smurf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smurf1
Entrez Gene ID	100491582
Full Name	SMAD specific E3 ubiquitin protein ligase 1
Synonyms	xSMURF1
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931494.5	XP_002931540.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_004917842.3	XP_004917899.1	E3 ubiquitin-protein ligase SMURF1 isoform X2
XM_031892958.1	XP_031748818.1	E3 ubiquitin-protein ligase SMURF1 isoform X3
XM_002931494.4	XP_002931540.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
XM_004917842.4	XP_004917899.1	E3 ubiquitin-protein ligase SMURF1 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Gene Ontology

The following smurf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smurf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16119	XM_002931494.4 Latest version!	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa16120	XM_004917842.3 Latest version!	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa66390	XM_002931494.5 Latest version!	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66391	XM_004917842.4 Latest version!	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66392	XM_031892958.1 Latest version!	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smurf1 ( XM_002931494.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931540.1 Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16119
Clone ID Related Accession (Same CDS sequence)	XM_002931494.4
Accession Version	XM_002931494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT 
TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT 
AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA 
GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931540.1
CDS	436..2631
Translation	MSNAVTRRGGSSIRVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIINHQSQLKEPNNAIPVQSDGSLEEGDEFPTQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE

Target ORF information:

RefSeq Version	XM_002931494.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931494.4

ORF Insert Sequence:

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT 
TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT 
AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA 
GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_004917842.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917899.1 Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16120
Clone ID Related Accession (Same CDS sequence)	XM_004917842.3
Accession Version	XM_004917842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT 
CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT 
GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA 
GAGACTAGTG GTTTTGCTGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917899.1
CDS	438..2624
Translation	MSNAVTRRGGSSIRVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIINHQSQLKEPNNAIPVQSDGSLEEGDEFPTQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE

Target ORF information:

RefSeq Version	XM_004917842.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917842.3

ORF Insert Sequence:

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT 
CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT 
GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA 
GAGACTAGTG GTTTTGCTGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_002931494.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931540.1 Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66390
Clone ID Related Accession (Same CDS sequence)	XM_002931494.5
Accession Version	XM_002931494.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931494.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT 
TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT 
AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA 
GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931540.1
CDS	149..2344
Translation	MSNAVTRRGGSSIRVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIINHQSQLKEPNNAIPVQSDGSLEEGDEFPTQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE

Target ORF information:

RefSeq Version	XM_002931494.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931494.5

ORF Insert Sequence:

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT 
TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT 
AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA 
GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_004917842.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917899.1 Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

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CloneID	OXa66391
Clone ID Related Accession (Same CDS sequence)	XM_004917842.4
Accession Version	XM_004917842.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917842.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT 
CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT 
GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA 
GAGACTAGTG GTTTTGCTGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917899.1
CDS	150..2336
Translation	MSNAVTRRGGSSIRVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIINHQSQLKEPNNAIPVQSDGSLEEGDEFPTQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE

Target ORF information:

RefSeq Version	XM_004917842.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917842.4

ORF Insert Sequence:

ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA 
GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA 
AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC 
TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA 
TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA 
TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA 
GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA 
GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC 
CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA 
GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC 
CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA 
CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA 
TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT 
TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC 
AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC 
CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG 
GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA 
CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA 
GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA 
AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG 
TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT 
ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA 
TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT 
AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT 
GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC 
ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC 
CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG 
TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT 
CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA 
GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC 
ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG 
GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT 
GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT 
CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT 
GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA 
GAGACTAGTG GTTTTGCTGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smurf1 ( XM_031892958.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748818.1 Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X3, mRNA.

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CloneID	OXa66392
Clone ID Related Accession (Same CDS sequence)	XM_031892958.1
Accession Version	XM_031892958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTTT TATGTGCGAA GAACCTAGCA AAGAGGGATT TTTTCAGATT ACCTGACCCA 
TTTGCTAAAA TAGTTGTGGA TGGATCTGGT CAGTGCCACT CAACTGACAC TGTAAAGAAC 
ACATTAGACC CAAAGTGGAA TCAGCATTAT GACCTATATG TTGGTAAAAT GGATTCAATT 
ACAATCAGTA TCTGGAATCA TAAGAAAATT CACAAGAAAC AGGGAGCTGG CTTCCTGGGG 
TGTGTGAGAT TATTATCCAA TGCCATTAGC AGATTGAAAG ACACAGGCTA CCAGCGCTTG 
GATCTATGCA AATTAAATCC CGCAGATAGT GATGCTGTAC GGGGCCAAAT TGTAGTCAGT 
TTGCAGACAC GAGACAGGAT CGGTACAGTT GGCTCTGTTG TTGATTGCAG AGGACTGCTA 
GACAATGATG GAGCACTTTA TGAAGATGCT GGGCCTGGCA GGCCCCTTAG TTGTTTCATG 
GATGAGCCAG CCCCTTATAC TGATGGACCA GGTGCAGCTG GAGGAGGGCC CTGTAGGCTA 
GTAGAATCGC CAGGTCAAGA ACAGAGACTC CAAGCACAGA GACTTCGTGG ACCAGAAGTT 
AGAGAGCATG TCCAGACACC TTTAAATCGA TCCCATGGGT ACCAGTCACA GGACTTGCCT 
GAAGGTTATG AACAAAGAAC TACGGTTCAA GGGCAGGTGT ATTTCTTGCA CACACAAACT 
GGTGTCAGCA CATGGCATGA TCCCCGAATA CCAAGAGACC TTAACAGTGT GAATTGTGAT 
GACCTTGGCC CTTTGCCCAC TGGATGGGAA GTCAGAACCA CTGTGTCTGG AAGAATATAC 
TTTGTAGACC ACAATAATCG AACAACGCAG TTCACAGACC CAAGACTACA TCACATCATA 
AATCATCAGT CCCAGCTAAA AGAGCCAAAT AATGCTATAC CAGTGCAGAG TGATGGATCT 
TTAGAAGAGG GGGATGAATT TCCTACTCAA AGATATGAGA GAGACTTGGT ACAGAAACTT 
AAAGTCCTGA GACATGAGTT ATCATTGCTG CAGCCTCAGG CAGGGCACTG CCGTGTTGAA 
GTGTCAAGGG AAGAAATTTT TGAGGAGTCC TATAGGCAGA TAATGAAGAT GAGACCGAAA 
GACCTTAAGA AAAGATTGAT GGTGAAATTT CGTGGGGAAG AAGGACTTGA CTATGGTGGA 
GTGGCCAGGG AGTGGCTGTA TTTACTATGC CATGAAATGC TGAATCCATA TTATGGACTT 
TTCCAATATT CTACAGACAA TATTTATACT CTGCAAATTA ATCCTGATTC ATCTATTAAT 
CCAGATCATT TATCTTATTT CCATTTTGTT GGTCGAATCA TGGGATTGGC TGTGTTTCAT 
GGACACTACA TTAATGGGGG GTTTACAGTT CCTTTCTACA AACAACTTTT GGGAAAGCCC 
ATTCAGCTGT CTGACTTGGA ATCTGTGGAT CCAGAGCTGC ATAAAAGCTT GGTTTGGATT 
TTAGAGAATG ACATTACTTC AGTATTAGAC CACACGTTCT GTGTTGAGCA CAATGCATTT 
GGCCGACTCC TCCAGCATGA ATTGAAACCA AATGGCAAGA ACTTACAAGT AACAGAAGAA 
AATAAGAAAG AGTATGTGAG GTTGTATGTT AATTGGAGGT TCATGAGAGG GATAGAGGCT 
CAGTTCTTGG CTCTGCAGAA AGGTTTTAAT GAGCTAATAC CACAACATTT GTTGAAGCCA 
TTTGAGCAAA AAGAACTAGA GCTTATTATT GGAGGCTTGG ATAAAATTGA CATCAGTGAC 
TGGAAGGCAA ACACTAGACT AAAACACTGC TTAGCTAATA GCAATATTGT TCAGTGGTTC 
TGGCAAGCAG TGGAGTCGTT TGATGAGGAA AGAAGGGCCA GACTTCTGCA GTTTGTCACG 
GGCTCTACGC GTGTTCCACT TCAGGGCTTT AAAGCTCTTC AAGGTTCTAC AGGTGCTGCT 
GGACCCAGAC TTTTTACTAT TCATCTGATT GATGCCAACA CAGATAACCT TCCAAAGGCT 
CACACGTGCT TTAACCGAAT TGACATTCCT CCTTATGAAT CCTATGAAAA GCTCTATGAG 
AAATTATTAA CAGCTGTAGA AGAGACTAGT GGTTTTGCTG TGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748818.1
CDS	16..2163
Translation	MTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPADSDAVRGQIVVSLQTRDRIGTVGSVVDCRGLLDNDGALYEDAGPGRPLSCFMDEPAPYTDGPGAAGGGPCRLVESPGQEQRLQAQRLRGPEVREHVQTPLNRSHGYQSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGPLPTGWEVRTTVSGRIYFVDHNNRTTQFTDPRLHHIINHQSQLKEPNNAIPVQSDGSLEEGDEFPTQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE

Target ORF information:

RefSeq Version	XM_031892958.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892958.1

ORF Insert Sequence:

ATGACAGTTT TATGTGCGAA GAACCTAGCA AAGAGGGATT TTTTCAGATT ACCTGACCCA 
TTTGCTAAAA TAGTTGTGGA TGGATCTGGT CAGTGCCACT CAACTGACAC TGTAAAGAAC 
ACATTAGACC CAAAGTGGAA TCAGCATTAT GACCTATATG TTGGTAAAAT GGATTCAATT 
ACAATCAGTA TCTGGAATCA TAAGAAAATT CACAAGAAAC AGGGAGCTGG CTTCCTGGGG 
TGTGTGAGAT TATTATCCAA TGCCATTAGC AGATTGAAAG ACACAGGCTA CCAGCGCTTG 
GATCTATGCA AATTAAATCC CGCAGATAGT GATGCTGTAC GGGGCCAAAT TGTAGTCAGT 
TTGCAGACAC GAGACAGGAT CGGTACAGTT GGCTCTGTTG TTGATTGCAG AGGACTGCTA 
GACAATGATG GAGCACTTTA TGAAGATGCT GGGCCTGGCA GGCCCCTTAG TTGTTTCATG 
GATGAGCCAG CCCCTTATAC TGATGGACCA GGTGCAGCTG GAGGAGGGCC CTGTAGGCTA 
GTAGAATCGC CAGGTCAAGA ACAGAGACTC CAAGCACAGA GACTTCGTGG ACCAGAAGTT 
AGAGAGCATG TCCAGACACC TTTAAATCGA TCCCATGGGT ACCAGTCACA GGACTTGCCT 
GAAGGTTATG AACAAAGAAC TACGGTTCAA GGGCAGGTGT ATTTCTTGCA CACACAAACT 
GGTGTCAGCA CATGGCATGA TCCCCGAATA CCAAGAGACC TTAACAGTGT GAATTGTGAT 
GACCTTGGCC CTTTGCCCAC TGGATGGGAA GTCAGAACCA CTGTGTCTGG AAGAATATAC 
TTTGTAGACC ACAATAATCG AACAACGCAG TTCACAGACC CAAGACTACA TCACATCATA 
AATCATCAGT CCCAGCTAAA AGAGCCAAAT AATGCTATAC CAGTGCAGAG TGATGGATCT 
TTAGAAGAGG GGGATGAATT TCCTACTCAA AGATATGAGA GAGACTTGGT ACAGAAACTT 
AAAGTCCTGA GACATGAGTT ATCATTGCTG CAGCCTCAGG CAGGGCACTG CCGTGTTGAA 
GTGTCAAGGG AAGAAATTTT TGAGGAGTCC TATAGGCAGA TAATGAAGAT GAGACCGAAA 
GACCTTAAGA AAAGATTGAT GGTGAAATTT CGTGGGGAAG AAGGACTTGA CTATGGTGGA 
GTGGCCAGGG AGTGGCTGTA TTTACTATGC CATGAAATGC TGAATCCATA TTATGGACTT 
TTCCAATATT CTACAGACAA TATTTATACT CTGCAAATTA ATCCTGATTC ATCTATTAAT 
CCAGATCATT TATCTTATTT CCATTTTGTT GGTCGAATCA TGGGATTGGC TGTGTTTCAT 
GGACACTACA TTAATGGGGG GTTTACAGTT CCTTTCTACA AACAACTTTT GGGAAAGCCC 
ATTCAGCTGT CTGACTTGGA ATCTGTGGAT CCAGAGCTGC ATAAAAGCTT GGTTTGGATT 
TTAGAGAATG ACATTACTTC AGTATTAGAC CACACGTTCT GTGTTGAGCA CAATGCATTT 
GGCCGACTCC TCCAGCATGA ATTGAAACCA AATGGCAAGA ACTTACAAGT AACAGAAGAA 
AATAAGAAAG AGTATGTGAG GTTGTATGTT AATTGGAGGT TCATGAGAGG GATAGAGGCT 
CAGTTCTTGG CTCTGCAGAA AGGTTTTAAT GAGCTAATAC CACAACATTT GTTGAAGCCA 
TTTGAGCAAA AAGAACTAGA GCTTATTATT GGAGGCTTGG ATAAAATTGA CATCAGTGAC 
TGGAAGGCAA ACACTAGACT AAAACACTGC TTAGCTAATA GCAATATTGT TCAGTGGTTC 
TGGCAAGCAG TGGAGTCGTT TGATGAGGAA AGAAGGGCCA GACTTCTGCA GTTTGTCACG 
GGCTCTACGC GTGTTCCACT TCAGGGCTTT AAAGCTCTTC AAGGTTCTAC AGGTGCTGCT 
GGACCCAGAC TTTTTACTAT TCATCTGATT GATGCCAACA CAGATAACCT TCCAAAGGCT 
CACACGTGCT TTAACCGAAT TGACATTCCT CCTTATGAAT CCTATGAAAA GCTCTATGAG 
AAATTATTAA CAGCTGTAGA AGAGACTAGT GGTTTTGCTG TGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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smurf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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