ibtk gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004914524.3	XP_004914581.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_031902137.1	XP_031757997.1	inhibitor of Bruton tyrosine kinase
XM_031902139.1	XP_031757999.1	inhibitor of Bruton tyrosine kinase
XM_012963507.2	XP_012818961.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_018094321.1	XP_017949810.1	inhibitor of Bruton tyrosine kinase isoform X2
XM_004914523.3	XP_004914580.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_002938457.4	XP_002938503.2	inhibitor of Bruton tyrosine kinase isoform X1
XM_031902136.1	XP_031757996.1	inhibitor of Bruton tyrosine kinase
XM_031902138.1	XP_031757998.1	inhibitor of Bruton tyrosine kinase

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58447	XM_031902137.1 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58447	XM_031902138.1 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58447	XM_031902136.1 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58447	XM_031902139.1 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa34691	XM_018094321.1 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa10406	XM_004914524.3 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa10406	XM_002938457.4 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa10406	XM_012963507.2 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa10406	XM_004914523.3 Latest version!	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ibtk ( XM_031902137.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757997.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa58447
Clone ID Related Accession (Same CDS sequence)	XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
Accession Version	XM_031902137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757997.1
CDS	668..4681
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIMCHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_031902137.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902137.1

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_031902139.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757999.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa58447
Clone ID Related Accession (Same CDS sequence)	XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
Accession Version	XM_031902139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757999.1
CDS	433..4446
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIMCHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_031902139.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902139.1

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_031902136.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757996.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

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CloneID	OXa58447
Clone ID Related Accession (Same CDS sequence)	XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
Accession Version	XM_031902136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757996.1
CDS	440..4453
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIMCHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_031902136.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902136.1

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_031902138.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757998.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58447
Clone ID Related Accession (Same CDS sequence)	XM_031902137.1 , XM_031902139.1 , XM_031902136.1 , XM_031902138.1
Accession Version	XM_031902138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757998.1
CDS	447..4460
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIMCHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_031902138.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902138.1

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCTACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATG 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAA GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTT 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_018094321.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949810.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa34691
Clone ID Related Accession (Same CDS sequence)	XM_018094321.1
Accession Version	XM_018094321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGT CCTTGGCATT TGACATTGAG CAAAAATACC 
TCCTCATCCG CCCCTGTGAC TTTTACTGCC ATTGTAGAGG AAGAGGAGAA ACAAGAAGCT 
GCTCTCATCA GAAGTCGAGA AAAGCCACTG GCATTAATTC AGATAGAAGA ACGAGCAATC 
CAAGATTTGT TAATACACTA CCAGGCATTA GACAACCCTG AAGAATACAT TACTGTTGAG 
AGGGCCGCAC AGACACCTAT GGCAACACCA ATGTGGAACA AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949810.1
CDS	874..4821
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIICHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPASPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_018094321.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094321.1

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGT CCTTGGCATT TGACATTGAG CAAAAATACC 
TCCTCATCCG CCCCTGTGAC TTTTACTGCC ATTGTAGAGG AAGAGGAGAA ACAAGAAGCT 
GCTCTCATCA GAAGTCGAGA AAAGCCACTG GCATTAATTC AGATAGAAGA ACGAGCAATC 
CAAGATTTGT TAATACACTA CCAGGCATTA GACAACCCTG AAGAATACAT TACTGTTGAG 
AGGGCCGCAC AGACACCTAT GGCAACACCA ATGTGGAACA AACATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_004914524.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914581.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

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CloneID	OXa10406
Clone ID Related Accession (Same CDS sequence)	XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
Accession Version	XM_004914524.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914581.1
CDS	403..4416
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIICHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_004914524.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914524.3

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_012963507.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818961.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10406
Clone ID Related Accession (Same CDS sequence)	XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
Accession Version	XM_012963507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818961.1
CDS	653..4666
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIICHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_012963507.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963507.2

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_004914523.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914580.1 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10406
Clone ID Related Accession (Same CDS sequence)	XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
Accession Version	XM_004914523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914580.1
CDS	874..4887
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIICHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_004914523.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914523.3

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ibtk ( XM_002938457.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002938503.2 Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

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CloneID	OXa10406
Clone ID Related Accession (Same CDS sequence)	XM_004914524.3 , XM_012963507.2 , XM_004914523.3 , XM_002938457.4
Accession Version	XM_002938457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002938457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002938503.2
CDS	439..4452
Translation	MERVWPECTTRCRSLQHALDVISVVTRGSEQQIKIFLSSYCCNATTLKDTFGRNVTHIAASCGKKNVLDWLVTEKGVDLSVKDKESGWTALHRSIFYGHIDCALSLLKHGSNLYIQDKDGYTPLDVVMKDRPPHIIFKTSDPTEVYTWGDNVNFTLGHGTQQSKHHPELLETFPRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGQGGRLGHGDELTCLVPRLVEGLSTHPCAQVAAAKDHTVVLSEDGYVYTFGLNTFHQLGIQPPPPNSNVPKQVQAKTMKGKTVLGVAAGRFHTVLWTKDAVYTVGLNGGQLGYLQDPNGEKCVSCPRQVSALHHKDINITLVSASDGATVCVSERGDIYLLSEYQCKKLASKQLNLKKVLVSGGFLEYKAAPEHLKDNGGQPACVLALDQAGRVFCWKSPGSSLKQCRWVYGRQVFMSDVALNKHEIMFVTQDGEGFTGKWMLEKKKKENTISSIICHFDPQNLFERINLQKLPFVHRAVSVATDPSGCNFAVLQSDPKTSLFQVPSVSSSVFAEDFENFLNEVNETDSIHDVTFQVGTKIFPVHKYILALRSDFFGKLFSSGEINCLDFPEVHQKGEDAAGCDLFVIEKIPPELFEYVLQFIYFDSCDMLLQGHKPKLRHKEENEDTILPTFQKMGFHEEIKGKSAYEVYKNSRISAENEKQKGKTKQSKKTKSICDETSPVKLLQNTAKKFGLTNLISRLDGVRYENGRINVFQKKSGDKLRFNQEKCSSLYDVMMKSEDGKEFHCHKCVLCARLEYFNSMLSSSWIEATCCSKLQMPIHSDVLQVILDYIYTDEALAVKESANVEFICNVLVMADQLLIVRLKEICEVTIAERITLKNAAELLEIAALYNADQLKLSCLQFIVLNMGALLEARSLDVLSDDVLKDLSEAYRKMIPPMNKRIITPYLDGPDVSILQSEDIKSLVSVQDDIYSYQNTQEAAFKKSKAKAKKKPRKRLDSSGGYNLSDIIHSSPSTGFVKPEKTNSVESLQDLLTSDSEGSFVGASSPRDLQSPDFFPTFTPEAKETICVEINGSSPPVTNRAASKKKPKPTPSSPEVIPSSRITPSSSPNWVAMPFSPSSPVAVDLRAIMELEENKQKCGAMPKLNSGGPKPVAHGMKLSQKQRKMMAMSSKESNNDNKPAIITATASIKSPAKTWAAAFHFGECKSFRELLLEENQSVISFPSLGSDVKKTKHTEELDPPEPARLSGSISHEAKLKCDVPNQDSSNPWHLTLSKNTSSSAPVTFTAIVEEEEKQEAALIRSREKPLALIQIEERAIQDLLIHYQALDNPEEYITVERAAQTPMATPMWNKH

Target ORF information:

RefSeq Version	XM_002938457.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis inhibitor of Bruton tyrosine kinase (ibtk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002938457.4

ORF Insert Sequence:

ATGGAAAGAG TATGGCCTGA GTGCACTACA CGTTGTCGCT CTCTCCAGCA TGCCTTGGAT 
GTTATTTCCG TGGTGACTAG AGGAAGTGAA CAGCAGATAA AGATCTTCCT TTCTTCTTAC 
TGCTGTAATG CTACTACACT AAAAGATACC TTTGGGCGGA ATGTTACTCA TATTGCAGCA 
TCCTGTGGGA AGAAAAATGT ACTTGATTGG TTAGTTACAG AAAAAGGTGT TGACTTGTCA 
GTCAAAGACA AAGAATCAGG ATGGACAGCA CTACACAGAA GCATTTTCTA CGGACATATT 
GATTGTGCAT TATCCCTCTT AAAGCATGGC AGTAACCTTT ACATTCAAGA TAAAGATGGA 
TATACTCCGT TGGATGTGGT AATGAAAGAC AGACCTCCCC ACATTATTTT CAAAACTAGC 
GATCCAACTG AGGTTTATAC ATGGGGGGAC AATGTAAATT TCACTTTGGG ACATGGTACT 
CAACAAAGCA AGCACCACCC TGAGCTATTG GAAACGTTTC CTCGTTCTGG AGTGTATATC 
AAGCAGGTGG TACTTTGCAA ATTTCACTCA GTCTTCCTGT CTCAGAAAGG ACAGGTTTAC 
ACATGTGGAC ATGGACAAGG AGGTCGCCTT GGTCATGGAG ATGAACTTAC ATGCTTGGTT 
CCTAGGCTAG TGGAAGGTCT TAGCACTCAT CCATGTGCTC AGGTGGCAGC AGCAAAGGAT 
CATACAGTAG TGCTATCTGA AGATGGATAT GTTTACACAT TTGGGCTTAA TACATTTCAT 
CAACTTGGAA TTCAGCCGCC CCCTCCAAAT TCAAATGTTC CAAAGCAGGT TCAGGCCAAA 
ACCATGAAAG GAAAGACTGT GTTAGGGGTC GCTGCTGGAA GGTTTCACAC AGTTTTGTGG 
ACTAAAGATG CTGTATATAC AGTGGGACTG AATGGTGGTC AGTTAGGATA CTTACAAGAT 
CCCAATGGAG AAAAATGTGT AAGTTGTCCT CGCCAAGTAT CCGCACTCCA TCACAAAGAC 
ATAAATATAA CTCTTGTGTC TGCAAGTGAT GGGGCTACAG TTTGTGTCAG TGAAAGGGGA 
GATATTTACC TTCTGTCTGA ATATCAATGC AAAAAGCTGG CATCAAAGCA GTTAAACCTG 
AAGAAAGTTC TTGTTTCGGG TGGATTTTTG GAATATAAGG CAGCTCCTGA GCATCTTAAG 
GACAATGGGG GCCAACCTGC TTGTGTTCTT GCTCTGGATC AAGCTGGAAG AGTTTTTTGC 
TGGAAGTCTC CTGGCAGTTC ACTAAAACAG TGCCGCTGGG TTTATGGTCG CCAAGTATTT 
ATGTCCGATG TTGCTTTAAA TAAGCATGAA ATCATGTTTG TAACGCAAGA TGGTGAAGGA 
TTTACTGGAA AGTGGATGCT AGAAAAGAAA AAGAAAGAGA ATACAATTTC CAGTATAATA 
TGCCACTTTG ACCCTCAGAA TTTGTTTGAA AGAATTAACT TGCAGAAGCT TCCCTTTGTC 
CATCGGGCTG TTAGTGTTGC TACAGACCCC AGTGGATGTA ACTTTGCTGT GTTACAGTCT 
GATCCCAAAA CAAGCCTTTT TCAGGTGCCT TCTGTGTCTT CCTCTGTGTT TGCTGAAGAT 
TTTGAGAATT TTTTGAATGA AGTGAATGAA ACAGATAGTA TTCATGATGT CACTTTCCAA 
GTTGGTACAA AGATTTTTCC AGTTCATAAG TACATTCTCG CCCTTCGATC TGACTTCTTC 
GGAAAGCTTT TCTCTTCTGG AGAAATCAAT TGCCTTGATT TTCCAGAGGT GCACCAGAAG 
GGGGAGGATG CAGCTGGATG TGATTTGTTT GTCATTGAGA AAATCCCCCC TGAATTGTTT 
GAGTATGTTC TCCAGTTTAT CTATTTTGAC TCTTGCGATA TGCTGTTACA AGGACATAAG 
CCAAAGCTTC GGCATAAAGA AGAAAATGAG GATACAATTC TCCCTACCTT CCAGAAAATG 
GGTTTTCATG AAGAAATTAA GGGCAAGTCT GCCTATGAAG TCTATAAAAA CAGCAGAATC 
AGTGCTGAAA ACGAGAAGCA GAAAGGAAAG ACAAAGCAAT CCAAAAAGAC CAAATCTATT 
TGTGATGAAA CAAGCCCTGT AAAATTGCTG CAAAATACTG CCAAGAAGTT TGGATTGACT 
AACCTAATAT CAAGACTGGA TGGGGTCAGA TATGAAAATG GAAGAATTAA TGTTTTTCAA 
AAGAAAAGTG GAGACAAACT CAGATTCAAC CAAGAGAAAT GCTCTTCCCT TTATGACGTC 
ATGATGAAAT CAGAAGACGG TAAAGAATTC CATTGCCATA AATGTGTTCT TTGCGCAAGA 
TTAGAATATT TTAATAGCAT GCTAAGCAGT TCCTGGATTG AGGCAACTTG TTGTAGTAAG 
CTGCAAATGC CCATCCATTC AGATGTCTTA CAAGTTATTC TTGATTACAT TTATACAGAT 
GAAGCCCTTG CTGTAAAAGA GTCTGCCAAT GTAGAATTTA TTTGTAATGT CCTTGTGATG 
GCAGATCAGC TTCTTATTGT ACGATTAAAA GAAATATGTG AAGTGACAAT AGCTGAAAGA 
ATTACACTGA AGAATGCAGC CGAACTCCTG GAAATTGCAG CCTTGTACAA TGCTGACCAG 
CTGAAGCTTT CTTGCTTGCA GTTTATAGTG CTAAACATGG GAGCTTTACT AGAAGCAAGG 
TCTTTGGATG TTTTGAGTGA TGATGTGTTG AAAGATCTCT CAGAAGCATA TAGAAAAATG 
ATTCCACCCA TGAACAAGAG AATAATCACA CCATATCTGG ATGGGCCAGA TGTTAGTATC 
TTACAGTCTG AAGATATTAA AAGCCTTGTC TCTGTTCAGG ATGATATTTA TTCTTACCAA 
AACACTCAGG AAGCTGCATT TAAAAAATCT AAGGCAAAAG CCAAAAAGAA GCCAAGAAAA 
CGTTTGGATA GCTCTGGAGG ATACAATCTT TCTGACATCA TTCACAGTTC ACCTTCGACA 
GGCTTTGTGA AGCCAGAAAA AACAAATTCA GTGGAGTCTC TTCAAGATTT GCTGACCTCT 
GATTCTGAAG GAAGTTTTGT TGGAGCAAGC AGCCCAAGGG ATTTGCAGTC CCCAGACTTC 
TTCCCTACCT TCACTCCTGA GGCAAAAGAG ACAATTTGTG TTGAAATAAA CGGTTCCTCC 
CCGCCTGTAA CAAACAGAGC AGCAAGCAAA AAGAAGCCCA AACCTACACC TTCATCTCCA 
GAAGTTATCC CAAGTTCTAG AATTACTCCA TCAAGCTCAC CAAACTGGGT CGCCATGCCC 
TTCAGCCCTT CCAGTCCTGT AGCAGTGGAT CTCAGGGCCA TCATGGAACT AGAAGAAAAC 
AAACAGAAGT GTGGAGCAAT GCCAAAGCTT AATTCTGGGG GACCTAAACC TGTCGCTCAT 
GGCATGAAGC TGTCTCAGAA GCAGAGGAAA ATGATGGCAA TGTCCTCTAA GGAAAGCAAT 
AATGACAATA AACCAGCTAT AATAACTGCG ACAGCCTCTA TCAAAAGCCC TGCCAAAACA 
TGGGCAGCAG CTTTTCATTT TGGAGAATGT AAGTCTTTCC GAGAACTTTT GCTTGAAGAA 
AACCAATCTG TCATCTCATT TCCTTCCTTG GGTAGTGATG TCAAAAAGAC CAAGCATACA 
GAGGAATTAG ATCCTCCAGA GCCGGCAAGG CTCTCTGGAT CCATAAGCCA TGAAGCAAAA 
TTGAAATGTG ATGTGCCAAA TCAAGACAGC TCAAATCCTT GGCATTTGAC ATTGAGCAAA 
AATACCTCCT CATCCGCCCC TGTGACTTTT ACTGCCATTG TAGAGGAAGA GGAGAAACAA 
GAAGCTGCTC TCATCAGAAG TCGAGAAAAG CCACTGGCAT TAATTCAGAT AGAAGAACGA 
GCAATCCAAG ATTTGTTAAT ACACTACCAG GCATTAGACA ACCCTGAAGA ATACATTACT 
GTTGAGAGGG CCGCACAGAC ACCTATGGCA ACACCAATGT GGAACAAACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ibtk
Entrez Gene ID	100490098
Full Name	inhibitor of Bruton tyrosine kinase
General protein information	Preferred Names inhibitor of Bruton tyrosine kinase Names inhibitor of Bruton tyrosine kinase inhibitor of Bruton agammaglobulinemia tyrosine kinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	IBTK	XP_002803869.1
Bos taurus (cattle)	IBTK	NP_001192969.1
Drosophila melanogaster (fruit fly)	CG8060	NP_611117.2
Pan troglodytes (chimpanzee)	IBTK	XP_001148806.1
Xenopus tropicalis (tropical clawed frog)	ibtk	XP_002938503.2
Canis lupus familiaris (dog)	IBTK	XP_539018.2
Mus musculus (house mouse)	Ibtk	NP_001074751.1
Rattus norvegicus (Norway rat)	Ibtk	XP_006243569.1
Gallus gallus (chicken)	IBTK	XP_004940475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009939	XP_319065.4
Homo sapiens (human)	IBTK	NP_056340.2
Danio rerio (zebrafish)	ibtk	XP_005157916.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ibtk cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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