATAAAAGGAT TCCATTTGAG GACAGGACTG GTGAGTGTCT AAAGCGGACA
    GGAGCAAGTG TAGCCCTTAC GTCTATTAGC AATGTGACTG CATTCTTCAT GGCTGCCTTG
    ATACCTATTC CTGCACTGCG GGCATTTTCC CTCCAAGCTG CTGTTGTTGT GGTGTTTAAT
    TTTGCCATGG TCTTGCTGAT ATTTCCCGCT ATTCTAAGCA TGGACCTCTA TCGCAGGGAA
    GTCCGCAGGC TTGATATCTT CTGTTGCTTC AGCAGTCCCT GCGTGAGCAG AGTGATTCAG
    ATTGAACCCC AGGCCTATAC GGACAACAAT GACAACACAC GTTACAGCCC ACCTCCACCC
    TACAGCAGTC ACAGCTTTGC TCATGAAACA CAAATCACAA TGCAGTCAAC AGTCCAGCTA
    CGGACCGAAT ATGATCCACG AACGCAGTTG TATTACACCA CAGCAGAGCC TCGATCTGAA
    ATATCAGTGC AGCCAGCCGC ACCATCACCT CAGGATGTAA GCGGCCAGAC TCCAGAGAGC
    ACCAGTTCCA CTCGAGACCT GCTTTCTCCA TTCTCTGAAT CCGGAGTGCA CTGCATAGAG
    CCCCCATGCA CAAAGTGGAC ATTGTCTTCC TTTGCAGAAA AGCACTACGC TCCATTCCTG
    TTAAAGCCAA ACACTAAAGT TGCGGTCATA TTGGTTTTCT TAGCCCTTCT TAGTGTCAGC
    CTTTATGGAA CTACTCGAGT GCGAGATGGA CTAGATTTGA CCGACATTGT ACCGAGAGAA
    ACCAGGGAAT ATGACTTTAT TGCCACGCAG TTCAAGTACT TTTCGTTTTA CCACATGTAT
    GTAGTAACAC AGAAAGCAGA CTATCCTCGT GCTCAGCGCT TGCTTTATGA ACTTCACAAG
    AGCTTTGCCG GGGTGAAATA TGTGTTGCTG GAGGGTAACA AACAGCTTCC TAGGATGTGG
    CTGCATTACT TTCGAGATTG GCTGCAAGGA TTGCAAGATA CGTTTGATCG TGAATGGGAG
    GCTGGGAAAA TTACACACAA TGACTACAGA AATGCTTCTG ATGATGCTGT GCTTGCTTAC
    AAGCTCCTAA TACAGACAGG CAATTCTGAT AAACCTATTA ACCTCAACCA GCTGACTAAA
    CAGCGGTTGG TGGATACAGA TGGGATTATT CAGCCAAATG CTTTCTACAT CTACTTAACA
    GCCTGGGTGA GTAATGACCC TGTAGCCTAT GCTGCTTCCC AGGCCAACAT CCGCCCACAC
    CCTCCTGAGT GGCTCCACGA CAAAGCAGAC GATAGGCCTG AGACCAGGAC AATTCGTGCA
    GCTGAACCTA TAGAATATGT CCAGTTTCCT TTCTACCTCA ATGGTCTACG GGAAACATCC
    GACTTTGTGG AGGCTATAGA AAAAGTGAGG GCAATCTGTA ACAACTATAC AAGCCTTGGG
    GTGTCGAGCT ACCCAAATGG TTACCCTTTC CTGTTCTGGG AGCAATACAT TGGTCTCCGT
    CACTGGCTGC TGTTGTCCAT CAGTGTGGTT CTGGCCTGTA CGTTTCTGGT GTGTGCTGTC
    TTTCTGCTGA ACCCTTGGAC TGCTGGAATC ATTGTCATGG TGTTGGCCCT TATGACAGTG
    GAACTGTTTG GCATGATGGG ACTCATAGGG ATTAAACTGA GTGCAGTGCC TGTAGTTATC
    TTGATAGCTT CAGTTGGAAT TGGAGTGGAA TTCACTGTTC ATGTTGCTTT GGCATTCCTC
    ACTGCTATTG GGGACAAGAA TCGCAGAGCT GTTCTTGCTT TGGAGCACAT GTTTGCACCT
    GTTCTGGATG GGGCTGTATC TACTCTATTG GGAGTTCTAA TGCTTGCTGG ATCAGAGTTC
    GACTTTATTG TCAGGTACTT TTTTGCTGTC CTGGCAATTT TAACTCTTTT GGGTGTATTA
    AACGGCTTGG TGCTACTTCC TGTACTTCTG TCTTATTTTG GGCCTTATCC TGAGGTCTCT
    CCAACTAATG GCAGAGATAG ACTTGAGTTT CCTCAAGAGC CACCACCAAA TATTGTAAGA
    TTCCCTGTGC ATCCTAGACC GACGCAGAAT GGGTCTGATT CCTCAGATTC AGAATATAGT
    TCTCAGACAA CTGTATCAGG AATCAGTGAG GAGCTTCATC AGTATGATTC CCAGCAGTGT
    TCCAGAGCAT CAACAAATCA AGTCTTCCTG GAAGCCAGAA GAAATCCAGC CTTTCCACGG
    TCCACTGCTG TACAACAGAA CAATAGGCAT AATCCGCCAA ATCAAAACAC ACCACCCAAC
    CAAGAACCCA GAACTCAACA ACAATGGCGA TCCAATAGGA ACCAGAGATG GGATTCAACA
    GAAGGATCAA GGCCACCTCC ATACAGACCT CGCAGAGATG CCTTTGCATC TTCTACTGAA
    GAGCATGCTG GTCCTAGTAC CAGCAGGGAC AGATCAGGGC ACAGATCCCA CTCCCATAAC
    ATGCGAAAAA CTGCCTTTGC TCCAGGAGGT GCTTCAGTGC CTGCATATGG TCAACCTATT
    ACTACTGTGA CTGCCTCTGC TTCAGTTACT GTTGCTGTGC ACCCTTCTGT GTGTAGCCAC
    AGCTCCCATG GTAGCTACCC CTTTTATGGT GGTTACCACG AAAATGACCA TGGACATTTT
    GAGGACCCTC ATGTGCCTTT TAATGTACGC TATGAAAGAA GGGACTCTAA AGTAGAAGTA
    ATAGAGCTGC AAGATGTTGA ATGCGAAGAA AGGACACAGG GAAATGCCTC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746819.1
    CDS242..3838
    Translation

    Target ORF information:

    RefSeq Version XM_031890959.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis patched 1 (ptch1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCATACA TGCCAGGTAA AGGCTTTTTG CAGTGGACAA ACTTTGACCC TTTGGGGTTG 
    CTGGAAGAGT TAAAGAAAGG AAAACTTCAT ATAGACACCT GGGAGGAAAT GATAAACAAG
    GCAGAAGTTG GCCACGGTTA TATGGACAGA CCTTGTTTGA ACCCATCAGA TGATAAGTGT
    CCAAACACTG CACCCAACAA AAATTCAACC AAACCTGTAG ATGTAGCCCT TATTCTAAAT
    GGTGGATGCT ATGGACTCTC AAGAAAATAT ATGCACTGGC AAGAGGAGTT GATCATTGGT
    GGCACAGTGA AGAATGCTAG TGGACAAAGT GTCAGCGCCC TGGCATTGCA GACTATGTTT
    CAACTAATGA CTCCCAAACA AATGTATGAG CACTTCAAGG GTTATGAAGA GGTTTCCCAC
    ATAAATTGGA ATGAAGACAA AGCGGCTGCA ATCTTGGAGG CCTGGCAAAG GACCTATGTT
    CAGGTTGTAC ATCAAAGTGT TCCACAGAAC TCCTCACAGA AAGTTCTCCC TTTTACTACA
    ACTACACTGG ATGATATTCT CAAGTCATTC TCCGATGTGA GCGTTATCAG AGTTGCAAGT
    GGCTATCTGC TGATGCTTGC CTATGCCTGT TTAACCATGC TGAGGTGGGA CTGTGCCAAG
    TCTCAGGGTG CCGTAGGGCT AGCAGGAGTC TTGCTGGTTG CGCTTTCAGT GGCTGCTGGA
    TTGGGTCTGT GTTCATTGAT TGGAATTTCC TTTAATGCAG CAACAACTCA GGTTTTGCCT
    TTTCTCGCTC TTGGGGTTGG TGTAGATGAT GTCTTCCTTT TGGCACATGC TTTTAGTGAA
    ACTGGGCAGA ATAAAAGGAT TCCATTTGAG GACAGGACTG GTGAGTGTCT AAAGCGGACA
    GGAGCAAGTG TAGCCCTTAC GTCTATTAGC AATGTGACTG CATTCTTCAT GGCTGCCTTG
    ATACCTATTC CTGCACTGCG GGCATTTTCC CTCCAAGCTG CTGTTGTTGT GGTGTTTAAT
    TTTGCCATGG TCTTGCTGAT ATTTCCCGCT ATTCTAAGCA TGGACCTCTA TCGCAGGGAA
    GTCCGCAGGC TTGATATCTT CTGTTGCTTC AGCAGTCCCT GCGTGAGCAG AGTGATTCAG
    ATTGAACCCC AGGCCTATAC GGACAACAAT GACAACACAC GTTACAGCCC ACCTCCACCC
    TACAGCAGTC ACAGCTTTGC TCATGAAACA CAAATCACAA TGCAGTCAAC AGTCCAGCTA
    CGGACCGAAT ATGATCCACG AACGCAGTTG TATTACACCA CAGCAGAGCC TCGATCTGAA
    ATATCAGTGC AGCCAGCCGC ACCATCACCT CAGGATGTAA GCGGCCAGAC TCCAGAGAGC
    ACCAGTTCCA CTCGAGACCT GCTTTCTCCA TTCTCTGAAT CCGGAGTGCA CTGCATAGAG
    CCCCCATGCA CAAAGTGGAC ATTGTCTTCC TTTGCAGAAA AGCACTACGC TCCATTCCTG
    TTAAAGCCAA ACACTAAAGT TGCGGTCATA TTGGTTTTCT TAGCCCTTCT TAGTGTCAGC
    CTTTATGGAA CTACTCGAGT GCGAGATGGA CTAGATTTGA CCGACATTGT ACCGAGAGAA
    ACCAGGGAAT ATGACTTTAT TGCCACGCAG TTCAAGTACT TTTCGTTTTA CCACATGTAT
    GTAGTAACAC AGAAAGCAGA CTATCCTCGT GCTCAGCGCT TGCTTTATGA ACTTCACAAG
    AGCTTTGCCG GGGTGAAATA TGTGTTGCTG GAGGGTAACA AACAGCTTCC TAGGATGTGG
    CTGCATTACT TTCGAGATTG GCTGCAAGGA TTGCAAGATA CGTTTGATCG TGAATGGGAG
    GCTGGGAAAA TTACACACAA TGACTACAGA AATGCTTCTG ATGATGCTGT GCTTGCTTAC
    AAGCTCCTAA TACAGACAGG CAATTCTGAT AAACCTATTA ACCTCAACCA GCTGACTAAA
    CAGCGGTTGG TGGATACAGA TGGGATTATT CAGCCAAATG CTTTCTACAT CTACTTAACA
    GCCTGGGTGA GTAATGACCC TGTAGCCTAT GCTGCTTCCC AGGCCAACAT CCGCCCACAC
    CCTCCTGAGT GGCTCCACGA CAAAGCAGAC GATAGGCCTG AGACCAGGAC AATTCGTGCA
    GCTGAACCTA TAGAATATGT CCAGTTTCCT TTCTACCTCA ATGGTCTACG GGAAACATCC
    GACTTTGTGG AGGCTATAGA AAAAGTGAGG GCAATCTGTA ACAACTATAC AAGCCTTGGG
    GTGTCGAGCT ACCCAAATGG TTACCCTTTC CTGTTCTGGG AGCAATACAT TGGTCTCCGT
    CACTGGCTGC TGTTGTCCAT CAGTGTGGTT CTGGCCTGTA CGTTTCTGGT GTGTGCTGTC
    TTTCTGCTGA ACCCTTGGAC TGCTGGAATC ATTGTCATGG TGTTGGCCCT TATGACAGTG
    GAACTGTTTG GCATGATGGG ACTCATAGGG ATTAAACTGA GTGCAGTGCC TGTAGTTATC
    TTGATAGCTT CAGTTGGAAT TGGAGTGGAA TTCACTGTTC ATGTTGCTTT GGCATTCCTC
    ACTGCTATTG GGGACAAGAA TCGCAGAGCT GTTCTTGCTT TGGAGCACAT GTTTGCACCT
    GTTCTGGATG GGGCTGTATC TACTCTATTG GGAGTTCTAA TGCTTGCTGG ATCAGAGTTC
    GACTTTATTG TCAGGTACTT TTTTGCTGTC CTGGCAATTT TAACTCTTTT GGGTGTATTA
    AACGGCTTGG TGCTACTTCC TGTACTTCTG TCTTATTTTG GGCCTTATCC TGAGGTCTCT
    CCAACTAATG GCAGAGATAG ACTTGAGTTT CCTCAAGAGC CACCACCAAA TATTGTAAGA
    TTCCCTGTGC ATCCTAGACC GACGCAGAAT GGGTCTGATT CCTCAGATTC AGAATATAGT
    TCTCAGACAA CTGTATCAGG AATCAGTGAG GAGCTTCATC AGTATGATTC CCAGCAGTGT
    TCCAGAGCAT CAACAAATCA AGTCTTCCTG GAAGCCAGAA GAAATCCAGC CTTTCCACGG
    TCCACTGCTG TACAACAGAA CAATAGGCAT AATCCGCCAA ATCAAAACAC ACCACCCAAC
    CAAGAACCCA GAACTCAACA ACAATGGCGA TCCAATAGGA ACCAGAGATG GGATTCAACA
    GAAGGATCAA GGCCACCTCC ATACAGACCT CGCAGAGATG CCTTTGCATC TTCTACTGAA
    GAGCATGCTG GTCCTAGTAC CAGCAGGGAC AGATCAGGGC ACAGATCCCA CTCCCATAAC
    ATGCGAAAAA CTGCCTTTGC TCCAGGAGGT GCTTCAGTGC CTGCATATGG TCAACCTATT
    ACTACTGTGA CTGCCTCTGC TTCAGTTACT GTTGCTGTGC ACCCTTCTGT GTGTAGCCAC
    AGCTCCCATG GTAGCTACCC CTTTTATGGT GGTTACCACG AAAATGACCA TGGACATTTT
    GAGGACCCTC ATGTGCCTTT TAATGTACGC TATGAAAGAA GGGACTCTAA AGTAGAAGTA
    ATAGAGCTGC AAGATGTTGA ATGCGAAGAA AGGACACAGG GAAATGCCTC AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03925
    Clone ID Related Accession (Same CDS sequence) XM_002934973.4
    Accession Version XM_002934973.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein patched homolog 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934973.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGCCTATGC CGCGGAATTG GGTGGCAGTG GGGAGCCAGC TGCCCAGCCG 
    AGGGTAGTCA GGAGGAGAGG AAGGAGCAGG GGGGAGCGCA GAATAGTAGC GCCGCCTGAC
    CAGGACTATC TGCAGCGGCC GAGCTACTGC GATGCGAATT TCGCCCTTCA GCAGATTTCA
    GAGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCATT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACATATATAT
    ATTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935019.2
    CDS1207..5517
    Translation

    Target ORF information:

    RefSeq Version XM_002934973.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis patched 1 (ptch1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934973.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTCGG CTGCCTATGC CGCGGAATTG GGTGGCAGTG GGGAGCCAGC TGCCCAGCCG 
    AGGGTAGTCA GGAGGAGAGG AAGGAGCAGG GGGGAGCGCA GAATAGTAGC GCCGCCTGAC
    CAGGACTATC TGCAGCGGCC GAGCTACTGC GATGCGAATT TCGCCCTTCA GCAGATTTCA
    GAGGGGAAGG CTACAGGAAG AAAAGCTCCA TTGTGGCTAA GAGCATTCTT TCAAAGACAA
    TTGTTTAAAC TTGGTTGTTA CATACAAAAA AACTGCGGAA AATTTCTGGT TGTGGGCCTT
    TTGATATTTG GTGCTTTCGC TGTTGGATTA AGAGCAGCTA ATTTAGAGAC CAATGTGGAA
    GAACTGTGGG TTGAAGTTGG TGGAAGAGTA AGCCGCGAAT TAAATTACAC TCGCCAGAAG
    ATAGGAGAGG AGGCTATGTT TAATCCACAG CTTATGATCC AGACTCCTCT TGAAGATGGT
    GCTAATGTCC TGACAACAGA AGCTCTCCTG CAACATCTGC ACTCTGCACT TGAAGCCACC
    AAGGTGCAAG TCTACATGTA CAACAAACCT TGGAAATTGG AAAAATTATG CTTTAAATCA
    GGTGAACTTA TTACAGAAGC AGGATATGTA AGCCAGATTA TAGAATCATT GTATCCATGT
    TTAATCATCA CACCACTGGA TTGCTTTTGG GAAGGCGCAA AGCTTCACTC TGGAATGGCA
    TACATGCCAG GTAAAGGCTT TTTGCAGTGG ACAAACTTTG ACCCTTTGGG GTTGCTGGAA
    GAGTTAAAGA AAGGAAAACT TCATATAGAC ACCTGGGAGG AAATGATAAA CAAGGCAGAA
    GTTGGCCACG GTTATATGGA CAGACCTTGT TTGAACCCAT CAGATGATAA GTGTCCAAAC
    ACTGCACCCA ACAAAAATTC AACCAAACCT GTAGATGTAG CCCTTATTCT AAATGGTGGA
    TGCTATGGAC TCTCAAGAAA ATATATGCAC TGGCAAGAGG AGTTGATCAT TGGTGGCACA
    GTGAAGAATG CTAGTGGACA AAGTGTCAGC GCCCTGGCAT TGCAGACTAT GTTTCAACTA
    ATGACTCCCA AACAAATGTA TGAGCACTTC AAGGGTTATG AAGAGGTTTC CCACATAAAT
    TGGAATGAAG ACAAAGCGGC TGCAATCTTG GAGGCCTGGC AAAGGACCTA TGTTCAGGTT
    GTACATCAAA GTGTTCCACA GAACTCCTCA CAGAAAGTTC TCCCTTTTAC TACAACTACA
    CTGGATGATA TTCTCAAGTC ATTCTCCGAT GTGAGCGTTA TCAGAGTTGC AAGTGGCTAT
    CTGCTGATGC TTGCCTATGC CTGTTTAACC ATGCTGAGGT GGGACTGTGC CAAGTCTCAG
    GGTGCCGTAG GGCTAGCAGG AGTCTTGCTG GTTGCGCTTT CAGTGGCTGC TGGATTGGGT
    CTGTGTTCAT TGATTGGAAT TTCCTTTAAT GCAGCAACAA CTCAGGTTTT GCCTTTTCTC
    GCTCTTGGGG TTGGTGTAGA TGATGTCTTC CTTTTGGCAC ATGCTTTTAG TGAAACTGGG
    CAGAATAAAA GGATTCCATT TGAGGACAGG ACTGGTGAGT GTCTAAAGCG GACAGGAGCA
    AGTGTAGCCC TTACGTCTAT TAGCAATGTG ACTGCATTCT TCATGGCTGC CTTGATACCT
    ATTCCTGCAC TGCGGGCATT TTCCCTCCAA GCTGCTGTTG TTGTGGTGTT TAATTTTGCC
    ATGGTCTTGC TGATATTTCC CGCTATTCTA AGCATGGACC TCTATCGCAG GGAAGTCCGC
    AGGCTTGATA TCTTCTGTTG CTTCAGCAGT CCCTGCGTGA GCAGAGTGAT TCAGATTGAA
    CCCCAGGCCT ATACGGACAA CAATGACAAC ACACGTTACA GCCCACCTCC ACCCTACAGC
    AGTCACAGCT TTGCTCATGA AACACAAATC ACAATGCAGT CAACAGTCCA GCTACGGACC
    GAATATGATC CACGAACGCA GTTGTATTAC ACCACAGCAG AGCCTCGATC TGAAATATCA
    GTGCAGCCAG CCGCACCATC ACCTCAGGAT GTAAGCGGCC AGACTCCAGA GAGCACCAGT
    TCCACTCGAG ACCTGCTTTC TCCATTCTCT GAATCCGGAG TGCACTGCAT AGAGCCCCCA
    TGCACAAAGT GGACATTGTC TTCCTTTGCA GAAAAGCACT ACGCTCCATT CCTGTTAAAG
    CCAAACACTA AAGTTGCGGT CATATTGGTT TTCTTAGCCC TTCTTAGTGT CAGCCTTTAT
    GGAACTACTC GAGTGCGAGA TGGACTAGAT TTGACCGACA TTGTACCGAG AGAAACCAGG
    GAATATGACT TTATTGCCAC GCAGTTCAAG TACTTTTCGT TTTACCACAT GTATGTAGTA
    ACACAGAAAG CAGACTATCC TCGTGCTCAG CGCTTGCTTT ATGAACTTCA CAAGAGCTTT
    GCCGGGGTGA AATATGTGTT GCTGGAGGGT AACAAACAGC TTCCTAGGAT GTGGCTGCAT
    TACTTTCGAG ATTGGCTGCA AGGATTGCAA GATACGTTTG ATCGTGAATG GGAGGCTGGG
    AAAATTACAC ACAATGACTA CAGAAATGCT TCTGATGATG CTGTGCTTGC TTACAAGCTC
    CTAATACAGA CAGGCAATTC TGATAAACCT ATTAACCTCA ACCAGCTGAC TAAACAGCGG
    TTGGTGGATA CAGATGGGAT TATTCAGCCA AATGCTTTCT ACATCTACTT AACAGCCTGG
    GTGAGTAATG ACCCTGTAGC CTATGCTGCT TCCCAGGCCA ACATCCGCCC ACACCCTCCT
    GAGTGGCTCC ACGACAAAGC AGACGATAGG CCTGAGACCA GGACAATTCG TGCAGCTGAA
    CCTATAGAAT ATGTCCAGTT TCCTTTCTAC CTCAATGGTC TACGGGAAAC ATCCGACTTT
    GTGGAGGCTA TAGAAAAAGT GAGGGCAATC TGTAACAACT ATACAAGCCT TGGGGTGTCG
    AGCTACCCAA ATGGTTACCC TTTCCTGTTC TGGGAGCAAT ACATTGGTCT CCGTCACTGG
    CTGCTGTTGT CCATCAGTGT GGTTCTGGCC TGTACGTTTC TGGTGTGTGC TGTCTTTCTG
    CTGAACCCTT GGACTGCTGG AATCATTGTC ATGGTGTTGG CCCTTATGAC AGTGGAACTG
    TTTGGCATGA TGGGACTCAT AGGGATTAAA CTGAGTGCAG TGCCTGTAGT TATCTTGATA
    GCTTCAGTTG GAATTGGAGT GGAATTCACT GTTCATGTTG CTTTGGCATT CCTCACTGCT
    ATTGGGGACA AGAATCGCAG AGCTGTTCTT GCTTTGGAGC ACATGTTTGC ACCTGTTCTG
    GATGGGGCTG TATCTACTCT ATTGGGAGTT CTAATGCTTG CTGGATCAGA GTTCGACTTT
    ATTGTCAGGT ACTTTTTTGC TGTCCTGGCA ATTTTAACTC TTTTGGGTGT ATTAAACGGC
    TTGGTGCTAC TTCCTGTACT TCTGTCTTAT TTTGGGCCTT ATCCTGAGGT CTCTCCAACT
    AATGGCAGAG ATAGACTTGA GTTTCCTCAA GAGCCACCAC CAAATATTGT AAGATTCCCT
    GTGCATCCTA GACCGACGCA GAATGGGTCT GATTCCTCAG ATTCAGAATA TAGTTCTCAG
    ACAACTGTAT CAGGAATCAG TGAGGAGCTT CATCAGTATG ATTCCCAGCA GTGTTCCAGA
    GCATCAACAA ATCAAGTCTT CCTGGAAGCC AGAAGAAATC CAGCCTTTCC ACATATATAT
    ATTGTACAAC AGAACAATAG GCATAATCCG CCAAATCAAA ACACACCACC CAACCAAGAA
    CCCAGAACTC AACAACAATG GCGATCCAAT AGGAACCAGA GATGGGATTC AACAGAAGGA
    TCAAGGCCAC CTCCATACAG ACCTCGCAGA GATGCCTTTG CATCTTCTAC TGAAGAGCAT
    GCTGGTCCTA GTACCAGCAG GGACAGATCA GGGCACAGAT CCCACTCCCA TAACATGCGA
    AAAACTGCCT TTGCTCCAGG AGGTGCTTCA GTGCCTGCAT ATGGTCAACC TATTACTACT
    GTGACTGCCT CTGCTTCAGT TACTGTTGCT GTGCACCCTT CTGTGTGTAG CCACAGCTCC
    CATGGTAGCT ACCCCTTTTA TGGTGGTTAC CACGAAAATG ACCATGGACA TTTTGAGGAC
    CCTCATGTGC CTTTTAATGT ACGCTATGAA AGAAGGGACT CTAAAGTAGA AGTAATAGAG
    CTGCAAGATG TTGAATGCGA AGAAAGGACA CAGGGAAATG CCTCAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.