ibtk cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ibtk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ibtk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa58447 XM_031902137.1
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58447 XM_031902138.1
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58447 XM_031902136.1
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58447 XM_031902139.1
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa34691 XM_018094321.1
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa10406 XM_004914524.3
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10406 XM_002938457.4
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10406 XM_012963507.2
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10406 XM_004914523.3
Latest version!
Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa58447
    Clone ID Related Accession (Same CDS sequence) XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
    Accession Version XM_031902137.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757997.1
    CDS668..4681
    Translation

    Target ORF information:

    RefSeq Version XM_031902137.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902137.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58447
    Clone ID Related Accession (Same CDS sequence) XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
    Accession Version XM_031902139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757999.1
    CDS433..4446
    Translation

    Target ORF information:

    RefSeq Version XM_031902139.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58447
    Clone ID Related Accession (Same CDS sequence) XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
    Accession Version XM_031902136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757996.1
    CDS440..4453
    Translation

    Target ORF information:

    RefSeq Version XM_031902136.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58447
    Clone ID Related Accession (Same CDS sequence) XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
    Accession Version XM_031902138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757998.1
    CDS447..4460
    Translation

    Target ORF information:

    RefSeq Version XM_031902138.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34691
    Clone ID Related Accession (Same CDS sequence) XM_018094321.1
    Accession Version XM_018094321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGT CCTTGGCATT TGACATTGAG CAAAAATACC
    TCCTCATCCG CCCCTGTGAC TTTTACTGCC ATTGTAGAGG AAGAGGAGAA ACAAGAAGCT
    GCTCTCATCA GAAGTCGAGA AAAGCCACTG GCATTAATTC AGATAGAAGA ACGAGCAATC
    CAAGATTTGT TAATACACTA CCAGGCATTA GACAACCCTG AAGAATACAT TACTGTTGAG
    AGGGCCGCAC AGACACCTAT GGCAACACCA ATGTGGAACA AACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949810.1
    CDS874..4821
    Translation

    Target ORF information:

    RefSeq Version XM_018094321.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGT CCTTGGCATT TGACATTGAG CAAAAATACC
    TCCTCATCCG CCCCTGTGAC TTTTACTGCC ATTGTAGAGG AAGAGGAGAA ACAAGAAGCT
    GCTCTCATCA GAAGTCGAGA AAAGCCACTG GCATTAATTC AGATAGAAGA ACGAGCAATC
    CAAGATTTGT TAATACACTA CCAGGCATTA GACAACCCTG AAGAATACAT TACTGTTGAG
    AGGGCCGCAC AGACACCTAT GGCAACACCA ATGTGGAACA AACATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10406
    Clone ID Related Accession (Same CDS sequence) XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
    Accession Version XM_004914524.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914581.1
    CDS403..4416
    Translation

    Target ORF information:

    RefSeq Version XM_004914524.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914524.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10406
    Clone ID Related Accession (Same CDS sequence) XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
    Accession Version XM_012963507.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818961.1
    CDS653..4666
    Translation

    Target ORF information:

    RefSeq Version XM_012963507.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963507.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10406
    Clone ID Related Accession (Same CDS sequence) XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
    Accession Version XM_004914523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914580.1
    CDS874..4887
    Translation

    Target ORF information:

    RefSeq Version XM_004914523.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10406
    Clone ID Related Accession (Same CDS sequence) XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
    Accession Version XM_002938457.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product inhibitor of Bruton tyrosine kinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002938457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938503.2
    CDS439..4452
    Translation

    Target ORF information:

    RefSeq Version XM_002938457.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938457.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
    GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC
    TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA
    TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA
    GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT
    GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA
    TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC
    GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT
    CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC
    AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC
    ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT
    CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT
    CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT
    CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA
    ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG
    ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT
    CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC
    ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA
    GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG
    AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG
    GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC
    TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT
    ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA
    TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA
    TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC
    CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT
    GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT
    TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA
    GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC
    GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG
    GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT
    GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG
    CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG
    GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC
    AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT
    TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT
    AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA
    AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC
    ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA
    TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG
    CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT
    GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG
    GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA
    ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG
    CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG
    TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG
    ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC
    TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA
    AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA
    CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA
    GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT
    GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC
    TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC
    CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA
    GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC
    TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC
    AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT
    GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT
    AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA
    TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA
    AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA
    GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA
    TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA
    AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA
    GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA
    GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT
    GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.