map3k1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following map3k1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa34788 XM_012966894.3
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa49165 XM_031894157.1
Latest version!
Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OXa34788 XM_012966894.2 Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa34788
    Clone ID Related Accession (Same CDS sequence) XM_012966894.2 , XM_012966894.3
    Accession Version XM_012966894.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCCG CGGCGGGGAG TCGGAGCGTG TCGTCTGGAT TAACGGCAGC CGCGGGCGGC 
    GTAGCGGGCT CCGGTAGCCC GGAGACAGGG GGGCAAAGAC TTCCAGAAGG GGCTCCGGGG
    GAGGCAGGGG GCGAGCAGCG AGCCGACTGG AGGCGCAAGC AGCTGCGCAA AGTTCGGAGT
    GTGGAAGTGG ATAAGCTGGC GGGGAGAGAG CCGGAACCTC CGCACGTATT GGCTGCCGGA
    GTGAGGGAAC AGGAGCCTGC TGCGGGGAGC GAACCCGAGG AGCCTTCGGC GGCACACAGC
    TCATGCCGTG ACATGGAGAA TAAAGAAACA CTCAGGGGGC TACAGAAAAT GGATGACCGC
    CCAGAGGAGC GCATGATCAG GGAAAAACTC AAGGCAACAT GCATGCCAGC CTGGAAACAT
    GAATGGTTGG AGAGGAAAAG CAGGCGAGGT CCTGTGGTCG TTAAACCCAT TCCTGTAAAA
    GCCGAGGGGT CTGAAACTTT CAAGGCAGAC TCCGTTGAAG GGTCGCTGAA TGGCTTGTCC
    CATGTTCATA AAGGAAGGCG AAGCCCTTCT CCCAGCAGCT CTTCCAGCTC ATCCAAATCC
    ACAAAGCCTG AATCGCCAGG GTTGAAGAGG AGAGGCCTTT CTCCGGTCCC TAGTGGACGT
    GTTACACCAC CTCGGAGAGC TCCCTCTCCT GATGGCTTCT CACCTTTCAG TCCTGAGGAA
    ACCAGTCGAA GGGTTCACAA AGTTATGCGC GCAAGACTTT ATCTGCTACA GCAGATTGGA
    CCTAACTCCT TTCTCATTGG AGGGGACAGC CCAGACAATA AATACAGAGT TTTTATTGGA
    CCTCAGGCCT GTAGCTGTGG CAGGGGAACA TTCTGCATCC ATCTTCTCTT CGTCATGTTA
    AGAGTCTTCC AGTTAGAACC ATCTGACACC ATGTTGTGGA GAAAAGCACT GAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGACGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTGTCA CGCATGTCCA GTTCACATAC ATTGTCATCA
    TCTACCACCT CTACATCTAG TTCAGACACC AGCATGAAAG ATGAGGAGGA GCAGATGTGC
    CCGATCTGCT TGCTAGGGAT GCTGGACGAG GAGAGTCTGA CTGTATGTGA AGAAGGCTGC
    AGAAACAAGT TACACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGCAG AAGAAATAGG
    GAACCTCTTA TCTGTCCTCT TTGTCGAGCA AAGTGGAGGT CGCAGGATTT CAACAGCGTC
    GAATTGCCAG GGCCTGTGGA TTCTCCTCCC ATTCTTAGGG CAGTTCAACA GCAGTCTCTG
    CAACAGCTGC CTACGCCTGG TTCCCAAAGG AGGCCACAGG ACACAAGCCT GAATCTTACA
    CACTATGGGG TCCAAAATAT TCCATTTGCT TATAAGGACA TGGCTGAGCC ATGGATTCAG
    GTTTTCGGAA TGGAGCTGGT GAGCTGTTTG TTTTCTCGAA ACTGGAATAT CAGAGAAATG
    GCCCTGAGGC GCCTCTCCCA TGATGTCAGT GGGGCCTTGC TGTTGGCAAA TGGTGAAAGC
    ACTGGAAATT CATGCAACAG CAGTGGGACT ACAAGTGGCA CATCCCTGAA TGCTGCCAGT
    GGCTCATCTC AGTCTACTAT CTCAGGGGAT GTTGTAGTGG AGGCTTGCTG CAGTGTGCTC
    TCCATGGTGT GTGCTGACCC AGTCTACAAG GTGTACGTCG CAGCTTTAAA AACATTACGG
    GCCATGCTGG TGTACACTCC CTGCCATACA ATTACAGAAA GAAAGAAGCT GCAGCGACTT
    CTCAATCCAG TGGTGGAGAC TATTCTTGTA AAATGCGCAG ATGCCAACAG CCGCACAAGT
    CAGCTTTCAG TGTCAACCTT GTTGGAACTA TGCAAAGGAC AAGCTGGAGA GTTGGCAGTT
    GGAAGAGAAA TACTCAAATC TGGGTCCATC GGCATTGGTG GGGTGGATTA TGTCTTGCAT
    TGTATTCTAG CAACGCAAGC AGAGCCCAAT AACTGGCAGT CACTACTTGG CCGCCTTTGT
    CTGATCGACA GACTTTTATT GGAGTTTCCT GCTGAATTTT ATCCTCACAT CATCAGTGGA
    GACCTCACAC AGGCAGATGC AGTCGTTGAC AGATATAAAA GCCTGTTGTC CCTGCTGAAT
    TTTGCCTTGC AGTCTATTGA TAACTCTCAT TCCATGGTGG GAAAACTATC CCGGAGAGTA
    TTTTTAAGCG CAGCAAGAAT GGTTGCAAGA GTGCCCCAAG TGTTCGTCAA GCTGCTGGAT
    ATGTTAAGTG TAACTAGCTC TACACATTAC ATTCGTATGC GGCGCCGGCT AATGGCCATA
    GCTGAAGAGA TGGATATTGT TGAAGTAATC CAGCAGGACG TAGAAGATTC TCAAGCAATG
    GAAAACCGCA GAGAGGCATT TGTTCAGCCA TCTGCCCCAG CCAATACCCC ATCAACCTCA
    CAAAGCAATT CTCCGGATAA TACTGTACAG CTGTCAGGTA AAGTGGCAAA AGAAGCGTGT
    GACTCAAAGT CTGCCTCAAG TCCCGAAGAC TTACGCGAGG CACTGGAAGT AATGTCATTG
    GCGTACCCTT CCTCTTCCGC CACAGCCGAG CAACCAAAGC CTGCCATTCT AACAAAACGT
    AGACCACACA GTCAATGCTT GAATTCCCCG TCTTCCAGTC ATCCCCATTC CTTTTTCCCT
    ACTTTACCTT CACCTTCTTC TACTCCTCCT GTGCCAGCTG GTCCTGTCAC AGATGCTACA
    AAGCCAAGGT CTCAGGGTTT CACCCCATGT AAACTCTCTT CAGCTTCCCC TCAAACTCAG
    AGGAAATTTT CCCTTCAAAT ACAAAAGCAC TATTCAGGCA GTAAAGAGCC AGAAAAACTT
    TCTCCAGTTT TTACTCAAGC AAGACCTCTG CCATCTCCTC AAATACACAG GCCAAAGCCT
    TCACGACCTA CCCCAGGGGA CCCCGGGAAG CCTGGAGAAG CTCAGAAGGG CAGCTTGACT
    CTTGACCTGA ATGTTTCTCC TTACGATAAC TGTGGCAGTA ACGTCAGTGC TGTCATTCCA
    AGCGATGATA CGGTTTTCAC ACCTGTAGAG GAGAAGTGCA GACTGGATGC AAATACAGAG
    CTCAATTCCA GCATGGAGGA TCTGCTGGAA GCTTCGATGC CTGCATGTGA TAGTACTGTC
    ACCTTTAAGT CGGAAGTTGC TGTGCTTTCT TCTGAGCGTG CAGAGACTGA CAACACATAC
    AAAGATGATG TCAATCACAA TCAGAAATGC AAGGAGAAAA TGGAAGCTGA GGAGGAAGAG
    GCTCTGGCTA TTGTTTTGGC AATGTCGGCA TCTCAGGATG CACTGCCAGT CATACCCCAG
    CTGCAAGTTC AAAATGGGGA AGACATCATC ATTATTCAGC AGGATACACC AGAGACTCTG
    CCTGGACATA CCAAAGCAAG AAATCATTAC AGGGAGGAAA TGGAATGGCT CAAGGGCCAG
    CAGATTGGCT TAGGAGCGTT TTCTTCCTGC TATCAAGCTC AGGATGTTGG AACCGGAACA
    TTAATGGCTG TGAAACAGGT AACCTATGTG AGGAACACAT CTACTGAGCA AGGAGAAGTG
    GTTGAAGCCC TCCGAGAAGA GATTAGGATG ATGAGCCACC TGTCCCATCC TAATATTATA
    CGCATGTTGG GTGCCACTTG TGAGAAGAGC AACTACAATC TGTTTATTGA GTGGATGGCA
    GGTGGCTCTG TTGCCCTTTT GCTCAGCAAA TATGGAGCAT TCAAGGAATT GGTTATCATA
    AACTATGCTG AACAACTGCT ACGTGGTCTA TCATACCTGC ATGAGAATCA GATTATACAC
    AGAGATATTA AAGGTGCCAA TTTGTTGATT GACAGCACTG GGCAGCGGCT GCGAATTGCT
    GATTTTGGCG CTGCAGCAAG GTTGGCATCA AAGGGAACTG GTGCGGGCGA GTTTCAAGGG
    CAGCTGCTTG GAACAATTGC CTTCATGGCA CCTGAGGTGC TGAGAGGGCA ACAGTATGGA
    AGAAGCTGTG ATGTGTGGAG TCTTGGCTGC AGCATAATAG AGATGGCATG TGCTAAACCT
    CCATGGAATG CAGAGAAGCA CTCTAACCAT CTTGCACTTA TATTCAAGAT TGCAAGTGCT
    ACAACGGCCC CAACGATCCC ACCACACCTC TCTCCAGGAT TACGGGATGT AACCCTCCGA
    TGTCTGGAGC TCCAGCCCCA AGATAGACCA CCATCCCGAG AACTACTGAA GCACCCGGTC
    TTTCGAACAA AGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822348.1
    CDS280..4617
    Translation

    Target ORF information:

    RefSeq Version XM_012966894.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966894.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCCG CGGCGGGGAG TCGGAGCGTG TCGTCTGGAT TAACGGCAGC CGCGGGCGGC 
    GTAGCGGGCT CCGGTAGCCC GGAGACAGGG GGGCAAAGAC TTCCAGAAGG GGCTCCGGGG
    GAGGCAGGGG GCGAGCAGCG AGCCGACTGG AGGCGCAAGC AGCTGCGCAA AGTTCGGAGT
    GTGGAAGTGG ATAAGCTGGC GGGGAGAGAG CCGGAACCTC CGCACGTATT GGCTGCCGGA
    GTGAGGGAAC AGGAGCCTGC TGCGGGGAGC GAACCCGAGG AGCCTTCGGC GGCACACAGC
    TCATGCCGTG ACATGGAGAA TAAAGAAACA CTCAGGGGGC TACAGAAAAT GGATGACCGC
    CCAGAGGAGC GCATGATCAG GGAAAAACTC AAGGCAACAT GCATGCCAGC CTGGAAACAT
    GAATGGTTGG AGAGGAAAAG CAGGCGAGGT CCTGTGGTCG TTAAACCCAT TCCTGTAAAA
    GCCGAGGGGT CTGAAACTTT CAAGGCAGAC TCCGTTGAAG GGTCGCTGAA TGGCTTGTCC
    CATGTTCATA AAGGAAGGCG AAGCCCTTCT CCCAGCAGCT CTTCCAGCTC ATCCAAATCC
    ACAAAGCCTG AATCGCCAGG GTTGAAGAGG AGAGGCCTTT CTCCGGTCCC TAGTGGACGT
    GTTACACCAC CTCGGAGAGC TCCCTCTCCT GATGGCTTCT CACCTTTCAG TCCTGAGGAA
    ACCAGTCGAA GGGTTCACAA AGTTATGCGC GCAAGACTTT ATCTGCTACA GCAGATTGGA
    CCTAACTCCT TTCTCATTGG AGGGGACAGC CCAGACAATA AATACAGAGT TTTTATTGGA
    CCTCAGGCCT GTAGCTGTGG CAGGGGAACA TTCTGCATCC ATCTTCTCTT CGTCATGTTA
    AGAGTCTTCC AGTTAGAACC ATCTGACACC ATGTTGTGGA GAAAAGCACT GAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGACGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTGTCA CGCATGTCCA GTTCACATAC ATTGTCATCA
    TCTACCACCT CTACATCTAG TTCAGACACC AGCATGAAAG ATGAGGAGGA GCAGATGTGC
    CCGATCTGCT TGCTAGGGAT GCTGGACGAG GAGAGTCTGA CTGTATGTGA AGAAGGCTGC
    AGAAACAAGT TACACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGCAG AAGAAATAGG
    GAACCTCTTA TCTGTCCTCT TTGTCGAGCA AAGTGGAGGT CGCAGGATTT CAACAGCGTC
    GAATTGCCAG GGCCTGTGGA TTCTCCTCCC ATTCTTAGGG CAGTTCAACA GCAGTCTCTG
    CAACAGCTGC CTACGCCTGG TTCCCAAAGG AGGCCACAGG ACACAAGCCT GAATCTTACA
    CACTATGGGG TCCAAAATAT TCCATTTGCT TATAAGGACA TGGCTGAGCC ATGGATTCAG
    GTTTTCGGAA TGGAGCTGGT GAGCTGTTTG TTTTCTCGAA ACTGGAATAT CAGAGAAATG
    GCCCTGAGGC GCCTCTCCCA TGATGTCAGT GGGGCCTTGC TGTTGGCAAA TGGTGAAAGC
    ACTGGAAATT CATGCAACAG CAGTGGGACT ACAAGTGGCA CATCCCTGAA TGCTGCCAGT
    GGCTCATCTC AGTCTACTAT CTCAGGGGAT GTTGTAGTGG AGGCTTGCTG CAGTGTGCTC
    TCCATGGTGT GTGCTGACCC AGTCTACAAG GTGTACGTCG CAGCTTTAAA AACATTACGG
    GCCATGCTGG TGTACACTCC CTGCCATACA ATTACAGAAA GAAAGAAGCT GCAGCGACTT
    CTCAATCCAG TGGTGGAGAC TATTCTTGTA AAATGCGCAG ATGCCAACAG CCGCACAAGT
    CAGCTTTCAG TGTCAACCTT GTTGGAACTA TGCAAAGGAC AAGCTGGAGA GTTGGCAGTT
    GGAAGAGAAA TACTCAAATC TGGGTCCATC GGCATTGGTG GGGTGGATTA TGTCTTGCAT
    TGTATTCTAG CAACGCAAGC AGAGCCCAAT AACTGGCAGT CACTACTTGG CCGCCTTTGT
    CTGATCGACA GACTTTTATT GGAGTTTCCT GCTGAATTTT ATCCTCACAT CATCAGTGGA
    GACCTCACAC AGGCAGATGC AGTCGTTGAC AGATATAAAA GCCTGTTGTC CCTGCTGAAT
    TTTGCCTTGC AGTCTATTGA TAACTCTCAT TCCATGGTGG GAAAACTATC CCGGAGAGTA
    TTTTTAAGCG CAGCAAGAAT GGTTGCAAGA GTGCCCCAAG TGTTCGTCAA GCTGCTGGAT
    ATGTTAAGTG TAACTAGCTC TACACATTAC ATTCGTATGC GGCGCCGGCT AATGGCCATA
    GCTGAAGAGA TGGATATTGT TGAAGTAATC CAGCAGGACG TAGAAGATTC TCAAGCAATG
    GAAAACCGCA GAGAGGCATT TGTTCAGCCA TCTGCCCCAG CCAATACCCC ATCAACCTCA
    CAAAGCAATT CTCCGGATAA TACTGTACAG CTGTCAGGTA AAGTGGCAAA AGAAGCGTGT
    GACTCAAAGT CTGCCTCAAG TCCCGAAGAC TTACGCGAGG CACTGGAAGT AATGTCATTG
    GCGTACCCTT CCTCTTCCGC CACAGCCGAG CAACCAAAGC CTGCCATTCT AACAAAACGT
    AGACCACACA GTCAATGCTT GAATTCCCCG TCTTCCAGTC ATCCCCATTC CTTTTTCCCT
    ACTTTACCTT CACCTTCTTC TACTCCTCCT GTGCCAGCTG GTCCTGTCAC AGATGCTACA
    AAGCCAAGGT CTCAGGGTTT CACCCCATGT AAACTCTCTT CAGCTTCCCC TCAAACTCAG
    AGGAAATTTT CCCTTCAAAT ACAAAAGCAC TATTCAGGCA GTAAAGAGCC AGAAAAACTT
    TCTCCAGTTT TTACTCAAGC AAGACCTCTG CCATCTCCTC AAATACACAG GCCAAAGCCT
    TCACGACCTA CCCCAGGGGA CCCCGGGAAG CCTGGAGAAG CTCAGAAGGG CAGCTTGACT
    CTTGACCTGA ATGTTTCTCC TTACGATAAC TGTGGCAGTA ACGTCAGTGC TGTCATTCCA
    AGCGATGATA CGGTTTTCAC ACCTGTAGAG GAGAAGTGCA GACTGGATGC AAATACAGAG
    CTCAATTCCA GCATGGAGGA TCTGCTGGAA GCTTCGATGC CTGCATGTGA TAGTACTGTC
    ACCTTTAAGT CGGAAGTTGC TGTGCTTTCT TCTGAGCGTG CAGAGACTGA CAACACATAC
    AAAGATGATG TCAATCACAA TCAGAAATGC AAGGAGAAAA TGGAAGCTGA GGAGGAAGAG
    GCTCTGGCTA TTGTTTTGGC AATGTCGGCA TCTCAGGATG CACTGCCAGT CATACCCCAG
    CTGCAAGTTC AAAATGGGGA AGACATCATC ATTATTCAGC AGGATACACC AGAGACTCTG
    CCTGGACATA CCAAAGCAAG AAATCATTAC AGGGAGGAAA TGGAATGGCT CAAGGGCCAG
    CAGATTGGCT TAGGAGCGTT TTCTTCCTGC TATCAAGCTC AGGATGTTGG AACCGGAACA
    TTAATGGCTG TGAAACAGGT AACCTATGTG AGGAACACAT CTACTGAGCA AGGAGAAGTG
    GTTGAAGCCC TCCGAGAAGA GATTAGGATG ATGAGCCACC TGTCCCATCC TAATATTATA
    CGCATGTTGG GTGCCACTTG TGAGAAGAGC AACTACAATC TGTTTATTGA GTGGATGGCA
    GGTGGCTCTG TTGCCCTTTT GCTCAGCAAA TATGGAGCAT TCAAGGAATT GGTTATCATA
    AACTATGCTG AACAACTGCT ACGTGGTCTA TCATACCTGC ATGAGAATCA GATTATACAC
    AGAGATATTA AAGGTGCCAA TTTGTTGATT GACAGCACTG GGCAGCGGCT GCGAATTGCT
    GATTTTGGCG CTGCAGCAAG GTTGGCATCA AAGGGAACTG GTGCGGGCGA GTTTCAAGGG
    CAGCTGCTTG GAACAATTGC CTTCATGGCA CCTGAGGTGC TGAGAGGGCA ACAGTATGGA
    AGAAGCTGTG ATGTGTGGAG TCTTGGCTGC AGCATAATAG AGATGGCATG TGCTAAACCT
    CCATGGAATG CAGAGAAGCA CTCTAACCAT CTTGCACTTA TATTCAAGAT TGCAAGTGCT
    ACAACGGCCC CAACGATCCC ACCACACCTC TCTCCAGGAT TACGGGATGT AACCCTCCGA
    TGTCTGGAGC TCCAGCCCCA AGATAGACCA CCATCCCGAG AACTACTGAA GCACCCGGTC
    TTTCGAACAA AGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49165
    Clone ID Related Accession (Same CDS sequence) XM_031894157.1
    Accession Version XM_031894157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAATCA ATCTTTGTAG CCGTGACATG GAGAATAAAG AAACACTCAG GGGGCTACAG 
    AAAATGGATG ACCGCCCAGA GGAGCGCATG ATCAGGGAAA AACTCAAGGC AACATGCATG
    CCAGCCTGGA AACATGAATG GTTGGAGAGG AAAAGCAGGC GAGGTCCTGT GGTCGTTAAA
    CCCATTCCTG TAAAAGCCGA GGGGTCTGAA ACTTTCAAGG CAGACTCCGT TGAAGGGTCG
    CTGAATGGCT TGTCCCATGT TCATAAAGGA AGGCGAAGCC CTTCTCCCAG CAGCTCTTCC
    AGCTCATCCA AATCCACAAA GCCTGAATCG CCAGGGTTGA AGAGGAGAGG CCTTTCTCCG
    GTCCCTAGTG GACGTGTTAC ACCACCTCGG AGAGCTCCCT CTCCTGATGG CTTCTCACCT
    TTCAGTCCTG AGGAAACCAG TCGAAGGGTT CACAAAGTTA TGCGCGCAAG ACTTTATCTG
    CTACAGCAGA TTGGACCTAA CTCCTTTCTC ATTGGAGGGG ACAGCCCAGA CAATAAATAC
    AGAGTTTTTA TTGGACCTCA GGCCTGTAGC TGTGGCAGGG GAACATTCTG CATCCATCTT
    CTCTTCGTCA TGTTAAGAGT CTTCCAGTTA GAACCATCTG ACACCATGTT GTGGAGAAAA
    GCACTGAAGA ATTTTGAGGT TGAGAGTTTG TTCCAGAAAT ATCACAGTAG ACGTAGCTCA
    AGGATCAAAG CTCCATCTCG TAACACCATC CAGAAGTTTG TGTCACGCAT GTCCAGTTCA
    CATACATTGT CATCATCTAC CACCTCTACA TCTAGTTCAG ACACCAGCAT GAAAGATGAG
    GAGGAGCAGA TGTGCCCGAT CTGCTTGCTA GGGATGCTGG ACGAGGAGAG TCTGACTGTA
    TGTGAAGAAG GCTGCAGAAA CAAGTTACAC CACCACTGCA TGTCAATTTG GGCAGAAGAG
    TGCAGAAGAA ATAGGGAACC TCTTATCTGT CCTCTTTGTC GAGCAAAGTG GAGGTCGCAG
    GATTTCAACA GCGTCGAATT GCCAGGGCCT GTGGATTCTC CTCCCATTCT TAGGGCAGTT
    CAACAGCAGT CTCTGCAACA GCTGCCTACG CCTGGTTCCC AAAGGAGGCC ACAGGACACA
    AGCCTGAATC TTACACACTA TGGGGTCCAA AATATTCCAT TTGCTTATAA GGACATGGCT
    GAGCCATGGA TTCAGGTTTT CGGAATGGAG CTGGTGAGCT GTTTGTTTTC TCGAAACTGG
    AATATCAGAG AAATGGCCCT GAGGCGCCTC TCCCATGATG TCAGTGGGGC CTTGCTGTTG
    GCAAATGGTG AAAGCACTGG AAATTCATGC AACAGCAGTG GGACTACAAG TGGCACATCC
    CTGAATGCTG CCAGTGGCTC ATCTCAGTCT ACTATCTCAG GGGATGTTGT AGTGGAGGCT
    TGCTGCAGTG TGCTCTCCAT GGTGTGTGCT GACCCAGTCT ACAAGGTGTA CGTCGCAGCT
    TTAAAAACAT TACGGGCCAT GCTGGTGTAC ACTCCCTGCC ATACAATTAC AGAAAGAAAG
    AAGCTGCAGC GACTTCTCAA TCCAGTGGTG GAGACTATTC TTGTAAAATG CGCAGATGCC
    AACAGCCGCA CAAGTCAGCT TTCAGTGTCA ACCTTGTTGG AACTATGCAA AGGACAAGCT
    GGAGAGTTGG CAGTTGGAAG AGAAATACTC AAATCTGGGT CCATCGGCAT TGGTGGGGTG
    GATTATGTCT TGCATTGTAT TCTAGCAACG CAAGCAGAGC CCAATAACTG GCAGTCACTA
    CTTGGCCGCC TTTGTCTGAT CGACAGACTT TTATTGGAGT TTCCTGCTGA ATTTTATCCT
    CACATCATCA GTGGAGACCT CACACAGGCA GATGCAGTCG TTGACAGATA TAAAAGCCTG
    TTGTCCCTGC TGAATTTTGC CTTGCAGTCT ATTGATAACT CTCATTCCAT GGTGGGAAAA
    CTATCCCGGA GAGTATTTTT AAGCGCAGCA AGAATGGTTG CAAGAGTGCC CCAAGTGTTC
    GTCAAGCTGC TGGATATGTT AAGTGTAACT AGCTCTACAC ATTACATTCG TATGCGGCGC
    CGGCTAATGG CCATAGCTGA AGAGATGGAT ATTGTTGAAG TAATCCAGCA GGACGTAGAA
    GATTCTCAAG CAATGGAAAA CCGCAGAGAG GCATTTGTTC AGCCATCTGC CCCAGCCAAT
    ACCCCATCAA CCTCACAAAG CAATTCTCCG GATAATACTG TACAGCTGTC AGGTAAAGTG
    GCAAAAGAAG CGTGTGACTC AAAGTCTGCC TCAAGTCCCG AAGACTTACG CGAGGCACTG
    GAAGTAATGT CATTGGCGTA CCCTTCCTCT TCCGCCACAG CCGAGCAACC AAAGCCTGCC
    ATTCTAACAA AACGTAGACC ACACAGTCAA TGCTTGAATT CCCCGTCTTC CAGTCATCCC
    CATTCCTTTT TCCCTACTTT ACCTTCACCT TCTTCTACTC CTCCTGTGCC AGCTGGTCCT
    GTCACAGATG CTACAAAGCC AAGGTCTCAG GGTTTCACCC CATGTAAACT CTCTTCAGCT
    TCCCCTCAAA CTCAGAGGAA ATTTTCCCTT CAAATACAAA AGCACTATTC AGGCAGTAAA
    GAGCCAGAAA AACTTTCTCC AGTTTTTACT CAAGCAAGAC CTCTGCCATC TCCTCAAATA
    CACAGGCCAA AGCCTTCACG ACCTACCCCA GGGGACCCCG GGAAGCCTGG AGAAGCTCAG
    AAGGGCAGCT TGACTCTTGA CCTGAATGTT TCTCCTTACG ATAACTGTGG CAGTAACGTC
    AGTGCTGTCA TTCCAAGCGA TGATACGGTT TTCACACCTG TAGAGGAGAA GTGCAGACTG
    GATGCAAATA CAGAGCTCAA TTCCAGCATG GAGGATCTGC TGGAAGCTTC GATGCCTGCA
    TGTGATAGTA CTGTCACCTT TAAGTCGGAA GTTGCTGTGC TTTCTTCTGA GCGTGCAGAG
    ACTGACAACA CATACAAAGA TGATGTCAAT CACAATCAGA AATGCAAGGA GAAAATGGAA
    GCTGAGGAGG AAGAGGCTCT GGCTATTGTT TTGGCAATGT CGGCATCTCA GGATGCACTG
    CCAGTCATAC CCCAGCTGCA AGTTCAAAAT GGGGAAGACA TCATCATTAT TCAGCAGGAT
    ACACCAGAGA CTCTGCCTGG ACATACCAAA GCAAGAAATC ATTACAGGGA GGAAATGGAA
    TGGCTCAAGG GCCAGCAGAT TGGCTTAGGA GCGTTTTCTT CCTGCTATCA AGCTCAGGAT
    GTTGGAACCG GAACATTAAT GGCTGTGAAA CAGGTAACCT ATGTGAGGAA CACATCTACT
    GAGCAAGGAG AAGTGGTTGA AGCCCTCCGA GAAGAGATTA GGATGATGAG CCACCTGTCC
    CATCCTAATA TTATACGCAT GTTGGGTGCC ACTTGTGAGA AGAGCAACTA CAATCTGTTT
    ATTGAGTGGA TGGCAGGTGG CTCTGTTGCC CTTTTGCTCA GCAAATATGG AGCATTCAAG
    GAATTGGTTA TCATAAACTA TGCTGAACAA CTGCTACGTG GTCTATCATA CCTGCATGAG
    AATCAGATTA TACACAGAGA TATTAAAGGT GCCAATTTGT TGATTGACAG CACTGGGCAG
    CGGCTGCGAA TTGCTGATTT TGGCGCTGCA GCAAGGTTGG CATCAAAGGG AACTGGTGCG
    GGCGAGTTTC AAGGGCAGCT GCTTGGAACA ATTGCCTTCA TGGCACCTGA GGTGCTGAGA
    GGGCAACAGT ATGGAAGAAG CTGTGATGTG TGGAGTCTTG GCTGCAGCAT AATAGAGATG
    GCATGTGCTA AACCTCCATG GAATGCAGAG AAGCACTCTA ACCATCTTGC ACTTATATTC
    AAGATTGCAA GTGCTACAAC GGCCCCAACG ATCCCACCAC ACCTCTCTCC AGGATTACGG
    GATGTAACCC TCCGATGTCT GGAGCTCCAG CCCCAAGATA GACCACCATC CCGAGAACTA
    CTGAAGCACC CGGTCTTTCG AACAAAGTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750017.1
    CDS16..4068
    Translation

    Target ORF information:

    RefSeq Version XM_031894157.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAATCA ATCTTTGTAG CCGTGACATG GAGAATAAAG AAACACTCAG GGGGCTACAG 
    AAAATGGATG ACCGCCCAGA GGAGCGCATG ATCAGGGAAA AACTCAAGGC AACATGCATG
    CCAGCCTGGA AACATGAATG GTTGGAGAGG AAAAGCAGGC GAGGTCCTGT GGTCGTTAAA
    CCCATTCCTG TAAAAGCCGA GGGGTCTGAA ACTTTCAAGG CAGACTCCGT TGAAGGGTCG
    CTGAATGGCT TGTCCCATGT TCATAAAGGA AGGCGAAGCC CTTCTCCCAG CAGCTCTTCC
    AGCTCATCCA AATCCACAAA GCCTGAATCG CCAGGGTTGA AGAGGAGAGG CCTTTCTCCG
    GTCCCTAGTG GACGTGTTAC ACCACCTCGG AGAGCTCCCT CTCCTGATGG CTTCTCACCT
    TTCAGTCCTG AGGAAACCAG TCGAAGGGTT CACAAAGTTA TGCGCGCAAG ACTTTATCTG
    CTACAGCAGA TTGGACCTAA CTCCTTTCTC ATTGGAGGGG ACAGCCCAGA CAATAAATAC
    AGAGTTTTTA TTGGACCTCA GGCCTGTAGC TGTGGCAGGG GAACATTCTG CATCCATCTT
    CTCTTCGTCA TGTTAAGAGT CTTCCAGTTA GAACCATCTG ACACCATGTT GTGGAGAAAA
    GCACTGAAGA ATTTTGAGGT TGAGAGTTTG TTCCAGAAAT ATCACAGTAG ACGTAGCTCA
    AGGATCAAAG CTCCATCTCG TAACACCATC CAGAAGTTTG TGTCACGCAT GTCCAGTTCA
    CATACATTGT CATCATCTAC CACCTCTACA TCTAGTTCAG ACACCAGCAT GAAAGATGAG
    GAGGAGCAGA TGTGCCCGAT CTGCTTGCTA GGGATGCTGG ACGAGGAGAG TCTGACTGTA
    TGTGAAGAAG GCTGCAGAAA CAAGTTACAC CACCACTGCA TGTCAATTTG GGCAGAAGAG
    TGCAGAAGAA ATAGGGAACC TCTTATCTGT CCTCTTTGTC GAGCAAAGTG GAGGTCGCAG
    GATTTCAACA GCGTCGAATT GCCAGGGCCT GTGGATTCTC CTCCCATTCT TAGGGCAGTT
    CAACAGCAGT CTCTGCAACA GCTGCCTACG CCTGGTTCCC AAAGGAGGCC ACAGGACACA
    AGCCTGAATC TTACACACTA TGGGGTCCAA AATATTCCAT TTGCTTATAA GGACATGGCT
    GAGCCATGGA TTCAGGTTTT CGGAATGGAG CTGGTGAGCT GTTTGTTTTC TCGAAACTGG
    AATATCAGAG AAATGGCCCT GAGGCGCCTC TCCCATGATG TCAGTGGGGC CTTGCTGTTG
    GCAAATGGTG AAAGCACTGG AAATTCATGC AACAGCAGTG GGACTACAAG TGGCACATCC
    CTGAATGCTG CCAGTGGCTC ATCTCAGTCT ACTATCTCAG GGGATGTTGT AGTGGAGGCT
    TGCTGCAGTG TGCTCTCCAT GGTGTGTGCT GACCCAGTCT ACAAGGTGTA CGTCGCAGCT
    TTAAAAACAT TACGGGCCAT GCTGGTGTAC ACTCCCTGCC ATACAATTAC AGAAAGAAAG
    AAGCTGCAGC GACTTCTCAA TCCAGTGGTG GAGACTATTC TTGTAAAATG CGCAGATGCC
    AACAGCCGCA CAAGTCAGCT TTCAGTGTCA ACCTTGTTGG AACTATGCAA AGGACAAGCT
    GGAGAGTTGG CAGTTGGAAG AGAAATACTC AAATCTGGGT CCATCGGCAT TGGTGGGGTG
    GATTATGTCT TGCATTGTAT TCTAGCAACG CAAGCAGAGC CCAATAACTG GCAGTCACTA
    CTTGGCCGCC TTTGTCTGAT CGACAGACTT TTATTGGAGT TTCCTGCTGA ATTTTATCCT
    CACATCATCA GTGGAGACCT CACACAGGCA GATGCAGTCG TTGACAGATA TAAAAGCCTG
    TTGTCCCTGC TGAATTTTGC CTTGCAGTCT ATTGATAACT CTCATTCCAT GGTGGGAAAA
    CTATCCCGGA GAGTATTTTT AAGCGCAGCA AGAATGGTTG CAAGAGTGCC CCAAGTGTTC
    GTCAAGCTGC TGGATATGTT AAGTGTAACT AGCTCTACAC ATTACATTCG TATGCGGCGC
    CGGCTAATGG CCATAGCTGA AGAGATGGAT ATTGTTGAAG TAATCCAGCA GGACGTAGAA
    GATTCTCAAG CAATGGAAAA CCGCAGAGAG GCATTTGTTC AGCCATCTGC CCCAGCCAAT
    ACCCCATCAA CCTCACAAAG CAATTCTCCG GATAATACTG TACAGCTGTC AGGTAAAGTG
    GCAAAAGAAG CGTGTGACTC AAAGTCTGCC TCAAGTCCCG AAGACTTACG CGAGGCACTG
    GAAGTAATGT CATTGGCGTA CCCTTCCTCT TCCGCCACAG CCGAGCAACC AAAGCCTGCC
    ATTCTAACAA AACGTAGACC ACACAGTCAA TGCTTGAATT CCCCGTCTTC CAGTCATCCC
    CATTCCTTTT TCCCTACTTT ACCTTCACCT TCTTCTACTC CTCCTGTGCC AGCTGGTCCT
    GTCACAGATG CTACAAAGCC AAGGTCTCAG GGTTTCACCC CATGTAAACT CTCTTCAGCT
    TCCCCTCAAA CTCAGAGGAA ATTTTCCCTT CAAATACAAA AGCACTATTC AGGCAGTAAA
    GAGCCAGAAA AACTTTCTCC AGTTTTTACT CAAGCAAGAC CTCTGCCATC TCCTCAAATA
    CACAGGCCAA AGCCTTCACG ACCTACCCCA GGGGACCCCG GGAAGCCTGG AGAAGCTCAG
    AAGGGCAGCT TGACTCTTGA CCTGAATGTT TCTCCTTACG ATAACTGTGG CAGTAACGTC
    AGTGCTGTCA TTCCAAGCGA TGATACGGTT TTCACACCTG TAGAGGAGAA GTGCAGACTG
    GATGCAAATA CAGAGCTCAA TTCCAGCATG GAGGATCTGC TGGAAGCTTC GATGCCTGCA
    TGTGATAGTA CTGTCACCTT TAAGTCGGAA GTTGCTGTGC TTTCTTCTGA GCGTGCAGAG
    ACTGACAACA CATACAAAGA TGATGTCAAT CACAATCAGA AATGCAAGGA GAAAATGGAA
    GCTGAGGAGG AAGAGGCTCT GGCTATTGTT TTGGCAATGT CGGCATCTCA GGATGCACTG
    CCAGTCATAC CCCAGCTGCA AGTTCAAAAT GGGGAAGACA TCATCATTAT TCAGCAGGAT
    ACACCAGAGA CTCTGCCTGG ACATACCAAA GCAAGAAATC ATTACAGGGA GGAAATGGAA
    TGGCTCAAGG GCCAGCAGAT TGGCTTAGGA GCGTTTTCTT CCTGCTATCA AGCTCAGGAT
    GTTGGAACCG GAACATTAAT GGCTGTGAAA CAGGTAACCT ATGTGAGGAA CACATCTACT
    GAGCAAGGAG AAGTGGTTGA AGCCCTCCGA GAAGAGATTA GGATGATGAG CCACCTGTCC
    CATCCTAATA TTATACGCAT GTTGGGTGCC ACTTGTGAGA AGAGCAACTA CAATCTGTTT
    ATTGAGTGGA TGGCAGGTGG CTCTGTTGCC CTTTTGCTCA GCAAATATGG AGCATTCAAG
    GAATTGGTTA TCATAAACTA TGCTGAACAA CTGCTACGTG GTCTATCATA CCTGCATGAG
    AATCAGATTA TACACAGAGA TATTAAAGGT GCCAATTTGT TGATTGACAG CACTGGGCAG
    CGGCTGCGAA TTGCTGATTT TGGCGCTGCA GCAAGGTTGG CATCAAAGGG AACTGGTGCG
    GGCGAGTTTC AAGGGCAGCT GCTTGGAACA ATTGCCTTCA TGGCACCTGA GGTGCTGAGA
    GGGCAACAGT ATGGAAGAAG CTGTGATGTG TGGAGTCTTG GCTGCAGCAT AATAGAGATG
    GCATGTGCTA AACCTCCATG GAATGCAGAG AAGCACTCTA ACCATCTTGC ACTTATATTC
    AAGATTGCAA GTGCTACAAC GGCCCCAACG ATCCCACCAC ACCTCTCTCC AGGATTACGG
    GATGTAACCC TCCGATGTCT GGAGCTCCAG CCCCAAGATA GACCACCATC CCGAGAACTA
    CTGAAGCACC CGGTCTTTCG AACAAAGTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34788
    Clone ID Related Accession (Same CDS sequence) XM_012966894.2 , XM_012966894.3
    Accession Version XM_012966894.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCCG CGGCGGGGAG TCGGAGCGTG TCGTCTGGAT TAACGGCAGC CGCGGGCGGC 
    GTAGCGGGCT CCGGTAGCCC GGAGACAGGG GGGCAAAGAC TTCCAGAAGG GGCTCCGGGG
    GAGGCAGGGG GCGAGCAGCG AGCCGACTGG AGGCGCAAGC AGCTGCGCAA AGTTCGGAGT
    GTGGAAGTGG ATAAGCTGGC GGGGAGAGAG CCGGAACCTC CGCACGTATT GGCTGCCGGA
    GTGAGGGAAC AGGAGCCTGC TGCGGGGAGC GAACCCGAGG AGCCTTCGGC GGCACACAGC
    TCATGCCGTG ACATGGAGAA TAAAGAAACA CTCAGGGGGC TACAGAAAAT GGATGACCGC
    CCAGAGGAGC GCATGATCAG GGAAAAACTC AAGGCAACAT GCATGCCAGC CTGGAAACAT
    GAATGGTTGG AGAGGAAAAG CAGGCGAGGT CCTGTGGTCG TTAAACCCAT TCCTGTAAAA
    GCCGAGGGGT CTGAAACTTT CAAGGCAGAC TCCGTTGAAG GGTCGCTGAA TGGCTTGTCC
    CATGTTCATA AAGGAAGGCG AAGCCCTTCT CCCAGCAGCT CTTCCAGCTC ATCCAAATCC
    ACAAAGCCTG AATCGCCAGG GTTGAAGAGG AGAGGCCTTT CTCCGGTCCC TAGTGGACGT
    GTTACACCAC CTCGGAGAGC TCCCTCTCCT GATGGCTTCT CACCTTTCAG TCCTGAGGAA
    ACCAGTCGAA GGGTTCACAA AGTTATGCGC GCAAGACTTT ATCTGCTACA GCAGATTGGA
    CCTAACTCCT TTCTCATTGG AGGGGACAGC CCAGACAATA AATACAGAGT TTTTATTGGA
    CCTCAGGCCT GTAGCTGTGG CAGGGGAACA TTCTGCATCC ATCTTCTCTT CGTCATGTTA
    AGAGTCTTCC AGTTAGAACC ATCTGACACC ATGTTGTGGA GAAAAGCACT GAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGACGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTGTCA CGCATGTCCA GTTCACATAC ATTGTCATCA
    TCTACCACCT CTACATCTAG TTCAGACACC AGCATGAAAG ATGAGGAGGA GCAGATGTGC
    CCGATCTGCT TGCTAGGGAT GCTGGACGAG GAGAGTCTGA CTGTATGTGA AGAAGGCTGC
    AGAAACAAGT TACACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGCAG AAGAAATAGG
    GAACCTCTTA TCTGTCCTCT TTGTCGAGCA AAGTGGAGGT CGCAGGATTT CAACAGCGTC
    GAATTGCCAG GGCCTGTGGA TTCTCCTCCC ATTCTTAGGG CAGTTCAACA GCAGTCTCTG
    CAACAGCTGC CTACGCCTGG TTCCCAAAGG AGGCCACAGG ACACAAGCCT GAATCTTACA
    CACTATGGGG TCCAAAATAT TCCATTTGCT TATAAGGACA TGGCTGAGCC ATGGATTCAG
    GTTTTCGGAA TGGAGCTGGT GAGCTGTTTG TTTTCTCGAA ACTGGAATAT CAGAGAAATG
    GCCCTGAGGC GCCTCTCCCA TGATGTCAGT GGGGCCTTGC TGTTGGCAAA TGGTGAAAGC
    ACTGGAAATT CATGCAACAG CAGTGGGACT ACAAGTGGCA CATCCCTGAA TGCTGCCAGT
    GGCTCATCTC AGTCTACTAT CTCAGGGGAT GTTGTAGTGG AGGCTTGCTG CAGTGTGCTC
    TCCATGGTGT GTGCTGACCC AGTCTACAAG GTGTACGTCG CAGCTTTAAA AACATTACGG
    GCCATGCTGG TGTACACTCC CTGCCATACA ATTACAGAAA GAAAGAAGCT GCAGCGACTT
    CTCAATCCAG TGGTGGAGAC TATTCTTGTA AAATGCGCAG ATGCCAACAG CCGCACAAGT
    CAGCTTTCAG TGTCAACCTT GTTGGAACTA TGCAAAGGAC AAGCTGGAGA GTTGGCAGTT
    GGAAGAGAAA TACTCAAATC TGGGTCCATC GGCATTGGTG GGGTGGATTA TGTCTTGCAT
    TGTATTCTAG CAACGCAAGC AGAGCCCAAT AACTGGCAGT CACTACTTGG CCGCCTTTGT
    CTGATCGACA GACTTTTATT GGAGTTTCCT GCTGAATTTT ATCCTCACAT CATCAGTGGA
    GACCTCACAC AGGCAGATGC AGTCGTTGAC AGATATAAAA GCCTGTTGTC CCTGCTGAAT
    TTTGCCTTGC AGTCTATTGA TAACTCTCAT TCCATGGTGG GAAAACTATC CCGGAGAGTA
    TTTTTAAGCG CAGCAAGAAT GGTTGCAAGA GTGCCCCAAG TGTTCGTCAA GCTGCTGGAT
    ATGTTAAGTG TAACTAGCTC TACACATTAC ATTCGTATGC GGCGCCGGCT AATGGCCATA
    GCTGAAGAGA TGGATATTGT TGAAGTAATC CAGCAGGACG TAGAAGATTC TCAAGCAATG
    GAAAACCGCA GAGAGGCATT TGTTCAGCCA TCTGCCCCAG CCAATACCCC ATCAACCTCA
    CAAAGCAATT CTCCGGATAA TACTGTACAG CTGTCAGGTA AAGTGGCAAA AGAAGCGTGT
    GACTCAAAGT CTGCCTCAAG TCCCGAAGAC TTACGCGAGG CACTGGAAGT AATGTCATTG
    GCGTACCCTT CCTCTTCCGC CACAGCCGAG CAACCAAAGC CTGCCATTCT AACAAAACGT
    AGACCACACA GTCAATGCTT GAATTCCCCG TCTTCCAGTC ATCCCCATTC CTTTTTCCCT
    ACTTTACCTT CACCTTCTTC TACTCCTCCT GTGCCAGCTG GTCCTGTCAC AGATGCTACA
    AAGCCAAGGT CTCAGGGTTT CACCCCATGT AAACTCTCTT CAGCTTCCCC TCAAACTCAG
    AGGAAATTTT CCCTTCAAAT ACAAAAGCAC TATTCAGGCA GTAAAGAGCC AGAAAAACTT
    TCTCCAGTTT TTACTCAAGC AAGACCTCTG CCATCTCCTC AAATACACAG GCCAAAGCCT
    TCACGACCTA CCCCAGGGGA CCCCGGGAAG CCTGGAGAAG CTCAGAAGGG CAGCTTGACT
    CTTGACCTGA ATGTTTCTCC TTACGATAAC TGTGGCAGTA ACGTCAGTGC TGTCATTCCA
    AGCGATGATA CGGTTTTCAC ACCTGTAGAG GAGAAGTGCA GACTGGATGC AAATACAGAG
    CTCAATTCCA GCATGGAGGA TCTGCTGGAA GCTTCGATGC CTGCATGTGA TAGTACTGTC
    ACCTTTAAGT CGGAAGTTGC TGTGCTTTCT TCTGAGCGTG CAGAGACTGA CAACACATAC
    AAAGATGATG TCAATCACAA TCAGAAATGC AAGGAGAAAA TGGAAGCTGA GGAGGAAGAG
    GCTCTGGCTA TTGTTTTGGC AATGTCGGCA TCTCAGGATG CACTGCCAGT CATACCCCAG
    CTGCAAGTTC AAAATGGGGA AGACATCATC ATTATTCAGC AGGATACACC AGAGACTCTG
    CCTGGACATA CCAAAGCAAG AAATCATTAC AGGGAGGAAA TGGAATGGCT CAAGGGCCAG
    CAGATTGGCT TAGGAGCGTT TTCTTCCTGC TATCAAGCTC AGGATGTTGG AACCGGAACA
    TTAATGGCTG TGAAACAGGT AACCTATGTG AGGAACACAT CTACTGAGCA AGGAGAAGTG
    GTTGAAGCCC TCCGAGAAGA GATTAGGATG ATGAGCCACC TGTCCCATCC TAATATTATA
    CGCATGTTGG GTGCCACTTG TGAGAAGAGC AACTACAATC TGTTTATTGA GTGGATGGCA
    GGTGGCTCTG TTGCCCTTTT GCTCAGCAAA TATGGAGCAT TCAAGGAATT GGTTATCATA
    AACTATGCTG AACAACTGCT ACGTGGTCTA TCATACCTGC ATGAGAATCA GATTATACAC
    AGAGATATTA AAGGTGCCAA TTTGTTGATT GACAGCACTG GGCAGCGGCT GCGAATTGCT
    GATTTTGGCG CTGCAGCAAG GTTGGCATCA AAGGGAACTG GTGCGGGCGA GTTTCAAGGG
    CAGCTGCTTG GAACAATTGC CTTCATGGCA CCTGAGGTGC TGAGAGGGCA ACAGTATGGA
    AGAAGCTGTG ATGTGTGGAG TCTTGGCTGC AGCATAATAG AGATGGCATG TGCTAAACCT
    CCATGGAATG CAGAGAAGCA CTCTAACCAT CTTGCACTTA TATTCAAGAT TGCAAGTGCT
    ACAACGGCCC CAACGATCCC ACCACACCTC TCTCCAGGAT TACGGGATGT AACCCTCCGA
    TGTCTGGAGC TCCAGCCCCA AGATAGACCA CCATCCCGAG AACTACTGAA GCACCCGGTC
    TTTCGAACAA AGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822348.1
    CDS254..4591
    Translation

    Target ORF information:

    RefSeq Version XM_012966894.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (map3k1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCCG CGGCGGGGAG TCGGAGCGTG TCGTCTGGAT TAACGGCAGC CGCGGGCGGC 
    GTAGCGGGCT CCGGTAGCCC GGAGACAGGG GGGCAAAGAC TTCCAGAAGG GGCTCCGGGG
    GAGGCAGGGG GCGAGCAGCG AGCCGACTGG AGGCGCAAGC AGCTGCGCAA AGTTCGGAGT
    GTGGAAGTGG ATAAGCTGGC GGGGAGAGAG CCGGAACCTC CGCACGTATT GGCTGCCGGA
    GTGAGGGAAC AGGAGCCTGC TGCGGGGAGC GAACCCGAGG AGCCTTCGGC GGCACACAGC
    TCATGCCGTG ACATGGAGAA TAAAGAAACA CTCAGGGGGC TACAGAAAAT GGATGACCGC
    CCAGAGGAGC GCATGATCAG GGAAAAACTC AAGGCAACAT GCATGCCAGC CTGGAAACAT
    GAATGGTTGG AGAGGAAAAG CAGGCGAGGT CCTGTGGTCG TTAAACCCAT TCCTGTAAAA
    GCCGAGGGGT CTGAAACTTT CAAGGCAGAC TCCGTTGAAG GGTCGCTGAA TGGCTTGTCC
    CATGTTCATA AAGGAAGGCG AAGCCCTTCT CCCAGCAGCT CTTCCAGCTC ATCCAAATCC
    ACAAAGCCTG AATCGCCAGG GTTGAAGAGG AGAGGCCTTT CTCCGGTCCC TAGTGGACGT
    GTTACACCAC CTCGGAGAGC TCCCTCTCCT GATGGCTTCT CACCTTTCAG TCCTGAGGAA
    ACCAGTCGAA GGGTTCACAA AGTTATGCGC GCAAGACTTT ATCTGCTACA GCAGATTGGA
    CCTAACTCCT TTCTCATTGG AGGGGACAGC CCAGACAATA AATACAGAGT TTTTATTGGA
    CCTCAGGCCT GTAGCTGTGG CAGGGGAACA TTCTGCATCC ATCTTCTCTT CGTCATGTTA
    AGAGTCTTCC AGTTAGAACC ATCTGACACC ATGTTGTGGA GAAAAGCACT GAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATATCAC AGTAGACGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTGTCA CGCATGTCCA GTTCACATAC ATTGTCATCA
    TCTACCACCT CTACATCTAG TTCAGACACC AGCATGAAAG ATGAGGAGGA GCAGATGTGC
    CCGATCTGCT TGCTAGGGAT GCTGGACGAG GAGAGTCTGA CTGTATGTGA AGAAGGCTGC
    AGAAACAAGT TACACCACCA CTGCATGTCA ATTTGGGCAG AAGAGTGCAG AAGAAATAGG
    GAACCTCTTA TCTGTCCTCT TTGTCGAGCA AAGTGGAGGT CGCAGGATTT CAACAGCGTC
    GAATTGCCAG GGCCTGTGGA TTCTCCTCCC ATTCTTAGGG CAGTTCAACA GCAGTCTCTG
    CAACAGCTGC CTACGCCTGG TTCCCAAAGG AGGCCACAGG ACACAAGCCT GAATCTTACA
    CACTATGGGG TCCAAAATAT TCCATTTGCT TATAAGGACA TGGCTGAGCC ATGGATTCAG
    GTTTTCGGAA TGGAGCTGGT GAGCTGTTTG TTTTCTCGAA ACTGGAATAT CAGAGAAATG
    GCCCTGAGGC GCCTCTCCCA TGATGTCAGT GGGGCCTTGC TGTTGGCAAA TGGTGAAAGC
    ACTGGAAATT CATGCAACAG CAGTGGGACT ACAAGTGGCA CATCCCTGAA TGCTGCCAGT
    GGCTCATCTC AGTCTACTAT CTCAGGGGAT GTTGTAGTGG AGGCTTGCTG CAGTGTGCTC
    TCCATGGTGT GTGCTGACCC AGTCTACAAG GTGTACGTCG CAGCTTTAAA AACATTACGG
    GCCATGCTGG TGTACACTCC CTGCCATACA ATTACAGAAA GAAAGAAGCT GCAGCGACTT
    CTCAATCCAG TGGTGGAGAC TATTCTTGTA AAATGCGCAG ATGCCAACAG CCGCACAAGT
    CAGCTTTCAG TGTCAACCTT GTTGGAACTA TGCAAAGGAC AAGCTGGAGA GTTGGCAGTT
    GGAAGAGAAA TACTCAAATC TGGGTCCATC GGCATTGGTG GGGTGGATTA TGTCTTGCAT
    TGTATTCTAG CAACGCAAGC AGAGCCCAAT AACTGGCAGT CACTACTTGG CCGCCTTTGT
    CTGATCGACA GACTTTTATT GGAGTTTCCT GCTGAATTTT ATCCTCACAT CATCAGTGGA
    GACCTCACAC AGGCAGATGC AGTCGTTGAC AGATATAAAA GCCTGTTGTC CCTGCTGAAT
    TTTGCCTTGC AGTCTATTGA TAACTCTCAT TCCATGGTGG GAAAACTATC CCGGAGAGTA
    TTTTTAAGCG CAGCAAGAAT GGTTGCAAGA GTGCCCCAAG TGTTCGTCAA GCTGCTGGAT
    ATGTTAAGTG TAACTAGCTC TACACATTAC ATTCGTATGC GGCGCCGGCT AATGGCCATA
    GCTGAAGAGA TGGATATTGT TGAAGTAATC CAGCAGGACG TAGAAGATTC TCAAGCAATG
    GAAAACCGCA GAGAGGCATT TGTTCAGCCA TCTGCCCCAG CCAATACCCC ATCAACCTCA
    CAAAGCAATT CTCCGGATAA TACTGTACAG CTGTCAGGTA AAGTGGCAAA AGAAGCGTGT
    GACTCAAAGT CTGCCTCAAG TCCCGAAGAC TTACGCGAGG CACTGGAAGT AATGTCATTG
    GCGTACCCTT CCTCTTCCGC CACAGCCGAG CAACCAAAGC CTGCCATTCT AACAAAACGT
    AGACCACACA GTCAATGCTT GAATTCCCCG TCTTCCAGTC ATCCCCATTC CTTTTTCCCT
    ACTTTACCTT CACCTTCTTC TACTCCTCCT GTGCCAGCTG GTCCTGTCAC AGATGCTACA
    AAGCCAAGGT CTCAGGGTTT CACCCCATGT AAACTCTCTT CAGCTTCCCC TCAAACTCAG
    AGGAAATTTT CCCTTCAAAT ACAAAAGCAC TATTCAGGCA GTAAAGAGCC AGAAAAACTT
    TCTCCAGTTT TTACTCAAGC AAGACCTCTG CCATCTCCTC AAATACACAG GCCAAAGCCT
    TCACGACCTA CCCCAGGGGA CCCCGGGAAG CCTGGAGAAG CTCAGAAGGG CAGCTTGACT
    CTTGACCTGA ATGTTTCTCC TTACGATAAC TGTGGCAGTA ACGTCAGTGC TGTCATTCCA
    AGCGATGATA CGGTTTTCAC ACCTGTAGAG GAGAAGTGCA GACTGGATGC AAATACAGAG
    CTCAATTCCA GCATGGAGGA TCTGCTGGAA GCTTCGATGC CTGCATGTGA TAGTACTGTC
    ACCTTTAAGT CGGAAGTTGC TGTGCTTTCT TCTGAGCGTG CAGAGACTGA CAACACATAC
    AAAGATGATG TCAATCACAA TCAGAAATGC AAGGAGAAAA TGGAAGCTGA GGAGGAAGAG
    GCTCTGGCTA TTGTTTTGGC AATGTCGGCA TCTCAGGATG CACTGCCAGT CATACCCCAG
    CTGCAAGTTC AAAATGGGGA AGACATCATC ATTATTCAGC AGGATACACC AGAGACTCTG
    CCTGGACATA CCAAAGCAAG AAATCATTAC AGGGAGGAAA TGGAATGGCT CAAGGGCCAG
    CAGATTGGCT TAGGAGCGTT TTCTTCCTGC TATCAAGCTC AGGATGTTGG AACCGGAACA
    TTAATGGCTG TGAAACAGGT AACCTATGTG AGGAACACAT CTACTGAGCA AGGAGAAGTG
    GTTGAAGCCC TCCGAGAAGA GATTAGGATG ATGAGCCACC TGTCCCATCC TAATATTATA
    CGCATGTTGG GTGCCACTTG TGAGAAGAGC AACTACAATC TGTTTATTGA GTGGATGGCA
    GGTGGCTCTG TTGCCCTTTT GCTCAGCAAA TATGGAGCAT TCAAGGAATT GGTTATCATA
    AACTATGCTG AACAACTGCT ACGTGGTCTA TCATACCTGC ATGAGAATCA GATTATACAC
    AGAGATATTA AAGGTGCCAA TTTGTTGATT GACAGCACTG GGCAGCGGCT GCGAATTGCT
    GATTTTGGCG CTGCAGCAAG GTTGGCATCA AAGGGAACTG GTGCGGGCGA GTTTCAAGGG
    CAGCTGCTTG GAACAATTGC CTTCATGGCA CCTGAGGTGC TGAGAGGGCA ACAGTATGGA
    AGAAGCTGTG ATGTGTGGAG TCTTGGCTGC AGCATAATAG AGATGGCATG TGCTAAACCT
    CCATGGAATG CAGAGAAGCA CTCTAACCAT CTTGCACTTA TATTCAAGAT TGCAAGTGCT
    ACAACGGCCC CAACGATCCC ACCACACCTC TCTCCAGGAT TACGGGATGT AACCCTCCGA
    TGTCTGGAGC TCCAGCCCCA AGATAGACCA CCATCCCGAG AACTACTGAA GCACCCGGTC
    TTTCGAACAA AGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.