smurf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following smurf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smurf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa16119 XM_002931494.4
Latest version!
Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa16120 XM_004917842.3
Latest version!
Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa66390 XM_002931494.5
Latest version!
Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa66391 XM_004917842.4
Latest version!
Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa66392 XM_031892958.1
Latest version!
Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa16119
    Clone ID Related Accession (Same CDS sequence) XM_002931494.4
    Accession Version XM_002931494.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT
    TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT
    AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA
    GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931540.1
    CDS436..2631
    Translation

    Target ORF information:

    RefSeq Version XM_002931494.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931494.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT
    TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT
    AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA
    GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16120
    Clone ID Related Accession (Same CDS sequence) XM_004917842.3
    Accession Version XM_004917842.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT
    CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT
    GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA
    GAGACTAGTG GTTTTGCTGT GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917899.1
    CDS438..2624
    Translation

    Target ORF information:

    RefSeq Version XM_004917842.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917842.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CGTTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT
    CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT
    GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA
    GAGACTAGTG GTTTTGCTGT GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66390
    Clone ID Related Accession (Same CDS sequence) XM_002931494.5
    Accession Version XM_002931494.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931494.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT
    TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT
    AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA
    GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931540.1
    CDS149..2344
    Translation

    Target ORF information:

    RefSeq Version XM_002931494.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931494.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTTCTACAG GTGCTGCTGG ACCCAGACTT
    TTTACTATTC ATCTGATTGA TGCCAACACA GATAACCTTC CAAAGGCTCA CACGTGCTTT
    AACCGAATTG ACATTCCTCC TTATGAATCC TATGAAAAGC TCTATGAGAA ATTATTAACA
    GCTGTAGAAG AGACTAGTGG TTTTGCTGTG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66391
    Clone ID Related Accession (Same CDS sequence) XM_004917842.4
    Accession Version XM_004917842.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917842.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT
    CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT
    GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA
    GAGACTAGTG GTTTTGCTGT GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917899.1
    CDS150..2336
    Translation

    Target ORF information:

    RefSeq Version XM_004917842.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917842.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAACG CTGTGACTCG GAGAGGTGGA TCGAGTATCA GAGTTCGTCT GACAGTTTTA 
    TGTGCGAAGA ACCTAGCAAA GAGGGATTTT TTCAGATTAC CTGACCCATT TGCTAAAATA
    GTTGTGGATG GATCTGGTCA GTGCCACTCA ACTGACACTG TAAAGAACAC ATTAGACCCA
    AAGTGGAATC AGCATTATGA CCTATATGTT GGTAAAATGG ATTCAATTAC AATCAGTATC
    TGGAATCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGGTG TGTGAGATTA
    TTATCCAATG CCATTAGCAG ATTGAAAGAC ACAGGCTACC AGCGCTTGGA TCTATGCAAA
    TTAAATCCCG CAGATAGTGA TGCTGTACGG GGCCAAATTG TAGTCAGTTT GCAGACACGA
    GACAGGATCG GTACAGTTGG CTCTGTTGTT GATTGCAGAG GACTGCTAGA CAATGATGGA
    GCACTTTATG AAGATGCTGG GCCTGGCAGG CCCCTTAGTT GTTTCATGGA TGAGCCAGCC
    CCTTATACTG ATGGACCAGG TGCAGCTGGA GGAGGGCCCT GTAGGCTAGT AGAATCGCCA
    GGTCAAGAAC AGAGACTCCA AGCACAGAGA CTTCGTGGAC CAGAAGTTAG AGAGCATGTC
    CAGACACCTT TAAATCGATC CCATGGGTAC CAGTCACAGG ACTTGCCTGA AGGTTATGAA
    CAAAGAACTA CGGTTCAAGG GCAGGTGTAT TTCTTGCACA CACAAACTGG TGTCAGCACA
    TGGCATGATC CCCGAATACC AAGAGACCTT AACAGTGTGA ATTGTGATGA CCTTGGCCCT
    TTGCCCACTG GATGGGAAGT CAGAACCACT GTGTCTGGAA GAATATACTT TGTAGACCAC
    AATAATCGAA CAACGCAGTT CACAGACCCA AGACTACATC ACATCATAAA TCATCAGTCC
    CAGCTAAAAG AGCCAAATAA TGCTATACCA GTGCAGAGTG ATGGATCTTT AGAAGAGGGG
    GATGAATTTC CTACTCAAAG ATATGAGAGA GACTTGGTAC AGAAACTTAA AGTCCTGAGA
    CATGAGTTAT CATTGCTGCA GCCTCAGGCA GGGCACTGCC GTGTTGAAGT GTCAAGGGAA
    GAAATTTTTG AGGAGTCCTA TAGGCAGATA ATGAAGATGA GACCGAAAGA CCTTAAGAAA
    AGATTGATGG TGAAATTTCG TGGGGAAGAA GGACTTGACT ATGGTGGAGT GGCCAGGGAG
    TGGCTGTATT TACTATGCCA TGAAATGCTG AATCCATATT ATGGACTTTT CCAATATTCT
    ACAGACAATA TTTATACTCT GCAAATTAAT CCTGATTCAT CTATTAATCC AGATCATTTA
    TCTTATTTCC ATTTTGTTGG TCGAATCATG GGATTGGCTG TGTTTCATGG ACACTACATT
    AATGGGGGGT TTACAGTTCC TTTCTACAAA CAACTTTTGG GAAAGCCCAT TCAGCTGTCT
    GACTTGGAAT CTGTGGATCC AGAGCTGCAT AAAAGCTTGG TTTGGATTTT AGAGAATGAC
    ATTACTTCAG TATTAGACCA CACGTTCTGT GTTGAGCACA ATGCATTTGG CCGACTCCTC
    CAGCATGAAT TGAAACCAAA TGGCAAGAAC TTACAAGTAA CAGAAGAAAA TAAGAAAGAG
    TATGTGAGGT TGTATGTTAA TTGGAGGTTC ATGAGAGGGA TAGAGGCTCA GTTCTTGGCT
    CTGCAGAAAG GTTTTAATGA GCTAATACCA CAACATTTGT TGAAGCCATT TGAGCAAAAA
    GAACTAGAGC TTATTATTGG AGGCTTGGAT AAAATTGACA TCAGTGACTG GAAGGCAAAC
    ACTAGACTAA AACACTGCTT AGCTAATAGC AATATTGTTC AGTGGTTCTG GCAAGCAGTG
    GAGTCGTTTG ATGAGGAAAG AAGGGCCAGA CTTCTGCAGT TTGTCACGGG CTCTACGCGT
    GTTCCACTTC AGGGCTTTAA AGCTCTTCAA GGTGCTGCTG GACCCAGACT TTTTACTATT
    CATCTGATTG ATGCCAACAC AGATAACCTT CCAAAGGCTC ACACGTGCTT TAACCGAATT
    GACATTCCTC CTTATGAATC CTATGAAAAG CTCTATGAGA AATTATTAAC AGCTGTAGAA
    GAGACTAGTG GTTTTGCTGT GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa66392
    Clone ID Related Accession (Same CDS sequence) XM_031892958.1
    Accession Version XM_031892958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase SMURF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGACAGTTT TATGTGCGAA GAACCTAGCA AAGAGGGATT TTTTCAGATT ACCTGACCCA 
    TTTGCTAAAA TAGTTGTGGA TGGATCTGGT CAGTGCCACT CAACTGACAC TGTAAAGAAC
    ACATTAGACC CAAAGTGGAA TCAGCATTAT GACCTATATG TTGGTAAAAT GGATTCAATT
    ACAATCAGTA TCTGGAATCA TAAGAAAATT CACAAGAAAC AGGGAGCTGG CTTCCTGGGG
    TGTGTGAGAT TATTATCCAA TGCCATTAGC AGATTGAAAG ACACAGGCTA CCAGCGCTTG
    GATCTATGCA AATTAAATCC CGCAGATAGT GATGCTGTAC GGGGCCAAAT TGTAGTCAGT
    TTGCAGACAC GAGACAGGAT CGGTACAGTT GGCTCTGTTG TTGATTGCAG AGGACTGCTA
    GACAATGATG GAGCACTTTA TGAAGATGCT GGGCCTGGCA GGCCCCTTAG TTGTTTCATG
    GATGAGCCAG CCCCTTATAC TGATGGACCA GGTGCAGCTG GAGGAGGGCC CTGTAGGCTA
    GTAGAATCGC CAGGTCAAGA ACAGAGACTC CAAGCACAGA GACTTCGTGG ACCAGAAGTT
    AGAGAGCATG TCCAGACACC TTTAAATCGA TCCCATGGGT ACCAGTCACA GGACTTGCCT
    GAAGGTTATG AACAAAGAAC TACGGTTCAA GGGCAGGTGT ATTTCTTGCA CACACAAACT
    GGTGTCAGCA CATGGCATGA TCCCCGAATA CCAAGAGACC TTAACAGTGT GAATTGTGAT
    GACCTTGGCC CTTTGCCCAC TGGATGGGAA GTCAGAACCA CTGTGTCTGG AAGAATATAC
    TTTGTAGACC ACAATAATCG AACAACGCAG TTCACAGACC CAAGACTACA TCACATCATA
    AATCATCAGT CCCAGCTAAA AGAGCCAAAT AATGCTATAC CAGTGCAGAG TGATGGATCT
    TTAGAAGAGG GGGATGAATT TCCTACTCAA AGATATGAGA GAGACTTGGT ACAGAAACTT
    AAAGTCCTGA GACATGAGTT ATCATTGCTG CAGCCTCAGG CAGGGCACTG CCGTGTTGAA
    GTGTCAAGGG AAGAAATTTT TGAGGAGTCC TATAGGCAGA TAATGAAGAT GAGACCGAAA
    GACCTTAAGA AAAGATTGAT GGTGAAATTT CGTGGGGAAG AAGGACTTGA CTATGGTGGA
    GTGGCCAGGG AGTGGCTGTA TTTACTATGC CATGAAATGC TGAATCCATA TTATGGACTT
    TTCCAATATT CTACAGACAA TATTTATACT CTGCAAATTA ATCCTGATTC ATCTATTAAT
    CCAGATCATT TATCTTATTT CCATTTTGTT GGTCGAATCA TGGGATTGGC TGTGTTTCAT
    GGACACTACA TTAATGGGGG GTTTACAGTT CCTTTCTACA AACAACTTTT GGGAAAGCCC
    ATTCAGCTGT CTGACTTGGA ATCTGTGGAT CCAGAGCTGC ATAAAAGCTT GGTTTGGATT
    TTAGAGAATG ACATTACTTC AGTATTAGAC CACACGTTCT GTGTTGAGCA CAATGCATTT
    GGCCGACTCC TCCAGCATGA ATTGAAACCA AATGGCAAGA ACTTACAAGT AACAGAAGAA
    AATAAGAAAG AGTATGTGAG GTTGTATGTT AATTGGAGGT TCATGAGAGG GATAGAGGCT
    CAGTTCTTGG CTCTGCAGAA AGGTTTTAAT GAGCTAATAC CACAACATTT GTTGAAGCCA
    TTTGAGCAAA AAGAACTAGA GCTTATTATT GGAGGCTTGG ATAAAATTGA CATCAGTGAC
    TGGAAGGCAA ACACTAGACT AAAACACTGC TTAGCTAATA GCAATATTGT TCAGTGGTTC
    TGGCAAGCAG TGGAGTCGTT TGATGAGGAA AGAAGGGCCA GACTTCTGCA GTTTGTCACG
    GGCTCTACGC GTGTTCCACT TCAGGGCTTT AAAGCTCTTC AAGGTTCTAC AGGTGCTGCT
    GGACCCAGAC TTTTTACTAT TCATCTGATT GATGCCAACA CAGATAACCT TCCAAAGGCT
    CACACGTGCT TTAACCGAAT TGACATTCCT CCTTATGAAT CCTATGAAAA GCTCTATGAG
    AAATTATTAA CAGCTGTAGA AGAGACTAGT GGTTTTGCTG TGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748818.1
    CDS16..2163
    Translation

    Target ORF information:

    RefSeq Version XM_031892958.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis SMAD specific E3 ubiquitin protein ligase 1 (smurf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGACAGTTT TATGTGCGAA GAACCTAGCA AAGAGGGATT TTTTCAGATT ACCTGACCCA 
    TTTGCTAAAA TAGTTGTGGA TGGATCTGGT CAGTGCCACT CAACTGACAC TGTAAAGAAC
    ACATTAGACC CAAAGTGGAA TCAGCATTAT GACCTATATG TTGGTAAAAT GGATTCAATT
    ACAATCAGTA TCTGGAATCA TAAGAAAATT CACAAGAAAC AGGGAGCTGG CTTCCTGGGG
    TGTGTGAGAT TATTATCCAA TGCCATTAGC AGATTGAAAG ACACAGGCTA CCAGCGCTTG
    GATCTATGCA AATTAAATCC CGCAGATAGT GATGCTGTAC GGGGCCAAAT TGTAGTCAGT
    TTGCAGACAC GAGACAGGAT CGGTACAGTT GGCTCTGTTG TTGATTGCAG AGGACTGCTA
    GACAATGATG GAGCACTTTA TGAAGATGCT GGGCCTGGCA GGCCCCTTAG TTGTTTCATG
    GATGAGCCAG CCCCTTATAC TGATGGACCA GGTGCAGCTG GAGGAGGGCC CTGTAGGCTA
    GTAGAATCGC CAGGTCAAGA ACAGAGACTC CAAGCACAGA GACTTCGTGG ACCAGAAGTT
    AGAGAGCATG TCCAGACACC TTTAAATCGA TCCCATGGGT ACCAGTCACA GGACTTGCCT
    GAAGGTTATG AACAAAGAAC TACGGTTCAA GGGCAGGTGT ATTTCTTGCA CACACAAACT
    GGTGTCAGCA CATGGCATGA TCCCCGAATA CCAAGAGACC TTAACAGTGT GAATTGTGAT
    GACCTTGGCC CTTTGCCCAC TGGATGGGAA GTCAGAACCA CTGTGTCTGG AAGAATATAC
    TTTGTAGACC ACAATAATCG AACAACGCAG TTCACAGACC CAAGACTACA TCACATCATA
    AATCATCAGT CCCAGCTAAA AGAGCCAAAT AATGCTATAC CAGTGCAGAG TGATGGATCT
    TTAGAAGAGG GGGATGAATT TCCTACTCAA AGATATGAGA GAGACTTGGT ACAGAAACTT
    AAAGTCCTGA GACATGAGTT ATCATTGCTG CAGCCTCAGG CAGGGCACTG CCGTGTTGAA
    GTGTCAAGGG AAGAAATTTT TGAGGAGTCC TATAGGCAGA TAATGAAGAT GAGACCGAAA
    GACCTTAAGA AAAGATTGAT GGTGAAATTT CGTGGGGAAG AAGGACTTGA CTATGGTGGA
    GTGGCCAGGG AGTGGCTGTA TTTACTATGC CATGAAATGC TGAATCCATA TTATGGACTT
    TTCCAATATT CTACAGACAA TATTTATACT CTGCAAATTA ATCCTGATTC ATCTATTAAT
    CCAGATCATT TATCTTATTT CCATTTTGTT GGTCGAATCA TGGGATTGGC TGTGTTTCAT
    GGACACTACA TTAATGGGGG GTTTACAGTT CCTTTCTACA AACAACTTTT GGGAAAGCCC
    ATTCAGCTGT CTGACTTGGA ATCTGTGGAT CCAGAGCTGC ATAAAAGCTT GGTTTGGATT
    TTAGAGAATG ACATTACTTC AGTATTAGAC CACACGTTCT GTGTTGAGCA CAATGCATTT
    GGCCGACTCC TCCAGCATGA ATTGAAACCA AATGGCAAGA ACTTACAAGT AACAGAAGAA
    AATAAGAAAG AGTATGTGAG GTTGTATGTT AATTGGAGGT TCATGAGAGG GATAGAGGCT
    CAGTTCTTGG CTCTGCAGAA AGGTTTTAAT GAGCTAATAC CACAACATTT GTTGAAGCCA
    TTTGAGCAAA AAGAACTAGA GCTTATTATT GGAGGCTTGG ATAAAATTGA CATCAGTGAC
    TGGAAGGCAA ACACTAGACT AAAACACTGC TTAGCTAATA GCAATATTGT TCAGTGGTTC
    TGGCAAGCAG TGGAGTCGTT TGATGAGGAA AGAAGGGCCA GACTTCTGCA GTTTGTCACG
    GGCTCTACGC GTGTTCCACT TCAGGGCTTT AAAGCTCTTC AAGGTTCTAC AGGTGCTGCT
    GGACCCAGAC TTTTTACTAT TCATCTGATT GATGCCAACA CAGATAACCT TCCAAAGGCT
    CACACGTGCT TTAACCGAAT TGACATTCCT CCTTATGAAT CCTATGAAAA GCTCTATGAG
    AAATTATTAA CAGCTGTAGA AGAGACTAGT GGTTTTGCTG TGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.