dgki cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following dgki gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgki cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54180 XM_031898545.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa54181 XM_031898546.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa43477 XM_018092592.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa54173 XM_031898538.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54174 XM_031898539.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54175 XM_031898540.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54176 XM_031898541.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54177 XM_031898542.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54178 XM_031898543.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa54179 XM_031898544.1
Latest version!
Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54180
    Clone ID Related Accession (Same CDS sequence) XM_031898545.1
    Accession Version XM_031898545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCC CGCCCGACGG AGCCCAGAGC CGGGAGAACG GATGCCGGGG TGCGGAGGGG 
    GGGCACGAAA CGGCCGGGGA AGAGCCGGGA ACCGGGCACC GGGAAAGGGA GGAGTCGGTG
    GAGGAAAAGC TCCGGAGCCT GACCTTCCGC AAGCAGGTGT CATACAGGAA AGCGATTTCT
    CGATCGGGGC TCCAGCATCT GGCTCCTGCG CACTGCCCTG TCTCCAATGG GCCGGCCAAA
    GAACTGAGGA TGACCCTGGA CTGGACTGAG AATGCCATTA ATGGGGAGCA CCTCTGGCAG
    GAGACAAATG TATCTGGAGA CCTGTGTTAC CTGGGGGAGG AGAACTGCCA AGTCAAATTC
    GCTAAGTCGG CAATTCGCAG GAAATGTGCA GCTTGCAAAA TAGTGGTCCA TACGGCCTGC
    ATGGAGCAGC TCGACAAGAT TAATTTTAGA TGCAAACCCA CTTTCCGCGA TGGAACCTCT
    CGGACCCCGA GGGAGCAGAA TCTGGTCAGA CACCACTGGG TCCATCGGCG GAGGCAGGAG
    GGCAAATGCA AGCAGTGTGG GAAGGGGTTC CAGCAGAAGT TTTCATTCCA TAGCAAGGAG
    ATTGTGGCAA TTAGCTGTTC CTGGTGCAAA CAGGCGTTCC ACAACAAGGT CACTTGCTTC
    CTGCTCCATC ACATAGAGGA GCCCTGCTCA TTGGGGGCCC ATGCAGCAGT CATAGTCCCT
    CCAAGCTGGA TCATTAAGGT GAAGAAACCT CAGGTTAATA CAAAAAATTC CACAAGAAGG
    AAGAAGAGGA CTTCATTCAA AAGGAGAGCC AGCAAAAAAG GTTTGGAGGA TATTAAAGGC
    AGGGCCTTTA TAATAAAACC AATCTCTTCC CCACTCATGA AACCATTGCT GGTCTTCGTG
    AACCCAAAAA GTGGAGGGAA CCAGGGTACG AAAGTTCTCC AGATGTTTAT GTGGTACTTG
    AATCCTCGGC AGGTCTTTGA CCTCTCCCAG GAAGGACCGC GGGGAGCGCT GGAGATGTAC
    AGAAAAGTGC CAAATCTTCG TATCCTTGCC TGCGGCGGTG ACGGGACGGT GGGCTGGATC
    CTTTCTGTGT TAGATGAGCT TCAGCTGAAC CCTCAGCCTC CTGTAGCAGT TTTGCCCCTC
    GGCACCGGGA ATGACCTTGC TCGCACCCTT AACTGGGGAG GGGGTTACAC CGACGAACCC
    GTGTCGAAGA TCCTGTGCCA CGTAGAAGAC GGCACCAACG TCCAATTGGA TCGCTGGAAC
    CTGCACGTGG AGCGGAACCC CGACTTACTC CACGAGGAAC TGGATGATGG GACACATAAG
    CTTCCACTGA ACGTATTCAA TAACTACTTC AGCCTGGGGT TCGACGCGCA CGTCACTCTG
    GAATTCCACG AATCACGAGA AGCAAATCCC GAGAAATTCA ACAGTCGATT TCGTAATAAG
    ATGTTCTATG CAGGGGCTGC TTTCTCTGAT TTCTTGCAAA GAAGCTCCCG GGACTTATCA
    AAGCACGTGA AGGTTGTGTG CGACGGCACC GACCTCACCC CCAAAATACA AGAACTGAAA
    TTCCAGTGTA TTGTCTTCTT AAATATCCCC AGGTACTGTG CTGGCACAAT GCCCTGGGGT
    AATCCCAGTG ACCACAGAGA CTTTGAGCCC CAGCGGCACG ATGATGGTTA TATAGAGGTC
    ATCGGCTTTA CTATGGCTTC TCTGGCCGCC CTGCAGGTTG GAGGCCACGG AGAGAGGCTG
    CACCAGTGCA GGGAGGTGAC ATTGTTGACA TACAAAGCCA TCCCTATGCA GGTCGATGGA
    GAACCATGTC GGCTTGCACC TTCCCTCATC CGCATATCCC TGAGGAACCA GGCCAACATG
    GTGCAGAAGA GCAAGCGGAG GACTTCCATG CCCTTACTTA ATGATCTCCA TAAAGTGCAC
    TCGGCCGACC ACCGACGCGT CTCCGCTCCC CCGGACTCCT TCTCCGTCCC CCATTCAGTG
    CCGGATCGCC TGCGTATCAG AGTGAACAGG ATCCGCCTGC AGGAATACGA GGGGCTCCAT
    TATGAAAAGG ACAAACTCCG AGACGCCTCT ATTCCCCTGG GGATCATTGT GGTACGAGGA
    GACTGTGACC TAGAGACCTG CAGGATGTAT ATCGACCGCC TCCAAGAGGA TTTACATTCT
    GTGACACCCC ACCGAGATCA CTGCCAGGAT CACGATAGCT CCTTCCCGCG GTTGCTCTCT
    GCTCAGCGAC TCAACCCTCG TTGGTGCTTC TTAGATGCTA CCTCCGCCGA CCGGTTTTAC
    CGCATTGACA GATCTCAGGA ACATCTGCAT TTTGTCACTG AGATTTCCCA AGATGAGATC
    TTCATTCTTG ACCCTGAAGC CGATGGGACC CCCCAAGGCA GCACTGGGAT GGGAATGCCA
    GATCTTGTGG TGGAGCAGCC GTCTGGGCCA TCGGAATGGT GGACCCCGAC GTCCCGTAAG
    CGGATAGTGA GTGACAGCTG TGTGGCGGGA AGGATGTCTC TCCAATATGA AATCTCAGAC
    AAAGTACACG ATTTGTCCCC ATGTAGCGCC CGCCTGTCAC CGGTCTCTGT GGATGAACAA
    GCACTCACAG AAGCAGTTGC CGCGGGTGAT CTTGCCAAGG TCACGGAGTG TTGCAAGAAG
    GGAATGAGTC TGCGCATCCC GGGGCCCGAG AGGGGCTCTC TGCTCCACCT GGCGGCACGG
    GCCGGGCACA GCGACATAGT GGCGTACATC CTACAGAATG GGCCCACAGA TTTGCTGGAC
    ATGTCAGACA GAGCCACCGG AGAGACGGCG CTGCACAAAG CTGCTTCCCA CGGCCACCGG
    CGGGTTTGCC AAATCCTAGT AGATGCCGGT GCCTGCGTGA TAAAGAAAGA CGCCAAGGGC
    AACACTCCTT TCGACAAAGC TCAGGAGGCA GGGGACTCGG AACTGGCATC GTACTTGGAC
    ACTTGTCAAG CCAAGCAACT TCTTTCCAAC GAAGACCTCG AGACCGCGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754405.1
    CDS113..3106
    Translation

    Target ORF information:

    RefSeq Version XM_031898545.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCC CGCCCGACGG AGCCCAGAGC CGGGAGAACG GATGCCGGGG TGCGGAGGGG 
    GGGCACGAAA CGGCCGGGGA AGAGCCGGGA ACCGGGCACC GGGAAAGGGA GGAGTCGGTG
    GAGGAAAAGC TCCGGAGCCT GACCTTCCGC AAGCAGGTGT CATACAGGAA AGCGATTTCT
    CGATCGGGGC TCCAGCATCT GGCTCCTGCG CACTGCCCTG TCTCCAATGG GCCGGCCAAA
    GAACTGAGGA TGACCCTGGA CTGGACTGAG AATGCCATTA ATGGGGAGCA CCTCTGGCAG
    GAGACAAATG TATCTGGAGA CCTGTGTTAC CTGGGGGAGG AGAACTGCCA AGTCAAATTC
    GCTAAGTCGG CAATTCGCAG GAAATGTGCA GCTTGCAAAA TAGTGGTCCA TACGGCCTGC
    ATGGAGCAGC TCGACAAGAT TAATTTTAGA TGCAAACCCA CTTTCCGCGA TGGAACCTCT
    CGGACCCCGA GGGAGCAGAA TCTGGTCAGA CACCACTGGG TCCATCGGCG GAGGCAGGAG
    GGCAAATGCA AGCAGTGTGG GAAGGGGTTC CAGCAGAAGT TTTCATTCCA TAGCAAGGAG
    ATTGTGGCAA TTAGCTGTTC CTGGTGCAAA CAGGCGTTCC ACAACAAGGT CACTTGCTTC
    CTGCTCCATC ACATAGAGGA GCCCTGCTCA TTGGGGGCCC ATGCAGCAGT CATAGTCCCT
    CCAAGCTGGA TCATTAAGGT GAAGAAACCT CAGGTTAATA CAAAAAATTC CACAAGAAGG
    AAGAAGAGGA CTTCATTCAA AAGGAGAGCC AGCAAAAAAG GTTTGGAGGA TATTAAAGGC
    AGGGCCTTTA TAATAAAACC AATCTCTTCC CCACTCATGA AACCATTGCT GGTCTTCGTG
    AACCCAAAAA GTGGAGGGAA CCAGGGTACG AAAGTTCTCC AGATGTTTAT GTGGTACTTG
    AATCCTCGGC AGGTCTTTGA CCTCTCCCAG GAAGGACCGC GGGGAGCGCT GGAGATGTAC
    AGAAAAGTGC CAAATCTTCG TATCCTTGCC TGCGGCGGTG ACGGGACGGT GGGCTGGATC
    CTTTCTGTGT TAGATGAGCT TCAGCTGAAC CCTCAGCCTC CTGTAGCAGT TTTGCCCCTC
    GGCACCGGGA ATGACCTTGC TCGCACCCTT AACTGGGGAG GGGGTTACAC CGACGAACCC
    GTGTCGAAGA TCCTGTGCCA CGTAGAAGAC GGCACCAACG TCCAATTGGA TCGCTGGAAC
    CTGCACGTGG AGCGGAACCC CGACTTACTC CACGAGGAAC TGGATGATGG GACACATAAG
    CTTCCACTGA ACGTATTCAA TAACTACTTC AGCCTGGGGT TCGACGCGCA CGTCACTCTG
    GAATTCCACG AATCACGAGA AGCAAATCCC GAGAAATTCA ACAGTCGATT TCGTAATAAG
    ATGTTCTATG CAGGGGCTGC TTTCTCTGAT TTCTTGCAAA GAAGCTCCCG GGACTTATCA
    AAGCACGTGA AGGTTGTGTG CGACGGCACC GACCTCACCC CCAAAATACA AGAACTGAAA
    TTCCAGTGTA TTGTCTTCTT AAATATCCCC AGGTACTGTG CTGGCACAAT GCCCTGGGGT
    AATCCCAGTG ACCACAGAGA CTTTGAGCCC CAGCGGCACG ATGATGGTTA TATAGAGGTC
    ATCGGCTTTA CTATGGCTTC TCTGGCCGCC CTGCAGGTTG GAGGCCACGG AGAGAGGCTG
    CACCAGTGCA GGGAGGTGAC ATTGTTGACA TACAAAGCCA TCCCTATGCA GGTCGATGGA
    GAACCATGTC GGCTTGCACC TTCCCTCATC CGCATATCCC TGAGGAACCA GGCCAACATG
    GTGCAGAAGA GCAAGCGGAG GACTTCCATG CCCTTACTTA ATGATCTCCA TAAAGTGCAC
    TCGGCCGACC ACCGACGCGT CTCCGCTCCC CCGGACTCCT TCTCCGTCCC CCATTCAGTG
    CCGGATCGCC TGCGTATCAG AGTGAACAGG ATCCGCCTGC AGGAATACGA GGGGCTCCAT
    TATGAAAAGG ACAAACTCCG AGACGCCTCT ATTCCCCTGG GGATCATTGT GGTACGAGGA
    GACTGTGACC TAGAGACCTG CAGGATGTAT ATCGACCGCC TCCAAGAGGA TTTACATTCT
    GTGACACCCC ACCGAGATCA CTGCCAGGAT CACGATAGCT CCTTCCCGCG GTTGCTCTCT
    GCTCAGCGAC TCAACCCTCG TTGGTGCTTC TTAGATGCTA CCTCCGCCGA CCGGTTTTAC
    CGCATTGACA GATCTCAGGA ACATCTGCAT TTTGTCACTG AGATTTCCCA AGATGAGATC
    TTCATTCTTG ACCCTGAAGC CGATGGGACC CCCCAAGGCA GCACTGGGAT GGGAATGCCA
    GATCTTGTGG TGGAGCAGCC GTCTGGGCCA TCGGAATGGT GGACCCCGAC GTCCCGTAAG
    CGGATAGTGA GTGACAGCTG TGTGGCGGGA AGGATGTCTC TCCAATATGA AATCTCAGAC
    AAAGTACACG ATTTGTCCCC ATGTAGCGCC CGCCTGTCAC CGGTCTCTGT GGATGAACAA
    GCACTCACAG AAGCAGTTGC CGCGGGTGAT CTTGCCAAGG TCACGGAGTG TTGCAAGAAG
    GGAATGAGTC TGCGCATCCC GGGGCCCGAG AGGGGCTCTC TGCTCCACCT GGCGGCACGG
    GCCGGGCACA GCGACATAGT GGCGTACATC CTACAGAATG GGCCCACAGA TTTGCTGGAC
    ATGTCAGACA GAGCCACCGG AGAGACGGCG CTGCACAAAG CTGCTTCCCA CGGCCACCGG
    CGGGTTTGCC AAATCCTAGT AGATGCCGGT GCCTGCGTGA TAAAGAAAGA CGCCAAGGGC
    AACACTCCTT TCGACAAAGC TCAGGAGGCA GGGGACTCGG AACTGGCATC GTACTTGGAC
    ACTTGTCAAG CCAAGCAACT TCTTTCCAAC GAAGACCTCG AGACCGCGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54181
    Clone ID Related Accession (Same CDS sequence) XM_031898546.1
    Accession Version XM_031898546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCC CGCCCGACGG AGCCCAGAGC CGGGAGAACG GATGCCGGGG TGCGGAGGGG 
    GGGCACGAAA CGGCCGGGGA AGAGCCGGGA ACCGGGCACC GGGAAAGGGA GGAGTCGGTG
    GAGGAAAAGC TCCGGAGCCT GACCTTCCGC AAGCAGGTGT CATACAGGAA AGCGATTTCT
    CGATCGGGGC TCCAGCATCT GGCTCCTGCG CACTGCCCTG TCTCCAATGG GCCGGCCAAA
    GAACTGAGGA TGACCCTGGA CTGGACTGAG AATGCCATTA ATGGGGAGCA CCTCTGGCAG
    GAGACAAATG TATCTGGAGA CCTGTGTTAC CTGGGGGAGG AGAACTGCCA AGTCAAATTC
    GCTAAGTCGG CAATTCGCAG GAAATGTGCA GCTTGCAAAA TAGTGGTCCA TACGGCCTGC
    ATGGAGCAGC TCGACAAGAT TAATTTTAGA TGCAAACCCA CTTTCCGCGA TGGAACCTCT
    CGGACCCCGA GGGAGCAGAA TCTGGTCAGA CACCACTGGG TCCATCGGCG GAGGCAGGAG
    GGCAAATGCA AGCAGTGTGG GAAGGGGTTC CAGCAGAAGT TTTCATTCCA TAGCAAGGAG
    ATTGTGGCAA TTAGCTGTTC CTGGTGCAAA CAGGCGTTCC ACAACAAGGT CACTTGCTTC
    CTGCTCCATC ACATAGAGGA GCCCTGCTCA TTGGGGGCCC ATGCAGCAGT CATAGTCCCT
    CCAAGCTGGA TCATTAAGGT TAATACAAAA AATTCCACAA GAAGGAAGAA GAGGACTTCA
    TTCAAAAGGA GAGCCAGCAA AAAAGGTTTG GAGGATATTA AAGGCAGGGC CTTTATAATA
    AAACCAATCT CTTCCCCACT CATGAAACCA TTGCTGGTCT TCGTGAACCC AAAAAGTGGA
    GGGAACCAGG GTACGAAAGT TCTCCAGATG TTTATGTGGT ACTTGAATCC TCGGCAGGTC
    TTTGACCTCT CCCAGGAAGG ACCGCGGGGA GCGCTGGAGA TGTACAGAAA AGTGCCAAAT
    CTTCGTATCC TTGCCTGCGG CGGTGACGGG ACGGTGGGCT GGATCCTTTC TGTGTTAGAT
    GAGCTTCAGC TGAACCCTCA GCCTCCTGTA GCAGTTTTGC CCCTCGGCAC CGGGAATGAC
    CTTGCTCGCA CCCTTAACTG GGGAGGGGGT TACACCGACG AACCCGTGTC GAAGATCCTG
    TGCCACGTAG AAGACGGCAC CAACGTCCAA TTGGATCGCT GGAACCTGCA CGTGGAGCGG
    AACCCCGACT TACTCCACGA GGAACTGGAT GATGGGACAC ATAAGCTTCC ACTGAACGTA
    TTCAATAACT ACTTCAGCCT GGGGTTCGAC GCGCACGTCA CTCTGGAATT CCACGAATCA
    CGAGAAGCAA ATCCCGAGAA ATTCAACAGT CGATTTCGTA ATAAGATGTT CTATGCAGGG
    GCTGCTTTCT CTGATTTCTT GCAAAGAAGC TCCCGGGACT TATCAAAGCA CGTGAAGGTT
    GTGTGCGACG GCACCGACCT CACCCCCAAA ATACAAGAAC TGAAATTCCA GTGTATTGTC
    TTCTTAAATA TCCCCAGGTA CTGTGCTGGC ACAATGCCCT GGGGTAATCC CAGTGACCAC
    AGAGACTTTG AGCCCCAGCG GCACGATGAT GGTTATATAG AGGTCATCGG CTTTACTATG
    GCTTCTCTGG CCGCCCTGCA GGTTGGAGGC CACGGAGAGA GGCTGCACCA GTGCAGGGAG
    GTGACATTGT TGACATACAA AGCCATCCCT ATGCAGGTCG ATGGAGAACC ATGTCGGCTT
    GCACCTTCCC TCATCCGCAT ATCCCTGAGG AACCAGGCCA ACATGGTGCA GAAGAGCAAG
    CGGAGGACTT CCATGCCCTT ACTTAATGAT CTCCATAAAG TGCACTCGGC CGACCACCGA
    CGCGTCTCCG CTCCCCCGGA CTCCTTCTCC GTCCCCCATT CAGTGCCGGA TCGCCTGCGT
    ATCAGAGTGA ACAGGATCCG CCTGCAGGAA TACGAGGGGC TCCATTATGA AAAGGACAAA
    CTCCGAGACG CCTCTATTCC CCTGGGGATC ATTGTGGTAC GAGGAGACTG TGACCTAGAG
    ACCTGCAGGA TGTATATCGA CCGCCTCCAA GAGGATTTAC ATTCTGTGAC ACCCCACCGA
    GATCACTGCC AGGATCACGA TAGCTCCTTC CCGCGGTTGC TCTCTGCTCA GCGACTCAAC
    CCTCGTTGGT GCTTCTTAGA TGCTACCTCC GCCGACCGGT TTTACCGCAT TGACAGATCT
    CAGGAACATC TGCATTTTGT CACTGAGATT TCCCAAGATG AGATCTTCAT TCTTGACCCT
    GAAGCCGATG GGACCCCCCA AGGCAGCACT GGGATGGGAA TGCCAGATCT TGTGGTGGAG
    CAGCCGTCTG GGCCATCGGA ATGGTGGACC CCGACGTCCC GTAAGCGGAT AGTGAGTGAC
    AGCTGTGTGG CGGGAAGGAT GTCTCTCCAA TATGAAATCT CAGACAAAGT ACACGATTTG
    TCCCCATGTA GCGCCCGCCT GTCACCGGTC TCTGTGGATG AACAAGCACT CACAGAAGCA
    GTTGCCGCGG GTGATCTTGC CAAGGTCACG GAGTGTTGCA AGAAGGGAAT GAGTCTGCGC
    ATCCCGGGGC CCGAGAGGGG CTCTCTGCTC CACCTGGCGG CACGGGCCGG GCACAGCGAC
    ATAGTGGCGT ACATCCTACA GAATGGGCCC ACAGATTTGC TGGACATGTC AGACAGAGCC
    ACCGGAGAGA CGGCGCTGCA CAAAGCTGCT TCCCACGGCC ACCGGCGGGT TTGCCAAATC
    CTAGTAGATG CCGGTGCCTG CGTGATAAAG AAAGACGCCA AGGGCAACAC TCCTTTCGAC
    AAAGCTCAGG AGGCAGGGGA CTCGGAACTG GCATCGTACT TGGACACTTG TCAAGCCAAG
    CAACTTCTTT CCAACGAAGA CCTCGAGACC GCGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754406.1
    CDS113..3091
    Translation

    Target ORF information:

    RefSeq Version XM_031898546.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCC CGCCCGACGG AGCCCAGAGC CGGGAGAACG GATGCCGGGG TGCGGAGGGG 
    GGGCACGAAA CGGCCGGGGA AGAGCCGGGA ACCGGGCACC GGGAAAGGGA GGAGTCGGTG
    GAGGAAAAGC TCCGGAGCCT GACCTTCCGC AAGCAGGTGT CATACAGGAA AGCGATTTCT
    CGATCGGGGC TCCAGCATCT GGCTCCTGCG CACTGCCCTG TCTCCAATGG GCCGGCCAAA
    GAACTGAGGA TGACCCTGGA CTGGACTGAG AATGCCATTA ATGGGGAGCA CCTCTGGCAG
    GAGACAAATG TATCTGGAGA CCTGTGTTAC CTGGGGGAGG AGAACTGCCA AGTCAAATTC
    GCTAAGTCGG CAATTCGCAG GAAATGTGCA GCTTGCAAAA TAGTGGTCCA TACGGCCTGC
    ATGGAGCAGC TCGACAAGAT TAATTTTAGA TGCAAACCCA CTTTCCGCGA TGGAACCTCT
    CGGACCCCGA GGGAGCAGAA TCTGGTCAGA CACCACTGGG TCCATCGGCG GAGGCAGGAG
    GGCAAATGCA AGCAGTGTGG GAAGGGGTTC CAGCAGAAGT TTTCATTCCA TAGCAAGGAG
    ATTGTGGCAA TTAGCTGTTC CTGGTGCAAA CAGGCGTTCC ACAACAAGGT CACTTGCTTC
    CTGCTCCATC ACATAGAGGA GCCCTGCTCA TTGGGGGCCC ATGCAGCAGT CATAGTCCCT
    CCAAGCTGGA TCATTAAGGT TAATACAAAA AATTCCACAA GAAGGAAGAA GAGGACTTCA
    TTCAAAAGGA GAGCCAGCAA AAAAGGTTTG GAGGATATTA AAGGCAGGGC CTTTATAATA
    AAACCAATCT CTTCCCCACT CATGAAACCA TTGCTGGTCT TCGTGAACCC AAAAAGTGGA
    GGGAACCAGG GTACGAAAGT TCTCCAGATG TTTATGTGGT ACTTGAATCC TCGGCAGGTC
    TTTGACCTCT CCCAGGAAGG ACCGCGGGGA GCGCTGGAGA TGTACAGAAA AGTGCCAAAT
    CTTCGTATCC TTGCCTGCGG CGGTGACGGG ACGGTGGGCT GGATCCTTTC TGTGTTAGAT
    GAGCTTCAGC TGAACCCTCA GCCTCCTGTA GCAGTTTTGC CCCTCGGCAC CGGGAATGAC
    CTTGCTCGCA CCCTTAACTG GGGAGGGGGT TACACCGACG AACCCGTGTC GAAGATCCTG
    TGCCACGTAG AAGACGGCAC CAACGTCCAA TTGGATCGCT GGAACCTGCA CGTGGAGCGG
    AACCCCGACT TACTCCACGA GGAACTGGAT GATGGGACAC ATAAGCTTCC ACTGAACGTA
    TTCAATAACT ACTTCAGCCT GGGGTTCGAC GCGCACGTCA CTCTGGAATT CCACGAATCA
    CGAGAAGCAA ATCCCGAGAA ATTCAACAGT CGATTTCGTA ATAAGATGTT CTATGCAGGG
    GCTGCTTTCT CTGATTTCTT GCAAAGAAGC TCCCGGGACT TATCAAAGCA CGTGAAGGTT
    GTGTGCGACG GCACCGACCT CACCCCCAAA ATACAAGAAC TGAAATTCCA GTGTATTGTC
    TTCTTAAATA TCCCCAGGTA CTGTGCTGGC ACAATGCCCT GGGGTAATCC CAGTGACCAC
    AGAGACTTTG AGCCCCAGCG GCACGATGAT GGTTATATAG AGGTCATCGG CTTTACTATG
    GCTTCTCTGG CCGCCCTGCA GGTTGGAGGC CACGGAGAGA GGCTGCACCA GTGCAGGGAG
    GTGACATTGT TGACATACAA AGCCATCCCT ATGCAGGTCG ATGGAGAACC ATGTCGGCTT
    GCACCTTCCC TCATCCGCAT ATCCCTGAGG AACCAGGCCA ACATGGTGCA GAAGAGCAAG
    CGGAGGACTT CCATGCCCTT ACTTAATGAT CTCCATAAAG TGCACTCGGC CGACCACCGA
    CGCGTCTCCG CTCCCCCGGA CTCCTTCTCC GTCCCCCATT CAGTGCCGGA TCGCCTGCGT
    ATCAGAGTGA ACAGGATCCG CCTGCAGGAA TACGAGGGGC TCCATTATGA AAAGGACAAA
    CTCCGAGACG CCTCTATTCC CCTGGGGATC ATTGTGGTAC GAGGAGACTG TGACCTAGAG
    ACCTGCAGGA TGTATATCGA CCGCCTCCAA GAGGATTTAC ATTCTGTGAC ACCCCACCGA
    GATCACTGCC AGGATCACGA TAGCTCCTTC CCGCGGTTGC TCTCTGCTCA GCGACTCAAC
    CCTCGTTGGT GCTTCTTAGA TGCTACCTCC GCCGACCGGT TTTACCGCAT TGACAGATCT
    CAGGAACATC TGCATTTTGT CACTGAGATT TCCCAAGATG AGATCTTCAT TCTTGACCCT
    GAAGCCGATG GGACCCCCCA AGGCAGCACT GGGATGGGAA TGCCAGATCT TGTGGTGGAG
    CAGCCGTCTG GGCCATCGGA ATGGTGGACC CCGACGTCCC GTAAGCGGAT AGTGAGTGAC
    AGCTGTGTGG CGGGAAGGAT GTCTCTCCAA TATGAAATCT CAGACAAAGT ACACGATTTG
    TCCCCATGTA GCGCCCGCCT GTCACCGGTC TCTGTGGATG AACAAGCACT CACAGAAGCA
    GTTGCCGCGG GTGATCTTGC CAAGGTCACG GAGTGTTGCA AGAAGGGAAT GAGTCTGCGC
    ATCCCGGGGC CCGAGAGGGG CTCTCTGCTC CACCTGGCGG CACGGGCCGG GCACAGCGAC
    ATAGTGGCGT ACATCCTACA GAATGGGCCC ACAGATTTGC TGGACATGTC AGACAGAGCC
    ACCGGAGAGA CGGCGCTGCA CAAAGCTGCT TCCCACGGCC ACCGGCGGGT TTGCCAAATC
    CTAGTAGATG CCGGTGCCTG CGTGATAAAG AAAGACGCCA AGGGCAACAC TCCTTTCGAC
    AAAGCTCAGG AGGCAGGGGA CTCGGAACTG GCATCGTACT TGGACACTTG TCAAGCCAAG
    CAACTTCTTT CCAACGAAGA CCTCGAGACC GCGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43477
    Clone ID Related Accession (Same CDS sequence) XM_018092592.1
    Accession Version XM_018092592.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTCTACT CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGGTAAGAGA TCGCCATCTA
    CTGGCCGCGG AGGGAATGGT TATCTTTGCT GGAACCAGTG GAAGCCAATG GCTGTGCAGT
    TTATCCATCA TCGTTAGTCA TGTGACTGTC AGACATTTTT CTTGTTGTTC TAACCAGCAG
    AATCTGGTCA GACACCACTG GGTCCATCGG CGGAGGCAGG AGGGCAAATG CAAGCAGTGT
    GGGAAGGGGT TCCAGCAGAA GTTTTCATTC CATAGCAAGG AGATTGTGGC AATTAGCTGT
    TCCTGGTGCA AACAGGCGTT CCACAACAAG GTCACTTGCT TCCTGCTCCA TCACATAGAG
    GAGCCCTGCT CATTGGGGGC CCATGCAGCA GTCATAGTCC CTCCAAGCTG GATCATTAAG
    GTGAAGAAAC CTCAGGTTAA TACAAAAAAT TCCACAAGAA GGAAGAAGAG GACTTCATTC
    AAAAGGAGAG CCAGCAAAAA AGGTTTGGAG GATATTAAAG GCAGGGCCTT TATAATAAAA
    CCAATCTCTT CCCCACTCAT GAAACCATTG CTGGTCTTCG TGAACCCAAA AAGTGGAGGG
    AACCAGGGTA CGAAAGTTCT CCAGATGTTT ATGTGGTACT TGAATCCTCG GCAGGTCTTT
    GACCTCTCCC AGGAAGGACC GCGGGGAGCG CTGGAGATGT ACAGAAAAGT GCCAAATCTT
    CGTATCCTTG CCTGCGGCGG TGACGGGACG GTGGGCTGGA TCCTTTCTGT GTTAGATGAG
    CTTCAGCTGA ACCCTCAGCC TCCTGTAGCA GTTTTGCCCC TCGGCACCGG GAATGACCTT
    GCTCGCACCC TTAACTGGGG AGGGGGCTAC ACCGACGAAC CCGTGTCGAA GATCCTGTGC
    CACGTAGAAG ACGGCACCAA CGTCCAATTG GATCGCTGGA ACCTGCACGT GGAGCGGAAC
    CCCGACTTAC TCCACGAGGA ACTGGATGAT GGGACACATA AGCTTCCACT GAACGTATTC
    AATAACTACT TCAGCCTGGG GTTCGACGCG CACGTCACTC TCGAATTCCA CGAATCACGA
    GGTACGTTGG GGAACCCCTT GAGAAACCGC CGTGGATATG CTTATTTGTT AAATTTACCC
    TCCCTCACTC CCTTTCAGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGACC CCCATTCAGT GCCGGATCGC
    CTGCGTATCA GAGTGAACAG GATCCGCCTG CAGGAATACG AGGGGCTCCA TTATGAAAAG
    GACAAACTCC GAGACGCCTC TATTCCCCTG GGGATCATTG TGGTACGAGG AGACTGTGAC
    CTAGAGACCT GCAGGATGTA TATCGACCGC CTCCAAGAGG ATTTACATTC TGTGACACCC
    CACCGAGATC ACTGCCAGGA TCACGATAGC TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA
    CTCAACCCTC GTTGGTGCTT CTTAGATGCT ACCTCCGCCG ACCGGTTTTA CCGCATTGAC
    AGATCTCAGG AACATCTGCA TTTTGTCACT GAGATTTCCC AAGATGAGAT CTTCATTCTT
    GACCCTGAAG CCGATGGGAC CCCCCAAGGC AGCACTGGGA TGGGAATGCC AGATCTTGTG
    GTGGAGCAGC CGTCTGGGCC ATCGGAATGG TGGACCCCGA CGTCCCGTAA GCGGATAGTG
    AGTGACAGCT GTGTGGCGGG AAGGATGTCT CTCCAATATG AAATCTCAGA CAAAGTACAC
    GATTTGTCCC CATGTAGCGC CCGCCTGTCA CCGGTCTCTG TGGATGAACA AGCACTCACA
    GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG GTCACGGAGT GTTGCAAGAA GGGAATGAGT
    CTGCGCATCC CGGGGCCCGA GAGGGGCTCT CTGCTCCACC TGGCGGCACG GGCCGGGCAC
    AGCGACATAG TGGCGTACAT CCTACAGAAT GGTGAGTACA GGCTCAATGG TTTTGTCTTC
    TTCTTTGCGC CCCCCTCTCC CCCTTCCCTC GCTCCTCTCG GCCCCCGCTG GGCACTGACT
    GACAGTCGTT TTTCTTTTTT CTCTTGGTTT AGCGGAGAGA CGGCGCTGCA CAAAGCTGCT
    TCCCACGGCC ACCGGCGGGT TTGCCAAATC CTAGTAGATG CCGGTGCCTG CGTGATAAAG
    AAAGACGCCA AGGGCAACAC TCCTTTCGAC AAAGCTCAGG AGGCAGGGGA CTCGGAACTG
    GCATCGTACT TGGACACTTG TCAAGCCAAG CAACTTCTTT CCAACGAAGA CCTCGAGACC
    GCGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017948081.1
    CDS2642..5770
    Translation

    Target ORF information:

    RefSeq Version XM_018092592.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092592.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTCTACT CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGGTAAGAGA TCGCCATCTA
    CTGGCCGCGG AGGGAATGGT TATCTTTGCT GGAACCAGTG GAAGCCAATG GCTGTGCAGT
    TTATCCATCA TCGTTAGTCA TGTGACTGTC AGACATTTTT CTTGTTGTTC TAACCAGCAG
    AATCTGGTCA GACACCACTG GGTCCATCGG CGGAGGCAGG AGGGCAAATG CAAGCAGTGT
    GGGAAGGGGT TCCAGCAGAA GTTTTCATTC CATAGCAAGG AGATTGTGGC AATTAGCTGT
    TCCTGGTGCA AACAGGCGTT CCACAACAAG GTCACTTGCT TCCTGCTCCA TCACATAGAG
    GAGCCCTGCT CATTGGGGGC CCATGCAGCA GTCATAGTCC CTCCAAGCTG GATCATTAAG
    GTGAAGAAAC CTCAGGTTAA TACAAAAAAT TCCACAAGAA GGAAGAAGAG GACTTCATTC
    AAAAGGAGAG CCAGCAAAAA AGGTTTGGAG GATATTAAAG GCAGGGCCTT TATAATAAAA
    CCAATCTCTT CCCCACTCAT GAAACCATTG CTGGTCTTCG TGAACCCAAA AAGTGGAGGG
    AACCAGGGTA CGAAAGTTCT CCAGATGTTT ATGTGGTACT TGAATCCTCG GCAGGTCTTT
    GACCTCTCCC AGGAAGGACC GCGGGGAGCG CTGGAGATGT ACAGAAAAGT GCCAAATCTT
    CGTATCCTTG CCTGCGGCGG TGACGGGACG GTGGGCTGGA TCCTTTCTGT GTTAGATGAG
    CTTCAGCTGA ACCCTCAGCC TCCTGTAGCA GTTTTGCCCC TCGGCACCGG GAATGACCTT
    GCTCGCACCC TTAACTGGGG AGGGGGCTAC ACCGACGAAC CCGTGTCGAA GATCCTGTGC
    CACGTAGAAG ACGGCACCAA CGTCCAATTG GATCGCTGGA ACCTGCACGT GGAGCGGAAC
    CCCGACTTAC TCCACGAGGA ACTGGATGAT GGGACACATA AGCTTCCACT GAACGTATTC
    AATAACTACT TCAGCCTGGG GTTCGACGCG CACGTCACTC TCGAATTCCA CGAATCACGA
    GGTACGTTGG GGAACCCCTT GAGAAACCGC CGTGGATATG CTTATTTGTT AAATTTACCC
    TCCCTCACTC CCTTTCAGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGACC CCCATTCAGT GCCGGATCGC
    CTGCGTATCA GAGTGAACAG GATCCGCCTG CAGGAATACG AGGGGCTCCA TTATGAAAAG
    GACAAACTCC GAGACGCCTC TATTCCCCTG GGGATCATTG TGGTACGAGG AGACTGTGAC
    CTAGAGACCT GCAGGATGTA TATCGACCGC CTCCAAGAGG ATTTACATTC TGTGACACCC
    CACCGAGATC ACTGCCAGGA TCACGATAGC TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA
    CTCAACCCTC GTTGGTGCTT CTTAGATGCT ACCTCCGCCG ACCGGTTTTA CCGCATTGAC
    AGATCTCAGG AACATCTGCA TTTTGTCACT GAGATTTCCC AAGATGAGAT CTTCATTCTT
    GACCCTGAAG CCGATGGGAC CCCCCAAGGC AGCACTGGGA TGGGAATGCC AGATCTTGTG
    GTGGAGCAGC CGTCTGGGCC ATCGGAATGG TGGACCCCGA CGTCCCGTAA GCGGATAGTG
    AGTGACAGCT GTGTGGCGGG AAGGATGTCT CTCCAATATG AAATCTCAGA CAAAGTACAC
    GATTTGTCCC CATGTAGCGC CCGCCTGTCA CCGGTCTCTG TGGATGAACA AGCACTCACA
    GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG GTCACGGAGT GTTGCAAGAA GGGAATGAGT
    CTGCGCATCC CGGGGCCCGA GAGGGGCTCT CTGCTCCACC TGGCGGCACG GGCCGGGCAC
    AGCGACATAG TGGCGTACAT CCTACAGAAT GGTGAGTACA GGCTCAATGG TTTTGTCTTC
    TTCTTTGCGC CCCCCTCTCC CCCTTCCCTC GCTCCTCTCG GCCCCCGCTG GGCACTGACT
    GACAGTCGTT TTTCTTTTTT CTCTTGGTTT AGCGGAGAGA CGGCGCTGCA CAAAGCTGCT
    TCCCACGGCC ACCGGCGGGT TTGCCAAATC CTAGTAGATG CCGGTGCCTG CGTGATAAAG
    AAAGACGCCA AGGGCAACAC TCCTTTCGAC AAAGCTCAGG AGGCAGGGGA CTCGGAACTG
    GCATCGTACT TGGACACTTG TCAAGCCAAG CAACTTCTTT CCAACGAAGA CCTCGAGACC
    GCGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54173
    Clone ID Related Accession (Same CDS sequence) XM_031898538.1
    Accession Version XM_031898538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTT AATACAAAAA ATTCCACAAG AAGGAAGAAG
    AGGACTTCAT TCAAAAGGAG AGCCAGCAAA AAAGGTTTGG AGGATATTAA AGGCAGGGCC
    TTTATAATAA AACCAATCTC TTCCCCACTC ATGAAACCAT TGCTGGTCTT CGTGAACCCA
    AAAAGTGGAG GGAACCAGGG TACGAAAGTT CTCCAGATGT TTATGTGGTA CTTGAATCCT
    CGGCAGGTCT TTGACCTCTC CCAGGAAGGA CCGCGGGGAG CGCTGGAGAT GTACAGAAAA
    GTGCCAAATC TTCGTATCCT TGCCTGCGGC GGTGACGGGA CGGTGGGCTG GATCCTTTCT
    GTGTTAGATG AGCTTCAGCT GAACCCTCAG CCTCCTGTAG CAGTTTTGCC CCTCGGCACC
    GGGAATGACC TTGCTCGCAC CCTTAACTGG GGAGGGGGTT ACACCGACGA ACCCGTGTCG
    AAGATCCTGT GCCACGTAGA AGACGGCACC AACGTCCAAT TGGATCGCTG GAACCTGCAC
    GTGGAGCGGA ACCCCGACTT ACTCCACGAG GAACTGGATG ATGGGACACA TAAGCTTCCA
    CTGAACGTAT TCAATAACTA CTTCAGCCTG GGGTTCGACG CGCACGTCAC TCTGGAATTC
    CACGAATCAC GAGAAGCAAA TCCCGAGAAA TTCAACAGTC GATTTCGTAA TAAGATGTTC
    TATGCAGGGG CTGCTTTCTC TGATTTCTTG CAAAGAAGCT CCCGGGACTT ATCAAAGCAC
    GTGAAGGTTG TGTGCGACGG CACCGACCTC ACCCCCAAAA TACAAGAACT GAAATTCCAG
    TGTATTGTCT TCTTAAATAT CCCCAGGTAC TGTGCTGGCA CAATGCCCTG GGGTAATCCC
    AGTGACCACA GAGACTTTGA GCCCCAGCGG CACGATGATG GTTATATAGA GGTCATCGGC
    TTTACTATGG CTTCTCTGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGATC TCCATAAAGT GCACTCGGCC
    GACCACCGAC GCGTCTCCGC TCCCCCGGAC TCCTTCTCCG TCCCCCATTC AGTGCCGGAT
    CGCCTGCGTA TCAGAGTGAA CAGGATCCGC CTGCAGGAAT ACGAGGGGCT CCATTATGAA
    AAGGACAAAC TCCGAGACGC CTCTATTCCC CTGGGGATCA TTGTGGTACG AGGAGACTGT
    GACCTAGAGA CCTGCAGGAT GTATATCGAC CGCCTCCAAG AGGATTTACA TTCTGTGACA
    CCCCACCGAG ATCACTGCCA GGATCACGAT AGCTCCTTCC CGCGGTTGCT CTCTGCTCAG
    CGACTCAACC CTCGTTGGTG CTTCTTAGAT GCTACCTCCG CCGACCGGTT TTACCGCATT
    GACAGATCTC AGGAACATCT GCATTTTGTC ACTGAGATTT CCCAAGATGA GATCTTCATT
    CTTGACCCTG AAGCCGATGG GACCCCCCAA GGCAGCACTG GGATGGGAAT GCCAGATCTT
    GTGGTGGAGC AGCCGTCTGG GCCATCGGAA TGGTGGACCC CGACGTCCCG TAAGCGGATA
    GTGAGTGACA GCTGTGTGGC GGGAAGGATG TCTCTCCAAT ATGAAATCTC AGACAAAGTA
    CACGATTTGT CCCCATGTAG CGCCCGCCTG TCACCGGTCT CTGTGGATGA ACAAGCACTC
    ACAGAAGCAG TTGCCGCGGG TGATCTTGCC AAGGTCACGG AGTGTTGCAA GAAGGGAATG
    AGTCTGCGCA TCCCGGGGCC CGAGAGGGGC TCTCTGCTCC ACCTGGCGGC ACGGGCCGGG
    CACAGCGACA TAGTGGCGTA CATCCTACAG AATGGGCCCA CAGATTTGCT GGACATGTCA
    GACAGAGCCA CCGGAGAGAC GGCGCTGCAC AAAGCTGCTT CCCACGGCCA CCGGCGGGTT
    TGCCAAATCC TAGTAGATGC CGGTGCCTGC GTGATAAAGA AAGACGCCAA GGGCAACACT
    CCTTTCGACA AAGCTCAGGA GGCAGGGGAC TCGGAACTGG CATCGTACTT GGACACTTGT
    CAAGCCAAGC AACTTCTTTC CAACGAAGAC CTCGAGACCG CGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754398.1
    CDS2327..5614
    Translation

    Target ORF information:

    RefSeq Version XM_031898538.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTT AATACAAAAA ATTCCACAAG AAGGAAGAAG
    AGGACTTCAT TCAAAAGGAG AGCCAGCAAA AAAGGTTTGG AGGATATTAA AGGCAGGGCC
    TTTATAATAA AACCAATCTC TTCCCCACTC ATGAAACCAT TGCTGGTCTT CGTGAACCCA
    AAAAGTGGAG GGAACCAGGG TACGAAAGTT CTCCAGATGT TTATGTGGTA CTTGAATCCT
    CGGCAGGTCT TTGACCTCTC CCAGGAAGGA CCGCGGGGAG CGCTGGAGAT GTACAGAAAA
    GTGCCAAATC TTCGTATCCT TGCCTGCGGC GGTGACGGGA CGGTGGGCTG GATCCTTTCT
    GTGTTAGATG AGCTTCAGCT GAACCCTCAG CCTCCTGTAG CAGTTTTGCC CCTCGGCACC
    GGGAATGACC TTGCTCGCAC CCTTAACTGG GGAGGGGGTT ACACCGACGA ACCCGTGTCG
    AAGATCCTGT GCCACGTAGA AGACGGCACC AACGTCCAAT TGGATCGCTG GAACCTGCAC
    GTGGAGCGGA ACCCCGACTT ACTCCACGAG GAACTGGATG ATGGGACACA TAAGCTTCCA
    CTGAACGTAT TCAATAACTA CTTCAGCCTG GGGTTCGACG CGCACGTCAC TCTGGAATTC
    CACGAATCAC GAGAAGCAAA TCCCGAGAAA TTCAACAGTC GATTTCGTAA TAAGATGTTC
    TATGCAGGGG CTGCTTTCTC TGATTTCTTG CAAAGAAGCT CCCGGGACTT ATCAAAGCAC
    GTGAAGGTTG TGTGCGACGG CACCGACCTC ACCCCCAAAA TACAAGAACT GAAATTCCAG
    TGTATTGTCT TCTTAAATAT CCCCAGGTAC TGTGCTGGCA CAATGCCCTG GGGTAATCCC
    AGTGACCACA GAGACTTTGA GCCCCAGCGG CACGATGATG GTTATATAGA GGTCATCGGC
    TTTACTATGG CTTCTCTGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGATC TCCATAAAGT GCACTCGGCC
    GACCACCGAC GCGTCTCCGC TCCCCCGGAC TCCTTCTCCG TCCCCCATTC AGTGCCGGAT
    CGCCTGCGTA TCAGAGTGAA CAGGATCCGC CTGCAGGAAT ACGAGGGGCT CCATTATGAA
    AAGGACAAAC TCCGAGACGC CTCTATTCCC CTGGGGATCA TTGTGGTACG AGGAGACTGT
    GACCTAGAGA CCTGCAGGAT GTATATCGAC CGCCTCCAAG AGGATTTACA TTCTGTGACA
    CCCCACCGAG ATCACTGCCA GGATCACGAT AGCTCCTTCC CGCGGTTGCT CTCTGCTCAG
    CGACTCAACC CTCGTTGGTG CTTCTTAGAT GCTACCTCCG CCGACCGGTT TTACCGCATT
    GACAGATCTC AGGAACATCT GCATTTTGTC ACTGAGATTT CCCAAGATGA GATCTTCATT
    CTTGACCCTG AAGCCGATGG GACCCCCCAA GGCAGCACTG GGATGGGAAT GCCAGATCTT
    GTGGTGGAGC AGCCGTCTGG GCCATCGGAA TGGTGGACCC CGACGTCCCG TAAGCGGATA
    GTGAGTGACA GCTGTGTGGC GGGAAGGATG TCTCTCCAAT ATGAAATCTC AGACAAAGTA
    CACGATTTGT CCCCATGTAG CGCCCGCCTG TCACCGGTCT CTGTGGATGA ACAAGCACTC
    ACAGAAGCAG TTGCCGCGGG TGATCTTGCC AAGGTCACGG AGTGTTGCAA GAAGGGAATG
    AGTCTGCGCA TCCCGGGGCC CGAGAGGGGC TCTCTGCTCC ACCTGGCGGC ACGGGCCGGG
    CACAGCGACA TAGTGGCGTA CATCCTACAG AATGGGCCCA CAGATTTGCT GGACATGTCA
    GACAGAGCCA CCGGAGAGAC GGCGCTGCAC AAAGCTGCTT CCCACGGCCA CCGGCGGGTT
    TGCCAAATCC TAGTAGATGC CGGTGCCTGC GTGATAAAGA AAGACGCCAA GGGCAACACT
    CCTTTCGACA AAGCTCAGGA GGCAGGGGAC TCGGAACTGG CATCGTACTT GGACACTTGT
    CAAGCCAAGC AACTTCTTTC CAACGAAGAC CTCGAGACCG CGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54174
    Clone ID Related Accession (Same CDS sequence) XM_031898539.1
    Accession Version XM_031898539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTTAATAC AAAAAATTCC ACAAGAAGGA AGAAGAGGAC TTCATTCAAA
    AGGAGAGCCA GCAAAAAAGG TTTGGAGGAT ATTAAAGGCA GGGCCTTTAT AATAAAACCA
    ATCTCTTCCC CACTCATGAA ACCATTGCTG GTCTTCGTGA ACCCAAAAAG TGGAGGGAAC
    CAGGGTACGA AAGTTCTCCA GATGTTTATG TGGTACTTGA ATCCTCGGCA GGTCTTTGAC
    CTCTCCCAGG AAGGACCGCG GGGAGCGCTG GAGATGTACA GAAAAGTGCC AAATCTTCGT
    ATCCTTGCCT GCGGCGGTGA CGGGACGGTG GGCTGGATCC TTTCTGTGTT AGATGAGCTT
    CAGCTGAACC CTCAGCCTCC TGTAGCAGTT TTGCCCCTCG GCACCGGGAA TGACCTTGCT
    CGCACCCTTA ACTGGGGAGG GGGTTACACC GACGAACCCG TGTCGAAGAT CCTGTGCCAC
    GTAGAAGACG GCACCAACGT CCAATTGGAT CGCTGGAACC TGCACGTGGA GCGGAACCCC
    GACTTACTCC ACGAGGAACT GGATGATGGG ACACATAAGC TTCCACTGAA CGTATTCAAT
    AACTACTTCA GCCTGGGGTT CGACGCGCAC GTCACTCTGG AATTCCACGA ATCACGAGAA
    GCAAATCCCG AGAAATTCAA CAGTCGATTT CGTAATAAGA TGTTCTATGC AGGGGCTGCT
    TTCTCTGATT TCTTGCAAAG AAGCTCCCGG GACTTATCAA AGCACGTGAA GGTTGTGTGC
    GACGGCACCG ACCTCACCCC CAAAATACAA GAACTGAAAT TCCAGTGTAT TGTCTTCTTA
    AATATCCCCA GGTACTGTGC TGGCACAATG CCCTGGGGTA ATCCCAGTGA CCACAGAGAC
    TTTGAGCCCC AGCGGCACGA TGATGGTTAT ATAGAGGTCA TCGGCTTTAC TATGGCTTCT
    CTGGCCGCCC TGCAGGTTGG AGGCCACGGA GAGAGGCTGC ACCAGTGCAG GGAGGTGACA
    TTGTTGACAT ACAAAGCCAT CCCTATGCAG GTCGATGGAG AACCATGTCG GCTTGCACCT
    TCCCTCATCC GCATATCCCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGCGGAGG
    ACTTCCATGC CCTTACTTAA TGATCTCCAT AAAGTGCACT CGGCCGACCA CCGACGCGTC
    TCCGCTCCCC CGGACTCCTT CTCCGTCCCC CATTCAGTGC CGGATCGCCT GCGTATCAGA
    GTGAACAGGA TCCGCCTGCA GGAATACGAG GGGCTCCATT ATGAAAAGGA CAAACTCCGA
    GACGCCTCTA TTCCCCTGGG GATCATTGTG GTACGAGGAG ACTGTGACCT AGAGACCTGC
    AGGATGTATA TCGACCGCCT CCAAGAGGAT TTACATTCTG TGACACCCCA CCGAGATCAC
    TGCCAGGATC ACGATAGCTC CTTCCCGCGG TTGCTCTCTG CTCAGCGACT CAACCCTCGT
    TGGTGCTTCT TAGATGCTAC CTCCGCCGAC CGGTTTTACC GCATTGACAG ATCTCAGGAA
    CATCTGCATT TTGTCACTGA GATTTCCCAA GATGAGATCT TCATTCTTGA CCCTGAAGCC
    GATGGGACCC CCCAAGGCAG CACTGGGATG GGAATGCCAG ATCTTGTGGT GGAGCAGCCG
    TCTGGGCCAT CGGAATGGTG GACCCCGACG TCCCGTAAGC GGATAGTGAG TGACAGCTGT
    GTGGCGGGAA GGATGTCTCT CCAATATGAA ATCTCAGACA AAGTACACGA TTTGTCCCCA
    TGTAGCGCCC GCCTGTCACC GGTCTCTGTG GATGAACAAG CACTCACAGA AGCAGTTGCC
    GCGGGTGATC TTGCCAAGGT CACGGAGTGT TGCAAGAAGG GAATGAGTCT GCGCATCCCG
    GGGCCCGAGA GGGGCTCTCT GCTCCACCTG GCGGCACGGG CCGGGCACAG CGACATAGTG
    GCGTACATCC TACAGAATGG GCCCACAGAT TTGCTGGACA TGTCAGACAG AGCCACCGGA
    GAGACGGCGC TGCACAAAGC TGCTTCCCAC GGCCACCGGC GGGTTTGCCA AATCCTAGTA
    GATGCCGGTG CCTGCGTGAT AAAGAAAGAC GCCAAGGGCA ACACTCCTTT CGACAAAGCT
    CAGGAGGCAG GGGACTCGGA ACTGGCATCG TACTTGGACA CTTGTCAAGC CAAGCAACTT
    CTTTCCAACG AAGACCTCGA GACCGCGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754399.1
    CDS2327..5599
    Translation

    Target ORF information:

    RefSeq Version XM_031898539.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTTAATAC AAAAAATTCC ACAAGAAGGA AGAAGAGGAC TTCATTCAAA
    AGGAGAGCCA GCAAAAAAGG TTTGGAGGAT ATTAAAGGCA GGGCCTTTAT AATAAAACCA
    ATCTCTTCCC CACTCATGAA ACCATTGCTG GTCTTCGTGA ACCCAAAAAG TGGAGGGAAC
    CAGGGTACGA AAGTTCTCCA GATGTTTATG TGGTACTTGA ATCCTCGGCA GGTCTTTGAC
    CTCTCCCAGG AAGGACCGCG GGGAGCGCTG GAGATGTACA GAAAAGTGCC AAATCTTCGT
    ATCCTTGCCT GCGGCGGTGA CGGGACGGTG GGCTGGATCC TTTCTGTGTT AGATGAGCTT
    CAGCTGAACC CTCAGCCTCC TGTAGCAGTT TTGCCCCTCG GCACCGGGAA TGACCTTGCT
    CGCACCCTTA ACTGGGGAGG GGGTTACACC GACGAACCCG TGTCGAAGAT CCTGTGCCAC
    GTAGAAGACG GCACCAACGT CCAATTGGAT CGCTGGAACC TGCACGTGGA GCGGAACCCC
    GACTTACTCC ACGAGGAACT GGATGATGGG ACACATAAGC TTCCACTGAA CGTATTCAAT
    AACTACTTCA GCCTGGGGTT CGACGCGCAC GTCACTCTGG AATTCCACGA ATCACGAGAA
    GCAAATCCCG AGAAATTCAA CAGTCGATTT CGTAATAAGA TGTTCTATGC AGGGGCTGCT
    TTCTCTGATT TCTTGCAAAG AAGCTCCCGG GACTTATCAA AGCACGTGAA GGTTGTGTGC
    GACGGCACCG ACCTCACCCC CAAAATACAA GAACTGAAAT TCCAGTGTAT TGTCTTCTTA
    AATATCCCCA GGTACTGTGC TGGCACAATG CCCTGGGGTA ATCCCAGTGA CCACAGAGAC
    TTTGAGCCCC AGCGGCACGA TGATGGTTAT ATAGAGGTCA TCGGCTTTAC TATGGCTTCT
    CTGGCCGCCC TGCAGGTTGG AGGCCACGGA GAGAGGCTGC ACCAGTGCAG GGAGGTGACA
    TTGTTGACAT ACAAAGCCAT CCCTATGCAG GTCGATGGAG AACCATGTCG GCTTGCACCT
    TCCCTCATCC GCATATCCCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGCGGAGG
    ACTTCCATGC CCTTACTTAA TGATCTCCAT AAAGTGCACT CGGCCGACCA CCGACGCGTC
    TCCGCTCCCC CGGACTCCTT CTCCGTCCCC CATTCAGTGC CGGATCGCCT GCGTATCAGA
    GTGAACAGGA TCCGCCTGCA GGAATACGAG GGGCTCCATT ATGAAAAGGA CAAACTCCGA
    GACGCCTCTA TTCCCCTGGG GATCATTGTG GTACGAGGAG ACTGTGACCT AGAGACCTGC
    AGGATGTATA TCGACCGCCT CCAAGAGGAT TTACATTCTG TGACACCCCA CCGAGATCAC
    TGCCAGGATC ACGATAGCTC CTTCCCGCGG TTGCTCTCTG CTCAGCGACT CAACCCTCGT
    TGGTGCTTCT TAGATGCTAC CTCCGCCGAC CGGTTTTACC GCATTGACAG ATCTCAGGAA
    CATCTGCATT TTGTCACTGA GATTTCCCAA GATGAGATCT TCATTCTTGA CCCTGAAGCC
    GATGGGACCC CCCAAGGCAG CACTGGGATG GGAATGCCAG ATCTTGTGGT GGAGCAGCCG
    TCTGGGCCAT CGGAATGGTG GACCCCGACG TCCCGTAAGC GGATAGTGAG TGACAGCTGT
    GTGGCGGGAA GGATGTCTCT CCAATATGAA ATCTCAGACA AAGTACACGA TTTGTCCCCA
    TGTAGCGCCC GCCTGTCACC GGTCTCTGTG GATGAACAAG CACTCACAGA AGCAGTTGCC
    GCGGGTGATC TTGCCAAGGT CACGGAGTGT TGCAAGAAGG GAATGAGTCT GCGCATCCCG
    GGGCCCGAGA GGGGCTCTCT GCTCCACCTG GCGGCACGGG CCGGGCACAG CGACATAGTG
    GCGTACATCC TACAGAATGG GCCCACAGAT TTGCTGGACA TGTCAGACAG AGCCACCGGA
    GAGACGGCGC TGCACAAAGC TGCTTCCCAC GGCCACCGGC GGGTTTGCCA AATCCTAGTA
    GATGCCGGTG CCTGCGTGAT AAAGAAAGAC GCCAAGGGCA ACACTCCTTT CGACAAAGCT
    CAGGAGGCAG GGGACTCGGA ACTGGCATCG TACTTGGACA CTTGTCAAGC CAAGCAACTT
    CTTTCCAACG AAGACCTCGA GACCGCGGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54175
    Clone ID Related Accession (Same CDS sequence) XM_031898540.1
    Accession Version XM_031898540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTGAAGAA ACCTCAGGTT
    AATACAAAAA ATTCCACAAG AAGGAAGAAG AGGACTTCAT TCAAAAGGAG AGCCAGCAAA
    AAAGGTTTGG AGGATATTAA AGGCAGGGCC TTTATAATAA AACCAATCTC TTCCCCACTC
    ATGAAACCAT TGCTGGTCTT CGTGAACCCA AAAAGTGGAG GGAACCAGGG TACGAAAGTT
    CTCCAGATGT TTATGTGGTA CTTGAATCCT CGGCAGGTCT TTGACCTCTC CCAGGAAGGA
    CCGCGGGGAG CGCTGGAGAT GTACAGAAAA GTGCCAAATC TTCGTATCCT TGCCTGCGGC
    GGTGACGGGA CGGTGGGCTG GATCCTTTCT GTGTTAGATG AGCTTCAGCT GAACCCTCAG
    CCTCCTGTAG CAGTTTTGCC CCTCGGCACC GGGAATGACC TTGCTCGCAC CCTTAACTGG
    GGAGGGGGTT ACACCGACGA ACCCGTGTCG AAGATCCTGT GCCACGTAGA AGACGGCACC
    AACGTCCAAT TGGATCGCTG GAACCTGCAC GTGGAGCGGA ACCCCGACTT ACTCCACGAG
    GAACTGGATG ATGGGACACA TAAGCTTCCA CTGAACGTAT TCAATAACTA CTTCAGCCTG
    GGGTTCGACG CGCACGTCAC TCTGGAATTC CACGAATCAC GAGAAGCAAA TCCCGAGAAA
    TTCAACAGTC GATTTCGTAA TAAGATGTTC TATGCAGGGG CTGCTTTCTC TGATTTCTTG
    CAAAGAAGCT CCCGGGACTT ATCAAAGCAC GTGAAGGTTG TGTGCGACGG CACCGACCTC
    ACCCCCAAAA TACAAGAACT GAAATTCCAG TGTATTGTCT TCTTAAATAT CCCCAGGTAC
    TGTGCTGGCA CAATGCCCTG GGGTAATCCC AGTGACCACA GAGACTTTGA GCCCCAGCGG
    CACGATGATG GTTATATAGA GGTCATCGGC TTTACTATGG CTTCTCTGGC CGCCCTGCAG
    GTTGGAGGCC ACGGAGAGAG GCTGCACCAG TGCAGGGAGG TGACATTGTT GACATACAAA
    GCCATCCCTA TGCAGGTCGA TGGAGAACCA TGTCGGCTTG CACCTTCCCT CATCCGCATA
    TCCCTGAGGA ACCAGGCCAA CATGGTGCAG AAGAGCAAGC GGAGGACTTC CATGCCCTTA
    CTTAATGATC TCCATAAAGT GCACTCGGCC GACCACCGAC GCGTCTCCGC TCCCCCGGAC
    TCCTTCTCCG TCCCCCATTC AGTGCCGGAT CGCCTGCGTA TCAGAGTGAA CAGGATCCGC
    CTGCAGGAAT ACGAGGGGCT CCATTATGAA AAGGACAAAC TCCGAGACGC CTCTATTCCC
    CTGGGGATCA TTGTGGTACG AGGAGACTGT GACCTAGAGA CCTGCAGGAT GTATATCGAC
    CGCCTCCAAG AGGATTTACA TTCTGTGACA CCCCACCGAG ATCACTGCCA GGATCACGAT
    AGCTCCTTCC CGCGGTTGCT CTCTGCTCAG CGACTCAACC CTCGTTGGTG CTTCTTAGAT
    GCTACCTCCG CCGACCGGTT TTACCGCATT GACAGATCTC AGGAACATCT GCATTTTGTC
    ACTGAGATTT CCCAAGATGA GATCTTCATT CTTGACCCTG AAGCCGATGG GACCCCCCAA
    GGCAGCACTG GGATGGGAAT GCCAGATCTT GTGGTGGAGC AGCCGTCTGG GCCATCGGAA
    TGGTGGACCC CGACGTCCCG TAAGCGGATA GTGAGTGACA GCTGTGTGGC GGGAAGGATG
    TCTCTCCAAT ATGAAATCTC AGACAAAGTA CACGATTTGT CCCCATGTAG CGCCCGCCTG
    TCACCGGTCT CTGTGGATGA ACAAGCACTC ACAGAAGCAG TTGCCGCGGG TGATCTTGCC
    AAGGTCACGG AGTGTTGCAA GAAGGGAATG AGTCTGCGCA TCCCGGGGCC CGAGAGGGGC
    TCTCTGCTCC ACCTGGCGGC ACGGGCCGGG CACAGCGACA TAGTGGCGTA CATCCTACAG
    AATGGGCCCA CAGATTTGCT GGACATGTCA GACAGAGCCA CCGGAGAGAC GGCGCTGCAC
    AAAGCTGCTT CCCACGGCCA CCGGCGGGTT TGCCAAATCC TAGTAGATGC CGGTGCCTGC
    GTGATAAAGA AAGACGCCAA GGGCAACACT CCTTTCGACA AAGCTCAGGA GGCAGGGGAC
    TCGGAACTGG CATCGTACTT GGACACTTGT CAAGCCAAGC AACTTCTTTC CAACGAAGAC
    CTCGAGACCG CGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754400.1
    CDS2327..5584
    Translation

    Target ORF information:

    RefSeq Version XM_031898540.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTGAAGAA ACCTCAGGTT
    AATACAAAAA ATTCCACAAG AAGGAAGAAG AGGACTTCAT TCAAAAGGAG AGCCAGCAAA
    AAAGGTTTGG AGGATATTAA AGGCAGGGCC TTTATAATAA AACCAATCTC TTCCCCACTC
    ATGAAACCAT TGCTGGTCTT CGTGAACCCA AAAAGTGGAG GGAACCAGGG TACGAAAGTT
    CTCCAGATGT TTATGTGGTA CTTGAATCCT CGGCAGGTCT TTGACCTCTC CCAGGAAGGA
    CCGCGGGGAG CGCTGGAGAT GTACAGAAAA GTGCCAAATC TTCGTATCCT TGCCTGCGGC
    GGTGACGGGA CGGTGGGCTG GATCCTTTCT GTGTTAGATG AGCTTCAGCT GAACCCTCAG
    CCTCCTGTAG CAGTTTTGCC CCTCGGCACC GGGAATGACC TTGCTCGCAC CCTTAACTGG
    GGAGGGGGTT ACACCGACGA ACCCGTGTCG AAGATCCTGT GCCACGTAGA AGACGGCACC
    AACGTCCAAT TGGATCGCTG GAACCTGCAC GTGGAGCGGA ACCCCGACTT ACTCCACGAG
    GAACTGGATG ATGGGACACA TAAGCTTCCA CTGAACGTAT TCAATAACTA CTTCAGCCTG
    GGGTTCGACG CGCACGTCAC TCTGGAATTC CACGAATCAC GAGAAGCAAA TCCCGAGAAA
    TTCAACAGTC GATTTCGTAA TAAGATGTTC TATGCAGGGG CTGCTTTCTC TGATTTCTTG
    CAAAGAAGCT CCCGGGACTT ATCAAAGCAC GTGAAGGTTG TGTGCGACGG CACCGACCTC
    ACCCCCAAAA TACAAGAACT GAAATTCCAG TGTATTGTCT TCTTAAATAT CCCCAGGTAC
    TGTGCTGGCA CAATGCCCTG GGGTAATCCC AGTGACCACA GAGACTTTGA GCCCCAGCGG
    CACGATGATG GTTATATAGA GGTCATCGGC TTTACTATGG CTTCTCTGGC CGCCCTGCAG
    GTTGGAGGCC ACGGAGAGAG GCTGCACCAG TGCAGGGAGG TGACATTGTT GACATACAAA
    GCCATCCCTA TGCAGGTCGA TGGAGAACCA TGTCGGCTTG CACCTTCCCT CATCCGCATA
    TCCCTGAGGA ACCAGGCCAA CATGGTGCAG AAGAGCAAGC GGAGGACTTC CATGCCCTTA
    CTTAATGATC TCCATAAAGT GCACTCGGCC GACCACCGAC GCGTCTCCGC TCCCCCGGAC
    TCCTTCTCCG TCCCCCATTC AGTGCCGGAT CGCCTGCGTA TCAGAGTGAA CAGGATCCGC
    CTGCAGGAAT ACGAGGGGCT CCATTATGAA AAGGACAAAC TCCGAGACGC CTCTATTCCC
    CTGGGGATCA TTGTGGTACG AGGAGACTGT GACCTAGAGA CCTGCAGGAT GTATATCGAC
    CGCCTCCAAG AGGATTTACA TTCTGTGACA CCCCACCGAG ATCACTGCCA GGATCACGAT
    AGCTCCTTCC CGCGGTTGCT CTCTGCTCAG CGACTCAACC CTCGTTGGTG CTTCTTAGAT
    GCTACCTCCG CCGACCGGTT TTACCGCATT GACAGATCTC AGGAACATCT GCATTTTGTC
    ACTGAGATTT CCCAAGATGA GATCTTCATT CTTGACCCTG AAGCCGATGG GACCCCCCAA
    GGCAGCACTG GGATGGGAAT GCCAGATCTT GTGGTGGAGC AGCCGTCTGG GCCATCGGAA
    TGGTGGACCC CGACGTCCCG TAAGCGGATA GTGAGTGACA GCTGTGTGGC GGGAAGGATG
    TCTCTCCAAT ATGAAATCTC AGACAAAGTA CACGATTTGT CCCCATGTAG CGCCCGCCTG
    TCACCGGTCT CTGTGGATGA ACAAGCACTC ACAGAAGCAG TTGCCGCGGG TGATCTTGCC
    AAGGTCACGG AGTGTTGCAA GAAGGGAATG AGTCTGCGCA TCCCGGGGCC CGAGAGGGGC
    TCTCTGCTCC ACCTGGCGGC ACGGGCCGGG CACAGCGACA TAGTGGCGTA CATCCTACAG
    AATGGGCCCA CAGATTTGCT GGACATGTCA GACAGAGCCA CCGGAGAGAC GGCGCTGCAC
    AAAGCTGCTT CCCACGGCCA CCGGCGGGTT TGCCAAATCC TAGTAGATGC CGGTGCCTGC
    GTGATAAAGA AAGACGCCAA GGGCAACACT CCTTTCGACA AAGCTCAGGA GGCAGGGGAC
    TCGGAACTGG CATCGTACTT GGACACTTGT CAAGCCAAGC AACTTCTTTC CAACGAAGAC
    CTCGAGACCG CGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54176
    Clone ID Related Accession (Same CDS sequence) XM_031898541.1
    Accession Version XM_031898541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGA ATCTGGTCAG ACACCACTGG
    GTCCATCGGC GGAGGCAGGA GGGCAAATGC AAGCAGTGTG GGAAGGGGTT CCAGCAGAAG
    TTTTCATTCC ATAGCAAGGA GATTGTGGCA ATTAGCTGTT CCTGGTGCAA ACAGGCGTTC
    CACAACAAGG TCACTTGCTT CCTGCTCCAT CACATAGAGG AGCCCTGCTC ATTGGGGGCC
    CATGCAGCAG TCATAGTCCC TCCAAGCTGG ATCATTAAGG TGAAGAAACC TCAGGTTAAT
    ACAAAAAATT CCACAAGAAG GAAGAAGAGG ACTTCATTCA AAAGGAGAGC CAGCAAAAAA
    GGTTTGGAGG ATATTAAAGG CAGGGCCTTT ATAATAAAAC CAATCTCTTC CCCACTCATG
    AAACCATTGC TGGTCTTCGT GAACCCAAAA AGTGGAGGGA ACCAGGGTAC GAAAGTTCTC
    CAGATGTTTA TGTGGTACTT GAATCCTCGG CAGGTCTTTG ACCTCTCCCA GGAAGGACCG
    CGGGGAGCGC TGGAGATGTA CAGAAAAGTG CCAAATCTTC GTATCCTTGC CTGCGGCGGT
    GACGGGACGG TGGGCTGGAT CCTTTCTGTG TTAGATGAGC TTCAGCTGAA CCCTCAGCCT
    CCTGTAGCAG TTTTGCCCCT CGGCACCGGG AATGACCTTG CTCGCACCCT TAACTGGGGA
    GGGGGTTACA CCGACGAACC CGTGTCGAAG ATCCTGTGCC ACGTAGAAGA CGGCACCAAC
    GTCCAATTGG ATCGCTGGAA CCTGCACGTG GAGCGGAACC CCGACTTACT CCACGAGGAA
    CTGGATGATG GGACACATAA GCTTCCACTG AACGTATTCA ATAACTACTT CAGCCTGGGG
    TTCGACGCGC ACGTCACTCT GGAATTCCAC GAATCACGAG AAGCAAATCC CGAGAAATTC
    AACAGTCGAT TTCGTAATAA GATGTTCTAT GCAGGGGCTG CTTTCTCTGA TTTCTTGCAA
    AGAAGCTCCC GGGACTTATC AAAGCACGTG AAGGTTGTGT GCGACGGCAC CGACCTCACC
    CCCAAAATAC AAGAACTGAA ATTCCAGTGT ATTGTCTTCT TAAATATCCC CAGGTACTGT
    GCTGGCACAA TGCCCTGGGG TAATCCCAGT GACCACAGAG ACTTTGAGCC CCAGCGGCAC
    GATGATGGTT ATATAGAGGT CATCGGCTTT ACTATGGCTT CTCTGGCCGC CCTGCAGGTT
    GGAGGCCACG GAGAGAGGCT GCACCAGTGC AGGGAGGTGA CATTGTTGAC ATACAAAGCC
    ATCCCTATGC AGGTCGATGG AGAACCATGT CGGCTTGCAC CTTCCCTCAT CCGCATATCC
    CTGAGGAACC AGGCCAACAT GGTGCAGAAG AGCAAGCGGA GGACTTCCAT GCCCTTACTT
    AATGATCTCC ATAAAGTGCA CTCGGCCGAC CACCGACGCG TCTCCGCTCC CCCGGACTCC
    TTCTCCGTCC CCCATTCAGT GCCGGATCGC CTGCGTATCA GAGTGAACAG GATCCGCCTG
    CAGGAATACG AGGGGCTCCA TTATGAAAAG GACAAACTCC GAGACGCCTC TATTCCCCTG
    GGGATCATTG TGGTACGAGG AGACTGTGAC CTAGAGACCT GCAGGATGTA TATCGACCGC
    CTCCAAGAGG ATTTACATTC TGTGACACCC CACCGAGATC ACTGCCAGGA TCACGATAGC
    TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA CTCAACCCTC GTTGGTGCTT CTTAGATGCT
    ACCTCCGCCG ACCGGTTTTA CCGCATTGAC AGATCTCAGG AACATCTGCA TTTTGTCACT
    GAGATTTCCC AAGATGAGAT CTTCATTCTT GACCCTGAAG CCGATGGGAC CCCCCAAGGC
    AGCACTGGGA TGGGAATGCC AGATCTTGTG GTGGAGCAGC CGTCTGGGCC ATCGGAATGG
    TGGACCCCGA CGTCCCGTAA GCGGATAGTG AGTGACAGCT GTGTGGCGGG AAGGATGTCT
    CTCCAATATG AAATCTCAGA CAAAGTACAC GATTTGTCCC CATGTAGCGC CCGCCTGTCA
    CCGGTCTCTG TGGATGAACA AGCACTCACA GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG
    GTCACGGAGT GTTGCAAGAA GGGAATGAGT CTGCGCATCC CGGGGCCCGA GAGGGGCTCT
    CTGCTCCACC TGGCGGCACG GGCCGGGCAC AGCGACATAG TGGCGTACAT CCTACAGAAT
    GGGCCCACAG ATTTGCTGGA CATGTCAGAC AGAGCCACCG GAGAGACGGC GCTGCACAAA
    GCTGCTTCCC ACGGCCACCG GCGGGTTTGC CAAATCCTAG TAGATGCCGG TGCCTGCGTG
    ATAAAGAAAG ACGCCAAGGG CAACACTCCT TTCGACAAAG CTCAGGAGGC AGGGGACTCG
    GAACTGGCAT CGTACTTGGA CACTTGTCAA GCCAAGCAAC TTCTTTCCAA CGAAGACCTC
    GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754401.1
    CDS2327..5581
    Translation

    Target ORF information:

    RefSeq Version XM_031898541.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGA ATCTGGTCAG ACACCACTGG
    GTCCATCGGC GGAGGCAGGA GGGCAAATGC AAGCAGTGTG GGAAGGGGTT CCAGCAGAAG
    TTTTCATTCC ATAGCAAGGA GATTGTGGCA ATTAGCTGTT CCTGGTGCAA ACAGGCGTTC
    CACAACAAGG TCACTTGCTT CCTGCTCCAT CACATAGAGG AGCCCTGCTC ATTGGGGGCC
    CATGCAGCAG TCATAGTCCC TCCAAGCTGG ATCATTAAGG TGAAGAAACC TCAGGTTAAT
    ACAAAAAATT CCACAAGAAG GAAGAAGAGG ACTTCATTCA AAAGGAGAGC CAGCAAAAAA
    GGTTTGGAGG ATATTAAAGG CAGGGCCTTT ATAATAAAAC CAATCTCTTC CCCACTCATG
    AAACCATTGC TGGTCTTCGT GAACCCAAAA AGTGGAGGGA ACCAGGGTAC GAAAGTTCTC
    CAGATGTTTA TGTGGTACTT GAATCCTCGG CAGGTCTTTG ACCTCTCCCA GGAAGGACCG
    CGGGGAGCGC TGGAGATGTA CAGAAAAGTG CCAAATCTTC GTATCCTTGC CTGCGGCGGT
    GACGGGACGG TGGGCTGGAT CCTTTCTGTG TTAGATGAGC TTCAGCTGAA CCCTCAGCCT
    CCTGTAGCAG TTTTGCCCCT CGGCACCGGG AATGACCTTG CTCGCACCCT TAACTGGGGA
    GGGGGTTACA CCGACGAACC CGTGTCGAAG ATCCTGTGCC ACGTAGAAGA CGGCACCAAC
    GTCCAATTGG ATCGCTGGAA CCTGCACGTG GAGCGGAACC CCGACTTACT CCACGAGGAA
    CTGGATGATG GGACACATAA GCTTCCACTG AACGTATTCA ATAACTACTT CAGCCTGGGG
    TTCGACGCGC ACGTCACTCT GGAATTCCAC GAATCACGAG AAGCAAATCC CGAGAAATTC
    AACAGTCGAT TTCGTAATAA GATGTTCTAT GCAGGGGCTG CTTTCTCTGA TTTCTTGCAA
    AGAAGCTCCC GGGACTTATC AAAGCACGTG AAGGTTGTGT GCGACGGCAC CGACCTCACC
    CCCAAAATAC AAGAACTGAA ATTCCAGTGT ATTGTCTTCT TAAATATCCC CAGGTACTGT
    GCTGGCACAA TGCCCTGGGG TAATCCCAGT GACCACAGAG ACTTTGAGCC CCAGCGGCAC
    GATGATGGTT ATATAGAGGT CATCGGCTTT ACTATGGCTT CTCTGGCCGC CCTGCAGGTT
    GGAGGCCACG GAGAGAGGCT GCACCAGTGC AGGGAGGTGA CATTGTTGAC ATACAAAGCC
    ATCCCTATGC AGGTCGATGG AGAACCATGT CGGCTTGCAC CTTCCCTCAT CCGCATATCC
    CTGAGGAACC AGGCCAACAT GGTGCAGAAG AGCAAGCGGA GGACTTCCAT GCCCTTACTT
    AATGATCTCC ATAAAGTGCA CTCGGCCGAC CACCGACGCG TCTCCGCTCC CCCGGACTCC
    TTCTCCGTCC CCCATTCAGT GCCGGATCGC CTGCGTATCA GAGTGAACAG GATCCGCCTG
    CAGGAATACG AGGGGCTCCA TTATGAAAAG GACAAACTCC GAGACGCCTC TATTCCCCTG
    GGGATCATTG TGGTACGAGG AGACTGTGAC CTAGAGACCT GCAGGATGTA TATCGACCGC
    CTCCAAGAGG ATTTACATTC TGTGACACCC CACCGAGATC ACTGCCAGGA TCACGATAGC
    TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA CTCAACCCTC GTTGGTGCTT CTTAGATGCT
    ACCTCCGCCG ACCGGTTTTA CCGCATTGAC AGATCTCAGG AACATCTGCA TTTTGTCACT
    GAGATTTCCC AAGATGAGAT CTTCATTCTT GACCCTGAAG CCGATGGGAC CCCCCAAGGC
    AGCACTGGGA TGGGAATGCC AGATCTTGTG GTGGAGCAGC CGTCTGGGCC ATCGGAATGG
    TGGACCCCGA CGTCCCGTAA GCGGATAGTG AGTGACAGCT GTGTGGCGGG AAGGATGTCT
    CTCCAATATG AAATCTCAGA CAAAGTACAC GATTTGTCCC CATGTAGCGC CCGCCTGTCA
    CCGGTCTCTG TGGATGAACA AGCACTCACA GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG
    GTCACGGAGT GTTGCAAGAA GGGAATGAGT CTGCGCATCC CGGGGCCCGA GAGGGGCTCT
    CTGCTCCACC TGGCGGCACG GGCCGGGCAC AGCGACATAG TGGCGTACAT CCTACAGAAT
    GGGCCCACAG ATTTGCTGGA CATGTCAGAC AGAGCCACCG GAGAGACGGC GCTGCACAAA
    GCTGCTTCCC ACGGCCACCG GCGGGTTTGC CAAATCCTAG TAGATGCCGG TGCCTGCGTG
    ATAAAGAAAG ACGCCAAGGG CAACACTCCT TTCGACAAAG CTCAGGAGGC AGGGGACTCG
    GAACTGGCAT CGTACTTGGA CACTTGTCAA GCCAAGCAAC TTCTTTCCAA CGAAGACCTC
    GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54177
    Clone ID Related Accession (Same CDS sequence) XM_031898542.1
    Accession Version XM_031898542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTTAATAC AAAAAATTCC
    ACAAGAAGGA AGAAGAGGAC TTCATTCAAA AGGAGAGCCA GCAAAAAAGG TTTGGAGGAT
    ATTAAAGGCA GGGCCTTTAT AATAAAACCA ATCTCTTCCC CACTCATGAA ACCATTGCTG
    GTCTTCGTGA ACCCAAAAAG TGGAGGGAAC CAGGGTACGA AAGTTCTCCA GATGTTTATG
    TGGTACTTGA ATCCTCGGCA GGTCTTTGAC CTCTCCCAGG AAGGACCGCG GGGAGCGCTG
    GAGATGTACA GAAAAGTGCC AAATCTTCGT ATCCTTGCCT GCGGCGGTGA CGGGACGGTG
    GGCTGGATCC TTTCTGTGTT AGATGAGCTT CAGCTGAACC CTCAGCCTCC TGTAGCAGTT
    TTGCCCCTCG GCACCGGGAA TGACCTTGCT CGCACCCTTA ACTGGGGAGG GGGTTACACC
    GACGAACCCG TGTCGAAGAT CCTGTGCCAC GTAGAAGACG GCACCAACGT CCAATTGGAT
    CGCTGGAACC TGCACGTGGA GCGGAACCCC GACTTACTCC ACGAGGAACT GGATGATGGG
    ACACATAAGC TTCCACTGAA CGTATTCAAT AACTACTTCA GCCTGGGGTT CGACGCGCAC
    GTCACTCTGG AATTCCACGA ATCACGAGAA GCAAATCCCG AGAAATTCAA CAGTCGATTT
    CGTAATAAGA TGTTCTATGC AGGGGCTGCT TTCTCTGATT TCTTGCAAAG AAGCTCCCGG
    GACTTATCAA AGCACGTGAA GGTTGTGTGC GACGGCACCG ACCTCACCCC CAAAATACAA
    GAACTGAAAT TCCAGTGTAT TGTCTTCTTA AATATCCCCA GGTACTGTGC TGGCACAATG
    CCCTGGGGTA ATCCCAGTGA CCACAGAGAC TTTGAGCCCC AGCGGCACGA TGATGGTTAT
    ATAGAGGTCA TCGGCTTTAC TATGGCTTCT CTGGCCGCCC TGCAGGTTGG AGGCCACGGA
    GAGAGGCTGC ACCAGTGCAG GGAGGTGACA TTGTTGACAT ACAAAGCCAT CCCTATGCAG
    GTCGATGGAG AACCATGTCG GCTTGCACCT TCCCTCATCC GCATATCCCT GAGGAACCAG
    GCCAACATGG TGCAGAAGAG CAAGCGGAGG ACTTCCATGC CCTTACTTAA TGATCTCCAT
    AAAGTGCACT CGGCCGACCA CCGACGCGTC TCCGCTCCCC CGGACTCCTT CTCCGTCCCC
    CATTCAGTGC CGGATCGCCT GCGTATCAGA GTGAACAGGA TCCGCCTGCA GGAATACGAG
    GGGCTCCATT ATGAAAAGGA CAAACTCCGA GACGCCTCTA TTCCCCTGGG GATCATTGTG
    GTACGAGGAG ACTGTGACCT AGAGACCTGC AGGATGTATA TCGACCGCCT CCAAGAGGAT
    TTACATTCTG TGACACCCCA CCGAGATCAC TGCCAGGATC ACGATAGCTC CTTCCCGCGG
    TTGCTCTCTG CTCAGCGACT CAACCCTCGT TGGTGCTTCT TAGATGCTAC CTCCGCCGAC
    CGGTTTTACC GCATTGACAG ATCTCAGGAA CATCTGCATT TTGTCACTGA GATTTCCCAA
    GATGAGATCT TCATTCTTGA CCCTGAAGCC GATGGGACCC CCCAAGGCAG CACTGGGATG
    GGAATGCCAG ATCTTGTGGT GGAGCAGCCG TCTGGGCCAT CGGAATGGTG GACCCCGACG
    TCCCGTAAGC GGATAGTGAG TGACAGCTGT GTGGCGGGAA GGATGTCTCT CCAATATGAA
    ATCTCAGACA AAGTACACGA TTTGTCCCCA TGTAGCGCCC GCCTGTCACC GGTCTCTGTG
    GATGAACAAG CACTCACAGA AGCAGTTGCC GCGGGTGATC TTGCCAAGGT CACGGAGTGT
    TGCAAGAAGG GAATGAGTCT GCGCATCCCG GGGCCCGAGA GGGGCTCTCT GCTCCACCTG
    GCGGCACGGG CCGGGCACAG CGACATAGTG GCGTACATCC TACAGAATGG GCCCACAGAT
    TTGCTGGACA TGTCAGACAG AGCCACCGGA GAGACGGCGC TGCACAAAGC TGCTTCCCAC
    GGCCACCGGC GGGTTTGCCA AATCCTAGTA GATGCCGGTG CCTGCGTGAT AAAGAAAGAC
    GCCAAGGGCA ACACTCCTTT CGACAAAGCT CAGGAGGCAG GGGACTCGGA ACTGGCATCG
    TACTTGGACA CTTGTCAAGC CAAGCAACTT CTTTCCAACG AAGACCTCGA GACCGCGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754402.1
    CDS2327..5569
    Translation

    Target ORF information:

    RefSeq Version XM_031898542.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTTAATAC AAAAAATTCC
    ACAAGAAGGA AGAAGAGGAC TTCATTCAAA AGGAGAGCCA GCAAAAAAGG TTTGGAGGAT
    ATTAAAGGCA GGGCCTTTAT AATAAAACCA ATCTCTTCCC CACTCATGAA ACCATTGCTG
    GTCTTCGTGA ACCCAAAAAG TGGAGGGAAC CAGGGTACGA AAGTTCTCCA GATGTTTATG
    TGGTACTTGA ATCCTCGGCA GGTCTTTGAC CTCTCCCAGG AAGGACCGCG GGGAGCGCTG
    GAGATGTACA GAAAAGTGCC AAATCTTCGT ATCCTTGCCT GCGGCGGTGA CGGGACGGTG
    GGCTGGATCC TTTCTGTGTT AGATGAGCTT CAGCTGAACC CTCAGCCTCC TGTAGCAGTT
    TTGCCCCTCG GCACCGGGAA TGACCTTGCT CGCACCCTTA ACTGGGGAGG GGGTTACACC
    GACGAACCCG TGTCGAAGAT CCTGTGCCAC GTAGAAGACG GCACCAACGT CCAATTGGAT
    CGCTGGAACC TGCACGTGGA GCGGAACCCC GACTTACTCC ACGAGGAACT GGATGATGGG
    ACACATAAGC TTCCACTGAA CGTATTCAAT AACTACTTCA GCCTGGGGTT CGACGCGCAC
    GTCACTCTGG AATTCCACGA ATCACGAGAA GCAAATCCCG AGAAATTCAA CAGTCGATTT
    CGTAATAAGA TGTTCTATGC AGGGGCTGCT TTCTCTGATT TCTTGCAAAG AAGCTCCCGG
    GACTTATCAA AGCACGTGAA GGTTGTGTGC GACGGCACCG ACCTCACCCC CAAAATACAA
    GAACTGAAAT TCCAGTGTAT TGTCTTCTTA AATATCCCCA GGTACTGTGC TGGCACAATG
    CCCTGGGGTA ATCCCAGTGA CCACAGAGAC TTTGAGCCCC AGCGGCACGA TGATGGTTAT
    ATAGAGGTCA TCGGCTTTAC TATGGCTTCT CTGGCCGCCC TGCAGGTTGG AGGCCACGGA
    GAGAGGCTGC ACCAGTGCAG GGAGGTGACA TTGTTGACAT ACAAAGCCAT CCCTATGCAG
    GTCGATGGAG AACCATGTCG GCTTGCACCT TCCCTCATCC GCATATCCCT GAGGAACCAG
    GCCAACATGG TGCAGAAGAG CAAGCGGAGG ACTTCCATGC CCTTACTTAA TGATCTCCAT
    AAAGTGCACT CGGCCGACCA CCGACGCGTC TCCGCTCCCC CGGACTCCTT CTCCGTCCCC
    CATTCAGTGC CGGATCGCCT GCGTATCAGA GTGAACAGGA TCCGCCTGCA GGAATACGAG
    GGGCTCCATT ATGAAAAGGA CAAACTCCGA GACGCCTCTA TTCCCCTGGG GATCATTGTG
    GTACGAGGAG ACTGTGACCT AGAGACCTGC AGGATGTATA TCGACCGCCT CCAAGAGGAT
    TTACATTCTG TGACACCCCA CCGAGATCAC TGCCAGGATC ACGATAGCTC CTTCCCGCGG
    TTGCTCTCTG CTCAGCGACT CAACCCTCGT TGGTGCTTCT TAGATGCTAC CTCCGCCGAC
    CGGTTTTACC GCATTGACAG ATCTCAGGAA CATCTGCATT TTGTCACTGA GATTTCCCAA
    GATGAGATCT TCATTCTTGA CCCTGAAGCC GATGGGACCC CCCAAGGCAG CACTGGGATG
    GGAATGCCAG ATCTTGTGGT GGAGCAGCCG TCTGGGCCAT CGGAATGGTG GACCCCGACG
    TCCCGTAAGC GGATAGTGAG TGACAGCTGT GTGGCGGGAA GGATGTCTCT CCAATATGAA
    ATCTCAGACA AAGTACACGA TTTGTCCCCA TGTAGCGCCC GCCTGTCACC GGTCTCTGTG
    GATGAACAAG CACTCACAGA AGCAGTTGCC GCGGGTGATC TTGCCAAGGT CACGGAGTGT
    TGCAAGAAGG GAATGAGTCT GCGCATCCCG GGGCCCGAGA GGGGCTCTCT GCTCCACCTG
    GCGGCACGGG CCGGGCACAG CGACATAGTG GCGTACATCC TACAGAATGG GCCCACAGAT
    TTGCTGGACA TGTCAGACAG AGCCACCGGA GAGACGGCGC TGCACAAAGC TGCTTCCCAC
    GGCCACCGGC GGGTTTGCCA AATCCTAGTA GATGCCGGTG CCTGCGTGAT AAAGAAAGAC
    GCCAAGGGCA ACACTCCTTT CGACAAAGCT CAGGAGGCAG GGGACTCGGA ACTGGCATCG
    TACTTGGACA CTTGTCAAGC CAAGCAACTT CTTTCCAACG AAGACCTCGA GACCGCGGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54178
    Clone ID Related Accession (Same CDS sequence) XM_031898543.1
    Accession Version XM_031898543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTT AATACAAAAA ATTCCACAAG AAGGAAGAAG
    AGGACTTCAT TCAAAAGGAG AGCCAGCAAA AAAGGTTTGG AGGATATTAA AGGCAGGGCC
    TTTATAATAA AACCAATCTC TTCCCCACTC ATGAAACCAT TGCTGGTCTT CGTGAACCCA
    AAAAGTGGAG GGAACCAGGG TACGAAAGTT CTCCAGATGT TTATGTGGTA CTTGAATCCT
    CGGCAGGTCT TTGACCTCTC CCAGGAAGGA CCGCGGGGAG CGCTGGAGAT GTACAGAAAA
    GTGCCAAATC TTCGTATCCT TGCCTGCGGC GGTGACGGGA CGGTGGGCTG GATCCTTTCT
    GTGTTAGATG AGCTTCAGCT GAACCCTCAG CCTCCTGTAG CAGTTTTGCC CCTCGGCACC
    GGGAATGACC TTGCTCGCAC CCTTAACTGG GGAGGGGGTT ACACCGACGA ACCCGTGTCG
    AAGATCCTGT GCCACGTAGA AGACGGCACC AACGTCCAAT TGGATCGCTG GAACCTGCAC
    GTGGAGCGGA ACCCCGACTT ACTCCACGAG GAACTGGATG ATGGGACACA TAAGCTTCCA
    CTGAACGTAT TCAATAACTA CTTCAGCCTG GGGTTCGACG CGCACGTCAC TCTGGAATTC
    CACGAATCAC GAGAAGCAAA TCCCGAGAAA TTCAACAGTC GATTTCGTAA TAAGATGTTC
    TATGCAGGGG CTGCTTTCTC TGATTTCTTG CAAAGAAGCT CCCGGGACTT ATCAAAGCAC
    GTGAAGGTTG TGTGCGACGG CACCGACCTC ACCCCCAAAA TACAAGAACT GAAATTCCAG
    TGTATTGTCT TCTTAAATAT CCCCAGGTAC TGTGCTGGCA CAATGCCCTG GGGTAATCCC
    AGTGACCACA GAGACTTTGA GCCCCAGCGG CACGATGATG GTTATATAGA GGTCATCGGC
    TTTACTATGG CTTCTCTGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGACC CCCATTCAGT GCCGGATCGC
    CTGCGTATCA GAGTGAACAG GATCCGCCTG CAGGAATACG AGGGGCTCCA TTATGAAAAG
    GACAAACTCC GAGACGCCTC TATTCCCCTG GGGATCATTG TGGTACGAGG AGACTGTGAC
    CTAGAGACCT GCAGGATGTA TATCGACCGC CTCCAAGAGG ATTTACATTC TGTGACACCC
    CACCGAGATC ACTGCCAGGA TCACGATAGC TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA
    CTCAACCCTC GTTGGTGCTT CTTAGATGCT ACCTCCGCCG ACCGGTTTTA CCGCATTGAC
    AGATCTCAGG AACATCTGCA TTTTGTCACT GAGATTTCCC AAGATGAGAT CTTCATTCTT
    GACCCTGAAG CCGATGGGAC CCCCCAAGGC AGCACTGGGA TGGGAATGCC AGATCTTGTG
    GTGGAGCAGC CGTCTGGGCC ATCGGAATGG TGGACCCCGA CGTCCCGTAA GCGGATAGTG
    AGTGACAGCT GTGTGGCGGG AAGGATGTCT CTCCAATATG AAATCTCAGA CAAAGTACAC
    GATTTGTCCC CATGTAGCGC CCGCCTGTCA CCGGTCTCTG TGGATGAACA AGCACTCACA
    GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG GTCACGGAGT GTTGCAAGAA GGGAATGAGT
    CTGCGCATCC CGGGGCCCGA GAGGGGCTCT CTGCTCCACC TGGCGGCACG GGCCGGGCAC
    AGCGACATAG TGGCGTACAT CCTACAGAAT GGGCCCACAG ATTTGCTGGA CATGTCAGAC
    AGAGCCACCG GAGAGACGGC GCTGCACAAA GCTGCTTCCC ACGGCCACCG GCGGGTTTGC
    CAAATCCTAG TAGATGCCGG TGCCTGCGTG ATAAAGAAAG ACGCCAAGGG CAACACTCCT
    TTCGACAAAG CTCAGGAGGC AGGGGACTCG GAACTGGCAT CGTACTTGGA CACTTGTCAA
    GCCAAGCAAC TTCTTTCCAA CGAAGACCTC GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754403.1
    CDS2325..5549
    Translation

    Target ORF information:

    RefSeq Version XM_031898543.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGG TCAGACATTT TTCTTGTTGT
    TCTAACCAGC AGAATCTGGT CAGACACCAC TGGGTCCATC GGCGGAGGCA GGAGGGCAAA
    TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG AAGTTTTCAT TCCATAGCAA GGAGATTGTG
    GCAATTAGCT GTTCCTGGTG CAAACAGGCG TTCCACAACA AGGTCACTTG CTTCCTGCTC
    CATCACATAG AGGAGCCCTG CTCATTGGGG GCCCATGCAG CAGTCATAGT CCCTCCAAGC
    TGGATCATTA AGGTGAAGAA ACCTCAGGTT AATACAAAAA ATTCCACAAG AAGGAAGAAG
    AGGACTTCAT TCAAAAGGAG AGCCAGCAAA AAAGGTTTGG AGGATATTAA AGGCAGGGCC
    TTTATAATAA AACCAATCTC TTCCCCACTC ATGAAACCAT TGCTGGTCTT CGTGAACCCA
    AAAAGTGGAG GGAACCAGGG TACGAAAGTT CTCCAGATGT TTATGTGGTA CTTGAATCCT
    CGGCAGGTCT TTGACCTCTC CCAGGAAGGA CCGCGGGGAG CGCTGGAGAT GTACAGAAAA
    GTGCCAAATC TTCGTATCCT TGCCTGCGGC GGTGACGGGA CGGTGGGCTG GATCCTTTCT
    GTGTTAGATG AGCTTCAGCT GAACCCTCAG CCTCCTGTAG CAGTTTTGCC CCTCGGCACC
    GGGAATGACC TTGCTCGCAC CCTTAACTGG GGAGGGGGTT ACACCGACGA ACCCGTGTCG
    AAGATCCTGT GCCACGTAGA AGACGGCACC AACGTCCAAT TGGATCGCTG GAACCTGCAC
    GTGGAGCGGA ACCCCGACTT ACTCCACGAG GAACTGGATG ATGGGACACA TAAGCTTCCA
    CTGAACGTAT TCAATAACTA CTTCAGCCTG GGGTTCGACG CGCACGTCAC TCTGGAATTC
    CACGAATCAC GAGAAGCAAA TCCCGAGAAA TTCAACAGTC GATTTCGTAA TAAGATGTTC
    TATGCAGGGG CTGCTTTCTC TGATTTCTTG CAAAGAAGCT CCCGGGACTT ATCAAAGCAC
    GTGAAGGTTG TGTGCGACGG CACCGACCTC ACCCCCAAAA TACAAGAACT GAAATTCCAG
    TGTATTGTCT TCTTAAATAT CCCCAGGTAC TGTGCTGGCA CAATGCCCTG GGGTAATCCC
    AGTGACCACA GAGACTTTGA GCCCCAGCGG CACGATGATG GTTATATAGA GGTCATCGGC
    TTTACTATGG CTTCTCTGGC CGCCCTGCAG GTTGGAGGCC ACGGAGAGAG GCTGCACCAG
    TGCAGGGAGG TGACATTGTT GACATACAAA GCCATCCCTA TGCAGGTCGA TGGAGAACCA
    TGTCGGCTTG CACCTTCCCT CATCCGCATA TCCCTGAGGA ACCAGGCCAA CATGGTGCAG
    AAGAGCAAGC GGAGGACTTC CATGCCCTTA CTTAATGACC CCCATTCAGT GCCGGATCGC
    CTGCGTATCA GAGTGAACAG GATCCGCCTG CAGGAATACG AGGGGCTCCA TTATGAAAAG
    GACAAACTCC GAGACGCCTC TATTCCCCTG GGGATCATTG TGGTACGAGG AGACTGTGAC
    CTAGAGACCT GCAGGATGTA TATCGACCGC CTCCAAGAGG ATTTACATTC TGTGACACCC
    CACCGAGATC ACTGCCAGGA TCACGATAGC TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA
    CTCAACCCTC GTTGGTGCTT CTTAGATGCT ACCTCCGCCG ACCGGTTTTA CCGCATTGAC
    AGATCTCAGG AACATCTGCA TTTTGTCACT GAGATTTCCC AAGATGAGAT CTTCATTCTT
    GACCCTGAAG CCGATGGGAC CCCCCAAGGC AGCACTGGGA TGGGAATGCC AGATCTTGTG
    GTGGAGCAGC CGTCTGGGCC ATCGGAATGG TGGACCCCGA CGTCCCGTAA GCGGATAGTG
    AGTGACAGCT GTGTGGCGGG AAGGATGTCT CTCCAATATG AAATCTCAGA CAAAGTACAC
    GATTTGTCCC CATGTAGCGC CCGCCTGTCA CCGGTCTCTG TGGATGAACA AGCACTCACA
    GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG GTCACGGAGT GTTGCAAGAA GGGAATGAGT
    CTGCGCATCC CGGGGCCCGA GAGGGGCTCT CTGCTCCACC TGGCGGCACG GGCCGGGCAC
    AGCGACATAG TGGCGTACAT CCTACAGAAT GGGCCCACAG ATTTGCTGGA CATGTCAGAC
    AGAGCCACCG GAGAGACGGC GCTGCACAAA GCTGCTTCCC ACGGCCACCG GCGGGTTTGC
    CAAATCCTAG TAGATGCCGG TGCCTGCGTG ATAAAGAAAG ACGCCAAGGG CAACACTCCT
    TTCGACAAAG CTCAGGAGGC AGGGGACTCG GAACTGGCAT CGTACTTGGA CACTTGTCAA
    GCCAAGCAAC TTCTTTCCAA CGAAGACCTC GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54179
    Clone ID Related Accession (Same CDS sequence) XM_031898544.1
    Accession Version XM_031898544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product diacylglycerol kinase iota isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTGAAGAA ACCTCAGGTT
    AATACAAAAA ATTCCACAAG AAGGAAGAAG AGGACTTCAT TCAAAAGGAG AGCCAGCAAA
    AAAGGTTTGG AGGATATTAA AGGCAGGGCC TTTATAATAA AACCAATCTC TTCCCCACTC
    ATGAAACCAT TGCTGGTCTT CGTGAACCCA AAAAGTGGAG GGAACCAGGG TACGAAAGTT
    CTCCAGATGT TTATGTGGTA CTTGAATCCT CGGCAGGTCT TTGACCTCTC CCAGGAAGGA
    CCGCGGGGAG CGCTGGAGAT GTACAGAAAA GTGCCAAATC TTCGTATCCT TGCCTGCGGC
    GGTGACGGGA CGGTGGGCTG GATCCTTTCT GTGTTAGATG AGCTTCAGCT GAACCCTCAG
    CCTCCTGTAG CAGTTTTGCC CCTCGGCACC GGGAATGACC TTGCTCGCAC CCTTAACTGG
    GGAGGGGGTT ACACCGACGA ACCCGTGTCG AAGATCCTGT GCCACGTAGA AGACGGCACC
    AACGTCCAAT TGGATCGCTG GAACCTGCAC GTGGAGCGGA ACCCCGACTT ACTCCACGAG
    GAACTGGATG ATGGGACACA TAAGCTTCCA CTGAACGTAT TCAATAACTA CTTCAGCCTG
    GGGTTCGACG CGCACGTCAC TCTGGAATTC CACGAATCAC GAGAAGCAAA TCCCGAGAAA
    TTCAACAGTC GATTTCGTAA TAAGATGTTC TATGCAGGGG CTGCTTTCTC TGATTTCTTG
    CAAAGAAGCT CCCGGGACTT ATCAAAGCAC GTGAAGGTTG TGTGCGACGG CACCGACCTC
    ACCCCCAAAA TACAAGAACT GAAATTCCAG TGTATTGTCT TCTTAAATAT CCCCAGGTAC
    TGTGCTGGCA CAATGCCCTG GGGTAATCCC AGTGACCACA GAGACTTTGA GCCCCAGCGG
    CACGATGATG GTTATATAGA GGTCATCGGC TTTACTATGG CTTCTCTGGC CGCCCTGCAG
    GTTGGAGGCC ACGGAGAGAG GCTGCACCAG TGCAGGGAGG TGACATTGTT GACATACAAA
    GCCATCCCTA TGCAGGTCGA TGGAGAACCA TGTCGGCTTG CACCTTCCCT CATCCGCATA
    TCCCTGAGGA ACCAGGCCAA CATGGTGCAG AAGAGCAAGC GGAGGACTTC CATGCCCTTA
    CTTAATGACC CCCATTCAGT GCCGGATCGC CTGCGTATCA GAGTGAACAG GATCCGCCTG
    CAGGAATACG AGGGGCTCCA TTATGAAAAG GACAAACTCC GAGACGCCTC TATTCCCCTG
    GGGATCATTG TGGTACGAGG AGACTGTGAC CTAGAGACCT GCAGGATGTA TATCGACCGC
    CTCCAAGAGG ATTTACATTC TGTGACACCC CACCGAGATC ACTGCCAGGA TCACGATAGC
    TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA CTCAACCCTC GTTGGTGCTT CTTAGATGCT
    ACCTCCGCCG ACCGGTTTTA CCGCATTGAC AGATCTCAGG AACATCTGCA TTTTGTCACT
    GAGATTTCCC AAGATGAGAT CTTCATTCTT GACCCTGAAG CCGATGGGAC CCCCCAAGGC
    AGCACTGGGA TGGGAATGCC AGATCTTGTG GTGGAGCAGC CGTCTGGGCC ATCGGAATGG
    TGGACCCCGA CGTCCCGTAA GCGGATAGTG AGTGACAGCT GTGTGGCGGG AAGGATGTCT
    CTCCAATATG AAATCTCAGA CAAAGTACAC GATTTGTCCC CATGTAGCGC CCGCCTGTCA
    CCGGTCTCTG TGGATGAACA AGCACTCACA GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG
    GTCACGGAGT GTTGCAAGAA GGGAATGAGT CTGCGCATCC CGGGGCCCGA GAGGGGCTCT
    CTGCTCCACC TGGCGGCACG GGCCGGGCAC AGCGACATAG TGGCGTACAT CCTACAGAAT
    GGGCCCACAG ATTTGCTGGA CATGTCAGAC AGAGCCACCG GAGAGACGGC GCTGCACAAA
    GCTGCTTCCC ACGGCCACCG GCGGGTTTGC CAAATCCTAG TAGATGCCGG TGCCTGCGTG
    ATAAAGAAAG ACGCCAAGGG CAACACTCCT TTCGACAAAG CTCAGGAGGC AGGGGACTCG
    GAACTGGCAT CGTACTTGGA CACTTGTCAA GCCAAGCAAC TTCTTTCCAA CGAAGACCTC
    GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754404.1
    CDS2325..5519
    Translation

    Target ORF information:

    RefSeq Version XM_031898544.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis diacylglycerol kinase, iota (dgki), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCTACC CTGTCTATAA CATGGAGGGC TTTTTCCATC GTCACTTCTG TAAGTCGGGT 
    CCAGCTAATG CAGACAGGCG ACTGAGCATA ACCGCATCCA AAGTCAGGCG TAAGTCAGTA
    GTTGGAGCCC CGTCGCTGGA GTTATGGACC CAACGGAGGT CATCAGCTCA GATCTACCCA
    AAGCGAAAAA ATGCAGAGAA GAGGAGAAAA TCCAGCACGG CTCCAGTCAG GGTGAACCCT
    CGTTTTTCTG TCATTGGCAC CAAGAGACGC CCGGTAAAGT CTGCCCAGAT CCGAATGCTG
    GAGGAGATAC TGTGGGAGAT ATGGATGGAG CGTAAGTTTC AGCCCCAAAT AGAGCCGCAG
    CCCAATGACC GGGGGGCAGG AGAAGGATCC GGTTCCTCAT CTAGTGCTAG CAGCTCAGAC
    AGTGAGTCTG GGAAAGCGAT TTCTCGATCG GGGCTCCAGC ATCTGGCTCC TGCGCACTGC
    CCTGTCTCCA ATGGGCCGGC CAAAGAACTG AGGATGACCC TGGACTGGAC TGAGAATGCC
    ATTAATGGGG AGCACCTCTG GCAGGAGACA AATGTATCTG GAGACCTGTG TTACCTGGGG
    GAGGAGAACT GCCAAGTCAA ATTCGCTAAG TCGGCAATTC GCAGGAAATG TGCAGCTTGC
    AAAATAGTGG TCCATACGGC CTGCATGGAG CAGCTCGACA AGATTAATTT TAGATGCAAA
    CCCACTTTCC GCGATGGAAC CTCTCGGACC CCGAGGGAGC AGAATCTGGT CAGACACCAC
    TGGGTCCATC GGCGGAGGCA GGAGGGCAAA TGCAAGCAGT GTGGGAAGGG GTTCCAGCAG
    AAGTTTTCAT TCCATAGCAA GGAGATTGTG GCAATTAGCT GTTCCTGGTG CAAACAGGCG
    TTCCACAACA AGGTCACTTG CTTCCTGCTC CATCACATAG AGGAGCCCTG CTCATTGGGG
    GCCCATGCAG CAGTCATAGT CCCTCCAAGC TGGATCATTA AGGTGAAGAA ACCTCAGGTT
    AATACAAAAA ATTCCACAAG AAGGAAGAAG AGGACTTCAT TCAAAAGGAG AGCCAGCAAA
    AAAGGTTTGG AGGATATTAA AGGCAGGGCC TTTATAATAA AACCAATCTC TTCCCCACTC
    ATGAAACCAT TGCTGGTCTT CGTGAACCCA AAAAGTGGAG GGAACCAGGG TACGAAAGTT
    CTCCAGATGT TTATGTGGTA CTTGAATCCT CGGCAGGTCT TTGACCTCTC CCAGGAAGGA
    CCGCGGGGAG CGCTGGAGAT GTACAGAAAA GTGCCAAATC TTCGTATCCT TGCCTGCGGC
    GGTGACGGGA CGGTGGGCTG GATCCTTTCT GTGTTAGATG AGCTTCAGCT GAACCCTCAG
    CCTCCTGTAG CAGTTTTGCC CCTCGGCACC GGGAATGACC TTGCTCGCAC CCTTAACTGG
    GGAGGGGGTT ACACCGACGA ACCCGTGTCG AAGATCCTGT GCCACGTAGA AGACGGCACC
    AACGTCCAAT TGGATCGCTG GAACCTGCAC GTGGAGCGGA ACCCCGACTT ACTCCACGAG
    GAACTGGATG ATGGGACACA TAAGCTTCCA CTGAACGTAT TCAATAACTA CTTCAGCCTG
    GGGTTCGACG CGCACGTCAC TCTGGAATTC CACGAATCAC GAGAAGCAAA TCCCGAGAAA
    TTCAACAGTC GATTTCGTAA TAAGATGTTC TATGCAGGGG CTGCTTTCTC TGATTTCTTG
    CAAAGAAGCT CCCGGGACTT ATCAAAGCAC GTGAAGGTTG TGTGCGACGG CACCGACCTC
    ACCCCCAAAA TACAAGAACT GAAATTCCAG TGTATTGTCT TCTTAAATAT CCCCAGGTAC
    TGTGCTGGCA CAATGCCCTG GGGTAATCCC AGTGACCACA GAGACTTTGA GCCCCAGCGG
    CACGATGATG GTTATATAGA GGTCATCGGC TTTACTATGG CTTCTCTGGC CGCCCTGCAG
    GTTGGAGGCC ACGGAGAGAG GCTGCACCAG TGCAGGGAGG TGACATTGTT GACATACAAA
    GCCATCCCTA TGCAGGTCGA TGGAGAACCA TGTCGGCTTG CACCTTCCCT CATCCGCATA
    TCCCTGAGGA ACCAGGCCAA CATGGTGCAG AAGAGCAAGC GGAGGACTTC CATGCCCTTA
    CTTAATGACC CCCATTCAGT GCCGGATCGC CTGCGTATCA GAGTGAACAG GATCCGCCTG
    CAGGAATACG AGGGGCTCCA TTATGAAAAG GACAAACTCC GAGACGCCTC TATTCCCCTG
    GGGATCATTG TGGTACGAGG AGACTGTGAC CTAGAGACCT GCAGGATGTA TATCGACCGC
    CTCCAAGAGG ATTTACATTC TGTGACACCC CACCGAGATC ACTGCCAGGA TCACGATAGC
    TCCTTCCCGC GGTTGCTCTC TGCTCAGCGA CTCAACCCTC GTTGGTGCTT CTTAGATGCT
    ACCTCCGCCG ACCGGTTTTA CCGCATTGAC AGATCTCAGG AACATCTGCA TTTTGTCACT
    GAGATTTCCC AAGATGAGAT CTTCATTCTT GACCCTGAAG CCGATGGGAC CCCCCAAGGC
    AGCACTGGGA TGGGAATGCC AGATCTTGTG GTGGAGCAGC CGTCTGGGCC ATCGGAATGG
    TGGACCCCGA CGTCCCGTAA GCGGATAGTG AGTGACAGCT GTGTGGCGGG AAGGATGTCT
    CTCCAATATG AAATCTCAGA CAAAGTACAC GATTTGTCCC CATGTAGCGC CCGCCTGTCA
    CCGGTCTCTG TGGATGAACA AGCACTCACA GAAGCAGTTG CCGCGGGTGA TCTTGCCAAG
    GTCACGGAGT GTTGCAAGAA GGGAATGAGT CTGCGCATCC CGGGGCCCGA GAGGGGCTCT
    CTGCTCCACC TGGCGGCACG GGCCGGGCAC AGCGACATAG TGGCGTACAT CCTACAGAAT
    GGGCCCACAG ATTTGCTGGA CATGTCAGAC AGAGCCACCG GAGAGACGGC GCTGCACAAA
    GCTGCTTCCC ACGGCCACCG GCGGGTTTGC CAAATCCTAG TAGATGCCGG TGCCTGCGTG
    ATAAAGAAAG ACGCCAAGGG CAACACTCCT TTCGACAAAG CTCAGGAGGC AGGGGACTCG
    GAACTGGCAT CGTACTTGGA CACTTGTCAA GCCAAGCAAC TTCTTTCCAA CGAAGACCTC
    GAGACCGCGG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.