LOC100489443 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100489443
Entrez Gene ID	100489443
Full Name	protein unc-13 homolog B-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031894969.1	XP_031750829.1	protein unc-13 homolog B isoform X1
XM_031894971.1	XP_031750831.1	protein unc-13 homolog B isoform X3
XM_031894973.1	XP_031750833.1	protein unc-13 homolog B isoform X5
XM_018090686.1	XP_017946175.1	protein unc-13 homolog B-like
XM_031894975.1	XP_031750835.1	protein unc-13 homolog B isoform X6
XM_031894970.1	XP_031750830.1	protein unc-13 homolog B isoform X2
XM_031894972.1	XP_031750832.1	protein unc-13 homolog B isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC100489443 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100489443 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa41567	XM_018090686.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B-like (LOC100489443), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OXa49304	XM_031894971.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49305	XM_031894972.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49306	XM_031894973.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49307	XM_031894975.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49302	XM_031894969.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa49303	XM_031894970.1 Latest version!	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100489443 ( XM_018090686.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946175.1 Xenopus tropicalis protein unc-13 homolog B-like (LOC100489443), mRNA.

Price & Availability↑

CloneID	OXa41567
Clone ID Related Accession (Same CDS sequence)	XM_018090686.1
Accession Version	XM_018090686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016686561.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGTTCCGAC CCTTCGTGGA GGTGACAATG ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA 
AAGTTCACCA CCAAGTCCAA GAGCAATAAC TGGGCGCCCA AGTACAACGA GACGTTCCAC 
TTCATCCTGG GGAACGAGGA CGGGCCGGAC TGCTACGAGC TGCAGGTGTG CGTGAAGGAT 
TACTGCTTCG CCAGAGAGGA CCGCGTGTTG GGCATCGCCG TCATGCAGCT GCGCGACATT 
GCGGATAAGG GCAGCTGCGC CTGTTGGTGC CCCCTGGGCC GCCGGATACA CATGGACGAC 
ACCGGCCTCA CCGTACTGCG GATCCTCTCC CAGAGAACCA ACGACGAGGT GGCGCGGGAA 
TTCGTCAAGC TGAAGTCGGA GCTGAACTGG GGCAGGAGGA TCCCTTCCCG CCCCATAGTG 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946175.1
CDS	59..484
Translation	MFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSELNWGRRIPSRPIVG

Target ORF information:

RefSeq Version	XM_018090686.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B-like (LOC100489443), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090686.1

ORF Insert Sequence:

ATGTTCCGAC CCTTCGTGGA GGTGACAATG ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA 
AAGTTCACCA CCAAGTCCAA GAGCAATAAC TGGGCGCCCA AGTACAACGA GACGTTCCAC 
TTCATCCTGG GGAACGAGGA CGGGCCGGAC TGCTACGAGC TGCAGGTGTG CGTGAAGGAT 
TACTGCTTCG CCAGAGAGGA CCGCGTGTTG GGCATCGCCG TCATGCAGCT GCGCGACATT 
GCGGATAAGG GCAGCTGCGC CTGTTGGTGC CCCCTGGGCC GCCGGATACA CATGGACGAC 
ACCGGCCTCA CCGTACTGCG GATCCTCTCC CAGAGAACCA ACGACGAGGT GGCGCGGGAA 
TTCGTCAAGC TGAAGTCGGA GCTGAACTGG GGCAGGAGGA TCCCTTCCCG CCCCATAGTG 
GGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894971.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750831.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa49304
Clone ID Related Accession (Same CDS sequence)	XM_031894971.1
Accession Version	XM_031894971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GGCTATGTCG 
CTGGTCCAGT CACGGAAAGC CGGTATCAGC TCCGCCATGG CGACCCGCAC GTCTCTGAAG 
GATGAAGACC TGAAAACCCA CGTGTACAAG AAGACGCTAC AGGCGCTGAT CTACCCCATC 
TCCTGCACAA CCCCCCACAA CTTTGAGGTG TGGACGGCCA CGACCCCCAC CTACTGCTAC 
GAGTGTGAGG GGCTGCTGTG GGGTATTGCC CGCCAGGGGA TGCGCTGCTC CGAGTGTGGC 
GTCAAGTGCC ACGAAAAGTG CCAAGACTTG CTCAATGCCG ACTGCTTGCA GCGGGCGGCG 
GAGAAGAGCT CCAAGCATGG AGCAGAGGAT CGCACCCAGA ATATTATCAT GGCCATGAAA 
GATCGGATGA AGATCCGGGA GAGGAACAAG CCGGAGATAT TTGAGGTGAT CAGGGACGTG 
TTTGATGTGG GGAAGGTGGC CCATGCCCAG CAGATGAAAA CTGTCAAGCA GAGCGTGTTG 
GACGGGACCT CAAAGTGGTC TGCGAAGATC ACGATCACCG TGCTGTGCGC TCAGGGCCTA 
CAGGCCAAGG ATAAGACCGG CTCCTCCGAC CCCTACGTCA CCGTGCAGGT GGGAAAGACA 
AAGAAGAGAA CAAAAACCAT TTACGGGAAC CTGAATCCTG TCTGGGAGGA GAAATTCCAC 
TTCGAGTGCC ATAACTCATC GGATCGCATT AAAGTTCGGG TTTGGGATGA AGATGACGAC 
ATCAAGTCCC GCGTGAAGCA GAGACTGAAG CGCGAATCCG ATGACTTCCT GGGACAGACG 
ATCATTGAAG TTCGAACACT GAGCGGCGAG ATGGACGTGT GGTACAACCT AGAGAAGCGA 
ACAGACAAGT CGGCCGTATC CGGCGCCATT CGGCTGCAGA TCAGCGTGGA GATTAAGGGA 
GAGGAGAAGG TGGCGCCCTA CCATATCCAG TACACCTGCC TGCACGAGAA CCTGTTCCAT 
TACCTGACAG ACGTACAAGG AGGCGGGGTT GTGAAGATCC CAGAAGCCAA AGGGGACGAC 
GCCTGGAAGG TTTACTTCGA TGAGACGGCC CAGGAGATAG TGGACGAGTT TGCAATGCGC 
TACGGCATAG AGTCTATTTA CCAAGCCATG ACACACTTTG CTTGCCTTTC ATCCAAGTAC 
ATGTGCCCGG GGGTCCCGGC CGTGATGAGC ACCTTACTCG CCAATATAAA TGCCTACTAT 
GCACACACCA CGGCGTCCAC CAACGTTTCT GCCTCCGACC GCTTCTCTGC CTCCAACTTT 
GGGAAAGAGA GATTCGTCAA ACTCCTGGAC CAGCTGCACA ACTCCCTGCG CATCGATCTG 
TCTATGTACC GGAACAATTT CCCAGCCAGC AGCCCGGATC GCCTACAGGA TTTGAAATCC 
ACCGTGGACC TACTGACAAG TATCACATTC TTCCGCATGA AGGTGCAAGA GCTGCAGAGC 
CCCCCGCGCG CCAGCCAAGT GGTGAGGGAC TGCGTTAAGG CCTGTCTGAA TTCCACCTAC 
GAGTACATCT TCAACAACTG CCACGAGCTG TACAGCCGCG AGTACCAGGC AGACCCGACT 
AAGAAACAGG ATCTGCCCCC CGAGGAGCAG GGACCGTGCA TAAAGAACTT GGATTTCTGG 
CCCAAACTCA TTACGCTGAT CGTTTCCATC ATTGAGGAAG ATAAGAACGC CTATACCCCT 
GTGCTGAAAC AATTTCCCCA GGAGCTGAGT GTCGGGAAGG TCAGTGCCGA GGTCATGTGG 
AACCTTTTTG CCCAGGACAT GAAATACGCC ATGGAAGAGC ACGAGAAGCA CCGGATGTGC 
AAGAGCGCCG ACTACATGAA CCTTCACTTC AAGGTCAAGT GGTTGTACAA CGAGTATGTG 
CGGGACCTGC CTGCCATGAA GGATAAAGTG CCTGAATACC CAGCGTGGTT TGAGCAGTTT 
GTGATGCAGT GGTTGGATGA GAACGAGGAG GTGTCCATAG AATTCCAGAA CGGCGCCTTG 
GAGCGAGACA AGAAAGATGG GTTCCAGCAG ACGTCGGAAC ACGCCCTGTT CTCTTGCTCG 
GTGGTGGACG TCTTCACGCA ACTCAACCAA AGCTTCGAGA TCATTAAGAA GTTGGAGTGT 
CCGGACCCGG TCATCGTGGC GCATTATATG CGCAGGTTTG CCAAGACCAT CAATAAAGTT 
CTGCTCCAGT ACTCTGAGAT CGTCGCCAAG AGTTTCCAGT CTTACTGCTT CAAGGAGAAA 
CTGCCCTGCA TCCTGATGAA CAACGTGCAG CAACTGAGAG TCCAACTGGA GAAGATGTTT 
GAGGCCATGG GAGGGAAGGA GTTGGATCCC GAGGCCAGCG ACACCCTGAA GGAACTGCAG 
GTCAAACTCA ACACCGTATT GGATGAACTG AGCACTGTGT TTGGGAACAG CTTCCAGTTC 
CGCATAGACG AGTGTATAAA GCAGATGGCA GAGATCCTGG GACACGTGAA GGGCACAGGG 
AATGTGGCAG CCAATGCCAG GAACACGGTG GCCCAGGATG CAGATAATGT ACTGAGGCCC 
CTGATGGATT TCCTGGATGG GAATCTGACC CTGTTTGCCA CGGTGTGTGA GAAGACGGTG 
CTGAAGCGGG TGCTGAAGGA GTTATGGCGA GTAGTAATGA ACACCATGGA GAAGATGATT 
GTGCTCCCGC CCCTTACGGA CCAGTCGGGG ACTCAGATGA TTTTCAGTGC AGCCAAGGAG 
TTGGGCCATT TATCCAAACT GAAGGATCAC ATGGTGAGAG AAGAGACGCG CAGCCTGACC 
CCCAAGCAGT GCGCTGTGAT GGACCTCGCT CTCGATACCA TCAAGCAATA CTTCCACGCC 
GGTGGCAATG GCCTGAAGAA GACCTTTCTG GAGAAAAGTT CAGAACTCCA GTCTCTGCGC 
TACGCCTTGT CCTTGTACAC ACAGACCACA GACACACTCA TCAAAACCTT TGTGCAAACA 
CAGACGTCTC AGGTGCATGA TGGGAAGGGT ATTAGGTATA CGGCTAATGA GGACATTCTT 
CCTGACAAGG GTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750831.1
CDS	482..6079
Translation	MMLARSKLKRQENGTAFRHRKPSVKIFPFKQSKRQKLALCLREKMKRLLQGPVDKISANHDNQIFPVEIDEDLFVCGIKRTPNIAFQKPVDDMELKEEPKELNKTCVEGPDECLTPSLKLPDEQNSCDHDEGNSSNEEDSEVLSPCQSSGLPSDKQDMSAISITMSWREISEHNSLFCPNIPLDPDRESTGSSDRSGDVVEYIIKELQGISRIQAEIAELRQHLSLIKGSVEEVSTCVDSVLNEIEGLQVGVKSPGTASSAELYKDSQNEETVLYFYGVSEHEGENTLDAIHCFLSDHHCFNGVQSKEYIKDAYRLGNGASGLLDPRPTVVRLAHREQRDFILQNSVRLQSAGVRIVPFEEHNIQGLQSRNRANSMSVLQQGLRENRWSSVSLDKGVVRPDDSGGDWKTESFRIRYKNSSTETQCAGEEFVFAQTSTKCNLTAELEATGSQVCQVKENCELEKSVSHLCIRLDSQESQQNLHITNKPSSMDKANCRCARLLTRSSHLSASSEADEAEIAIQRCCSVVDEHTEGLIKGENELVSCDSRRKFRSTEKINVIIKDPSGSFTSLSFDDTVDKRSDGTVAETLKEVVHIDLDTQDQTGHVFRDFLNQPDENIDIVDIKFYANKLGKALNHFRSALKVVFNKLESPEVFMESKESGFPVHDCDSLASVGTGHFNDSIPTQSSRSNSYSSSSNLKEDAYFVLNRVSSLPHETSIPSLILSPDVDLNSAQPLSQSDETMESSELTEELKEAKPMRLDQVCAETIYLNKCINNFKNVLKEKKKMQRKLLKDIVQETFWVSGEEMTSEESRAEYQTQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLAMSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQVHDGKGIRYTANEDILPDKGM

Target ORF information:

RefSeq Version	XM_031894971.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894971.1

ORF Insert Sequence:

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GGCTATGTCG 
CTGGTCCAGT CACGGAAAGC CGGTATCAGC TCCGCCATGG CGACCCGCAC GTCTCTGAAG 
GATGAAGACC TGAAAACCCA CGTGTACAAG AAGACGCTAC AGGCGCTGAT CTACCCCATC 
TCCTGCACAA CCCCCCACAA CTTTGAGGTG TGGACGGCCA CGACCCCCAC CTACTGCTAC 
GAGTGTGAGG GGCTGCTGTG GGGTATTGCC CGCCAGGGGA TGCGCTGCTC CGAGTGTGGC 
GTCAAGTGCC ACGAAAAGTG CCAAGACTTG CTCAATGCCG ACTGCTTGCA GCGGGCGGCG 
GAGAAGAGCT CCAAGCATGG AGCAGAGGAT CGCACCCAGA ATATTATCAT GGCCATGAAA 
GATCGGATGA AGATCCGGGA GAGGAACAAG CCGGAGATAT TTGAGGTGAT CAGGGACGTG 
TTTGATGTGG GGAAGGTGGC CCATGCCCAG CAGATGAAAA CTGTCAAGCA GAGCGTGTTG 
GACGGGACCT CAAAGTGGTC TGCGAAGATC ACGATCACCG TGCTGTGCGC TCAGGGCCTA 
CAGGCCAAGG ATAAGACCGG CTCCTCCGAC CCCTACGTCA CCGTGCAGGT GGGAAAGACA 
AAGAAGAGAA CAAAAACCAT TTACGGGAAC CTGAATCCTG TCTGGGAGGA GAAATTCCAC 
TTCGAGTGCC ATAACTCATC GGATCGCATT AAAGTTCGGG TTTGGGATGA AGATGACGAC 
ATCAAGTCCC GCGTGAAGCA GAGACTGAAG CGCGAATCCG ATGACTTCCT GGGACAGACG 
ATCATTGAAG TTCGAACACT GAGCGGCGAG ATGGACGTGT GGTACAACCT AGAGAAGCGA 
ACAGACAAGT CGGCCGTATC CGGCGCCATT CGGCTGCAGA TCAGCGTGGA GATTAAGGGA 
GAGGAGAAGG TGGCGCCCTA CCATATCCAG TACACCTGCC TGCACGAGAA CCTGTTCCAT 
TACCTGACAG ACGTACAAGG AGGCGGGGTT GTGAAGATCC CAGAAGCCAA AGGGGACGAC 
GCCTGGAAGG TTTACTTCGA TGAGACGGCC CAGGAGATAG TGGACGAGTT TGCAATGCGC 
TACGGCATAG AGTCTATTTA CCAAGCCATG ACACACTTTG CTTGCCTTTC ATCCAAGTAC 
ATGTGCCCGG GGGTCCCGGC CGTGATGAGC ACCTTACTCG CCAATATAAA TGCCTACTAT 
GCACACACCA CGGCGTCCAC CAACGTTTCT GCCTCCGACC GCTTCTCTGC CTCCAACTTT 
GGGAAAGAGA GATTCGTCAA ACTCCTGGAC CAGCTGCACA ACTCCCTGCG CATCGATCTG 
TCTATGTACC GGAACAATTT CCCAGCCAGC AGCCCGGATC GCCTACAGGA TTTGAAATCC 
ACCGTGGACC TACTGACAAG TATCACATTC TTCCGCATGA AGGTGCAAGA GCTGCAGAGC 
CCCCCGCGCG CCAGCCAAGT GGTGAGGGAC TGCGTTAAGG CCTGTCTGAA TTCCACCTAC 
GAGTACATCT TCAACAACTG CCACGAGCTG TACAGCCGCG AGTACCAGGC AGACCCGACT 
AAGAAACAGG ATCTGCCCCC CGAGGAGCAG GGACCGTGCA TAAAGAACTT GGATTTCTGG 
CCCAAACTCA TTACGCTGAT CGTTTCCATC ATTGAGGAAG ATAAGAACGC CTATACCCCT 
GTGCTGAAAC AATTTCCCCA GGAGCTGAGT GTCGGGAAGG TCAGTGCCGA GGTCATGTGG 
AACCTTTTTG CCCAGGACAT GAAATACGCC ATGGAAGAGC ACGAGAAGCA CCGGATGTGC 
AAGAGCGCCG ACTACATGAA CCTTCACTTC AAGGTCAAGT GGTTGTACAA CGAGTATGTG 
CGGGACCTGC CTGCCATGAA GGATAAAGTG CCTGAATACC CAGCGTGGTT TGAGCAGTTT 
GTGATGCAGT GGTTGGATGA GAACGAGGAG GTGTCCATAG AATTCCAGAA CGGCGCCTTG 
GAGCGAGACA AGAAAGATGG GTTCCAGCAG ACGTCGGAAC ACGCCCTGTT CTCTTGCTCG 
GTGGTGGACG TCTTCACGCA ACTCAACCAA AGCTTCGAGA TCATTAAGAA GTTGGAGTGT 
CCGGACCCGG TCATCGTGGC GCATTATATG CGCAGGTTTG CCAAGACCAT CAATAAAGTT 
CTGCTCCAGT ACTCTGAGAT CGTCGCCAAG AGTTTCCAGT CTTACTGCTT CAAGGAGAAA 
CTGCCCTGCA TCCTGATGAA CAACGTGCAG CAACTGAGAG TCCAACTGGA GAAGATGTTT 
GAGGCCATGG GAGGGAAGGA GTTGGATCCC GAGGCCAGCG ACACCCTGAA GGAACTGCAG 
GTCAAACTCA ACACCGTATT GGATGAACTG AGCACTGTGT TTGGGAACAG CTTCCAGTTC 
CGCATAGACG AGTGTATAAA GCAGATGGCA GAGATCCTGG GACACGTGAA GGGCACAGGG 
AATGTGGCAG CCAATGCCAG GAACACGGTG GCCCAGGATG CAGATAATGT ACTGAGGCCC 
CTGATGGATT TCCTGGATGG GAATCTGACC CTGTTTGCCA CGGTGTGTGA GAAGACGGTG 
CTGAAGCGGG TGCTGAAGGA GTTATGGCGA GTAGTAATGA ACACCATGGA GAAGATGATT 
GTGCTCCCGC CCCTTACGGA CCAGTCGGGG ACTCAGATGA TTTTCAGTGC AGCCAAGGAG 
TTGGGCCATT TATCCAAACT GAAGGATCAC ATGGTGAGAG AAGAGACGCG CAGCCTGACC 
CCCAAGCAGT GCGCTGTGAT GGACCTCGCT CTCGATACCA TCAAGCAATA CTTCCACGCC 
GGTGGCAATG GCCTGAAGAA GACCTTTCTG GAGAAAAGTT CAGAACTCCA GTCTCTGCGC 
TACGCCTTGT CCTTGTACAC ACAGACCACA GACACACTCA TCAAAACCTT TGTGCAAACA 
CAGACGTCTC AGGTGCATGA TGGGAAGGGT ATTAGGTATA CGGCTAATGA GGACATTCTT 
CCTGACAAGG GTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894972.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750832.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X4, mRNA.

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CloneID	OXa49305
Clone ID Related Accession (Same CDS sequence)	XM_031894972.1
Accession Version	XM_031894972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGTCCG GCCAGCAGTA GCCGATATGG CTCTTCGGGC 
AATGTCAGCC AAGGAAGTTC ACAACTCAGT GACTCGGACC ATTACCACGA AAACCTTCAG 
TGCCTGGAGC TGGAAGATGA ACCCAGGGAA TCAAATTATT CCTATCACCC GGCGGCCAAT 
GTGAGGAAGA ATGGGCCAGA ATGGTACAGT GACCACGAGG CAGAAAGAGC GGCCAATGAG 
GTTATACTGG AGAAAGTCCC AACCCAAGTC ACTCCAGAAC CTGATCGGCC CGAAGACATC 
GTCATAGAGA AAAGAGAGAG CTTCCATGAA GACATTGGGC CGCCACCGTT TTCCCCTGCA 
AGAGCTCATT GGCTCCGAGC TATCAACAAA GTGCGAACCC AGATCCAAGA GACACAAGAT 
GATGGGGATC CGTCTAAGCC ACCGTGGTTT AAAGGAGGAC CGGCGGGAGG GCTCTATGGC 
ATCGACAGTA TGCCCGATCT GCGCAAGAAG AAGCCCATCC CTCTGGTCAG CGACTTGGCT 
ATGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGG TTCTGGTGTG GAGGATCCAG TGGGAGAAGT TTCCATACAG 
GTTGACTTGT TCACACACCC AGGGACTGGG GAGCAGAAGG TGACAGTGAA AGTGGTGGCG 
GCAAATGATC TAAAATGGCA GACGACGGGA ATGTTCCGAC CCTTCGTGGA GGTGACAATG 
ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA AAGTTCACCA CCAAGTCCAA GAGCAATAAC 
TGGGCGCCCA AGTACAACGA GACGTTCCAC TTCATCCTGG GGAACGAGGA CGGGCCGGAC 
TGCTACGAGC TGCAGGTGTG CGTGAAGGAT TACTGCTTCG CCAGAGAGGA CCGCGTGTTG 
GGCATCGCCG TCATGCAGCT GCGCGACATT GCGGATAAGG GCAGCTGCGC CTGTTGGTGC 
CCCCTGGGGC GCCGGATACA CATGGACGAC ACCGGCCTCA CCGTACTGCG GATCCTCTCC 
CAGAGAACCA ACGACGAGGT GGCGCGGGAA TTCGTCAAGC TGAAGTCGGA CACTCGCTCC 
ACGGAGGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750832.1
CDS	557..5314
Translation	MSLLCVRVKKAKLQGPPDKFNTYVTLKVQNVKSTTVAVRGDQPCWEQDFMFEISRLDLGLVVEMWNKGLIWDTMVGTVWIPLQTVRQSEEEGPGEWAALESEVLMKNDEICGTQNPTLHRILLDTRFELPFDIPEEEARYWTRKLIPEDTSEEPEEYPYDDVDHKKPLPIAASQCSLEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASIHQFPVQSRIQQQGGTRESYVDSMHGYDLDFRERTMSPASSSRYGSSGNVSQGSSQLSDSDHYHENLQCLELEDEPRESNYSYHPAANVRKNGPEWYSDHEAERAANEVILEKVPTQVTPEPDRPEDIVIEKRESFHEDIGPPPFSPARAHWLRAINKVRTQIQETQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLAMSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQGSGVEDPVGEVSIQVDLFTHPGTGEQKVTVKVVAANDLKWQTTGMFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSDTRSTEEGS

Target ORF information:

RefSeq Version	XM_031894972.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894972.1

ORF Insert Sequence:

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGTCCG GCCAGCAGTA GCCGATATGG CTCTTCGGGC 
AATGTCAGCC AAGGAAGTTC ACAACTCAGT GACTCGGACC ATTACCACGA AAACCTTCAG 
TGCCTGGAGC TGGAAGATGA ACCCAGGGAA TCAAATTATT CCTATCACCC GGCGGCCAAT 
GTGAGGAAGA ATGGGCCAGA ATGGTACAGT GACCACGAGG CAGAAAGAGC GGCCAATGAG 
GTTATACTGG AGAAAGTCCC AACCCAAGTC ACTCCAGAAC CTGATCGGCC CGAAGACATC 
GTCATAGAGA AAAGAGAGAG CTTCCATGAA GACATTGGGC CGCCACCGTT TTCCCCTGCA 
AGAGCTCATT GGCTCCGAGC TATCAACAAA GTGCGAACCC AGATCCAAGA GACACAAGAT 
GATGGGGATC CGTCTAAGCC ACCGTGGTTT AAAGGAGGAC CGGCGGGAGG GCTCTATGGC 
ATCGACAGTA TGCCCGATCT GCGCAAGAAG AAGCCCATCC CTCTGGTCAG CGACTTGGCT 
ATGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGG TTCTGGTGTG GAGGATCCAG TGGGAGAAGT TTCCATACAG 
GTTGACTTGT TCACACACCC AGGGACTGGG GAGCAGAAGG TGACAGTGAA AGTGGTGGCG 
GCAAATGATC TAAAATGGCA GACGACGGGA ATGTTCCGAC CCTTCGTGGA GGTGACAATG 
ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA AAGTTCACCA CCAAGTCCAA GAGCAATAAC 
TGGGCGCCCA AGTACAACGA GACGTTCCAC TTCATCCTGG GGAACGAGGA CGGGCCGGAC 
TGCTACGAGC TGCAGGTGTG CGTGAAGGAT TACTGCTTCG CCAGAGAGGA CCGCGTGTTG 
GGCATCGCCG TCATGCAGCT GCGCGACATT GCGGATAAGG GCAGCTGCGC CTGTTGGTGC 
CCCCTGGGGC GCCGGATACA CATGGACGAC ACCGGCCTCA CCGTACTGCG GATCCTCTCC 
CAGAGAACCA ACGACGAGGT GGCGCGGGAA TTCGTCAAGC TGAAGTCGGA CACTCGCTCC 
ACGGAGGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894973.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750833.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X5, mRNA.

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CloneID	OXa49306
Clone ID Related Accession (Same CDS sequence)	XM_031894973.1
Accession Version	XM_031894973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGGAGG TATGATAGCA TAGATCCTTC TGCGAATGGA 
CGCAACGATC TAGAGTCTGC ATCTGAATCT AGTCAGCTGA CAAGCCGCCA TTACTCGCTA 
GAAAGCAGGC ATTCTCTAGA TCTGTCCGAT AGTCCGGCCA GCAGTAGCCG ATATGGCTCT 
TCGGGCAATG TCAGCCAAGG AAGTTCACAA CTCAGTGACT CGGACCATTA CCACGAAAAC 
CTTCAGTGCC TGGAGCTGGA AGATGAACCC AGGGAATCAA ATTATTCCTA TCACCCGGCG 
GCCAATGTGA GGAAGAATGG GCCAGAATGG TACAGTGACC ACGAGGCAGA AAGAGCGGCC 
AATGAGGTTA TACTGGAGAA AGTCCCAACC CAAGTCACTC CAGAACCTGA TCGGCCCGAA 
GACATCGTCA TAGAGAAAAG AGAGAGCTTC CATGAAGACA TTGGGCCGCC ACCGTTTTCC 
CCTGCAAGAG CTCATTGGCT CCGAGCTATC AACAAAGTGC GAACCCAGAT CCAAGAGACA 
CAAGATGATG GGGATCCGTC TAAGCCACCG TGGTTTAAAG GAGGACCGGC GGGAGGGCTC 
TATGGCATCG ACAGTATGCC CGATCTGCGC AAGAAGAAGC CCATCCCTCT GGTCAGCGAC 
TTGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGG TTCTGGTGTG GAGGATCCAG TGGGAGAAGT TTCCATACAG 
GTTGACTTGT TCACACACCC AGGGACTGGG GAGCAGAAGG TGACAGTGAA AGTGGTGGCG 
GCAAATGATC TAAAATGGCA GACGACGGGA ATGTTCCGAC CCTTCGTGGA GGTGACAATG 
ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA AAGTTCACCA CCAAGTCCAA GAGCAATAAC 
TGGGCGCCCA AGTACAACGA GACGTTCCAC TTCATCCTGG GGAACGAGGA CGGGCCGGAC 
TGCTACGAGC TGCAGGTGTG CGTGAAGGAT TACTGCTTCG CCAGAGAGGA CCGCGTGTTG 
GGCATCGCCG TCATGCAGCT GCGCGACATT GCGGATAAGG GCAGCTGCGC CTGTTGGTGC 
CCCCTGGGGC GCCGGATACA CATGGACGAC ACCGGCCTCA CCGTACTGCG GATCCTCTCC 
CAGAGAACCA ACGACGAGGT GGCGCGGGAA TTCGTCAAGC TGAAGTCGGA CACTCGCTCC 
ACGGAGGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750833.1
CDS	557..5434
Translation	MSLLCVRVKKAKLQGPPDKFNTYVTLKVQNVKSTTVAVRGDQPCWEQDFMFEISRLDLGLVVEMWNKGLIWDTMVGTVWIPLQTVRQSEEEGPGEWAALESEVLMKNDEICGTQNPTLHRILLDTRFELPFDIPEEEARYWTRKLIPEDTSEEPEEYPYDDVDHKKPLPIAASQCSLEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASIHQFPVQSRIQQQGGTRESYVDSMHGYDLDFRERTMRRYDSIDPSANGRNDLESASESSQLTSRHYSLESRHSLDLSDSPASSSRYGSSGNVSQGSSQLSDSDHYHENLQCLELEDEPRESNYSYHPAANVRKNGPEWYSDHEAERAANEVILEKVPTQVTPEPDRPEDIVIEKRESFHEDIGPPPFSPARAHWLRAINKVRTQIQETQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQGSGVEDPVGEVSIQVDLFTHPGTGEQKVTVKVVAANDLKWQTTGMFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSDTRSTEEGS

Target ORF information:

RefSeq Version	XM_031894973.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894973.1

ORF Insert Sequence:

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGGAGG TATGATAGCA TAGATCCTTC TGCGAATGGA 
CGCAACGATC TAGAGTCTGC ATCTGAATCT AGTCAGCTGA CAAGCCGCCA TTACTCGCTA 
GAAAGCAGGC ATTCTCTAGA TCTGTCCGAT AGTCCGGCCA GCAGTAGCCG ATATGGCTCT 
TCGGGCAATG TCAGCCAAGG AAGTTCACAA CTCAGTGACT CGGACCATTA CCACGAAAAC 
CTTCAGTGCC TGGAGCTGGA AGATGAACCC AGGGAATCAA ATTATTCCTA TCACCCGGCG 
GCCAATGTGA GGAAGAATGG GCCAGAATGG TACAGTGACC ACGAGGCAGA AAGAGCGGCC 
AATGAGGTTA TACTGGAGAA AGTCCCAACC CAAGTCACTC CAGAACCTGA TCGGCCCGAA 
GACATCGTCA TAGAGAAAAG AGAGAGCTTC CATGAAGACA TTGGGCCGCC ACCGTTTTCC 
CCTGCAAGAG CTCATTGGCT CCGAGCTATC AACAAAGTGC GAACCCAGAT CCAAGAGACA 
CAAGATGATG GGGATCCGTC TAAGCCACCG TGGTTTAAAG GAGGACCGGC GGGAGGGCTC 
TATGGCATCG ACAGTATGCC CGATCTGCGC AAGAAGAAGC CCATCCCTCT GGTCAGCGAC 
TTGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGG TTCTGGTGTG GAGGATCCAG TGGGAGAAGT TTCCATACAG 
GTTGACTTGT TCACACACCC AGGGACTGGG GAGCAGAAGG TGACAGTGAA AGTGGTGGCG 
GCAAATGATC TAAAATGGCA GACGACGGGA ATGTTCCGAC CCTTCGTGGA GGTGACAATG 
ATCGGCCCCC ACCAGAGCGA CAAGAAGAGA AAGTTCACCA CCAAGTCCAA GAGCAATAAC 
TGGGCGCCCA AGTACAACGA GACGTTCCAC TTCATCCTGG GGAACGAGGA CGGGCCGGAC 
TGCTACGAGC TGCAGGTGTG CGTGAAGGAT TACTGCTTCG CCAGAGAGGA CCGCGTGTTG 
GGCATCGCCG TCATGCAGCT GCGCGACATT GCGGATAAGG GCAGCTGCGC CTGTTGGTGC 
CCCCTGGGGC GCCGGATACA CATGGACGAC ACCGGCCTCA CCGTACTGCG GATCCTCTCC 
CAGAGAACCA ACGACGAGGT GGCGCGGGAA TTCGTCAAGC TGAAGTCGGA CACTCGCTCC 
ACGGAGGAGG GGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894975.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750835.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa49307
Clone ID Related Accession (Same CDS sequence)	XM_031894975.1
Accession Version	XM_031894975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGGAGG TATGATAGCA TAGATCCTTC TGCGAATGGA 
CGCAACGATC TAGAGTCTGC ATCTGAATCT AGTCAGCTGA CAAGCCGCCA TTACTCGCTA 
GAAAGCAGGC ATTCTCTAGA TCTGTCCGAT AGTCCGGCCA GCAGTAGCCG ATATGGCTCT 
TCGGGCAATG TCAGCCAAGG AAGTTCACAA CTCAGTGACT CGGACCATTA CCACGAAAAC 
CTTCAGTGCC TGGAGCTGGA AGATGAACCC AGGGAATCAA ATTATTCCTA TCACCCGGCG 
GCCAATGTGA GGAAGAATGG GCCAGAATGG TACAGTGACC ACGAGGCAGA AAGAGCGGCC 
AATGAGGTTA TACTGGAGAA AGTCCCAACC CAAGTCACTC CAGAACCTGA TCGGCCCGAA 
GACATCGTCA TAGAGAAAAG AGAGAGCTTC CATGAAGACA TTGGGCCGCC ACCGTTTTCC 
CCTGCAAGAG CTCATTGGCT CCGAGCTATC AACAAAGTGC GAACCCAGAT CCAAGAGACA 
CAAGATGATG GGGATCCGTC TAAGCCACCG TGGTTTAAAG GAGGACCGGC GGGAGGGCTC 
TATGGCATCG ACAGTATGCC CGATCTGCGC AAGAAGAAGC CCATCCCTCT GGTCAGCGAC 
TTGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGT GCATGATGGG AAGGGTATTA GGTATACGGC TAATGAGGAC 
ATTCTTCCTG ACAAGGGTTC TGGTGTGGAG GATCCAGTGG GAGAAGTTTC CATACAGGTT 
GACTTGTTCA CACACCCAGG GACTGGGGAG CAGAAGGTGA CAGTGAAAGT GGTGGCGGCA 
AATGATCTAA AATGGCAGAC GACGGGAATG TTCCGACCCT TCGTGGAGGT GACAATGATC 
GGCCCCCACC AGAGCGACAA GAAGAGAAAG TTCACCACCA AGTCCAAGAG CAATAACTGG 
GCGCCCAAGT ACAACGAGAC GTTCCACTTC ATCCTGGGGA ACGAGGACGG GCCGGACTGC 
TACGAGCTGC AGGTGTGCGT GAAGGATTAC TGCTTCGCCA GAGAGGACCG CGTGTTGGGC 
ATCGCCGTCA TGCAGCTGCG CGACATTGCG GATAAGGGCA GCTGCGCCTG TTGGTGCCCC 
CTGGGGCGCC GGATACACAT GGACGACACC GGCCTCACCG TACTGCGGAT CCTCTCCCAG 
AGAACCAACG ACGAGGTGGC GCGGGAATTC GTCAAGCTGA AGTCGGACAC TCGCTCCACG 
GAGGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750835.1
CDS	558..5492
Translation	MSLLCVRVKKAKLQGPPDKFNTYVTLKVQNVKSTTVAVRGDQPCWEQDFMFEISRLDLGLVVEMWNKGLIWDTMVGTVWIPLQTVRQSEEEGPGEWAALESEVLMKNDEICGTQNPTLHRILLDTRFELPFDIPEEEARYWTRKLIPEDTSEEPEEYPYDDVDHKKPLPIAASQCSLEDPDSAVDDRDSDYRSETSNSIPPPYHTTSQPNASIHQFPVQSRIQQQGGTRESYVDSMHGYDLDFRERTMRRYDSIDPSANGRNDLESASESSQLTSRHYSLESRHSLDLSDSPASSSRYGSSGNVSQGSSQLSDSDHYHENLQCLELEDEPRESNYSYHPAANVRKNGPEWYSDHEAERAANEVILEKVPTQVTPEPDRPEDIVIEKRESFHEDIGPPPFSPARAHWLRAINKVRTQIQETQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQVHDGKGIRYTANEDILPDKGSGVEDPVGEVSIQVDLFTHPGTGEQKVTVKVVAANDLKWQTTGMFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSDTRSTEEGS

Target ORF information:

RefSeq Version	XM_031894975.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894975.1

ORF Insert Sequence:

ATGTCGCTCC TGTGTGTGCG AGTTAAAAAG GCCAAACTTC AAGGACCCCC AGATAAGTTT 
AACACTTACG TGACCTTGAA AGTGCAAAAT GTGAAGAGCA CGACGGTGGC GGTGCGGGGG 
GACCAACCCT GCTGGGAGCA AGACTTCATG TTTGAGATCA GTCGGCTGGA CCTGGGGCTG 
GTGGTGGAGA TGTGGAATAA GGGGCTCATC TGGGACACAA TGGTGGGAAC CGTGTGGATT 
CCATTACAGA CAGTCAGGCA GTCGGAAGAG GAGGGGCCTG GGGAATGGGC TGCCCTAGAA 
TCTGAAGTCT TAATGAAGAA CGATGAGATC TGTGGTACCC AGAACCCAAC GCTCCATAGG 
ATTCTACTGG ATACACGATT CGAATTGCCT TTTGATATCC CAGAGGAAGA GGCCCGGTAC 
TGGACTAGGA AGCTGATACC AGAGGACACC TCAGAGGAGC CGGAGGAGTA TCCATACGAT 
GATGTCGACC ACAAGAAGCC GCTGCCCATC GCTGCGTCCC AGTGCTCCCT TGAAGACCCT 
GACAGCGCAG TGGATGACCG GGACAGCGAT TACCGCAGTG AAACGAGCAA CAGTATTCCA 
CCTCCCTATC ACACCACCTC CCAACCCAAT GCCTCCATAC ACCAGTTCCC CGTGCAGTCT 
CGCATCCAGC AGCAGGGCGG CACTCGGGAA TCCTACGTAG ACTCCATGCA CGGCTATGAT 
CTGGATTTCC GGGAAAGAAC AATGAGGAGG TATGATAGCA TAGATCCTTC TGCGAATGGA 
CGCAACGATC TAGAGTCTGC ATCTGAATCT AGTCAGCTGA CAAGCCGCCA TTACTCGCTA 
GAAAGCAGGC ATTCTCTAGA TCTGTCCGAT AGTCCGGCCA GCAGTAGCCG ATATGGCTCT 
TCGGGCAATG TCAGCCAAGG AAGTTCACAA CTCAGTGACT CGGACCATTA CCACGAAAAC 
CTTCAGTGCC TGGAGCTGGA AGATGAACCC AGGGAATCAA ATTATTCCTA TCACCCGGCG 
GCCAATGTGA GGAAGAATGG GCCAGAATGG TACAGTGACC ACGAGGCAGA AAGAGCGGCC 
AATGAGGTTA TACTGGAGAA AGTCCCAACC CAAGTCACTC CAGAACCTGA TCGGCCCGAA 
GACATCGTCA TAGAGAAAAG AGAGAGCTTC CATGAAGACA TTGGGCCGCC ACCGTTTTCC 
CCTGCAAGAG CTCATTGGCT CCGAGCTATC AACAAAGTGC GAACCCAGAT CCAAGAGACA 
CAAGATGATG GGGATCCGTC TAAGCCACCG TGGTTTAAAG GAGGACCGGC GGGAGGGCTC 
TATGGCATCG ACAGTATGCC CGATCTGCGC AAGAAGAAGC CCATCCCTCT GGTCAGCGAC 
TTGTCGCTGG TCCAGTCACG GAAAGCCGGT ATCAGCTCCG CCATGGCGAC CCGCACGTCT 
CTGAAGGATG AAGACCTGAA AACCCACGTG TACAAGAAGA CGCTACAGGC GCTGATCTAC 
CCCATCTCCT GCACAACCCC CCACAACTTT GAGGTGTGGA CGGCCACGAC CCCCACCTAC 
TGCTACGAGT GTGAGGGGCT GCTGTGGGGT ATTGCCCGCC AGGGGATGCG CTGCTCCGAG 
TGTGGCGTCA AGTGCCACGA AAAGTGCCAA GACTTGCTCA ATGCCGACTG CTTGCAGCGG 
GCGGCGGAGA AGAGCTCCAA GCATGGAGCA GAGGATCGCA CCCAGAATAT TATCATGGCC 
ATGAAAGATC GGATGAAGAT CCGGGAGAGG AACAAGCCGG AGATATTTGA GGTGATCAGG 
GACGTGTTTG ATGTGGGGAA GGTGGCCCAT GCCCAGCAGA TGAAAACTGT CAAGCAGAGC 
GTGTTGGACG GGACCTCAAA GTGGTCTGCG AAGATCACGA TCACCGTGCT GTGCGCTCAG 
GGCCTACAGG CCAAGGATAA GACCGGCTCC TCCGACCCCT ACGTCACCGT GCAGGTGGGA 
AAGACAAAGA AGAGAACAAA AACCATTTAC GGGAACCTGA ATCCTGTCTG GGAGGAGAAA 
TTCCACTTCG AGTGCCATAA CTCATCGGAT CGCATTAAAG TTCGGGTTTG GGATGAAGAT 
GACGACATCA AGTCCCGCGT GAAGCAGAGA CTGAAGCGCG AATCCGATGA CTTCCTGGGA 
CAGACGATCA TTGAAGTTCG AACACTGAGC GGCGAGATGG ACGTGTGGTA CAACCTAGAG 
AAGCGAACAG ACAAGTCGGC CGTATCCGGC GCCATTCGGC TGCAGATCAG CGTGGAGATT 
AAGGGAGAGG AGAAGGTGGC GCCCTACCAT ATCCAGTACA CCTGCCTGCA CGAGAACCTG 
TTCCATTACC TGACAGACGT ACAAGGAGGC GGGGTTGTGA AGATCCCAGA AGCCAAAGGG 
GACGACGCCT GGAAGGTTTA CTTCGATGAG ACGGCCCAGG AGATAGTGGA CGAGTTTGCA 
ATGCGCTACG GCATAGAGTC TATTTACCAA GCCATGACAC ACTTTGCTTG CCTTTCATCC 
AAGTACATGT GCCCGGGGGT CCCGGCCGTG ATGAGCACCT TACTCGCCAA TATAAATGCC 
TACTATGCAC ACACCACGGC GTCCACCAAC GTTTCTGCCT CCGACCGCTT CTCTGCCTCC 
AACTTTGGGA AAGAGAGATT CGTCAAACTC CTGGACCAGC TGCACAACTC CCTGCGCATC 
GATCTGTCTA TGTACCGGAA CAATTTCCCA GCCAGCAGCC CGGATCGCCT ACAGGATTTG 
AAATCCACCG TGGACCTACT GACAAGTATC ACATTCTTCC GCATGAAGGT GCAAGAGCTG 
CAGAGCCCCC CGCGCGCCAG CCAAGTGGTG AGGGACTGCG TTAAGGCCTG TCTGAATTCC 
ACCTACGAGT ACATCTTCAA CAACTGCCAC GAGCTGTACA GCCGCGAGTA CCAGGCAGAC 
CCGACTAAGA AACAGGATCT GCCCCCCGAG GAGCAGGGAC CGTGCATAAA GAACTTGGAT 
TTCTGGCCCA AACTCATTAC GCTGATCGTT TCCATCATTG AGGAAGATAA GAACGCCTAT 
ACCCCTGTGC TGAAACAATT TCCCCAGGAG CTGAGTGTCG GGAAGGTCAG TGCCGAGGTC 
ATGTGGAACC TTTTTGCCCA GGACATGAAA TACGCCATGG AAGAGCACGA GAAGCACCGG 
ATGTGCAAGA GCGCCGACTA CATGAACCTT CACTTCAAGG TCAAGTGGTT GTACAACGAG 
TATGTGCGGG ACCTGCCTGC CATGAAGGAT AAAGTGCCTG AATACCCAGC GTGGTTTGAG 
CAGTTTGTGA TGCAGTGGTT GGATGAGAAC GAGGAGGTGT CCATAGAATT CCAGAACGGC 
GCCTTGGAGC GAGACAAGAA AGATGGGTTC CAGCAGACGT CGGAACACGC CCTGTTCTCT 
TGCTCGGTGG TGGACGTCTT CACGCAACTC AACCAAAGCT TCGAGATCAT TAAGAAGTTG 
GAGTGTCCGG ACCCGGTCAT CGTGGCGCAT TATATGCGCA GGTTTGCCAA GACCATCAAT 
AAAGTTCTGC TCCAGTACTC TGAGATCGTC GCCAAGAGTT TCCAGTCTTA CTGCTTCAAG 
GAGAAACTGC CCTGCATCCT GATGAACAAC GTGCAGCAAC TGAGAGTCCA ACTGGAGAAG 
ATGTTTGAGG CCATGGGAGG GAAGGAGTTG GATCCCGAGG CCAGCGACAC CCTGAAGGAA 
CTGCAGGTCA AACTCAACAC CGTATTGGAT GAACTGAGCA CTGTGTTTGG GAACAGCTTC 
CAGTTCCGCA TAGACGAGTG TATAAAGCAG ATGGCAGAGA TCCTGGGACA CGTGAAGGGC 
ACAGGGAATG TGGCAGCCAA TGCCAGGAAC ACGGTGGCCC AGGATGCAGA TAATGTACTG 
AGGCCCCTGA TGGATTTCCT GGATGGGAAT CTGACCCTGT TTGCCACGGT GTGTGAGAAG 
ACGGTGCTGA AGCGGGTGCT GAAGGAGTTA TGGCGAGTAG TAATGAACAC CATGGAGAAG 
ATGATTGTGC TCCCGCCCCT TACGGACCAG TCGGGGACTC AGATGATTTT CAGTGCAGCC 
AAGGAGTTGG GCCATTTATC CAAACTGAAG GATCACATGG TGAGAGAAGA GACGCGCAGC 
CTGACCCCCA AGCAGTGCGC TGTGATGGAC CTCGCTCTCG ATACCATCAA GCAATACTTC 
CACGCCGGTG GCAATGGCCT GAAGAAGACC TTTCTGGAGA AAAGTTCAGA ACTCCAGTCT 
CTGCGCTACG CCTTGTCCTT GTACACACAG ACCACAGACA CACTCATCAA AACCTTTGTG 
CAAACACAGA CGTCTCAGGT GCATGATGGG AAGGGTATTA GGTATACGGC TAATGAGGAC 
ATTCTTCCTG ACAAGGGTTC TGGTGTGGAG GATCCAGTGG GAGAAGTTTC CATACAGGTT 
GACTTGTTCA CACACCCAGG GACTGGGGAG CAGAAGGTGA CAGTGAAAGT GGTGGCGGCA 
AATGATCTAA AATGGCAGAC GACGGGAATG TTCCGACCCT TCGTGGAGGT GACAATGATC 
GGCCCCCACC AGAGCGACAA GAAGAGAAAG TTCACCACCA AGTCCAAGAG CAATAACTGG 
GCGCCCAAGT ACAACGAGAC GTTCCACTTC ATCCTGGGGA ACGAGGACGG GCCGGACTGC 
TACGAGCTGC AGGTGTGCGT GAAGGATTAC TGCTTCGCCA GAGAGGACCG CGTGTTGGGC 
ATCGCCGTCA TGCAGCTGCG CGACATTGCG GATAAGGGCA GCTGCGCCTG TTGGTGCCCC 
CTGGGGCGCC GGATACACAT GGACGACACC GGCCTCACCG TACTGCGGAT CCTCTCCCAG 
AGAACCAACG ACGAGGTGGC GCGGGAATTC GTCAAGCTGA AGTCGGACAC TCGCTCCACG 
GAGGAGGGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894969.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750829.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X1, mRNA.

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CloneID	OXa49302
Clone ID Related Accession (Same CDS sequence)	XM_031894969.1
Accession Version	XM_031894969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GGCTATGTCG 
CTGGTCCAGT CACGGAAAGC CGGTATCAGC TCCGCCATGG CGACCCGCAC GTCTCTGAAG 
GATGAAGACC TGAAAACCCA CGTGTACAAG AAGACGCTAC AGGCGCTGAT CTACCCCATC 
TCCTGCACAA CCCCCCACAA CTTTGAGGTG TGGACGGCCA CGACCCCCAC CTACTGCTAC 
GAGTGTGAGG GGCTGCTGTG GGGTATTGCC CGCCAGGGGA TGCGCTGCTC CGAGTGTGGC 
GTCAAGTGCC ACGAAAAGTG CCAAGACTTG CTCAATGCCG ACTGCTTGCA GCGGGCGGCG 
GAGAAGAGCT CCAAGCATGG AGCAGAGGAT CGCACCCAGA ATATTATCAT GGCCATGAAA 
GATCGGATGA AGATCCGGGA GAGGAACAAG CCGGAGATAT TTGAGGTGAT CAGGGACGTG 
TTTGATGTGG GGAAGGTGGC CCATGCCCAG CAGATGAAAA CTGTCAAGCA GAGCGTGTTG 
GACGGGACCT CAAAGTGGTC TGCGAAGATC ACGATCACCG TGCTGTGCGC TCAGGGCCTA 
CAGGCCAAGG ATAAGACCGG CTCCTCCGAC CCCTACGTCA CCGTGCAGGT GGGAAAGACA 
AAGAAGAGAA CAAAAACCAT TTACGGGAAC CTGAATCCTG TCTGGGAGGA GAAATTCCAC 
TTCGAGTGCC ATAACTCATC GGATCGCATT AAAGTTCGGG TTTGGGATGA AGATGACGAC 
ATCAAGTCCC GCGTGAAGCA GAGACTGAAG CGCGAATCCG ATGACTTCCT GGGACAGACG 
ATCATTGAAG TTCGAACACT GAGCGGCGAG ATGGACGTGT GGTACAACCT AGAGAAGCGA 
ACAGACAAGT CGGCCGTATC CGGCGCCATT CGGCTGCAGA TCAGCGTGGA GATTAAGGGA 
GAGGAGAAGG TGGCGCCCTA CCATATCCAG TACACCTGCC TGCACGAGAA CCTGTTCCAT 
TACCTGACAG ACGTACAAGG AGGCGGGGTT GTGAAGATCC CAGAAGCCAA AGGGGACGAC 
GCCTGGAAGG TTTACTTCGA TGAGACGGCC CAGGAGATAG TGGACGAGTT TGCAATGCGC 
TACGGCATAG AGTCTATTTA CCAAGCCATG ACACACTTTG CTTGCCTTTC ATCCAAGTAC 
ATGTGCCCGG GGGTCCCGGC CGTGATGAGC ACCTTACTCG CCAATATAAA TGCCTACTAT 
GCACACACCA CGGCGTCCAC CAACGTTTCT GCCTCCGACC GCTTCTCTGC CTCCAACTTT 
GGGAAAGAGA GATTCGTCAA ACTCCTGGAC CAGCTGCACA ACTCCCTGCG CATCGATCTG 
TCTATGTACC GGAACAATTT CCCAGCCAGC AGCCCGGATC GCCTACAGGA TTTGAAATCC 
ACCGTGGACC TACTGACAAG TATCACATTC TTCCGCATGA AGGTGCAAGA GCTGCAGAGC 
CCCCCGCGCG CCAGCCAAGT GGTGAGGGAC TGCGTTAAGG CCTGTCTGAA TTCCACCTAC 
GAGTACATCT TCAACAACTG CCACGAGCTG TACAGCCGCG AGTACCAGGC AGACCCGACT 
AAGAAACAGG ATCTGCCCCC CGAGGAGCAG GGACCGTGCA TAAAGAACTT GGATTTCTGG 
CCCAAACTCA TTACGCTGAT CGTTTCCATC ATTGAGGAAG ATAAGAACGC CTATACCCCT 
GTGCTGAAAC AATTTCCCCA GGAGCTGAGT GTCGGGAAGG TCAGTGCCGA GGTCATGTGG 
AACCTTTTTG CCCAGGACAT GAAATACGCC ATGGAAGAGC ACGAGAAGCA CCGGATGTGC 
AAGAGCGCCG ACTACATGAA CCTTCACTTC AAGGTCAAGT GGTTGTACAA CGAGTATGTG 
CGGGACCTGC CTGCCATGAA GGATAAAGTG CCTGAATACC CAGCGTGGTT TGAGCAGTTT 
GTGATGCAGT GGTTGGATGA GAACGAGGAG GTGTCCATAG AATTCCAGAA CGGCGCCTTG 
GAGCGAGACA AGAAAGATGG GTTCCAGCAG ACGTCGGAAC ACGCCCTGTT CTCTTGCTCG 
GTGGTGGACG TCTTCACGCA ACTCAACCAA AGCTTCGAGA TCATTAAGAA GTTGGAGTGT 
CCGGACCCGG TCATCGTGGC GCATTATATG CGCAGGTTTG CCAAGACCAT CAATAAAGTT 
CTGCTCCAGT ACTCTGAGAT CGTCGCCAAG AGTTTCCAGT CTTACTGCTT CAAGGAGAAA 
CTGCCCTGCA TCCTGATGAA CAACGTGCAG CAACTGAGAG TCCAACTGGA GAAGATGTTT 
GAGGCCATGG GAGGGAAGGA GTTGGATCCC GAGGCCAGCG ACACCCTGAA GGAACTGCAG 
GTCAAACTCA ACACCGTATT GGATGAACTG AGCACTGTGT TTGGGAACAG CTTCCAGTTC 
CGCATAGACG AGTGTATAAA GCAGATGGCA GAGATCCTGG GACACGTGAA GGGCACAGGG 
AATGTGGCAG CCAATGCCAG GAACACGGTG GCCCAGGATG CAGATAATGT ACTGAGGCCC 
CTGATGGATT TCCTGGATGG GAATCTGACC CTGTTTGCCA CGGTGTGTGA GAAGACGGTG 
CTGAAGCGGG TGCTGAAGGA GTTATGGCGA GTAGTAATGA ACACCATGGA GAAGATGATT 
GTGCTCCCGC CCCTTACGGA CCAGTCGGGG ACTCAGATGA TTTTCAGTGC AGCCAAGGAG 
TTGGGCCATT TATCCAAACT GAAGGATCAC ATGGTGAGAG AAGAGACGCG CAGCCTGACC 
CCCAAGCAGT GCGCTGTGAT GGACCTCGCT CTCGATACCA TCAAGCAATA CTTCCACGCC 
GGTGGCAATG GCCTGAAGAA GACCTTTCTG GAGAAAAGTT CAGAACTCCA GTCTCTGCGC 
TACGCCTTGT CCTTGTACAC ACAGACCACA GACACACTCA TCAAAACCTT TGTGCAAACA 
CAGACGTCTC AGGGTTCTGG TGTGGAGGAT CCAGTGGGAG AAGTTTCCAT ACAGGTTGAC 
TTGTTCACAC ACCCAGGGAC TGGGGAGCAG AAGGTGACAG TGAAAGTGGT GGCGGCAAAT 
GATCTAAAAT GGCAGACGAC GGGAATGTTC CGACCCTTCG TGGAGGTGAC AATGATCGGC 
CCCCACCAGA GCGACAAGAA GAGAAAGTTC ACCACCAAGT CCAAGAGCAA TAACTGGGCG 
CCCAAGTACA ACGAGACGTT CCACTTCATC CTGGGGAACG AGGACGGGCC GGACTGCTAC 
GAGCTGCAGG TGTGCGTGAA GGATTACTGC TTCGCCAGAG AGGACCGCGT GTTGGGCATC 
GCCGTCATGC AGCTGCGCGA CATTGCGGAT AAGGGCAGCT GCGCCTGTTG GTGCCCCCTG 
GGGCGCCGGA TACACATGGA CGACACCGGC CTCACCGTAC TGCGGATCCT CTCCCAGAGA 
ACCAACGACG AGGTGGCGCG GGAATTCGTC AAGCTGAAGT CGGACACTCG CTCCACGGAG 
GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750829.1
CDS	482..6553
Translation	MMLARSKLKRQENGTAFRHRKPSVKIFPFKQSKRQKLALCLREKMKRLLQGPVDKISANHDNQIFPVEIDEDLFVCGIKRTPNIAFQKPVDDMELKEEPKELNKTCVEGPDECLTPSLKLPDEQNSCDHDEGNSSNEEDSEVLSPCQSSGLPSDKQDMSAISITMSWREISEHNSLFCPNIPLDPDRESTGSSDRSGDVVEYIIKELQGISRIQAEIAELRQHLSLIKGSVEEVSTCVDSVLNEIEGLQVGVKSPGTASSAELYKDSQNEETVLYFYGVSEHEGENTLDAIHCFLSDHHCFNGVQSKEYIKDAYRLGNGASGLLDPRPTVVRLAHREQRDFILQNSVRLQSAGVRIVPFEEHNIQGLQSRNRANSMSVLQQGLRENRWSSVSLDKGVVRPDDSGGDWKTESFRIRYKNSSTETQCAGEEFVFAQTSTKCNLTAELEATGSQVCQVKENCELEKSVSHLCIRLDSQESQQNLHITNKPSSMDKANCRCARLLTRSSHLSASSEADEAEIAIQRCCSVVDEHTEGLIKGENELVSCDSRRKFRSTEKINVIIKDPSGSFTSLSFDDTVDKRSDGTVAETLKEVVHIDLDTQDQTGHVFRDFLNQPDENIDIVDIKFYANKLGKALNHFRSALKVVFNKLESPEVFMESKESGFPVHDCDSLASVGTGHFNDSIPTQSSRSNSYSSSSNLKEDAYFVLNRVSSLPHETSIPSLILSPDVDLNSAQPLSQSDETMESSELTEELKEAKPMRLDQVCAETIYLNKCINNFKNVLKEKKKMQRKLLKDIVQETFWVSGEEMTSEESRAEYQTQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLAMSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQGSGVEDPVGEVSIQVDLFTHPGTGEQKVTVKVVAANDLKWQTTGMFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSDTRSTEEGS

Target ORF information:

RefSeq Version	XM_031894969.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894969.1

ORF Insert Sequence:

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GGCTATGTCG 
CTGGTCCAGT CACGGAAAGC CGGTATCAGC TCCGCCATGG CGACCCGCAC GTCTCTGAAG 
GATGAAGACC TGAAAACCCA CGTGTACAAG AAGACGCTAC AGGCGCTGAT CTACCCCATC 
TCCTGCACAA CCCCCCACAA CTTTGAGGTG TGGACGGCCA CGACCCCCAC CTACTGCTAC 
GAGTGTGAGG GGCTGCTGTG GGGTATTGCC CGCCAGGGGA TGCGCTGCTC CGAGTGTGGC 
GTCAAGTGCC ACGAAAAGTG CCAAGACTTG CTCAATGCCG ACTGCTTGCA GCGGGCGGCG 
GAGAAGAGCT CCAAGCATGG AGCAGAGGAT CGCACCCAGA ATATTATCAT GGCCATGAAA 
GATCGGATGA AGATCCGGGA GAGGAACAAG CCGGAGATAT TTGAGGTGAT CAGGGACGTG 
TTTGATGTGG GGAAGGTGGC CCATGCCCAG CAGATGAAAA CTGTCAAGCA GAGCGTGTTG 
GACGGGACCT CAAAGTGGTC TGCGAAGATC ACGATCACCG TGCTGTGCGC TCAGGGCCTA 
CAGGCCAAGG ATAAGACCGG CTCCTCCGAC CCCTACGTCA CCGTGCAGGT GGGAAAGACA 
AAGAAGAGAA CAAAAACCAT TTACGGGAAC CTGAATCCTG TCTGGGAGGA GAAATTCCAC 
TTCGAGTGCC ATAACTCATC GGATCGCATT AAAGTTCGGG TTTGGGATGA AGATGACGAC 
ATCAAGTCCC GCGTGAAGCA GAGACTGAAG CGCGAATCCG ATGACTTCCT GGGACAGACG 
ATCATTGAAG TTCGAACACT GAGCGGCGAG ATGGACGTGT GGTACAACCT AGAGAAGCGA 
ACAGACAAGT CGGCCGTATC CGGCGCCATT CGGCTGCAGA TCAGCGTGGA GATTAAGGGA 
GAGGAGAAGG TGGCGCCCTA CCATATCCAG TACACCTGCC TGCACGAGAA CCTGTTCCAT 
TACCTGACAG ACGTACAAGG AGGCGGGGTT GTGAAGATCC CAGAAGCCAA AGGGGACGAC 
GCCTGGAAGG TTTACTTCGA TGAGACGGCC CAGGAGATAG TGGACGAGTT TGCAATGCGC 
TACGGCATAG AGTCTATTTA CCAAGCCATG ACACACTTTG CTTGCCTTTC ATCCAAGTAC 
ATGTGCCCGG GGGTCCCGGC CGTGATGAGC ACCTTACTCG CCAATATAAA TGCCTACTAT 
GCACACACCA CGGCGTCCAC CAACGTTTCT GCCTCCGACC GCTTCTCTGC CTCCAACTTT 
GGGAAAGAGA GATTCGTCAA ACTCCTGGAC CAGCTGCACA ACTCCCTGCG CATCGATCTG 
TCTATGTACC GGAACAATTT CCCAGCCAGC AGCCCGGATC GCCTACAGGA TTTGAAATCC 
ACCGTGGACC TACTGACAAG TATCACATTC TTCCGCATGA AGGTGCAAGA GCTGCAGAGC 
CCCCCGCGCG CCAGCCAAGT GGTGAGGGAC TGCGTTAAGG CCTGTCTGAA TTCCACCTAC 
GAGTACATCT TCAACAACTG CCACGAGCTG TACAGCCGCG AGTACCAGGC AGACCCGACT 
AAGAAACAGG ATCTGCCCCC CGAGGAGCAG GGACCGTGCA TAAAGAACTT GGATTTCTGG 
CCCAAACTCA TTACGCTGAT CGTTTCCATC ATTGAGGAAG ATAAGAACGC CTATACCCCT 
GTGCTGAAAC AATTTCCCCA GGAGCTGAGT GTCGGGAAGG TCAGTGCCGA GGTCATGTGG 
AACCTTTTTG CCCAGGACAT GAAATACGCC ATGGAAGAGC ACGAGAAGCA CCGGATGTGC 
AAGAGCGCCG ACTACATGAA CCTTCACTTC AAGGTCAAGT GGTTGTACAA CGAGTATGTG 
CGGGACCTGC CTGCCATGAA GGATAAAGTG CCTGAATACC CAGCGTGGTT TGAGCAGTTT 
GTGATGCAGT GGTTGGATGA GAACGAGGAG GTGTCCATAG AATTCCAGAA CGGCGCCTTG 
GAGCGAGACA AGAAAGATGG GTTCCAGCAG ACGTCGGAAC ACGCCCTGTT CTCTTGCTCG 
GTGGTGGACG TCTTCACGCA ACTCAACCAA AGCTTCGAGA TCATTAAGAA GTTGGAGTGT 
CCGGACCCGG TCATCGTGGC GCATTATATG CGCAGGTTTG CCAAGACCAT CAATAAAGTT 
CTGCTCCAGT ACTCTGAGAT CGTCGCCAAG AGTTTCCAGT CTTACTGCTT CAAGGAGAAA 
CTGCCCTGCA TCCTGATGAA CAACGTGCAG CAACTGAGAG TCCAACTGGA GAAGATGTTT 
GAGGCCATGG GAGGGAAGGA GTTGGATCCC GAGGCCAGCG ACACCCTGAA GGAACTGCAG 
GTCAAACTCA ACACCGTATT GGATGAACTG AGCACTGTGT TTGGGAACAG CTTCCAGTTC 
CGCATAGACG AGTGTATAAA GCAGATGGCA GAGATCCTGG GACACGTGAA GGGCACAGGG 
AATGTGGCAG CCAATGCCAG GAACACGGTG GCCCAGGATG CAGATAATGT ACTGAGGCCC 
CTGATGGATT TCCTGGATGG GAATCTGACC CTGTTTGCCA CGGTGTGTGA GAAGACGGTG 
CTGAAGCGGG TGCTGAAGGA GTTATGGCGA GTAGTAATGA ACACCATGGA GAAGATGATT 
GTGCTCCCGC CCCTTACGGA CCAGTCGGGG ACTCAGATGA TTTTCAGTGC AGCCAAGGAG 
TTGGGCCATT TATCCAAACT GAAGGATCAC ATGGTGAGAG AAGAGACGCG CAGCCTGACC 
CCCAAGCAGT GCGCTGTGAT GGACCTCGCT CTCGATACCA TCAAGCAATA CTTCCACGCC 
GGTGGCAATG GCCTGAAGAA GACCTTTCTG GAGAAAAGTT CAGAACTCCA GTCTCTGCGC 
TACGCCTTGT CCTTGTACAC ACAGACCACA GACACACTCA TCAAAACCTT TGTGCAAACA 
CAGACGTCTC AGGGTTCTGG TGTGGAGGAT CCAGTGGGAG AAGTTTCCAT ACAGGTTGAC 
TTGTTCACAC ACCCAGGGAC TGGGGAGCAG AAGGTGACAG TGAAAGTGGT GGCGGCAAAT 
GATCTAAAAT GGCAGACGAC GGGAATGTTC CGACCCTTCG TGGAGGTGAC AATGATCGGC 
CCCCACCAGA GCGACAAGAA GAGAAAGTTC ACCACCAAGT CCAAGAGCAA TAACTGGGCG 
CCCAAGTACA ACGAGACGTT CCACTTCATC CTGGGGAACG AGGACGGGCC GGACTGCTAC 
GAGCTGCAGG TGTGCGTGAA GGATTACTGC TTCGCCAGAG AGGACCGCGT GTTGGGCATC 
GCCGTCATGC AGCTGCGCGA CATTGCGGAT AAGGGCAGCT GCGCCTGTTG GTGCCCCCTG 
GGGCGCCGGA TACACATGGA CGACACCGGC CTCACCGTAC TGCGGATCCT CTCCCAGAGA 
ACCAACGACG AGGTGGCGCG GGAATTCGTC AAGCTGAAGT CGGACACTCG CTCCACGGAG 
GAGGGGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100489443 ( XM_031894970.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750830.1 Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X2, mRNA.

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CloneID	OXa49303
Clone ID Related Accession (Same CDS sequence)	XM_031894970.1
Accession Version	XM_031894970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein unc-13 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GTCGCTGGTC 
CAGTCACGGA AAGCCGGTAT CAGCTCCGCC ATGGCGACCC GCACGTCTCT GAAGGATGAA 
GACCTGAAAA CCCACGTGTA CAAGAAGACG CTACAGGCGC TGATCTACCC CATCTCCTGC 
ACAACCCCCC ACAACTTTGA GGTGTGGACG GCCACGACCC CCACCTACTG CTACGAGTGT 
GAGGGGCTGC TGTGGGGTAT TGCCCGCCAG GGGATGCGCT GCTCCGAGTG TGGCGTCAAG 
TGCCACGAAA AGTGCCAAGA CTTGCTCAAT GCCGACTGCT TGCAGCGGGC GGCGGAGAAG 
AGCTCCAAGC ATGGAGCAGA GGATCGCACC CAGAATATTA TCATGGCCAT GAAAGATCGG 
ATGAAGATCC GGGAGAGGAA CAAGCCGGAG ATATTTGAGG TGATCAGGGA CGTGTTTGAT 
GTGGGGAAGG TGGCCCATGC CCAGCAGATG AAAACTGTCA AGCAGAGCGT GTTGGACGGG 
ACCTCAAAGT GGTCTGCGAA GATCACGATC ACCGTGCTGT GCGCTCAGGG CCTACAGGCC 
AAGGATAAGA CCGGCTCCTC CGACCCCTAC GTCACCGTGC AGGTGGGAAA GACAAAGAAG 
AGAACAAAAA CCATTTACGG GAACCTGAAT CCTGTCTGGG AGGAGAAATT CCACTTCGAG 
TGCCATAACT CATCGGATCG CATTAAAGTT CGGGTTTGGG ATGAAGATGA CGACATCAAG 
TCCCGCGTGA AGCAGAGACT GAAGCGCGAA TCCGATGACT TCCTGGGACA GACGATCATT 
GAAGTTCGAA CACTGAGCGG CGAGATGGAC GTGTGGTACA ACCTAGAGAA GCGAACAGAC 
AAGTCGGCCG TATCCGGCGC CATTCGGCTG CAGATCAGCG TGGAGATTAA GGGAGAGGAG 
AAGGTGGCGC CCTACCATAT CCAGTACACC TGCCTGCACG AGAACCTGTT CCATTACCTG 
ACAGACGTAC AAGGAGGCGG GGTTGTGAAG ATCCCAGAAG CCAAAGGGGA CGACGCCTGG 
AAGGTTTACT TCGATGAGAC GGCCCAGGAG ATAGTGGACG AGTTTGCAAT GCGCTACGGC 
ATAGAGTCTA TTTACCAAGC CATGACACAC TTTGCTTGCC TTTCATCCAA GTACATGTGC 
CCGGGGGTCC CGGCCGTGAT GAGCACCTTA CTCGCCAATA TAAATGCCTA CTATGCACAC 
ACCACGGCGT CCACCAACGT TTCTGCCTCC GACCGCTTCT CTGCCTCCAA CTTTGGGAAA 
GAGAGATTCG TCAAACTCCT GGACCAGCTG CACAACTCCC TGCGCATCGA TCTGTCTATG 
TACCGGAACA ATTTCCCAGC CAGCAGCCCG GATCGCCTAC AGGATTTGAA ATCCACCGTG 
GACCTACTGA CAAGTATCAC ATTCTTCCGC ATGAAGGTGC AAGAGCTGCA GAGCCCCCCG 
CGCGCCAGCC AAGTGGTGAG GGACTGCGTT AAGGCCTGTC TGAATTCCAC CTACGAGTAC 
ATCTTCAACA ACTGCCACGA GCTGTACAGC CGCGAGTACC AGGCAGACCC GACTAAGAAA 
CAGGATCTGC CCCCCGAGGA GCAGGGACCG TGCATAAAGA ACTTGGATTT CTGGCCCAAA 
CTCATTACGC TGATCGTTTC CATCATTGAG GAAGATAAGA ACGCCTATAC CCCTGTGCTG 
AAACAATTTC CCCAGGAGCT GAGTGTCGGG AAGGTCAGTG CCGAGGTCAT GTGGAACCTT 
TTTGCCCAGG ACATGAAATA CGCCATGGAA GAGCACGAGA AGCACCGGAT GTGCAAGAGC 
GCCGACTACA TGAACCTTCA CTTCAAGGTC AAGTGGTTGT ACAACGAGTA TGTGCGGGAC 
CTGCCTGCCA TGAAGGATAA AGTGCCTGAA TACCCAGCGT GGTTTGAGCA GTTTGTGATG 
CAGTGGTTGG ATGAGAACGA GGAGGTGTCC ATAGAATTCC AGAACGGCGC CTTGGAGCGA 
GACAAGAAAG ATGGGTTCCA GCAGACGTCG GAACACGCCC TGTTCTCTTG CTCGGTGGTG 
GACGTCTTCA CGCAACTCAA CCAAAGCTTC GAGATCATTA AGAAGTTGGA GTGTCCGGAC 
CCGGTCATCG TGGCGCATTA TATGCGCAGG TTTGCCAAGA CCATCAATAA AGTTCTGCTC 
CAGTACTCTG AGATCGTCGC CAAGAGTTTC CAGTCTTACT GCTTCAAGGA GAAACTGCCC 
TGCATCCTGA TGAACAACGT GCAGCAACTG AGAGTCCAAC TGGAGAAGAT GTTTGAGGCC 
ATGGGAGGGA AGGAGTTGGA TCCCGAGGCC AGCGACACCC TGAAGGAACT GCAGGTCAAA 
CTCAACACCG TATTGGATGA ACTGAGCACT GTGTTTGGGA ACAGCTTCCA GTTCCGCATA 
GACGAGTGTA TAAAGCAGAT GGCAGAGATC CTGGGACACG TGAAGGGCAC AGGGAATGTG 
GCAGCCAATG CCAGGAACAC GGTGGCCCAG GATGCAGATA ATGTACTGAG GCCCCTGATG 
GATTTCCTGG ATGGGAATCT GACCCTGTTT GCCACGGTGT GTGAGAAGAC GGTGCTGAAG 
CGGGTGCTGA AGGAGTTATG GCGAGTAGTA ATGAACACCA TGGAGAAGAT GATTGTGCTC 
CCGCCCCTTA CGGACCAGTC GGGGACTCAG ATGATTTTCA GTGCAGCCAA GGAGTTGGGC 
CATTTATCCA AACTGAAGGA TCACATGGTG AGAGAAGAGA CGCGCAGCCT GACCCCCAAG 
CAGTGCGCTG TGATGGACCT CGCTCTCGAT ACCATCAAGC AATACTTCCA CGCCGGTGGC 
AATGGCCTGA AGAAGACCTT TCTGGAGAAA AGTTCAGAAC TCCAGTCTCT GCGCTACGCC 
TTGTCCTTGT ACACACAGAC CACAGACACA CTCATCAAAA CCTTTGTGCA AACACAGACG 
TCTCAGGGTT CTGGTGTGGA GGATCCAGTG GGAGAAGTTT CCATACAGGT TGACTTGTTC 
ACACACCCAG GGACTGGGGA GCAGAAGGTG ACAGTGAAAG TGGTGGCGGC AAATGATCTA 
AAATGGCAGA CGACGGGAAT GTTCCGACCC TTCGTGGAGG TGACAATGAT CGGCCCCCAC 
CAGAGCGACA AGAAGAGAAA GTTCACCACC AAGTCCAAGA GCAATAACTG GGCGCCCAAG 
TACAACGAGA CGTTCCACTT CATCCTGGGG AACGAGGACG GGCCGGACTG CTACGAGCTG 
CAGGTGTGCG TGAAGGATTA CTGCTTCGCC AGAGAGGACC GCGTGTTGGG CATCGCCGTC 
ATGCAGCTGC GCGACATTGC GGATAAGGGC AGCTGCGCCT GTTGGTGCCC CCTGGGGCGC 
CGGATACACA TGGACGACAC CGGCCTCACC GTACTGCGGA TCCTCTCCCA GAGAACCAAC 
GACGAGGTGG CGCGGGAATT CGTCAAGCTG AAGTCGGACA CTCGCTCCAC GGAGGAGGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750830.1
CDS	482..6547
Translation	MMLARSKLKRQENGTAFRHRKPSVKIFPFKQSKRQKLALCLREKMKRLLQGPVDKISANHDNQIFPVEIDEDLFVCGIKRTPNIAFQKPVDDMELKEEPKELNKTCVEGPDECLTPSLKLPDEQNSCDHDEGNSSNEEDSEVLSPCQSSGLPSDKQDMSAISITMSWREISEHNSLFCPNIPLDPDRESTGSSDRSGDVVEYIIKELQGISRIQAEIAELRQHLSLIKGSVEEVSTCVDSVLNEIEGLQVGVKSPGTASSAELYKDSQNEETVLYFYGVSEHEGENTLDAIHCFLSDHHCFNGVQSKEYIKDAYRLGNGASGLLDPRPTVVRLAHREQRDFILQNSVRLQSAGVRIVPFEEHNIQGLQSRNRANSMSVLQQGLRENRWSSVSLDKGVVRPDDSGGDWKTESFRIRYKNSSTETQCAGEEFVFAQTSTKCNLTAELEATGSQVCQVKENCELEKSVSHLCIRLDSQESQQNLHITNKPSSMDKANCRCARLLTRSSHLSASSEADEAEIAIQRCCSVVDEHTEGLIKGENELVSCDSRRKFRSTEKINVIIKDPSGSFTSLSFDDTVDKRSDGTVAETLKEVVHIDLDTQDQTGHVFRDFLNQPDENIDIVDIKFYANKLGKALNHFRSALKVVFNKLESPEVFMESKESGFPVHDCDSLASVGTGHFNDSIPTQSSRSNSYSSSSNLKEDAYFVLNRVSSLPHETSIPSLILSPDVDLNSAQPLSQSDETMESSELTEELKEAKPMRLDQVCAETIYLNKCINNFKNVLKEKKKMQRKLLKDIVQETFWVSGEEMTSEESRAEYQTQDDGDPSKPPWFKGGPAGGLYGIDSMPDLRKKKPIPLVSDLSLVQSRKAGISSAMATRTSLKDEDLKTHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFDVGKVAHAQQMKTVKQSVLDGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHIQYTCLHENLFHYLTDVQGGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFSASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPDRLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVRDCVKACLNSTYEYIFNNCHELYSREYQADPTKKQDLPPEEQGPCIKNLDFWPKLITLIVSIIEEDKNAYTPVLKQFPQELSVGKVSAEVMWNLFAQDMKYAMEEHEKHRMCKSADYMNLHFKVKWLYNEYVRDLPAMKDKVPEYPAWFEQFVMQWLDENEEVSIEFQNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYMRRFAKTINKVLLQYSEIVAKSFQSYCFKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDPEASDTLKELQVKLNTVLDELSTVFGNSFQFRIDECIKQMAEILGHVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKMIVLPPLTDQSGTQMIFSAAKELGHLSKLKDHMVREETRSLTPKQCAVMDLALDTIKQYFHAGGNGLKKTFLEKSSELQSLRYALSLYTQTTDTLIKTFVQTQTSQGSGVEDPVGEVSIQVDLFTHPGTGEQKVTVKVVAANDLKWQTTGMFRPFVEVTMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDCYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDDTGLTVLRILSQRTNDEVAREFVKLKSDTRSTEEGS

Target ORF information:

RefSeq Version	XM_031894970.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein unc-13 homolog B (LOC100489443), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894970.1

ORF Insert Sequence:

ATGATGTTGG CCAGAAGCAA ACTGAAAAGG CAAGAAAATG GCACTGCCTT CCGGCACAGA 
AAACCCTCGG TAAAAATATT TCCTTTTAAA CAATCCAAAA GGCAAAAACT AGCTCTGTGC 
CTCCGGGAGA AGATGAAACG ATTACTGCAA GGGCCTGTCG ATAAGATTTC AGCAAACCAT 
GATAATCAGA TCTTCCCGGT GGAGATCGAT GAAGACCTGT TTGTGTGCGG GATAAAGAGA 
ACGCCAAACA TTGCATTTCA GAAACCTGTT GACGATATGG AGCTGAAGGA AGAGCCCAAA 
GAGTTAAATA AGACTTGTGT TGAAGGACCG GATGAATGTT TAACACCAAG TCTGAAATTG 
CCAGACGAGC AAAACAGTTG TGACCACGAC GAGGGCAACT CCTCAAATGA GGAAGACAGT 
GAAGTCCTAT CCCCGTGCCA AAGCAGTGGC CTCCCCTCCG ATAAGCAGGA TATGTCTGCC 
ATTAGTATCA CAATGAGCTG GAGAGAGATT TCTGAACACA ATTCACTGTT TTGTCCCAAT 
ATTCCTTTGG ACCCTGACAG GGAAAGCACC GGCAGTAGTG ACCGTTCCGG GGACGTAGTC 
GAATACATCA TCAAGGAGCT TCAAGGAATA AGCCGAATTC AGGCCGAGAT TGCGGAACTA 
AGGCAACATT TGAGCTTGAT TAAAGGGTCA GTGGAAGAGG TTTCCACTTG TGTTGATAGT 
GTCCTTAATG AGATTGAGGG CTTGCAAGTT GGCGTAAAAA GCCCTGGCAC GGCCTCAAGT 
GCTGAGCTCT ATAAAGACTC TCAAAATGAA GAGACCGTTT TGTACTTCTA TGGTGTCTCA 
GAACACGAGG GGGAGAACAC GTTGGATGCC ATCCATTGTT TCTTATCTGA TCACCACTGC 
TTTAATGGCG TTCAAAGCAA AGAATACATA AAGGATGCCT ATAGGCTGGG GAATGGGGCA 
AGCGGTTTGT TAGACCCCAG ACCCACAGTA GTTAGACTGG CTCACCGTGA GCAGAGGGAC 
TTTATTTTAC AAAATTCCGT GCGTTTACAG AGTGCCGGAG TGAGGATCGT TCCATTTGAA 
GAGCACAACA TTCAAGGTCT ACAAAGTCGG AATAGAGCTA ATTCAATGTC TGTTCTCCAG 
CAAGGTTTGA GGGAGAATCG GTGGAGCTCT GTGAGCCTTG ATAAGGGTGT GGTACGTCCC 
GATGACTCTG GGGGTGATTG GAAAACTGAG TCGTTTCGTA TTCGATATAA GAATTCCAGC 
ACCGAGACTC AGTGCGCGGG GGAAGAGTTT GTGTTTGCAC AAACGTCAAC AAAATGCAAC 
TTAACAGCAG AGCTGGAGGC AACTGGAAGT CAAGTGTGTC AGGTCAAAGA GAACTGTGAA 
CTGGAGAAAA GTGTCAGCCA CCTGTGTATT CGCCTGGACT CTCAGGAGTC TCAACAGAAC 
CTTCACATTA CCAACAAGCC TTCATCCATG GACAAGGCCA ACTGTAGGTG TGCCCGTCTT 
TTAACCCGTT CCAGCCACCT CAGTGCCTCC TCTGAGGCAG ATGAAGCAGA AATAGCAATA 
CAGAGATGTT GCTCGGTTGT AGATGAGCAC ACCGAGGGGT TAATTAAGGG AGAAAACGAA 
CTGGTTTCCT GCGACTCGCG GAGAAAATTT AGAAGCACCG AGAAGATAAA TGTGATTATC 
AAAGATCCTT CCGGGAGTTT TACTTCCCTA AGCTTTGACG ACACGGTAGA CAAACGTTCC 
GATGGAACTG TGGCGGAAAC CTTGAAGGAA GTGGTCCACA TAGACCTGGA CACCCAAGAT 
CAAACCGGAC ATGTATTCAG GGATTTCCTC AATCAGCCTG ATGAGAACAT TGATATAGTA 
GATATAAAGT TCTATGCTAA TAAACTGGGC AAAGCTCTTA ATCATTTTAG ATCTGCTCTA 
AAAGTGGTTT TCAATAAGCT CGAAAGTCCT GAGGTCTTTA TGGAGAGCAA AGAGAGTGGA 
TTTCCAGTGC ATGACTGTGA CAGTTTAGCC AGTGTGGGCA CCGGGCACTT CAATGACAGT 
ATCCCCACTC AGTCAAGCAG AAGCAACAGC TACAGTTCCA GCTCCAATCT CAAGGAAGAT 
GCCTATTTTG TCCTCAACAG GGTCTCCTCT CTACCTCATG AGACTTCTAT CCCTTCCTTG 
ATCCTTTCCC CCGATGTAGA CTTGAATTCT GCACAGCCCC TGTCACAGTC GGACGAGACC 
ATGGAGTCCA GCGAACTGAC AGAAGAGCTG AAGGAAGCCA AACCCATGAG ACTCGATCAG 
GTGTGCGCGG AGACCATATA CCTGAACAAG TGCATCAACA ACTTCAAGAA CGTGCTGAAG 
GAGAAGAAGA AGATGCAACG GAAGCTCCTG AAGGACATTG TGCAGGAGAC GTTCTGGGTC 
TCTGGAGAAG AAATGACTTC AGAGGAGAGT AGAGCAGAAT ATCAGACACA AGATGATGGG 
GATCCGTCTA AGCCACCGTG GTTTAAAGGA GGACCGGCGG GAGGGCTCTA TGGCATCGAC 
AGTATGCCCG ATCTGCGCAA GAAGAAGCCC ATCCCTCTGG TCAGCGACTT GTCGCTGGTC 
CAGTCACGGA AAGCCGGTAT CAGCTCCGCC ATGGCGACCC GCACGTCTCT GAAGGATGAA 
GACCTGAAAA CCCACGTGTA CAAGAAGACG CTACAGGCGC TGATCTACCC CATCTCCTGC 
ACAACCCCCC ACAACTTTGA GGTGTGGACG GCCACGACCC CCACCTACTG CTACGAGTGT 
GAGGGGCTGC TGTGGGGTAT TGCCCGCCAG GGGATGCGCT GCTCCGAGTG TGGCGTCAAG 
TGCCACGAAA AGTGCCAAGA CTTGCTCAAT GCCGACTGCT TGCAGCGGGC GGCGGAGAAG 
AGCTCCAAGC ATGGAGCAGA GGATCGCACC CAGAATATTA TCATGGCCAT GAAAGATCGG 
ATGAAGATCC GGGAGAGGAA CAAGCCGGAG ATATTTGAGG TGATCAGGGA CGTGTTTGAT 
GTGGGGAAGG TGGCCCATGC CCAGCAGATG AAAACTGTCA AGCAGAGCGT GTTGGACGGG 
ACCTCAAAGT GGTCTGCGAA GATCACGATC ACCGTGCTGT GCGCTCAGGG CCTACAGGCC 
AAGGATAAGA CCGGCTCCTC CGACCCCTAC GTCACCGTGC AGGTGGGAAA GACAAAGAAG 
AGAACAAAAA CCATTTACGG GAACCTGAAT CCTGTCTGGG AGGAGAAATT CCACTTCGAG 
TGCCATAACT CATCGGATCG CATTAAAGTT CGGGTTTGGG ATGAAGATGA CGACATCAAG 
TCCCGCGTGA AGCAGAGACT GAAGCGCGAA TCCGATGACT TCCTGGGACA GACGATCATT 
GAAGTTCGAA CACTGAGCGG CGAGATGGAC GTGTGGTACA ACCTAGAGAA GCGAACAGAC 
AAGTCGGCCG TATCCGGCGC CATTCGGCTG CAGATCAGCG TGGAGATTAA GGGAGAGGAG 
AAGGTGGCGC CCTACCATAT CCAGTACACC TGCCTGCACG AGAACCTGTT CCATTACCTG 
ACAGACGTAC AAGGAGGCGG GGTTGTGAAG ATCCCAGAAG CCAAAGGGGA CGACGCCTGG 
AAGGTTTACT TCGATGAGAC GGCCCAGGAG ATAGTGGACG AGTTTGCAAT GCGCTACGGC 
ATAGAGTCTA TTTACCAAGC CATGACACAC TTTGCTTGCC TTTCATCCAA GTACATGTGC 
CCGGGGGTCC CGGCCGTGAT GAGCACCTTA CTCGCCAATA TAAATGCCTA CTATGCACAC 
ACCACGGCGT CCACCAACGT TTCTGCCTCC GACCGCTTCT CTGCCTCCAA CTTTGGGAAA 
GAGAGATTCG TCAAACTCCT GGACCAGCTG CACAACTCCC TGCGCATCGA TCTGTCTATG 
TACCGGAACA ATTTCCCAGC CAGCAGCCCG GATCGCCTAC AGGATTTGAA ATCCACCGTG 
GACCTACTGA CAAGTATCAC ATTCTTCCGC ATGAAGGTGC AAGAGCTGCA GAGCCCCCCG 
CGCGCCAGCC AAGTGGTGAG GGACTGCGTT AAGGCCTGTC TGAATTCCAC CTACGAGTAC 
ATCTTCAACA ACTGCCACGA GCTGTACAGC CGCGAGTACC AGGCAGACCC GACTAAGAAA 
CAGGATCTGC CCCCCGAGGA GCAGGGACCG TGCATAAAGA ACTTGGATTT CTGGCCCAAA 
CTCATTACGC TGATCGTTTC CATCATTGAG GAAGATAAGA ACGCCTATAC CCCTGTGCTG 
AAACAATTTC CCCAGGAGCT GAGTGTCGGG AAGGTCAGTG CCGAGGTCAT GTGGAACCTT 
TTTGCCCAGG ACATGAAATA CGCCATGGAA GAGCACGAGA AGCACCGGAT GTGCAAGAGC 
GCCGACTACA TGAACCTTCA CTTCAAGGTC AAGTGGTTGT ACAACGAGTA TGTGCGGGAC 
CTGCCTGCCA TGAAGGATAA AGTGCCTGAA TACCCAGCGT GGTTTGAGCA GTTTGTGATG 
CAGTGGTTGG ATGAGAACGA GGAGGTGTCC ATAGAATTCC AGAACGGCGC CTTGGAGCGA 
GACAAGAAAG ATGGGTTCCA GCAGACGTCG GAACACGCCC TGTTCTCTTG CTCGGTGGTG 
GACGTCTTCA CGCAACTCAA CCAAAGCTTC GAGATCATTA AGAAGTTGGA GTGTCCGGAC 
CCGGTCATCG TGGCGCATTA TATGCGCAGG TTTGCCAAGA CCATCAATAA AGTTCTGCTC 
CAGTACTCTG AGATCGTCGC CAAGAGTTTC CAGTCTTACT GCTTCAAGGA GAAACTGCCC 
TGCATCCTGA TGAACAACGT GCAGCAACTG AGAGTCCAAC TGGAGAAGAT GTTTGAGGCC 
ATGGGAGGGA AGGAGTTGGA TCCCGAGGCC AGCGACACCC TGAAGGAACT GCAGGTCAAA 
CTCAACACCG TATTGGATGA ACTGAGCACT GTGTTTGGGA ACAGCTTCCA GTTCCGCATA 
GACGAGTGTA TAAAGCAGAT GGCAGAGATC CTGGGACACG TGAAGGGCAC AGGGAATGTG 
GCAGCCAATG CCAGGAACAC GGTGGCCCAG GATGCAGATA ATGTACTGAG GCCCCTGATG 
GATTTCCTGG ATGGGAATCT GACCCTGTTT GCCACGGTGT GTGAGAAGAC GGTGCTGAAG 
CGGGTGCTGA AGGAGTTATG GCGAGTAGTA ATGAACACCA TGGAGAAGAT GATTGTGCTC 
CCGCCCCTTA CGGACCAGTC GGGGACTCAG ATGATTTTCA GTGCAGCCAA GGAGTTGGGC 
CATTTATCCA AACTGAAGGA TCACATGGTG AGAGAAGAGA CGCGCAGCCT GACCCCCAAG 
CAGTGCGCTG TGATGGACCT CGCTCTCGAT ACCATCAAGC AATACTTCCA CGCCGGTGGC 
AATGGCCTGA AGAAGACCTT TCTGGAGAAA AGTTCAGAAC TCCAGTCTCT GCGCTACGCC 
TTGTCCTTGT ACACACAGAC CACAGACACA CTCATCAAAA CCTTTGTGCA AACACAGACG 
TCTCAGGGTT CTGGTGTGGA GGATCCAGTG GGAGAAGTTT CCATACAGGT TGACTTGTTC 
ACACACCCAG GGACTGGGGA GCAGAAGGTG ACAGTGAAAG TGGTGGCGGC AAATGATCTA 
AAATGGCAGA CGACGGGAAT GTTCCGACCC TTCGTGGAGG TGACAATGAT CGGCCCCCAC 
CAGAGCGACA AGAAGAGAAA GTTCACCACC AAGTCCAAGA GCAATAACTG GGCGCCCAAG 
TACAACGAGA CGTTCCACTT CATCCTGGGG AACGAGGACG GGCCGGACTG CTACGAGCTG 
CAGGTGTGCG TGAAGGATTA CTGCTTCGCC AGAGAGGACC GCGTGTTGGG CATCGCCGTC 
ATGCAGCTGC GCGACATTGC GGATAAGGGC AGCTGCGCCT GTTGGTGCCC CCTGGGGCGC 
CGGATACACA TGGACGACAC CGGCCTCACC GTACTGCGGA TCCTCTCCCA GAGAACCAAC 
GACGAGGTGG CGCGGGAATT CGTCAAGCTG AAGTCGGACA CTCGCTCCAC GGAGGAGGGG 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100489443 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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サポート

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