pde8b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde8b
Entrez Gene ID	100490787
Full Name	phosphodiesterase 8B
General protein information	Preferred Names phosphodiesterase 8B Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004910389.3	XP_004910446.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
XM_002940874.5	XP_002940920.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_002940874.4	XP_002940920.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_012967377.2	XP_012822831.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
XM_004910390.3	XP_004910447.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
XM_004910389.4	XP_004910446.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
XM_004910390.4	XP_004910447.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
XM_012967377.3	XP_012822831.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDE8B	XP_546056.4
Bos taurus (cattle)	PDE8B	XP_003586548.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007163	XP_308604.4
Gallus gallus (chicken)	PDE8B	XP_425218.4
Danio rerio (zebrafish)	pde8b	XP_005170307.1
Drosophila melanogaster (fruit fly)	Pde8	NP_001163274.1
Xenopus tropicalis (tropical clawed frog)	pde8b	XP_002940920.1
Pan troglodytes (chimpanzee)	PDE8B	XP_517695.4
Mus musculus (house mouse)	Pde8b	NP_758467.2
Rattus norvegicus (Norway rat)	Pde8b	NP_954889.1
Homo sapiens (human)	PDE8B	NP_003710.1

Gene Ontology

The following pde8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03336	XM_002940874.5 Latest version!	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03337	XM_004910389.4 Latest version!	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03341	XM_004910390.4 Latest version!	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa34852	XM_012967377.3 Latest version!	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa03336	XM_002940874.4	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa03337	XM_004910389.3	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa03341	XM_004910390.3	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa34852	XM_012967377.2	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde8b ( XM_002940874.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940920.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03336
Clone ID Related Accession (Same CDS sequence)	XM_002940874.5 , XM_002940874.4
Accession Version	XM_002940874.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940874.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGCA GGTATCATCT 
ACAGAACTGT GGCTTGGACC TATGAGACTG ACCCAGGATC CAATCCAGGT GTTGCTAGTC 
TTTGCAAAAG AAGATAGCCA GAGCGATGGC TTCTGGTGGG CCTGTGATCG AGCGGGATAC 
AGATGTAATA TTGCTCGGAC GCCAGAGTCC GCAGTTGAGT GCTTTTTGGA TAAGCACCAT 
GAAATCATTG TCATAGATCA CAGGCATTCA AGATACCTCG ATGCAGAAGC TGTGTGCAGA 
TCAATACGAG CTACAAAACC CTCAGAGCAC ACAACAATTC TGGCTGTTGT TCCACGTGTA 
GCTTCTGATC AAGAGGAGAT GTCTGTGCTA CCACTTCTGA ATGTGGGATT TAATAGGAGA 
TTTGTGGAGA ACAGCAGTAT GATTGCATGT TACAATGAGC TGATCCAGAT AGAACATGGG 
GAGGTGCGAT CTCAGTTCAA ACTGCGGTCC TGTAACTCAA TATTTACAGC CTTAGACAAC 
TGCCAAGAAG CCATAGAAAT AACAAGTGAA GATCATATTA TACAGTATGT TAACCCAGCC 
TTTGAACGGA TGATGGGATA TCATAAGGAA GAACTTATTG GGAAGGAACT TACTGAACTG 
CCAAAAAGTG ACAAAAATCG GGCAGATCTG TTGGACACCA TTAACACGTG CATCAAAAAG 
GGAAAGGAAT GGCAAGGAAT CTATTTTGCA AGAAGGAAAT CAGGAGACAG CATTCAACAG 
CATGTAAAAG TGACCCCAGT AATTGGGCAA GGGGGGAAAA TTAGGCATTT TGTGTCTATC 
AAGAAGCTGT GCAATAGCAA TGACAACAAT AAACAGATCT ACAAGGTAAA TCGAGAAGAC 
AATGCACTCA CAGAGTCTCA CTCGTTTAGA CTTAAGAACA GAAGGAAAGA ATCAGTGGAT 
GTGAGATCTA TCACTTCTCG CAGTAGTGAT GCACCAAGTT TGCAGAACCA CAGATACTCT 
TCAATGGCTC GAATTCACAC AATGACTATT GAGGCTCCAA TAACAAAGGT CATAAACATC 
ATCAACGCAG CCCAGGAGAA CAGCCCCGTG ACTGTAGCCG AAGCTCTAGA CAGAGTTTTG 
GAAATTCTTA GAACAACTGA ACTCTATTCG CCTCAGCTGG GAACCAAAGA TGAAGACCCC 
CACACCAGTG ATCTTGTAGG AGGTTTGATG ACTGATGGCT TAAGAAGGCT TTCAGGAAAT 
GAGTATGTTT TCTCCAAAAA TGTGCACCAC AGCCATAGCC ACCTTTCAGT GCCAATAACC 
ATCAATGACG TCCCACCTTG CATCGCAGAA TTACTAGAGG ATGAAGTTTC ATGGGACTTT 
AACATATTTG AATTAGAAGC TGTTACAATT AAAAGGCCGC TGGTATACCT TGGTTTAAAG 
ATATTTACTT GTTTTGGAGC CTGTGAGTTT TTGAATTGCT CAGAAGTGAC ACTTCGCTCC 
TGGTTACAGT TAGTTGAAGC CAACTATCAC GCATCAAATG CCTACCACAA CTCTACCCAT 
GCTGCCGATG TACTACATGC CACCGCTTTT TTCCTCCAGC AGGAGAATGT TAAGGGAAGC 
CTTGACCAGT TAGACAAAGT GGCGGCACTA ATTGCTGCCA CGGTACATGA TGTGGATCAT 
CCGGGCCGGA CTAATTCCTT CCTCTGTAAT GCCAGCAGCG AATTAGCCGT ATTGTACAAT 
GATACAGCAG TGTTGGAAAG CCATCATGCA GCACTCGCAT TTCAGCTCAC AGCAAAGGAC 
AATAAATGCA ACATCTTCAA GAACATGGAC CGGACTCAGT ATCGGACTCT CCGGCAGGCA 
ATCATTGACA TGGTTTTAGC AACAGAAATG ACCAAGCATT TTGAGCATGT CAACAAGTTC 
GTCAACAGCA TCAACAAACC CATTGCAGCC GAAGATAGCA ATTCCAACAG TGAGGGGAGT 
GATTTTGAAT GTTCCACCAA TATAAGGAAT TCTCCAGAGA ACCGGATCCT CATCAAACGC 
ATGATGATCA AATGCGCTGA CGTAGCCAAC CCCTGCCGCC CCCTGAACCT GTGCATAGAG 
TGGGCTGGAA GGATCTCCGA GGAGTATTTT GCACAGACAG ATGAAGAGAA GAGGCAAGGC 
TTGCCAGTGG TGATGCCAGT CTTTGACCGA AACACCTGCA GCATACCCAA GTCTCAGATC 
TCATTTATAG ACTATTTTAT TACAGACATG TTTGATGCGT GGGATGCCTT CGCCCATTTG 
CCCAACCTCA TCCTCCATCT GACAGACAAT TACAAGTACT GGAAATCATT AGATGAATTG 
AAGTGTAAAA GTCTCAGGTG TTCGTCAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940920.1
CDS	1145..3757
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTAATLHGLFIKTDAADSIPSVLAYQGRYPPAARSGSRKGRRDQGPHSCIEAETQTSHTSVKQVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_002940874.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940874.4

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGCA GGTATCATCT 
ACAGAACTGT GGCTTGGACC TATGAGACTG ACCCAGGATC CAATCCAGGT GTTGCTAGTC 
TTTGCAAAAG AAGATAGCCA GAGCGATGGC TTCTGGTGGG CCTGTGATCG AGCGGGATAC 
AGATGTAATA TTGCTCGGAC GCCAGAGTCC GCAGTTGAGT GCTTTTTGGA TAAGCACCAT 
GAAATCATTG TCATAGATCA CAGGCATTCA AGATACCTCG ATGCAGAAGC TGTGTGCAGA 
TCAATACGAG CTACAAAACC CTCAGAGCAC ACAACAATTC TGGCTGTTGT TCCACGTGTA 
GCTTCTGATC AAGAGGAGAT GTCTGTGCTA CCACTTCTGA ATGTGGGATT TAATAGGAGA 
TTTGTGGAGA ACAGCAGTAT GATTGCATGT TACAATGAGC TGATCCAGAT AGAACATGGG 
GAGGTGCGAT CTCAGTTCAA ACTGCGGTCC TGTAACTCAA TATTTACAGC CTTAGACAAC 
TGCCAAGAAG CCATAGAAAT AACAAGTGAA GATCATATTA TACAGTATGT TAACCCAGCC 
TTTGAACGGA TGATGGGATA TCATAAGGAA GAACTTATTG GGAAGGAACT TACTGAACTG 
CCAAAAAGTG ACAAAAATCG GGCAGATCTG TTGGACACCA TTAACACGTG CATCAAAAAG 
GGAAAGGAAT GGCAAGGAAT CTATTTTGCA AGAAGGAAAT CAGGAGACAG CATTCAACAG 
CATGTAAAAG TGACCCCAGT AATTGGGCAA GGGGGGAAAA TTAGGCATTT TGTGTCTATC 
AAGAAGCTGT GCAATAGCAA TGACAACAAT AAACAGATCT ACAAGGTAAA TCGAGAAGAC 
AATGCACTCA CAGAGTCTCA CTCGTTTAGA CTTAAGAACA GAAGGAAAGA ATCAGTGGAT 
GTGAGATCTA TCACTTCTCG CAGTAGTGAT GCACCAAGTT TGCAGAACCA CAGATACTCT 
TCAATGGCTC GAATTCACAC AATGACTATT GAGGCTCCAA TAACAAAGGT CATAAACATC 
ATCAACGCAG CCCAGGAGAA CAGCCCCGTG ACTGTAGCCG AAGCTCTAGA CAGAGTTTTG 
GAAATTCTTA GAACAACTGA ACTCTATTCG CCTCAGCTGG GAACCAAAGA TGAAGACCCC 
CACACCAGTG ATCTTGTAGG AGGTTTGATG ACTGATGGCT TAAGAAGGCT TTCAGGAAAT 
GAGTATGTTT TCTCCAAAAA TGTGCACCAC AGCCATAGCC ACCTTTCAGT GCCAATAACC 
ATCAATGACG TCCCACCTTG CATCGCAGAA TTACTAGAGG ATGAAGTTTC ATGGGACTTT 
AACATATTTG AATTAGAAGC TGTTACAATT AAAAGGCCGC TGGTATACCT TGGTTTAAAG 
ATATTTACTT GTTTTGGAGC CTGTGAGTTT TTGAATTGCT CAGAAGTGAC ACTTCGCTCC 
TGGTTACAGT TAGTTGAAGC CAACTATCAC GCATCAAATG CCTACCACAA CTCTACCCAT 
GCTGCCGATG TACTACATGC CACCGCTTTT TTCCTCCAGC AGGAGAATGT TAAGGGAAGC 
CTTGACCAGT TAGACAAAGT GGCGGCACTA ATTGCTGCCA CGGTACATGA TGTGGATCAT 
CCGGGCCGGA CTAATTCCTT CCTCTGTAAT GCCAGCAGCG AATTAGCCGT ATTGTACAAT 
GATACAGCAG TGTTGGAAAG CCATCATGCA GCACTCGCAT TTCAGCTCAC AGCAAAGGAC 
AATAAATGCA ACATCTTCAA GAACATGGAC CGGACTCAGT ATCGGACTCT CCGGCAGGCA 
ATCATTGACA TGGTTTTAGC AACAGAAATG ACCAAGCATT TTGAGCATGT CAACAAGTTC 
GTCAACAGCA TCAACAAACC CATTGCAGCC GAAGATAGCA ATTCCAACAG TGAGGGGAGT 
GATTTTGAAT GTTCCACCAA TATAAGGAAT TCTCCAGAGA ACCGGATCCT CATCAAACGC 
ATGATGATCA AATGCGCTGA CGTAGCCAAC CCCTGCCGCC CCCTGAACCT GTGCATAGAG 
TGGGCTGGAA GGATCTCCGA GGAGTATTTT GCACAGACAG ATGAAGAGAA GAGGCAAGGC 
TTGCCAGTGG TGATGCCAGT CTTTGACCGA AACACCTGCA GCATACCCAA GTCTCAGATC 
TCATTTATAG ACTATTTTAT TACAGACATG TTTGATGCGT GGGATGCCTT CGCCCATTTG 
CCCAACCTCA TCCTCCATCT GACAGACAAT TACAAGTACT GGAAATCATT AGATGAATTG 
AAGTGTAAAA GTCTCAGGTG TTCGTCAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_004910389.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910446.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03337
Clone ID Related Accession (Same CDS sequence)	XM_004910389.3 , XM_004910389.4
Accession Version	XM_004910389.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGGT ATCATCTACA 
GAACTGTGGC TTGGACCTAT GAGACTGACC CAGGATCCAA TCCAGGTGTT GCTAGTCTTT 
GCAAAAGAAG ATAGCCAGAG CGATGGCTTC TGGTGGGCCT GTGATCGAGC GGGATACAGA 
TGTAATATTG CTCGGACGCC AGAGTCCGCA GTTGAGTGCT TTTTGGATAA GCACCATGAA 
ATCATTGTCA TAGATCACAG GCATTCAAGA TACCTCGATG CAGAAGCTGT GTGCAGATCA 
ATACGAGCTA CAAAACCCTC AGAGCACACA ACAATTCTGG CTGTTGTTCC ACGTGTAGCT 
TCTGATCAAG AGGAGATGTC TGTGCTACCA CTTCTGAATG TGGGATTTAA TAGGAGATTT 
GTGGAGAACA GCAGTATGAT TGCATGTTAC AATGAGCTGA TCCAGATAGA ACATGGGGAG 
GTGCGATCTC AGTTCAAACT GCGGTCCTGT AACTCAATAT TTACAGCCTT AGACAACTGC 
CAAGAAGCCA TAGAAATAAC AAGTGAAGAT CATATTATAC AGTATGTTAA CCCAGCCTTT 
GAACGGATGA TGGGATATCA TAAGGAAGAA CTTATTGGGA AGGAACTTAC TGAACTGCCA 
AAAAGTGACA AAAATCGGGC AGATCTGTTG GACACCATTA ACACGTGCAT CAAAAAGGGA 
AAGGAATGGC AAGGAATCTA TTTTGCAAGA AGGAAATCAG GAGACAGCAT TCAACAGCAT 
GTAAAAGTGA CCCCAGTAAT TGGGCAAGGG GGGAAAATTA GGCATTTTGT GTCTATCAAG 
AAGCTGTGCA ATAGCAATGA CAACAATAAA CAGATCTACA AGGTAAATCG AGAAGACAAT 
GCACTCACAG AGTCTCACTC GTTTAGACTT AAGAACAGAA GGAAAGAATC AGTGGATGTG 
AGATCTATCA CTTCTCGCAG TAGTGATGCA CCAAGTTTGC AGAACCACAG ATACTCTTCA 
ATGGCTCGAA TTCACACAAT GACTATTGAG GCTCCAATAA CAAAGGTCAT AAACATCATC 
AACGCAGCCC AGGAGAACAG CCCCGTGACT GTAGCCGAAG CTCTAGACAG AGTTTTGGAA 
ATTCTTAGAA CAACTGAACT CTATTCGCCT CAGCTGGGAA CCAAAGATGA AGACCCCCAC 
ACCAGTGATC TTGTAGGAGG TTTGATGACT GATGGCTTAA GAAGGCTTTC AGGAAATGAG 
TATGTTTTCT CCAAAAATGT GCACCACAGC CATAGCCACC TTTCAGTGCC AATAACCATC 
AATGACGTCC CACCTTGCAT CGCAGAATTA CTAGAGGATG AAGTTTCATG GGACTTTAAC 
ATATTTGAAT TAGAAGCTGT TACAATTAAA AGGCCGCTGG TATACCTTGG TTTAAAGATA 
TTTACTTGTT TTGGAGCCTG TGAGTTTTTG AATTGCTCAG AAGTGACACT TCGCTCCTGG 
TTACAGTTAG TTGAAGCCAA CTATCACGCA TCAAATGCCT ACCACAACTC TACCCATGCT 
GCCGATGTAC TACATGCCAC CGCTTTTTTC CTCCAGCAGG AGAATGTTAA GGGAAGCCTT 
GACCAGTTAG ACAAAGTGGC GGCACTAATT GCTGCCACGG TACATGATGT GGATCATCCG 
GGCCGGACTA ATTCCTTCCT CTGTAATGCC AGCAGCGAAT TAGCCGTATT GTACAATGAT 
ACAGCAGTGT TGGAAAGCCA TCATGCAGCA CTCGCATTTC AGCTCACAGC AAAGGACAAT 
AAATGCAACA TCTTCAAGAA CATGGACCGG ACTCAGTATC GGACTCTCCG GCAGGCAATC 
ATTGACATGG TTTTAGCAAC AGAAATGACC AAGCATTTTG AGCATGTCAA CAAGTTCGTC 
AACAGCATCA ACAAACCCAT TGCAGCCGAA GATAGCAATT CCAACAGTGA GGGGAGTGAT 
TTTGAATGTT CCACCAATAT AAGGAATTCT CCAGAGAACC GGATCCTCAT CAAACGCATG 
ATGATCAAAT GCGCTGACGT AGCCAACCCC TGCCGCCCCC TGAACCTGTG CATAGAGTGG 
GCTGGAAGGA TCTCCGAGGA GTATTTTGCA CAGACAGATG AAGAGAAGAG GCAAGGCTTG 
CCAGTGGTGA TGCCAGTCTT TGACCGAAAC ACCTGCAGCA TACCCAAGTC TCAGATCTCA 
TTTATAGACT ATTTTATTAC AGACATGTTT GATGCGTGGG ATGCCTTCGC CCATTTGCCC 
AACCTCATCC TCCATCTGAC AGACAATTAC AAGTACTGGA AATCATTAGA TGAATTGAAG 
TGTAAAAGTC TCAGGTGTTC GTCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910446.1
CDS	1145..3754
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTAATLHGLFIKTDAADSIPSVLAYQGRYPPAARSGSRKGRRDQGPHSCIEAETQTSHTSVKVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_004910389.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910389.3

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGGT ATCATCTACA 
GAACTGTGGC TTGGACCTAT GAGACTGACC CAGGATCCAA TCCAGGTGTT GCTAGTCTTT 
GCAAAAGAAG ATAGCCAGAG CGATGGCTTC TGGTGGGCCT GTGATCGAGC GGGATACAGA 
TGTAATATTG CTCGGACGCC AGAGTCCGCA GTTGAGTGCT TTTTGGATAA GCACCATGAA 
ATCATTGTCA TAGATCACAG GCATTCAAGA TACCTCGATG CAGAAGCTGT GTGCAGATCA 
ATACGAGCTA CAAAACCCTC AGAGCACACA ACAATTCTGG CTGTTGTTCC ACGTGTAGCT 
TCTGATCAAG AGGAGATGTC TGTGCTACCA CTTCTGAATG TGGGATTTAA TAGGAGATTT 
GTGGAGAACA GCAGTATGAT TGCATGTTAC AATGAGCTGA TCCAGATAGA ACATGGGGAG 
GTGCGATCTC AGTTCAAACT GCGGTCCTGT AACTCAATAT TTACAGCCTT AGACAACTGC 
CAAGAAGCCA TAGAAATAAC AAGTGAAGAT CATATTATAC AGTATGTTAA CCCAGCCTTT 
GAACGGATGA TGGGATATCA TAAGGAAGAA CTTATTGGGA AGGAACTTAC TGAACTGCCA 
AAAAGTGACA AAAATCGGGC AGATCTGTTG GACACCATTA ACACGTGCAT CAAAAAGGGA 
AAGGAATGGC AAGGAATCTA TTTTGCAAGA AGGAAATCAG GAGACAGCAT TCAACAGCAT 
GTAAAAGTGA CCCCAGTAAT TGGGCAAGGG GGGAAAATTA GGCATTTTGT GTCTATCAAG 
AAGCTGTGCA ATAGCAATGA CAACAATAAA CAGATCTACA AGGTAAATCG AGAAGACAAT 
GCACTCACAG AGTCTCACTC GTTTAGACTT AAGAACAGAA GGAAAGAATC AGTGGATGTG 
AGATCTATCA CTTCTCGCAG TAGTGATGCA CCAAGTTTGC AGAACCACAG ATACTCTTCA 
ATGGCTCGAA TTCACACAAT GACTATTGAG GCTCCAATAA CAAAGGTCAT AAACATCATC 
AACGCAGCCC AGGAGAACAG CCCCGTGACT GTAGCCGAAG CTCTAGACAG AGTTTTGGAA 
ATTCTTAGAA CAACTGAACT CTATTCGCCT CAGCTGGGAA CCAAAGATGA AGACCCCCAC 
ACCAGTGATC TTGTAGGAGG TTTGATGACT GATGGCTTAA GAAGGCTTTC AGGAAATGAG 
TATGTTTTCT CCAAAAATGT GCACCACAGC CATAGCCACC TTTCAGTGCC AATAACCATC 
AATGACGTCC CACCTTGCAT CGCAGAATTA CTAGAGGATG AAGTTTCATG GGACTTTAAC 
ATATTTGAAT TAGAAGCTGT TACAATTAAA AGGCCGCTGG TATACCTTGG TTTAAAGATA 
TTTACTTGTT TTGGAGCCTG TGAGTTTTTG AATTGCTCAG AAGTGACACT TCGCTCCTGG 
TTACAGTTAG TTGAAGCCAA CTATCACGCA TCAAATGCCT ACCACAACTC TACCCATGCT 
GCCGATGTAC TACATGCCAC CGCTTTTTTC CTCCAGCAGG AGAATGTTAA GGGAAGCCTT 
GACCAGTTAG ACAAAGTGGC GGCACTAATT GCTGCCACGG TACATGATGT GGATCATCCG 
GGCCGGACTA ATTCCTTCCT CTGTAATGCC AGCAGCGAAT TAGCCGTATT GTACAATGAT 
ACAGCAGTGT TGGAAAGCCA TCATGCAGCA CTCGCATTTC AGCTCACAGC AAAGGACAAT 
AAATGCAACA TCTTCAAGAA CATGGACCGG ACTCAGTATC GGACTCTCCG GCAGGCAATC 
ATTGACATGG TTTTAGCAAC AGAAATGACC AAGCATTTTG AGCATGTCAA CAAGTTCGTC 
AACAGCATCA ACAAACCCAT TGCAGCCGAA GATAGCAATT CCAACAGTGA GGGGAGTGAT 
TTTGAATGTT CCACCAATAT AAGGAATTCT CCAGAGAACC GGATCCTCAT CAAACGCATG 
ATGATCAAAT GCGCTGACGT AGCCAACCCC TGCCGCCCCC TGAACCTGTG CATAGAGTGG 
GCTGGAAGGA TCTCCGAGGA GTATTTTGCA CAGACAGATG AAGAGAAGAG GCAAGGCTTG 
CCAGTGGTGA TGCCAGTCTT TGACCGAAAC ACCTGCAGCA TACCCAAGTC TCAGATCTCA 
TTTATAGACT ATTTTATTAC AGACATGTTT GATGCGTGGG ATGCCTTCGC CCATTTGCCC 
AACCTCATCC TCCATCTGAC AGACAATTAC AAGTACTGGA AATCATTAGA TGAATTGAAG 
TGTAAAAGTC TCAGGTGTTC GTCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_004910390.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910447.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

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CloneID	OXa03341
Clone ID Related Accession (Same CDS sequence)	XM_004910390.3 , XM_004910390.4
Accession Version	XM_004910390.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGC AGGTATCATC TACAGAACTG 
TGGCTTGGAC CTATGAGACT GACCCAGGAT CCAATCCAGG TGTTGCTAGT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCCTGTGATC GAGCGGGATA CAGATGTAAT 
ATTGCTCGGA CGCCAGAGTC CGCAGTTGAG TGCTTTTTGG ATAAGCACCA TGAAATCATT 
GTCATAGATC ACAGGCATTC AAGATACCTC GATGCAGAAG CTGTGTGCAG ATCAATACGA 
GCTACAAAAC CCTCAGAGCA CACAACAATT CTGGCTGTTG TTCCACGTGT AGCTTCTGAT 
CAAGAGGAGA TGTCTGTGCT ACCACTTCTG AATGTGGGAT TTAATAGGAG ATTTGTGGAG 
AACAGCAGTA TGATTGCATG TTACAATGAG CTGATCCAGA TAGAACATGG GGAGGTGCGA 
TCTCAGTTCA AACTGCGGTC CTGTAACTCA ATATTTACAG CCTTAGACAA CTGCCAAGAA 
GCCATAGAAA TAACAAGTGA AGATCATATT ATACAGTATG TTAACCCAGC CTTTGAACGG 
ATGATGGGAT ATCATAAGGA AGAACTTATT GGGAAGGAAC TTACTGAACT GCCAAAAAGT 
GACAAAAATC GGGCAGATCT GTTGGACACC ATTAACACGT GCATCAAAAA GGGAAAGGAA 
TGGCAAGGAA TCTATTTTGC AAGAAGGAAA TCAGGAGACA GCATTCAACA GCATGTAAAA 
GTGACCCCAG TAATTGGGCA AGGGGGGAAA ATTAGGCATT TTGTGTCTAT CAAGAAGCTG 
TGCAATAGCA ATGACAACAA TAAACAGATC TACAAGGTAA ATCGAGAAGA CAATGCACTC 
ACAGAGTCTC ACTCGTTTAG ACTTAAGAAC AGAAGGAAAG AATCAGTGGA TGTGAGATCT 
ATCACTTCTC GCAGTAGTGA TGCACCAAGT TTGCAGAACC ACAGATACTC TTCAATGGCT 
CGAATTCACA CAATGACTAT TGAGGCTCCA ATAACAAAGG TCATAAACAT CATCAACGCA 
GCCCAGGAGA ACAGCCCCGT GACTGTAGCC GAAGCTCTAG ACAGAGTTTT GGAAATTCTT 
AGAACAACTG AACTCTATTC GCCTCAGCTG GGAACCAAAG ATGAAGACCC CCACACCAGT 
GATCTTGTAG GAGGTTTGAT GACTGATGGC TTAAGAAGGC TTTCAGGAAA TGAGTATGTT 
TTCTCCAAAA ATGTGCACCA CAGCCATAGC CACCTTTCAG TGCCAATAAC CATCAATGAC 
GTCCCACCTT GCATCGCAGA ATTACTAGAG GATGAAGTTT CATGGGACTT TAACATATTT 
GAATTAGAAG CTGTTACAAT TAAAAGGCCG CTGGTATACC TTGGTTTAAA GATATTTACT 
TGTTTTGGAG CCTGTGAGTT TTTGAATTGC TCAGAAGTGA CACTTCGCTC CTGGTTACAG 
TTAGTTGAAG CCAACTATCA CGCATCAAAT GCCTACCACA ACTCTACCCA TGCTGCCGAT 
GTACTACATG CCACCGCTTT TTTCCTCCAG CAGGAGAATG TTAAGGGAAG CCTTGACCAG 
TTAGACAAAG TGGCGGCACT AATTGCTGCC ACGGTACATG ATGTGGATCA TCCGGGCCGG 
ACTAATTCCT TCCTCTGTAA TGCCAGCAGC GAATTAGCCG TATTGTACAA TGATACAGCA 
GTGTTGGAAA GCCATCATGC AGCACTCGCA TTTCAGCTCA CAGCAAAGGA CAATAAATGC 
AACATCTTCA AGAACATGGA CCGGACTCAG TATCGGACTC TCCGGCAGGC AATCATTGAC 
ATGGTTTTAG CAACAGAAAT GACCAAGCAT TTTGAGCATG TCAACAAGTT CGTCAACAGC 
ATCAACAAAC CCATTGCAGC CGAAGATAGC AATTCCAACA GTGAGGGGAG TGATTTTGAA 
TGTTCCACCA ATATAAGGAA TTCTCCAGAG AACCGGATCC TCATCAAACG CATGATGATC 
AAATGCGCTG ACGTAGCCAA CCCCTGCCGC CCCCTGAACC TGTGCATAGA GTGGGCTGGA 
AGGATCTCCG AGGAGTATTT TGCACAGACA GATGAAGAGA AGAGGCAAGG CTTGCCAGTG 
GTGATGCCAG TCTTTGACCG AAACACCTGC AGCATACCCA AGTCTCAGAT CTCATTTATA 
GACTATTTTA TTACAGACAT GTTTGATGCG TGGGATGCCT TCGCCCATTT GCCCAACCTC 
ATCCTCCATC TGACAGACAA TTACAAGTAC TGGAAATCAT TAGATGAATT GAAGTGTAAA 
AGTCTCAGGT GTTCGTCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910447.1
CDS	192..2555
Translation	MSVGSGSSREQQKQVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_004910390.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910390.3

ORF Insert Sequence:

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGC AGGTATCATC TACAGAACTG 
TGGCTTGGAC CTATGAGACT GACCCAGGAT CCAATCCAGG TGTTGCTAGT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCCTGTGATC GAGCGGGATA CAGATGTAAT 
ATTGCTCGGA CGCCAGAGTC CGCAGTTGAG TGCTTTTTGG ATAAGCACCA TGAAATCATT 
GTCATAGATC ACAGGCATTC AAGATACCTC GATGCAGAAG CTGTGTGCAG ATCAATACGA 
GCTACAAAAC CCTCAGAGCA CACAACAATT CTGGCTGTTG TTCCACGTGT AGCTTCTGAT 
CAAGAGGAGA TGTCTGTGCT ACCACTTCTG AATGTGGGAT TTAATAGGAG ATTTGTGGAG 
AACAGCAGTA TGATTGCATG TTACAATGAG CTGATCCAGA TAGAACATGG GGAGGTGCGA 
TCTCAGTTCA AACTGCGGTC CTGTAACTCA ATATTTACAG CCTTAGACAA CTGCCAAGAA 
GCCATAGAAA TAACAAGTGA AGATCATATT ATACAGTATG TTAACCCAGC CTTTGAACGG 
ATGATGGGAT ATCATAAGGA AGAACTTATT GGGAAGGAAC TTACTGAACT GCCAAAAAGT 
GACAAAAATC GGGCAGATCT GTTGGACACC ATTAACACGT GCATCAAAAA GGGAAAGGAA 
TGGCAAGGAA TCTATTTTGC AAGAAGGAAA TCAGGAGACA GCATTCAACA GCATGTAAAA 
GTGACCCCAG TAATTGGGCA AGGGGGGAAA ATTAGGCATT TTGTGTCTAT CAAGAAGCTG 
TGCAATAGCA ATGACAACAA TAAACAGATC TACAAGGTAA ATCGAGAAGA CAATGCACTC 
ACAGAGTCTC ACTCGTTTAG ACTTAAGAAC AGAAGGAAAG AATCAGTGGA TGTGAGATCT 
ATCACTTCTC GCAGTAGTGA TGCACCAAGT TTGCAGAACC ACAGATACTC TTCAATGGCT 
CGAATTCACA CAATGACTAT TGAGGCTCCA ATAACAAAGG TCATAAACAT CATCAACGCA 
GCCCAGGAGA ACAGCCCCGT GACTGTAGCC GAAGCTCTAG ACAGAGTTTT GGAAATTCTT 
AGAACAACTG AACTCTATTC GCCTCAGCTG GGAACCAAAG ATGAAGACCC CCACACCAGT 
GATCTTGTAG GAGGTTTGAT GACTGATGGC TTAAGAAGGC TTTCAGGAAA TGAGTATGTT 
TTCTCCAAAA ATGTGCACCA CAGCCATAGC CACCTTTCAG TGCCAATAAC CATCAATGAC 
GTCCCACCTT GCATCGCAGA ATTACTAGAG GATGAAGTTT CATGGGACTT TAACATATTT 
GAATTAGAAG CTGTTACAAT TAAAAGGCCG CTGGTATACC TTGGTTTAAA GATATTTACT 
TGTTTTGGAG CCTGTGAGTT TTTGAATTGC TCAGAAGTGA CACTTCGCTC CTGGTTACAG 
TTAGTTGAAG CCAACTATCA CGCATCAAAT GCCTACCACA ACTCTACCCA TGCTGCCGAT 
GTACTACATG CCACCGCTTT TTTCCTCCAG CAGGAGAATG TTAAGGGAAG CCTTGACCAG 
TTAGACAAAG TGGCGGCACT AATTGCTGCC ACGGTACATG ATGTGGATCA TCCGGGCCGG 
ACTAATTCCT TCCTCTGTAA TGCCAGCAGC GAATTAGCCG TATTGTACAA TGATACAGCA 
GTGTTGGAAA GCCATCATGC AGCACTCGCA TTTCAGCTCA CAGCAAAGGA CAATAAATGC 
AACATCTTCA AGAACATGGA CCGGACTCAG TATCGGACTC TCCGGCAGGC AATCATTGAC 
ATGGTTTTAG CAACAGAAAT GACCAAGCAT TTTGAGCATG TCAACAAGTT CGTCAACAGC 
ATCAACAAAC CCATTGCAGC CGAAGATAGC AATTCCAACA GTGAGGGGAG TGATTTTGAA 
TGTTCCACCA ATATAAGGAA TTCTCCAGAG AACCGGATCC TCATCAAACG CATGATGATC 
AAATGCGCTG ACGTAGCCAA CCCCTGCCGC CCCCTGAACC TGTGCATAGA GTGGGCTGGA 
AGGATCTCCG AGGAGTATTT TGCACAGACA GATGAAGAGA AGAGGCAAGG CTTGCCAGTG 
GTGATGCCAG TCTTTGACCG AAACACCTGC AGCATACCCA AGTCTCAGAT CTCATTTATA 
GACTATTTTA TTACAGACAT GTTTGATGCG TGGGATGCCT TCGCCCATTT GCCCAACCTC 
ATCCTCCATC TGACAGACAA TTACAAGTAC TGGAAATCAT TAGATGAATT GAAGTGTAAA 
AGTCTCAGGT GTTCGTCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_012967377.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822831.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa34852
Clone ID Related Accession (Same CDS sequence)	XM_012967377.2 , XM_012967377.3
Accession Version	XM_012967377.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGG TATCATCTAC AGAACTGTGG 
CTTGGACCTA TGAGACTGAC CCAGGATCCA ATCCAGGTGT TGCTAGTCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCC TGTGATCGAG CGGGATACAG ATGTAATATT 
GCTCGGACGC CAGAGTCCGC AGTTGAGTGC TTTTTGGATA AGCACCATGA AATCATTGTC 
ATAGATCACA GGCATTCAAG ATACCTCGAT GCAGAAGCTG TGTGCAGATC AATACGAGCT 
ACAAAACCCT CAGAGCACAC AACAATTCTG GCTGTTGTTC CACGTGTAGC TTCTGATCAA 
GAGGAGATGT CTGTGCTACC ACTTCTGAAT GTGGGATTTA ATAGGAGATT TGTGGAGAAC 
AGCAGTATGA TTGCATGTTA CAATGAGCTG ATCCAGATAG AACATGGGGA GGTGCGATCT 
CAGTTCAAAC TGCGGTCCTG TAACTCAATA TTTACAGCCT TAGACAACTG CCAAGAAGCC 
ATAGAAATAA CAAGTGAAGA TCATATTATA CAGTATGTTA ACCCAGCCTT TGAACGGATG 
ATGGGATATC ATAAGGAAGA ACTTATTGGG AAGGAACTTA CTGAACTGCC AAAAAGTGAC 
AAAAATCGGG CAGATCTGTT GGACACCATT AACACGTGCA TCAAAAAGGG AAAGGAATGG 
CAAGGAATCT ATTTTGCAAG AAGGAAATCA GGAGACAGCA TTCAACAGCA TGTAAAAGTG 
ACCCCAGTAA TTGGGCAAGG GGGGAAAATT AGGCATTTTG TGTCTATCAA GAAGCTGTGC 
AATAGCAATG ACAACAATAA ACAGATCTAC AAGGTAAATC GAGAAGACAA TGCACTCACA 
GAGTCTCACT CGTTTAGACT TAAGAACAGA AGGAAAGAAT CAGTGGATGT GAGATCTATC 
ACTTCTCGCA GTAGTGATGC ACCAAGTTTG CAGAACCACA GATACTCTTC AATGGCTCGA 
ATTCACACAA TGACTATTGA GGCTCCAATA ACAAAGGTCA TAAACATCAT CAACGCAGCC 
CAGGAGAACA GCCCCGTGAC TGTAGCCGAA GCTCTAGACA GAGTTTTGGA AATTCTTAGA 
ACAACTGAAC TCTATTCGCC TCAGCTGGGA ACCAAAGATG AAGACCCCCA CACCAGTGAT 
CTTGTAGGAG GTTTGATGAC TGATGGCTTA AGAAGGCTTT CAGGAAATGA GTATGTTTTC 
TCCAAAAATG TGCACCACAG CCATAGCCAC CTTTCAGTGC CAATAACCAT CAATGACGTC 
CCACCTTGCA TCGCAGAATT ACTAGAGGAT GAAGTTTCAT GGGACTTTAA CATATTTGAA 
TTAGAAGCTG TTACAATTAA AAGGCCGCTG GTATACCTTG GTTTAAAGAT ATTTACTTGT 
TTTGGAGCCT GTGAGTTTTT GAATTGCTCA GAAGTGACAC TTCGCTCCTG GTTACAGTTA 
GTTGAAGCCA ACTATCACGC ATCAAATGCC TACCACAACT CTACCCATGC TGCCGATGTA 
CTACATGCCA CCGCTTTTTT CCTCCAGCAG GAGAATGTTA AGGGAAGCCT TGACCAGTTA 
GACAAAGTGG CGGCACTAAT TGCTGCCACG GTACATGATG TGGATCATCC GGGCCGGACT 
AATTCCTTCC TCTGTAATGC CAGCAGCGAA TTAGCCGTAT TGTACAATGA TACAGCAGTG 
TTGGAAAGCC ATCATGCAGC ACTCGCATTT CAGCTCACAG CAAAGGACAA TAAATGCAAC 
ATCTTCAAGA ACATGGACCG GACTCAGTAT CGGACTCTCC GGCAGGCAAT CATTGACATG 
GTTTTAGCAA CAGAAATGAC CAAGCATTTT GAGCATGTCA ACAAGTTCGT CAACAGCATC 
AACAAACCCA TTGCAGCCGA AGATAGCAAT TCCAACAGTG AGGGGAGTGA TTTTGAATGT 
TCCACCAATA TAAGGAATTC TCCAGAGAAC CGGATCCTCA TCAAACGCAT GATGATCAAA 
TGCGCTGACG TAGCCAACCC CTGCCGCCCC CTGAACCTGT GCATAGAGTG GGCTGGAAGG 
ATCTCCGAGG AGTATTTTGC ACAGACAGAT GAAGAGAAGA GGCAAGGCTT GCCAGTGGTG 
ATGCCAGTCT TTGACCGAAA CACCTGCAGC ATACCCAAGT CTCAGATCTC ATTTATAGAC 
TATTTTATTA CAGACATGTT TGATGCGTGG GATGCCTTCG CCCATTTGCC CAACCTCATC 
CTCCATCTGA CAGACAATTA CAAGTACTGG AAATCATTAG ATGAATTGAA GTGTAAAAGT 
CTCAGGTGTT CGTCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822831.1
CDS	101..2461
Translation	MSVGSGSSREQQKVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_012967377.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967377.2

ORF Insert Sequence:

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGG TATCATCTAC AGAACTGTGG 
CTTGGACCTA TGAGACTGAC CCAGGATCCA ATCCAGGTGT TGCTAGTCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCC TGTGATCGAG CGGGATACAG ATGTAATATT 
GCTCGGACGC CAGAGTCCGC AGTTGAGTGC TTTTTGGATA AGCACCATGA AATCATTGTC 
ATAGATCACA GGCATTCAAG ATACCTCGAT GCAGAAGCTG TGTGCAGATC AATACGAGCT 
ACAAAACCCT CAGAGCACAC AACAATTCTG GCTGTTGTTC CACGTGTAGC TTCTGATCAA 
GAGGAGATGT CTGTGCTACC ACTTCTGAAT GTGGGATTTA ATAGGAGATT TGTGGAGAAC 
AGCAGTATGA TTGCATGTTA CAATGAGCTG ATCCAGATAG AACATGGGGA GGTGCGATCT 
CAGTTCAAAC TGCGGTCCTG TAACTCAATA TTTACAGCCT TAGACAACTG CCAAGAAGCC 
ATAGAAATAA CAAGTGAAGA TCATATTATA CAGTATGTTA ACCCAGCCTT TGAACGGATG 
ATGGGATATC ATAAGGAAGA ACTTATTGGG AAGGAACTTA CTGAACTGCC AAAAAGTGAC 
AAAAATCGGG CAGATCTGTT GGACACCATT AACACGTGCA TCAAAAAGGG AAAGGAATGG 
CAAGGAATCT ATTTTGCAAG AAGGAAATCA GGAGACAGCA TTCAACAGCA TGTAAAAGTG 
ACCCCAGTAA TTGGGCAAGG GGGGAAAATT AGGCATTTTG TGTCTATCAA GAAGCTGTGC 
AATAGCAATG ACAACAATAA ACAGATCTAC AAGGTAAATC GAGAAGACAA TGCACTCACA 
GAGTCTCACT CGTTTAGACT TAAGAACAGA AGGAAAGAAT CAGTGGATGT GAGATCTATC 
ACTTCTCGCA GTAGTGATGC ACCAAGTTTG CAGAACCACA GATACTCTTC AATGGCTCGA 
ATTCACACAA TGACTATTGA GGCTCCAATA ACAAAGGTCA TAAACATCAT CAACGCAGCC 
CAGGAGAACA GCCCCGTGAC TGTAGCCGAA GCTCTAGACA GAGTTTTGGA AATTCTTAGA 
ACAACTGAAC TCTATTCGCC TCAGCTGGGA ACCAAAGATG AAGACCCCCA CACCAGTGAT 
CTTGTAGGAG GTTTGATGAC TGATGGCTTA AGAAGGCTTT CAGGAAATGA GTATGTTTTC 
TCCAAAAATG TGCACCACAG CCATAGCCAC CTTTCAGTGC CAATAACCAT CAATGACGTC 
CCACCTTGCA TCGCAGAATT ACTAGAGGAT GAAGTTTCAT GGGACTTTAA CATATTTGAA 
TTAGAAGCTG TTACAATTAA AAGGCCGCTG GTATACCTTG GTTTAAAGAT ATTTACTTGT 
TTTGGAGCCT GTGAGTTTTT GAATTGCTCA GAAGTGACAC TTCGCTCCTG GTTACAGTTA 
GTTGAAGCCA ACTATCACGC ATCAAATGCC TACCACAACT CTACCCATGC TGCCGATGTA 
CTACATGCCA CCGCTTTTTT CCTCCAGCAG GAGAATGTTA AGGGAAGCCT TGACCAGTTA 
GACAAAGTGG CGGCACTAAT TGCTGCCACG GTACATGATG TGGATCATCC GGGCCGGACT 
AATTCCTTCC TCTGTAATGC CAGCAGCGAA TTAGCCGTAT TGTACAATGA TACAGCAGTG 
TTGGAAAGCC ATCATGCAGC ACTCGCATTT CAGCTCACAG CAAAGGACAA TAAATGCAAC 
ATCTTCAAGA ACATGGACCG GACTCAGTAT CGGACTCTCC GGCAGGCAAT CATTGACATG 
GTTTTAGCAA CAGAAATGAC CAAGCATTTT GAGCATGTCA ACAAGTTCGT CAACAGCATC 
AACAAACCCA TTGCAGCCGA AGATAGCAAT TCCAACAGTG AGGGGAGTGA TTTTGAATGT 
TCCACCAATA TAAGGAATTC TCCAGAGAAC CGGATCCTCA TCAAACGCAT GATGATCAAA 
TGCGCTGACG TAGCCAACCC CTGCCGCCCC CTGAACCTGT GCATAGAGTG GGCTGGAAGG 
ATCTCCGAGG AGTATTTTGC ACAGACAGAT GAAGAGAAGA GGCAAGGCTT GCCAGTGGTG 
ATGCCAGTCT TTGACCGAAA CACCTGCAGC ATACCCAAGT CTCAGATCTC ATTTATAGAC 
TATTTTATTA CAGACATGTT TGATGCGTGG GATGCCTTCG CCCATTTGCC CAACCTCATC 
CTCCATCTGA CAGACAATTA CAAGTACTGG AAATCATTAG ATGAATTGAA GTGTAAAAGT 
CTCAGGTGTT CGTCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_002940874.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940920.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

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CloneID	OXa03336
Clone ID Related Accession (Same CDS sequence)	XM_002940874.5 , XM_002940874.4
Accession Version	XM_002940874.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940874.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGCA GGTATCATCT 
ACAGAACTGT GGCTTGGACC TATGAGACTG ACCCAGGATC CAATCCAGGT GTTGCTAGTC 
TTTGCAAAAG AAGATAGCCA GAGCGATGGC TTCTGGTGGG CCTGTGATCG AGCGGGATAC 
AGATGTAATA TTGCTCGGAC GCCAGAGTCC GCAGTTGAGT GCTTTTTGGA TAAGCACCAT 
GAAATCATTG TCATAGATCA CAGGCATTCA AGATACCTCG ATGCAGAAGC TGTGTGCAGA 
TCAATACGAG CTACAAAACC CTCAGAGCAC ACAACAATTC TGGCTGTTGT TCCACGTGTA 
GCTTCTGATC AAGAGGAGAT GTCTGTGCTA CCACTTCTGA ATGTGGGATT TAATAGGAGA 
TTTGTGGAGA ACAGCAGTAT GATTGCATGT TACAATGAGC TGATCCAGAT AGAACATGGG 
GAGGTGCGAT CTCAGTTCAA ACTGCGGTCC TGTAACTCAA TATTTACAGC CTTAGACAAC 
TGCCAAGAAG CCATAGAAAT AACAAGTGAA GATCATATTA TACAGTATGT TAACCCAGCC 
TTTGAACGGA TGATGGGATA TCATAAGGAA GAACTTATTG GGAAGGAACT TACTGAACTG 
CCAAAAAGTG ACAAAAATCG GGCAGATCTG TTGGACACCA TTAACACGTG CATCAAAAAG 
GGAAAGGAAT GGCAAGGAAT CTATTTTGCA AGAAGGAAAT CAGGAGACAG CATTCAACAG 
CATGTAAAAG TGACCCCAGT AATTGGGCAA GGGGGGAAAA TTAGGCATTT TGTGTCTATC 
AAGAAGCTGT GCAATAGCAA TGACAACAAT AAACAGATCT ACAAGGTAAA TCGAGAAGAC 
AATGCACTCA CAGAGTCTCA CTCGTTTAGA CTTAAGAACA GAAGGAAAGA ATCAGTGGAT 
GTGAGATCTA TCACTTCTCG CAGTAGTGAT GCACCAAGTT TGCAGAACCA CAGATACTCT 
TCAATGGCTC GAATTCACAC AATGACTATT GAGGCTCCAA TAACAAAGGT CATAAACATC 
ATCAACGCAG CCCAGGAGAA CAGCCCCGTG ACTGTAGCCG AAGCTCTAGA CAGAGTTTTG 
GAAATTCTTA GAACAACTGA ACTCTATTCG CCTCAGCTGG GAACCAAAGA TGAAGACCCC 
CACACCAGTG ATCTTGTAGG AGGTTTGATG ACTGATGGCT TAAGAAGGCT TTCAGGAAAT 
GAGTATGTTT TCTCCAAAAA TGTGCACCAC AGCCATAGCC ACCTTTCAGT GCCAATAACC 
ATCAATGACG TCCCACCTTG CATCGCAGAA TTACTAGAGG ATGAAGTTTC ATGGGACTTT 
AACATATTTG AATTAGAAGC TGTTACAATT AAAAGGCCGC TGGTATACCT TGGTTTAAAG 
ATATTTACTT GTTTTGGAGC CTGTGAGTTT TTGAATTGCT CAGAAGTGAC ACTTCGCTCC 
TGGTTACAGT TAGTTGAAGC CAACTATCAC GCATCAAATG CCTACCACAA CTCTACCCAT 
GCTGCCGATG TACTACATGC CACCGCTTTT TTCCTCCAGC AGGAGAATGT TAAGGGAAGC 
CTTGACCAGT TAGACAAAGT GGCGGCACTA ATTGCTGCCA CGGTACATGA TGTGGATCAT 
CCGGGCCGGA CTAATTCCTT CCTCTGTAAT GCCAGCAGCG AATTAGCCGT ATTGTACAAT 
GATACAGCAG TGTTGGAAAG CCATCATGCA GCACTCGCAT TTCAGCTCAC AGCAAAGGAC 
AATAAATGCA ACATCTTCAA GAACATGGAC CGGACTCAGT ATCGGACTCT CCGGCAGGCA 
ATCATTGACA TGGTTTTAGC AACAGAAATG ACCAAGCATT TTGAGCATGT CAACAAGTTC 
GTCAACAGCA TCAACAAACC CATTGCAGCC GAAGATAGCA ATTCCAACAG TGAGGGGAGT 
GATTTTGAAT GTTCCACCAA TATAAGGAAT TCTCCAGAGA ACCGGATCCT CATCAAACGC 
ATGATGATCA AATGCGCTGA CGTAGCCAAC CCCTGCCGCC CCCTGAACCT GTGCATAGAG 
TGGGCTGGAA GGATCTCCGA GGAGTATTTT GCACAGACAG ATGAAGAGAA GAGGCAAGGC 
TTGCCAGTGG TGATGCCAGT CTTTGACCGA AACACCTGCA GCATACCCAA GTCTCAGATC 
TCATTTATAG ACTATTTTAT TACAGACATG TTTGATGCGT GGGATGCCTT CGCCCATTTG 
CCCAACCTCA TCCTCCATCT GACAGACAAT TACAAGTACT GGAAATCATT AGATGAATTG 
AAGTGTAAAA GTCTCAGGTG TTCGTCAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940920.1
CDS	936..3548
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTAATLHGLFIKTDAADSIPSVLAYQGRYPPAARSGSRKGRRDQGPHSCIEAETQTSHTSVKQVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_002940874.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940874.5

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGCA GGTATCATCT 
ACAGAACTGT GGCTTGGACC TATGAGACTG ACCCAGGATC CAATCCAGGT GTTGCTAGTC 
TTTGCAAAAG AAGATAGCCA GAGCGATGGC TTCTGGTGGG CCTGTGATCG AGCGGGATAC 
AGATGTAATA TTGCTCGGAC GCCAGAGTCC GCAGTTGAGT GCTTTTTGGA TAAGCACCAT 
GAAATCATTG TCATAGATCA CAGGCATTCA AGATACCTCG ATGCAGAAGC TGTGTGCAGA 
TCAATACGAG CTACAAAACC CTCAGAGCAC ACAACAATTC TGGCTGTTGT TCCACGTGTA 
GCTTCTGATC AAGAGGAGAT GTCTGTGCTA CCACTTCTGA ATGTGGGATT TAATAGGAGA 
TTTGTGGAGA ACAGCAGTAT GATTGCATGT TACAATGAGC TGATCCAGAT AGAACATGGG 
GAGGTGCGAT CTCAGTTCAA ACTGCGGTCC TGTAACTCAA TATTTACAGC CTTAGACAAC 
TGCCAAGAAG CCATAGAAAT AACAAGTGAA GATCATATTA TACAGTATGT TAACCCAGCC 
TTTGAACGGA TGATGGGATA TCATAAGGAA GAACTTATTG GGAAGGAACT TACTGAACTG 
CCAAAAAGTG ACAAAAATCG GGCAGATCTG TTGGACACCA TTAACACGTG CATCAAAAAG 
GGAAAGGAAT GGCAAGGAAT CTATTTTGCA AGAAGGAAAT CAGGAGACAG CATTCAACAG 
CATGTAAAAG TGACCCCAGT AATTGGGCAA GGGGGGAAAA TTAGGCATTT TGTGTCTATC 
AAGAAGCTGT GCAATAGCAA TGACAACAAT AAACAGATCT ACAAGGTAAA TCGAGAAGAC 
AATGCACTCA CAGAGTCTCA CTCGTTTAGA CTTAAGAACA GAAGGAAAGA ATCAGTGGAT 
GTGAGATCTA TCACTTCTCG CAGTAGTGAT GCACCAAGTT TGCAGAACCA CAGATACTCT 
TCAATGGCTC GAATTCACAC AATGACTATT GAGGCTCCAA TAACAAAGGT CATAAACATC 
ATCAACGCAG CCCAGGAGAA CAGCCCCGTG ACTGTAGCCG AAGCTCTAGA CAGAGTTTTG 
GAAATTCTTA GAACAACTGA ACTCTATTCG CCTCAGCTGG GAACCAAAGA TGAAGACCCC 
CACACCAGTG ATCTTGTAGG AGGTTTGATG ACTGATGGCT TAAGAAGGCT TTCAGGAAAT 
GAGTATGTTT TCTCCAAAAA TGTGCACCAC AGCCATAGCC ACCTTTCAGT GCCAATAACC 
ATCAATGACG TCCCACCTTG CATCGCAGAA TTACTAGAGG ATGAAGTTTC ATGGGACTTT 
AACATATTTG AATTAGAAGC TGTTACAATT AAAAGGCCGC TGGTATACCT TGGTTTAAAG 
ATATTTACTT GTTTTGGAGC CTGTGAGTTT TTGAATTGCT CAGAAGTGAC ACTTCGCTCC 
TGGTTACAGT TAGTTGAAGC CAACTATCAC GCATCAAATG CCTACCACAA CTCTACCCAT 
GCTGCCGATG TACTACATGC CACCGCTTTT TTCCTCCAGC AGGAGAATGT TAAGGGAAGC 
CTTGACCAGT TAGACAAAGT GGCGGCACTA ATTGCTGCCA CGGTACATGA TGTGGATCAT 
CCGGGCCGGA CTAATTCCTT CCTCTGTAAT GCCAGCAGCG AATTAGCCGT ATTGTACAAT 
GATACAGCAG TGTTGGAAAG CCATCATGCA GCACTCGCAT TTCAGCTCAC AGCAAAGGAC 
AATAAATGCA ACATCTTCAA GAACATGGAC CGGACTCAGT ATCGGACTCT CCGGCAGGCA 
ATCATTGACA TGGTTTTAGC AACAGAAATG ACCAAGCATT TTGAGCATGT CAACAAGTTC 
GTCAACAGCA TCAACAAACC CATTGCAGCC GAAGATAGCA ATTCCAACAG TGAGGGGAGT 
GATTTTGAAT GTTCCACCAA TATAAGGAAT TCTCCAGAGA ACCGGATCCT CATCAAACGC 
ATGATGATCA AATGCGCTGA CGTAGCCAAC CCCTGCCGCC CCCTGAACCT GTGCATAGAG 
TGGGCTGGAA GGATCTCCGA GGAGTATTTT GCACAGACAG ATGAAGAGAA GAGGCAAGGC 
TTGCCAGTGG TGATGCCAGT CTTTGACCGA AACACCTGCA GCATACCCAA GTCTCAGATC 
TCATTTATAG ACTATTTTAT TACAGACATG TTTGATGCGT GGGATGCCTT CGCCCATTTG 
CCCAACCTCA TCCTCCATCT GACAGACAAT TACAAGTACT GGAAATCATT AGATGAATTG 
AAGTGTAAAA GTCTCAGGTG TTCGTCAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_004910389.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910446.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03337
Clone ID Related Accession (Same CDS sequence)	XM_004910389.3 , XM_004910389.4
Accession Version	XM_004910389.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910389.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGGT ATCATCTACA 
GAACTGTGGC TTGGACCTAT GAGACTGACC CAGGATCCAA TCCAGGTGTT GCTAGTCTTT 
GCAAAAGAAG ATAGCCAGAG CGATGGCTTC TGGTGGGCCT GTGATCGAGC GGGATACAGA 
TGTAATATTG CTCGGACGCC AGAGTCCGCA GTTGAGTGCT TTTTGGATAA GCACCATGAA 
ATCATTGTCA TAGATCACAG GCATTCAAGA TACCTCGATG CAGAAGCTGT GTGCAGATCA 
ATACGAGCTA CAAAACCCTC AGAGCACACA ACAATTCTGG CTGTTGTTCC ACGTGTAGCT 
TCTGATCAAG AGGAGATGTC TGTGCTACCA CTTCTGAATG TGGGATTTAA TAGGAGATTT 
GTGGAGAACA GCAGTATGAT TGCATGTTAC AATGAGCTGA TCCAGATAGA ACATGGGGAG 
GTGCGATCTC AGTTCAAACT GCGGTCCTGT AACTCAATAT TTACAGCCTT AGACAACTGC 
CAAGAAGCCA TAGAAATAAC AAGTGAAGAT CATATTATAC AGTATGTTAA CCCAGCCTTT 
GAACGGATGA TGGGATATCA TAAGGAAGAA CTTATTGGGA AGGAACTTAC TGAACTGCCA 
AAAAGTGACA AAAATCGGGC AGATCTGTTG GACACCATTA ACACGTGCAT CAAAAAGGGA 
AAGGAATGGC AAGGAATCTA TTTTGCAAGA AGGAAATCAG GAGACAGCAT TCAACAGCAT 
GTAAAAGTGA CCCCAGTAAT TGGGCAAGGG GGGAAAATTA GGCATTTTGT GTCTATCAAG 
AAGCTGTGCA ATAGCAATGA CAACAATAAA CAGATCTACA AGGTAAATCG AGAAGACAAT 
GCACTCACAG AGTCTCACTC GTTTAGACTT AAGAACAGAA GGAAAGAATC AGTGGATGTG 
AGATCTATCA CTTCTCGCAG TAGTGATGCA CCAAGTTTGC AGAACCACAG ATACTCTTCA 
ATGGCTCGAA TTCACACAAT GACTATTGAG GCTCCAATAA CAAAGGTCAT AAACATCATC 
AACGCAGCCC AGGAGAACAG CCCCGTGACT GTAGCCGAAG CTCTAGACAG AGTTTTGGAA 
ATTCTTAGAA CAACTGAACT CTATTCGCCT CAGCTGGGAA CCAAAGATGA AGACCCCCAC 
ACCAGTGATC TTGTAGGAGG TTTGATGACT GATGGCTTAA GAAGGCTTTC AGGAAATGAG 
TATGTTTTCT CCAAAAATGT GCACCACAGC CATAGCCACC TTTCAGTGCC AATAACCATC 
AATGACGTCC CACCTTGCAT CGCAGAATTA CTAGAGGATG AAGTTTCATG GGACTTTAAC 
ATATTTGAAT TAGAAGCTGT TACAATTAAA AGGCCGCTGG TATACCTTGG TTTAAAGATA 
TTTACTTGTT TTGGAGCCTG TGAGTTTTTG AATTGCTCAG AAGTGACACT TCGCTCCTGG 
TTACAGTTAG TTGAAGCCAA CTATCACGCA TCAAATGCCT ACCACAACTC TACCCATGCT 
GCCGATGTAC TACATGCCAC CGCTTTTTTC CTCCAGCAGG AGAATGTTAA GGGAAGCCTT 
GACCAGTTAG ACAAAGTGGC GGCACTAATT GCTGCCACGG TACATGATGT GGATCATCCG 
GGCCGGACTA ATTCCTTCCT CTGTAATGCC AGCAGCGAAT TAGCCGTATT GTACAATGAT 
ACAGCAGTGT TGGAAAGCCA TCATGCAGCA CTCGCATTTC AGCTCACAGC AAAGGACAAT 
AAATGCAACA TCTTCAAGAA CATGGACCGG ACTCAGTATC GGACTCTCCG GCAGGCAATC 
ATTGACATGG TTTTAGCAAC AGAAATGACC AAGCATTTTG AGCATGTCAA CAAGTTCGTC 
AACAGCATCA ACAAACCCAT TGCAGCCGAA GATAGCAATT CCAACAGTGA GGGGAGTGAT 
TTTGAATGTT CCACCAATAT AAGGAATTCT CCAGAGAACC GGATCCTCAT CAAACGCATG 
ATGATCAAAT GCGCTGACGT AGCCAACCCC TGCCGCCCCC TGAACCTGTG CATAGAGTGG 
GCTGGAAGGA TCTCCGAGGA GTATTTTGCA CAGACAGATG AAGAGAAGAG GCAAGGCTTG 
CCAGTGGTGA TGCCAGTCTT TGACCGAAAC ACCTGCAGCA TACCCAAGTC TCAGATCTCA 
TTTATAGACT ATTTTATTAC AGACATGTTT GATGCGTGGG ATGCCTTCGC CCATTTGCCC 
AACCTCATCC TCCATCTGAC AGACAATTAC AAGTACTGGA AATCATTAGA TGAATTGAAG 
TGTAAAAGTC TCAGGTGTTC GTCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910446.1
CDS	936..3545
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTAATLHGLFIKTDAADSIPSVLAYQGRYPPAARSGSRKGRRDQGPHSCIEAETQTSHTSVKVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_004910389.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910389.4

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT CCACGTATCT CAGAGCGGAG TGATTTACTG TCGGGACTCG 
GACGAGTCAA ACTCCCCACA TCAGACAACC ACCATCTCCC AAGGCACCGC TGCCACCCTG 
CACGGACTCT TCATAAAGAC CGACGCAGCG GACTCCATCC CTTCAGTGCT TGCCTACCAG 
GGCCGATACC CGCCTGCTGC ACGCAGTGGC TCCCGCAAGG GGCGCAGGGA CCAGGGACCA 
CACAGCTGCA TAGAGGCAGA GACTCAGACA AGCCACACCA GTGTCAAGGT ATCATCTACA 
GAACTGTGGC TTGGACCTAT GAGACTGACC CAGGATCCAA TCCAGGTGTT GCTAGTCTTT 
GCAAAAGAAG ATAGCCAGAG CGATGGCTTC TGGTGGGCCT GTGATCGAGC GGGATACAGA 
TGTAATATTG CTCGGACGCC AGAGTCCGCA GTTGAGTGCT TTTTGGATAA GCACCATGAA 
ATCATTGTCA TAGATCACAG GCATTCAAGA TACCTCGATG CAGAAGCTGT GTGCAGATCA 
ATACGAGCTA CAAAACCCTC AGAGCACACA ACAATTCTGG CTGTTGTTCC ACGTGTAGCT 
TCTGATCAAG AGGAGATGTC TGTGCTACCA CTTCTGAATG TGGGATTTAA TAGGAGATTT 
GTGGAGAACA GCAGTATGAT TGCATGTTAC AATGAGCTGA TCCAGATAGA ACATGGGGAG 
GTGCGATCTC AGTTCAAACT GCGGTCCTGT AACTCAATAT TTACAGCCTT AGACAACTGC 
CAAGAAGCCA TAGAAATAAC AAGTGAAGAT CATATTATAC AGTATGTTAA CCCAGCCTTT 
GAACGGATGA TGGGATATCA TAAGGAAGAA CTTATTGGGA AGGAACTTAC TGAACTGCCA 
AAAAGTGACA AAAATCGGGC AGATCTGTTG GACACCATTA ACACGTGCAT CAAAAAGGGA 
AAGGAATGGC AAGGAATCTA TTTTGCAAGA AGGAAATCAG GAGACAGCAT TCAACAGCAT 
GTAAAAGTGA CCCCAGTAAT TGGGCAAGGG GGGAAAATTA GGCATTTTGT GTCTATCAAG 
AAGCTGTGCA ATAGCAATGA CAACAATAAA CAGATCTACA AGGTAAATCG AGAAGACAAT 
GCACTCACAG AGTCTCACTC GTTTAGACTT AAGAACAGAA GGAAAGAATC AGTGGATGTG 
AGATCTATCA CTTCTCGCAG TAGTGATGCA CCAAGTTTGC AGAACCACAG ATACTCTTCA 
ATGGCTCGAA TTCACACAAT GACTATTGAG GCTCCAATAA CAAAGGTCAT AAACATCATC 
AACGCAGCCC AGGAGAACAG CCCCGTGACT GTAGCCGAAG CTCTAGACAG AGTTTTGGAA 
ATTCTTAGAA CAACTGAACT CTATTCGCCT CAGCTGGGAA CCAAAGATGA AGACCCCCAC 
ACCAGTGATC TTGTAGGAGG TTTGATGACT GATGGCTTAA GAAGGCTTTC AGGAAATGAG 
TATGTTTTCT CCAAAAATGT GCACCACAGC CATAGCCACC TTTCAGTGCC AATAACCATC 
AATGACGTCC CACCTTGCAT CGCAGAATTA CTAGAGGATG AAGTTTCATG GGACTTTAAC 
ATATTTGAAT TAGAAGCTGT TACAATTAAA AGGCCGCTGG TATACCTTGG TTTAAAGATA 
TTTACTTGTT TTGGAGCCTG TGAGTTTTTG AATTGCTCAG AAGTGACACT TCGCTCCTGG 
TTACAGTTAG TTGAAGCCAA CTATCACGCA TCAAATGCCT ACCACAACTC TACCCATGCT 
GCCGATGTAC TACATGCCAC CGCTTTTTTC CTCCAGCAGG AGAATGTTAA GGGAAGCCTT 
GACCAGTTAG ACAAAGTGGC GGCACTAATT GCTGCCACGG TACATGATGT GGATCATCCG 
GGCCGGACTA ATTCCTTCCT CTGTAATGCC AGCAGCGAAT TAGCCGTATT GTACAATGAT 
ACAGCAGTGT TGGAAAGCCA TCATGCAGCA CTCGCATTTC AGCTCACAGC AAAGGACAAT 
AAATGCAACA TCTTCAAGAA CATGGACCGG ACTCAGTATC GGACTCTCCG GCAGGCAATC 
ATTGACATGG TTTTAGCAAC AGAAATGACC AAGCATTTTG AGCATGTCAA CAAGTTCGTC 
AACAGCATCA ACAAACCCAT TGCAGCCGAA GATAGCAATT CCAACAGTGA GGGGAGTGAT 
TTTGAATGTT CCACCAATAT AAGGAATTCT CCAGAGAACC GGATCCTCAT CAAACGCATG 
ATGATCAAAT GCGCTGACGT AGCCAACCCC TGCCGCCCCC TGAACCTGTG CATAGAGTGG 
GCTGGAAGGA TCTCCGAGGA GTATTTTGCA CAGACAGATG AAGAGAAGAG GCAAGGCTTG 
CCAGTGGTGA TGCCAGTCTT TGACCGAAAC ACCTGCAGCA TACCCAAGTC TCAGATCTCA 
TTTATAGACT ATTTTATTAC AGACATGTTT GATGCGTGGG ATGCCTTCGC CCATTTGCCC 
AACCTCATCC TCCATCTGAC AGACAATTAC AAGTACTGGA AATCATTAGA TGAATTGAAG 
TGTAAAAGTC TCAGGTGTTC GTCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_004910390.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910447.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

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CloneID	OXa03341
Clone ID Related Accession (Same CDS sequence)	XM_004910390.3 , XM_004910390.4
Accession Version	XM_004910390.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910390.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGC AGGTATCATC TACAGAACTG 
TGGCTTGGAC CTATGAGACT GACCCAGGAT CCAATCCAGG TGTTGCTAGT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCCTGTGATC GAGCGGGATA CAGATGTAAT 
ATTGCTCGGA CGCCAGAGTC CGCAGTTGAG TGCTTTTTGG ATAAGCACCA TGAAATCATT 
GTCATAGATC ACAGGCATTC AAGATACCTC GATGCAGAAG CTGTGTGCAG ATCAATACGA 
GCTACAAAAC CCTCAGAGCA CACAACAATT CTGGCTGTTG TTCCACGTGT AGCTTCTGAT 
CAAGAGGAGA TGTCTGTGCT ACCACTTCTG AATGTGGGAT TTAATAGGAG ATTTGTGGAG 
AACAGCAGTA TGATTGCATG TTACAATGAG CTGATCCAGA TAGAACATGG GGAGGTGCGA 
TCTCAGTTCA AACTGCGGTC CTGTAACTCA ATATTTACAG CCTTAGACAA CTGCCAAGAA 
GCCATAGAAA TAACAAGTGA AGATCATATT ATACAGTATG TTAACCCAGC CTTTGAACGG 
ATGATGGGAT ATCATAAGGA AGAACTTATT GGGAAGGAAC TTACTGAACT GCCAAAAAGT 
GACAAAAATC GGGCAGATCT GTTGGACACC ATTAACACGT GCATCAAAAA GGGAAAGGAA 
TGGCAAGGAA TCTATTTTGC AAGAAGGAAA TCAGGAGACA GCATTCAACA GCATGTAAAA 
GTGACCCCAG TAATTGGGCA AGGGGGGAAA ATTAGGCATT TTGTGTCTAT CAAGAAGCTG 
TGCAATAGCA ATGACAACAA TAAACAGATC TACAAGGTAA ATCGAGAAGA CAATGCACTC 
ACAGAGTCTC ACTCGTTTAG ACTTAAGAAC AGAAGGAAAG AATCAGTGGA TGTGAGATCT 
ATCACTTCTC GCAGTAGTGA TGCACCAAGT TTGCAGAACC ACAGATACTC TTCAATGGCT 
CGAATTCACA CAATGACTAT TGAGGCTCCA ATAACAAAGG TCATAAACAT CATCAACGCA 
GCCCAGGAGA ACAGCCCCGT GACTGTAGCC GAAGCTCTAG ACAGAGTTTT GGAAATTCTT 
AGAACAACTG AACTCTATTC GCCTCAGCTG GGAACCAAAG ATGAAGACCC CCACACCAGT 
GATCTTGTAG GAGGTTTGAT GACTGATGGC TTAAGAAGGC TTTCAGGAAA TGAGTATGTT 
TTCTCCAAAA ATGTGCACCA CAGCCATAGC CACCTTTCAG TGCCAATAAC CATCAATGAC 
GTCCCACCTT GCATCGCAGA ATTACTAGAG GATGAAGTTT CATGGGACTT TAACATATTT 
GAATTAGAAG CTGTTACAAT TAAAAGGCCG CTGGTATACC TTGGTTTAAA GATATTTACT 
TGTTTTGGAG CCTGTGAGTT TTTGAATTGC TCAGAAGTGA CACTTCGCTC CTGGTTACAG 
TTAGTTGAAG CCAACTATCA CGCATCAAAT GCCTACCACA ACTCTACCCA TGCTGCCGAT 
GTACTACATG CCACCGCTTT TTTCCTCCAG CAGGAGAATG TTAAGGGAAG CCTTGACCAG 
TTAGACAAAG TGGCGGCACT AATTGCTGCC ACGGTACATG ATGTGGATCA TCCGGGCCGG 
ACTAATTCCT TCCTCTGTAA TGCCAGCAGC GAATTAGCCG TATTGTACAA TGATACAGCA 
GTGTTGGAAA GCCATCATGC AGCACTCGCA TTTCAGCTCA CAGCAAAGGA CAATAAATGC 
AACATCTTCA AGAACATGGA CCGGACTCAG TATCGGACTC TCCGGCAGGC AATCATTGAC 
ATGGTTTTAG CAACAGAAAT GACCAAGCAT TTTGAGCATG TCAACAAGTT CGTCAACAGC 
ATCAACAAAC CCATTGCAGC CGAAGATAGC AATTCCAACA GTGAGGGGAG TGATTTTGAA 
TGTTCCACCA ATATAAGGAA TTCTCCAGAG AACCGGATCC TCATCAAACG CATGATGATC 
AAATGCGCTG ACGTAGCCAA CCCCTGCCGC CCCCTGAACC TGTGCATAGA GTGGGCTGGA 
AGGATCTCCG AGGAGTATTT TGCACAGACA GATGAAGAGA AGAGGCAAGG CTTGCCAGTG 
GTGATGCCAG TCTTTGACCG AAACACCTGC AGCATACCCA AGTCTCAGAT CTCATTTATA 
GACTATTTTA TTACAGACAT GTTTGATGCG TGGGATGCCT TCGCCCATTT GCCCAACCTC 
ATCCTCCATC TGACAGACAA TTACAAGTAC TGGAAATCAT TAGATGAATT GAAGTGTAAA 
AGTCTCAGGT GTTCGTCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910447.1
CDS	192..2555
Translation	MSVGSGSSREQQKQVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_004910390.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910390.4

ORF Insert Sequence:

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGC AGGTATCATC TACAGAACTG 
TGGCTTGGAC CTATGAGACT GACCCAGGAT CCAATCCAGG TGTTGCTAGT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCCTGTGATC GAGCGGGATA CAGATGTAAT 
ATTGCTCGGA CGCCAGAGTC CGCAGTTGAG TGCTTTTTGG ATAAGCACCA TGAAATCATT 
GTCATAGATC ACAGGCATTC AAGATACCTC GATGCAGAAG CTGTGTGCAG ATCAATACGA 
GCTACAAAAC CCTCAGAGCA CACAACAATT CTGGCTGTTG TTCCACGTGT AGCTTCTGAT 
CAAGAGGAGA TGTCTGTGCT ACCACTTCTG AATGTGGGAT TTAATAGGAG ATTTGTGGAG 
AACAGCAGTA TGATTGCATG TTACAATGAG CTGATCCAGA TAGAACATGG GGAGGTGCGA 
TCTCAGTTCA AACTGCGGTC CTGTAACTCA ATATTTACAG CCTTAGACAA CTGCCAAGAA 
GCCATAGAAA TAACAAGTGA AGATCATATT ATACAGTATG TTAACCCAGC CTTTGAACGG 
ATGATGGGAT ATCATAAGGA AGAACTTATT GGGAAGGAAC TTACTGAACT GCCAAAAAGT 
GACAAAAATC GGGCAGATCT GTTGGACACC ATTAACACGT GCATCAAAAA GGGAAAGGAA 
TGGCAAGGAA TCTATTTTGC AAGAAGGAAA TCAGGAGACA GCATTCAACA GCATGTAAAA 
GTGACCCCAG TAATTGGGCA AGGGGGGAAA ATTAGGCATT TTGTGTCTAT CAAGAAGCTG 
TGCAATAGCA ATGACAACAA TAAACAGATC TACAAGGTAA ATCGAGAAGA CAATGCACTC 
ACAGAGTCTC ACTCGTTTAG ACTTAAGAAC AGAAGGAAAG AATCAGTGGA TGTGAGATCT 
ATCACTTCTC GCAGTAGTGA TGCACCAAGT TTGCAGAACC ACAGATACTC TTCAATGGCT 
CGAATTCACA CAATGACTAT TGAGGCTCCA ATAACAAAGG TCATAAACAT CATCAACGCA 
GCCCAGGAGA ACAGCCCCGT GACTGTAGCC GAAGCTCTAG ACAGAGTTTT GGAAATTCTT 
AGAACAACTG AACTCTATTC GCCTCAGCTG GGAACCAAAG ATGAAGACCC CCACACCAGT 
GATCTTGTAG GAGGTTTGAT GACTGATGGC TTAAGAAGGC TTTCAGGAAA TGAGTATGTT 
TTCTCCAAAA ATGTGCACCA CAGCCATAGC CACCTTTCAG TGCCAATAAC CATCAATGAC 
GTCCCACCTT GCATCGCAGA ATTACTAGAG GATGAAGTTT CATGGGACTT TAACATATTT 
GAATTAGAAG CTGTTACAAT TAAAAGGCCG CTGGTATACC TTGGTTTAAA GATATTTACT 
TGTTTTGGAG CCTGTGAGTT TTTGAATTGC TCAGAAGTGA CACTTCGCTC CTGGTTACAG 
TTAGTTGAAG CCAACTATCA CGCATCAAAT GCCTACCACA ACTCTACCCA TGCTGCCGAT 
GTACTACATG CCACCGCTTT TTTCCTCCAG CAGGAGAATG TTAAGGGAAG CCTTGACCAG 
TTAGACAAAG TGGCGGCACT AATTGCTGCC ACGGTACATG ATGTGGATCA TCCGGGCCGG 
ACTAATTCCT TCCTCTGTAA TGCCAGCAGC GAATTAGCCG TATTGTACAA TGATACAGCA 
GTGTTGGAAA GCCATCATGC AGCACTCGCA TTTCAGCTCA CAGCAAAGGA CAATAAATGC 
AACATCTTCA AGAACATGGA CCGGACTCAG TATCGGACTC TCCGGCAGGC AATCATTGAC 
ATGGTTTTAG CAACAGAAAT GACCAAGCAT TTTGAGCATG TCAACAAGTT CGTCAACAGC 
ATCAACAAAC CCATTGCAGC CGAAGATAGC AATTCCAACA GTGAGGGGAG TGATTTTGAA 
TGTTCCACCA ATATAAGGAA TTCTCCAGAG AACCGGATCC TCATCAAACG CATGATGATC 
AAATGCGCTG ACGTAGCCAA CCCCTGCCGC CCCCTGAACC TGTGCATAGA GTGGGCTGGA 
AGGATCTCCG AGGAGTATTT TGCACAGACA GATGAAGAGA AGAGGCAAGG CTTGCCAGTG 
GTGATGCCAG TCTTTGACCG AAACACCTGC AGCATACCCA AGTCTCAGAT CTCATTTATA 
GACTATTTTA TTACAGACAT GTTTGATGCG TGGGATGCCT TCGCCCATTT GCCCAACCTC 
ATCCTCCATC TGACAGACAA TTACAAGTAC TGGAAATCAT TAGATGAATT GAAGTGTAAA 
AGTCTCAGGT GTTCGTCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_012967377.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822831.1 Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.

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CloneID	OXa34852
Clone ID Related Accession (Same CDS sequence)	XM_012967377.2 , XM_012967377.3
Accession Version	XM_012967377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGG TATCATCTAC AGAACTGTGG 
CTTGGACCTA TGAGACTGAC CCAGGATCCA ATCCAGGTGT TGCTAGTCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCC TGTGATCGAG CGGGATACAG ATGTAATATT 
GCTCGGACGC CAGAGTCCGC AGTTGAGTGC TTTTTGGATA AGCACCATGA AATCATTGTC 
ATAGATCACA GGCATTCAAG ATACCTCGAT GCAGAAGCTG TGTGCAGATC AATACGAGCT 
ACAAAACCCT CAGAGCACAC AACAATTCTG GCTGTTGTTC CACGTGTAGC TTCTGATCAA 
GAGGAGATGT CTGTGCTACC ACTTCTGAAT GTGGGATTTA ATAGGAGATT TGTGGAGAAC 
AGCAGTATGA TTGCATGTTA CAATGAGCTG ATCCAGATAG AACATGGGGA GGTGCGATCT 
CAGTTCAAAC TGCGGTCCTG TAACTCAATA TTTACAGCCT TAGACAACTG CCAAGAAGCC 
ATAGAAATAA CAAGTGAAGA TCATATTATA CAGTATGTTA ACCCAGCCTT TGAACGGATG 
ATGGGATATC ATAAGGAAGA ACTTATTGGG AAGGAACTTA CTGAACTGCC AAAAAGTGAC 
AAAAATCGGG CAGATCTGTT GGACACCATT AACACGTGCA TCAAAAAGGG AAAGGAATGG 
CAAGGAATCT ATTTTGCAAG AAGGAAATCA GGAGACAGCA TTCAACAGCA TGTAAAAGTG 
ACCCCAGTAA TTGGGCAAGG GGGGAAAATT AGGCATTTTG TGTCTATCAA GAAGCTGTGC 
AATAGCAATG ACAACAATAA ACAGATCTAC AAGGTAAATC GAGAAGACAA TGCACTCACA 
GAGTCTCACT CGTTTAGACT TAAGAACAGA AGGAAAGAAT CAGTGGATGT GAGATCTATC 
ACTTCTCGCA GTAGTGATGC ACCAAGTTTG CAGAACCACA GATACTCTTC AATGGCTCGA 
ATTCACACAA TGACTATTGA GGCTCCAATA ACAAAGGTCA TAAACATCAT CAACGCAGCC 
CAGGAGAACA GCCCCGTGAC TGTAGCCGAA GCTCTAGACA GAGTTTTGGA AATTCTTAGA 
ACAACTGAAC TCTATTCGCC TCAGCTGGGA ACCAAAGATG AAGACCCCCA CACCAGTGAT 
CTTGTAGGAG GTTTGATGAC TGATGGCTTA AGAAGGCTTT CAGGAAATGA GTATGTTTTC 
TCCAAAAATG TGCACCACAG CCATAGCCAC CTTTCAGTGC CAATAACCAT CAATGACGTC 
CCACCTTGCA TCGCAGAATT ACTAGAGGAT GAAGTTTCAT GGGACTTTAA CATATTTGAA 
TTAGAAGCTG TTACAATTAA AAGGCCGCTG GTATACCTTG GTTTAAAGAT ATTTACTTGT 
TTTGGAGCCT GTGAGTTTTT GAATTGCTCA GAAGTGACAC TTCGCTCCTG GTTACAGTTA 
GTTGAAGCCA ACTATCACGC ATCAAATGCC TACCACAACT CTACCCATGC TGCCGATGTA 
CTACATGCCA CCGCTTTTTT CCTCCAGCAG GAGAATGTTA AGGGAAGCCT TGACCAGTTA 
GACAAAGTGG CGGCACTAAT TGCTGCCACG GTACATGATG TGGATCATCC GGGCCGGACT 
AATTCCTTCC TCTGTAATGC CAGCAGCGAA TTAGCCGTAT TGTACAATGA TACAGCAGTG 
TTGGAAAGCC ATCATGCAGC ACTCGCATTT CAGCTCACAG CAAAGGACAA TAAATGCAAC 
ATCTTCAAGA ACATGGACCG GACTCAGTAT CGGACTCTCC GGCAGGCAAT CATTGACATG 
GTTTTAGCAA CAGAAATGAC CAAGCATTTT GAGCATGTCA ACAAGTTCGT CAACAGCATC 
AACAAACCCA TTGCAGCCGA AGATAGCAAT TCCAACAGTG AGGGGAGTGA TTTTGAATGT 
TCCACCAATA TAAGGAATTC TCCAGAGAAC CGGATCCTCA TCAAACGCAT GATGATCAAA 
TGCGCTGACG TAGCCAACCC CTGCCGCCCC CTGAACCTGT GCATAGAGTG GGCTGGAAGG 
ATCTCCGAGG AGTATTTTGC ACAGACAGAT GAAGAGAAGA GGCAAGGCTT GCCAGTGGTG 
ATGCCAGTCT TTGACCGAAA CACCTGCAGC ATACCCAAGT CTCAGATCTC ATTTATAGAC 
TATTTTATTA CAGACATGTT TGATGCGTGG GATGCCTTCG CCCATTTGCC CAACCTCATC 
CTCCATCTGA CAGACAATTA CAAGTACTGG AAATCATTAG ATGAATTGAA GTGTAAAAGT 
CTCAGGTGTT CGTCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822831.1
CDS	106..2466
Translation	MSVGSGSSREQQKVSSTELWLGPMRLTQDPIQVLLVFAKEDSQSDGFWWACDRAGYRCNIARTPESAVECFLDKHHEIIVIDHRHSRYLDAEAVCRSIRATKPSEHTTILAVVPRVASDQEEMSVLPLLNVGFNRRFVENSSMIACYNELIQIEHGEVRSQFKLRSCNSIFTALDNCQEAIEITSEDHIIQYVNPAFERMMGYHKEELIGKELTELPKSDKNRADLLDTINTCIKKGKEWQGIYFARRKSGDSIQQHVKVTPVIGQGGKIRHFVSIKKLCNSNDNNKQIYKVNREDNALTESHSFRLKNRRKESVDVRSITSRSSDAPSLQNHRYSSMARIHTMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFSKNVHHSHSHLSVPITINDVPPCIAELLEDEVSWDFNIFELEAVTIKRPLVYLGLKIFTCFGACEFLNCSEVTLRSWLQLVEANYHASNAYHNSTHAADVLHATAFFLQQENVKGSLDQLDKVAALIAATVHDVDHPGRTNSFLCNASSELAVLYNDTAVLESHHAALAFQLTAKDNKCNIFKNMDRTQYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPIAAEDSNSNSEGSDFECSTNIRNSPENRILIKRMMIKCADVANPCRPLNLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPNLILHLTDNYKYWKSLDELKCKSLRCSSE

Target ORF information:

RefSeq Version	XM_012967377.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 8B (pde8b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967377.3

ORF Insert Sequence:

ATGAGCGTGG GAAGCGGCAG CAGCAGAGAA CAGCAAAAGG TATCATCTAC AGAACTGTGG 
CTTGGACCTA TGAGACTGAC CCAGGATCCA ATCCAGGTGT TGCTAGTCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCC TGTGATCGAG CGGGATACAG ATGTAATATT 
GCTCGGACGC CAGAGTCCGC AGTTGAGTGC TTTTTGGATA AGCACCATGA AATCATTGTC 
ATAGATCACA GGCATTCAAG ATACCTCGAT GCAGAAGCTG TGTGCAGATC AATACGAGCT 
ACAAAACCCT CAGAGCACAC AACAATTCTG GCTGTTGTTC CACGTGTAGC TTCTGATCAA 
GAGGAGATGT CTGTGCTACC ACTTCTGAAT GTGGGATTTA ATAGGAGATT TGTGGAGAAC 
AGCAGTATGA TTGCATGTTA CAATGAGCTG ATCCAGATAG AACATGGGGA GGTGCGATCT 
CAGTTCAAAC TGCGGTCCTG TAACTCAATA TTTACAGCCT TAGACAACTG CCAAGAAGCC 
ATAGAAATAA CAAGTGAAGA TCATATTATA CAGTATGTTA ACCCAGCCTT TGAACGGATG 
ATGGGATATC ATAAGGAAGA ACTTATTGGG AAGGAACTTA CTGAACTGCC AAAAAGTGAC 
AAAAATCGGG CAGATCTGTT GGACACCATT AACACGTGCA TCAAAAAGGG AAAGGAATGG 
CAAGGAATCT ATTTTGCAAG AAGGAAATCA GGAGACAGCA TTCAACAGCA TGTAAAAGTG 
ACCCCAGTAA TTGGGCAAGG GGGGAAAATT AGGCATTTTG TGTCTATCAA GAAGCTGTGC 
AATAGCAATG ACAACAATAA ACAGATCTAC AAGGTAAATC GAGAAGACAA TGCACTCACA 
GAGTCTCACT CGTTTAGACT TAAGAACAGA AGGAAAGAAT CAGTGGATGT GAGATCTATC 
ACTTCTCGCA GTAGTGATGC ACCAAGTTTG CAGAACCACA GATACTCTTC AATGGCTCGA 
ATTCACACAA TGACTATTGA GGCTCCAATA ACAAAGGTCA TAAACATCAT CAACGCAGCC 
CAGGAGAACA GCCCCGTGAC TGTAGCCGAA GCTCTAGACA GAGTTTTGGA AATTCTTAGA 
ACAACTGAAC TCTATTCGCC TCAGCTGGGA ACCAAAGATG AAGACCCCCA CACCAGTGAT 
CTTGTAGGAG GTTTGATGAC TGATGGCTTA AGAAGGCTTT CAGGAAATGA GTATGTTTTC 
TCCAAAAATG TGCACCACAG CCATAGCCAC CTTTCAGTGC CAATAACCAT CAATGACGTC 
CCACCTTGCA TCGCAGAATT ACTAGAGGAT GAAGTTTCAT GGGACTTTAA CATATTTGAA 
TTAGAAGCTG TTACAATTAA AAGGCCGCTG GTATACCTTG GTTTAAAGAT ATTTACTTGT 
TTTGGAGCCT GTGAGTTTTT GAATTGCTCA GAAGTGACAC TTCGCTCCTG GTTACAGTTA 
GTTGAAGCCA ACTATCACGC ATCAAATGCC TACCACAACT CTACCCATGC TGCCGATGTA 
CTACATGCCA CCGCTTTTTT CCTCCAGCAG GAGAATGTTA AGGGAAGCCT TGACCAGTTA 
GACAAAGTGG CGGCACTAAT TGCTGCCACG GTACATGATG TGGATCATCC GGGCCGGACT 
AATTCCTTCC TCTGTAATGC CAGCAGCGAA TTAGCCGTAT TGTACAATGA TACAGCAGTG 
TTGGAAAGCC ATCATGCAGC ACTCGCATTT CAGCTCACAG CAAAGGACAA TAAATGCAAC 
ATCTTCAAGA ACATGGACCG GACTCAGTAT CGGACTCTCC GGCAGGCAAT CATTGACATG 
GTTTTAGCAA CAGAAATGAC CAAGCATTTT GAGCATGTCA ACAAGTTCGT CAACAGCATC 
AACAAACCCA TTGCAGCCGA AGATAGCAAT TCCAACAGTG AGGGGAGTGA TTTTGAATGT 
TCCACCAATA TAAGGAATTC TCCAGAGAAC CGGATCCTCA TCAAACGCAT GATGATCAAA 
TGCGCTGACG TAGCCAACCC CTGCCGCCCC CTGAACCTGT GCATAGAGTG GGCTGGAAGG 
ATCTCCGAGG AGTATTTTGC ACAGACAGAT GAAGAGAAGA GGCAAGGCTT GCCAGTGGTG 
ATGCCAGTCT TTGACCGAAA CACCTGCAGC ATACCCAAGT CTCAGATCTC ATTTATAGAC 
TATTTTATTA CAGACATGTT TGATGCGTGG GATGCCTTCG CCCATTTGCC CAACCTCATC 
CTCCATCTGA CAGACAATTA CAAGTACTGG AAATCATTAG ATGAATTGAA GTGTAAAAGT 
CTCAGGTGTT CGTCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pde8b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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