spef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03384 XM_004910428.2
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35202 XM_012967670.2
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35203 XM_012967689.2
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35204 XM_012967692.2
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa42251 XM_018090829.1
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49220 XM_031894698.1
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49221 XM_031894702.1
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa49222 XM_031894707.1
Latest version!
Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03384
    Clone ID Related Accession (Same CDS sequence) XM_004910428.2
    Accession Version XM_004910428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910485.1
    CDS134..5392
    Translation

    Target ORF information:

    RefSeq Version XM_004910428.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35202
    Clone ID Related Accession (Same CDS sequence) XM_012967670.2
    Accession Version XM_012967670.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823124.1
    CDS124..5382
    Translation

    Target ORF information:

    RefSeq Version XM_012967670.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967670.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35203
    Clone ID Related Accession (Same CDS sequence) XM_012967689.2
    Accession Version XM_012967689.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT
    GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA
    TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA
    AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG
    AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT
    AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA
    AAGGCTCTCA CTGGCTCACA CACTAATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT
    GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT
    GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA
    ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG
    AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC
    CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT
    GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC
    GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA
    GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT
    TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA
    TCTCCAAGAG AATCTCGAAA ACAGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA
    GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA
    AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA
    CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA
    ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC
    AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT
    ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT
    CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA
    GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC
    AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT
    CACACCGGGA TACTTATTAA CCACTTTTTC TCTCTGATGC AGGCGGAAGT TGATCGCTTC
    CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT
    GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG
    GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA
    CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA
    GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT
    GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG
    GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT
    GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG
    AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823143.1
    CDS124..5379
    Translation

    Target ORF information:

    RefSeq Version XM_012967689.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967689.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT
    GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA
    TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA
    AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG
    AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT
    AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA
    AAGGCTCTCA CTGGCTCACA CACTAATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT
    GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT
    GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA
    ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG
    AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC
    CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT
    GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC
    GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA
    GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT
    TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA
    TCTCCAAGAG AATCTCGAAA ACAGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA
    GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA
    AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA
    CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA
    ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC
    AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT
    ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT
    CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA
    GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC
    AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT
    CACACCGGGA TACTTATTAA CCACTTTTTC TCTCTGATGC AGGCGGAAGT TGATCGCTTC
    CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT
    GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG
    GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA
    CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA
    GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT
    GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG
    GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT
    GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG
    AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35204
    Clone ID Related Accession (Same CDS sequence) XM_012967692.2
    Accession Version XM_012967692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823146.1
    CDS124..5379
    Translation

    Target ORF information:

    RefSeq Version XM_012967692.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012967692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42251
    Clone ID Related Accession (Same CDS sequence) XM_018090829.1
    Accession Version XM_018090829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCAGAAC AGCACGGAGT GGCTACCCGG CTTCTGTATC AGTTGTTCAT AGCATTGCAG 
    AAGAAAAAGA AGGCAGGATT GACAGGAGTA GCCATGGAGA CATTGAGACC AGCTGCTCCA
    GCAAAGCTTC AGAGCATTGG AACTGAGATG TACAGAGAGC GTTTGAAAAC CATAATCCCC
    CGCCAAAGTG AAATCAGCCT GCAGAGGGTG TCAGAACAAT TTGACCTGAA AGCAAAAGAA
    ATCGAAGACA AAGTTGCACA GATTCAAAAT GATGAACTTC GGAAAATACA GAAAATACAA
    GAAGAGCTAA GAATTCAGGA TATTGAAAAG CTGCGACGAG CTAGAAGGAG ACAAACCGAA
    ATAATGGCAC GAATTCAAGC AGCAATTGTT CAGATTCCAA AACCACCTCA AAACCGCACA
    TTGAAGGCCA TCGAAGCACA AAAGATGCTG AAAAAGAAGA AAGAAGCTGA GGAAGTTTAT
    ACAGAAATCA CAAAGTTTGA AAAAGGAGTG AAAGATGGAT CTGTTGGAAG CTTGTCTGCT
    AATACCAGGG GCCCAACACT CCAGAGATTA AGGTCATCAG TTAACATTGG CACTGCTTTG
    CTAAAACTGG ATACTAATGA TGATTACGTA AGGAAAATTC AGAAGCGATT AGAGGAGGAT
    TCTGTAGCAC GTGAGCAGCG CGAAAAGAGA CGTCGGCGGA TTCTTATGGA ACAGCTTGTG
    GGTCATGGGG CACAAGAGGA AGCTTTCCGG GAGGAACAAC TGATAAACCG ATTAATGAGA
    CAGTCCCAGC AAGAGCGGAG AATTGCAGTT CAGCTTATGC ACGCGCGCCA TGAAAAGGAA
    GTTATGCAAC AGAACCGAAT GTTCCGGGAG AAGCAATATC AGGAGAGGCG CGAGAGGGAG
    TTCCAAGAAG CTCTTGACAG AGAAGCGGCG TTGCAGAAAC AAGCGATGCT CGACCACAAA
    GAAGAAATAC GCAAAGTGCA GGAGCTCCAT GACCAGATAG CAGCGGAGCG TGCAGAGGCT
    CGCTATAAAA AGCACTATAA CCTGTGCCAG GAAATCATGA ATCAGATTTT AGACTTTGTT
    ATTAAAACAG GGGAATACAG AGAGCTAACA AACAGACTTA TACCTGTTAA ATTGATGCGG
    GAGTGGAAAG AACTATTCTT CGCTGGTTAC CCGCTTTACG ACAAAGCACC TATAGATGCC
    CTCCCTTCCG ACCCAACTGC AGAGCAGCTG CAAGAAACAG AAAAGCAGAG TCTGTTAGAT
    GAGAAGGATT ATGAAGAATA CAAGACCATG ACAGGGGAAT GGGGCCCACC CGAAGCCAGC
    AGTGTTAAAG CACCTGCACC GAACAACAAC ATATTGGGCT ATGTGGTTCA TAGACTGTTG
    GAAATTGTCA GCCCCCCATT GGCACCCACA CCGCCACCTG TGTTTCCCCC CTTCCCCATC
    AAGGGTTGTG TTCTTGGGAA GATTTACTCT GGGAAGAGCA CGTGCCTGAA ACATCTGGCA
    CAGGTGTATA GCATCCAAGT TCTCTCTGTG GACACCCTTC TGGAGGAAGC TATTCAAGCA
    TTTAATGACG GGGAACTGGA AAAGACCGAG GATGTTCTGA GGCCCATACA AGAAGGAGCA
    GATAAAGAGA ACAAGGTACC CATAGACAGT CCTGCTCCTG TTACTAATGG CGAAGAGATA
    CCTGTTGTGG GAAGTGGCAG CAAGCCTGCA TCTGCAGAAG CCCGTGAATC AGATAAAGAG
    TTATCTCTAC ATCAAATATT AAAGCCTGCA ATAGAAGATA CTGTGCCAAA GCTCTCTGCA
    CGGGCACAGC TTGGTGGACT GGCAGAGACA CATTTAAAGA GTGGGAAGAG TGTCCCAGAT
    GAGCTGCTTA TCAGTATACT TGTTGAAGCC ATCAATCGCA TCCCAGCTAA CACAGGGTGG
    ATCATGGAGG GATTCCCTAT GACGGAAACT CAGGCCACAT TATTTGAAAA GGCTCTCACT
    GGCTCACACA CTAATTTGAA TGGAATAAAG GGGAATAATA ACAAGTTTGC CTCTTTAGTA
    ACAGATCCAG CAGCACCAAA GGATCTCCCT GTGGCTCCAT CTGCTCTTGA CTTTGCTGTG
    TTAATAGAAA TATCAGACAA TGAAGTGTTA CAGCGCATGT CTACAATAAT CAGTGAAATA
    GATAAATCTG GAGAGAAAAT TGATAGTCCT CCCTGTATTC AAACTACGAG AATGGAAGCA
    GATCAAACAA TGGACCAGAT ACAGCACAGA ATTACAGGAT TTCTTGACCA CTGGAAGAAT
    TTAGAAATGT GGTTTTCAGA GCAGCAGAAT ATTCTAATCA AAGTTAATGG GGAAGTGGAA
    GTTGGCGTTC TGTGCAAGAG AATAGAAGAA GTATACTTGA CAGCTCTCGT CAATAAGCAA
    AACCGAGGCA AAAAAAACGA AAAGAAAGAA GATCTCCCAC CACCAGAAGT AGCCGCACCA
    ACACCGCCAC CGCCATCTCC TCCTCCAGCT CCAGAAGCCA CTAACAATTT ACGACCTCCA
    TCAGCTTCAA GCAAGGAATC AGCAAAAAAA AGCAGGAAAG GCTCCAAATC TCCAAGAGAA
    TCTCGAAAAC AGTCTGACAC CTCGAAGGAC AAGAAGGACA AGAAACCAGA GACACCTAAA
    GGAAAAGACT CTCGAAAATC TGGTTCACCA CGTGGAAGGT CTCCTGGAAA GAAGGCTAAG
    TCTGTACCTG CCAGCCCTGA ACCAGTCGCT GTCACTCCTG TTGGACCACC ACCCATCCAG
    CCTGGATCCC CTGAATGGGT GTACGTAAAT GAGCCACTTC CGAAAGAAAT CCCAGCATTC
    CTTGTACCAT ATTGGAAGAC TATAGAAAAT GTCTATGGAA TGACCATCAA AACTGTGCTG
    AGGAATCTGA GGCAGGAACG ACTAACTGTG ATTCACTATT TATATGATAT CAGGGTTCAT
    TTGAAGGATT ACTTGAAGCG GCCGGATCAC AAGCAGGAAT TCGTGTCTCA GTGGCAGTCG
    GACTTTAATT CTACCGCAGA CGACTTGTGG GAGGACGAGG AAACAAAAGC AGAGCTTCAT
    CAAAGAGCTG ATGATCTCCG GGACCGTCTG TGGGACATAT GTGACAACAG GAAGGAGGAG
    GCAGAACAAG AGCGCACAGA CATTATGAAT GATGGGTGGC TACAGGATCA CACCGGGATA
    CTTATTAACC ACTTTTTCTC TCTGATGCAG GCGGAAGTTG ATCGCTTCCA AGACACCACA
    AGACTTCTCA GTGATTACTA CCAAGGAATG GAGGGACAAG CCCCATCTGA GGCCAGCCAT
    ACCTTTTCCC GTATCCCACT GCTGGACATT GCTAACTCAA GCACGCAGGG GGATTCTGAG
    AACCCTAAAA GAATTCCTCT TATACCCCGA AGAGCCCAGT CACCTGAACA GAACACAGGC
    AAACAGAAAG CCAAAAGTCA AGCCAAGGCA AAGGAGGAGC CCCCCACAGA CCACCTTGGG
    CAGATCTCCG AGGCTGATGA AAAACTTATC ACTGACACCT GGCAAACTGC TGTTTCAGCC
    ATTGCAAACA TGGTGTCGAA TGAAAAACAG TCCAAAGAAG CAGAAGAGGA AAAAGAAAGG
    CAACTGATGG AAATGAAGGA AAAAGAGAGA GCGAAAGCTT CACAAGCTGC ATCTCGGGCT
    AGTGGCAAAG GAGGGAAAAA GAAAGCGCCG CCAAAGTCGC CAAACAGGAA AAAAGAAGCA
    AAGTCCCCAG GCCCTGCAGA CCCCACACCT TCTCTTCAGA CCCTAGAAGA CAGTACTGAG
    TTGCAAAAGA AGCAGGAGCT AAAGATGAAG TTGAAGCAGG AGCATATTGC TGCTTTAGAG
    TATGAAGAGA TGGCTGCTAA CTTACGGCTG GAAGTTATTA AAACAAAAGC CTTAGAAGTG
    TGCCAGGATA TTGCATCGAG AGCTGAGAGT GCCTACAAAG ATATGGAAAT GTGGATGGGA
    GCAAGATTCC TGGCAGAAAT GGCAAGCGTG GAGCAACTCA TCCATATTGC TCGTCACCAT
    ATTGAAACGG GCACCAAAAT AAAGTATCAG CTTGTCCTGG AGAATACAGA TTTCTACATT
    AATTCAGATG TACAAGTGGT GCCCGATCCT CCGCCACCTT CTCGCCCTCC TGCAGTAGAG
    ATATCAGCCA ATGCGACACT GACTGTCGCT CAGCTCCACA GATTATACCA GCAGTTTGCT
    CAGATCGCTC CTGAAGGACT AATATCCAAT AAAACGTTTA TCGATATTCT CCTGGATATT
    ACATCAGCGA GACTTGGAAA CGATGCCCTC CCTGATTCGT GGATGCAGCT TACACTTCCA
    GAGATAAAAG AAATGACATC TGAATTATCT CAGAACTCTG ATGTGGTAAA CTGGCGCCTA
    TTCCTGCTGT CAGCATCTCT GCCATGGCCG TACCCCTCCG TAACGCAGCT TCTCAGCACT
    CTGCAGAGCT TCAAGGCTGT GGATCATAAT GGCTGCGGCA CACTGACCCA CGAAGGTTTC
    AGTCAGGCTG AGCTGTGGTT TCATGGAAAT GCAGAGGAAC CTGTCCCTGA AAATCCCACA
    GAGCCTTTGC CATTTAACAG ACCGGCCCAT CTAATAAAGT ATTTCTTTGA CTTGTTTGCT
    GACCCCCGGG GAATCCCCCC CCAGCTGAAT TACACAGAGA TGCTGCTGTA TTTTGCCTCA
    CACCCCGACC CTATGGAAGG CTTCTATCGG GCGCTGAGTG TTACGGCGGG GAAGCCTATT
    CTGAAGGCAC CAGGAAATAA TGTTTTGCTT GAGTCTCTCC CAGCAATGGA GGCCATTCAG
    CAGATGAAAG AACCAATCAG CAGCAATGAC ACAGTGGGGA CACGAGGCTT GGGAAAGGCA
    GCGGTCACAT TACATGAAGT GGTGAGAGTG CTGCAGCACG CTGCTGGGAC GGGTGAAGAT
    AACCATAGGT TTTGCCTGGA AGAAAATGGC AGGAACAATA TTTATAAGGT ACTTTTACCT
    TTGTCTCATG AAAACAAGAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946318.1
    CDS229..5232
    Translation

    Target ORF information:

    RefSeq Version XM_018090829.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCAGAAC AGCACGGAGT GGCTACCCGG CTTCTGTATC AGTTGTTCAT AGCATTGCAG 
    AAGAAAAAGA AGGCAGGATT GACAGGAGTA GCCATGGAGA CATTGAGACC AGCTGCTCCA
    GCAAAGCTTC AGAGCATTGG AACTGAGATG TACAGAGAGC GTTTGAAAAC CATAATCCCC
    CGCCAAAGTG AAATCAGCCT GCAGAGGGTG TCAGAACAAT TTGACCTGAA AGCAAAAGAA
    ATCGAAGACA AAGTTGCACA GATTCAAAAT GATGAACTTC GGAAAATACA GAAAATACAA
    GAAGAGCTAA GAATTCAGGA TATTGAAAAG CTGCGACGAG CTAGAAGGAG ACAAACCGAA
    ATAATGGCAC GAATTCAAGC AGCAATTGTT CAGATTCCAA AACCACCTCA AAACCGCACA
    TTGAAGGCCA TCGAAGCACA AAAGATGCTG AAAAAGAAGA AAGAAGCTGA GGAAGTTTAT
    ACAGAAATCA CAAAGTTTGA AAAAGGAGTG AAAGATGGAT CTGTTGGAAG CTTGTCTGCT
    AATACCAGGG GCCCAACACT CCAGAGATTA AGGTCATCAG TTAACATTGG CACTGCTTTG
    CTAAAACTGG ATACTAATGA TGATTACGTA AGGAAAATTC AGAAGCGATT AGAGGAGGAT
    TCTGTAGCAC GTGAGCAGCG CGAAAAGAGA CGTCGGCGGA TTCTTATGGA ACAGCTTGTG
    GGTCATGGGG CACAAGAGGA AGCTTTCCGG GAGGAACAAC TGATAAACCG ATTAATGAGA
    CAGTCCCAGC AAGAGCGGAG AATTGCAGTT CAGCTTATGC ACGCGCGCCA TGAAAAGGAA
    GTTATGCAAC AGAACCGAAT GTTCCGGGAG AAGCAATATC AGGAGAGGCG CGAGAGGGAG
    TTCCAAGAAG CTCTTGACAG AGAAGCGGCG TTGCAGAAAC AAGCGATGCT CGACCACAAA
    GAAGAAATAC GCAAAGTGCA GGAGCTCCAT GACCAGATAG CAGCGGAGCG TGCAGAGGCT
    CGCTATAAAA AGCACTATAA CCTGTGCCAG GAAATCATGA ATCAGATTTT AGACTTTGTT
    ATTAAAACAG GGGAATACAG AGAGCTAACA AACAGACTTA TACCTGTTAA ATTGATGCGG
    GAGTGGAAAG AACTATTCTT CGCTGGTTAC CCGCTTTACG ACAAAGCACC TATAGATGCC
    CTCCCTTCCG ACCCAACTGC AGAGCAGCTG CAAGAAACAG AAAAGCAGAG TCTGTTAGAT
    GAGAAGGATT ATGAAGAATA CAAGACCATG ACAGGGGAAT GGGGCCCACC CGAAGCCAGC
    AGTGTTAAAG CACCTGCACC GAACAACAAC ATATTGGGCT ATGTGGTTCA TAGACTGTTG
    GAAATTGTCA GCCCCCCATT GGCACCCACA CCGCCACCTG TGTTTCCCCC CTTCCCCATC
    AAGGGTTGTG TTCTTGGGAA GATTTACTCT GGGAAGAGCA CGTGCCTGAA ACATCTGGCA
    CAGGTGTATA GCATCCAAGT TCTCTCTGTG GACACCCTTC TGGAGGAAGC TATTCAAGCA
    TTTAATGACG GGGAACTGGA AAAGACCGAG GATGTTCTGA GGCCCATACA AGAAGGAGCA
    GATAAAGAGA ACAAGGTACC CATAGACAGT CCTGCTCCTG TTACTAATGG CGAAGAGATA
    CCTGTTGTGG GAAGTGGCAG CAAGCCTGCA TCTGCAGAAG CCCGTGAATC AGATAAAGAG
    TTATCTCTAC ATCAAATATT AAAGCCTGCA ATAGAAGATA CTGTGCCAAA GCTCTCTGCA
    CGGGCACAGC TTGGTGGACT GGCAGAGACA CATTTAAAGA GTGGGAAGAG TGTCCCAGAT
    GAGCTGCTTA TCAGTATACT TGTTGAAGCC ATCAATCGCA TCCCAGCTAA CACAGGGTGG
    ATCATGGAGG GATTCCCTAT GACGGAAACT CAGGCCACAT TATTTGAAAA GGCTCTCACT
    GGCTCACACA CTAATTTGAA TGGAATAAAG GGGAATAATA ACAAGTTTGC CTCTTTAGTA
    ACAGATCCAG CAGCACCAAA GGATCTCCCT GTGGCTCCAT CTGCTCTTGA CTTTGCTGTG
    TTAATAGAAA TATCAGACAA TGAAGTGTTA CAGCGCATGT CTACAATAAT CAGTGAAATA
    GATAAATCTG GAGAGAAAAT TGATAGTCCT CCCTGTATTC AAACTACGAG AATGGAAGCA
    GATCAAACAA TGGACCAGAT ACAGCACAGA ATTACAGGAT TTCTTGACCA CTGGAAGAAT
    TTAGAAATGT GGTTTTCAGA GCAGCAGAAT ATTCTAATCA AAGTTAATGG GGAAGTGGAA
    GTTGGCGTTC TGTGCAAGAG AATAGAAGAA GTATACTTGA CAGCTCTCGT CAATAAGCAA
    AACCGAGGCA AAAAAAACGA AAAGAAAGAA GATCTCCCAC CACCAGAAGT AGCCGCACCA
    ACACCGCCAC CGCCATCTCC TCCTCCAGCT CCAGAAGCCA CTAACAATTT ACGACCTCCA
    TCAGCTTCAA GCAAGGAATC AGCAAAAAAA AGCAGGAAAG GCTCCAAATC TCCAAGAGAA
    TCTCGAAAAC AGTCTGACAC CTCGAAGGAC AAGAAGGACA AGAAACCAGA GACACCTAAA
    GGAAAAGACT CTCGAAAATC TGGTTCACCA CGTGGAAGGT CTCCTGGAAA GAAGGCTAAG
    TCTGTACCTG CCAGCCCTGA ACCAGTCGCT GTCACTCCTG TTGGACCACC ACCCATCCAG
    CCTGGATCCC CTGAATGGGT GTACGTAAAT GAGCCACTTC CGAAAGAAAT CCCAGCATTC
    CTTGTACCAT ATTGGAAGAC TATAGAAAAT GTCTATGGAA TGACCATCAA AACTGTGCTG
    AGGAATCTGA GGCAGGAACG ACTAACTGTG ATTCACTATT TATATGATAT CAGGGTTCAT
    TTGAAGGATT ACTTGAAGCG GCCGGATCAC AAGCAGGAAT TCGTGTCTCA GTGGCAGTCG
    GACTTTAATT CTACCGCAGA CGACTTGTGG GAGGACGAGG AAACAAAAGC AGAGCTTCAT
    CAAAGAGCTG ATGATCTCCG GGACCGTCTG TGGGACATAT GTGACAACAG GAAGGAGGAG
    GCAGAACAAG AGCGCACAGA CATTATGAAT GATGGGTGGC TACAGGATCA CACCGGGATA
    CTTATTAACC ACTTTTTCTC TCTGATGCAG GCGGAAGTTG ATCGCTTCCA AGACACCACA
    AGACTTCTCA GTGATTACTA CCAAGGAATG GAGGGACAAG CCCCATCTGA GGCCAGCCAT
    ACCTTTTCCC GTATCCCACT GCTGGACATT GCTAACTCAA GCACGCAGGG GGATTCTGAG
    AACCCTAAAA GAATTCCTCT TATACCCCGA AGAGCCCAGT CACCTGAACA GAACACAGGC
    AAACAGAAAG CCAAAAGTCA AGCCAAGGCA AAGGAGGAGC CCCCCACAGA CCACCTTGGG
    CAGATCTCCG AGGCTGATGA AAAACTTATC ACTGACACCT GGCAAACTGC TGTTTCAGCC
    ATTGCAAACA TGGTGTCGAA TGAAAAACAG TCCAAAGAAG CAGAAGAGGA AAAAGAAAGG
    CAACTGATGG AAATGAAGGA AAAAGAGAGA GCGAAAGCTT CACAAGCTGC ATCTCGGGCT
    AGTGGCAAAG GAGGGAAAAA GAAAGCGCCG CCAAAGTCGC CAAACAGGAA AAAAGAAGCA
    AAGTCCCCAG GCCCTGCAGA CCCCACACCT TCTCTTCAGA CCCTAGAAGA CAGTACTGAG
    TTGCAAAAGA AGCAGGAGCT AAAGATGAAG TTGAAGCAGG AGCATATTGC TGCTTTAGAG
    TATGAAGAGA TGGCTGCTAA CTTACGGCTG GAAGTTATTA AAACAAAAGC CTTAGAAGTG
    TGCCAGGATA TTGCATCGAG AGCTGAGAGT GCCTACAAAG ATATGGAAAT GTGGATGGGA
    GCAAGATTCC TGGCAGAAAT GGCAAGCGTG GAGCAACTCA TCCATATTGC TCGTCACCAT
    ATTGAAACGG GCACCAAAAT AAAGTATCAG CTTGTCCTGG AGAATACAGA TTTCTACATT
    AATTCAGATG TACAAGTGGT GCCCGATCCT CCGCCACCTT CTCGCCCTCC TGCAGTAGAG
    ATATCAGCCA ATGCGACACT GACTGTCGCT CAGCTCCACA GATTATACCA GCAGTTTGCT
    CAGATCGCTC CTGAAGGACT AATATCCAAT AAAACGTTTA TCGATATTCT CCTGGATATT
    ACATCAGCGA GACTTGGAAA CGATGCCCTC CCTGATTCGT GGATGCAGCT TACACTTCCA
    GAGATAAAAG AAATGACATC TGAATTATCT CAGAACTCTG ATGTGGTAAA CTGGCGCCTA
    TTCCTGCTGT CAGCATCTCT GCCATGGCCG TACCCCTCCG TAACGCAGCT TCTCAGCACT
    CTGCAGAGCT TCAAGGCTGT GGATCATAAT GGCTGCGGCA CACTGACCCA CGAAGGTTTC
    AGTCAGGCTG AGCTGTGGTT TCATGGAAAT GCAGAGGAAC CTGTCCCTGA AAATCCCACA
    GAGCCTTTGC CATTTAACAG ACCGGCCCAT CTAATAAAGT ATTTCTTTGA CTTGTTTGCT
    GACCCCCGGG GAATCCCCCC CCAGCTGAAT TACACAGAGA TGCTGCTGTA TTTTGCCTCA
    CACCCCGACC CTATGGAAGG CTTCTATCGG GCGCTGAGTG TTACGGCGGG GAAGCCTATT
    CTGAAGGCAC CAGGAAATAA TGTTTTGCTT GAGTCTCTCC CAGCAATGGA GGCCATTCAG
    CAGATGAAAG AACCAATCAG CAGCAATGAC ACAGTGGGGA CACGAGGCTT GGGAAAGGCA
    GCGGTCACAT TACATGAAGT GGTGAGAGTG CTGCAGCACG CTGCTGGGAC GGGTGAAGAT
    AACCATAGGT TTTGCCTGGA AGAAAATGGC AGGAACAATA TTTATAAGGT ACTTTTACCT
    TTGTCTCATG AAAACAAGAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49220
    Clone ID Related Accession (Same CDS sequence) XM_031894698.1
    Accession Version XM_031894698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTA
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGAGCCTCG GGCAGATATT TGCGGAGCTG GGCACCGAGC AGCAGGAATG TGTAGCTGTC
    GCAGCTCTTA TGAGCCACCC TGCATTCCAA GACCTTCTCA GCAACTGTCA CATCTACAAA
    TTCCCAGATA TTCATAGAAT TCTAAACAAG CTGAAAGCCC CCCAGGCGAG TGATGGAGAG
    ACTTTGCTGC CTGTGAGTTC AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750558.1
    CDS129..5555
    Translation

    Target ORF information:

    RefSeq Version XM_031894698.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA
    GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT
    ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA
    AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG
    GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT
    ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT
    ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC
    CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA
    TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT
    ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG
    CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG
    GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG
    CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTA
    ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC
    CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC
    TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG
    CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG
    GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA
    ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA
    GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT
    GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT
    AAGAGCCTCG GGCAGATATT TGCGGAGCTG GGCACCGAGC AGCAGGAATG TGTAGCTGTC
    GCAGCTCTTA TGAGCCACCC TGCATTCCAA GACCTTCTCA GCAACTGTCA CATCTACAAA
    TTCCCAGATA TTCATAGAAT TCTAAACAAG CTGAAAGCCC CCCAGGCGAG TGATGGAGAG
    ACTTTGCTGC CTGTGAGTTC AAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49221
    Clone ID Related Accession (Same CDS sequence) XM_031894702.1
    Accession Version XM_031894702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT
    GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA
    TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA
    AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG
    AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT
    AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA
    AAGGCTCTCA CTGGCTCACA CACTGATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT
    GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT
    GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA
    ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG
    AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC
    CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT
    GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC
    GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA
    GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT
    TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA
    TCTCCAAGAG AATCTCGAAA ACGGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA
    GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA
    AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA
    CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA
    ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC
    AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT
    ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT
    CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA
    GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC
    AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT
    CACACCGGGA TACTTATTAA CCACTTTTTC TCACTGATGC AGGCGGAAGT TGATCGCTTC
    CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT
    GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG
    GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA
    CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA
    GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT
    GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG
    GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT
    GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG
    AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA
    GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC
    CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT
    TTGCTGCCTG TGAGTTCAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750562.1
    CDS129..5552
    Translation

    Target ORF information:

    RefSeq Version XM_031894702.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT
    GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA
    TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA
    AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG
    AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT
    AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA
    AAGGCTCTCA CTGGCTCACA CACTGATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT
    GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT
    GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA
    ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG
    AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC
    CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT
    GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC
    GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA
    GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT
    TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA
    TCTCCAAGAG AATCTCGAAA ACGGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA
    GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA
    AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA
    CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA
    ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC
    AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT
    ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT
    CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA
    GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC
    AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT
    CACACCGGGA TACTTATTAA CCACTTTTTC TCACTGATGC AGGCGGAAGT TGATCGCTTC
    CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT
    GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG
    GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA
    CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA
    GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT
    GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG
    GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT
    GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG
    AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA
    GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC
    CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT
    TTGCTGCCTG TGAGTTCAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49222
    Clone ID Related Accession (Same CDS sequence) XM_031894707.1
    Accession Version XM_031894707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5424bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA
    GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC
    CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT
    TTGCTGCCTG TGAGTTCAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750567.1
    CDS129..5552
    Translation

    Target ORF information:

    RefSeq Version XM_031894707.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
    CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA
    TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA
    AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT
    GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG
    TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG
    AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG
    AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC
    CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA
    ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA
    AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA
    CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA
    GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT
    GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC
    ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG
    CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT
    ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA
    AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG
    CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG
    AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG
    ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG
    GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG
    ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT
    GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA
    GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG
    CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC
    CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG
    GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT
    CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC
    CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG
    GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC
    ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT
    AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT
    GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG
    CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG
    AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA
    GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT
    GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG
    TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT
    CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA
    ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT
    ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT
    GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT
    AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT
    CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA
    GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC
    AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC
    AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA
    CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT
    GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA
    CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA
    GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC
    ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT
    GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG
    TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA
    AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC
    AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG
    GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC
    TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA
    TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG
    CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT
    GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC
    ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA
    ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA
    GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA
    GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC
    AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA
    GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT
    GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA
    GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA
    ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT
    GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA
    GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT
    CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC
    CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT
    CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG
    CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA
    AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG
    CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC
    CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT
    GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT
    GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG
    TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG
    GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG
    GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC
    TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG
    ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG
    AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA
    GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC
    CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT
    TTGCTGCCTG TGAGTTCAAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.